Tonifying Kidney Research Articles

Traditional Chinese medicine tonifying kidney therapy (Bu shen) for stable chronic obstructive pulmonary disease: Protocol for a systematic review and meta-analysis.

Background:Traditional Chinese medicine (TCM) is commonly used to combine with pharmacotherapy for stable chronic obstructive pulmonary disease (COPD) in China and other Asian countries such as South Korea and Japan. The objective of this systematic review is to evaluate the efficacy and safety of tonifying kidney therapy (Bushen, TK) for stable COPD.Methods:Randomized controlled trials (RCTs) of TK for stable COPD were searched from 4 databases including Pubmed, the Cochrane library, CBM (China Biology Medicine disc, CBMdisc), CNKI (China National Knowledge Infrastructure) from inception to December 2017. Two reviewers independently screened the literature, extracted the data and assessed the risk of bias in included studies. RevMan 5.3 software was used for meta-analysis. The primary outcomes analyzed in this meta-analysis were effectiveness, TCM Syndrome Score, dyspnea (modified Medical Research Council questionnaire [mMRC]), COPD health status (COPD Assessment Test [CAT]), exercise capacity (6-min walk distance in meters [6mWD]), and respiratory-specific quality of life (St George's Respiratory Questionnaire [SGRQ]). Second outcomes analyzed for this meta-analysis were lung function (forced expiratory volume in 1 second [FEV1], FEV1%, forced vital capacity [FVC], FEV1/FVC), the frequency of acute exacerbation, T-lymphocyte subsets (CD4, CD8, CD4/CD8), and immunoglobulin (IgA, IgG, and IgM). The summary results will be pooled using the random-effects model or fixed-effects model according to the heterogeneity of the included studies.Result:This systematic review will provide an evidence of TK for stable COPD, and will submit to a peer-reviewed journal for publication.Conclusion:The conclusion of this systematic review will provide evidence to judge whether TK is an effective intervention for stable COPD patients.PROSPERO registration number:PROSPERO CRD 42018090328.

Protective Effects of Total Glycoside From Rehmannia glutinosa Leaves on Diabetic Nephropathy Rats via Regulating the Metabolic Profiling and Modulating the TGF-β1 and Wnt/β-Catenin Signaling Pathway.

Rehmannia glutinosa Libosch (RG), is officially listed in the Chinese Pharmacopoeia and is widely used in China. The leaves of RG (LR) is an important vegetative organ of the plant. At present, the total glycosides of RG (TLR) were extracted from RG, and developed a national second class of new drugs to the Dihuangye total glycoside capsule (DTG). Additionally, DTG has the effect of nourishing yin and tonifying kidney, promoting blood circulation and blood cooling, and applicable to chronic glomerulonephritis mild to Qi and Yin Deficiency. Moreover, diabetic nephropathy (DN) rats model was induced by intraperitoneal injection of a small dose of streptozotocin (45 mg/kg) and high-fat diet and plus 5% glucose drinking water. Over 15 days, after oral administration TLR and DTG in DN rats, samples from serum, urine and kidney were collected for biochemical indicators measurements, pathological analysis, western blotting and metabolomics. Therefore, the analytical results of biochemical indicators, histopathological observations and western blotting showed that TLR and DTG exhibited a significant effect in renal protection. And 27 endogenous metabolites (12 in serum and 15 in urine) could be tentatively identified in the process of DN in rats using metabolomics method. Those endogenous metabolites were chiefly involved in sphingolipid metabolism; pentose, glucuronate interconversion; terpenoid backbone biosynthesis; purine metabolism and retinol metabolism. After drug intervention, these endogenous metabolites turned back to normal level some extent (P < 0.05). Furthermore, TLR and DTG prevent high glucose-induced glomerular mesangial cells (GMCs) by inhibiting TGF-β1 and Wnt/β-catenin signaling pathway, providing a powerful supports to develop a new therapeutic agent for DN. This study paved the way for further exploration of the pathogenesis of DN, early diagnosis and the evaluation of curative effect.

