Tooth Bank Research Articles

Influence of root canal taper on the measurement of two different electronic apex locators.

This research aimed to analyze the influence of root canal taper on the accuracy of two Electronic Apex Locators (EALs). Twenty-five disto-vestibular roots from extracted human upper molars belonging to the tooth bank were used in this study. To determine the File Position (FP), access was made using a spherical diamond tip #1014, and the crowns were sectioned using a diamond tip #3080. The initial anatomic file used was a size K #10, which was introduced into the root canal until its tip was visualized (foraminal patency) with the aid of a clinical microscope (16X magnification). Teeth without foraminal patency and calcifications were excluded from the study. Odontometric readings were performed using two different EALs (Root ZX II and Romiapex A-15), considering the electronic reference point 0.0 (apex) for each device. All measurements were taken in triplicate, and the arithmetic mean of the three values was used. Digital calipers were used to record the measurements, which were then entered into an Excel spreadsheet. After visual verification using file K #10, the canals were instrumented with a #25.01 file to standardize the apical region, then successively instrumented with files #25.02, #25.04, #25.06, #25.08, #25.10, and #25.12, with electronic odontometry checked after each instrumentation using #25.02. Measurement 0.0 was adopted, with error margins of ±0.5 and ±1.0. Discrepancies between visual and electronic readings were statistically analyzed using ANOVA and Bonferroni tests, with significance considered when P<0.05. Using the 0.0 mark and a ±1.0 error margin, it was observed that readings from the devices were similar in canals with different tapers (P>0.05), showing a tendency towards underestimation. However, when using the measurement variation margin of ±0.50, a statistically significant difference was found in the Romiapex A-15 group (P=0.0248) when comparing the results of the two EALs. Therefore, it was concluded that the canal taper did not significantly influence the accuracy of the evaluated EALs, using the reference point 0.0. When using the ±0.5 variation margin, the Romiapex A-15 device showed greater accuracy, and finally, at the ±1.0 error margin, both EALs exhibited excellent precision. Key words:Endodontics, Odontometry, Eletronic Apex Locator, Root Canal Preparation.

Dental stem cells and their applications in pediatric dentistry

Regenerative medicine has garnered significant attention due to its transformative impact on disease management and the restoration of optimal bodily functions. Stem cells, a cornerstone of regenerative medicine, are particularly renowned for their remarkable capacity to facilitate tissue regeneration and repair, revolutionizing modern healthcare by offering diverse treatment avenues for multiple conditions. Dental pulp stem cells (DPSCs), a subset of postnatal stem cells, are especially noteworthy for their extensive proliferation and ability to differentiate into various specialized cell types. In the realm of pediatric dentistry, there exists a pressing need for advancements in regenerative medicine. Thorough research in this domain has the potential to propel evidence-based pediatric dentistry, ensuring that children receive tailored, high-quality care. Stem cells hold immense promise in pediatric dentistry by offering less invasive and regenerative solutions for a range of childhood dental issues and congenital anomalies. This research, initiated on 04 October 2023, was instigated following an exhaustive review of existing literature utilizing databases like PubMed, Web of Science, and Cochrane. The literature search encompassed a wide array of medical terminologies. Dental stem cells are categorized into four major types: DPSCs, stem cells from human exfoliated deciduous teeth (SHED), stem cells of the apical papilla (SCAP), and periodontal ligament stem cells (PDLSCs). Pediatric dentistry, encompassing endodontics, orthodontics, periodontics, and the treatment of craniofacial defects, stands to benefit significantly from the potential of dental stem cells. The preservation of dental pulp stem cells extracted from deciduous and permanent teeth through tooth banking offers a source for future regenerative therapies. The preservation process involves multiple comprehensive steps. In summary, dental stem cells present a promising avenue within pediatric dentistry, offering multifaceted applications ranging from pulpal regeneration and dentin repair to orthodontic support and the treatment of craniofacial anomalies.

