Time Tag Research Articles

The Crab pulsar seen with AquEYE at Asiago Cima Ekar observatory

We are developing fast photon-counter instruments to study the rapid variability of astrophysical sources by time tagging photon arrival times with unprecedented accuracy, making use of a Rubidium clock and GPS receiver. The first realization of such optical photon-counters, dubbed AquEYE (the Asiago Quantum Eye), was mounted in 2008 at the 182 cm Copernicus Observatory in Asiago. AquEYE observed the Crab pulsar several times and collected data of extraordinary quality that allowed us to perform accurate optical timing of the Crab pulsar and to study the pulse shape stability on a timescale from days to years with an excellent definition. Our results reinforce the evidence for decadal stability of the inclination angle between the spin and magnetic axis of the Crab pulsar. Future realizations of our instrument will make use of the Galileo Global Navigation Satellite System (GNSS) time signal.

Effects of crosslinking ratio and aging time on properties of physical and chemical cellulose gels via 1-butyl-3-methylimidazolium chloride solvent

Papers used in the field of electro-responsive applications are known as Electroactive-papers (EAPaps), consisting primarily of a cellulose. 1-Butyl-3-methylimidazolium chloride is an interesting ionic liquid that acts as an effective cellulose solvent for EAPaps due to its high solubility without chain derivatization, less chain degradation, and stability in electro-responsive applications. In our work, physical and chemical cellulose gels were fabricated and studied for the effects of various crosslinking ratios (CR) and aging time (tag), with glutaraldehyde acting as the crosslinking agent. The crosslinking reaction conversion could be increased by increasing the CR and tag; the reaction products were ketone linkages and by-product water molecules. A difference in optical properties could be observed and related to the differing amounts of ketone linkages, as confirmed by FTIR-ATR quantitative analysis. UV–visible spectra showed that the prepared samples had a maximum wavelength λmax nm, a characteristic of ketone linkages. By-product water molecules exhibited plasticizing effects, as observed by the decrease in the storage moduli (G′) at 1 day aging time. The outward migration of by-product water molecules caused a slight increase in G′ at 15 days aging time due to a closer packing. The by-product water molecules induced the H+-hopping and more disordered domains resulting in an ease in ion migration. Our paper-gels showed potential characteristics towards electro-responsive applications: less preparation time (<14 h) and stable gel properties.

AT_CHLORO, a Comprehensive Chloroplast Proteome Database with Subplastidial Localization and Curated Information on Envelope Proteins

Recent advances in the proteomics field have allowed a series of high throughput experiments to be conducted on chloroplast samples, and the data are available in several public databases. However, the accurate localization of many chloroplast proteins often remains hypothetical. This is especially true for envelope proteins. We went a step further into the knowledge of the chloroplast proteome by focusing, in the same set of experiments, on the localization of proteins in the stroma, the thylakoids, and envelope membranes. LC-MS/MS-based analyses first allowed building the AT_CHLORO database (http://www.grenoble.prabi.fr/protehome/grenoble-plant-proteomics/), a comprehensive repertoire of the 1323 proteins, identified by 10,654 unique peptide sequences, present in highly purified chloroplasts and their subfractions prepared from Arabidopsis thaliana leaves. This database also provides extensive proteomics information (peptide sequences and molecular weight, chromatographic retention times, MS/MS spectra, and spectral count) for a unique chloroplast protein accurate mass and time tag database gathering identified peptides with their respective and precise analytical coordinates, molecular weight, and retention time. We assessed the partitioning of each protein in the three chloroplast compartments by using a semiquantitative proteomics approach (spectral count). These data together with an in-depth investigation of the literature were compiled to provide accurate subplastidial localization of previously known and newly identified proteins. A unique knowledge base containing extensive information on the proteins identified in envelope fractions was thus obtained, allowing new insights into this membrane system to be revealed. Altogether, the data we obtained provide unexpected information about plastidial or subplastidial localization of some proteins that were not suspected to be associated to this membrane system. The spectral counting-based strategy was further validated as the compartmentation of well known pathways (for instance, photosynthesis and amino acid, fatty acid, or glycerolipid biosynthesis) within chloroplasts could be dissected. It also allowed revisiting the compartmentation of the chloroplast metabolism and functions.

Perturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: Lipidomics analysis of a Diabetes Antibody Standardization Program sample subset

Objectives To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach. Design and methods Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000–2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach. Results Five hundred fifty-nine lipid features differentiated ( q < 0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing individuals with recently diagnosed type 1 diabetes mellitus. Conclusions A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization.

Proteomic biomarkers in plasma that differentiate rapid and slow decline in lung function in adult cigarette smokers with chronic obstructive pulmonary disease (COPD)

Chronic obstructive pulmonary disease (COPD) is the fourth leading cause of morbidity and mortality in the United States and cigarette smoking is a primary determinant of the disease. COPD is characterized by chronic airflow limitation as measured by the forced expiratory volume in one second (FEV(1)). In this study, the plasma proteomes of 38 middle-aged or older adult smokers with mild to moderate COPD, with FEV(1) decline characterized as either rapid (RPD, n = 20) or slow or absent (SLW, n = 18), were interrogated using a comprehensive high-throughput proteomic approach, the accurate mass and time (AMT) tag technology. This technology is based upon a putative mass and time tag database (PMT), high-resolution LC separations and high mass accuracy measurements using FT-ICR MS with a 9.4-T magnetic field. The peptide and protein data were analyzed using three statistical approaches to address ambiguities related to the high proportion of missing data inherent to proteomic analysis. The RPD and SLW groups were differentiated by 55 peptides which mapped to 33 unique proteins. Twelve of the proteins have known roles in the complement or coagulation cascade and, despite an inability to adjust for some factors known to affect lung function decline, suggest potential mechanistic biomarkers associated with the rate of lung function decline in COPD. Whether these proteins are the cause or result of accelerated decline will require further research.

Accurate mass tag retention time database for urine proteome analysis by chromatography-mass spectrometry

Information about peptides and proteins in urine can be used to search for biomarkers of early stages of various diseases. The main technology currently used for identification of peptides and proteins is tandem mass spectrometry, in which peptides are identified by mass spectra of their fragmentation products. However, the presence of the fragmentation stage decreases sensitivity of analysis and increases its duration. We have developed a method for identification of human urinary proteins and peptides. This method based on the accurate mass and time tag (AMT) method does not use tandem mass spectrometry. The database of AMT tags containing more than 1381 AMT tags of peptides has been constructed. The software for database filling with AMT tags, normalizing the chromatograms, database application for identification of proteins and peptides, and their quantitative estimation has been developed. The new procedures for peptide identification by tandem mass spectra and the AMT tag database are proposed. The paper also lists novel proteins that have been identified in human urine for the first time.

Abstract SY01-04: High-end proteomics enables identification of breast cancer therapy resistance predictive proteins

Abstract SY01-04: High-end proteomics enables identification of breast cancer therapy resistance predictive proteins

Ultra Storage-Efficient Time Digitizer for Pseudorandom Single Photon Counter Implemented on a Field-Programmable Gate Array

Pseudorandom single photon counting is a novel time-resolved optical measurement method, which is advantageous over convention techniques in terms of data-acquisition speed and system cost. As a critical component of the pseudorandom single photon counter, the photon arriving time digitizer should be storage efficient for a high photon counting rate, while maintaining good time accuracy. We report an ultra storage-efficient time digitizer for a pseudorandom single photon counter in this paper, which is based on the asynchronous serial communication and can store the arriving time of every photon in 1-b memory space. In addition, a novel comb-wave modulator is proposed to achieve the dc balance required for asynchronous serial communication. Our prototype implemented on field-programmable gate arrays provides a time resolution of 400 ps. It can register up to 4.2-Giga photon arriving time tags with 1024 × 32-b memory space.

