Total Fatty Acid Composition Research Articles

Probiotic properties and fatty acid composition of the yeast Kluyveromyces lactis M3. In vivo immunomodulatory activities in gilthead seabream (Sparus aurata)

The aim of this study was to analyze the probiotic potential, fatty acid composition and immunostimulant activities of Kluyveromyces lactis M3 isolated from a hypersaline sediment. For this purpose, K. lactis M3 resistance to different pH, salinities and bile, as well as its antioxidant capability were assayed. Furthermore, total fatty acid composition of the yeast was determined where the dominant fatty acids were palmitic, palmitoleic, oleic and linoleic acids. K. lactis M3 showed no cytotoxic effects on peripheral blood leukocytes. During an in vivo experiment in gilthead seabream (Sparus aurata), dietary K. lactis M3 supplemented at 0.55 or 1.1% of the basal diet enhanced bactericidal activity against Vibrio parahaemolyticus N16, V. harveyi Lg 16/00, and V. anguillarum CECT 43442 compared to fish fed commercial diet (control group). Finally, nitric oxide production, peroxidase activity and skin mucus lectin union levels strongly increased in fish fed K. lactis M3 with respect to the control group. The results suggested that the yeast K. lactis M3 had exhibited high antioxidant capability, and its dietary administration at 0.55 or 1% basal diet had immunostimulant activity for gilthead seabream. For all these reasons, it should be considered an appropriate probiotic candidate for the aquaculture fish industry.

Seed Oil Quality of Brassica napus and Brassica rapa Germplasm from Northwestern Spain.

The seed oil content and the fatty acid composition of a germplasm collection of Brassica napus and Brassica rapa currently grown in Galicia (northwestern Spain) were evaluated in order to identify potentially interesting genotypes and to assess their suitability as oilseed crops for either edible or industrial purposes. The seeds of the B. rapa landraces had higher oil content (mean 47.3%) than those of B. napus (mean 42.8%). The landraces of both species showed a similar fatty acid profile (12% oleic acid, 13% linoleic acid, 8–9% linolenic acid, 8–9% eicosenoic acid, and 50–51% erucic acid). They were very high in erucic acid content, which is nutritionally undesirable in a vegetable oil, and very low in oleic and linoleic acid contents. Therefore, they could be used for industrial purposes but not as edible oil. The erucic acid content ranged from 42% to 54% of the total fatty acid composition with an average value of 50% in the B. napus landraces whereas in B. rapa, it ranged from 43% to 57%, with an average value of 51%. Considering the seed oil and the erucic acid content together, three varieties within the B. napus collection and two varieties within the B. rapa one seem to be the most promising genotypes for industrial purposes.

Primary Metabolism in Avocado Fruit.

Avocado (Persea americana Mill) is rich in a variety of essential nutrients and phytochemicals; thus, consumption has drastically increased in the last 10 years. Avocado unlike other fruit is characterized by oil accumulation during growth and development and presents a unique carbohydrate pattern. There are few previous and current studies related to primary metabolism. The fruit is also quite unique since it contains large amounts of C7 sugars (mannoheptulose and perseitol) acting as transportable and storage sugars and as potential regulators of fruit ripening. These C7 sugars play a central role during fruit growth and development, but still confirmation is needed regarding the biosynthetic routes and the physiological function during growth and development of avocado fruit. Relatively recent transcriptome studies on avocado mesocarp during development and ripening have revealed that most of the oil is synthesized during early stages of development and that oil synthesis is halted when the fruit is harvested (pre-climacteric stage). Most of the oil is accumulated in the form of triacylglycerol (TAG) representing 60–70% in dry basis of the mesocarp tissue. During early stages of fruit development, high expression of transcripts related to fatty acid and TAG biosynthesis has been reported and downregulation of same genes in more advanced stages but without cessation of the process until harvest. The increased expression of fatty acid key genes and regulators such as PaWRI1, PaACP4-2, and PapPK-β-1 has also been reported to be consistent with the total fatty acid increase and fatty acid composition during avocado fruit development. During postharvest, there is minimal change in the fatty acid composition of the fruit. Almost inexistent information regarding the role of organic acid and amino acid metabolism during growth, development, and ripening of avocado is available. Cell wall metabolism understanding in avocado, even though crucial in terms of fruit quality, still presents severe gaps regarding the interactions between cell wall remodeling, fruit development, and postharvest modifications.

