In the context of topcolor-assisted technicolor (TC2) models, we calculate the associated production of the neutral top-pion $\pi_{t}^{0}$ with a pair of top quarks via the process $e^{+}e^{-}\longrightarrow t\bar{t}\pi_{t}^{0}$. We find that the production cross section is larger than that of the process $ e^{+}e^{-}\longrightarrow t\bar{t}H$ both in the standard model (SM) and in the minimal supersymmetric SM. With reasonable values of the parameters in TC2 models, the cross section can reach $20fb$. The neutral top-pion $\pi_{t}^{0}$ may be direct observed via this process.