Top-down Manipulations Research Articles

Morphogenesis as Bayesian inference: A variational approach to pattern formation and control in complex biological systems.

Recent advances in molecular biology such as gene editing [1], bioelectric recording and manipulation [2] and live cell microscopy using fluorescent reporters [3], [4] - especially with the advent of light-controlled protein activation through optogenetics [5] - have provided the tools to measure and manipulate molecular signaling pathways with unprecedented spatiotemporal precision. This has produced ever increasing detail about the molecular mechanisms underlying development and regeneration in biological organisms. However, an overarching concept - that can predict the emergence of form and the robust maintenance of complex anatomy - is largely missing in the field. Classic (i.e., dynamic systems and analytical mechanics) approaches such as least action principles are difficult to use when characterizing open, far-from equilibrium systems that predominate in Biology. Similar issues arise in neuroscience when trying to understand neuronal dynamics from first principles. In this (neurobiology) setting, a variational free energy principle has emerged based upon a formulation of self-organization in terms of (active) Bayesian inference. The free energy principle has recently been applied to biological self-organization beyond the neurosciences [6], [7]. For biological processes that underwrite development or regeneration, the Bayesian inference framework treats cells as information processing agents, where the driving force behind morphogenesis is the maximization of a cell's model evidence. This is realized by the appropriate expression of receptors and other signals that correspond to the cell's internal (i.e., generative) model of what type of receptors and other signals it should express. The emerging field of the free energy principle in pattern formation provides an essential quantitative formalism for understanding cellular decision-making in the context of embryogenesis, regeneration, and cancer suppression. In this paper, we derive the mathematics behind Bayesian inference - as understood in this framework - and use simulations to show that the formalism can reproduce experimental, top-down manipulations of complex morphogenesis. First, we illustrate this 'first principle' approach to morphogenesis through simulated alterations of anterior-posterior axial polarity (i.e., the induction of two heads or two tails) as in planarian regeneration. Then, we consider aberrant signaling and functional behavior of a single cell within a cellular ensemble - as a first step in carcinogenesis as false 'beliefs' about what a cell should 'sense' and 'do'. We further show that simple modifications of the inference process can cause - and rescue - mis-patterning of developmental and regenerative events without changing the implicit generative model of a cell as specified, for example, by its DNA. This formalism offers a new road map for understanding developmental change in evolution and for designing new interventions in regenerative medicine settings.

Ultra-rapid object categorization in real-world scenes with top-down manipulations.

Humans are able to achieve visual object recognition rapidly and effortlessly. Object categorization is commonly believed to be achieved by interaction between bottom-up and top-down cognitive processing. In the ultra-rapid categorization scenario where the stimuli appear briefly and response time is limited, it is assumed that a first sweep of feedforward information is sufficient to discriminate whether or not an object is present in a scene. However, whether and how feedback/top-down processing is involved in such a brief duration remains an open question. To this end, here, we would like to examine how different top-down manipulations, such as category level, category type and real-world size, interact in ultra-rapid categorization. We have constructed a dataset comprising real-world scene images with a built-in measurement of target object display size. Based on this set of images, we have measured ultra-rapid object categorization performance by human subjects. Standard feedforward computational models representing scene features and a state-of-the-art object detection model were employed for auxiliary investigation. The results showed the influences from 1) animacy (animal, vehicle, food), 2) level of abstraction (people, sport), and 3) real-world size (four target size levels) on ultra-rapid categorization processes. This had an impact to support the involvement of top-down processing when rapidly categorizing certain objects, such as sport at a fine grained level. Our work on human vs. model comparisons also shed light on possible collaboration and integration of the two that may be of interest to both experimental and computational vision researches. All the collected images and behavioral data as well as code and models are publicly available at https://osf.io/mqwjz/.

Does semantic knowledge influence event segmentation and recall of text?

