Bubble Trap Research Articles

Pattern Uniformity in Large-Area Ultraviolet Nano-Imprinting by a Cylindrically Inflated Flexible Mold Under Low Pressure

This paper shows a novel nano-imprint method with a polydimethylsiloxane (PDMS) replica mold that was bonded on a cylindrically inflated polycarbonate (PC) film via a low air pressure. The PDMS mold, which was deformed in terms of its cylindrical shape, made a line contact with a substrate from the center region and the contact region, then expanded gradually to the outside of the substrate when the contact force increased. This contact procedure squeezed the resin that was dropped on the substrate from the center to the outside, which prevented the trapping of air bubbles while the cavities were filled with the patterns on the PDMS mold. The main characteristic of the proposed process was that the nano-imprint can be realized under a low pressure, compared to conventional processes. We will show the system that was implemented under the proposed process concept and the patterns that were transferred in an ultraviolet curable resin under pressure conditions of less than 5 kPa.

In defense of a fluctuating-interface, particle-accretion origin of folia

Two recent papers have proposed radically different modes of origin for cave folia. Audra et al. (2009) propose subaqueous origin of carbonate folia via hypogenic CO2 bubble trapping, with concurrent condensation-corrosion and evaporative precipitation within individual folia gas pockets. Queen (2009) proposes that at least some folia are analogous to suboceanic tufa-tower "flanges" and may result from subaqueous freshwater mixing into a briny environment. The purpose of this paper is to show that neither of these mechanisms can be the fundamental process responsible for folia morphology in cave deposits, and that accretion from adherent particles at fluctuating interfaces is the only mechanism that has been shown to apply to folia of all compositions and in all cave environments where they are known to occur.

Estimating rates of decompression from textures of erupted ash particles produced by 1999-2006 eruptions of Tungurahua volcano, Ecuador

Persistent low- to moderate-level eruptive activity of andesitic volcanoes is difficult to monitor because small changes in magma supply rates may cause abrupt transitions in eruptive style. As direct measurement of magma supply is not possible, robust techniques for indirect measurements must be developed. Here we demonstrate that crystal textures of ash particles from 1999 to 2006 Vulcanian and Strombolian eruptions of Tungurahua volcano, Ecuador, provide quantitative information about the dynamics of magma ascent and eruption that is difficult to obtain from other monitoring approaches. We show that the crystallinity of erupted ash particles is controlled by the magma supply rate (MSR); ash erupted during periods of high magma supply is substantially less crystalline than during periods of low magma supply. This correlation is most easily explained by efficient degassing at very low pressures (<<50 MPa) and degassing-driven crystallization controlled by the time available prior to eruption. Our data also suggest that the observed transition from intermittent Vulcanian explosions at low MSR to more continuous periods of Strombolian eruptions and lava fountains at high MSR can be explained by the rise of bubbles through (Strombolian) or trapping of bubbles beneath (Vulcanian) vent-capping, variably viscous (and crystalline) magma.

Gas breakthrough at a porous screen

We consider the dynamic breakthrough of a large bubble which is trapped below a porous screen and exposed to a liquid flow. Due to gravity, the bubble is rather flat and blocks part of the screen. Upward liquid flow across the screen leads to a pressure difference between its front and back side. As this pressure difference exceeds the bubble point threshold, the trapped bubble starts to break through the screen causing a number of small bubbles to emerge in chains from the upper side. These emerging bubbles form and detach in their typical manner. They affect the breakthrough and even bring it to a halt as the pressure difference falls below a detachment pressure which is intrinsic to the screen pores. During breakthrough, the trapped bubble shrinks and correspondingly blocks the screen to a lesser extent. The liquid flow around the trapped bubble deforms it and tends to make it flatter. The interplay of all these effects is a complex issue that is addressed in the present article. We develop a model comprising most of the effects and present it along with original experimental data.

Systematic prevention of bubble formation and accumulation for long-term culture of pancreatic islet cells in microfluidic device.

Reliable long-term cell culture in microfluidic system is limited by air bubble formation and accumulation. In this study, we developed a bubble removal system capable of both trapping and discharging air bubbles in a consistent and reliable manner. Combined with PDMS (Polydimethylsiloxane) hydrophilic surface treatment and vacuum filling, a microfluidic perifusion system equipped with the bubble trap was successfully applied for long-term culture of mouse pancreatic islets with no bubble formation and no flow interruption. In addition to demonstrating normal cell viability and islet morphology, post-cultured islets exhibited normal insulin secretion kinetics, intracellular calcium signaling, and changes in mitochondrial potentials in response to glucose challenge. This design could be easily adapted by other microfluidic systems due to its simple design, ease of fabrication, and portability.

