Topical Anti-inflammatory Activity Research Articles

Phytochemistry and Evaluation of the Anti-Inflammatory Activity of the Hydroethanolic Extract of Virola elongata (Benth.) Warb. Stem Bark.

Previous studies of the hydroethanolic extract of Virola elongata inner stem bark (HEVe) have demonstrated its antioxidant, gastroprotective, and antiulcer properties, but have not evaluated its anti-inflammatory potential. HEVe was obtained by maceration and phytochemically analyzed. Its systemic anti-inflammatory activity was assessed by its effect on lipopolysaccharide (LPS)-induced peritonitis in mice. HEVe gel (HEgVe) was employed to evaluate topical anti-inflammatory activity by measuring the ear edema resulting from croton-oil-induced dermatitis in mice. A cell viability assay was conducted to determine the non-cytotoxic concentrations of the HEVe. RAW 264.7 cells were stimulated by LPS to determinate cytokine and nitric oxide production. A phytochemical analysis of the HEVe revealed the presence of phenolic acids, neolignans, flavonoids, and monomeric catechins. The oral treatment of acute peritonitis with HEVe reduced the total leukocytes, neutrophils, TNF-α, and IL-1β and elevated IL-10 levels. The application of the HEgVe reduced local edema. The HEVe on the RAW 264.7 cells exhibited no cytotoxicity, and the cells with HEVe displayed reduced TNF-α, IL-1β, and NO levels and increased IL-13 levels. HEVe demonstrated systemic and topical multitarget anti-inflammatory activity, likely due to the combined effects of secondary metabolites. HEVe emerges as a promising herbal remedy for inflammation with minimal cytotoxicity, emphasizing its potential therapeutic significance.

Anti-inflammatory efficacy and skin irritation study of Cleome gynandra extract: an animal model and pre-clinical evaluation

Cleome gynandra, a kind of medicinal herb which has strong antioxidant properties has been traditionally used for treating various ailments including skin inflammatory diseases. To date, the anti-inflammatory efficacy of C. gynandra based cream has not been reported. The aim of the study was to evaluate the anti-inflammatory activities of C. gynandra leaf extract cream using an in vivo arachidonic acid-induced ear edema in the rat model. The expression of genes related to rat ear inflammation was measured to evaluate the topical anti-inflammatory activity of C. gynandra cream. The toxicity effect of C. gynandra cream was determined through a skin irritation test in rats and human cumulative skin irritation assessment. Data collected evidently showed a significantly higher (p<0.05) percentage of recovery rate (70.21±5.14%) in rats treated with C. gynandra cream when compared to the negative control (27.43±4.75%) and basal cream (55.8±4.61%), indicating that C. gynandra cream effectively reduced inflammation symptoms in rat ear edema model study. The expressions of all pro-inflammatory cytokines and acute inflammatory-related genes were significantly down-regulated in C. gynandra cream-treated group than untreated group (negative control). The dermatological safety aspect of C. gynandra cream was confirmed by human cumulative skin irritation assessment with the average cumulative irritation index of 0.046, indicating a non-irritating effect. The overall findings demonstrated that C. gynandra cream has great potential to be applied for treating skin-related inflammatory diseases.

Острый тонзиллофарингит: возможности комбинированного лечения

Acute sore throat is a prevalent concern among patients visiting medical specialists and pharmacies alike. The primary etiology is commonly a combination of acute tonsillitis, characterized by inflammation of the palatine tonsils, and acute pharyngitis, involving inflammation of the posterior and lateral pharyngeal walls. Typically, systemic antibiotic therapy is not necessary for managing these cases. The predominant treatment approach involves the prescription of topical antiseptics delivered through lozenges and sprays. Each formulation, along with its active constituents, has specific attributes that must be considered during treatment tactics. Topical antiseptic therapy offers several benefits, including direct efficacy at the site of inflammation, minimal systemic absorption, and a low potential for developing microbial resistance. A rational therapeutic strategy involves using a combination of ambazone lozenges, which exert a bacteriostatic effect against various pathogenic microorganisms responsible for acute tonsillopharyngitis, and benzydamine spray, which provides a complementary topical anti-inflammatory action to enhance ambazone's antimicrobial properties. This combination effectively targets multiple aspects of the pathological process, offering a well-rounded therapeutic advantage. The article supports the strategic implementation of this combination therapy KEYWORDS: sore throat, pharyngitis, tonsillopharyngitis, ambazone, benzydamine, topical therapy. FOR CITATION: Filippova O.V. Acute tonsillopharyngitis: possibilities of combined treatment. Russian Medical Inquiry. 2024;8(8):477–483 (in Russ.). DOI: 10.32364/2587-6821-2024-8-8-6.