Experimental Study on the Effect of Black Glutinous Rice Health Wine on Tonifying Kidney in Mice

Experimental Study on the Effect of Black Glutinous Rice Health Wine on Tonifying Kidney in Mice

Research of the medication rules of ankylosing spondylitis based on the famous doctors' medical records

Objective Applying the traditional Chinese medical knowledge service and sharing system, to explore the medication rules of ankylosing spondylitis in the medical records. Methods The ankylosing spondylitis medical records in the traditional chinese medical knowledge service and sharing system has been searched and 149 prescriptions were identified. The medication frequency, association rules, herbal combination and the cluster analyses were explored. Results Among the 149 prescriptions with 210 drugs, the use of high frequency drugs were Danggui, Duzhong, Weilingxian, and other medication whose frequency was more than 20 times were good at tonifying liver and kidney, activating blood circulation, clearing heat and promoting diuresis, dispelling cold and removing dampness. The high frequency of drug combinations included Sangjisheng and Gouji, Duzhong and Gouji, Baishao and Gouji, Guizhi and Baishao, Gancao and Danggui. Conclusions The treatment of ankylosing spondylitis with Chinese medicine is mainly based on tonifying liver and kidney, supplemented with drugs for warming Kidney Yang, dispelling cold and dehumidification, promoting blood circulation and removing blood stasis, clearing heat and promoting diuresis, dispelling wind and cold. Key words: Formula analysis, computer assisted; Famous doctor's experience; Spondylitis, ankylosing; Data mining; Medication regularity; The traditional Chinese medical knowledge service and sharing system

Phenylethanoid Glycosides of Cistanche on menopausal syndrome model in mice

Cistanche is the traditional and precious Chinese herbal, with two thousand years of use history in China. It has the effect on tonifying kidney, strong supplement to the liver and kidney, and replenishing essence and blood, known as the “desert ginseng”. Here, we explored the mechanism of Phenylethanoid Glycosides of Cistanche (PGC) to the model mice of menopausal syndrome, as well as the therapeutic effect and characteristics of PGC to the menopausal syndrome. In this study, KM mice were reproduced by the complete resection of the ovaries on both sides of the back to establish the model mice of menopausal syndrome (MPS), and received distilled water or drugs, respectively. Model mice received distilled water. Mice received 200mg/(kgday) high doses of Phenylethanoid Glycosides of Cistanche (HPGC), and 100mg/(kgday) medium doses of Phenylethanoid Glycosides of Cistanche (MPGC), and 50mg/(kgday) low doses of Phenylethanoid Glycosides of Cistanche (LPGC). After 21days, it could determine the number of independent activities and the number of standing, the latent period of first entering the dark room, and the electric number. It also calculated the viscera index of uterus, thymus, spleen, measured the levels of estradiol (E2), testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) in the serum. Furthermore, it observed the pathological changes of uterus, thymus, spleen and pituitary of mice. The results showed that behavioral indicators: Compared with the model group (MG), HPGC, MPGC, LPGC could increase the independent activities (P<0.01); HPGC, MPGC could increase the number of standing, the latent period of first entering the dark room, and reduce the electric number (P<0.01); LPGC could increase the number of standing (P<0.05); Viscera index: Compared with MG, HPGC, MPGC could increase the viscera index of uterus, thymus, spleen (P<0.01); LPGC could increase the viscera index of uterus (P<0.05); Serum index: Compared with MG, all groups could decrease the levels of LH in the serum (P<0.01); HPGC, MPGC could improve the levels of E2, T and decrease the levels of LH, FSH in the serum (P<0.01); LPGC could improve the levels of E2 and decrease the levels of FSH in the serum (P<0.05). Meanwhile, it had the trend to improve the levels of T in the serum. Pathological changes: Compared with MG, HPGC could significant improve the pathological changes of uterus, thymus, spleen and pituitary of mice; other groups also has a certain effect. The results indicated that PGC could improve the sex hormone disorder of MPS, and restore the function of uterus, thymus and spleen, with better therapeutic effect on MPS.