Knowledge benefits episodic memory, particularly when provided before encoding (Anderson & Pichert in Journal of Verbal Learning and Verbal Behavior, 17(1), 1-12, 1978; Bransford & Johnson in Journal of Verbal Learning and Verbal Behavior, 11(6), 717-726, 1972). These benefits can occur through several encoding mechanisms, one of which may be event segmentation. Event segmentation is one's ability to parse information into meaningful units as an activity unfolds. The current experiment evaluated whether two top-down manipulations-providing context or perspective taking-influence the segmentation and memory of text. For the ambiguous texts in Experiment 1, half the participants received context in the form of a title, whereas the other half received no context. For the text in Experiment 2, half the participants read from the perspective of a burglar and the other half read from the perspective of a home buyer. In both experiments, participants read the passages, recalled the information, and then segmented the passages into meaningful units. Consistent with previous findings, participants who received context recalled more information compared with those who received no context, and participants in one perspective were more likely to recall information relevant to their perspective. Most importantly, we found that context and perspective facilitated more normative segmentation; however, the differences were small and suggest that effects of top-down processing on the segmentation of text may be modest at best. Thus, event segmentation processes that operate during text comprehension are influenced by semantic knowledge but may be more heavily driven by other factors (e.g., perceptual cues).

Bacterial community composition responds to changes in copepod abundance and alters ecosystem function in an Arctic mesocosm study

Combining a minimum food web model with Arctic microbial community dynamics, we have suggested that top-down control by copepods can affect the food web down to bacterial consumption of organic carbon. Pursuing this hypothesis further, we used the minimum model to design and analyse a mesocosm experiment, studying the effect of high (+Z) and low (-Z) copepod density on resource allocation, along an organic-C addition gradient. In the Arctic, both effects are plausible due to changes in advection patterns (affecting copepods) and meltwater inputs (affecting carbon). The model predicts a trophic cascade from copepods via ciliates to flagellates, which was confirmed experimentally. Auto- and heterotrophic flagellates affect bacterial growth rate and abundance via competition for mineral nutrients and predation, respectively. In +Z, the model predicts low bacterial abundance and activity, and little response to glucose; as opposed to clear glucose consumption effects in –Z. We observed a more resilient bacterial response to high copepods and demonstrate this was due to changes in bacterial community equitability. Species able to use glucose to improve their competitive and/or defensive properties, became predominant. The observed shift from a SAR11-to a Psychromonodaceae – dominated community suggests the latter was pivotal in this modification of ecosystem function. We argue that this group used glucose to improve its defensive or its competitive abilities (or both). Adding such flexibility in bacterial traits to the model, we show how it creates the observed resilience to top-down manipulations observed in our experiment.

Bottom-Up and Top-Down Factors Differentially Influence Stimulus Representations Across Large-Scale Attentional Networks.

Visual attention is thought to be supported by three large-scale frontoparietal networks: the frontoparietal control network (FPCN), the dorsal attention network (DAN), and the ventral attention network (VAN). The traditional view is that these networks support visual attention by biasing and evaluating sensory representations in visual cortical regions. However, recent evidence suggests that frontoparietal regions actively represent perceptual stimuli. Here, we assessed how perceptual stimuli are represented across large-scale frontoparietal and visual networks. Specifically, we tested whether representations of stimulus features across these networks are differentially sensitive to bottom-up and top-down factors. In a pair of pattern-based fMRI studies, male and female human subjects made perceptual decisions about face images that varied along two independent dimensions: gender and affect. Across studies, we interrupted bottom-up visual input using backward masks. Within studies, we manipulated which stimulus features were goal relevant (i.e., whether gender or affect was relevant) and task switching (i.e., whether the goal on the current trial matched the goal on the prior trial). We found that stimulus features could be reliably decoded from all four networks and, importantly, that subregions within each attentional network maintained coherent representations. Critically, the different attentional manipulations (interruption, goal relevance, and task switching) differentially influenced feature representations across networks. Whereas visual interruption had a relatively greater influence on representations in visual regions, goal relevance and task switching had a relatively greater influence on representations in frontoparietal networks. Therefore, large-scale brain networks can be dissociated according to how attention influences the feature representations that they maintain.SIGNIFICANCE STATEMENT Visual attention is supported by multiple frontoparietal attentional networks. However, it remains unclear how stimulus features are represented within these networks and how they are influenced by attention. Here, we assessed feature representations in four large-scale networks using a perceptual decision-making paradigm in which we manipulated top-down and bottom-up factors. We found that top-down manipulations such as goal relevance and task switching modulated feature representations in attentional networks, whereas bottom-up manipulations such as interruption of visual processing had a relatively stronger influence on feature representations in visual regions. Together, these findings indicate that attentional networks actively represent stimulus features and that representations within different large-scale networks are influenced by different forms of attention.

