Total Saponin Content Research Articles (Page 1)

Characterization and comparative analysis of saponins in various spinach (Spinacia oleracea L.) cultivars according to growing seasons.

Foliar application of selenium nanoparticles enhance quality and mitigate negative plant-soil feedback in Panax notoginseng by modulating plant-microbiota interactions.

Optimization of Microwave - Assisted Extraction Conditions for Total Saponin Content and Antioxidant Activity of Launaea sarmentosa Leaves

Heat stress (HS), a pervasive environmental stressor, significantly disrupts systemic physiological homeostasis, posing substantial threats to human and animal health. Sheng Mai San (SMS), a classic Traditional Chinese Medicine (TCM) formula, exerts its therapeutic effects by replenishing qi (the vital energy governing physiological functions) and nourishing yin (the material basis responsible for moistening and cooling actions). This formula demonstrates significant efficacy in astringing sweating and preventing collapse. However, its precise molecular mechanisms against HS-induced myocardial injury remain incompletely elucidated. This study initially employed physicochemical analytical methods to determine the contents of total polysaccharides, saponins, and flavonoids in SMS and evaluated its antioxidant activity. Subsequently, both in vitro and in vivo rat models of HS were established to systematically assess the alterations in reactive oxygen species (ROS), antioxidant enzymes (GSH, SOD, CAT), and heat shock proteins (HSP70, HSP90) following SMS intervention, thereby investigating HS-induced myocardial injury and the protective effects of SMS. Furthermore, Western blot, immunofluorescence, and qRT-PCR techniques were utilized to quantitatively analyze key molecules in the Keap1-Nrf2-HO-1 and Stub1-HSF1 signaling pathways. The results demonstrated that total polysaccharides were the most concentrated in SMS, followed by total saponins. This formula exhibited potent free radical scavenging capacity against DPPH, ABTS, and OH−, along with significant reducing activity. HS-induced myocardial injury reached its peak severity at 6-12 h post-stress exposure. SMS intervention effectively suppressed excessive ROS generation, enhanced the activities of antioxidant enzymes (GSH, SOD, and CAT), and downregulated HSP70 and HSP90 mRNA expression levels, thereby significantly mitigating cardiomyocyte damage. Mechanistic investigations revealed that SMS conferred cardioprotection through dual modulation of the Keap1-Nrf2-HO-1 and Stub1-HSF1 signaling pathways. This study not only provides a novel TCM-based therapeutic strategy for preventing and treating HS-related cardiovascular disorders but also establishes a crucial theoretical foundation for further exploration of SMS’s pharmacological mechanisms and clinical applications.

To improve the phosphorus utilization efficiency and medicinal quality of the traditional Chinese medicinal herb Paris polyphylla var. yunnanensis, three inorganic phosphorus-solubilizing bacteria strains (Bacillus cereus Y1-1, Bacillus aryabhattai Z6-1, and Bacillus aryabhattai Z3-4) were inoculated individually and in combination into the rhizosphere soil of Paris polyphylla var. yunnanensis in a greenhouse pot experiment. At the late growth stage, the contents of total and inorganic phosphorus in the rhizosphere soils, as well as the total phosphorus and six steroidal saponins in the plants, were measured. The proportion of inorganic phosphorus in rhizosphere soils of treatment groups increased by 9.83~30.43%. The content of effective phosphorus form Ca2-P in the rhizosphere soils of treatment groups increased by 50.81~328.37%. Inoculation with B. aryabhattai (S2, S3, and S6) significantly increased total steroidal saponin content in plants by 9.14%, 10.64%, and 16.58%, respectively. An antagonistic effect was observed when multiple bacterial strains were inoculated together. Thus, mixed inoculation was less effective than single-strain inoculation in improving rhizosphere soil phosphorus structure. In conclusion, inoculation with inorganic phosphorus-solubilizing bacteria could enhance phosphorus availability in rhizosphere soil and improve medicinal quality of Paris polyphylla var. yunnanensis. These findings provide a basis for efficient phosphorus resource utilization and sustainable cultivation to enhance medicinal plant quality.

