Tiliacora Triandra Research Articles

A Facile Green Synthesis at Room Temperature of Silver Nanoparticle Sensor with Leaf Extract of Tiliacora triandra

Development of reliable and eco-friendly process for synthesis of metallic nanoparticles is an important step in a practical application of nanotechnology. One of the approaches towards achieving this objective is to directly use a natural substance. In this work, we have developed a simple green method for synthesis of silver nanoparticles (AgNPs) with Tiliacora triandra leaf extract at room temperature. The synthesis process was quite fast and the silver nanoparticles were formed within minutes of the silver ions coming into contact with the leaf extract. Formation of silver nanoparticles was confirmed by surface plasmon resonance at 452 nm with a UV–visible spectrophotometer. Synthesized nanoparticles were characterized by UV–visible spectroscopy, Fourier transformed infrared spectroscopy, X-ray diffraction, X-ray energy dispersive spectrometer, selected area electron diffraction, high-resolution transmission electron spectroscopy, field emission scanning electron microscopy, dynamic light scattering and zeta potential measurements. The main benefits of this sysnthesis method are that AgNPs produced with Tiliacora triandra are not only easy to prepared but should also be less toxic than those produced by other chemical methods.

Tiliacora triandra (Colebr.) Diels leaf extract enhances spatial learning and learning flexibility, and prevents dentate gyrus neuronal damage induced by cerebral ischemia/reperfusion injury in mice.

The present study investigated the effects of a local Thai vegetable, Tiliacora triandra (Colebr.) Diels, also known as Yanang, against cerebral ischemia/reperfusion injury in mice. Thirty male ICR mice were divided into three experimental groups of BLCCAO + 10% Tween 80, BLCCAO + T. triandra 300 mg/kg, and BLCCAO + T. triandra 600 mg/kg. Cerebral ischemia/reperfusion was induced by three minutes of bilateral common carotid artery occlusion (BLCCAO) followed by 18 days of reperfusion. Leaf extract was administered orally 24 hours after arterial occlusion and continued for 18 consecutive days. Cognitive abilities were evaluated using the Morris water maze. Histological analysis was conducted in the dorsal hippocampus subregions CA1, CA3, and DG and white matter regions (the corpus callosum, internal capsule, and optic tract) using 0.1 % cresyl violet and 0.1% Luxol fast blue staining. Results showed that T. triandra leaf extract at the doses of 300 and 600 mg/kg significantly enhanced spatial learning, and learning flexibility, and prevented neuronal death in the DG of mice following ischemia/reperfusion. T. triandra leaf extract enhanced spatial learning, and learning flexibility, and prevented DG neuronal death in a mice model of cerebral ischemia/reperfusion.

The effect of green synthesized gold nanoparticles on rice germination and roots**Contribution at 5th Thailand International Nanotechnology Conference (Nano Thailand-2016), 27–29 November 2016, Nakhon Ratchasima, Thailand.

In this paper, gold nanoparticles were synthesized by means of a green approach with Tiliacora triandra leaf extracts under different conditions. No additional reducing or capping agents were employed. The gold nanoparticles were characterized using UV–visible spectrophotometry, transmission electron microscope, x-ray diffraction and Fourier transform infrared spectroscopy. Gold nanoparticles synthesized at temperature of 80 °C were further used to treat rice (Oryza sativa) grains at different concentrations (0, 10, 100, 500, 1000, 2000 mg l−1) for one week. While germination percentages were high (95–98.38%), a slight decrease in root and shoot lengths relative to the control was observed. Phytotoxicity results indicated that the plant synthesized gold nanoparticles were of minimal toxicity to rice seedlings. Increases in cell death, hydrogen peroxide formation and lipid peroxidation in roots and shoots were noted. However, these increases were not statistically significant. The overall results confirmed that Tiliacora triandra synthesized gold nanoparticles are biocompatible and can be potentially used as nanocarriers in agriculture.