Bottom-up and top-down manipulations for multi-order photonic crystallinity in a graphene-oxide colloid

Dispersions of graphene oxide nanoflakes in water can be manipulated into photonic crystals that reflect the full spectrum of visible colour. Normally, dissolving nanometre-thin graphene oxide crystals in water produces brownish solutions. Jang-Kun Song from Sungkyunkwan University in South Korea and co-workers used extensive oxidation and cleaning procedures in combination with bottom-up and top-down control strategies to coax unexpected optical activity from the irregularly shaped nanoflakes. The team's synthetic procedure produced a strong electric double layer on graphene oxide surfaces that induced the particles to stack into periodic, light-reflecting structures similar to cholesteric liquid crystals. Adding salt ions to the watery dispersion allowed the photonic structures, and hence the colour reflections, to be adjusted at molecular scales. Alternatively, the researchers could ‘paint’ glass slides by using shear forces produced by stirring to direct colour output.

Commentary: Top-down and bottom-up modulation of pain-induced oscillations.

Commentary: Top-down and bottom-up modulation of pain-induced oscillations.

Supramolecular Organic Nanowires as Plasmonic Interconnects

Metallic nanostructures are able to interact with an incident electromagnetic field at subwavelength scales by plasmon resonance which involves the collective oscillation of conduction electrons localized at their surfaces. Among several possible applications of this phenomenon, the theoretical prediction is that optical circuits connecting multiple plasmonic elements will surpass classical electronic circuits at nanoscale because of their much faster light-based information processing. However, the placement and coupling of metallic elements smaller than optical wavelengths currently remain a formidable challenge by top-down manipulations. Here, we show that organic supramolecular triarylamine nanowires of ≈1 nm in diameter are able to act as plasmonic waveguides. Their self-assembly into plasmonic interconnects between arrays of gold nanoparticles leads to the bottom-up construction of basic optical nanocircuits. When the resonance modes of these metallic nanoparticles are coupled through the organic nanowires, the optical conductivity of the plasmonic layer dramatically increases from 259 to 4271 Ω(-1)·cm(-1). We explain this effect by the coupling of a hot electron/hole pair in the nanoparticle antenna with the half-filled polaronic band of the organic nanowire. We also demonstrate that the whole hybrid system can be described by using the abstraction of the lumped circuit theory, with a far field optical response which depends on the number of interconnects. Overall, our supramolecular bottom-up approach opens the possibility to implement processable, soft, and low cost organic plasmonic interconnects into a large number of applications going from sensing to metamaterials and information technologies.