Bubbles no more: in-plane trapping and removal of bubbles in microfluidic devices

Gas bubbles present a frequent challenge to the on-chip investigation and culture of biological cells and small organs. The presence of a single bubble can adversely impair biological function and often viability as it increases the wall shear stress in a liquid-perfused microchannel by at least one order of magnitude. We present a microfluidic strategy for in-plane trapping and removal of gas bubbles with volumes of 0.1-500 nL. The presented bubble trap is compatible with single-layer soft lithography and requires a footprint of less than ten square millimetres. Nitrogen bubbles were consistently removed at a rate of 0.14 μL min(-1). Experiments were complemented with analytical and numerical models to comprehensively characterize bubble removal for liquids with different wetting behaviour. Consistent long-term operation of the bubble trap was demonstrated by removing approximately 4000 bubbles during one day. In a case study, we successfully applied the bubble trap to the on-chip investigation of intact small blood vessels. Scalability of the design was demonstrated by realizing eight parallel traps at a total removal rate of 0.9 μL min(-1) (measured for nitrogen).

Roles of gas in capillary filling of nanoslits

Control and understanding of flows inside fabricated nanochannels is rich in potential applications, but nanoscale physics of fluids remains to be clarified even for the simple case of spontaneous capillary filling. This paper reports an experimental and modelling investigation of the role of gas on the capillary filling kinetics slowdown in nanoslits (depth going from 20 nm to 400 nm) compared to Washburn's prediction. First, the role of gas through the usually observed trapped bubbles during a nanoslits capillary filling is analysed thanks to experiments realized with water, ethanol and silicone oil in silicon-glass nanochannels. Bubbles are trapped only when slit depth is below a liquid-dependent threshold. This is interpreted as possible contact line pinning strength varying with wettability. Stagnant trapped bubbles lifetime is investigated for the three liquids used. Experimental results show that bubbles are first compressed because of the increasing local liquid pressure. Once the gas bubble pressure is sufficiently high, gas dissolution induces the final bubble collapse. Influence of the bubbles’ presence on the capillary filling kinetics is analysed by estimating viscous resistance induced by the bubbles using an effective medium approach (Brinkman approximation). Surprisingly, the bubbles’ presence is found to have a very minor effect on nanoslits capillary filling kinetics. Second, the transient gas pressure profile between the advancing meniscus and the channel exit is computed numerically taking into account gas compressibility. A non-negligible over-pressure ahead of the meniscus is found for nano-scale slit capillary filling. Considering the possible presence of precursor films, reducing cross-section for gas flow, leads to a capillary filling kinetics slowdown comparable to the ones measured experimentally.

Dissolution-guided wetting for microarray and microfluidic devices

The trapping of air bubbles presents a substantial impediment for the user in the increasingly widespread use of lab-on-a-chip products having microcavities in the forms of microwells, traps, dead ends and corners. Here we demonstrate a simple, effective, and passive method to eliminate air bubbles by coating hydrophilized microarray and microfluidic devices with a monosaccharide such as D-glucose or D-sorbitol, where the microcavities are filled with a conformal, elliptical, cone-shaped monosaccharide solid. These devices were stored in air for up to 6 months with a complete rewetting of the microcavities by dissolution of the monosaccharide with an aqueous solution.

Bubble-promoted assembly of hierarchical, porous Ag2S nanoparticle membranes

Controlled assembly of nanoparticles can lead to a variety of interesting structures and also is an important step in exploring potential applications. Here, we report a solution process for the self-assembly of macroporous, single-layer to hierarchical Ag2S nanoparticle membranes at the air–liquid interface, in which the trapping and releasing of air bubbles leads to the formation of a three-dimensional porous structure without the need to use a template. The resulting membranes contain close-arranged arrays of polygonal holes (mostly pentagons and hexagons) of a few micrometers in size, which are unanimously open upwards and are surrounded by thin nanoparticle walls. Energy and environmental-related applications are demonstrated based on these multifunctional Ag2S membranes, including photo-sensing, photocatalysis and photoelectrochemical solar cells. The porous nanoparticle membranes are distinct from other extensively studied structures such as densely packed crystal-like films and superlattices.