Hypoglycemic Activity of Leaf Extracts from Tiliacora triandra in Normal and Streptozotocin-Induced Diabetic Rats

Background: Tiliacora triandra is a common plant found in southeast Asian countries. It is traditionally used as anti-pyrogenic agent. Objective: The present study was aimed to investigate the hypoglycemic activities leaf extracts from Tiliacora triandra (TTE) in normal and streptozotocin- induced diabetic rats. Methods: TTE was prepared and daily and orally administered at dose of 300 mg/kg b.w. to the rats for 8 weeks. The body weight and blood glucose level were measured weekly. At the end of the experiments, blood samples were collected from cardiac puncture and analyzed for serum insulin levels. The pancreatic tissues were stained by hematoxylin-eosin for histo-pathological investigations. Results: Normal and diabetic rats treated with TTE and glibenclamide tended to have an increased body weight. TTE significantly decreased the blood glucose level by 25.01 ± 19.77% in week 3 in diabetic rats and similar to that of glibenclamide group (27.01 ± 11.89%). However, the extracts slightly decreased the blood glucose level in normal rats by 9.48 ± 2.14% in week 2. TTE significantly increased serum insulin level by 21.63 ± 1.39 IU/mL in diabetic rats compared to the controls (10.63 ± 0.37 IU/mL) but not in normal rats. In additions, TTE activated the regeneration of pancreatic Islets of Langerhans in diabetic rats which in turn stimulated insulin secretion. Conclusion: TTE exhibits the hypoglycemic potential by stimulating insulin secretion from the pancreas.

Metabolite diversification by cultivation of the endophytic fungus Dothideomycete sp. in halogen containing media: Cultivation of terrestrial fungus in seawater

The endophytic fungus, Dothideomycete sp. CRI7, isolated from the terrestrial plant, Tiliacora triandra, was salt tolerant, capable of growing in the culture medium prepared from seawater; salts in seawater did not have any effects on the fungal growth. Metabolite productions of the fungus CRI7 cultivated in media prepared from seawater (MSW), prepared from deionized water supplemented with potassium bromide (MKBr) or potassium iodide (MKI), and prepared from deionized water (MDW) were investigated. It was found that the cultivation of the fungus CRI7 in MKBr and MSW enabled the fungus to produce nine new metabolites (1–9). The production of an azaphilone, austdiol (10), of the fungus CRI7 grown in MDW was 0.04g/L, which was much lower than that grown in MSW, MKBr, and MKI media which provided the yields of 0.5, 0.9, and 1.2g/L, respectively, indicating that halogen salts significantly enhanced the production of the polyketide 10. The cultivation of terrestrial fungi in media containing halogen salts could therefore be useful for the metabolite diversification by one strain-many compounds (OSMAC) approach. Moreover, the isolated polyketides had significant biosynthetic relationship, suggesting that the cultivation of fungi in halogen containing media could provide the insights into certain polyketide biosynthesis. One of the isolated compounds exhibited antibacterial activity with the MIC value of 100μg/mL.

Phytochemical and Antimicrobial Properties of Tiliacora triandra Stem Bark

Aim: Tiliacora triandra (T. triandra), a species of Menispermaceae family, has folkloric reputation for the treatment of several diseases including infectious diseases in Thailand and neighboring countries. The present study aimed at screening the stem bark of T. triandra for its phytochemical constituents and its antimicrobial potential against selected bacteria and fungi. Materials and Methods: The dried stem bark of T. triandra was extracted with methanol and qualitative phytochemical analysis was performed. The antimicrobial activity was determined by disc diffusion assay method against some bacteria and fungi. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by serial dilution method to establish the antimicrobial potential of the extract. Results: Qualitative phytochemical analysis revealed the presence of phenolics, flavonoids, terpenoids, alkaloids, saponins and cardiac glycosides. Susceptibility testing by disc diffusion assay showed significant antimicrobial activity against the tested pathogens. The highest antibacterial activity was observed against a Gram-negative bacterium Escherichia coli (E. coli) where the zones of inhibition were 15 mm and 21 mm at the potencies of 250 and 500 µg/disc, respectively. The stem bark extract also showed moderate activity against Shigella sonnei (14 mm), Shigella dysenteriae (13 mm), Agrobacterium spp (13 mm) bacteria and fungus, Aspergillus niger (14 mm) at 250 µg/disc. The results also revealed equal MIC and MBC values of 62.5ug/mL against E. coli. Conclusions: Our findings revealed that the methanol extract of T. triandra possesses potent antimicrobial activity which may be attributed to the identified phytochemical components of the plant extract.