曲秀芬教授运用中药调周治疗月经过少经验

月经过少发病率逐年上升，已成为影响女性身心健康的关键问题。月经过少者往往存在生殖内分泌功能紊乱或子宫内膜受损，因此孕卵很难着床，如果不予足够重视延误治疗时机，易发展为闭经，导致不孕。曲秀芬教授结合自己多年临床经验和月经过少这一疾病的特点，认为该病以肾虚为本，主张顺应气血阴阳的消长变化和胞宫的生理功能，以补肾为核心，运用中药调周治疗，临床疗效显著。 Hypomenorrhea has become a key problem which affects women’s physical and mental health; the incidence of hypomenorrhea rises year by year. Hypomenorrhea patients tend to have reproductive endocrine dysfunction or endometrial damage. Therefore, it is difficult for pregnant eggs to be implanted. If we do not pay enough attention to the disease and delay treatment time, it may lead to amenorrhea and infertility. Professor Qu Xiufen combines her years of clinical experience and the characteristics of hypomenorrhea, and thinks that the disease always base on deficiency of the kidney. She maintains that we should conform to the change of Qi, Blood, Yin and Yang and the physiological function of the uterus. She takes tonifying kidney as the core and treats hypomenorrhea based on the traditional Chinese medicine cyclic therapy. The clinical effect is remarkable.

Professor SHI Yin's experience of staging, classification and sorting method for polycystic ovary syndrome

The clinical experience of professor SHI Yin for polycystic ovary syndrome (PCOS) was summarized. According to the main pathogenesis of PCOS, the tonifying kidney should be taken as essence with synchronous treatment on liver, spleen and heart, presenting staging, classification and sorting method for PCOS. In the staging method, the regulation on follicle development should be taken as treatment core to comply with the rules of yin and yang. A four-stage method was proposed, where "regulating method" was suitable in menstrual period, "tonifying method" in follicular phase;"dredging method" in ovulatory period and "adjustment and tonifying " in luteal phase. In the classification and sorting method, attention was paid on individualized treatment, and treatment was based on fat type, thin type and non-fat type as well as childbearing. Besides, psychological counseling and life adjustment for patient was essential, and the unity of body and mind could enhance curative effect.

A pair of taxifolin-3-O-arabinofuranoside isomers from Juglans regia L.

Diaphragma juglandis Fructus has been known for its effect on tonifying kidney in traditional Uighur medicine. During our ongoing studies on the discovery of biologically active compounds from folk medicine, a pair of flavanonol glycoside isomers named (2S,3S)-taxifolin-3-O-α-d-arabinofuranoside (1) and (2S,3S)-taxifolin-3-O-α-l-arabinofuranoside (2) were isolated, among them, 1 was a new compound. It is the first report of taxifolin-3-O-arabinofuranosides in both α-d and α-l configuration forms in genus Juglans. Their structures were determined on the basis of spectroscopic analysis and chemical evidence, in combination with circular dichroism.

The study of the eosinophil CD34+ progenitor cells differentiation mechanism of model rats with occupational asthma and the intervention of warm and tonifying kidney yang decoction

Objective: To investigate the mechanism of CD34+ progenitor cell differentiation in rat by observing the change relations between the eosinophils (EOS) and the content of Eotaxin and IL-5 in blood and the CD34+/CCR3+, CD34+/IL-5Rα+ in bone marrow after occupational asthma (OA) model rats are simulated, and to observe the effect of WTKYD Trraitional Chinese Medicine intervention. Methods: A total of 40 healthy male SD model rats (200~250 g weight) were randomly divided into model contrast Group, prednisone acetate intervention Group, WTKYD+1/2 prednisone acetate intervention Group and WTKYD intervention Group, 10 in each group, and set a Group for blank contrast. Give them saline (20 ml/kg) , prednisone acetate (8.22 mg/kg) , WTKYD (20g/kg) +1/2 prednisone acetate (4.11 mg/kg) and WTKYD (20 g/kg) intervention respectively. By means of cell count, immunohistochemical, ELISA, flow cytometry technique, situ hybridization and so on, to observe EOS and the expression of Eotaxin in lung tissue, the EOS in peripheral blood, the content of Eotaxin and IL-5 in blood as well as the expression of CD34+/CCR3+ and CD34+/IL-5Ra+ in bone marrow respectively. Results: The number of EOS, the content of Eotaxin and IL-5, the expression of CD34+/CCR3+ and CD34+/IL-5Ra+ in Model Contrast Group were higher in Blank Contrast Group, the difference was statistically significant (P<0.01) , while they were lower in medical intervention Groups when comparing to Model Contrast Group, the difference was statistically significant (P<0.01 or P<0.05) , and the above items in WTKYD+1/2 Prednisone Acetate Intervention Group were even lower than in Prednisone Acetate Intervention Group and WTKYD Intervention Group, the difference was statistically significant (P<0.05). EOS in lung tissue is highly positive related to the content of Eotaxin and IL-5 in peripheral blood as well as the expression of CD34+/CCR3 and CD34+/IL-5Rα in bone marrow (0.9666、0.9829、0.9142, 0.8874). Conclusion: The increase of internal EOS in lung tissue is related to the up-regulated expression of CD34+/CCR3+ and CD34+/IL-5Ra+ in bone marrow after antigens in Occupational Asthma model rats are stimulated. Through down-regulating it's expression to restrain the differentiation of CD34+ progenitor cells towards EOS, meanwhile, the collaboration of WTKYD and prednisone acetate possess a certain synergistic action.

