Tomato In India Research Articles

Processing, Value Addition and Post-Harvest Technology of Tomato: A Scoping Review on Global and Indian Perspective

India, with its favorable climatic conditions, is a major producer of tomatoes, ranking second globally. However, the tomato processing sector in India is underdeveloped, with only 1% of the produce being processed compared to developed nations such as Italy (64.02%), Spain (39.41%) and the United States (10.34%). Of the more than 180 million tonnes of tomatoes produced annually, around 40 million tonnes are processed. India's substantial tomato output, which occurs primarily in the winter, has made it a prominent participant in the global tomato processing industry. This would help the Indian market utilize tomato-based goods throughout the off-season. Lycopene, a beneficial compound, is found to be higher in processed tomato products compared to fresh tomatoes. Prominent companies in the Indian tomato processing industry include Hindustan Unilever, Nestle India, Mother Dairy, and Dabur India. Despite the potential, the sector faces challenges such as inadequate processing facilities and limited varieties suitable for processing, leading to substantial post-harvest losses. The demand for tomato-processed products in India has been increasing at 30% annually in recent years, with a rising variety of products being offered. Increased support, improved supply chain management, and adoption of advanced processing techniques could reduce wastage, add value, and enable India to become a prominent player in the global tomato processing industry. With the adoption of modern technologies such as automated sorting, grading, and cold storage facilities, the efficiency of the tomato processing industry can improve. This would reduce post-harvest losses and ensure better quality control.

Quality Assessment of South Indian Cherry Tomatoes Based on Deep and Convolutional Neural Networks

A few decades ago, quality assessment was a significant challenge in agriculture, even with tiny amounts of agricultural products. They are subject to diseases, pesticides, and environmental variables that have led to challenges to quality and safety. The quality evaluation of these items serves a crucial function in quality control and increases productivity before consumption. This paper proposes a deep neural network for solving Indian tomato diversity problems based on the assessment of their features. This study was carried out using four different categories of south Indian cherry tomatoes, i.e., spot, BER, calyx, and non-calyx. Thirty (30) relevant features were taken from an RGB-HIS color space: textural color moments, gray level co-occurrence matrix (GLCM), and defective proportion. They were then normalized in MS-EXCEL with a Min-Max function and fed into a multilayer perception deep neural network (MLP-DNN) and a convolutional neural network (CNN), respectively. The MLP-DNN was made with ten (10) hidden layers and was used with a fine-tuned convolutional neural network (CNN) of Alex net architecture with eight (8) learned layers to do grading and detection separately using MATLAB 2018a software. The results of training, testing, and validating both networks showed that they did an excellent job of solving the two-class problem (good and bad), with an overall prediction accuracy of more than 97% and a bit of loss function in both cases. However, the algorithms can be improved and employed to detect defects and sort fruits or vegetables of all kinds in the agro-industry with a larger dataset to reduce overfitting and increase validation accuracy in the future.

Erratic Rise in Tomato Prices, Status, Issues, Government Initiatives and Policy Suggestions to Manage the prices of Tomato and other Vegetables

Tomatoes, hold a paramount position in both Indian cuisine and the nation's economy. The status of tomato area and production in India and in states show its importance in Indian economy. India is leading tomato producer in the world. In India, MP and Andhra Pradesh are the largest producer of tomato in India. The erratic weather conditions, pest attack and transport bottlenecks have raised the tomato prices in June to August 2023. In this regard, the paper is to analyze the status and issues in erratic rise in tomato prices, government initiatives and Policy suggestions to manage the prices of tomato and other vegetables. In the study, it is found that the number of reasons that has led to rise in prices starting from poor prices received for tomato for rabi crop, rise in temperature in May – June and to high rainfall in July end. Government has then initiated number of needed measures to support both producers and consumers from creating the awareness, purchase of produce from surplus areas and then distribution of produce in high price areas. To prevent the abnormal price situations in tomatoes or other vegetables, there is need of a multi-pronged approach involving both short-term relief and long-term strategies. As a policy suggestion, to combat such crisis in future, a collaborative effort and coordination required among government agencies, weather departments, agricultural experts, extension machinery and farmers themselves is essential.

