Changes In Tension Research Articles

DNA shuffling to improve crude-water interfacial activity in biosurfactants with OmpA protein of Escherichia coli

Surfactants are molecules derived primarily from petroleum that can reduce the surface tension at interfaces. Their slow degradation is a characteristic that could cause environmental issues. This and other factors contribute to the allure of biosurfactants today. Progress has been made in this area of research, which aims to satisfy the need for effective surfactants that are not harmful to the environment. In previous studies, we demonstrated the surface tension activity of the Escherichia coli transmembrane protein OmpA. Here, we carried out DNA shuffling on ompA to improve its interfacial activity. We evaluated changes in interfacial tension when exposing mutants to a water-oil interface to identify the most promising candidates. Two mutants reached an interfacial tension value lower (9.10 mN/m and 4.24 mN/m) than the original protein OmpA (14.98 mN/m). Since predicted isoelectric point values are far from neutral pH, the charge of the protein was a crucial factor in explaining the migration of proteins towards the interface. Low molecular weight mutants did not exhibit a significant difference in their migration to the interface.

Harnessing osmotic shock for enhanced intracellular delivery of (nano)cargos

Efficient intracellular delivery of exogenous (nano)materials is critical for both research and therapeutic applications. The physicochemical properties of the cargo play a crucial role in determining internalization efficacy. Consequently, significant research efforts are focused on developing innovative and effective methodologies to optimize (nano)material delivery.In this study, we utilized osmotic shock to enhance (nano)cargos internalization. We examined the effects of hypotonic/hypertonic shock on both primary and cell lines, assessing parameters such as cell viability, cell volume, membrane tension changes, and particle uptake. Our results indicate that short-lived osmotic shock does not harm cells. Hypotonic shock induced temporary shape changes lasting up to 5 min, followed by a 15-minute recovery period. Importantly, hypotonic shock increased the uptake of 100-nm and 500-nm particles by ∼ 3- and ∼ 5-fold, respectively, compared to isotonic conditions. In contrast, the hypertonic shock did not impact cell behavior or particle uptake.Notably, the internalization mechanisms triggered by osmotic shock operate independently of active endocytic pathways, making hypotonic stimulation particularly beneficial for hard-to-treat cells. When primary fibroblasts derived from amyotrophic lateral sclerosis (ALS)-patients were exposed to hypotonic shock in the presence of the therapeutic cargo icerguastat, there was an increased expression of miR-106b-5p compared to isotonic conditions.In conclusion, osmotic shock presents a promising strategy for improving drug delivery within cells and, potentially, in tissues such as muscles or skin, where localized drug administration is preferred.

Searching for the Causes of Chest-Falsetto Register Breaks under Anechoic Conditions

The origin of the breaks between chest and falsetto registers has remained one of the most controversial themes in voice science. The search for the causes of register breaks is reminiscent of a detective story. Classically, the breaks have been thought to be a consequence of a sudden change in laryngeal muscle activity causing a sudden change of vocal fold tension and frequency of oscillation. Experiments with excised human larynges showed, however, that sudden chest-falsetto breaks can spontaneously occur also when the vocal fold tension is changed smoothly (1). An alternative theoretical explanation of such phenomena emerged in the 1990s with the introduction of the theory of nonlinear dynamics to voice science. This theory showed that the phonatory system behaves like a nonlinear dynamic oscillator, where the chest and falsetto registers can be viewed as two possible ways of oscillation (attractors) that are inherent to the vocal apparatus (1). Voice breaks can then be recognized as “bifurcation events“ in which the oscillator suddenly changes the vibratory regime by shifting from one attractor to another. Whether the oscillation is in falsetto or chest register does not necessarily depend only on the laryngeal adjustment but also on other factors. Tiny changes in these factors (including, e.g., subglottal pressure, airflow, but also acoustic resonances in the breathing airways), can cause the oscillation to suddenly change from one register to another.