ABSTRACTThis study evaluated the diversity of 68 faba bean accessions grown in the Republic of Korea, focusing on variations in antinutrient factors, nutritional composition, fatty acids, lipid quality, antioxidant activity, and key agronomical traits. All parameters showed significant variations, indicating broad genetic diversity. Vicine and convicine were detected in all accessions using UHPLC–MS/MS, with their concentrations varying more than threefold. Total phenol (1.65–4.38 mg GAE/g), saponin (4.12–9.50 mg DE/g), and tannin (1.70–9.47 mg CE/g) contents showed coefficients of variation (CV) above 18.00%. Similarly, crude fiber (3.42%–10.85%), dietary fiber (9.18%–25.47%), total protein (21.68%–34.40%), and total fat (0.90%–1.99%) contents exhibited wide variations (CV > 9.50%). Palmitic acid (15.13%–18.73%) and linoleic acid (38.51%–54.93%) were the dominant saturated and unsaturated fatty acids, respectively. Antioxidant activities, including DPPH• scavenging activity (0.40–2.33 mg AAE/g), ABTS•+ scavenging activity (1.87–7.26 mg TE/g), and ferric reducing antioxidant power (0.67–4.94 mg AAE/g) varied significantly, all with CVs over 25.00%. Green genotypes exhibited significantly higher tannin, fibers, and palmitic and total saturated fatty acids, while yellow genotypes showed higher antioxidant activities, total unsaturated fatty acids, and lipid quality indices. Cluster and principal component analyses differentiated accessions based on their overall biochemical profile. Accession VF050 was identified for its low antinutrient content, VF012 and VF004 for distinct antioxidant potential, VF062, VF027, and VF012 for lipid quality, VF032, VF009, and VF018 for high fiber, VF041 and VF057 for high protein, and VF019 and VF027 for agronomic performance. These accessions could serve as valuable materials for food innovation, crop improvement, and cultivation.

ABSTRACTWorldwide, there is a growing interest in the consumption of wild edible plants that possess high nutritional value and health‐promoting bioactive compounds. Asparagus stands out among these plants due to its cultivated varieties as well as its wild forms. In Türkiye, asparagus consumption has increased more than tenfold over the past 5 years, and it is estimated that nearly half of the consumed asparagus comes from wild species obtained from local markets. Wild asparagus from the Iğdır Plain is in high demand locally and exported to nearby regions. This study was conducted to determine the biochemical composition and evaluate the antioxidant capacities of wild edible asparagus naturally distributed in the Iğdır Plain. A total of 35 wild asparagus spear samples were collected from different regions and analyzed for specific phenolic acids, flavonols, free amino acids, ascorbic acid, total saponin content, and antioxidant activities using DPPH and ABTS methods. According to the results, the wild asparagus samples collected from the Iğdır Plain exhibited higher levels of total phenolic acids, flavonols, amino acids, ascorbic acid, and saponins compared to the commercial cultivated variety. However, when evaluated in terms of antioxidant capacity, the wild samples showed lower activity than the commercial variety in both DPPH and ABTS assays. Statistical analyses revealed a significant level of variation among the wild asparagus genotypes in the region. Notably, samples collected from Iğdır city center, as well as regions A1, A15, A22, A24, and A27, were found to be particularly rich in nutritional and health‐related compounds. This study presents the first scientific data on the biochemical composition and antioxidant capacity of wild edible asparagus naturally growing in the Iğdır region. Furthermore, it is suggested that collecting seeds from promising regions and cultivating these genotypes could help prevent the extinction of these valuable wild species due to overharvesting.

To investigate the effects of the endophytic fungus Setophoma terrestris (isolated from Panax notoginseng roots) on the growth and rhizosphere microbiota of understory-cultivated P. notoginseng, we prepared liquid and solid fermentates of the fungus and applied them separately via irrigation. Rhizosphere soil of P. notoginseng was subjected to non-targeted metabolomics and microbiome sequencing for detection and analysis. Relative to the control, P. notoginseng treated with liquid and solid fermentates exhibited increases in plant height (3.5% and 0.7%), chlorophyll content (23.4% and 20.4%), and total saponin content (14.6% and 17.0%), respectively. Non-targeted metabolomics identified 3855 metabolites across 23 classes, with amino acids and their derivatives (21.54%) and benzene derivatives (14.21%) as the primary components. The significantly altered metabolic pathways shared by the two treatment groups included ABC transporters, purine metabolism, and the biosynthesis of various other secondary metabolites. Exogenous addition of S. terrestris significantly affected the composition of the rhizosphere soil microbial community of P. notoginseng and increased the relative abundance of genera such as Bradyrhizobium. In conclusion, the endophytic fungus S. terrestris enhances P. notoginseng growth and modulates both rhizosphere soil metabolites and microbial abundance. This study can provide certain data support for research on endophytic fungi of P. notoginseng.