Speech processing asymmetry revealed by dichotic listening and functional brain imaging

In this article, we review research in our laboratory from the last 25 to 30 years on the neuronal basis for laterality of speech perception focusing on the upper, posterior parts of the temporal lobes, and its functional and structural connections to other brain regions. We review both behavioral and brain imaging data, with a focus on dichotic listening experiments, and using a variety of imaging modalities. The data have come in most parts from healthy individuals and from studies on normally functioning brain, although we also review a few selected clinical examples. We first review and discuss the structural model for the explanation of the right-ear advantage (REA) and left hemisphere asymmetry for auditory language processing. A common theme across many studies have been our interest in the interaction between bottom-up, stimulus-driven, and top-down, instruction-driven, aspects of hemispheric asymmetry, and how perceptual factors interact with cognitive factors to shape asymmetry of auditory language information processing. In summary, our research have shown laterality for the initial processing of consonant–vowel syllables, first observed as a behavioral REA when subjects are required to report which syllable of a dichotic syllable-pair they perceive. In subsequent work we have corroborated the REA with brain imaging, and have shown that the REA is modulated through both bottom-up manipulations of stimulus properties, like sound intensity, and top-down manipulations of cognitive properties, like attention focus.

Expertise Effects in Face-Selective Areas are Robust to Clutter and Diverted Attention, but not to Competition

Expertise effects for nonface objects in face-selective brain areas may reflect stable aspects of neuronal selectivity that determine how observers perceive objects. However, bottom-up (e.g., clutter from irrelevant objects) and top-down manipulations (e.g., attentional selection) can influence activity, affecting the link between category selectivity and individual performance. We test the prediction that individual differences expressed as neural expertise effects for cars in face-selective areas are sufficiently stable to survive clutter and manipulations of attention. Additionally, behavioral work and work using event related potentials suggest that expertise effects may not survive competition; we investigate this using functional magnetic resonance imaging. Subjects varying in expertise with cars made 1-back decisions about cars, faces, and objects in displays containing one or 2 objects, with only one category attended. Univariate analyses suggest car expertise effects are robust to clutter, dampened by reducing attention to cars, but nonetheless more robust to manipulations of attention than competition. While univariate expertise effects are severely abolished by competition between cars and faces, multivariate analyses reveal new information related to car expertise. These results demonstrate that signals in face-selective areas predict expertise effects for nonface objects in a variety of conditions, although individual differences may be expressed in different dependent measures depending on task and instructions.

Ultra rapid object categorization: effects of level, animacy and context.

It is widely agreed that in object categorization bottom-up and top-down influences interact. How top-down processes affect categorization has been primarily investigated in isolation, with only one higher level process at a time being manipulated. Here, we investigate the combination of different top-down influences (by varying the level of category, the animacy and the background of the object) and their effect on rapid object categorization. Subjects participated in a two-alternative forced choice rapid categorization task, while we measured accuracy and reaction times. Subjects had to categorize objects on the superordinate, basic or subordinate level. Objects belonged to the category animal or vehicle and each object was presented on a gray, congruent (upright) or incongruent (inverted) background. The results show that each top-down manipulation impacts object categorization and that they interact strongly. The best categorization was achieved on the superordinate level, providing no advantage for basic level in rapid categorization. Categorization between vehicles was faster than between animals on the basic level and vice versa on the subordinate level. Objects in homogenous gray background (context) yielded better overall performance than objects embedded in complex scenes, an effect most prominent on the subordinate level. An inverted background had no negative effect on object categorization compared to upright scenes. These results show how different top-down manipulations, such as category level, category type and background information, are related. We discuss the implications of top-down interactions on the interpretation of categorization results.

Practicing Memory in Central American Literature by Nicole Caso (review)