Molecular characterization of emerging distinct watermelon bud necrosis virus (Orthotospovirus citrullonecrosis) variant causing necrosis disease on tomato in India

Watermelon bud necrosis virus (WBNV) cause serious economic impact on the cultivation of vegetable crops in the Indian subcontinent. In this study, an Orthotospovirus that infect tomato plants with typical severe necrotic symptoms had been identified as WBNV based on N gene sequences. The complete genome sequence of WBNV isolate (22ToNV) was determined and analyzed. The nucleotide sequences of the viral RNA segments in 22ToNV exhibit <91% similarity compared to the published WBNV genome sequence. Whereas, the amino acid sequences encoded by these segments, shared identity of about 96–98.54% among the other WBNV isolates. There is a notable divergence observed specifically within the intergenic regions (IGRs) of the S and M RNAs, with a lower similarity range of 78–84%. Nucleotide sequence comparisons and phylogenetic analysis showed that 22ToNV is a unique WBNV isolate having distinct genetic differences with other known WBNV isolates reported in different geographic regions of the country.

Tomato brown rugose fruit virus associated with leaf mosaic, mottling and brown rugose patches on fruits of tomato in India

Tomato brown rugose fruit virus associated with leaf mosaic, mottling and brown rugose patches on fruits of tomato in India

Antibacterial activity of homemade Indian tomato tamarind soup (rasam) against common pathogens

Antibacterial activity of homemade Indian tomato tamarind soup (rasam) against common pathogens

Knowledge of Farmers towards Improved Tomato Production Practices in Jalpaiguri District of West Bengal

India is the second largest producer of tomato in the world after China. West Bengal is seventh largest producer of tomato in India. Present study was conducted in Jalpaiguri district of West Bengal. Present study fully relies on the primary data collected by personal interview method using a pre-tested structured interview schedule. majority of the respondents were middle age group, illiterate, majority of the respondents lived in semi-cemented house and most of the respondents were living in extended family with medium annual income and most of them belongs to SC category, medium level of social participation, scientific orientation, risk orientation, mass media exposure. Majority of the respondents had medium level of knowledge and adoption towards improved tomato production. Socio-economic characteristics like age, education, housing pattern, annual income, family type, social participation, scientific orientation, risk orientation, mass media exposure had positive and significant association with the knowledge at 0.05% of the probability. Caste and extension contacts had negative but significant association with the knowledge at 0.05% level.

First Report of Tomato Leaf Curl New Delhi Virus Infecting Several Cucurbit Plants in China