Evaluation model for cocoa butter equivalents based on fatty acid compositions and triacylglycerol patterns.

An effective evaluation model was established to digitize the quality of cocoa butter equivalents (CBEs) based on determinations of total and sn-2 fatty acid compositions and triacylglycerol (TAG) profiles and the "deducting score" principle. Similarity scores for selected fats and oils calculated from the model revealed differences between them and parallel cocoa butter compositions. For CBE1 and CBE2, total similarity scores were 90.6 and 90.0, whereas those of mango (76.3), dhupa (84.1), sal fat (84.7), kokum (78.3), palm mid fractions (PMF, 77.9), shea butter (64.0), illipe butter (89.7) and Pentadesma butyracea butter (67.2), respectively. Similarity scores were found to agree with physical properties, including polymorphism, crystal morphology, crystallization or melting behaviors, and solid fat content. The present study provides an accurate means of assessing CBE quality and hopefully will contribute to the development of commercial CBEs.

Alleviation of cadmium-induced genotoxicity and cytotoxicity by calcium chloride in faba bean (Vicia faba L. var. minor) roots.

Alleviation of cadmium-induced root genotoxicity and cytotoxicity by calcium chloride (CaCl2) in faba bean (Vicia faba L. var. minor) seedlings were studied. Faba bean seeds were treated with H2O or 2% CaCl2 for 6h before germination. Seeds were then exposed to 0 and 50µM CdCl2 concentrations for 7days. Genotoxic damaging effects of Cd was examined through the determination of the mitotic index (MI), chromosomal aberrations (CA) and micronucleus (MN) in the meristem cells of faba bean roots. Similarly, effects of Cd stress on metal accumulation, total membrane lipid contents, total fatty acid composition (TFA), lipid peroxidation as indicated by malondialdehyde production, soluble protein and non-protein thiols (NP-SH) contents, hydrogen peroxide production and the activities of superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (GPX) were evaluated after 7days of Cd stress in the seedling roots. Cd stress resulted in the reduction of MI, in addition toMN formation and CA induction in the roots of non-primed seeds (treated with H2O). Moreover, Cd induced lipid peroxidation, H2O2 overproduction and loss of membrane lipid amount and soluble protein content, and changes in the TFA composition in roots of faba bean seedlings. SOD activity declined, but CAT and GPX activities increased. However, seed pre-treatment with CaCl2 attenuated the genotoxic and cytotoxic effects of Cd on Vicia faba roots. The results showed that CaCl2 induced reduction of Cd accumulation, improved cell membrane stability and increased the antioxidant defence systems, thus reducing and alleviating Cd genotoxicity and oxidative damage.

Enzymatic Modification of Menhaden Oil to Incorporate Caprylic and/or Stearic Acid