Isothiocyanate-rich moringa seed extract reduces skin inflammation in mouse ear edema model

BackgroundMoringa (Moringa oleifera Lam.) seed extract (MSE) and its primary bioactive compound, moringa isothiocyanate-1(MIC-1), mitigate inflammation, oxidative stress, diabetes, and cancer in the in vivo rodent models following oral application. PurposeTo investigate the topical anti-inflammatory activity of MSE and purified MIC-1 in a TPA-induced mouse ear edema model. Study DesignThe present study elucidates the topical anti-inflammatory effects and mechanisms of action of MSE, containing 38% of MIC-1 and purified MIC-1 using a mouse ear edema model utilizing 12-O-tetradecanoylphorbol-13-acetate (TPA), as the pro-inflammatory agent. MethodsA time-dependent and dose-dependent response was determined by pretreating CD-1 mice with various doses of MSE and MIC-1, positive control, dexamethasone, or vehicle control, followed by TPA, and the subsequent difference in ear thickness was measured using digital Vernier calipers. The effective doses of MSE and MIC-1were then selected to evaluate the change in weight of the ears using 6 mm biopsy punches and the results were confirmed by microscopy. Inflammatory markers were quantified with Luminex multiplex immunoassay. ResultsMSE and MIC-1 were effective in a dose-dependent manner in a TPA-induced ear edema model, causing a reduction in ear thickness and a 48% and 49% decrease in ear punch weight, respectively. MSE and MIC-1 also caused a reduction in the levels of cytokine and chemokines, interleukin 6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and keratinocyte chemoattractant (KC) in the ear tissue. MSE and MIC-1 reduced IL-6 expression by 84% and 78%, MCP1 by 74% and 73%, and KC by 56% and 43%, respectively. Additionally, the anti-inflammatory effect of MSE and MIC-1 was confirmed by hematoxylin and eosin (H&E) staining, used to assess the thickness of the ear swelling. MSE significantly reduced the thickness of the ears by 20% compared to TPA. ConclusionThese results reveal the topical anti-inflammatory properties of MSE, and MIC-1 likely transmitted via the nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor-kappa B (NF-κB) pathways as mentioned in previous studies. This work also suggests therapeutic uses of MSE and/or MIC-1 for skin inflammation.

A CRITICAL REVIEW AND SIGNIFICANCE OF LIPID-BASED AYURVEDIC DOSAGE FORMS GHRITA AND TAILA: PART I-REVIEW AND STATUS

This review discusses in detail one of the oldest lipid-based dosage form, Sneha Kalpana as described in Ayurved, one of the ancient medical systems. Here the vital constituents of plants are extracted as assimilates in lipid forms, ghrit–the clarified butter or taila–the oil. These dosage forms are administered through different routes for specific therapeutic benefits varying from topical nourishment, anti-inflammatory activity, and healing of tissue systems or even to treat serious neuro-muscular or neurological conditions. This review highlights the basic concepts of Ayurved behind the drug delivery approach and provides details of different processes of preparation or manufacture. Lipid-based drug delivery systems (LBDDS) are one of the emerging technologies designed to address the challenges of solubility and bioavailability of poorly water-soluble ingredients. Understanding the principles behind the processes–the right amount of heat, time, and intensity is important for standardization to ensure the availability of bio constituents. Also, it is essential to understand the relevance of therapeutic principles behind these dosage forms to choose an appropriate technological adaptation for its optimum clinical effectiveness.