BANK OF TEETH

Endodontics is the dental specialty responsible for the study of the dental pulp, the entire system of root canals and periapical tissues, as well as the diseases that affect them. for professional training. The purpose of this article was to address didactic-scientific issues regarding the use of human teeth in laboratory teaching and pre-clinical training in the discipline of Endodontics in Dentistry and to report the experience of the Faculdade de Saúde de Petrolina-SOBERANA with the Human Teeth Bank project , implemented as an academic extension project (PEA), with a multidisciplinary character, resulting from the work of the students, under the guidance of the guiding professor. Of the 10 students eligible for this research, 9 used teeth provided by BioBancoVasf, with a 90% success rate. With this, it is concluded that BioBancoVasf is of paramount importance for the university, valuing the tooth as an organ and enabling the execution of more effective practical studies.

Morphological and morphometric characteristics of labial-cervical-vertical groove in upper central incisor

The labial-cervical-vertical groove (LCVG) is a developmental dental anomaly that may be present in the maxillary central incisors (UCI) which can affect periodontal health, facilitate the occurrence cervical decay, and compromise the aesthetics of the gingival contour. A LCVG can present different magnitudes and, when severe, it can impact the prognosis of the affected tooth. Objective: The objective of this study was to investigate the prevalence, extent, and depth of labial-cervical-vertical groove (LCVG) in maxillary central incisors (UCI) from the Human Tooth Bank of Rio de Janeiro State University Dental School, aiming to provide a better understanding of this anomaly and thus to contribute to an accurate differential diagnosis and appropriate dental planning. Material and methods: Ninety-seven dental elements, belonging to the Human Tooth Bank of Rio de Janeiro State University Dental School, were inspected, for presence of LCVG. For analysis of this morphological alteration, the following measurements were registered in millimeters: the total length (measured from the coronal to the apical limits of the defect); its extension in the crown (measured from the defect’s coronal limit to the cementum enamel junction (CEJ)); its extension in the root (measured from the defect’s apical limit to the CEJ) and depth (measurement of the groove’s invagination toward the root canal) of LCVG in each tooth. Results: The prevalence of LCVG in our sample was 7.21%. The average measures observed for the LCVG were: 6.43 (± 1.81) mm of total of length, 2.86 (± 1.68) mm of extension in the root; 3.57 (± 0.98) mm of extension in the crown; and &lt;1 to 1mm of depth. Conclusion: These findings contribute with knowledge on of the detail, of extent and depth of LCVG in UCI, providing the clinician with information that can favor diagnosis and maintenance of teeth with this anomaly.

Investment in Healthcare through Dental Stem Cell Banking in India

Tooth Banking is a simple alternative to recover stem cells with the potential for differentiation into various cells of the body. Young and healthy pulp is a enriched source of stem cells. These cells can be preserved for a longer time to treat various ailments like heart disease, cancer, repairing or even growing damaged organs etc. Likewise, stem cells recovered can be used in regenerative procedures in dentistry. There are many companies offering tooth banking with simple protocols for stem cell banking and storage. This review is based on various dental stem cell banks and details of harvesting with the applications and limitations of procedures. Sources of information are various articles on stem cell banking from reputed journals. Likewise, informative survey of official websites of dental stem cell banks and verbal information extracted from their representatives.

TOOTH BANKING - A REVIEW ARTICLE

Both scientifically and commercially, the area of stem cells has advanced. It is simple and less disruptive to the body to acquire stem cells from teeth. All complex organisms contain the biological cells referred to as stem cells. They can reproduce, differentiate, and rejuvenate them to produce fresh stem cells. For stem cell research, human exfoliated deciduous teeth (SHED) can be acquired quickly and readily with little to no preparation stress. Dental stem cells, the gold standard for neural-crest-derived bone, can be applied as a low-risk autologous therapeutic approach to treat a variety of bodily defects. This study will discuss the banking and therapeutic applications of human exfoliated deciduous tooth stem cells

Obtaining human teeth for dental education: A cross-sectional study to create ethical and transparent processes.