In autumn 2022, a novel and devastating viral disease affecting cucurbits emerged in Ningbo (Zhejiang province), Haimen (Jiangsu province), and Shanghai, China, causing an approximate 650-hectare infestation and resulting in nearly US$15 million in economic losses. The incidence rates of infection reached up to 72.5% on muskmelon (Cucumis melo L. ssp melo), oriental melon (Cucumis melo L. var. agrestis), pumpkin (Cucurbita moschata), luffa (Luffa acutangula), and squash (Cucurbita pepo), and were highly associated with the presence of whitefly (Bemisia tabaci). Infected plants exhibited symptoms such as dwarf stunting, reduced leaf size, leaf chlorotic patches, malformation, fruit deformation, leaf downward rolling, and yellowing (Figure 1). To identify the pathogen, forty cucurbit leaf samples were collected from Haimen (18), Ningbo (19), and Shanghai (3) and tested for cucurbits chlorotic yellows virus (CCYV), cucurbit yellow stunting disorder virus (CYSDV), and Begomovirus using RT-PCR or PCR. All samples tested negative for CCYV and CYSDV using species-specific primers; however, 29 out of 40 samples tested positive (see Supplementary Table 1) for Begomovirus using the degenerate primer pairs PA/PB (Deng et al. 1994). PCR products from seven samples, representing different regions and hosts, underwent Sanger sequencing. The nucleotide sequences of these products showed 98.2-99% identity to tomato leaf curl New Delhi virus (ToLCNDV) by BLASTn. Subsequently, the 29 positive cucurbit samples were confirmed using ToLCNDV-specific primer pairs NDVAF/NDVAR and NDVBF/NDVBR (Jyothsna et al. 2013) for DNA-A and DNA-B, respectively. The DNA-A and DNA-B genome sequences of ToLCNDV isolates from Haimen (Haimen4), Ningbo (Ningbo6), and Shanghai (Shanghai1) were obtained using the primer pairs NDVAF/NDVAR, A1961F/A2645R (covering complete DNA-A sequences), NDVBF/NDVBR, and B1613F/B2579R (covering complete DNA-B sequences，see Supplementary Table 2). No amplicon was produced with primer pairs UNA101/UNA102 and beta01/beta02 (Supplementary Table 2) for detecting Alphasatellite and Betasatellite DNAs, respectively. The complete DNA-A genome sequences (2739 bp) of Haimen 4 (accession no. OP585369), Ningbo 6 (accession no. OP585370), and Shanghai 1 (accession no. OP683993) isolates exhibited 99.5-99.6% nucleotide identity to each other, and their highest nucleotide sequence identity (99.3-99.4%) was shared with the DNA-A of ToLCNDV-Zhejiang isolate (accession no. OP356207) from tomato in Zhejiang Province, China. The complete nucleotide sequences (2693 nt) of DNA-B for Haimen 4 (accession no. OP683995), Ningbo 6 (accession no. OP683996), and Shanghai 1 (accession no. OP683994) isolates showed 99.0-99.1% identity to each other, and their highest nucleotide sequence identity (~99.1%) was shared with the DNA-B of ToLCNDV-Zhejiang isolate (accession no. OP356208).All ToLCNDV isolates from mainland China, including the Zhejiang isolate and the three isolates in this study, shared 98.3-98.7% nucleotide sequence identity and 98.2-98.4% with the DNA-A genome of the severe isolate (accession no. HM159454) from tomato in New Delhi, India, and the DNA-B genome of the India:Delhi:Cucumis:2012 isolate from cucumber in New Delhi, India, respectively. However, the genome sequence identities between mainland and Taiwan isolates (accession nos. GU180095 and GU180096) were below 93%, suggesting that mainland China isolates of ToLCNDV are more closely related to the India isolate than to the Taiwan isolate.To fulfill Koch's postulates, infectious clones of the Haimen 4 isolate were constructed and agroinfiltrated into muskmelon, oriental melon, pumpkin, luffa, and squash plants. In brief, two plasmids, containing 1.56-mer DNA-A and 1.4-mer DNA-B genome sequences, were constructed using enzyme digestion and ligation, transformed into Agrobacterium tumefaciens strain GV3101, respectively, and then co-agroinfiltrated into cucurbit plants. Initial symptoms appeared in the new leaves at 7 days post-inoculation (DPI), followed by severe leaf curling, dwarfing, stunting, reduced leaf size, and chlorotic leaf patches at 18 DPI. The presence of DNA-A and DNA-B of ToLCNDV in inoculated plants was confirmed by PCR using primer pairs A1961F/A2645R and B1613F/B2579R, respectively. Collectively, the pathogen of this emerging disease has been identified as ToLCNDV. ToLCNDV was first reported on tomato in India and is now the most predominant and economically significant disease affecting cucurbit and solanaceous crops in Southeast and East Asia, the Middle East, and the Mediterranean Basin (Moriones et al. 2017). In China, ToLCNDV was initially reported on oriental melon in Taiwan (Chang et al. 2010) and subsequently on tomato (Lycopersicon esculentum) in Zhejiang province (Li et al. 2022). To the best of our knowledge, this is the first report of ToLCNDV infecting muskmelon, pumpkin, luffa, and squash in China. Further investigations on the epidemiology of this viral disease in China are needed.