Reviewed by: Practicing Memory in Central American Literature by Nicole Caso Greg C. Severyn Caso, Nicole . Practicing Memory in Central American Literature. New York: Palgrave Macmillan, 2010. 288 pp. In Practicing Memory, Nicole Caso selects a variety of "mainstream" contemporary texts from Guatemala, Nicaragua, El Salvador, Costa Rica, and Honduras that employ historical fiction to cover what poet Humberto Ak'abal describes as "the mouth of silence" (2), that is, to speak out against pervasive silences and forced acceptances imposed by official history in the isthmus. Although the chosen works assume markedly different approaches as they each seek to interject their historical perspectives, their "regenerative effect" (3) becomes [End Page 107] clear in the four sections of this study as memory is accessed from distinct physical and ideological spaces. The influence of East-West politics and North-South economics is treated in each chapter, uniting Caso's critical analyses into a cohesive argument that reveals recurring imperial and colonial legacies. As a result, Central America becomes more than just a small strip of earth bridging two land masses; it becomes a spatial metaphor "intentionally left open [to] ambiguity" (4), allowing both marginal and popular, literal and figurative issues to be explored. The first two chapters comprise part one, "The Isthmus", where East-West relations converge in Central America, making clear the links between the Cold War rhetoric of the twentieth century and the isthmus's role as "elusive object of desire" (20) for foreign powers. In chapter one, Caso argues that Ernesto Cardenal's epic poem El estrecho dudoso (1966) challenges complacent univocal representation of the past by filling in the omissions in the hegemonic historical record in an effort to escape its construct. In chapter two, Caso focuses on U.S. involvement in the region by analyzing the ideological use of language in two works that deal with the 1954 Guatemalan coup d'état: Miguel Ángel Asturias's Week-end en Guatemala (1956) and Arturo Arias's novel Después de las bombas (1979). She indicates that Asturias forcefully brandishes language in his short stories in order to expose anticommunist campaigns through the manipulation of words and propaganda to legitimize U.S. intervention. Arias, on the other hand, makes language a "site of struggle" (76) by restoring some words onto the silenced, blank pages of history. Caso points to the novel's carnivalesque and satirical style as a means to "orchestrate the many self-conscious voices of resistance" (75) elaborated within a single, literary space. Caso then begins section two, "The City," which concentrates on urban spaces as entities representing the impact of modernity in Central America. Chapter three initiates this section with a study of Asturias's El señor presidente (1946). By examining the cost of "progress" in the isthmus, Caso makes the case that this novel "captures what gets left out of historiographical discourse... through sounds, spaces, and images that fall at the margins of normative language" (107). Then, the fragmented novels Diario de una multitud (1974) by Carmen Naranjo and Manlio Argueta's Caperucita en la zona roja (1977) are contrasted in chapter four to reveal how "community" can be represented either as isolated and broken-down by external market forces, or an interrelated unit able to resist in times of need. In both novels, the push towards social awareness and sociopolitical solidarity is evident. She concludes this chapter by suggesting that these works use "fragmentary narrative styles from within the discourses that they are critiquing to interrupt their homogenizing tendencies" (142). Part three of the study, "The Nation", takes a step back, spatially speaking, to consider the totalizing narrative of Julio Escoto's Rey del Albor, Madrugada (1993) in chapter five. Caso argues that Escoto opts to use the typically hegemonic [End Page 108] style of totality to give voice to his project from the margin, able to point out "top-down manipulations from abroad" (147) when conceiving ideas that pertain to mestizaje and nationalism in Honduras. Additionally, although the novel attempts to fight homogenizing tendencies imposed on the country from the outside, the risks involved in an inward "rallying around the Honduran nation" (183) and the consequent homogenizing from within are also taken...

A 200‐year perspective on alternative stable state theory and lake management from a biomanipulated shallow lake