The present study investigates the phytochemical and quantitative composition of bioactive ethanolic extracts from five medicinal plants traditionally used in gastrointestinal disorders: Plumbago zeylanica (roots), Moringa oleifera (leaves), Cassia tora (leaves), Amaranthus tricolor (leaves), and Elephantopus scaber (whole plant). The primary aim was to evaluate and compare the total phenolic content (TPC), total flavonoid content (TFC), tannin concentration, and saponin content to identify the most potent extract(s) with antioxidant and therapeutic relevance. All plant materials were subjected to ethanol extraction using a maceration technique followed by qualitative phytochemical screening to detect major secondary metabolites including alkaloids, glycosides, terpenoids, flavonoids, tannins, and saponins. Quantitative estimations were carried out using standard spectrophotometric protocols. Among the evaluated samples, E. scaber exhibited the highest TPC (115.89 ± 2.84 mg GAE/g) and TFC (89.44 ± 2.01 mg QE/g), suggesting it as a rich source of polyphenolic and flavonoid compounds. Tannin content was also highest in E. scaber (47.91 ± 1.65 mg TAE/g), while saponin content peaked at 3.47 ± 0.14%. These phytoconstituents are known for their cytoprotective, anti-inflammatory, and antioxidant properties, which likely contribute to the antiulcer potential observed in subsequent in vivo studies. The findings of this investigation not only validate traditional claims but also provide a scientific rationale for further pharmacological and formulation development. In conclusion, the ethanolic extracts especially that of Elephantopus scaber exhibit significant phytochemical richness and hold promise for future development as herbal antiulcer agents or antioxidant supplements.

Selenium, probiotics, and traditional Chinese medicine (TCM) have shown significant potential in disease prevention and treatment. However, the synergistic interactions between these components and their combined effects on gut health remain underexplored. Here, we developed a novel microecological agent by synthesizing selenium nanoparticles (SeNPs) and fermenting a TCM formulation (Codonopsis pilosula, Astragalus membranaceus, Atractylodes macrocephala, Medicated leaven, Sophora flavescens, Taraxacum mongolicum, and Prunus mume) with probiotics (Bacillus subtilis ACCC 19373, Kluyveromyces lactis GG 799, and Lactobacillus reuteri LR1) in an asynchronous manner. Results showed that fermentation process facilitated the decomposition of TCM cell walls, markedly facilitating the release and bioutilization of active ingredient, while significantly decreasing the contents of soluble total polysaccharide and increasing soluble total saponin contents, total flavonoids, and total alkaloids. During fermentation, sodium selenite was effectively converted into SeNPs by probiotics. The lyophilization protocol for the microecological preparation was optimized by adding 10% skim milk powder, 16% trehalose, and 0.9% sodium acetate at a ratio of 1:2 to the product, yielding a viable probiotic count 19.89 times higher than that of direct lyophilization. Dietary supplementation with the microecological agent significantly alleviated lipopolysaccharide (LPS)-induced intestinal barrier damage, enhanced antioxidant enzyme activities including total superoxide dismutase (T-SOD), thioredoxin reductase (TrxR), and glutathione peroxidase (GPx), upregulated anti-inflammatory cytokines interleukin-10 (IL-10) and transforming growth factor-β (TGF-β), and downregulated pro-inflammatory tumor necrosis factor-α (TNF-α). Moreover, it restored intestinal microbiota homeostasis by enriching beneficial bacteria (e.g., Fusobacteriota, Rikenellaceae_RC9_gut_group) and reducing harmful taxa (e.g., Parasutterella), with correlations of these microbes to inflammatory cytokines and antioxidant markers, suggesting that the protective effects may be mediated through microbiota-driven regulation of inflammation and oxidative stress. These findings demonstrate that this novel microecological agent is a promising strategy for preventing and treating intestinal injury, offering new insights into selenium biotransformation and the therapeutic potential of integrating probiotics with TCM for gut health.

