We study the impact of thermal inflation on the formation of cosmological structures and present astrophysical observables which can be used to constrain and possibly probe the thermal inflation scenario. These are dark matter halo abundance at high redshifts, satellite galaxy abundance in the Milky Way, and fluctuation in the 21-cm radiation background before the epoch of reionization. The thermal inflation scenario leaves a characteristic signature on the matter power spectrum by boosting the amplitude at a specific wave number determined by the number of e-foldings during thermal inflation (${N}_{bc}$), and strongly suppressing the amplitude for modes at smaller scales. For a reasonable range of parameter space, one of the consequences is the suppression of minihalo formation at high redshifts and that of satellite galaxies in the Milky Way. While this effect is substantial, it is degenerate with other cosmological or astrophysical effects. The power spectrum of the 21-cm background probes this impact more directly, and its observation may be the best way to constrain the thermal inflation scenario due to the characteristic signature in the power spectrum. The Square Kilometre Array (SKA) in phase 1 (SKA1) has sensitivity large enough to achieve this goal for models with ${N}_{bc}\ensuremath{\gtrsim}26$ if a 10000-hr observation is performed. The final phase SKA, with anticipated sensitivity about an order of magnitude higher, seems more promising and will cover a wider parameter space.
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