Multiple stressors to a shallow lake ecosystem have the ability to control the relative stability of alternative states (clear, macrophyte-dominated or turbid, algal-dominated). As a consequence, the use of remedial biomanipulations to induce trophic cascades and shift a turbid lake to a clear state is often only a temporary solution. Here we show the instability of short-term manipulations in the shallow Lake Christina (Minnesota, USA) is governed by the long-term state following a regime shift in the lake. During the modern, managed period of the lake, three top-down manipulations (fish kills) were undertaken inducing temporary (5-10 years) unstable clear-water states. Paleoecological remains of diatoms, along with proxies of primary production (total chlorophyll a and total organic carbon accumulation rate) and trophic state (total P) from sediment records clearly show a single regime shift in the lake during the early 1950s; following this shift, the functioning of the lake ecosystem is dominated by a persistent turbid state. We find that multiple stressors contributed to the regime shift. First, the lake began to eutrophy (from agricultural land use and/or increased waterfowl populations), leading to a dramatic increase in primary production. Soon after, the construction of a dam in 1936 effectively doubled the depth of the lake, compounded by increases in regional humidity; this resulted in an increase in planktivorous and benthivorous fish reducing phytoplankton grazers. These factors further conspired to increase the stability of a turbid regime during the modern managed period, such that switches to a clear-water state were inherently unstable and the lake consistently returned to a turbid state. We conclude that while top-down manipulations have had measurable impacts on the lake state, they have not been effective in providing a return to an ecosystem similar to the stable historical period. Our work offers an example of a well-studied ecosystem forced by multiple stressors into a new long-term managed period, where manipulated clear-water states are temporary, managed features.

A 200-year perspective on alternative stable state theory and lake management from a biomanipulated shallow lake

Multiple stressors to a shallow lake ecosystem have the ability to control the relative stability of alternative states (clear, macrophyte-dominated or turbid, algal-dominated). As a consequence, the use of remedial biomanipulations to induce trophic cascades and shift a turbid lake to a clear state is often only a temporary solution. Here we show the instability of short-term manipulations in the shallow Lake Christina (Minnesota, USA) is governed by the long-term state following a regime shift in the lake. During the modern, managed period of the lake, three top-down manipulations (fish kills) were undertaken inducing temporary (5–10 years) unstable clear-water states. Paleoecological remains of diatoms, along with proxies of primary production (total chlorophyll a and total organic carbon accumulation rate) and trophic state (total P) from sediment records clearly show a single regime shift in the lake during the early 1950s; following this shift, the functioning of the lake ecosystem is dominated by a persistent turbid state. We find that multiple stressors contributed to the regime shift. First, the lake began to eutrophy (from agricultural land use and/or increased waterfowl populations), leading to a dramatic increase in primary production. Soon after, the construction of a dam in 1936 effectively doubled the depth of the lake, compounded by increases in regional humidity; this resulted in an increase in planktivorous and benthivorous fish reducing phytoplankton grazers. These factors further conspired to increase the stability of a turbid regime during the modern managed period, such that switches to a clear-water state were inherently unstable and the lake consistently returned to a turbid state. We conclude that while top-down manipulations have had measurable impacts on the lake state, they have not been effective in providing a return to an ecosystem similar to the stable historical period. Our work offers an example of a well-studied ecosystem forced by multiple stressors into a new long-term managed period, where manipulated clear-water states are temporary, managed features.

Spatial Interactions in the Superior Colliculus Predict Saccade Behavior in a Neural Field Model

During natural vision, eye movements are dynamically controlled by the combinations of goal-related top-down (TD) and stimulus-related bottom-up (BU) neural signals that map onto objects or locations of interest in the visual world. In primates, both BU and TD signals converge in many areas of the brain, including the intermediate layers of the superior colliculus (SCi), a midbrain structure that contains a retinotopically coded map for saccades. How TD and BU signals combine or interact within the SCi map to influence saccades remains poorly understood and actively debated. It has been proposed that winner-take-all competition between these signals occurs dynamically within this map to determine the next location for gaze. Here, we examine how TD and BU signals interact spatially within an artificial two-dimensional dynamic winner-take-all neural field model of the SCi to influence saccadic RT (SRT). We measured point images (spatially organized population activity on the SC map) physiologically to inform the TD and BU model parameters. In this model, TD and BU signals interacted nonlinearly within the SCi map to influence SRT via changes to the (1) spatial size or extent of individual signals, (2) peak magnitude of individual signals, (3) total number of competing signals, and (4) the total spatial separation between signals in the visual field. This model reproduced previous behavioral studies of TD and BU influences on SRT and accounted for multiple inconsistencies between them. This is achieved by demonstrating how, under different experimental conditions, the spatial interactions of TD and BU signals can lead to either increases or decreases in SRT. Our results suggest that dynamic winner-take-all modeling with local excitation and distal inhibition in two dimensions accurately reflects both the physiological activity within the SCi map and the behavioral changes in SRT that result from BU and TD manipulations.