IDEAL-Q, an Automated Tool for Label-free Quantitation Analysis Using an Efficient Peptide Alignment Approach and Spectral Data Validation

In this study, we present a fully automated tool, called IDEAL-Q, for label-free quantitation analysis. It accepts raw data in the standard mzXML format as well as search results from major search engines, including Mascot, SEQUEST, and X!Tandem, as input data. To quantify as many identified peptides as possible, IDEAL-Q uses an efficient algorithm to predict the elution time of a peptide unidentified in a specific LC-MS/MS run but identified in other runs. Then, the predicted elution time is used to detect peak clusters of the assigned peptide. Detected peptide peaks are processed by statistical and computational methods and further validated by signal-to-noise ratio, charge state, and isotopic distribution criteria (SCI validation) to filter out noisy data. The performance of IDEAL-Q has been evaluated by several experiments. First, a serially diluted protein mixed with Escherichia coli lysate showed a high correlation with expected ratios and demonstrated good linearity (R2 = 0.996). Second, in a biological replicate experiment on the THP-1 cell lysate, IDEAL-Q quantified 87% (1,672 peptides) of all identified peptides, surpassing the 45.7% (909 peptides) achieved by the conventional identity-based approach, which only quantifies peptides identified in all LC-MS/MS runs. Manual validation on all 11,940 peptide ions in six replicate LC-MS/MS runs revealed that 97.8% of the peptide ions were correctly aligned, and 93.3% were correctly validated by SCI. Thus, the mean of the protein ratio, 1.00 ± 0.05, demonstrates the high accuracy of IDEAL-Q without human intervention. Finally, IDEAL-Q was applied again to the biological replicate experiment but with an additional SDS-PAGE step to show its compatibility for label-free experiments with fractionation. For flexible workflow design, IDEAL-Q supports different fractionation strategies and various normalization schemes, including multiple spiked internal standards. User-friendly interfaces are provided to facilitate convenient inspection, validation, and modification of quantitation results. In summary, IDEAL-Q is an efficient, user-friendly, and robust quantitation tool. It is available for download.

Use of models of biomacromolecule separation in AMT database generation for shotgun proteomics

Generation of a complex proteome database requires use of powerful analytical methods capable of following rapid changes in the proteome due to changing physiological and pathological states of the organism under study. One of the promising technologies with this regard is the use of so-called Accurate Mass and Time (AMT) tag peptide databases. Generation of an AMT database for a complex proteome requires combined efforts by many research groups and laboratories, but the chromatography data resulting from these efforts are tied to the particular experimental conditions and, in general, are not transferable from one platform to another. In this work, we consider an approach to solve this problem that is based on the generation of a universal scale for the chromatography data using a multiple-point normalization method. The method follows from the concept of linear correlation between chromatography data obtained over a wide range of separation parameters. The method is further tested for tryptic peptide mixtures with experimental data collected from mutual studies by different independent research groups using different separation protocols and mass spectrometry data processing tools.

Simulation of dual transponder carrier ranging measurements

The most dominant error source for microwave ranging is the frequency instability of the oscillator that generates the carrier phase signal. The oscillator noise is very difficult to filter due to its extremely low frequency. A dual transponder carrier ranging method can effectively minimize the oscillator noise by combing the reference phase and the to-and-fro measurement phase from the same single oscillator. This method does not require an accurate time tagging system, since it extracts phases on the same satellite. This paper analyzes the dual transponder carrier ranging system by simulation of the phase measurements with comprehensive error models. Both frequency domain and time domain noise transfer characteristics were simulated to compare them with dual one-way ranging. The simulation results in the two domains conformed to each other and demonstrated that a high level of accuracy can also be achieved by use of the dual transponder carrier ranging system, with relatively simple instruments.

ANALISIS EMPIRIS EFEKTIVITAS MEKANISME TRANSMISI KEBIJAKAN MONETER DI INDONESIA MELALUI JALUR EKSPEKTASI INFLASI PERIODE 1990:2-2007:1

This study used Vector Auto regression (VAR) model to analyze effectiveness of monetary policy transmission mechanism in Indonesia through Inflation Expectation Channel period 1990:2-2007:1. That effectiveness was measured by two indicators, they are: (1) : (1) how fast or how many time lag needed since the shock of monetary instruments (rSBI) until the realisation of final target of monetary policy (inflation). (2) How strong the variables of inflation expectation line response the shock of SBI interest rate and other variable. This study used secondary data issued by Bank Indonesia and BPS as well as from International Finance Statistic (IFS). The result of the study shows that response velocity of variable in Inflation Expectation Channel towards shock instrument of monetary policy (rSBI) until reach the final target or time tag about 12 quarterly or twenty six months. While impulse response function of variables in this channel to the shock instrument of monetary policy (rSBI) is quiet weak and the main channel that is inflation expectation and exchange rate are not able to explain diversity final target of monetary policy (inflation) about 33,88%, while variable of inflation expectation only able to explain diversity of inflation about 15,03%. Meanwhile, we still able economically to conclude that mechanism of monetary policy transmission through Inflation Expectation Channel is effective to reach the final target of monetary policy of Indonesia period of 1990:2-2007:1