Evidence-based insecticide resistance in South American tomato leaf miner,Phthorimaea absoluta(Meyrick) under laboratory selection

The South American tomato moth, Phthorimaea absoluta (Meyrick), is one of the key pests of tomato in India. Since its report in 2014, chemical control has been the main means of tackling this pest, both in the open field and protected cultivation. Despite regular insecticidal sprays, many outbreaks were reported from major tomato-growing regions of South India during 2019-2020. A study was conducted to investigate the effect of insecticide resistance on biology, biochemical enzymes, and gene expression in various P. absoluta field populations viz., Bangalore, Kolar, Madurai, Salem, and Anantapur to commonly used insecticides such as flubendiamide, cyantraniliprole, and indoxacarb. Increased levels of insecticide resistance ratios (RR) were recorded in P. absoluta populations of different locations. A significant increase in cytochrome P450 monooxygenase (CYP/MFO) and esterase levels was noticed in the resistant population compared to susceptible one. Through molecular studies, we identified four new CYP genes viz., CYP248f (flubendiamide), CYP272c, CYP724c (cyantraniliprole), and CYP648i (indoxacarb). The expression levels of these genes significantly increased as the folds of resistance increased from G1 to G20 (generation), indicating involvement of the identified genes in insecticide resistance development in P. absoluta. In addition, the resistant populations showed decreased fecundity, increased larval development period, and adult longevity, resulting in more crop damage. The information generated in the present study thus helps in understanding the development of insecticide resistance by P. absoluta and suggests the farmers and researchers to use insecticides wisely by adopting insecticide resistance management as a strategy under integrated pest management.

Management of Postharvest Losses and Wastages in the Indian Tomato Supply Chain—A Temperature-Controlled Storage Perspective

Tomatoes are an extensively cultivated and consumed horticulture product in India. Horticulture produce undergoes a series of operations such as harvesting, storage, packaging, loading, unloading, and transportation before reaching the end customer in the food supply chain (FSC). Any inefficiencies in these operations cause postharvest losses (PHL) and affect the whole FSC. However, the focus of existing studies has been more on improving productivity than addressing PHL. Several technologies, such as cold storage and evaporative cooling, are available to address PHL, but hardly any technology has been implemented in the Indian FSC. Hence, studies need to identify technology adoption barriers and perform a feasibility analysis of the available technologies. This study addresses this gap by first identifying the cause and effect of PHL in the Indian tomato FSC, exploring different technologies to address the PHL and challenges in implementing those technologies, and finally proposing a feasible option to manage PHL. The case study approach was followed for the collection of relevant data. The findings show several reasons for PHL across the stages of the FSC, including reduced shelf life due to improper storage and long-distance transport. Based on the analysis of the available technologies, temperature-controlled storage facilities and collaboration among FSC partners are suggested as the best possible solutions to address the problem of PHL.

First Report of Tomato Leaf Curl Bangladesh Virus Associated with Leaf Yellowing Symptoms in Lablab purpureus in Bangladesh

First Report of Tomato Leaf Curl Bangladesh Virus Associated with Leaf Yellowing Symptoms in <i>Lablab purpureus</i> in Bangladesh

First report of Tomato leaf curl Karnataka virus associated with leaf curl disease of Zinnia elegans in India