This study explores the practices of obtaining human teeth for teaching and research in Paraguay. The goal of this research is to understand whether the data supports the need to improve ethical training within dental education, including the creation of a human tooth bank (HTB) METHODS: An observational cross-sectional study was conducted. Three semi-structured questionnaires were developed, one for each target population: dental students, dentists in practice, and the general population. The questionnaires were distributed between February and April 2021, using Google Forms RESULTS: The main places where human teeth were obtained by a student are dental clinics, followed by cemeteries. 94% of students and 91% of dentists paid for teeth on at least one occasion. There is a willingness on the part of students and dentists to donate collections of teeth, and among the general population to donate their teeth if they are extracted, for both teaching and research use. Note that, 31% of students and 6% of the population are aware of what an HTB is, at the same time only 16% of dentists know how it functions CONCLUSION: The number of respondents who obtain teeth from cemeteries and who pay for their acquisition is high. Both of these practices raise ethical concerns. The implementation of informed consent for the donation of extracted teeth is practically nil; although there is a positive attitude towards its implementation. Also, there is a lack of knowledge about the role of an HTB; although the population expressed a willingness to donate extracted teeth.

TOOTH BANKING OR DENTAL STEM CELL BANKING &amp; ITS ROLE IN PEDIATRIC DENTISTRY; LITERATUREREVIEW

Stem cell biology has become an integral part of regenerative medicine and dentistry. Since the identication of mesenchymal stem cells, stem cell biology has been a heavily explored area of regenerative medicine and tissue engineered therapies, and has become an integral part of dentistry. Stem cells from deciduous teeth are those obtained with least trauma as deciduous tooth undergoes physiologic exfoliation. Dental stem cell preservation services, “tooth banking,” focus on harvesting a child's baby teeth when they fall out naturally and preserving the stem cells from the pulp for future therapeutic use when the child needs them. The purpose of this review is to focus only on role of stem cells in paediatric dentistry and their banking

Knowledge and Attitude of Undergraduate Students and Interns about Human Tooth Bank: A Cross-sectional Study.

IntroductionHuman tooth banks (HTBs) are philanthropic institutions that assist to alleviate academic pressure by supplying human teeth essential for preclinical laboratory activities and experimental research. The primary focus of this research was to assess the knowledge and attitude of undergraduate students and interns who have taken up dentistry as their field of study in Jazan University, Saudi Arabia, about HTB.Materials and methodsAn online structured self-explanatory questionnaire containing 16 questions was sent via email to the undergraduate students and interns. The Google e-forms questionnaire contained a consent attached for participating in this cross-sectional observational study.ResultsA good response rate of 93.3% was recorded, but the results were surprising as 66.43% of the participants did not have the slightest idea regarding the concept of a tooth bank, while only a meager 17.4% of them supported the idea of getting the teeth preauthorized from HTBs before using it on patients. A majority (80.36%) of students unanimously promoted the idea of having facilities like HTBs, but only 15.36% of students had stored teeth at a teeth bank previously.ConclusionThere is an overwhelming lack of awareness among students regarding HTBs and the legal procedures involved in procuring teeth from them. It is necessary to do all that's required to create awareness about the perks of linking HTB with various dental institutions and the legal process involved to use human teeth.How to cite this articleVishwanathaiah S, Eshaq RH, Maganur PC, et al. Knowledge and Attitude of Undergraduate Students and Interns about Human Tooth Bank: A Cross-sectional Study. Int J Clin Pediatr Dent 2022;15(1):60-64.

