Thiobarbituric Acid Reactive Species Research Articles

Mechanisms of Exogenous L-Lysine in Influencing the Quality of Low-Sodium Marinated Braised Beef

During the processing of marinated braised beef, excessive sodium intake is likely to occur, which can lead to various health issues. Exogenous L-lysine (L-Lys), as an essential amino acid for the human body, has the capability to enhance the quality of low-sodium meat products. This study aimed to investigate the effects of exogenous L-Lys on the quality of low-sodium plain boiled beef and marinated braised beef, as well as its underlying mechanisms of action. Among them, the substitution rate of KCl was 60%. This study was conducted with three batches of experiments, each batch serving as an independent parallel. For low-sodium plain boiled beef, the optimal addition level of L-Lys was screened out through the research on the effects on meat quality indicators, water distribution, microstructure, and sensory evaluation. For the quality of low-sodium plain boiled beef, in terms of microstructure, the addition of L-Lys reduced muscle fiber breakage and voids, thereby improving its microstructural characteristics. Combined with quantitative descriptive analysis (QDA), the optimal level of additional L-Lys was subsequently determined to be 0.6%. It was further processed into marinated braised beef in soy sauce, and a comparative analysis was conducted with low-sodium marinated braised beef in soy sauce without L-Lys addition for shear force, meat color, thiobarbituric acid reactive substances (TBARS), and total viable count (TVC) during the storage periods of 0, 3, 6, 9, and 12 d. The results show that the redness (a*) value significantly increased within 0–12 d (p < 0.05), leading to a more stable meat color. Moreover, the addition of L-Lys significantly reduced the shear force and thiobarbituric acid reactive species (TBARS) values in the marinated braised beef (p < 0.05), thereby optimizing the tenderness of the marinated braised beef and inhibiting lipid oxidation. Although the total viable count (TVC) of the L-Lys group was higher than that of conventional low-sodium marinated braised beef in soy sauce from 9 to 12 d, both groups of products had undergone spoilage by day 12; therefore, the addition of L-Lys had no effect on the shelf life of the products. Comprehensive analysis suggested that the addition of exogenous L-Lys could optimize beef quality by enhancing hydration, improving muscle structural properties, and exerting antioxidant synergistic effects.

Boral® 500 SC (sulfentrazone) induces accumulation of heme synthesis intermediates and changes in locomotor behavior and metabolic markers in Drosophila melanogaster.

Boral® 500 SC (sulfentrazone) induces accumulation of heme synthesis intermediates and changes in locomotor behavior and metabolic markers in Drosophila melanogaster.

Assessment of the participation of sulfhydryl proteins in the glutathione peroxidase mimicry of diphenyl diselenide in the presence of thiol alkylating agent

Diphenyl diselenide (DPDS) is an organoselenium which has garnered profound interest due to its reported antioxidant viz-a-viz glutathione peroxidase (GPx) mimetic activity which is ultimately speculated to rely on oxidation of free thiols or protein thiols critical to the activities of some sulfhydryl proteins or enzymes. However, this hypothesis has not been fully established. This study was therefore carried out to investigate the antioxidant effect as well the possible involvement of proteins thiols in the in vitro GPx mimicry of DPDS. The effect of DPDS on Fe2+ (10 µM) and H2O2 (1mM)-induced lipid peroxidation and the possible involvement of thiols of cerebral sodium pump (Na+/K+-ATPase) and hepatic delta-aminolevulinic acid dehydratase (δ-ALAD) in the GPx mimicry of DPDS were evaluated in the cerebral and hepatic tissue homogenates of rat in the presence of thiol alkylating agent, iodoacetamide (IA, 2 mM). The results revealed that DPDS exerted marked (p < 0.05) inhibitory effect on Thiobarbituric acid reactive species (TBARS) production induced by Fe2+ and H2O2 in the rats hepatic and cerebral tissues and this effect was significant (p < 0.05) when compared with the control. However, IA profoundly (p < 0.05) counteracted the inhibitory action of DPDS on the TBARS production process. Furthermore, results also showed that DPDS inhibited the sulfhydryl enzymes, cerebral Na+/K+-ATPase and hepatic δ-ALAD as well as TBARS production in the same reaction system. Finally, we further established the involvement of thiols in the DPDS inhibition of sulfhydryl enzymes by assaying for pump and δ-ALAD activities in the presence of exogenous thiols [dithiothreitol (DTT) and glutathione (GSH)]. Interestingly DPDS did not inhibit the activity of these enzymes when pre-incubated or post-incubated with DTT or GSH indicating that DPDS switched from proteins/enzymes thiols to the oxidation of exogenous thiols. It is therefore apparent that the GPx mimicry/antioxidant action of DPDS is related to the loss of enzymes’ activities. Consequently, we conclude that the in vitro GPx mimicry/ antioxidant mechanism of DPDS is largely dependent on the oxidation of essential thiols of sulfydryl proteins and that DPDS could be an effective therapeutic candidate for oxidative stress-mediated conditions in which endogenous GSH level is depleted.