Tomato leaf curl Karnataka virus (ToLCKV) is a species of begomovirus which has a circular, ssDNA monopartite genome and is transmitted by the whitefly (Bemisia tabaci). Zinnia elegans is a popular ornamental bedding plant which is grown worldwide for its beautiful flowers. A few begomovirus species have been reported to infect Z. elegans, including Ageratum yellow vein China virus in Vietnam (Li et al., 2013), and Ageratum enation virus and Zinnia leaf curl virus in India (Kumar et al., 2010; Pandey & Tiwari, 2012). In March 2019 symptoms of excessive leaf curling with mild mosaic were observed on Z. elegans plants growing in a nursery in Bhopal, central India (Fig. 1). To investigate the aetiology of the disease total DNA was extracted from leaf samples from two diseased and one asymptomatic Z. elegans plant using a plant genomic DNA purification kit (Promega, USA), following the manufacturer's instructions. A begomovirus was detected from both diseased samples (but not the asymptomatic sample) by PCR using begomovirus-specific primers: CPIT-I/CPIT-T (AM180920/AM180921) (Singh, 2005), PALIv 1978/ PARIc 496 and PALIv 722/ PALIc 1960 (Rojas et al., 1993). The expected amplicons (~800 bp, 1.2 Kb and 1.2 Kb, respectively) were purified using a PCR Clean-up System (Promega, USA) and Sanger sequenced in both directions by Bioinnovations Connecting Technologies to Life (Mumbai, India). The consensus sequence from both samples was identical and the complete viral DNA-A genome of 2761 nucleotides was deposited in GenBank (Accession No. MK965196). Sequence analysis was done by BLASTn and showed that the DNA-A genome had the highest identity (96-97%) with ToLCKV (KX219744, HM007094, LN878123, U38239); Sunflower leaf curl virus (JX678965) and 94–95% with Tomato leaf curl virus (JX524172, KX831454). Phylogenetic analysis using neighbour-joining in MEGA v.7.0 showed a close relationship with isolates of ToLCKV and relationships with other begomoviruses (Fig. 2). The disease was successfully transmitted by B. tabaci from naturally infected Z. elegans to healthy seedlings of Nicotiana tabacum, Solanum lycopersicum and Z. elegans (each 3/3), symptoms being observed 30 days post inoculation. The test plants all tested positive by PCR using the begomovirus-specific primer pairs above. Positive amplicons were sequenced and the nucleotide sequences were identical to each other and the original isolate (MK965196). The present study demonstrates that Z. elegans is a host of ToLCKV. ToLCKV is a serious threat to the production of several economically important crops in India and has been reported at an incidence of >80% on soybean, sunflower and tomato in India (Malathi et al., 2017). Recently, ToLCKV and satellite molecules have been found associated with enation leaf curl disease of another ornamental plant, Pseuderanthemum reticulatum, in India (Ashwathappa et al., 2021). The whitefly transmission study provides additional evidence that ToLCKV causes the observed disease in Z. elegans and it is possible that ornamental plants may act as a reservoir of the virus. To the best of our knowledge, this is the first report of the association of ToLCKV with leaf curl disease of Z. elegans. The authors are thankful to the Vice Chancellor of Barkatullah University, Bhopal, India, for use of the facilities and the UGC, New Delhi, India for financial support under the UGC-BSR Research Start-Up grant.

Buckeye Rot of Tomato in India: Present Status, Challenges, and Future Research Perspectives.

Tomato in India is commonly exposed to various diseases of fungal, bacterial, and viral origin, resulting in substantial yield losses (≥50%). Buckeye rot (caused by Phytophthora nicotianae var. parasitica) is among the major constraints in the successful cultivation of tomato crops in various parts of the world, including the Solan district of Himachal Pradesh state, India. The fruit rot becomes more devastating under high humidity and wet soils. Symptoms generally appear on green fruit as alternate dark- and light-brown concentric rings. The genome size of P. nicotianae var. parasitica is 82 Mb with >23,000 predicted genes. High humidity (>60%) and optimal temperatures (20 to 25°C), along with rainfall (≥10 mm), help to disperse the pathogen because the inoculum reaches the fruit through splashing rain. Sporangia germinate indirectly by producing zoospores at 20 to 25°C or directly via germ tubes at >25°C. In the absence of suitable resistant varieties, no single management practice is sufficient to keep the disease below the economic threshold level; therefore, integration of cultural and chemical methods is preferable. This article aims to focus on the etiology and management challenges of buckeye rot. We recommend innovative disease management strategies such as identification and deployment of resistant cultivars as well as spraying of synthetic chemical fungicides, biocontrol agents, and use of abiotic chemicals that induce resistance for developing sustainable crop production practices.