Knowledge, Attitude and Practice Regarding Dental Stem Cells and Tooth Banking among Dental Professionals: A Questionnaire-based Cross-sectional Study

Introduction: Stem cell therapy is new revolutionary technique which can repair many defects of human body. There are two sources of stem cells, one is embryonic and postnatal. Postnatal sites are the ones from which procuring stem cells is easiest. Dental stem cells from exfoliated deciduous teeth are also obtained from these sources. Dental health professionals must have understanding about dental stem cells for its application. Hence, there is need to evaluate knowledge, attitude and practice of dental professionals regarding dental stem cells. Aim: To evaluate knowledge, attitude and practice regarding dental stem cells and tooth banking among dental professionals of Gujarat. Materials and Methods: The study was a questionnaire based cross-sectional study conducted in the Department of Paediatric and Preventive Dentistry, KM Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India from December 2020 to March 2021. Total of dental health professionals participated including undergraduate (interns) and postgraduate students, dental practitioners and faculty members of dental colleges. Participants received the questionnaire in the form of a google form through email and whatsApp. The data was analysed using percentages of total and Chi-square test for statistical analysis. Results: Out of 711 participants, 410 were students, 44 faculties, 207 practitioners and 50 faculties and practitioner both. Among the faculty, knowledge on types of dental stem cells was significantly associated with the years of experience (p-value &lt;0.001). Among the students, first year postgraduate students and interns had significantly less knowledge regarding various applications of dental stem cells (p-value &lt;0.001). Among them, 52.9% participants were aware of stem cell banks in India and 55.5% were aware of names of stem cell banks. Participants suggested that seminars, journals, academic curriculum, conferences or Continued Dental Education (CDE), and advertisement and public notice all should be used to increase awareness of dental stem cells among dental professionals. Conclusion: There was adequate awareness among dental health professionals regarding dental stem cells and their applications but were unaware about tooth banking and its procedure. The study also found that there was lack of knowledge regarding steps of preservation of dental stem cells and their ethical concerns. Therefore, the topic of dental stem cells should be included in dental curriculum.

Comparative evaluation of the remineralizing potential of different calcium and fluoride-based delivery systems on artificially demineralized enamel surface; an in vitro study.

Background:Enamel is the hardest and vastly mineralized structure of the human body. Inorganic salts make up the majority of it. Water and ions pass right through the enamel. The enamel undergoes demineralization and remineralization cycle throughout life. Recently, the interest in the development of calcium, phosphate, and fluoride remineralization technology has been increased leading to the development and reintroduction of various remineralizing agents such as fluoride, casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF), nano-hydroxyapatite (nano-HA), xylitol, bioglass, Ozone, etc.Aim:The aim of this study is to evaluate and compare the potential remineralization of CPP-ACPF, calcium sucrose phosphate (CaSP), amine fluoride (AmF), and nano-HA.Settings and Design:The design of this research is experimental. This is an in vitro study. The research was carried out at the Dental Teaching Institute. This study involved human teeth. No demographic data were obtained. One hundred and twenty premolars were selected from the tooth bank of the Department of Oral and Maxillofacial Surgery. The teeth included in the study were noncarious, nonrestored, and nonfractured extracted teeth. Teeth excluded from the study were extracted teeth with any visible or detectable caries, white spot lesions, microcracks, attrition, abrasion, erosion, or abfraction.Materials and Methods:One hundred and twenty enamel samples were taken; they were divided into six groups (n = 20). The demineralization process was carried out on Groups II, III, IV, V, and VI. The remineralization process was carried out on Groups III, IV, V, and VI for 14 days using CASP, AmF, and nano-HA, respectively. No surface treatment was performed in Group I causing it a positive control group, whereas Group II was considered a negative control with only enamel surface demineralization and no remineralization. The microhardness of enamel was measured using Vickers microhardness testing machine after a 14-day remineralization regimen.Statistical Analysis:In the statistical analysis, one-way analysis of variance and post hoc Tukey's tests were performed.Results:The mean microhardness values in declining order: positive control > nano-HA > AmF > CaSP > CPP-ACPF > negative control.Conclusion:All remineralizing agents exhibited enhanced surface remineralization. Nano-HA showed the highest remineralization potential followed by AmF, CaSP, and CPPACPF.