AbstractMenhaden oil was enzymatically modified with caprylic (8:0) and/or stearic acid (18:0) to produce structured lipids (SL). The goal was to produce SL with high amounts of polyunsaturated fatty acids (PUFA), a low level of saturation, and a melting point of 25–35 °C. Substrate (menhaden oil to acyl donor) molar ratios were 1:1, 1:3, and 1:5 for 8:0, and 1:1, 1:2, and 1:3 for 18:0. Enzyme load was 10% of the total weight of substrates. Time course study determined optimal time for maximum acyl donor incorporation. Linear interpolation estimated molar ratios that yielded SL with 20 or 30 mol% incorporation of 8:0 or 18:0. Enzymatic reactions were also conducted with molar ratios of menhaden oil to acyl donors:8:0:18:0 (1:1:3, 1:2:2, and 1:3:1). Lipases from Candida antarctica, Lipozyme® 435, and Rhizomucor miehei, Lipozyme® RM IM (Novozymes North America, Inc., Franklinton, NC, USA), were compared for all reactions. Total and sn‐2 fatty acid compositions, triacylglycerol (TAG) molecular species, thermal behavior, volatile lipid oxidation products, solid fat contents, and oxidative stability were compared. When 8:0 was the acyl donor, the 1:3.03 and 1:4.58 ratios resulted in incorporation of 20 and 30 mol% 8:0, respectively. With 18:0 as the acyl donor, the 1:1.32 and 1:2.41 ratios led to incorporation of 20 and 30 mol% 18:0, respectively. The 1:3:1 ratio SL had a crystallization onset (C0) of 15.3 °C and a melting completion (Mc) of 33.1 °C. The physicochemical properties of these SL suggest that some may be useful in formulating food products such as margarines and spreads.

The Discrepancy of Fatty Acid Composition of Astaxanthin Esters and Total Fatty Acids in Photoautotrophic and Heterotrophic Chlorella zofingiensis

Abstract Chlorella zofingiensis has great potential for astaxanthin and fatty acid production. This study investigated the accumulation of astaxanthin and total fatty acids (TFA), the fatty acid compositions of astaxanthin esters and TFA in photoautotrophic and heterotrophic C. zofingiensis, respectively. The results displayed that in photoautotrophic cells, 18:3 was the most predominant fatty acid, followed by 16:0, 18:2, and 18:1, while astaxanthin mono‐esters (mono‐AE) contained only 16:0 and 18:0, and astaxanthin di‐esters (di‐AE) contained 16:0 mostly, followed by 18:1, 18:0, 18:2, and 18:3. C. zofingiensis accumulated the highest level of astaxanthin and TFA under heterotrophic conditions fed with 30 g L−1 glucose. The percentage of 18:1 in TFA, mono‐AE, and di‐AE increased from 10.05%, 0%, and 17.76% to 35.80%, 14.84%, and 50.12%, respectively; in contrast, the percentages of 18:3 in TFA and di‐AE were observed to decrease from 40.42% and 7.59% to 9.56% and 1.30%, respectively, in comparison with those in photoautotrophic ones. The RNA sequencing (RNA‐seq) results showed that the expressions of the genes in astaxanthin and fatty acid biosynthetic pathways were increased under this heterotrophic condition. These results indicated differential accumulation of AE and TFA, especially fatty acid compositions of TFA and AE in photoautotrophic and heterotrophic cells, suggesting a positive correlation between astaxanthin and fatty acid biosynthesis.

Effect of three diets on the gametogenic development and fatty acid profile of Paracentrotus lividus (Lamarck, 1816) gonads

In this study, the effects of three diets were investigated to enhance Paracentrotus lividus production for commercial purposes. P. lividus were fed ad libitum for 80 days with: diet A—fresh Codium tomentosum Stackhouse, 1797; diet B—formulated using a jellified mix of macroalgae and vegetables, including C. tomentosum (20%), Coralina sp. Linnaeus, 1758 (17%), cabbage Brassica oleracea var. capitata Linnaeus, 1753 (30%), carrot Daucus carota Linnaeus, 1753 (30%) and agar (3%) as a gelling agent. Diet C consisted of maize Zea mays Linnaeus, 1753 (56%) and New Zealand spinach Tetragonia tetragonoides (Pallas, 1781) Kuntze, 1891 (44%). Their effects on the gonadal and somatic growths, gonadosomatic index (GI) and gametogenesis were evaluated, as well as on the total lipid content and fatty acid composition of sea urchin's gonads. Diet A provided high values of eicosapentaenoic acid (EPA). Gonads of sea urchins fed with diet A were found mostly in growth and maturation stages of gametogenesis and showed the lowest lipid content. Sea urchins fed with diet B presented their gonads in the reabsorption stage and had the highest values of omega-3 polyunsaturated fatty acids (PUFAs). Sea urchins fed with diet C were in the early stages of gametogenesis and had the highest values of lipid content, plus omega-6 PUFAs. Once as an ingredient in a balanced mix with vegetables, C. tomentosum can be a key factor to the development of new promising high-quality and low-cost feed for P. lividus roe enhancement.