Background/Objective. Sclerotium rolfsii and Sclerotinia sclerotiorum are phytopathogenic fungi of agricultural significance. The use of phytoextracts with antifungal properties offers an alternative approach to reduce agrochemical applications in pathogen management. This study reports the phytochemical characterization of Datura discolor aqueous extracts obtained by infusion and High-Pressure Processing (HPP), as well as their antifungal evaluation. Materials and Methods. Aqueous extracts from the root, stem, seed, and leaf of Datura discolor (2, 4, and 6 % w/v) were obtained by infusion HPP. Phytochemical analysis was conducted through screening tests and quantification of total metabolites using colorimetric assays. The antifungal activity of the extracts obtained by infusion was determined based on the in vitro inhibition percentage of the pathogens. Results. Total phenolics and saponins content in root, stem, and leaf was higher in extracts obtained by HPP, whereas infusion showed greater values in the seed. Flavonoids were observed only in leaf extracts obtained by HPP. Alkaloid content was similar both infusion and HPP extracts. Phenols, saponins, flavonoids, alkaloids, tannins, terpenoids, coumarins, and betacyanins were detected, while anthraquinones and anthocyanins were not. The extracts inhibited Sclerotium rolfsii by 2 to 46 % but showed no effect on Sclerotinia sclerotiorum. Conclusion. The results indicate that phenolic compounds and flavonoids contribute to the antifungal activity against the evaluated phytopathogens; the involvement of other non-analyzed compounds cannot be ruled out. Further studies under greenhouse conditions are required, applying the extracts either as foliar spray or soil treatment.

Quinoa (Chenopodium quinoa) cultivation and consumption have been increasing globally for its nutritional value and agricultural adaptability, with over 120 countries involved in its production. In Canada, quinoa is cultivated as a specialty crop to increase crop diversity and support agroresilience. This study is the first to examine quinoa cultivars grown under northern Quebec conditions and to provide a nutritional and sensory characterization of two Quebec (Canada) varieties (Sweet and Bitter) in comparison to the Bolivian reference cultivar, Royal White. Analyses included proximate composition, amino acids, fatty acids, phenolics, and anti-nutrients. Sensory evaluations involved hedonic and bitterness ranking tests. Bolivian cultivar had higher omega-3 content, while the Quebec cultivars showed favorable protein and lipid profiles, with better lipid health indexes. Protein quality was comparable between the Bolivian and Sweet cultivars. The overall flavor appreciation was similar among twice-brushed Bitter cultivar and Bolivian samples. The Bolivian sample received a better score for texture. Descriptive flavor data support the development of a quinoa flavor lexicon. Notably, total saponins content, commonly used as a bitterness indicator, did not consistently correlate with perceived bitterness, emphasizing the need for a standardized quantification method for cultivar selection and further investigation into other flavor-contributing compounds.

Important secondary metabolites in Melia dubia Cav. were investigated to reveal variations in these secondary phytochemicals across different provenances within the Satpura and northwestern Ghats Mountain ranges. Secondary metabolites were estimated during the winter and summer seasons of 2020 and 2021 to determine their provenance and seasonal variations. Study revealed that the total alkaloid content (TAC), total flavonoids content (TFC), total phenolic content (TPC), total saponin content (TSC), total tannin content (TTC) and total terpenoids content (TTeC) of M. dubia exhibited significant variation (p ≤0.05) among the selected 8 provenances (except TSC) and seasons (winter and summer). TAC, TFC, TSC, TTC, TTeC and TPC ranged from 12.73–13.41, 2.27–2.78, 12.51–16.91, 12.34–12.64, 9.53–16.03 and 7.13–7.76%, respectively. TSC content did not exhibit significant variation among provenances. Leaf fodder TAC, TFC, TSC, TTC and TTeC (14.57, 3.25, 13.01, 15.64 and 8.04%, respectively) was significantly higher in the winter season and TPC was significantly higher (19.44%) in the summer season. The interaction effect due to provenance and season was statistically significant for all attributes. Findings suggested that the winter season i.e. lean period fodder of M. dubia possesses higher secondary metabolites and could be utilized for fodder quality improvement. These secondary metabolites in M. dubia leaf fodder could be a rich source of antioxidant, anti-inflammatory, and antibacterial activity, among others.