CURRENT TRENDS IN BONE AUGMENTATION DURING DENTAL IMPLANTATION IN PATIENTS WITH PARODONTAL PATHOLOGY AND JAW BONE ATROPHY (LITERATURE REVIEW)

Today, despite the progress made in dental implantology, the problem of rehabilitation of patients with significant bone atrophy in parodontal diseases has not been fully studied.&#x0D; The study aims to review the current views of various authors on the aetiology and treatment of parodontal diseases, which caused multiple tooth loss and bone atrophy in patients with occlusal pathology, based on the literature sources of scientific and medical information.&#x0D; Object and methods of study. Analytical study of scientific and medical literature on the treatment of multiple and partial tooth loss in patients with parodontal diseases in bone atrophy with impaired jaw relationships and orthopaedic rehabilitation using dental implantation and methods of bone augmentation of the mandible and maxilla.&#x0D; The use of implants in orthopaedic treatment expands the possibilities of using fixed prostheses, satisfying patients functionally and aesthetically. In case when the preservation of teeth becomes impossible or impractical to ensure proper treatment of the patient, they can be removed and replaced with dentures on dental implants.&#x0D; According to Misch C. [6], the masticatory load, which is exerted on the bone tissue of the jaws by an implant, increases the density of the bone structure. When teeth are lost due to parodontitis, there is atrophy of the alveolar ridge and a change in the structure of bone tissue, which is characterized by an increase in the porosity of the cancellous bone [4;5;7]. Sufficient bone volume and density in the area of the planned implantation are the main and important parameters for achieving normalized primary implant stabilization [11]. Partial and complete loss of teeth, as well as parodontal diseases, are always accompanied by the bone tissue of alveolar bone tissue of varying severity [1;4;5;13]. Signs of parodontal diseases in dentition defects are always more pronounced than in intact dental arch. The disease progresses rapidly, the dentition is destroyed if appropriate occlusal therapy is not performed [13;14]. Systemic factors and comorbidities in patients also play a significant role in reducing the bone volume of the jaws.&#x0D; The relationship between decreased bone density and age-related hormonal changes in women, as well as at the gender level, has been studied. Thus, women have changes in bone architecture and less dense arrangement of trabeculae in the jawbones than men [15].Bone tissue has a distinguishing feature – loss of multiple teeth or parodontal diseases lead to a redistribution of functional load, which causes its resorption [7;20]. In this regard, tooth extraction inevitably leads to a decrease in bone volume. The success of dental implantation on the maxilla became partially possible due to the introduction of sinus lifting with the use of osteoplastic materials [12;20;21]. According to some scientists[21], the autogenous bone graft is the gold standard in regenerative processes due to its osteoinductive, osteoconductive, and osteointegration properties, which are necessary for the restoration of lost bone tissue.&#x0D; The use of dentin in human teeth as an autograft was first described in a clinical case report in 2003 [23]. In recent experiments, these materials have proven to be a real alternative to the bone graft. Thanks to the work of a group of researchers [23;24], significant efforts were made in the basic and clinical study to find the best bone graft material for osseointegration of implants in the maxillary sinus. The autogenous dentin graft (AutoBT; Korea Tooth Bank, Seoul, Korea) was first developed in 2008, and several clinical studies have praised the use of AutoBT in the sinus compared to other grafts.&#x0D; Given the current development of advanced technologies in dental implantation, dentists use navigation templates in their practice, which are made using CAD CAM technology to improve the quality of care for patients who have indications for dental implantation. New diagnostic approaches allow us to virtually plan, simplify, and speed up surgery, to ensure accuracy. Thanks to this technology, dentists optimize the location of dental implants, taking into account the difficult conditions specific to patients with parodontal pathology.