Antiinflammatory Activity of Polyherbal Gel Formulation

The present study has been undertaken with the aim to formulate and evaluate the new polyherbal gel formulation containing 0.3 % hydroalcoholic extract from Trigonellafoenum-graecum and Glycyrrhiza glabra for its topical anti inflammatory activity against carrageenan induced rat paw edema method .The studies were conducted on Wistar rats of either sex (150-180g).The polyherbal gel formulation was designed by using Carbopol 934, SodiumCMC, Trigonellafoenum-graecum, Glycyrrhizaglabra extract, Ethanol, propylene glycol 400, methyl paraben, propyl paraben, and required amount of distilled water. The skin ph (6.8-7) was maintained by drop wise addition of tri- ethanolamine. The prepared polyherbal gels were evaluated for physical appearance, pH, spreadability, skin irritation to observe side effect. It was inferred from the results that polyherbal gel formulations were good in appearance and homogeneity. The overall result of this research the prepared poly herbal gel formulation shows significant anti-inflammatory activity.

Formulation and Pharmacological Evaluation of Herbal Gel Containing Curcuma longa

The aim of the present study is to formulate and evaluate a polyherbal gel that contains Curcuma longa extract. The ethanolic extract of C. longa’s rhizome was used to create a gel formulation in different concentrations (1, 2, 3, and 4%). Topical anti-inflammatory activity of gel was also assessed. Gel was prepared using Carbopol® 940 (1% w/v), C. longa extract, ethanol, propylene glycol 400, methyl paraben, propyl paraben, ethylenediaminetetraacetic acid, tri-ethanolamine, and the necessary amount of distilled water. The prepared formulations were assessed for their physical qualities, pH, spreadability, and ability to irritate skin to detect toxicity or side effects. The findings suggested that gel compositions were good in terms of appearance and uniformity.

Design and Characterization of Lipid-Surfactant-Based Systems for Enhancing Topical Anti-Inflammatory Activity of Ursolic Acid.

Skin inflammation is a symptom of many skin diseases, such as eczema, psoriasis, and dermatitis, which cause rashes, redness, heat, or blistering. The use of natural products with anti-inflammatory properties has gained importance in treating these symptoms. Ursolic acid (UA), a promising natural compound that is used to treat skin diseases, exhibits low aqueous solubility, resulting in poor absorption and low bioavailability. Designing topical formulations focuses on providing adequate delivery via application to the skin surface. The aim of this study was to formulate and characterize lipid-surfactant-based systems for the delivery of UA. Microemulsions and liquid crystalline systems (LCs) were characterized by polarized light microscopy (PLM), rheology techniques, and textural and bioadhesive assays. PLM supported the self-assembly of these systems and elucidated their formation. Rheologic examination revealed pseudoplastic and thixotropic behavior appropriate, and assays confirmed the ability of these formulations to adhere to the skin. In vivo studies were performed, and inflammation induced by croton oil was assessed for response to microemulsions and LCs. UA anti-inflammatory activities of ~60% and 50% were demonstrated by two microemulsions and 40% and 35% by two LCs, respectively. These data support the continued development of colloidal systems to deliver UA to ameliorate skin inflammation.

Baccharis dracunculifolia DC (Asteraceae) Root Extract and Its Triterpene Baccharis Oxide Display Topical Anti-Inflammatory Effects on Different Mice Ear Edema Models.

B. dracunculifolia is popularly used to treat skin diseases. This work aimed to evaluate the topical anti-inflammatory properties of B. dracunculifolia root extract (BdR) and its major compound baccharis oxide (BOx) on mice ear edema models. BdR was analyzed by GC-MS, and BOx was isolated by chromatographic fractionation. Topical anti-inflammatory activities were determined by using the croton oil, capsaicin, histamine, and phenol-induced mouse ear edema models. N-acetyl-β-D- glucosaminidase (NAG) and myeloperoxidase (MPO) activities, as well as NO dosage and histopathological analyses, were also evaluated. Phytochemical analysis of BdR showed BOx as one of the major constituents. BdR and BOx (both at 0.1, 0.5, and 1.0 mg/ear) significantly reduced croton oil, histamine, and phenol-induced ear edema, while only BOx was effective in reducing capsaicin-induced edema. MPO and NAG activities, as well as NO production, were significantly inhibited by BdR and BOx. Histopathological analysis confirmed the topical anti-inflammatory properties of BdR and BOx. Our findings showed that BdR and BOx demonstrated significant topical anti-inflammatory effects in mouse ear edema induced by different agents, suggesting their possible application on skin inflammatory diseases.