Spray drying probiotics along with maoluang juice plus Tiliacora triandra gum for exposure to the in vitro gastrointestinal environments

Application of potential probiotic bacteria in dehydrated fruit juices could be an optional diversity for the consumers who are aware of functional beverages. This study purposed to investigate the impact of Tiliacora triandra gum on the survival of Lactobacillus casei 01 and Lactobacillus acidophilus LA5 along with maoluang juice after spray drying and their activities under simulated gut models, as compared to inulin addition. The results showed that probiotic-maoluang juice powders as immobilized by Tiliacora triandra gum and inulin similarly showed protective ability on probiotic bacteria against adverse conditions of simulated gastrointestinal (SGI) model. After 6 h incubation in SGI, the survival rates of spray dried L. casei 01 and L. acidophilus LA5 with maoluang juice and Tiliacora triandra gum or inulin were higher than the free cells, by increasing around 15–20% and 20–21%, respectively. According to the in vitro colon experiments, Tiliacora triandra gum and inulin apparently enhanced the accumulation of lactic acid, short-chain fatty acids (SCFA) and beneficial colon bacteria (i.e. lactobacilli and bifidobaccteria), whilst the levels of toxic ammonia and the populations of harmful bacteria (i.e. clostridia and fecal coliforms) were diminished. Therefore, this indicates that Tiliacora triandra gum displayed prebiotic impacts on the human colon microbiome.

Antiplasmodial activities of a Thai traditional antipyretic formulation, Bencha-Loga-Wichian: A comparative study between the roots and their substitutes, the stems

Ethnopharmacological relevanceBencha-Loga-Wichian (BLW) is a polyherbal antipyretic formulation that is comprised of Capparis micracantha, Clerodendrum indicum, Ficus racemosa, Harrisonia perforata, and Tiliacora triandra. A traditional medical textbook has documented the use of this formulation for the treatment of many types of fever, including malaria-like fever. Traditionally, BLW is composed of the root parts of those plants. However, in current practice, the stem parts are frequently substituted. Thus, this study aimed to evaluate the antiplasmodial activities of BLW and compare the efficacy between the stem and root parts. Materials and methodsBLW formulations produced from either the stem or root parts of the various constituent plants as well as the stems or roots of the individual plants were separately extracted and tested against the chloroquine-sensitive (Pf3D7) and -resistant (PfW2) strains Plasmodium falciparum using flow cytometry. The cytotoxicity against peripheral blood mononuclear cells was evaluated using the WST-8 assay to determine the selectivity index (SI). The active compounds of BLW were isolated using antiplasmodial-guided isolation and quantified using Ultra-Performance Liquid Chromatography (UPLC). ResultsThe stem and root parts of BLW and the individual plants exhibited antiplasmodial activities at the same levels with good SI values in the range of 3.55–19.74. The extracts of BLW exhibited promising antiplasmodial activity against both Pf3D7 (IC50<5µg/mL) and PfW2 (IC50=6–10µg/mL). Among the five component plants, T. triandra was the most active and exhibited an IC50<5µg/mL against both strains of parasites with SI values >10. We isolated tiliacorinine and yanangcorinine as the major active compounds (IC50<2µg/mL). However, these two compounds demonstrated cytotoxic effects (SI<1). The UPLC analysis identified these compounds in both the stem and root parts of BLW in the range of 0.57–7.66%, which correlated with the antiplasmodial activity. The concentrations of these compounds in BLW, at comparable efficacy, were much less than those at the IC50s for the single compounds alone. It suggested that synergistic interactions increased the antiplasmodial effects as well as alleviated the toxicity of the active compounds in BLW. ConclusionThis study described a promising antiplasmodial activity of BLW that had good selectivity and a toxicity-alleviating effect. The results provided scientific support for the use of this formulation for the treatment of malaria. In addition, the stem and root parts of the plants in BLW exhibited equivalent activities, which indicates the potential for the substitution of the stem parts in the formulation. Thus, we recommend additional study of the stem parts of these plants for further development on the basis of the availability and sustainability.

Chemical Constituents and in vitro anticancer activity of Tiliacora triandra leaves

Introduction: Tiliacora triandra (F. Menispermaceae) is edible and is also known for medicinal values. The leaves are a popular flavoring in Southeast Asia, especially in the northeast of Thailand. The objectives of this study were to determine the major constituents of the leaves of T. triandra, and assess their anticancer activities against human cancer cell lines. Methods: The leaves were extracted by a soxhlet apparatus with petroleum ether, dichloromethane, ethyl acetate and water. Major constituents were then purified and identified using chromatographic procedures and various spectroscopic techniques. In vitro anticancer activity tests of T. triandra extracts were performed by resazurinmicroplate assay (REMA), and tested with 3 cell lines: oral cavity cancer (KB), lung cancer (NCI-H187) and breast cancer (MCF-7) cell lines. Results: The result indicated that the main compound of T. triandra leaves was oxoanolobine. The methanol extract showed the highest cytotoxic activity against lung cancer (NCI-H187) cell line whereas the water extract exhibited the highest activity against oral cavity cancer (KB) cell line. The IC50 of oxoanolobine against the NCI-H187 cell line was 27.60 ± 4.30 µg/mL. Conclusion: T. triandra leaves contain oxoanolobine as the major constituent and have the potential of anticancer activity but are required to be investigated further.