AVALIAÇÃO DE PROTOCOLOS DE CLAREAMENTO DENTÁRIO MODIFICADOS POR FONTES LUMINOSAS

Introdução: O clareamento dental é o procedimento estético mais realizado nos consultórios odontológicos. O uso de fontes luminosas tem sido recomendado para acelerar a ação do gel de clareamento, entretanto evidências científicas são controversas no que se diz respeito a essas fontes. Objetivo: Avaliar protocolos de clareamento dentário modificados por fontes luminosas, objetivando verificar, qual a influência dessas fontes no procedimento de clareamento dentário. Metodologia: Sendo essa, uma pesquisa que utilizou material biológico, a mesma foi submetida e aprovada pelo Comitê de Ética em Pesquisa da UEPB. Realizou-se a obtenção de dentes por meio de parceria com o Banco de Dentes Humanos do curso de Odontologia da UEPB Campus VIII. Posteriormente realizou-se identificação dos dentes, em seguida registro inicial da cor do dente, o preparo dos blocos dentários e seleção dos grupos. Os dentes foram submetidos a dois protocolos de clareamentos distintos. Por fim, realizada a análise estatística. Resultados: A modificação do protocolo, seja quanto o tempo de aplicação do agente clareador, seja quanto à fonte de luz utilizada, alcança resultados satisfatórios. Não houve diferença estatisticamente significativa entre os grupos. Conclusões: Tendo em vista os resultados e a discussão encontrados na pesquisa, podemos verificar que as evidências científicas nos levam a observar que não há bases suficientes que comprovem a eficácia e benefícios a curto prazo da modificação dos protocolos de clareamento dental pelas fontes luminosas.

Knowledge of Teacher and Dentistry Students of a Brazilian University about a Human Teeth Bank

Human Tooth Banks (HTB) tries to overbear academic activities by providing human teeth, which are fundamental for preclinical, clinical and experimental applications. This study, which took place at the Federal University of Ceara-UFC, considered the cognition of students and professors of Dentistry on HTB. Aim: This study aimed at the investigation of the reality and importance of this tool for the undergraduate course, research as well as for the legal acquisition of this dental organ. Methods: This is a quantitative study, that a semi-structured questionnaire was used. The Statistical Package for the Social Sciences program analyzed all the data, considering the reliability of 95% and using Fisher’s Exact test or the Person Chi-square test. Results: Although only 6.4% of the participants were familiarized with HTB, 99.1% considered their existence important. As for the use of human teeth, 93.5% reported having used HTB during the course, but only 41.1% requested authorization to handle. Conclusion: The knowledge of students and professors of Dentistry about HTB and its legality in obtaining this body is insufficient, although the use of human teeth and its high importance is attributed to the existence of HTB in dental schools.

Influence of a new method of sterilization on the morphology and physical properties of extracted human teeth

Abstract Introduction Sterilization methods adopted in Human Teeth Bank can promote structural changes in enamel and dentin. Thus, the ideal method should combine the antimicrobial efficacy and preservation of the substrate biomechanical properties. Aim Evaluated the morphology and physical properties of enamel and dentin of extracted human teeth, after being submitted to different sterilization methods. Method Sixteen extracted third molars were sectioned in the following tooth regions: mesio-distal, vestibular-lingual and at the cement enamel junction. Forty specimens were selected according to values of microhardness and roughness initials, and distributed in four experimental groups (n = 10/group): Group 1 = Autoclave 121 °C (30 minutes); Group 2 = 2.5% Sodium hypochlorite (07 days); Group 3 = 5.25% Sodium hypochlorite (07 days); Group 4= 30% Acetic Acid (7 days). After sterilization, the microhardness, roughness and morphology of enamel and dentin were evaluated. Data were analyzed by paired t-test, Wilcoxon, Kruskal-Wallis and Analysis of Variance. A significance level of 5% was adopted. Result All sterilization methods altered the microhardness and roughness of the dentine (p &lt;0.05). Regarding to enamel, the microhardness was affected only by Autoclave and 2.5% Sodium hypochlorite treatment (p &lt;0.05). A large aperture of the enamel pores and dentinal tubules was verified in the micrographs. Conclusion The sterilization methods adopted altered the morphology and/or physical properties of enamel and dentine.