Geographical variations in the fatty acids of Zanthoxylum seed oils: A chemometric classification based on the random forest algorithm

Some Zanthoxylum species have the potential to develop oil-oriented wood plants. But confusion of seed oils and low environmental adaptability of fatty acid lead to obstacles to their further development. In this work, a total of 99 seed samples of two widely distributed Zanthoxylum species (Zanthoxylum bungeanum and Zanthoxylum armatum) seeds were collected from 97 plantations located at 32 locations in China spread over a wide geographic area (a range of 17.2°longitude and 12.8°latitude) and fatty acid composition of seed oils extracted by supercritical CO2 extractor apparatus among individuals was determined. The random forest algorithm was employed to conduct a classification model to identity two species’ oils and the relationship between variation of fatty acid composition and environmental factors was analyzed. Two species seed oils contained C15-C20 fatty acids, of which palmitoleic acid was the most abundant in Z. armatum and oleic acid was the most abundant in Z. bungeanum. The built classification model showed 100% accuracy and explained proportions of total fatty acid composition’s variance by environmental factors between Z. armatum and Z. bungeanum were 83.15% and 67.43% respectively. Among all environmental factors, latitude had a significant contribution to variation of fatty acid composition in Z. armatum seed oil, while annual maximum precipitation greatly contributed to variation of fatty acid composition of Z. bungeanum seed oil.

Lipid content and fatty acid profile of selected halophytic plants reveal a promising source of renewable energy

Recent research has focused on the production of biofuel from different bio-resources, especially non-edible and underutilized plants. In this study, total lipid contents and fatty acids composition were evaluated both qualitatively and quantitatively for twelve abundant halophytes to ascertain their potential as feedstock for renewable energy. Non-succulent halophytes contained the highest total lipid content (about 5–7%, except Porteresia coarctata), followed by shrubby (about 2.6%) and succulent halophytes (1–1.8%). The FA profile shows that halophytes are a rich source of alpha-linolenic, linolenic and palmitic acids. Oleic and stearic acids were also detected in some halophytes. A low content of MUFA (except P. coarctata) was detected in all the halophytes, but the maximum content of SFA was estimated in Salicornia brachiata, Suaeda fruticosa, and P. coarctata. Halophyte Sporobolus virginicus contained more than 80% PUFA, followed by Heleochloa setulosa, Sesuvium portulacastrum, Atriplex griffithii and Salvadora persica. An important qualitative indicator density was found to be at par with recommended standards, but the cetane number of S. fruticosa and S. brachiata also matched the standards. The overall fatty acid profile of the selected halophytes makes them suitable for biofuel, and their potential could be enriched by improving agronomical practices and downstream processing.

Fixed oil from seeds of narrow-leaved ash (F. angustifolia subsp. angustifolia): Chemical profile, antioxidant and antiproliferative activities

Fraxinus angustifolia subsp. angustifolia is a plant with an age-old use for the production of manna. However, it is also a valuable source of fixed oil rich-seeds. In the present study we examined the chemical and biological properties of this oil in order to support a possible application in foodstuffs, nutraceuticals and cosmetics. Fatty acid composition, volatile and phenolic substances were evaluated. Oleic and linoleic acid represented 45.5% and 50.0%, respectively, of the total fatty acid composition. Among polar phenolic substances identified (secoiridoids, phenylethanoid glycosides, phenolic acids and alcohols, flavonoids, coumarins) isoverbascoside is for the first time reported in this species. Volatiles were mainly characterized by sesquiterpenes. The oil showed good antioxidant activity, in terms of ABTS radical scavenging activity, with an IC50 value of 28.2 μg/mL. The antiproliferative activity was also investigated: amelanotic melanoma (C32) and lung carcinoma (A549) cells were the most sensitive with IC50 values comparable to that of the positive control vinblastine. These findings shed light on the potential use of F. angustifolia subsp. angustifolia fixed oil in nutraceutics and cosmetics.