PHARMACOLOGICAL IMPACT OF RUTIN AND QUERCETIN IN ALCOHOL-INDUCED NEUROPATHY IN RATS

Objectives: The purpose of this research study was to assess the effect for the simultaneous administration of quercetin and rutin and their combination in neuropathic pain (NP) caused by alcohol. Methods: Rats were given ethanol (35% v/v, 10 g/kg, p.o.) for 10 weeks to induce alcoholic NP. Ingested Rutin (100 and 50 mg/kg, p.o.) and Quercetin (40 and 20 mg/kg, p.o.) individually and at their lower doses in combination (50 mg/kg Rutin and 20 mg/kg Quercetin, p.o.) to examine differences in mechanical and thermal hyperalgesia, allodynia, and histopathological parameters. Biochemical measurements of glutathione, thiobarbituric acid reactive species, and protein are used to assess the oxidative stress in alcoholic neuropathy. Results: The results of the study demonstrated that rats given alcohol for 10 weeks experienced severe mechanical and thermal hyperalgesia, allodynia, and an increased degree of oxidative stress. Lower and greater dosages of both medications alone were used to reduce the symptoms of NP brought on by alcohol. However, simultaneous low-dosage treatment of quercetin and rutin has been proven to significantly improve neurological, histopathological, and metabolic functions when compared to their individual administration. Histopathological studies, which show that nerve regeneration occurs, have provided strong support for the treatment of NP. Conclusion: Therefore, it is hypothesized that giving quercetin and rutin at the same time may help reduce NP and offer an alternate therapeutic strategy.

Ayahuasca reverses ischemic stroke-induced neuroinflammation and oxidative stress.

Ayahuasca reverses ischemic stroke-induced neuroinflammation and oxidative stress.

ACAT2 negatively modulated by FOXA2 suppresses ferroptosis to expedite the aggressive phenotypes of endometrial cancer cells.

Endometrial cancer (EC) remains a prevalent gynecological disease with a continuously rising incidence and fatality rate. Acyl coenzyme A: cholesterol acyltransferase 2 (ACAT2) has been commonly perceived as a tumor promoter in multiple human malignancies. This study was conducted to specify the role and mechanism of ACAT2 in EC, which has not been covered. The expression and prognostic significance of ACAT2 in EC samples were respectively analyzed by the ENCORI and Kaplan-Meier plotter databases. RT-qPCR and western blot examined ACAT2 and forkhead box protein A2 (FOXA2) expression in EC cells. The CCK-8 method, colony formation, and EdU staining assays detected cell proliferation. The cell cycle was detected by flow cytometry analysis. Wound healing and Transwell assays, respectively, estimated cell migration and invasion. The thiobarbituric acid reactive species (TBARS) method and BODIPY 581/591 C11 probe detected lipid peroxidation levels. FerroOrange staining estimated intracellular iron level. Western blot examined the expression of epithelial-mesenchymal transition (EMT) and ferroptosis-associated proteins. The human TFDB database predicted the binding of FOXA2 with the ACAT2 promoter, which was substantiated by ChIP and luciferase reporter assays. As a result, ACAT2 expression was increased in EC tissues and cells and associated with poor survival outcomes in EC patients. ACAT2 deletion might hinder EC cell proliferation, migration, invasion, and EMT while stimulating cell cycle arrest. Moreover, ACAT2 silencing promoted the ferroptosis of EC cells. Also, FOXA2 inactivated the transcription of ACAT2 through binding with the ACAT2 promoter. FOXA2 interference could promote the proliferation, migration, invasion, EMT, cell cycle, and inhibit the ferroptosis of ACAT2-silenced EC cells, which was partially reversed by the ferroptosis activator erastin. Conclusively, ACAT2 transcriptionally inactivated by FOXA2 might contribute to the malignant progression of EC via the inhibition of ferroptosis.