Leaves (LV), stems (STs), and inflorescences (IFs) of Pfaffia glomerata are usually discarded despite containing various bioactive compounds, especially β-ecdysone saponin. The objective was to optimize by desirability (DI) the ultrasound-assisted extraction (UAE) of bioactive compounds (total phenolics (TPCs), antioxidant activity (AA), and total saponins) from the aerial parts (LV, ST, and IF) of P. glomerata. Ideal drying conditions were determined and the drying kinetics were evaluated. LV, STs, and IFs were dried and extracted (0.06 g/mL 80% EtOH) in a USS (6 cm × 12 mm, pulse 3/6 s) by Central Composite Design (CCD), varying sonication power (140–560 W) and time (11–139 min), with TPC, AA by DPPH, and total saponin content as responses. The DI indicated that the higher TPC, AA, and saponin levels were obtained at 136.5 min and 137.87 W (STs), and 138.6 min and 562.32 W (LV and IFs). IF extracts contained higher saponin, TPCs, and AA. Higher β-ecdysone levels (3.90 mg g−1) were present in the leaves. Several phenolics were detected in area parts of P. glomerata, the most abundant being p-coumaric acid (LV) and nicotinic acid (STs and IFs). These compounds provide potential health benefits. Phytol was found in all extracts. Extracts by UAE from leaves have antibacterial potential, with demonstrated inhibitory effects against S. aureus, E. coli, L. monocytogenes, S. Typhi, and P. aeruginosa, and presented bactericidal effects against E. coli, L. monocytogenes, and S. Typhi. Aerial parts of P. glomerata can be used to obtain extracts by UAE rich in bioactive compounds, providing complete utilization of the plant and sustainability to cultivation. This work represents the first report on the application of ecofriendly UAE techniques to extract bioactive compounds from the aerial parts of Brazilian ginseng.

Ganoderma neo-japonicum Imazeki, 1939 is a special mushroom species growing on tropical bamboo bushes and has been used in folk medicine to prevent and treat many diseases. Our investigation provided valuable scientific evidence about nutritional composition, active biological compounds, and antioxidant properties, helping to evaluate this mushroom's medicinal value properly and contributing to the conservation and propagation of precious mushroom species in Bu Gia Map National Park. G. neo-japonicum contains a high total polysaccharide of 72.4 %, carbohydrate of 74 g/100 g, and high mineral contents like Na, Ca, and Mg. Additionally, the ethanol extract of G. neo-japonicum exhibits a substantial total polyphenol content, quantified at 284.91 mg GAE/mg DW, along with a total flavonoid content of 73.12 mg CAE/g DW and a total saponin content of 230.23 mg OAE/g DW. Consequently, this extract demonstrates potent antioxidant properties, evidenced by an IC50 value of 11.70 µg/mL in the DPPH assay and an EC50 value of 124.68 µg/mL in the FRAP assay. Interestingly, the presence of adenosine with a concentration of 104 mg/kg plays a significant role in using this mushroom in food and medicine because this is a bioactive compound found in Cordyceps mushrooms and has a variety of bioactivity such as possible cardioprotective and therapeutic agents for chronic heart failure.

Paris polyphylla var. yunnanensis is a widely cultivated rhizomatous perennial woody plant known for producing highly valued steroidal saponins. Steroidal saponins serve as the primary active components and exhibit a variety of pharmacological effects. Their production is closely correlated with the growth environment and developmental stages of P. polyphylla. However, the medicinal value of P. polyphylla cultivated for varying durations remains unclear. In this study, we collected samples of P. polyphylla aged 3 to 8 years for metabolome and transcriptome sequencing analysis. The total saponin content was found to be significantly higher in 8-year-old P. polyphylla compared to younger plants. A total of 1,510 metabolites and 270.65 Gb of clean data were identified from these samples. This study provides insights into the medicinal values of P. polyphylla at different growth stages and elucidates the metabolic pathways for specific steroidal saponins.

Livestock methane emissions are a significant source of greenhouse gases. The aim of this study was to investigate the secondary metabolites of different strains of silage quinoa and their impact on methane emissions from livestock farming. In this study, we evaluated the chemical composition, fermentation quality, secondary metabolite content, and in vitro gas production of eight quinoa lines, 093, 137, 231, 238, 565, 666, 770, and 811, grown in saline and alkaline areas of the Yellow River Delta. The results showed that crude protein, EE, and crude ash content ranged from 8.84% to 10.69%, 1.98% to 2.38%, and 17.00% to 23.14%, respectively. The acidic and neutral detergent fiber content of these eight quinoa varieties ranged from 49.31% to 61.91% and 33.29% to 37.31%, respectively. Line 093 had the highest total saponin content, while Line 231 exhibited the highest flavonoid content. Methane yield was significantly and negatively correlated with tannin, saponin, and flavonoid content, whereas carbon dioxide yield showed a positive correlation with saponin and flavonoid content. Among all lines, 770 and 811 demonstrated the lowest methane production, indicating strong in vitro inhibition of methanogenesis. These findings suggest that feeding quinoa silage to ruminants has the potential to reduce greenhouse gas emissions.