A water extract of Malva verticillata seeds suppresses osteoclastogenesis and bone resorption stimulated by RANK ligand

BackgroundMalva verticillata seeds are used as a therapeutic medicine to treat kidney dysfunction in traditional Chinese medicine (TCM). TCM has suggested that herbal medicine tonifying kidney function may have beneficial effect on bone metabolism.MethodsOsteoclastogenesis was examined in bone marrow macrophages by measuring tartrate-resistant acid phosphatase (TRAP) activity and counting the number of TRAP-stained multinuclear cells. The activation of receptor activator of nuclear factor-kB (RANK) ligand signaling, and the expression of c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) were investigated by western blot analysis. Transcription factor and bone resorption marker mRNA levels were evaluated using real-time quantitative polymerase chain reaction. The bone resorption activity of mature osteoclast was examined in osteoclasts cultured on a hydroxyapatite-coated culture plate.ResultsA water extract of M. verticillata seeds (WEMV) inhibited osteoclastogenesis stimulated by RANKL. WEMV also strongly inhibited expression of c-Fos and NFATc1 as well as phosphorylation of c-Jun N-terminal kinase, p38, I-kBα, and phospholipase γ2. Furthermore, WEMV significantly attenuated osteoclast resorption activity and downregulated mRNA expression of resorption markers.ConclusionThese results demonstrate that WEMV inhibits osteoclastogenesis and bone resorption by suppressing the RANKL signaling pathway and suggest that M. verticillata seeds may be used as a therapeutic candidate in complementary alternative medicine to treat pathological bone diseases.

Effect of Chinese Materia Medica with Tonifying Kidney Function on Transplantation of Multipotency Mesenchymal Stem Cells from Human Umbilical Cord in Mice Model of Acute Kidney Injury

ObjectiveMesenchymal stem cells (MSCs) represent a promising population for supporting new clinical concepts in cellular therapy, and they can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. MethodsThis method opened up a new era of stem cell research, because the transplantation rejection of iPSCs is the bottleneck of its clinical application, so seeking alternative compounds and animal origin diagnostic reagents to achieve full chemical iPSCs is to be done to solve this problem. ResultsThe application of these iPSCs has largely been associated with well known undesirable effects such as the development of cancers in certain experimental models. This has called for the search and use of reprogramming factors that are safe. Chinese materia medica (CMM) with tonifying kidney function (TKF) offers an alternative source. On the other hand, human umbilical cord mesenchymal stem cells (hUMSCs) are known to be a “young” source of MSCs, hUMSCs transplantation is an attractive approach for acute kidney injury repair. Therefore, In this study, we investigated whether the treatment of CMM with TKF on hUMSCs could enhance the repair in mice model of acute kidney injury after transplantation. ConclusionOur results showed that the treatment of hUMSCs with kidney tonifying CMM increased their multipotency, improved the renal function of mice and enhanced subsequent homing to the injured kidney in an acute kidney injury mice model.