Short- and long-term sex-dependent effects of a nutritional supplement after hatching on growth, metabolism and gut microbiota in broiler chickens

ABSTRACT 1. In chicken production, a delay occurs between hatching and placement in the rearing building. This work analysed the effects of this experience on growth, metabolism, and caecal microbiota and tested whether a nutritional supplement (SUP) could mitigate these effects. 2. Chicks were placed directly in a rearing room (Control: C) or were exposed to a 24 h period without feed and water before being placed (Delayed: D). During the 24 h period, half of each group was provided with a SUP. 3. The D effect reduced body weight until d 27 in females (p = 0.017) and d 34 in males (p = 0.015). On d 1, the D group had reduced plasma triglycerides (TG) and increased uric acid (UA), total antioxidant status (TAS) and liver thiobarbituric acid reactive species in both sexes (p < 0.050). On d 34, the D group had increased UA and TAS only in females. It increased the α-diversity of microbiota in males (d 12 p = 0.036; d 34 p = 0.038) and the α-diversity in females on d 34 (p = 0.008). Changes in microbiota composition in both males and females were observed until d 34 at the genus level. 4. On d 1, the SUP increased glucose concentration in D and C group males (p = 0.001) and females (p = 0.002). The decrease in TG and increase in UA concentrations and TAS in chicks from the D group were mitigated by the SUP in females. On d 34, SUP reduced the haptoglobin-like activity in D and C group males (p = 0.041) and increased the TG concentration in C group males (p = 0.016). The SUP had little effect on the caecal microbiota. 5. Delayed placement induced long-lasting effects on growth, metabolism, and caecal microbiota composition. The effects of a nutritional supplement were variable and sex-dependent.

Effects of flavonoid extract from wonderful Kola (Buchholzia Coriacea) seed on antioxidant and liver function biomarkers of fructose fed streptozotocin induced type 2 diabetic albino rats

Wonderful kola is a wonderful herb with excellent healing powers, the seeds is fast gaining reputation because it appears to be a treatment to nearly all ailments. This study evaluates the potential hepatoprotective and antioxidant effects of oral administration of flavonoid-rich seed extract of Buchholzia coriacea seeds in fructose fed Streptozotocin (STZ)-induced diabetic rats. Twenty-five (25) rats were used for this study. They were divided into five (5) groups of five rats each; normal control, negative control (Streptozotocin (STZ)-induced diabetic), positive control (Glibenclamide), 100mg/kg of body weight seed extract and 500mg/kg of body weight seed extract. The flavonoid seed extract of the plant significantly (P&lt;0.05) reduced liver damage caused by STZ, as evidenced by the decreased serum alanine amino transferase and aspartate amino transferase activity, compared to in the group treated with STZ alone. The result of the Superoxide dismutase activity (SOS) shows more antioxidant activity than other antioxidant parameters. However, that of catalase enzyme has the lowest activity as compared to Thiobarbituric acid reactive species (TBARS) and Reduced Glutathione (GSH) activities. All were significantly (P&lt;0.05) improved following the treatments of flavonoid extract. In conclusion, the results show that seeds of Buchholzia coriacea contain a potent antioxidant and hepatoprotective effects, which can give scientific merit to the traditional use of the extract in the management of diabetes and the improvement of its complications.

Intake of Condensed Tannins (Acacia mearnsii) by Lambs in Confinement and Its Impact on Growth Performance, Rumen Environment, and Meat

This study determined whether adding condensed tannins to the diet of confined lamb improves performance, metabolism, health, and meat composition and quality. We used 24 Lacaune lambs with an average initial weight of 24.5 ± 0.24 kg. The animals were divided into 12 pens with two animals each. In the treatment group, a black wattle extract was added to the lamb concentrate (2.5 g/kg dry matter), while the control group received only the basal diet (without extract). Black wattle contained 98.6 g/kg of condensed tannin. There was no difference between the performance variables, which included weight gain, feed intake, and feed efficiency. However, the group fed on condensed tannins showed higher carcass yield and a higher percentage of linked intramuscular fat. In meat, a lower proportion of saturated fatty acids combined with higher concentrations of unsaturated fatty acids was observed in lambs of the condensed tannin group. The polyunsaturated fatty acids (PUFA) were no different; however, a higher PUFA/SFA ratio was observed in animals with condensed tannin intake, and a higher proportion of ω6 and ω3 was observed in the meat of these animals. The meat’s protein and ash content did not differ between treatments, as did the meat’s water retention capacity, color, and luminosity. Furthermore, animals that fed on condensed tannins had lower cholesterol levels and higher globulin content. The values of erythrogram variables were lower in lambs that consumed condensed tannins. More significant ruminal bacterial activity was also observed in the group receiving condensed tannins. The antioxidant effect was verified with lower thiobarbituric acid reactive species (TBARS) levels and more significant antioxidant enzyme activity. We conclude that adding condensed tannins improves carcass yield and increases the percentage of fat in the carcass, improving the fatty acid profile, which may be linked to the antioxidant effect, in addition to the health benefits of the consumer of this product.