The traditional Chinese herbal medicine Panax ginseng can optimize physical health and is anticipated to be a valuable resource for investigating anti-aging therapies. This study investigated the anti-aging effects of red ginseng aqueous extract (RG) and white ginseng aqueous extract (WG). Network pharmacology forecasted that the key mechanisms of anti-aging in white and red ginsengs were the PI3K-Akt and IIS signaling pathways. Experiments conducted on Caenorhabditis elegans (C. elegans) showed that 5mg/mL WG and RG notably prolonged lifespan and improved stress resistance. The qPCR analysis revealed that changes in upstream genes activated downstream genes in the IIS pathway. Furthermore, forward and reverse validation indicated that WG and RG acted through the IIS pathway in promoting longevity. RG exhibited superior anti-aging effects compared to WG at the same concentration. This might be attributed to the fact that RG contained more reducing sugars, polyphenols, melanoidins, total saponin content, and especially the conversion of ginsenosides. Molecular docking showed that ginsenosides interacted with the key protein DAF-2, with ginsenosides Rg3, Rg5, Rh4, Rf, and Rc binding more strongly than ginsenosides Rb1, Rb2, and Rd. Overall, RG possessed different active ingredients compared to WG and showed superior results in improving aging in C. elegans.

Abstract Soybeans have long been used as an important component of production animal diets, particularly in the form of soybean meal (SBM), and serve as a high-quality source of protein with a nearly complete amino acid profile. Outside of its primary nutritive fractions, SBM contains a multitude of minor bioactive components that have variable effects on animal health. Soy saponins are one such component that were previously lumped into the category of anti-nutritional factors found in SBM but have more recently been investigated for their potential anti-inflammatory and anti-oxidative properties. Challenges with their isolation have limited research to in vitro or rodent models, which only require small quantities of purified saponins. However, these studies are less applicable to understanding how saponins are affecting larger production animals that are the primary consumers of SBM. In this research, a process for creating greater quantities of saponins was evaluated for its practicality and effectiveness. A total of 10 kg SBM was used as the starting material for the extraction protocol, which initially contained 5.30 mg total saponins/g SBM. The saponin profile of the meal was predominantly made up of group B saponins, at 57.6% of total saponins, with limited quantities of group A saponins at only 7.17% of the total. The SBM was subjected to two consecutive rounds of mixing with 75% undenatured ethanol at 40℃ for 24 hr. After these mixes, remaining SBM solids were removed and ethanol was evaporated to obtain 12.4 kg of a soy molasses product consisting of the carbohydrate, isoflavone, and saponin fractions of the original SBM. This molasses product was then acidified under elevated temperatures (40℃) using concentrated hydrochloric acid to a pH of 3.05. Under these conditions, a precipitate formed and was recovered via centrifugation to obtain 494 g of precipitated solids likely consisting of isoflavones and saponins. This precipitate was combined with 80% American Chemical Society grade acetone at a 10:1 ratio. The pH of the solution was elevated to 6.00 using sodium hydroxide and the solution was heated to reflux at 56℃ for 90 min. The solution was chilled to 4℃ at which time a precipitate formed and was recovered using vacuum filtration. Analyses are currently being performed to determine the total saponin and isoflavone content as well as the saponin profile of this final product to ensure that saponins were adequately extracted from the initial SBM and isolated from other bioactive components like isoflavones. Pending these results, this large-scale saponin extraction protocol may be utilized to create a saponin-enriched product that can be used in future live animal trials using larger production animals.

This study aims to evaluate the pharmacological potential and standardize the bioactive compounds of Ngoc Linh Ginseng (Panax vietnamensis) as a candidate phytopharmaceutical. The study was conducted throughout 2024 using a multidisciplinary approach, including ultrasonic extraction, chemical analysis, and pharmacological tests. Samples were collected from the Kon Tum and Quang Nam mountains, Vietnam, then extracted by the Ultrasonic-Assisted Extraction (UAE) method using ethanol-water solvents. The total saponin content obtained was analyzed using High Performance Liquid Chromatography (HPLC), resulting in a level of 28.5% with the identification of the main ginsenosides Rg1 and Re. Pharmacological activity was tested in vitro on LPS-induced RAW 264.7 cells, showing a decrease in pro-inflammatory cytokine production by up to 50% and strong antioxidant activity with an IC50 value of 25 µg/mL. In vivo tests on Wistar rats showed a hepatoprotective effect against liver damage caused by a high-fat diet. These results support the potential of Ngoc Linh Ginseng as a candidate for plant-based therapy. Further studies are recommended to explore the pharmacokinetics, clinical efficacy, and optimization of extraction techniques to expand its therapeutic applications.