Dissociable effects of bottom-up and top-down factors on the processing of unattended fearful faces

While the role of attention in determining the neural fate of unattended emotional items has been investigated in the past, it remains unclear whether bottom-up and top-down factors have differential effects in shaping responses evoked by such stimuli. To study the effects of bottom-up and top-down factors on the processing of neutral and fearful faces, we employed functional magnetic resonance imaging (fMRI) while participants performed attentional tasks that manipulated these factors. To probe the impact of top-down mechanisms on the processing of face distractors, target letters either had to be found among several distinct nontarget letters (attentional load condition) or among identical nontarget letters (baseline condition). To probe the impact of bottom-up factors, we decreased the salience of the targets by reducing their size and contrast relative to baseline (salience condition). Our findings revealed that bottom-up and top-down manipulations produced dissociable effects on amygdala and fusiform gyrus responses to fearful-face distractors when task difficulty was equated. When the attentional load of the main task was high, weaker responses were evoked by fearful-face distractors relative to baseline during the early trials. By contrast, decreasing target salience resulted in increased responses relative to baseline. The present findings suggest that responses evoked by unattended fearful faces are modulated by several factors, including attention and stimulus salience.

Reduced top-down influences in contour detection in schizophrenia

Introduction. Chronic schizophrenia patients have previously demonstrated performance deficits in contour integration tasks. The purpose of this study was to investigate whether schizophrenia patients, spanning a range of illness severity, would demonstrate responsiveness to manipulations that recruit top-down processing strategies involving learning and sequencing effects in a contour integration task. Methods. We administered a contour integration test over four consecutive days and in two different presentation conditions each day. In one condition, the stimuli were administered in order of increasing difficulty, and in the other they were presented in random order. The order in which these two conditions were presented was counterbalanced across days and participants. In addition, a nonschizophrenia psychotic disorders control group was included to determine if past findings of a contour integration deficit in schizophrenia could be replicated in the presence of a symptomatically similar control group. Results. All groups demonstrated similar learning curves across the four days and generally similar overall levels of performance, with the exception of the group of the most chronic schizophrenia patients. In addition, the order in which the stimuli were presented to subjects affected their performance, with higher scores achieved for all groups in the condition where the stimuli were presented in increasing order of difficulty. Interaction effects revealed that the effects of order presentation were greater for nonpatient than for psychotic patients. Conclusions. These data are further evidence that perceptual organization impairments in schizophrenia are illness severity-related, and that schizophrenia patients as a whole are less sensitive to top-down manipulations in this type of task.

Responses of intermittent pond ciliate populations and communities to in situ bottom-up and top-down manipulations