Evaluation of the toxic potential of ethyl methanesulphonate (EMS) on Hydra vulgaris

Evaluation of the toxic potential of ethyl methanesulphonate (EMS) on Hydra vulgaris

Brivaracitam Ameliorates Increased Inflammation, Oxidative Stress, and Acetylcholinesterase Activity in Ischemic Mice

ObjectiveCerebral ischemia is a medical condition that occurs due to poor supply of blood in the brain. Reperfusion being savage further exaggerates the tissue injury causing cerebral ischemia/reperfusion injury (CI/R). CI/R is marked by an impairment in release of neurotransmitter, excitotoxicity, oxidative stress, inflammation, and neuronal apoptosis. The current study has utilized brivaracetam (BRV), a synaptic vesicle protein 2A modulator in experimental model of CI/R injury.MethodsCI/R injury was induced in Swiss Albino mice by occlusion of common carotid arteries followed by reper-fusion. Animals were assessed for learning and memory, motor coordination (Rota rod, lateral push, and inclined beam walking test), cerebral infarction, and histopathological alterations. Biochemical assessments were made for oxidative stress (thiobarbituric acid reactive species, reduced glutathione, catalase, superoxide dismutase), inflammation (tumor necrosis factor-α and interleukin-10), and acetylcholinesterase activity (AChE) in brain supernatants.ResultsCI/R animals showed impairment in learning, memory, and motor coordination, along with increase in cerebral infarction, and histopathological alterations. Furthermore, increase in brain oxidative stress, inflammation, and AChE activity were recorded in CI/R animals. Administration of BRV (10 mg/kg and 20 mg/kg; p.o.) was observed to recuperate CI/R induced impairments in behavioral, biochemical, and histopathological analysis.ConclusionIt may be concluded that BRV mediates neuroprotection during CI/R via decreasing brain oxidative stress, inflammation, and AChE activity.

Neurodegenerative biomarkers and inflammation in patients with propionic and methylmalonic acidemias: effect of L-carnitine treatment.

Propionic and methylmalonic acidemias (PAcidemia and MMAcidemia, respectively) are genetic disorders characterized by acute metabolic decompensation and neurological complications. L-carnitine (LC) is effective in reducing toxic metabolites that are related to the pathophysiology of these diseases. Therefore we investigated biomarkers of inflammation (cytokines and C-reactive protein (CRP)), neurodegeneration (BDNF, NCAM-1 and cathepsin-D) and biomolecules oxidation (sulfhydryl content and thiobarbituric acid-reactive species (TBARS)), as well as carnitine concentrations in untreated patients with PAcidemia and MMAcidemia, in patients under treatment with LC and a protein-restricted diet for until 2 years and in patients under the same treatment for more than 2 years. It was verified an increase of CRP, IL-6, IL-8, TNF-α, IL-10, NCAM-1 and cathepsin-D in untreated patients compared to controls. On the other hand, reduced levels of TNF-α, CRP, IL-10, NCAM-1 and cathepsin-D were found in plasma from treated patients, as well as increased concentrations of LC. Furthermore, oxidative biomarkers were increased in untreated patients and were normalized with the prolonged treatment with LC. In conclusion, this work shows, for the first time, that inflammatory and neurodegenerative peripheral biomarkers are increased in patients with PAcidemia and MMAcidemia and that treatment with LC is effective to protect against these alterations.

Antioxidant and anti-glycation activities of Mandevilla velutina extract and effect on parasitemia levels in Trypanosoma cruzi experimental infection: In vivo, in vitro and in silico approaches

Antioxidant and anti-glycation activities of Mandevilla velutina extract and effect on parasitemia levels in Trypanosoma cruzi experimental infection: In vivo, in vitro and in silico approaches

Phytofabrication, characterization of silver nanoparticles using Hippophae rhamnoides berries extract and their biological activities.

