A three-story frame was designed for earthquake resistance by using post-tensioned (PT) columns and beams. Two interior connections, representing the first floor column and beam sizes, were tested under cyclic loading to evaluate the effects of energy dissipation and PT load and on the seismic behaviour. A full-scale one-story two-bay specimen frame, which was a substructure of the three-story PT building, was then built and tested. This paper presents experimental results of the connections and the frame and analytical simulations for the frame subassembly. Time-history analyses of the three-story PT building subjected to the design basis earthquake (DBE) and the maximum considered earthquake (MCE) were conducted to investigate seismic demands of the proposed frame. These tests confirmed the self-centring responses of the connections and the frame. Inelastic time history analyses of the three-story prototype building showed that the proposed frame system can achieve seismic demands under MCE level ground motions.