Varietal Authentication of Extra Virgin Olive Oils by Triacylglycerols and Volatiles Analysis.

In recent years, there is an increasing interest in high-quality extra virgin olive oils (EVOOs) produced from local cultivars. They have particular chemical/organoleptic characteristics and are frequently subjected to fraud, whereby the control of quality requires a powerful varietal check. In the present research, triacylglycerols (TAGs) and volatiles have been studied as chemical markers for the authentication of EVOO samples from four Italian varieties of Olea europea (Dolce Agogia, Frantoio, Leccino, and Moraiolo). The monocultivar EVOO samples have been subjected to a chemical–enzymatic chromatographic method in order to perform a stereospecific analysis, an important procedure for the characterization of TAG of food products. The results, combined with chemometric analysis (linear discriminant analysis, LDA), were elaborated in order to classify Italian EVOO monocultivar samples. In accordance with the total and intrapositional fatty acid (FA) composition of TAG fraction, the results were allowed to carry out a varietal discrimination. In addition, volatile compounds were also determined by solid-phase micro-extraction gas chromatography-mass spectrometry analysis. All EVOO samples were correctly classified when TAG stereospecific data and volatile results were elaborated by the LDA procedure, even if volatile compounds showed a higher discriminant power.

Seasonal variations in the total lipid content and fatty acid composition of cultured and wild Crassostrea madrasensis in Sri Lanka

The marketplace contains a choice of both cultured and wild oysters, often subtle differences in taste and characteristics are observed between wild and cultured oysters. Therefore, seasonal variations of total lipid and total fatty acid compositions were studied in both, wild and cultured populations of Crassostrea madrasensis in Gangewadiya in Kala-Oya estuary and in cultured populations in the Puttalam lagoon in Sri Lanka over a 13 month period. The fatty acid profile was analyzed using Gas Chromatography. The average lipid percentage was 1.28 ± 0.02% and there was no significant difference between cultured and wild oysters (p > 0.05). A total of 17 fatty acids was detected. Significant monthly variations were seen in all fatty acids, except docosapentaenoic acid (DPA/C22.5(n-3)) and docosahexaenoic acid C226(n-3). Significantly high concentrations (p < 0.05) of omega 3 fatty acids were recorded during October while significantly higher omega 6 concentrations were recorded during April (p > 0.05). Saturated fatty acids had the highest concentration followed by polyunsaturated fatty acids and monounsaturated fatty acids. Palmitic acid was the major saturated fatty acid and docosahexaenoic acid was the major polyunsaturated fatty acid. The ratio of total n-3 PUFA to total n-6 PUFA were 2.6, 2.9 and 3.0 in Gangewadiya wild, Gangewadiya cultured and Kandakuliya cultured oysters, respectively. Though there were no significant differences in omega 3 to omega 6 ratios in cultured and wild oysters in Gangewadiya, a significantly higher ratio was calculated for cultured oysters in Kandakuliya. Hence, a discriminant analysis indicated that in terms of the fatty acid composition, cultured C. madrasensis in Kandakuliya is distinct compared to wild and cultured populations from Gangewadiya. Since, both wild and cultured C. madrasensis shared the same waters in Gangewadiya, the lack of differences can be explained. However, in both sites and under wild and cultured conditions, fatty acid ratio confirms the importance of C. madrasensis as an ideal source of omega 3.

First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms.