Purple Tomato - Importance and Scope: A Review

Fruits and vegetables are a rich sources of different nutrients, pigments, secondary metabolites and other nutritional components which are beneficial and fundamental components for human health. In recent years, the consumption of coloured fruits and vegetables has increased significantly due to their superior nutritional characteristics. In this context, purple tomato is now gaining importance due to the high content of the pigment anthocyanins. This article provides knowledge on 1) Tomato in general and anthocyanin-rich purple tomato, 2) Benefits of anthocyanins in plants and human diet, 3) Strategies for development of purple tomato, 4) Better postharvest life of purple tomatoes, 5) Purple tomato in India and 6) Health benefits of purple tomato. Purple tomato fruits provide higher nutraceutical value as compared to the classical red tomato as it combines the health benefits of the anthocyanins along with other usual and known phytochemicals as already present in tomato fruit. Due to higher anthocyanins and antioxidative properties, purple tomatoes possess anti-inflammatory, anti-microbial, anti-cancerous, cardio-protective, hepto-protective and neuroprotective effects. Regular consumption of purple tomatoes has also been found effective in the prevention of certain chronic diseases of humans as well. The use of conventional breeding and modern transgenic approach is paving the way for higher anthocyanin and other nutraceuticals in tomato fruits. Higher anthocyanin content has also imparted enhanced shelf-life and better storability to purple tomatoes. In the coming time, the availability and popularity of purple tomatoes in India will be beneficial for farmers, processing industries and human health. This will also serve as another step forward towards attaining nutritional security.

Fertilizing benefits of biogenic phosphorous nanonutrients on Solanum lycopersicum in soils with variable pH

Nanoformulations of Phosphorous (P) have recently been proposed as alternatives to P fertilizers. In this study, the fertilizing efficacies of P-based nanomaterials (NMs), nanohydroxyapatite (nHAP) and nanophosphorus (nP), were examined on Solanum lycopersicum (Pusa Rohini, Indian tomato) in growth room pot experiments. These NMs differed in their mode of synthesis, chemical composition, size and shape. Rock-phosphate (RP), phosphoric acid (PA) and di-ammonium phosphate (DAP) were included as bulk materials for comparison. Three varieties of artificial soils were included in the study, neutral (pH 7.2), acidic (pH 4.3) and basic (pH 9.8). The effects of the NMs on germination, plant growth, and P content were assessed at the 15th and 30th days after treatment. The results showed that P-based NMs enhance the overall germination and plant growth by increasing P levels in all types of soils for the tomato plants in comparison to the bulk P sources. Analysis using X-ray fluorescence revealed enhanced P content in the plants indicating the uptake of P-based NMs. Evaluation of H2O2, total phenolics and total flavonoids contents after NM treatment suggest that there is no stress caused due to the application of NMs to the plant. The results of this study indicate the beneficial role of P-based NMs as fertilizers at the early stages of plant development, which opens a scope for further investigation of underlying metabolic and molecular pathways and field trials.

First report of root rot disease on Solanum lycopersicum L. caused by Fusarium vanettenii in India

AbstractTomato (Solanum lycopersicum L.) is one of the most important and widely grown vegetable crops throughout India. Typical symptoms of root rot were observed in an experimental field of ICAR‐Indian Agricultural Research Institute, New Delhi (India), during July‐August 2019 with an incidence of 25%. Based on morphological characteristics and multi‐locus sequence‐based analyses, the pathogen was identified as Fusarium vanettenii. The pathogenicity test was also done to prove Koch's postulate. To the best of our knowledge, this is the first report of F. vanettenii causing root rot disease on tomato in India.