Reference map for liquid chromatography–mass spectrometry-based quantitative proteomics

The accurate mass and time (AMT) tag strategy has been recognized as a powerful tool for high-throughput analysis in liquid chromatography–mass spectrometry (LC–MS)-based proteomics. Due to the complexity of the human proteome, this strategy requires highly accurate mass measurements for confident identifications. We have developed a method of building a reference map that allows relaxed criteria for mass errors yet delivers high confidence for peptide identifications. The samples used for generating the peptide database were produced by collecting cysteine-containing peptides from T47D cells and then fractionating the peptides using strong cationic exchange chromatography (SCX). LC–tandem mass spectrometry (MS/MS) data from the SCX fractions were combined to create a comprehensive reference map. After the reference map was built, it was possible to skip the SCX step in further proteomic analyses. We found that the reference-driven identification increases the overall throughput and proteomic coverage by identifying peptides with low intensity or complex interference. The use of the reference map also facilitates the quantitation process by allowing extraction of peptide intensities of interest and incorporating models of theoretical isotope distribution.

Development of Multifunctional Pulse Processing Device for Sensor Signal Acquisition

We developed a stand-alone multifunctional pulse processing device based on a 20 MHz and 10 bit flash analog to digital converter. This module has the functions of a peak-sensing ADC, a pulse area-sensing ADC, a time tag by a digital constant fraction discrimination method and a pulse counter. Moreover, it can be used for FADC with 50 mus waveform length for the purpose of monitoring. It has four independent channels. The external trigger and internal trigger functions are implemented and internally triggered signals which can be sent out by the trigger output connector. All of these functions are implemented in the field programmable gate array (FPGA). It operates with a single 5 V DC supply and digitized data can be directly transferred to the host computer by a 100 Mbps Ethernet port. Performance test results will be reported on which include a conventional crystal scintillator as well as liquid scintillator. The module can be used for the signal processing of the various radiation detectors for small nuclear and high energy laboratory experiments, nuclear spectroscopy and particle and radiation detection.

High Spatial and Temporal Resolution Neutron Imaging With Microchannel Plate Detectors

Special microchannel plates (MCPs) developed by Nova Scientific Inc. incorporate high efficiency neutron conversion materials into the MCP to provide a high neutron stopping power. <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">10</sup> B and <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">nat</sup> Gd have high interaction cross sections for thermal neutrons and their incorporation into MCP glass is a convenient way to make efficient MCPs for neutron detection with high spatial resolution. We have evaluated neutron event counting 2D imaging detectors using these MCPs with a cross delay line readout, cross strip readout, and a Medipix2 readout. Tests at several reactors with the cross delay line and cross strip readouts have established spatial resolution with neutrons as good as ~30 microns FWHM over a 27 mm diameter detector, with event rates approaching 1 MHz, low fixed pattern noise, event time tagging of 25 ns and intrinsic background rates of < 0.05 events cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-2</sup> sec <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> . Evaluation of neutron sensing MCP detector with Medipix2 readout (14 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) has allowed operation at high counting rates (500 MHz) with the spatial resolution limited by the 55 micron pixel size of the Medipix2 readout. We have also used the Medipix2 for centroiding of neutron events to sub pixel resolution to obtain better spatial resolution (< 15 mum) for neutrons at reduced event rates (100 kHz). Initial measurements of thermal neutron detection efficiencies give values of 20% to 25% for thermal neutrons and 45% for cold neutrons without optimization of the detection geometry. Preliminary tests with shielding and a LaBr scintillator to gate neutron detections in coincidence with gamma rays produced by neutron interactions has enabled gamma ray rejection factors of 3 times 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sup> to be achieved. Further improvements in the neutron detection efficiency and gamma ray discrimination efficiency can be gained by optimization of the geometrical and electronic configurations.