Rheology of Yanang solution: Effect of temperature, pH and divalent cation

The main objective of this work was to study rheological properties of Yanang (Tiliacora triandra) solution; a kind of food that derived from plants is being widely used in many countries in South East Asia and East Asia. Experiments were conducted to evaluate the impact of temperature on the viscosity and then build rheological curves of products. The results showed that viscosity of Yanang solutions changes with temperature in the shear - thinning models. These solutions formed gel at low temperature and gel strength can be improved by adjusting pH and adding Ca2+ due to the presence of uronic axit residues on the backbone of Yanang gums.

Effect of three fatty acids from the leaf extract of Tiliacora triandra on P-glycoprotein function in multidrug-resistant A549RT-eto cell line.

Background:Cancer cells have the ability to develop resistance to chemotherapy drugs, which then leads to a reduced effectiveness and success of the treatment. Multidrug resistance (MDR) involves the resistance in the same cell/tissue to a diverse range of drugs of different structures. One of the characteristics of MDR is an overexpression of P-glycoprotein (P-gp), which causes the efflux of the accumulated drug out of the cell. The MDR human non-small cell lung carcinoma cell line with a high P-gp expression level (A549RT-eto) was used to investigate the bioactive compounds capable of reversing the etoposide resistance in this cell line.Materials and Methods:The leaves of Tiliacora triandra were sequentially extracted with hexane, dichloromethane, methanol and water. Only the hexane extract reduced the etoposide resistance of the A549RT-eto cell line, and was further fractionated by column chromatography using the TLC-pattern and the restoration of etoposide sensitivity as the selection criteria.Results:The obtained active fraction (F22) was found by nuclear magnetic resonance and gas chromatography-mass spectroscopy analyses to be comprised of a 49.5:19.6:30.9 (w/w/w) mixture of hexadecanoic: octadecanoic acid: (Z)-6-octadecenoic acids. This stoichiometric mixture was recreated using pure fatty acids (MSFA) and gave a similar sensitization to etoposide and enhanced the relative rate of rhodamine-123 accumulation to a similar extent as F22, supporting the action via reducing P-gp activity. In contrast, the fatty acids alone did not show this effect.Conclusion:This is the first report of the biological activity from the leaves of T. triandra as a potential source of a novel chemosensitizer.

Synthesis of Nanocrystalline Hydroxyapatite by Natural Biopolymers Based Sol-Gel Technique

Nanocrystalline hydroxyapatite (HAp) powders were successfully synthesized by natural biopolymers based sol-gel technique. The biopolymers were extracted from the leaves of Yanang (Tiliacora triandra), Krueo Ma Noy (Cissampelos pareira) and Konjac (Amorphophallus konjac). To obtain HAp powders, the prepared precursors were calcined in air at 600, 700, and 800 °C for 2 h. The phase composition of the calcined samples was studied by X-ray diffraction (XRD) technique. The XRD results confirmed the formation of HAp phase with a small trace of β-tricalcium phosphate (β-TCP). The crystalline sizes of the samples were found to be 20-50 nm as evaluated by the XRD line broadening method. TEM investigation revealed that the synthesized HAp samples consisted of nanoparticles with a particle size in the range of 50-100 nm in diameter. The corresponding selected area electron diffraction (SAED) analysis further confirmed the formation of hexagonal structure of HAp.

Antimycobacterial activity of bisbenzylisoquinoline alkaloids from Tiliacora triandra against multidrug-resistant isolates of Mycobacterium tuberculosis

Bisbenzylisoquinoline alkaloids, tiliacorinine (1), 2′-nortiliacorinine (2), and tiliacorine (3), isolated from the edible plant, Tiliacora triandra, as well as a synthetic derivative, 13′-bromo-tiliacorinine (4), were tested against 59 clinical isolates of multidrug-resistant Mycobacterium tuberculosis (MDR-MTB). The alkaloids 1–4 showed MIC values ranging from 0.7 to 6.2μg/ml, but they exhibited the MIC value at 3.1μg/ml against most MDR-MTB isolates. The present work suggests that bisbenzylisoquinoline alkaloids are potential new chemical scaffolds for antimycobacterial activity.