Motion of Bubbles in Branched-Flow Channel Filled with Viscoelastic Fluids under Pressure-Oscillating Field

In-line defoaming of highly viscous fluids is one of the remaining challenges that needs to be addressed for realizing their industrial use. To overcome the problem of air bubbles in such fluids, the pressure-oscillating defoaming for shear-thinning fluids has been developed. This method is performed by intentionally applying pressure oscillation to a fluid containing a bubble to produce a continuous and strong local flow around the alternately contracting/expanding bubble, where the shear viscosity has been lowered. On the basis of the success that we achieved using this technique for causing bubbles in a quartz cell to rise faster, we applied it to a branched-flow channel filled with a viscoelastic fluid with a zero-shear viscosity of 90 Pa·s for the purpose of developing an in-line defoaming system.In this study, the motions of bubbles in a revised flow channel filled with a higher-viscosity liquid (220 Pa·s) were investigated experimentally. In addition, a small air cavity was introduced on the upper side of the flow channel to act as a bubble trap for improving the defoaming performance. We studied the effect of the trap position on the defoaming performance.

Optofluidic microchannels in aerogel

We report optofluidic waveguides made by filling microchannels in aerogel with water. The aerogel cladding is a nanoporous material with an extremely low refractive index of ~1.05, giving a large index step from the water core. Channels were formed by removing embedded optical fibers, which could be nonuniform or multiple. The porosity of the aerogel allowed air to be displaced from the channel, preventing the trapping of bubbles. The attenuation of red light in the highly multimode water core waveguide was no greater than 1.5 dB/cm.

A comparative study of droplet impact dynamics on a dual-scaled superhydrophobic surface and lotus leaf

The impact dynamics of water droplets on an artificial dual-scaled superhydrophobic surface was studied and compared with that of a lotus leaf with impact velocity V up to 3 m/s. The lower critical impact velocity for the bouncing of droplets was about 0.08 m/s on both surfaces. At relatively low impact velocities, regular rebound of droplets and air bubble trapping and flow jetting on both surfaces were observed as V was increased. For intermediate V, partial pinning and rebound of droplets were found on the artificial dual-scaled surface due to the penetration of the droplets into the micro- and nano-scale roughness. On the lotus leaf, however, the droplets bounced off with intensive vibrations instead of being partially pinned on the surface because of the irregular distribution of microbumps on the leaf. As the impact velocity was sufficiently high, droplet splashing occurred on both surfaces. The contact time and restitution coefficient of the impinging droplets were also measured and discussed.

System-level simulation of liquid filling in microfluidic chips

Liquid filling in microfluidic channels is a complex process that depends on a variety of geometric, operating, and material parameters such as microchannel geometry, flow velocity∕pressure, liquid surface tension, and contact angle of channel surface. Accurate analysis of the filling process can provide key insights into the filling time, air bubble trapping, and dead zone formation, and help evaluate trade-offs among the various design parameters and lead to optimal chip design. However, efficient modeling of liquid filling in complex microfluidic networks continues to be a significant challenge. High-fidelity computational methods, such as the volume of fluid method, are prohibitively expensive from a computational standpoint. Analytical models, on the other hand, are primarily applicable to idealized geometries and, hence, are unable to accurately capture chip level behavior of complex microfluidic systems. This paper presents a parametrized dynamic model for the system-level analysis of liquid filling in three-dimensional (3D) microfluidic networks. In our approach, a complex microfluidic network is deconstructed into a set of commonly used components, such as reservoirs, microchannels, and junctions. The components are then assembled according to their spatial layout and operating rationale to achieve a rapid system-level model. A dynamic model based on the transient momentum equation is developed to track the liquid front in the microchannels. The principle of mass conservation at the junction is used to link the fluidic parameters in the microchannels emanating from the junction. Assembly of these component models yields a set of differential and algebraic equations, which upon integration provides temporal information of the liquid filling process, particularly liquid front propagation (i.e., the arrival time). The models are used to simulate the transient liquid filling process in a variety of microfluidic constructs and in a multiplexer, representing a complex microfluidic network. The accuracy (relative error less than 7%) and orders-of-magnitude speedup (30 000X-4 000 000X) of our system-level models are verified by comparison against 3D high-fidelity numerical studies. Our findings clearly establish the utility of our models and simulation methodology for fast, reliable analysis of liquid filling to guide the design optimization of complex microfluidic networks.