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 42:293-310 (2006) - doi:10.3354/ame042293 Responses of intermittent pond ciliate populations and communities to in situ bottom-up and top-down manipulations Oksana P. Andrushchyshyn, A. Katarina Magnusson, D. Dudley Williams* Surface and Groundwater Ecology Research Group, Department of Life Sciences, University of Toronto at Scarborough, 1265 Military Trail, Ontario M1C 1A4, Canada *Corresponding author. Email: williamsdd@utsc.utoronto.ca ABSTRACT: Pond physicochemical characteristics and bottom-up effects were more important than top-down effects in governing ciliate community structure in 2 adjacent intermittent ponds in Southern Ontario, Canada. The ciliates showed a bimodal seasonal pattern with abundances peaking early and late in the hydroperiods, and the communities showed a strong seasonal succession of species—only 15% of the 162 ciliate species were present throughout the hydroperiods. Less than half of the species occurred in both ponds. Adding riparian leaf litter to large pond enclosures affected several physicochemical variables, increased bacterial abundance, and promoted the appearance of particular species—many of which are known to be associated with nutrient- or organic matter-enriched conditions. This treatment resulted in higher ciliate abundance (mainly small-sized bacterivores) and lower ciliate diversity in mid-hydroperiod in one of the ponds. The removal of plant litter generally produced effects in the physicochemical variables that were opposite to those seen in the leaf litter addition, and resulted in a 15% decrease in the proportion of ciliate bacterivores in one pond. The effects of top-down manipulations (i.e. prevention of aerial colonization of insects) were minor. Many treatment effects were season-, and pond-specific. The measured environmental variables (including pond and treatments) explained half of the variation in ciliate abundance, one-third of the species diversity, and one-fifth of the species composition. Pond characteristics and the leaf litter additions were the most important factors for determining ciliate abundance (together with chlorophyll a), diversity (together with dissolved oxygen), and community composition (together with season). KEY WORDS: Ciliates · Seasonal succession · Temporary ponds · Resource addition · Resource removal · Predator exclusion · Seasonal succession · Top-down · Bottom-up Full text in pdf format PreviousExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 42, No. 3. Online publication date: March 29, 2006 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2006 Inter-Research.

When emotions get the better of us: the effect of contextual top-down processing on matching fingerprints

Twenty-seven participants made a total of 2,484 judgments whether a pair of fingerprints matched or not. A quarter of the trials acted as a control condition. The rest of the trials included top-down influences aimed at biasing the participants to find a match. These manipulations included emotional background stories of crimes and explicitly disturbing photographs from crime scenes, as well as subliminal messages. The data revealed that participants were affected by the top-down manipulations and as a result were more likely to make match judgments. However, the increased likelihood of making match judgments was limited to ambiguous fingerprints. The top-down manipulations were not able to contradict clear non-matching fingerprints. Hence, such contextual information actively biases the ways gaps are filled, but was not sufficient to override clear bottom-up information.

Quality or quantity: the direct and indirect effects of host plants on herbivores and their natural enemies

Resource quality (plant nitrogen) and resource quantity (plant density) have often been argued to be among the most important factors influencing herbivore densities. A difficulty inherent in the studies that manipulate resource quality, by changing nutrient levels, is that resource quantity can be influenced simultaneously, i.e. fertilized plants grow more. In this study we disentangled the potentially confounding effects of plant quality and quantity on herbivore trophic dynamics by separately manipulating nutrients and plant density, while simultaneously reducing pressure from natural enemies (parasitoids) in a fully factorial design. Plant quality of the sea oxeye daisy, Borrichia frutescens, a common coastal species in Florida, was manipulated by adding nitrogen fertilizer to increase and sugar to decrease available nitrogen. Plant density was manipulated by pulling by hand 25 or 50% of Borrichia stems on each plot. Because our main focal herbivore was a gall making fly, Asphondylia borrichiae, which attacks only the apical meristems of plants, manipulating plant nitrogen levels was a convenient and reliable way to change plant quality without impacting quantity because fertilizer and sugar altered plant nitrogen content but not plant density. Our other focal herbivore was a sap-sucker, Pissonotus quadripustulatus, which taps the main veins of leaves. Parasitism of both herbivores was reduced via yellow sticky traps that caught hymenopteran parasitoids. Plant quality significantly affected the per stem density of both herbivores, with fertilization increasing, and sugar decreasing the densities of the two species but stem density manipulations had no significant effects. Parasitoid removal significantly increased the densities of both herbivores. Top-down manipulations resulted in a trophic cascade, as the density of Borrichia stems decreased significantly on parasitoid removal plots. This is because reduced parasitism increases gall density and galls can kill plant stems. In this system, plant quality and natural enemies impact per stem herbivore population densities but plant density does not.