Topical anti-inflammatory and analgesic activities of Laportea decumana (Roxb) Wedd extract cream in rats

Topical anti-inflammatory and analgesic activities of Laportea decumana (Roxb) Wedd extract cream in rats

Non-carcinogenic/non-nephrotoxic aristolochic acid IVa exhibited anti-inflammatory activities in mice.

Aristolochic acid (AA)-containing herbs have been prescribed for thousands of years as anti-inflammatory drugs, despite the active pharmaceutical ingredients remaining unclear. However, exposure to AAI and AAII has been proven to be a significant risk factor for severe nephropathy and carcinogenicity. AAIVa, an analogue abundant in AA-containing herbs, showed neither carcinogenicity nor nephrotoxicity in our study and other reports, implying that the pharmacological effects of AAIVa on inflammation are worth studying. Herein, we employed RAW 264.7 cells, the ear edema mouse model, and the lipopolysaccharide (LPS)-induced systematic inflammation model in TNF-IRES-Luc mice (tracking TNFα luciferase activities in real-time) to evaluate the anti-inframammary effect of AAIVa. Our results showed that AAIVa could decrease pro-inflammatory cytokines (TNFα and IL-6) production in LPS-stimulated RAW 264.7 cells, indicating its anti-inflammatory effects in vitro. Furthermore, the application of AAIVa (400 and 600μg/ear) could significantly inhibit phorbol 12-myristate 13-acetate-induced ear edema, suggesting its topical anti-inflammatory activity in vivo. Moreover, LPS-stimulated TNF-IRES-Luc mice were used to investigate the onset and duration of AAIVa on systematic inflammation. A single dosage of AAIVa (100mg/kg, i.g.) could suppress LPS-triggered inflammation, by decreasing luciferase activities of TNFα at 3h in TNF-IRES-Luc mice. In addition, the online pharmacological databases predicted that AAIVa might target the regulation of T cell activation-related protein (ADA, ADORA2A, ERBB2) to exhibit anti-inflammatory effect. In conclusion, we demonstrated that AAIVa had anti-inflammatory effect for the first time; our findings are constructive for further studies on pharmacological mechanism of AAIVa.

Hyaluronic Acid Micelles for Promoting the Skin Permeation and Deposition of Curcumin.

BackgroundThe poor skin permeation and deposition of topical therapeutic drugs is a major issue in topical drug delivery, improving this issue is conducive to improving the topical therapeutic effect of drugs.MethodsIn this study, octadecylamine modified hyaluronic acid (OHA) copolymer was synthesized by amide reaction technique to prepare curcumin (CUR)-loaded micelles (CUR-M) for topical transdermal administration. CUR-M was successfully prepared by dialysis, and the formulation was evaluated for particle size, zeta potential, surface morphology, entrapment effciency (EE%), drug loading (DL), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and the in vitro drug release. Additionally, in vitro skin permeation and retention, in vivo topical analgesic and anti-inflammatory activity, and skin irritation were assessed.ResultsThe mean drug loading (DL), drug entrapment efficiency (EE), hydrodynamic diameter and zeta potential of CUR-M were 8.26%, 90.86%, 165.64 nm and −26.85 mV, respectively. CUR-M was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), it was found that there was an interaction between CUR and OHA, and CUR existed in CUR-M in an amorphous form. CUR-M exhibited sustained release in 48 h and good stability at 4 °C for 21days. CUR-M could significantly increase the skin penetration and retention of CUR and had better analgesic and anti-inflammatory activities in vivo when compared with CUR solution. Hematoxylin-eosin staining results revealed that the transdermal penetration mechanism of CUR-M might be related to the hydration of stratum corneum by HA. In addition, CUR-M showed no skin irritation to mouse skin.ConclusionCUR-M might be a promising and safe drug delivery system for the treatment of topical diseases.