Phytochemical screening, antioxidant and antimutagenic activities of selected Thai edible plant extracts

Antioxidant and antimutagenic activities of plant extracts possess great potential as functional foods for cancer prevention. The aims of the current study were to evaluate antioxidant and antimutagenic activities and to study the chemical constituents of five Thai edible plant extracts. Antioxidant activity was expressed as the ability of each extract to scavenge the free radicals 1,1-diphenyl-2-picrylhydrazyl (DPPH). Antimutagenic activity was evaluated with the Ames test using Salmonella typhimurium strains TA 98, and TA 100. The results showed that Oroxylum indicum (Linn.) Kurg. and Tiliacora triandra Diels. extracts had high antioxidant activity with the EC50 values at concentrations of 12.69 ± 1.02 and 14.51 ± 0.67 mg/ml, respectively, whereasBasella alba Linn. extract had the strongest antimutagenicity with both strains of S.typhimurium with percentage of inhibition values ranging from 54.06 ± 1.52 to 86.08 ± 2.78 %. In the present study, flavonoids and phenolic compounds from the herbal extracts are proposed to be antioxidant and antimutagenic agents, respectively. The apparent antioxidant and antimutagenic activities of Thai edible plants further suggests their potential usefulness in cancer prevention. Key words: Phytochemical screening, antioxidant, antimutagenic.

A novel tricyclic polyketide and its biosynthetic precursor azaphilone derivatives from the endophytic fungus Dothideomycete sp

Azaphilone derivatives 1 and 2 and a novel tricyclic polyketide 3, together with a known azaphilone, austdiol (4), were isolated from the endophytic fungus Dothideomycete sp., which was isolated from a Thai medicinal plant, Tiliacora triandra. Compound 3 is the first polyketide having a tricyclic 6,6,6 ring system, which is similar to that of a terpenoid skeleton. The absolute configurations of stereogenic centers in 1-3 were addressed by Mosher's method and biosynthetic analogy with a known azaphilone isolated from the same fungus. Cytotoxic and antimicrobial activities of the isolated compounds were evaluated.

The Application of Local Wisdom for Production of Condiment Isan' Food Ingredient into Commercial

Problem statement: The problem of the research derives from a lack of study and understanding about the production of condiment Isan food and ingredients being introduced into commercial, even though Isan food is popular among consumers widespread both inside and outside the country. The government introduced a policy to expand trade to create an export value of Isan products. The purpose of the research is to study the local wisdom in the field of the application of the ingredients used in Isan food. In addition, the purpose is to study about the problems of applying local wisdom to produce Isan food for consumption into commercial. Approach: The fields of study are Kalasin, Khon kaen, Maha Sarakham and Roi-Et. These are the places of Isan food local culture and original application of Isan food that have been producing to the present and also popular among the consumers widespread. There are 5 types of food that are chosen to be the proposition; Kaeng Wai (Curry Wai), Kai Yang (Roasting chicken), Tom Kai Ban (Boiled chicken), Mok Lab Pla Thong (Grilled Lab Pla Thong) and Om Pla (Fish soup) from 9 restaurants. Using Qualitative Research for data gathering from the concerning document, the data will be collected from fieldwork, survey, observation, interviews and focus group discussion from a group of totally 71 informants, presenting the result by using analytical descriptive. Results: The local wisdom of application of Isan food ingredients is a herb that is easy to find in the local area. It is used for the deodorizing of meat and as a garnish to give taste and flavor to the consumer. Isan food is highlighted as spicy and salty. A Spicy taste is from hot chili and dried chili. A Salty taste can be regarded as an important deliciousness that is an identity of Isan food from pickled fish and salt. A Sour test derives from tamarinds. Ya Nang (Tiliacora triandra) is used in order to reduce uric acid of Wai (Calamus caesius Blume). Adding ginger, Kha (Languas galangal) and Lemon Grass to the main ingredients in order to be spicy and increase fragrance. Instant ingredients are used to highlight the taste and fragrance to enhance the flavor. Moreover, all herbs and vegetables that are used are good for our health. Following the local wisdom for the production of condiment Isan food ingredient is the production of day by day by estimating the quantity of using per day and using in a day in order to be fresh. The product is prepared by washing with water and cutting unnecessary parts away, using an easy kitchen application for production. The problem of using the application of local wisdom for production of condiment Isan food ingredient is the lack of available product and it is quite expensive in some seasons. Problems are encountered with low quality products, rear of labor and long period of times to produce and keep the product. Applying the application of local wisdom for production of condiment Isan food ingredient into commercial, fresh product is chosen and washed with water and unnecessary parts are cut away by using simple kitchen application in order to prepare the product. A drying technique is used by using a heated air dryer, drum dryer and freeze dryer. The research attempts to find out the result of the test by using the senses of evaluation, smell, color and taste of the food. The packaging and ticketing of the product is also important to show the ingredients, condiment, proportion and guidance of how to store the product. Conclusion/Recommendation: Applying the application of local wisdom for production of condiment Isan food ingredient into commercial is the way of using basic local wisdom in order to apply and develop the product by integrating between original production and modern technology. Both two ways can control the temperature and moisture in order to gain the most resemble of the original product. In addition, it is easy to keep in some difficult seasons, saves time and natural resources; it is easy to cook and has the prospect of being developed into industry to import and export. It is the way to bring income into the country, including enabling Isan food and culture to be recognized worldwide.