Histological Evaluation of the Healing Process of Various Bone Graft Materials after Engraftment into the Human Body.

The purpose of this study was to measure the level of new bone formation induced by various bone graft materials to provide clinicians with more choices. The samples were divided into three groups: group 1 (n = 9: allograft + xenograft, DBX®, San Francisco, CA, USA + Bio-Oss®, Princeton, NJ, USA), group 2 (n = 10: xenograft, Bio-Oss®), and group 3 (n = 8: autogenous tooth bone graft, AutoBT®, Korea Tooth Bank, Seoul, Korea). The average duration of evaluation was 9.56, 2.50, and 3.38 months, respectively. A tissue sample was taken from 27 patients during the second implant surgery. New bone formation was measured via histomorphometry, using a charge-coupled device camera, adaptor, and image analysis software. Total bone area, total area, and ((total bone area/total area) × 100) was measured to determine the extent of new bone formation. The mean value of the total bone area was 152,232.63 μm2; the mean value of the total area was 1,153,696.46 μm2; and the mean total bone area/total area ratio was 13.50%. In each comparison, there was no significant difference among the groups; no inflammation or complications were found in any of the groups. AutoBT®, an autogenous tooth bone graft, resulted in a level of bone formation similar to that using allografts and xenografts.

Conhecimento dos cirurgiões-dentistas sobre Banco de Dentes Humanos

Banco de dentes humanos (BDH) é uma entidade sem fins lucrativos que tem a finalidade de recepcionar, armazenar, catalogar e manter em boa conservação dentes humanos para pesquisa e treinamento laboratorial pré-clínico de estudantes. No entanto, muitos cirurgiões-dentistas desconhecem a importância do BDH. O objetivo deste estudo foi avaliar o conhecimento dos cirurgiões-dentistas das Unidades Básicas de Saúde (UBS) do município de Teresina/PI sobre condutas diversas relacionadas aos BDHs. O presente estudo é do tipo observacional com amostragem probabilística estratificada com alocação proporcional, que utilizou entrevistas. Mais da metade dos cirurgiões-dentistas afirmaram ter conhecimento da existência de BDHs no Brasil, porém 81% desconheciam a existência do BDH da Universidade Federal do Piauí (UFPI). Todos os entrevistados foram a favor da doação e consideram o dente como um órgão, entretanto 95,2% nunca fez doação de dentes para um BDH. O lixo foi o destino mais comum após exodontias. Diante disto, foi possível concluir que não há doação de dentes humanos pelos cirurgiões-dentistas entrevistados, possivelmente, devido ao desconhecimento da existência do BDH da UFPI e suas peculiaridades.

The evaluation of the transport medium for extracted premolars prior to cryopreservation: a systematic literature review.

Prior to cryopreservation, a tooth is transported from a contaminated oral environment to the tooth bank. Our objective was to identify all studies reporting or investigating a transport protocol prior to the cryopreservation of teeth, in terms of decontamination of the subjects. The systematic literature search (1970-2017) was based on MEDLINE via PubMed, Web of Science and the Cochrane Library. The reference lists of the included studies and the Science Citation Index were used for hand searching (snowballing). Only studies reporting the transport conditions of the transplant were included. Language restrictions for English, Dutch or French were applied. The search led to 14 eligible studies. Almost all studies were laboratory studies, so the methodological quality of evidence was low. The majority of the included studies was performed by only five different research groups and the number of subjects varied between 1 and 120 teeth. In general, the teeth were stored in a tissue culture medium supplemented with fetal calf serum and/or different combinations of antibiotics and/or antimycotics. The teeth were transported cooled (4°C) or at room temperature, for a period of time not exceeding 24h. Only three studies reported the irrigation of the teeth with phosphate buffered saline prior to the transport. The optimisation of the decontamination during transport was investigated in three studies (from 1971, 1980 and 1982). It was concluded that the literature on this topic is scarce, and the decontamination protocol for teeth, prior to cryopreservation has not been validated recently.