Pentapeptide modified ethosomes for enhanced skin retention and topical efficacy activity of indomethacin

Challenges associated with topical analgesics and anti-inflammatory drugs include poor drug penetration and retention at the desired lesion site. Therefore, improving these challenges would help to reduce the toxic and side effects caused by drug absorption into the systemic circulation and improve the therapeutic efficacy of topical therapeutic drugs. Pentapeptide (KTTKS) is a signal peptide in skin tissue, it can be recognized and bound by signal recognition particles. In the current study, we successfully prepared novel indomethacin (IMC) loaded KTTKS-modified ethosomes (IMC-KTTKS-Es), and the physicochemical properties and topical efficacy were investigated. Results showed that the prepared IMC-KTTKS-Es displayed a particle size of about 244 nm, a negative charge, good deformability, and encapsulation efficiency (EE) exceeding 80% for IMC, with a sustained release pattern. In vitro percutaneous permeation studies revealed that the skin retention was increased after the drug was loaded in the IMC-KTTKS-Es. Confocal laser scanning microscopy also showed improved skin retention of IMC-KTTKS-Es. In addition, IMC-KTTKS-Es showed improved topical analgesic and anti-inflammatory activity with no potentially hazardous skin irritation. This study suggested that the IMC-KTTKS-Es might be an effective drug carrier for topical skin therapy with a good safety profile.

Anti-Inflammatory Activity of Alpinia malaccensis (Burm. f.) Roscoe and Kaempferia galanga L. Rhizome Essential Oil Gel Formulations by Carrageenan Induction Method

Bioactive compound from essential oils of Alpinia malaccensis (AM) and Kaempferia galanga (KG) rhizomes, respectively, such as methyl cinnamate and ethyl-p-methoxycinnamate (EPMS) have been reported to have anti-inflammatory activity. The present study aimed to determine the topical anti-inflammatory activity of 3 gel formulations (Formula 1, 2 and 3) having different concentrations of essential oil from AM and KG rhizomes. The gelling agent used in this study was Carbopol 934. The physicochemical parameters of the gel formulations such as organoleptic, pH, viscosity, and spreadability as well as hedonic test were also examined. The anti-inflammatory activity assay was carried out using the carrageenan induced rat paws edema method. The chemical composition of AM and KG rhizomes was identified by GC-MS with LRI index. The major essential oils content in AM and KG rhizome was methyl cinnamate (58.80%) and ethyl-p-methoxy cinnamate (59.68%), respectively. Based on the results of physical evaluation, hedonic test and anti-inflammatory activity, Formula 1 with 5% AM and 2.5% KG essential oils is the best formula with % inflammation inhibition of 36.32 ± 6.32%.

Correction to: Topical Anti-Inflammatory Activity of Petiveria alliacea, Chemical Profiling and Computational Investigation of Phytoconstituents Identified from its Active Fraction

Correction to: Topical Anti-Inflammatory Activity of Petiveria alliacea, Chemical Profiling and Computational Investigation of Phytoconstituents Identified from its Active Fraction

The Nigerian Chikadoma Plant

Introduction: A plant commonly referred to as Chikadoma in Nigeria, yellow bush in English, is traditionally used for the treatment of microbial infections and inflammation. Objectives: This study evaluated a novel gel formulation of the Nigerian Chikadoma plant extract for its topical antimicrobial and anti-inflammatory activities against pure clinical isolates (Bacillus subtilis, Salmonella paratyphi, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger), and carrageenan-induced edema on experimental rodents, respectively. Materials and Methods: Concentration of 1% w/w Carbopol-934 was employed as the gelling agent. The physiochemical parameters of formulations (viscosity, pH, spreadability, extrudability, and stability) were examined for a month to ensure that when applied on the skin, it never produced any skin irritation/erythema/edema. Results: The physiochemical and stability assessment of the gel formulation showed viscosity, pH, spreadability, extrudability, and stability within standard specifications. The antimicrobial studies showed activity against some of the test microorganisms, while the carrageenan-induced edema was inhibited. Both showed high degree of reproducibility. Conclusion: The formulated gel of Nigerian Chikadoma plant extract significantly possesses herbal anti-inflammatory and antimicrobial effects, and the gel preparation was stable under normal storage environment and did not manifest skin irritation/erythema and edema when applied on the skin.