Postoperative complications of microsurgery combined with dispersing stagnated liver, strengthening spleen and tonifying kidney in varicocele associated infertility

Objective Compare the postoperative complications of some kinds of surgical approaches and dispersing stagnated liver, strengthening spleen and tonifying kidney to varicocele associated infertility. Methods From 204 varicocele associated infertility hospitalized patients, which were randomly devided into 4 groups.51 cases of high ligation of spermatic vein with laparoscope(group A);50 cases of ligation of spermatic vein with microscope(group B); 52 cases of microscope with the traditional Chinese medicine(group C);51 cases of microscope with the traditional Chinese medicine(group D), compared postoperative complications(unwell in scrotum, scrotal edemas, hydrocele)after the operations of one week,one month, three months, six months and twelve months in four groups and recurrence of half a year. Results The rate of symptom improvement in D group was significantly better than the other three groups(P<0.05).Over time, there was no statistical difference between four groups. The recurrence rate of D group(0.00%)was obviously lower than A group(11.76%), the B group(2.00%)and the C group(3.85%)(P<0.05). Conclusion Microscope with the traditional Chinese medicine is a good choice in preventing postoperative the rate of symptom and recurrence. Key words: Varicocele; infertility; dispersing stagnated liver qi strengthening spleen tonifying kidney; microsurgery; laparoscopy; complications

Analysis on medication regularity of modern traditional Chinese medicines in treating melancholia based on data mining technology

To analyze the prescription and medication regularities of traditional Chinese medicines in the treatment of melancholia in the Chinese journal full text database (CNKI), Wanfang Data knowledge service platform, VIP, Chinese biomedical literature database (CBM) in based on the traditional Chinese medicine inheritance support platform software, in order to provide reference for further mining traditional Chinese medicines for the treatment of melancholia and new drug development. The traditional Chinese medicine inheritance support platform software V2.0 was used to establish the prescription database of traditional Chinese medicines for treating melancholia. The software integrated data mining method was adopted to analyze four Qis, five flavors, meridian distribution, frequency statistics, syndrome distribution, composition regularity and new prescriptions. Totally 358 prescriptions for treating melancholia were analyzed to determine the frequency of prescription drugs, commonly used drug pairs and combinations and develop 22 new prescriptions. According to this study, prescriptions for treating depression collected in modern literature databases mainly have the effects in soothing liver and resolving melancholia, strengthening spleen and eliminating phlegm, activating and replenishing blood, regulating liver qi, tonifying spleen qi, clearing heat and purging heat, soothing the mind, nourishing yin and tonifying kidney, with neutral drug property and sweet or bitter flavor, and follow the melancholia treatment principle of "regulating qi and opening the mind, regulating qi and empathy".

Professor JIA Chun-sheng's case report and his academic thought of invigorating spleen to nourish original qi

Under the influence of academic theories of numerous physicians of TCM such as Li Gao and Li Zhong-zi in antient China, Professor Jia Chun-sheng proposed the academic thought of tonifying spleen and kidney in combination with acupuncture based on his clinical experience for decades. In clinical practice of acupuncture, Zhōngwăn (▪ CV 12), bilateral Tiānshū (▪ ST 25), Qìhăi (▪ CV 6), Guānyuán (▪ CV4) are often selected to invigorate spleen so as to nourish original qi and to treat both spleen and kidney. The above-mentioned five acupoints form a pattern similar to a star according to their location distribution, so it is called the starlike selection method of acupoints, which attaches importance to spleen and kidney (original qi). Many diseases, especially internal injuries, are often treated with the starlike acupuncture manipulation combined with acupuncture at other acupoints, which achieves good clinical efficacy.

Possible mechanism underlying the effect of Heshouwuyin, a tonifying kidney herb, on sperm quality in aging rats.

BackgroundHerb mixtures are used as alternatives to hormone therapy in China for the treatment of partial androgen deficiency in aging men. However, the compositions of these herb mixtures are complex and their mechanisms are often unknown. This study investigates the effect of Heshouwuyin, a Chinese herbal compound for invigorating the kidney, on the control of testosterone secretion and sperm function.MethodsAged Wistar rats were administered with Heshouwuyin. A Shouwu pill group and young group were used as controls.ResultsMorphology, chemiluminescence, fluorescence immunohistochemistry, and western blot showed that the epididymal sperm of naturally aged rats had intact plasma membranes. They also had abnormal mitochondrial function and DNA integrity, a significant decline in serum testosterone levels, and significant pathological changes in the structure of testicular tissues. Heshouwuyin significantly improved sperm function and serum testosterone levels, and improved testicular morphology. Moreover, the curative efficacy of Heshouwuyin after 60 days was better than that of Heshouwuyin after 30 days and the Shouwu pill group.ConclusionHeshouwuyin exerts an important role in controlling testosterone secretion and sperm function.