Fabrication of plant-based metal nanoparticles has yielded promising results, establishing this approach as viable, sustainable, and non-toxic in the biomedical sector for targeted drug delivery, diagnostic imaging, biosensing, cancer therapy, and antimicrobial treatments. The present work demonstrates the suitability of Hippophae rhamnoides berries for the instant green synthesis of silver nanoparticles to check their antioxidant, lipid peroxidation, and antimicrobial potential. The preliminary characterization of Hippophae rhamnoides-mediated AgNPs was validated by monitoring the color shift in the solution from pale yellow to reddish brown, which was further confirmed by UV-vis spectroscopy and the plasmon peaks were observed at 450 nm. Field Emission Scanning Electron Microscopy (FESEM) and X-ray diffraction (XRD) were used to evaluate the surface topography and structure of AgNPs. Herein, the antioxidant potential of synthesized AgNPs was investigated using DPPH free radical assay and the antimicrobial efficacy of similar was checked against E. coli and S. aureus by following MIC (minimum inhibitory concentration) and MBC (Minimum bactericidal concentration) assay. Along with the inhibitory percentage of lipid peroxidation was analysed by following TBARS (Thiobarbituric acid reactive species) assay. The results revealed that the AgNPs were spherical in shape with an average size distribution within the range of 23.5-28 nm and a crystalline structure. Negative zeta potential (-19.7 mV) revealed the physical stability of synthesized AgNPs as the repulsive force to prevent immediate aggregation. The bioactive functional moieties involved in reducing bulk AgNO3 into AgNPs were further validated by FTIR. TBARS was adapted to test lipid peroxidation, and Hippophae rhamnoides-mediated AgNPs showed a 79% inhibition in lipid peroxidation compared to Hippophae rhamnoides berries extract as 65%. Furthermore, the antibacterial tests showed 37 ± 0.01 mm and 35 ± 0.0132 mm, zones of inhibition against E. coli MTCC 1698 and S. aureus MTCC 3160 with MIC and MBC values of 1 mg/mL, respectively.

Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)

Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)

Beneficial effect of lupeol and metformin in mouse model of intracerebroventricular streptozotocin induced dementia.

This study examines the effectiveness of lupeol and metformin in a mouse model of dementia generated by intracerebroventricular streptozotocin (i.c.v., STZ). Dementia was induced in Swiss mice with the i.c.v. administration of STZ at a dosage of 3mg/kg on the first and third day. The assessment of dementia involved an examination of the Morris Water Maze (MWM) performance, as well as a number of biochemical and histological studies. STZ treatment resulted in significant decrease in MWM performance; various biochemical alterations (increase in brain acetyl cholinesterase (AChE) activity, thiobarbituric acid reactive species (TBARS), nitrite/nitrate, and reduction in nuclear factor erythroid 2 related factor-2 (Nrf-2), reduced glutathione (GSH) levels) and neuroinflammation [increased myeloperoxidase (MPO) activity & neutrophil infiltration]. The administration of Lupeol (50mg/kg & 100mg/kg; p.o.) and Metformin (150mg/kg & 300mg/kg; p.o.) demonstrated a considerable reduction in the behavioral, biochemical, and histological alterations produced by STZ. Low dose combination of lupeol (50mg/kg; p.o.) and Metformin (150mg/kg; p.o.) produced more pronounced effect than that of high doses of either agent alone. It is concluded that Lupeol and Metformin has shown efficacy in dementia with possible synergism between the two and can be explored as potential therapeutic agents for managing dementia of Alzheimer's disease (AD) type.

Improved Antioxidant Blood Parameters in Piglets Fed Diets Containing Solid-State Fermented Mixture of Olive Mill Stone Waste and Lathyrus clymenum Husks.

Solid-state fermentation represents a sustainable approach for the conversion of agro-industrial wastes into high-added-value feed ingredients. The present study aimed to evaluate the effects of the dietary addition of a solid-state-fermented mixture of olive mill stone waste (OMSW) and Lathyrus clymenum husks (LP) on the antioxidant blood parameters of weaned piglets. Two hundred 35-day-old weaned piglets were allotted into two groups and fed either a control (C) diet or a diet containing 50 g of OMSW-LP per kg (OMSW-LP) for 40 days. Blood samples were collected at 35 and 75 days of age to assess the free radical scavenging activity (FRSA), reduced glutathione (GSH) levels, catalase activity (CAT), protein carbonyls (CARBs), and thiobarbituric acid reactive species (TBARS). The OMSW-LP diet reduced the TBARS (p = 0.049) and CARB contents (p = 0.012) and increased the levels of FRSA (p = 0.005), GSH (p = 0.040), and CAT activity (p = 0.012) in the piglets' blood, likely due to the synergistic action of the antioxidants and bioactive compounds present in the OMSW-LP mixture. Overall, the dietary inclusion of solid-state-fermented OMSW-LP at 50 g/kg could potentially serve a bio-functional purpose since it enhanced the antioxidant blood parameters in this study, a crucial factor for the health and growth of piglets post-weaning.