Single-photon detector operating under extremely high background photon flux conditions

We are reporting our results in research and development in the field of avalanche semiconductor single-photon detectors and their application. Our goal was a development of a solid-state photon-counting detector capable of high-precision photon arrival time tagging in extremely harsh operating conditions. The background photon flux exceeding 10 9 photons per second hitting the detector active area should not avoid the useful signal detection and recognition on the signal level of units of photons per second. This is background photon flux about two orders of magnitude higher than what the conventional solid-state photon counters accept. The detection timing resolution should be better than 100 ps and the delay stability should be on picosecond level. We have developed and tested the active quenched and gated avalanche structure on silicon providing the required features in connection with the K14 detection chips. The detector is capable of gated operation under the conditions of background photon flux of 5×10 9 photons per second. The operational detector tolerates long term exposures to the input photon flux exceeding 10 15 photons (>1 mW) per second without damage.

Extended field test on the use of visual ear tags and electronic boluses for the identification of different goat breeds in the United States1

A total of 295 goats from 4 breeds (Alpine, n = 74; Angora, n = 75; Boer-cross, n = 73; Spanish, n = 73) were used to assess the retention of 3 types of electronic ruminal boluses (B1, 20 g, n = 95; B2, 75 g, n = 100; and B3, 82 g, n = 100) according to breed and feeding conditions. Time for bolus administration, reading with a handheld reader, and animal data recording (goat identification, breed, and bolus type) were registered. Each goat was also identified with 1 flag-button plastic ear tag (4.6 g, 51 x 41 mm). Retention of boluses and ear tags was regularly monitored for 1 yr. Ruminal fluid in 5 goats from each breed and management group was obtained with an oro-ruminal probe at 2 h after feeding. Ruminal pH was measured at 24 h and at wk 1, 2, 3, and 4 and used as an indicator of feeding conditions on rumen environment. Time for bolus administration differed by bolus type (B1, 14 +/- 2 s; B2, 24 +/- 2 s; B3, 27 +/- 2 s; P < 0.05) and goat breed (Alpine, 34 +/- 3 s; Angora, 17 +/- 2 s; Boer-cross, 16 +/- 1 s; Spanish, 19 +/- 2 s; P < 0.05), although differences were due to greater times for B2 and B3 in Alpine goats. Time for bolus administration averaged 22 +/- 1 s, and overall time for bolusing, reading, and data typing was 49 +/- 1 s on average. Ruminal pH differed according to breed and feeding management (lactating Alpine, 6.50 +/- 0.07; yearling Alpine, 6.73 +/- 0.07; Angora, 6.34 +/- 0.06; Boer-cross, 6.62 +/- 0.04; Spanish, 6.32 +/- 0.08; P < 0.05), but no early bolus losses occurred; rumen pH did not differ according to bolus type (B1, 6.45 +/- 0.05; B2, 6.39 +/- 0.07; B3, 6.49 +/- 0.05; P > 0.05). At 6 mo, electronic boluses showed greater retention than ear tags (99.7 vs. 97.2%; P < 0.05). At 12 mo, bolus retention was 96.3, 100, and 97.8% for B1, B2, and B3, respectively, not differing between B1 and B3 (P = 0.562). No effect of breed and bolus type on bolus retention was detected. No goat losing, at the same time, both bolus and ear tag was observed. Ear tag retention (91.7%) was less (P < 0.05) than all types of bolus (98.1%) on average. Ear tag retention in Boer-cross (98.6%) and Alpine (96.9%) goats was greater (P < 0.05) than in Spanish (88.7%) and Angora (82.9%) and tended to differ (P = 0.095) between Spanish and Alpine. In conclusion, unlike flag-button visual ear tags and mini-boluses used here, properly designed boluses (e.g., standard bolus) met International Committee for Animal Recording and National Animal Identification System retention requirements for goat identification under US conditions and are recommended in practice.