Research on the effects of warming spleen and tonifying kidney combined with HAART on immune function of HIV/AIDS patients

Objective To investigate the effects of warming spleen and tonifying kidney combined with highly active antiretroviral therapy (HAART) on immune function of human immunodeficiency virus (HIV)/ acquired immunodeficiency syndrome (AIDS) patients.Methods 30 patients with HIV/AIDS were divided into integrated traditional Chinese and western medicine treatment group (the treatment group) with 14 cases,western medicine control group (the control group) with 16 cases.The control group was given HAART,while the treatment group was given warming spleen and tonifying kidney combined with HAART.The treatment course for both groups was six months.Karovsky Performance Status,clinical symptoms,CD4+T cell count,CD4+/CD8+,blood routine,liver and kidney function of two groups were observed before and after treatment,and we compared the clinical efficacy of two groups.Results ① Clinical benefit rate of treatment group and control group were 100％ and 87.5％ respectively,the difference between 2 groups was statistically significant (P ＜ 0.05); ② Karovsky performance status and weight were improved in the treatment group (P ＜ 0.05) and the TCM symptoms asthenia,the total clinical syndrome scores were better than those of control group after the treatment (all P ＜ 0.05).③ CD4+ cell and white blood cell count of the treatment group were (264.97 ± 52.94)/mm3 and (6.94 ± 0.97)109/ L,which were better than (238.73 ± 50.13)/mm3 and (5.57 ± 1.29) 109/L in the control group (P ＜ 0.05); ④ There was no obvious adverse reaction during treatment in two groups.Conclusion Warming spleen and tonifying kidney combined with HAART therapy can significantly improve the clinical symptoms of HIV/AIDS patients,and can enhance immune function and improve their quality of life,which may play a certain role in realization functional cure of HIV/AIDS. Key words: Warming spleen and tonifying kidney; Traditional Chinese medicine; Human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS); Immunity

Doctor Ma Kun’s experience of applying tonifying kidney and promoting blood circulation treatment of anovulatory infertility

With the ascending attack rate of anovulatory infertility year by year, people also began to pay attention to its treat methods. According to Doctor Ma Kun,who are engaged in clinical work about the treatment for anovulatory infertility, kidney deficiency is the basic pathogenesis and blood stasis is an important factor that has been through. Flexible use of tonifying the kidney and promoting blood circulation treatment of anovulatory infertility in clinic, has achieved remarkable curative effect. Director Ma adjusts menstruation by the different periods, and regulates both patients' negative emotions and sleep quality. Through years of clinical experience accumulation, Director Ma gradually formes special treatment of anovulatory infertility by flexibly using of tonifying the kidney and promoting blood circulation individually.

Impact and long-term effect of three prescriptions regulating and tonifying lung and kidney on JAK/STAT signaling in rats