The goal of this research was a comprehensive analysis of four wild edible mushroom species, Cantharellus cinereus, Clavariadelphus pistillaris, Clitocybe nebularis and Hygrocybe punicea, which have not been analyzed so far. Extracts of different polarities have been prepared and evaluated for their antioxidant activities by DPPH, ABTS, FRAP, TRP and CUPRAC methods. For all extracts, total phenolic content was determined. Based on the analysis, it was shown that solvent type had a significant effect on the antioxidant capacities of mushroom extracts, so water extracts showed the highest activity. Furthermore, the analysis includes determination of mineral composition, fatty acid profiles and antimicrobial activity. Unsaturated fatty acids, which are very important for human health, are dominant in the studied mushroom species. Linoleic and oleic acid consist of over 50 % of the total fatty acid composition. Seventeen biologically important and toxic elements have been analyzed by ICP-OES and ICP-MS and results showed that the element concentrations were species-dependent. Also, it has been found that analyzed mushrooms did not show any antimicrobial activity. Chemometric analysis was used to understand the connection between the extracts of different polarities.

Sex and seasonal variation in proximate composition and fatty acid profile of &lt;/em&gt;Scomber scombrus&lt;/em&gt; (L. 1758) fillets from the Middle East Coast of Tunisia

This study investigates the impact of season and sex variations on the total lipid contents and fatty acid composition of Scomber scombrus fillets from the Middle East Coast of Tunisia in order to dertermine the most favorable periods for consumption, and to see if the nutritional quality of the meat depends on the sex of the animal. The effect of fishing season induced significant changes in the lipid profile, and the highest values for total lipids were obtained in the spring for females with 13.2% and for males with 18.9%. The highest values for proteins were obtained in the summer for females with 22.0% and for males with 21.8%. Protein content variations were not significant (p &gt; 0.05). The n-3/n-6 ratio showed a significant level, indicating a tendency toward n-3 fatty acid accumulation in mackerel fillets mainly represented by DHA, whose values were high during the study period for both sexes, except in autumun, which is the period of mackerel gonad maturation, when DHA decreased significantly (p &lt; 0.05), reaching 23.2 and 34.0% for males and females, respectively. It was concluded that Scomber scombrus has high levels of proteins, lipids, and fatty acid contents mainly n-3 PUFA. DHA sex variations were not significant (p &gt; 0.05). The Atherogenicity index (AI) and Thrombogenicity index (TI) were calculated. In our study the AI index was comprised between 0.3 and 0.6 for males and between 0.4 and 0.5 for females. The TI index values ranged from 0.6 to 0.8 for males, and was about 0.6 for females.

First seasonal investigation of the fatty acid composition in three organs of the Tunisian bivalve &lt;em&gt;Mactra stultorum&lt;/em&gt;

This study reveals information for the first time about the total lipid (TL) content and fatty acid composition (FA) of Mactra stultorum (M. corallina). Three edible organs (foot, mantle and adductor muscle) were the subjects of this research in order to determine the most favorable periods for their consumption in relation to seasonal variability. The results showed lower lipid content in the adductor muscles in summer (12.73 ± 2.55 mg/g dry matter); while a higher content was observed in winter (28.97 ± 3.50 mg/g dry matter). However, similar lipid contents were observed in the mantle and foot tissues among the seasons. The fatty acid composition of Mactra stultorum adductor muscles, mantle and foot was dominated by saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA). Palmitic acid (16:0) was the major saturated fatty acid (SFA) and reached higher levels during summer in the foot (26%), mantle (21%) and adductor muscles (25%). Among PUFA, eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) showed significant variation among seasons with high levels recorded during winter and spring. Monounsaturated fatty acids (MUFA) did not show any remarkable variation among seasons for the three studied tissues. Seasonal changes in fatty acids were observed for all samples, reaching a maximum level in winter or spring.

Associations of plasma total phospholipid fatty acid patterns with feeding practices, growth, and psychomotor development in 6-month-old South African infants.