Identification of a Putative Protein Profile Associated with Tamoxifen Therapy Resistance in Breast Cancer

Tamoxifen resistance is a major cause of death in patients with recurrent breast cancer. Current clinical factors can correctly predict therapy response in only half of the treated patients. Identification of proteins that are associated with tamoxifen resistance is a first step toward better response prediction and tailored treatment of patients. In the present study we intended to identify putative protein biomarkers indicative of tamoxifen therapy resistance in breast cancer using nano-LC coupled with FTICR MS. Comparative proteome analysis was performed on ∼5,500 pooled tumor cells (corresponding to ∼550 ng of protein lysate/analysis) obtained through laser capture microdissection (LCM) from two independently processed data sets (n = 24 and n = 27) containing both tamoxifen therapy-sensitive and therapy-resistant tumors. Peptides and proteins were identified by matching mass and elution time of newly acquired LC-MS features to information in previously generated accurate mass and time tag reference databases. A total of 17,263 unique peptides were identified that corresponded to 2,556 non-redundant proteins identified with ≥2 peptides. 1,713 overlapping proteins between the two data sets were used for further analysis. Comparative proteome analysis revealed 100 putatively differentially abundant proteins between tamoxifen-sensitive and tamoxifen-resistant tumors. The presence and relative abundance for 47 differentially abundant proteins were verified by targeted nano-LC-MS/MS in a selection of unpooled, non-microdissected discovery set tumor tissue extracts. ENPP1, EIF3E, and GNB4 were significantly associated with progression-free survival upon tamoxifen treatment for recurrent disease. Differential abundance of our top discriminating protein, extracellular matrix metalloproteinase inducer, was validated by tissue microarray in an independent patient cohort (n = 156). Extracellular matrix metalloproteinase inducer levels were higher in therapy-resistant tumors and significantly associated with an earlier tumor progression following first line tamoxifen treatment (hazard ratio, 1.87; 95% confidence interval, 1.25–2.80; p = 0.002). In summary, comparative proteomics performed on laser capture microdissection-derived breast tumor cells using nano-LC-FTICR MS technology revealed a set of putative biomarkers associated with tamoxifen therapy resistance in recurrent breast cancer.

HIGH-RESOLUTION TIMING OBSERVATIONS OF SPIN-POWERED PULSARS WITH THEAGILEGAMMA-RAY TELESCOPE

AGILE is a small gamma-ray astronomy satellite mission of the Italian Space Agency dedicated to high-energy astrophysics launched in 2007 April. Its 1 microsecond absolute time tagging capability coupled with a good sensitivity in the 30 MeV-30 GeV range, with simultaneous X-ray monitoring in the 18-60 keV band, makes it perfectly suited for the study of gamma-ray pulsars following up on the CGRO/EGRET heritage. In this paper we present the first AGILE timing results on the known gamma-ray pulsars Vela, Crab, Geminga and B 1706-44. The data were collected from 2007 July to 2008 April, exploiting the mission Science Verification Phase, the Instrument Timing Calibration and the early Observing Pointing Program. Thanks to its large field of view, AGILE collected a large number of gamma-ray photons from these pulsars (about 10,000 pulsed counts for Vela) in only few months of observations. The coupling of AGILE timing capabilities, simultaneous radio/X-ray monitoring and new tools aimed at precise photon phasing, exploiting also timing noise correction, unveiled new interesting features at sub-millisecond level in the pulsars' high-energy light-curves.

AquEYE, a single photon counting photometer for astronomy

This paper describes the results obtained so far with AquEYE, a single photon counting, fixed aperture photometer for the Asiago 182 cm telescope. AquEYE has been conceived as a prototype of a truly ‘quantum’ photometer for future Extremely Large Telescopes of 30–50 m aperture. This prototype is characterized by four independent channels equipped with single photon avalanche diodes (SPADs) as detectors. The counts from the four channels are acquired by a TDC board which has a nominal 25 ps time tagging capability. Taking into account the 35 ps jitter in the SPAD itself, the overall precision of the time tags is of the order of 50 ps. The internal oscillator is locked to an external rubidium clock; a GPS pulse per second is collected by the TDC itself to obtain a UTC reference. The maximum photon count rate which the present system can sustain is 12 MHz.