To evaluate the impact and long-term effect of three prescriptions regulating and tonifying lung and kidney (prescription tonifying lung and spleen, prescription tonifying lung and kidney, and prescription tonifying Qi and kidney) on JAK/STAT signaling of COPD rats. Rats were randomly divided into the control group, the model group, the Bufeijianpi group, the Bufeiyishen group, the Yiqizishen group and the aminophyline group. The COPD rat model was established by smoke inhalations and bacterial infections. In the 9th week, the control group and the model group were administered with normal saline, while the remaining groups are orally given corresponding medicines. In the 20th and 32nd week, the rats were sacrificed in batches to observe the pathology in their lung tissues, protein expressions of JAK2, STAT1, STAT3, STAT5, and expressions of JAK2 and SOCS3 mRNA. In the 20th and 32nd week, protein expressions of JAK2 mRNA and phosphorylation-JAK2, STAT1, STAT3 and STAT5 in the model group were higher than the control group (P < 0.01), whereas the three traditional Chinese medicine (TCM) (Bufeijianpi, Bufeiyishen and Yiqizishen) groups and the aminophyline group were significantly lower (P < 0.05, P < 0.01). The expression of SOCS3 mRNA in the model group was higher than the control group (P < 0.01), whereas the level was notably higher in the three TCM groups and the aminophylline group (P < 0.01). The three TCM groups were remarkably higher than the aminophylline group (P < 0.05, P < 0.01). Compared with the figures in the 20th week, JAK2 mRNA and phosphorylation-JAK2, STAT3 and STAT5 were significantly lower in the Bufeijianpi group in the 32nd week (P < 0.05, P < 0.01), and so did phosphorylation-STAT3 in Bufeiyishen group (P < 0.01) and phosphorylation-STAT3 and STAT5 in the Yiqizishen group (P < 0.05, P < 0.01). However, the aminophylline group showed no significant difference in above indicators. The three medicines regulating and tonifying lung and kidney can effectively relieve injury of lung tissues, and have long-term effect, which may be related to the regulation of JAK/ STAT signaling. Specifically, prescription tonifying lung and spleen shows good effect in reducing JAK2, STAT3 and STAT5, prescription tonifying lung and kidney shows good effect in reducing p-STAT3, and prescription tonifying Qi and kidney shows good effect in reducing p-STAT3 and p-STAT5.

Clinical study of clearing liver fire, removing dampness, strengthening slpeen and tonifying kidney methods in treating middle-aged and old woman with chronic urinary tract infection

Objective To observe the clinical effect of the treatment of clearing liver fire,removing dampness,strengthening spleen and tonifying kidney in treating middle-aged and old woman with chronic urinary tract infection.Methods 82 cases were randomly divided into two groups.42 cases in treatment group were treated with herbal prescription of clearing liver fire,removing dampness,strengthening spleen and tonifying kidney,and 40 cases in control group were treated with sensitive antibiotics.The clinical effect,safety and changes of immune index were compared between two groups.Results In the treatment group,the recent cure rate was 76.8％,the total effective rate was 90.5％ and the complete cure rate was 61.9％; while in the control group,the recent cute rate was 50％,the total effective rate 65％ and the complete cure rate 27.5％.It showed significant difference between two groups (Z=2.740,P＜0.05).There was no significant difference between two groups in hepatic and renal function,serum and urine β2-MG level before and after treatment and it showed a better clinical safety in treatment group.The level of IgA,IgG and IgM increased after the treatment in treatment group.Comparing with the control group,the results showed significant differences(t=9.1,10.5 and 11.2 respectively,P＜0.01).In treatment group the level of CD4+was increased,CD8+was decreased,and the ratio of CD4+/CD8+ was increased obviously after the treatment.The differences before and after the treatment and between groups were significant(t=13.3,8.6,P＜0.01).In control group the level of CD4+、CD8+、CD4+/CD8 +did not change significantly.The differences before and after the treatment had no statistical significance (P＞0.05).Conclusion The treatment with herbs of clearing liver fire,removing dampness,strengthening slpeen and tonifying kidney had better effect,less side effect and good research prospect. Key words: Urinary tract infection; Clearing liver fire and removing dampness, Strengthening spleen and tonifying kidney; Middle-aged and old woman

The effect of TCM method of regulating liver Qi and tonifying kidney Yin on plasma concentration of E2, P and FAS of post-surgery breast-cancer patients received endocrine therapy (Tamoxifen)

Objective The prospective study was trying to explore the altering tendency of plasma concentration of E2, P and PAS after TCM intervention for post-surgery breast cancer patient who received endocrine therapy (tamoxifen).Methods By randomization, parallel, coincidence measures and the lab. technologies of AXSYM registered at Abbott lab. in the USA, plasma concentration of E2, P and FAS were tested before and after treatment. Results The results of biochemical test of plasma E2, P and FAS concentration of the two arms showed no significance difference before and after the intervention,indicating the side effects relating to TAM had nothing to do with plasma E2, P and FAS level. Conclusion It could be predicable that there must be some other by-pass ways initiating breast cancer rather than the way directly altering the level of plasma concentration of E2, P and FAS. Key words: Breast cancer; TAM; E2; P; FAS