Association of Tomato leaf curl Karnataka virus and satellites with enation leaf curl disease of Pseuderanthemum reticulatum (Radlk.) a new ornamental host for begomovirus infecting tomato in India

Association of Tomato leaf curl Karnataka virus and satellites with enation leaf curl disease of Pseuderanthemum reticulatum (Radlk.) a new ornamental host for begomovirus infecting tomato in India

Population structure analysis and marker trait association in selected set of Indian tomato (Solanum lycopersicum L.) varieties under high temperature condition

Population structure analysis and marker trait association in selected set of Indian tomato (Solanum lycopersicum L.) varieties under high temperature condition

Probiotic characterization of bacterial strains from fermented South Indian tomato pickle and country chicken intestine having antioxidative and antiproliferative activities.

The present study aims to evaluate the potential antioxidant and antiproliferative properties of probiotic bacterial isolates Weissella cibaria p3B, Bacillus subtilis CS, and Bacillus tequilensis CL, isolated from South Indian fermented tomato pickle (homemade) and gut content of indigenous country chicken. The bacterial isolates exhibited antimicrobial activity against food-borne, human pathogenic bacteria, along with better survival under different bile and acidic conditions, hydrophobicity towards several hydrocarbons, and adherence to intestinal epithelial cells (INT-407 cells). Also, the intact cell (IC) mixture of the three species showed better DPPH, ABTS, and Fe2+ chelating activity as compared to the individual IC or cell extract (CE) activity. Among the three bacterial species, W. cibaria p3B revealed maximum antiproliferative activity against HeLa and Caco-2 cancer cells, all of which were nontoxic to INT-407 cells. Apart from being non-hemolytic, the bacterial isolates did not display any necrotic inhibition in HeLa and Caco-2 cells. The cell free supernatant (CFS) of the three bacterial isolates were tested for the production of antimicrobial peptides or bacteriocins. It found that the CFS of bacterial isolates was stable at various temperature, pH and sensitive to proteolytic enzymes confirms protenoius in nature of the antimicrobil peptides or bacteriocins. The bacterial isolates showed promising antimicrobial, antioxidant as well as antiproliferative activities with better survival ability at different pH and bile concentrations. The three bacterial isolates were able to produce potential antimicrobial peptides or bacteriocins. These results indicate better compatibility of our bacterial isolates against synthetic drugs to avoid adverse side effects and can be processed as dietary supplements against food and human pathogens. They can also provide antioxidative and antiproliferative benefits to humans and animals.

Role of viral suppressors governing asymmetric synergism between tomato-infecting begomoviruses.

Mixed viral infections are common in fields and frequently exacerbate disease severity via synergistic interactions among individual viral genomic components leading to major crop loss. Two predominant species of tomato-infecting begomoviruses, Tomato leaf curl New Delhi virus (ToLCNDV) and Tomato leaf curl Gujarat virus (ToLCGuV), are known to cause severe leaf curl disease of tomato in India. Previously, we have demonstrated asymmetric synergism between these two distinct begomovirus species during mixed infection in solanaceous hosts. In the present study, we have identified the underlying proteins that positively regulate asymmetric synergism and their effect on plant defense machinery. During co-infection, the AC2 and AV2 of ToLCGuV enhanced ToLCNDV DNA accumulation in Nicotiana benthamiana as well as in their natural host, tomato. Furthermore, we found that AC2 and AV2 of ToLCNDV and AV2 of ToLCGuV play a critical role in suppression of post transcriptional gene silencing (PTGS) machinery. Taken together, AC2 and AV2 encoded proteins of ToLCGuV are the crucial viral factors promoting asymmetric synergism with ToLCNDV. KEY POINTS: • Begomoviral suppressors play vital roles in viral synergism. • AC2 and AV2 of ToLCGuV asymmetrically enhance ToLCNDV accumulation. • AC2 and AV2 of ToLCNDV and ToLCGuV AV2 are major PTGS suppressors.