In vitro antiplasmodial activities and cytotoxicity of water extracts of Piper rostratum Roxb., Sida rhombiforia Linn. and Tiliacora triandra (Colebr.) Diels

This study aims to evaluate the in vitro antiplasmodial activity and cytotoxicity of Piper rostratum Roxb., Sida rhombiforia Linn. and Tiliacora triandra (Colebr.) Diels., herbs traditional used to treat malaria in Thailand. The water extracts of these Thai medicinal plants were tested for their antiplasmodial activity by assessing their ability to inhibit the uptake of [3H] hypoxanthine into the multidrug-resistant strain Plasmodium falciparum K1. The antiplasmodial activity was expressed by the concentration that inhibited 50% of parasite growth (IC50). Cytotoxicity of the extracts was determined on Vero cells, and the Cytotoxicity Index (CI=IC50 on Vero cells/IC50 on Plasmodium falciparum) was calculated to evaluate the safety of tested extracts. Tiliacora triandra (Colebr.) Diels. was the only one of three plants that showed the in vitro antiplasmodial activity (IC50=43.43±0.90µg/ml) with good Cytotoxicity Index (CI=5.92) whereas Piper rostratum Roxb. and Sida rhombifora Linn. did not show this activity. Further study is needed to evaluate an in vivo antiplasmodial activity of Tiliacora triandra (Colebr.) Diels. extract.

Extraction and physicochemical characterisation of polysaccharide gum from Yanang ( Tiliacora triandra) leaves

Yanang ( Tiliacora triandra) is a vegetable used in many cuisines of the northeast of Thailand and Lao People’s Democratic Republic. This paper reports the optimised extraction and some physicochemical characteristics of polysaccharide gum from Yanang leaves. The optimised extraction condition was at the leaf:water ratio of 1:6.6, 85 °C, and 100 min extraction time. The major monosaccharide constituent of Yanang gum was xylose, together with substantial amounts of other neutral sugars. The FT-IR spectra of Yanang gum were similar to that of xylan. Yanang gum exhibited shear-thinning flow behaviour and the extent of shear-thinning was concentration dependent. The mechanical spectra of Yanang gum at low concentration (0.5%) were typical of semi-diluted to concentrated solutions. However, with increasing concentration, the solution behaved like a weak gel.

Antimalarial activity of Tiliacora triandra diels against Plasmodium falciparum in vitro

AbstractPreparations from the roots of the Thai medicinal plant, Tiliacora triandra Diels, were found to have antimalarial activity against Plasmodium fakiparum in vitro. Methanol extraction yielded two alkaloidal components (water‐soluble and water‐insoluble). Only the water‐insoluble alkaloidal component showed an increase in antimalarial activity. From this component, five different alkaloids of the bisbenzyl‐isoquinoline group were isolated and identified as tiliacorine, tiliacorinine, nor‐tiliacorinine A, and two uncharacterized alkaloids G and H. Alkaloid G was found to be the most active in vitro schizontocide (ID50, 344 ng/mL) followed by nor‐tiliacorinine A and tiliacorine (ID50, 558 and 675 ng/mL, respectively).

Tilitriandrin: a new bisbenzylisoquinoline alkaloid from tiliacora triandra

Beside the known alkaloid (1S, 1′R)-tiliageine (2) and protoquercitol (4), tilitriandrine (1), a new bisbenzylisoquinoline alkaloid with diphenyl partial structure, was isolated from the aerial parts of Tiliacora triandra Diels and its structure elucidated by spectroscopic and chemical methods.