Avaliação de métodos de manutenção da esterilidade do órgão dental humano extraído para armazenamento em banco de dentes

A importância de um banco de dentes humanos em uma instituição de se justifica e se mostra vantajosa, uma vez que permite a máxima aproximação da realidade ao se trabalhar com o órgão extraído. Este estudo teve o propósito de avaliar métodos de manutenção da esterilidade do órgão dental humano extraído armazenado. Foram utilizados 72 dentes incisivos humanos extraídos, obtidos em clínicas de graduação e de pós-graduação da Universidade Positivo, Curitiba, PR, Brasil. Os elementos dentários foram cedidos pelos pacientes, por meio de termo de doação devidamente assinado. Após os procedimentos de limpeza e de desinfecção, 36 dentes foram esterilizados em autoclave e 36 foram somente limpos. Os dentes foram, então, armazenados em recipientes contendo o método (autoclavagem ou limpeza) ou a solução de escolha, por um período de 15 e 120 dias. Testes microbiológicos foram realizados a fim de determinar qual método ou solução de armazenamento promove a manutenção da esterilidade no tempo determinado. Melhores desempenhos foram observados quando as amostras foram armazenadas em Incidin Extra N®, formol e álcool, mesmo para os dentes não autoclavados. As substâncias em análise nos períodos propostos se mostraram capazes de impedir o crescimento microbiano. Este experimento poderá auxiliar o desenvolvimento de um protocolo de processamento e de administração do órgão dental humano extraído em um banco de dentes.

Effect on bone formation of the autogenous tooth graft in the treatment of peri-implant vertical bone defects in the minipigs.

BackgroundThe aim of this study was to evaluate the effect of autogenous tooth bone as a graft material for regeneration of bone in vertical bony defects of the minipigs.Material and MethodsSix minipigs were used in this study. Four molars were extracted in the right mandibular dentition and sent to the Korea Tooth Bank for fabrication of autogenous tooth bone. Ten days later, each extraction site was implanted with MS Implant Narrow Ridge 3.0x10mm fixture (Osstem, Seoul, Korea) after standardized 2mm-sized artificial vertical bony defect formation. Pineappleshaped Root-On type autogenous tooth bones were applied to the vertical defects around the neck area of the posterior three fixtures and the fore-most one was not applied with autogenous bone as a control group. Each minipig was sacrificed at 4, 8, 12 weeks after fixture installation and examined radiologically and histologically. Histological evaluation was done under light microscope with Villanueva osteochrome bone staining with semi-quantitative histomorphometric study. Percentage of new bone over total area (NBF) and bone to implant contact (BIC) ratio were evaluated using digital software for area calculation.ResultNBF were 48.15 ± 18.02%, 45.50 ± 28.37%, and 77.13 ± 15.30% in 4, 8, and 12 weeks, respectively for experimental groups. The control group showed 37.00 ± 11.53%, 32.25 ± 26.99%, and 1.33 ± 2.31% in 4,8,12 weeks, respectively. BIC ratio were 53.08 ± 19.82%, 45.00 ± 28.37%, and 75.13 ± 16.55% in 4,8,12 weeks, respectively. Those for the control groups were 38.33 ± 6.43%, 33.50 ± 29.51 %, and 1.33 ± 2.31% in 4, 8, 12 weeks, respectively.ConclusionAutogenous tooth bone showed higher score than control group in NBF and BIC in all the data encompassing 4,8,12 weeks specimens, but statistically significant only 12 weeks data in both NBF and BIC.