The objective of this study was to assess plasma fatty acid (FA) patterns of 6-month-old South African infants and to determine their association with feeding practices, growth, and psychomotor development. Plasma total phospholipid FA composition (% of total FAs) of 6-month-old infants (n=353) from a peri-urban township was analysed, and principal component and factor analysis were performed to identify plasma FA patterns. Feeding practices, anthropometric measurements, and psychomotor development scores were determined. Four major plasma phospholipid FA patterns were identified: A plant-based C18 FA, a high n-6 long-chain polyunsaturated fatty acids (LCPUFA), a C16:1 and long-chain saturated fatty acid (SFA), and a high n-3 and low n-6 LCPUFA pattern. Formula feeding was associated with higher, whereas breastfeeding was associated with lower scores for the plant-based C18 FA and C16:1 and long-chain SFA patterns. On the other hand, breastfeeding, the consumption of cow's milk, and the consumption of semisolid foods were associated with higher scores, whereas formula feeding was associated with lower scores for the high n-6 LCPUFA pattern. Breastfeeding and the consumption of semisolids were also associated with higher high n-3 and low n-6 LCPUFA pattern scores. The C16:1 and long-chain SFA and high n-3 and low n-6 LCPUFA patterns were positively associated with psychomotor development scores. In 6-month-old South African infants, we identified distinct plasma FA patterns that presumably represent the FA quality of their diet and that are associated with psychomotor development. Our results suggest that breast milk is an important source of n-6 LCPUFAs and formula-fed infants may be at risk of inadequate LCPUFA intake.

Correction to: Testing the dogma that total phospholipid fatty acid composition of blood plays a role in kidney stone pathogenesis, using a high-low risk human model: results from a pilot study.

The authors would like to add the following paragraph in the acknowledgement section of the original version of the paper.

Impact of Purslane (Portulaca oleracea L.) Leaves Extract to Enhance the Anti-oxidant Potential of Edible Oils during Heating.

The present work deals/ reveals with the effect of purslane leaves extract on the stability of soybean oil during heating and the acceptability of oil after preparation of poori (an Indian fried bread) by frying and its sensory evaluation. The ethanolic purslane leaves extract was blended with soybean oil at three different concentrations such as, 500, 1000 and 1500 ppm (T1, T2 and T3) and compared with control. The sample added with 100 ppm TBHQ was used as a positive control. Assessment of antioxidant activity of the ethanolic extract of purslane leaves was carried out by the estimation of total phenolic content, loss of β-carotene and antioxidant activity. The heating (173±2°C for 24 h; 8 h heating cycles per day) performance of soybean oil incorporated with purslane leaves extract was evaluated in terms of peroxide value, free fatty acid, total polar material and fatty acid composition. The thermal stability of the oils was evaluated using differential scanning calorimeter. The poori was prepared to check the acceptability of the oil. Results suggest that leaves extract of purslane (1500 ppm) may be used for obtaining reasonable thermal stability of soybean oil with acceptable sensory characteristics of the product. Although TBHQ showed almost similar thermal stability with leaves extract of purslane (1500 ppm), natural anti-oxidant is more preferred over synthetic anti-oxidant.

Lipid composition and DPPH activities of the seed oil of five Turkish hazelnut genotypes (Corylus colurna L.)

Five genotypes of Turkish hazelnuts ( Corylus colurna L.) collected during the 2016 crop were analyzed for chemical composition, including total oil content, fatty acid and sterol composition. The oil yields from these kernels varied from 36.5% to 60.8%. The main fatty acids of Turkish hazelnuts kernel oils were oleic acid (79.34–83.0%) and linoleic acid (7.52–10.81%). The polyunsaturated/saturated fatty acid ratio is low, ranging from 0.87 to 1.25. Total phytosterol content ranged from 4.52 to 6.50 mg g -1 of oil. Among the eleven sterols identified and quantified, s -sitosterol was the major one with a mean percentage of 65.09%, while fucosterol and campesterol was the second and the third components of the group with mean values of 10.91 and 4.36% respectively. The antioxidant potential was evaluated by radical scavenging activity using 2.2-diphenyl-1 picrylhydrazyl (DPPH) assay.