Topical Anti-Inflammatory Activity of Petiveria alliacea, Chemical Profiling and Computational Investigation of Phytoconstituents Identified from its Active Fraction

Topical Anti-Inflammatory Activity of Petiveria alliacea, Chemical Profiling and Computational Investigation of Phytoconstituents Identified from its Active Fraction

Oral and Topical Anti-Inflammatory Activity of Jatropha integerrima Leaves Extract in Relation to Its Metabolite Profile.

Jatropha integerrima Jacq., family: Euphorbiaceae, is used in India and subtropical Africa to treat different skin conditions. In this study we evaluated the anti-inflammatory activity of J. integerrima leaves extract (JILE) using rat paw edema model. The extract was administered orally (200 and 400 mg/kg) or applied topically as creams at 2.5, 5, and 10% strength. Four hours post-treatment, maximum reduction of edema volume by 63.09% was observed after oral administration of JILE (400 mg/kg) as compared to indomethacin with 60.43%. The extract anti-inflammatory effect was accompanied by a decrease in NO, prostaglandin PGE2, TNF-α and PKC levels by 19, 29.35, 16.9, and 47.83%, respectively. Additionally, topical applications of JILE showed dose dependent reduction in paw edema and resulted in normalized levels of PGE2, TNF-α, and PKC when used as 10% cream. Signs of inflammations were reduced or absent from paw tissue of animals receiving JILE either orally or topically. Finally, liquid chromatography/mass spectrometry analysis of JILE resulted in the annotation of 133 metabolites including 24 diterpenoids, 19 flavonoids, 10 phenolic acid conjugates, 8 cyclic peptides, 6 phytosterols, 4 sesquiterpenes, and 4 coumarins. Several of the annotated metabolites have known anti-inflammatory activity including vitexin, isovitexin, fraxitin, scopeltin, stigmasterol, and many diterpenoidal derivatives.

Application of Natural Products in Radiotherapy-Induced Dermatitis: A Comprehensive Review

Radiodermatitis (RD) is experienced by many cancer patients ‎receiving radiotherapy. An increasing number of these patients demand alternative natural therapies. This study aimed to review the natural products application in cancer patients who experience RD.‎ A search of studies published from 1990 to ‎‎2020 in the ‎databases including PubMed, Scopus, and Google Scholar was performed with the keywords relevant to “Radiotherapy” “Dermatitis” and “Natural Products”. Out of 73 papers obtained, 40 papers were excluded which described only protocols or were non-clinical, non-English language, or without full text. The obtained studies were ‎discussed in detail according to the outcomes and potential mechanisms of action for each natural ‎product.‎ Clinically studied natural products were found to show several outcomes from non-effective to effective in diminishing various items of RD. Outcomes on the effectiveness of Aloe vera were diverse. Some trials suggest that Silybum marianum, Boswellia, Nigella sativa, olive oil, Lianbai, and Hypericum perforatum as well as some multi-ingredient products might be effective prophylactic treatments for RD. Potential mechanisms of these natural products included topical hydrating, anti-inflammatory, antioxidant, and wound ‎healing activities. Results from this review shows that there are some promising natural product options for the prevention and treatment of RD via their multifactorial bioactivities. However, additional research is needed before any definitive conclusions. A larger sample size, optimum doses and duration of intervention as well as investigation of treatment effects in diverse populations and comorbid complications would also be essential in future studies.

Topical anti-inflammatory effect of hydroalcoholic extract of leaves of Licania rigida Benth. in mice

BackgroundThe use of medicinal plants is one of the oldest practices of humanity, its use is aimed at the treatment, cure and prevention of diseases. The plant Licania rigida Benth., popularly known as icticic, is used in folk medicine to treat flu, diarrhea, epilepsy and malaria, having some scientifically proven biological activities, such as its antioxidant, antimicrobial, bactericidal and systemic anti-inflammatory action. MethodsThus, the present study aims to evaluate the topical anti-inflammatory activity of the hydroalcoholic extract obtained from the L. rigida leaves. ResultsA possible topical anti-inflammatory action was observed in screening models such as acute and chronic ear edema. In addition, in the protocols for acute inflammation, the extract had an anti-hematogenic potential. ConclusionsThe results are promising and can be a useful tool for more specific and effective studies on the anti-inflammatory effect in the construction of future drugs for improvement in cases of inflammation.