Growing and Analyzing Biofilms in Flow Chambers

This unit describes the setup of flow chamber systems for the study of microbial biofilms, and methods for the analysis of structural biofilm formation. Use of flow chambers allows direct microscopic investigation of biofilm formation. The biofilms in flow chambers develop under hydrodynamic conditions, and the environment can be carefully controlled and easily changed. The protocols in this unit include construction of the flow chamber and the bubble trap, assembly and sterilization of the flow chamber system, inoculation of the flow chambers, running of the system, image capture and analysis, and disassembly and cleaning of the system. In addition, embedding and fluorescent in situ hybridization of flow chamber-grown biofilms are addressed.

Air Removal Efficiency of a Venous Bubble Trap in a Minimal Extracorporeal Circuit During Coronary Artery Bypass

Artificial OrgansVolume 35, Issue 5 p. 555-555 Air Removal Efficiency of a Venous Bubble Trap in a Minimal Extracorporeal Circuit During Coronary Artery Bypass Marco Stehouwer, Marco Stehouwer St. Antonius Ziekenhuis, Department of Extracoporeal Circulation, Koekoekslaan 13435 CM, Nieuwegein, The Netherlands. E-mail: [email protected]Search for more papers by this author Marco Stehouwer, Marco Stehouwer St. Antonius Ziekenhuis, Department of Extracoporeal Circulation, Koekoekslaan 13435 CM, Nieuwegein, The Netherlands. E-mail: [email protected]Search for more papers by this author First published: 20 May 2011 https://doi.org/10.1111/j.1525-1594.2011.01252.xCitations: 1Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume35, Issue5May 2011Pages 555-555 RelatedInformation

Clinical Evaluation of the Air Removal Characteristics of an Oxygenator with Integrated Arterial Filter in a Minimized Extracorporeal Circuit

The use of minimized extracorporeal circuits (MECC) in cardiac surgery is an important measure to increase the biocompatibility of cardiopulmonary bypass during coronary artery bypass grafting (CABG). These circuits eliminate volume storage reservoirs and bubble traps to minimize the circuit. However, the reduction in volume may increase the risk of gaseous microemboli (GME). The MECC system as used by our group consists of a venous bubble trap, centrifugal pump, and an oxygenator. To further reduce the risk of introducing GME, an oxygenator with an integrated arterial filter was developed based on the concept of minimal volume and foreign surface. We studied the air removal characteristics of this oxygenator with and without integrated arterial filter. The quantity and volume of GME were measured with precision at both the inlet and outlet of the devices. Our results showed that integration of an arterial filter into this oxygenator increased GME reducing capacity from 69.2% to 92%. Moreover, we were able to obtain data on the impact of an arterial filter on the exact size-distribution of GME entering the arterial line. The present study demonstrates that an MECC system and oxygenator with integrated arterial filter significantly reduces the volume and size of GME. The use of an integrated arterial filter in an MECC system may protect the patient from the deleterious effects of CPB and may further improve patient safety.

Assessment of Microfluidic System Testability using Fault Simulation and Test Metrics

In this paper we introduce a Microfluidic Fault Simulator, MFS, which uses a novel method of fault modeling and injection, the Fault Block, a generic and low abstraction fault modeling technique. This technique has been utilized over a wide range of fault conditions, in this paper we present a trapped bubble condition. In conjunction with injecting fault conditions, we can apply test methods. Two methods proving sensitive to microfluidic faults are; impedance spectroscopy and Levich electro-chemical sensors, illustrated here by a diffusional "Y" channel mixing system case study. Data from the MFS is analyzed using a Neyman-Pearson probabilistic approach, providing information on each sensor's test capability. Overall fault coverage for a given test is determined. This approach allows the analysis of fault coverage offered by functional-test orientated sensors to be compared to alternative approaches, which potentially offer increased coverage at lower cost.

&lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt; and &lt;em&gt;Saccharomyces cerevisiae&lt;/em&gt; Biofilm in Flow Cells

Many microbial cells have the ability to form sessile microbial communities defined as biofilms that have altered physiological and pathological properties compared to free living microorganisms. Biofilms in nature are often difficult to investigate and reside under poorly defined conditions1. Using a transparent substratum it is possible to device a system where simple biofilms can be examined in a non-destructive way in real-time: here we demonstrate the assembly and operation of a flow cell model system, for in vitro 3D studies of microbial biofilms generating high reproducibility under well-defined conditions2,3. The system consists of a flow cell that serves as growth chamber for the biofilm. The flow cell is supplied with nutrients and oxygen from a medium flask via a peristaltic pump and spent medium is collected in a waste container. This construction of the flow system allows a continuous supply of nutrients and administration of e.g. antibiotics with minimal disturbance of the cells grown in the flow chamber. Moreover, the flow conditions within the flow cell allow studies of biofilm exposed to shear stress. A bubble trapping device confines air bubbles from the tubing which otherwise could disrupt the biofilm structure in the flow cell. The flow cell system is compatible with Confocal Laser Scanning Microscopy (CLSM) and can thereby provide highly detailed 3D information about developing microbial biofilms. Cells in the biofilm can be labeled with fluorescent probes or proteins compatible with CLSM analysis. This enables online visualization and allows investigation of niches in the developing biofilm. Microbial interrelationship, investigation of antimicrobial agents or the expression of specific genes, are of the many experimental setups that can be investigated in the flow cell system.

&lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt; and &lt;em&gt;Saccharomyces cerevisiae&lt;/em&gt; Biofilm in Flow Cells

Many microbial cells have the ability to form sessile microbial communities defined as biofilms that have altered physiological and pathological properties compared to free living microorganisms. Biofilms in nature are often difficult to investigate and reside under poorly defined conditions1. Using a transparent substratum it is possible to device a system where simple biofilms can be examined in a non-destructive way in real-time: here we demonstrate the assembly and operation of a flow cell model system, for in vitro 3D studies of microbial biofilms generating high reproducibility under well-defined conditions2,3.The system consists of a flow cell that serves as growth chamber for the biofilm. The flow cell is supplied with nutrients and oxygen from a medium flask via a peristaltic pump and spent medium is collected in a waste container. This construction of the flow system allows a continuous supply of nutrients and administration of e.g. antibiotics with minimal disturbance of the cells grown in the flow chamber. Moreover, the flow conditions within the flow cell allow studies of biofilm exposed to shear stress. A bubble trapping device confines air bubbles from the tubing which otherwise could disrupt the biofilm structure in the flow cell.The flow cell system is compatible with Confocal Laser Scanning Microscopy (CLSM) and can thereby provide highly detailed 3D information about developing microbial biofilms. Cells in the biofilm can be labeled with fluorescent probes or proteins compatible with CLSM analysis. This enables online visualization and allows investigation of niches in the developing biofilm. Microbial interrelationship, investigation of antimicrobial agents or the expression of specific genes, are of the many experimental setups that can be investigated in the flow cell system.

Experimental study of capillary trapping on the pore scale for various sandstone cores

Abstract Entrapment of the non-wetting phase in porous media has been observed in a variety of fields such as petroleum engineering, geological storage of carbon dioxide, and remediation of ground water. We investigated gas trapping in porous media from a microscopic point of view. High-resolution, three-dimensional images of pore structure and trapped gas bubbles in Berea sandstones were obtained using a micro-focused X-ray CT scanner. We used vertical and horizontal Berea sandstone cores, 8 mm in diameter and 15 mm long. Based on the three-dimensional image analysis, the statistical distribution of the trapped gas volume was estimated. Trapped bubbles have a pore-network scale size and distribute over several pores. In the case of the vertical core, the porosity fluctuates along the flow direction due to the layered structure. The residual gas saturation also fluctuates with porosity along the flow direction. The higher gas saturation in porous layers at the end of gas injection results in a higher trapped gas saturation compared to dense layers. On the other hand, in dense layers the gas saturation at the end of gas injection is almost the same as the residual gas saturation. Therefore, most of the gas injected into the dense layers would be trapped. In the case of horizontal core, the gas saturation at the irreducible water condition is lower than that for the vertical core, because the injected gas selectively passes through the more permeable layers. However, the residual gas saturation is 29.2% for the horizontal core, which is comparable with that for the vertical core (30.9%). Finally, the effect of capillary number on stability of trapped gas bubbles has been estimated. Trapped gas bubbles are stable against the increased flow rate up to a capillary number of 1.0×10 −5 .