5,7-dihydroxy-3′,4′,5′-trimethoxyflavone mitigates lead induced neurotoxicity in rats via its chelating, antioxidant, anti-inflammatory and monoaminergic properties

5,7-dihydroxy-3′,4′,5′-trimethoxyflavone mitigates lead induced neurotoxicity in rats via its chelating, antioxidant, anti-inflammatory and monoaminergic properties

Phytochemical composition and in vitro antioxidant and antimicrobial activities of Bersama abyssinica F. seed extracts

Medicinal plants can be potential sources of therapeutic agents. Traditional healers use a medicinal plant from Ethiopia, Bersama abyssinica Fresen, to treat various diseases. This study aimed to investigate the phytochemical components and antioxidant and antimicrobial activities of B. abyssinica seed extracts (BASE). Gas chromatography coupled to mass spectroscopy (GC–MS) analysis was used to determine the phytochemical compositions of BASE. The antioxidant activities were assessed by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, thiobarbituric acid-reactive species (TBARS) assay, ferric chloride reducing assay and hydroxyl scavenging capacity assay. Antimicrobial activity was investigated using the agar well diffusion method. Phytochemical screening showed the presence of saponins, glycosides, tannins, steroids, phenols, flavonoids, terpenoids, and alkaloids. GC–MS analysis revealed the presence of 30 volatile compounds; α-pinene (23.85%), eucalyptol (20.74%), β-pinene (5.75%), d-limonene (4.05%), and o-cymene (5.02%). DPPH-induced free radical scavenging (IC50 = 8.78), TBARS (IC50 = 0.55 µg/mL), and hydroxyl radicals’ scavenging capacities assays (IC50 = 329.23) demonstrated high antioxidant effects of BASE. Reducing power was determined based on Fe3+–Fe2+ transformation in the presence of extract. BASE was found to show promising antibacterial activity against S. aureus, E. coli, and P. aeruginosa (zone of inhibition 15.7 ± 2.5 mm, 16.0 ± 0.0 mm, and 16.7 ± 1.5 mm, respectively), but excellent antifungal activities against C. albican and M. furfur (zone of inhibition 22.0 ± 2.0 mm and 22.0 ± 4.0 mm, respectively). The seeds of B. abyssinica grown in Ethiopia possess high antioxidant potential, promising antibacterial and superior antifungal activity. Therefore, seeds of B. abyssinica provide a potential source for drug discovery.

Exploring the antioxidant potential of chalcogen-indolizines throughout in vitro assays.

Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are highly reactive molecules produced naturally by the body and by external factors. When these species are generated in excessive amounts, they can lead to oxidative stress, which in turn can cause cellular and tissue damage. This damage is known to contribute to the aging process and is associated with age-related conditions, including cardiovascular and neurodegenerative diseases. In recent years, there has been an increased interest in the development of compounds with antioxidant potential to assist in the treatment of disorders related to oxidative stress. In this way, compounds containing sulfur (S) and/or selenium (Se) have been considered promising due to the relevant role of these elements in the biosynthesis of antioxidant enzymes and essential proteins with physiological functions. In this context, studies involving heterocyclic nuclei have significantly increased, notably highlighting the indolizine nucleus, given that compounds containing this nucleus have been demonstrating considerable pharmacological properties. Thus, the objective of this research was to evaluate the in vitro antioxidant activity of eight S- and Se-derivatives containing indolizine nucleus and different substituents. The in vitro assays 1,1-diphenyl-2-picryl-hydrazil (DPPH) scavenger activity, ferric ion (Fe3+) reducing antioxidant power (FRAP), thiobarbituric acid reactive species (TBARS), and protein carbonylation (PC) were used to access the antioxidant profile of the compounds. Our findings demonstrated that all the compounds showed FRAP activity and reduced the levels of TBARS and PC in mouse brains homogenates. Some compounds were also capable of acting as DPPH scavengers. In conclusion, the present study demonstrated that eight novel organochalcogen compounds exhibit antioxidant activity.