The cytoskeleton controls intracellular force equilibrium and force transmission from attachment substrate to focal adhesions (FAs). During epithelial‐mesenchymal transition (EMT), essential process for numerous developmental processes, cell morphology has dramatically change by alter force balance between extracellular matrix (ECM) and cytoskeletons. The force and tension pattern change in cytoskeleton in relation to different morphology is still unknown. Our objective is to simulate the dynamics of intracellular force distribution and total stored energy during cell spreading by using the cytoskeleton tensegrity model with the pre‐tensed actin filaments and the pre‐compressed microtubules. The Madin‐Darby Canine Kidney (MDCK) cell, bovine aortic endothelium cell (BAEC), and myoblastoma cell (C2C12) and NIH 3T3 fibroblast (NIH 3T3) represents each step of EMT. The coordination of FA was used for stimulation. Our results indicated that most of all MDCK and C2C12 is circle shape as compared to BAEC and NIH 3T3 which is polygon. FAs in C2C12 but not BAEC and MDCK fuse together during cell spreading. The total energy in stress fiber and microtubules will increase and decrease with spreading area respectively. Cells have different FA distribution and stress fiber arrangement present different cell morphology due to energy distribution. This study was supported by NSC in Taiwan (NSC 98‐2627‐B‐006‐010).
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