Development of 3D-printed braces protocol for juvenile idiopathic scoliosis: From the torso measurement and mechanical properties to preliminary experience on fitting

Background: There has been no standard for a rapid prototyping process in manufacturing of the 3D-printed brace with respect to its design, biomechanical properties, procedures, and brace adjustment and fitting. Objective: Objective of this study was to develop the 3D-printed brace protocol for juvenile idiopathic scoliosis. Methods: With the help of the Torso Measurement Frame, the child stands still and is positioned with 1 of 2 manipulation techniques by the physician. Although the patient is in the corrected position, a hand-held scanner registers the three-dimentional (3D) torso, and then the brace is designed using computer aided design (CAD) model. Finite element analysis is used to determine the minimum required thickness of the brace. Acrylonitrile Butadiene Styrene (ABS) material (thermoplastic polymers) and DuraForm PA plastic (Nylon) material were printed by 2 different systems, respectively, and were tested using standard tensile test procedures. Results: The minimum thickness of the brace should be 2 mm to maintain a standard structural factor of safety twice that of the material failure strength. The strain in elongation was less for the ABS compared to the 3D-printed Nylon (6% vs. 14%), and the tensile strength was reduced more for ABS as compared to Nylon (42% vs. 14%). The ABS brace could not withstand changes in tension and cracked when drilled into, heat flared, or donned tightly. However, the Nylon brace improved strength and eliminated the vulnerable aspects. Conclusions: The Nylon material for the 3D-printed brace improved ease of adjustments in trimming, drilling, and heat modifications for better fit and function.

Dynamic wireless sensor network-based structural health monitoring system for retractable roof structure

Retractable roof structure (RRS) works optimally under varying weather, but is prone to damage due to its complexity, requiring monitoring. Given RRS's large scale, wireless sensor networks (WSNs) are preferred, with dynamic WSNs better adapted to its motion than static ones. A tree-type network topology was designed for dynamic WSN, enabling fast self-routing. It is divided into subnets, each with a relay node as the parent and subordinate relay and sensor nodes as children. Network address reassignment method was proposed to sustain WSN communication during motion. This method involves operations of subnet affiliation change and relay node replacement, the former adjusts the hierarchy between relay nodes across different subnets while preserving parent-child relation within each subnet, and the latter swaps the roles of relay and sensor nodes within one subnet to form a new subnet. Basic communication module with processing, communication, and power management units was used to connect nodes. Dynamic WSN-based structural health monitoring (SHM) system with 372 sensors was developed for the Textile City Sports Center Gymnasium. Mean errors between simulated and measured results were 10.4 % for displacement, 13.6 % for stress during construction, and 13.3 % for tension during service, indicating good agreement. This suggests that the SHM system operated well in both phases. Maximal stress and displacement after unloading were 215.9 MPa and 549 mm, below allowable limits. Monitoring results revealed temperature field non-uniformity with a 9 °C maximal difference, and strong temperature-stress correlation, with Pearson coefficients of 0.971, 0.973, and 0.955 for tie rod, chord, and web. RRS movement significantly impacted midspan tie rods in long main trusses, with a mean tension change exceeding 1000 kN. The research methods and conclusions offer valuable references for developing and applying RRS's SHM system.

Enhancing postural stability in musculoskeletal quadrupedal locomotion through tension feedback for CPG-based controller

A central pattern generator (CPG)-based controller enhances the adaptability of quadrupedal locomotion, for example, by controlling the trunk posture. The conventional CPG-based controllers with attitude control often utilized the posture angle as feedback information. However, if the robot's body is as soft as a musculoskeletal structure, it can detect the over-tilting of the trunk based on proprioceptive information of the muscles. In general, proprioceptive information such as muscle tension changes more rapidly than posture angle information. Therefore, a feedback loop based on proprioceptive information has great potential to respond to sudden disturbances that occur during locomotion over uneven terrain. In this research, we proposed a CPG-based controller utilizing the tension of soft pneumatic artificial muscles (PAMs). Musculoskeletal quadruped robots driven by PAMs are so soft, which prevents over-tilting of the trunk because the soft leg acts like a suspension. In addition, tension, one of the proprioceptive information of PAMs, exhibits high sensitivity to changes in trunk posture because the soft body's motion easily is affected by over-tilting of the trunk. To validate the efficacy of the proposed controller, we conducted numerical simulations with a simple quadruped model and experiments with a musculoskeletal quadruped robot. As a result, the tension feedback is not effective for posture stabilization on flat terrain whereas it is effective on uneven terrain. Moreover, the tension feedback improved the running velocity over uneven terrain. These results will enhance the locomotion capability of musculoskeletal quadruped robots, advancing their practical application.

Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles.

We demonstrate rapid membrane mixing between GUVs of pure lipid compositions and membrane vesicles (MVs) isolated from the plasma membrane of Vero cells, resulting in the transfer of native lipids and proteins to the GUVs. The steps involved in the membrane mixing are docking followed by membrane fusion. We show that positively charged lipids of the GUVs are essential for the docking, and the native membrane components of MVs drive the fusion. The interleaflet and lateral asymmetry and a change in the membrane tension upon the membrane mixing trigger membrane invagination. We detected outward and inward invagination sites at the rim of the GUVs within 10-40 min of the membrane mixing. The extent of the invaginations depends on the cholesterol and sphingomyelin (SM) contents in the GUVs. Cholesterol content above a critical concentration disfavors membrane invaginations, and the SM lipid is an essential molecular factor for membrane invagination.

Combination of Milk Polar Lipids and Casein Hydrolysate as a Healthy Emulsifier for Ice Cream.

The demand for healthy ingredients in food products including ice cream, is continuously increasing. The potential of a combination of milk polar lipids (MPL) and casein hydrolysate (CH) to replace synthetic emulsifiers such as diacetyl tartaric acid esters of monoglycerides (DATEM), in ice cream production was investigated. Changes in particle size, emulsion stability, and interfacial tension of model emulsions (milk protein, casein:whey=8:2, w/v) were analyzed after the addition of MPL, CH, and their combination (MPL+CH). The use of MPL+CH reduced interfacial tension and increased αs- and β-casein displacement from the surface of cream layers compared to the addition of MPL alone. The addition of MPL+CH improved ice cream overrun to levels comparable to those of control ice cream containing DATEM (0.3%, w/v), without adversely affecting melt rate or microstructure. Confocal laser scanning microscopy revealed that ice cream prepared with MPL+CH formed a thick protein and coalesced fat layer on the surface of air cells that might help enhance overrun. These findings suggest that the combination of MPL (0.3%, w/v) and CH (0.03%, w/v) can be used as a potential emulsifier alternative to replace chemically synthesized emulsifiers such as DATEM.

КОМП'ЮТЕРНИЙ МОДУЛЬ ДЛЯ АНІМАЦІЇ РУХУ ШАТУННО-ПОВЗУНКОВОЇ ГРУПИ ПРОГРАМИ KDAM ПРИ ВИЗНАЧЕННЯ КІНЕМАТИЧНИХ ТА ДИНАМІЧНИХ ПАРАМЕТРІВ

The development of new schemes of the thread feeding system requires an operational assessment of the value of the tension in front of the working zone. The objective function in the tasks of optimizing technological processes is the minimum necessary tension. The development of special computer programs for determining the tension in the working area makes it possible to determine the necessary technological parameters. The variable parameter in the objective function is the sum of the angles of coverage of the working bodies by the thread. The use of a computer program allows you to determine the tension and change in relative tension in the filling zones of technological machines, which allows you to optimize the shape of the thread supply line even at the stage of designing the technological process. A computer module was developed for the animation of the movement of the connecting rod-slider group, which is a component of the KDAM program for determining the kinematic and dynamic parameters of the connecting rod-slider group of the mechanisms of textile and light industry machines, which allows at the preliminary design stage to visually evaluate the behavior of individual links of the connecting rod mechanism of the slider group and determine the coordinates, projections of velocities and accelerations of the center of mass of the connecting rod, the slider and the points of attachment of the links of the Asura groups on the connecting rod. The KDAM program is a structural element of a computer software complex for assessing the intensity of technological processes in the light and textile industry when determining the change in the relative tension of the thread in the filling zones on the technological equipment. Kinematic and dynamic analysis of flat mechanisms plays a significant role in the design of new mechanisms and modernization of existing ones. The results obtained during these studies can be used to calculate the strength of individual links, their inertial characteristics, optimization of structural parameters of mechanisms, and minimization of consumed energy. The computer module of the KDAM program for determining the kinematic and dynamic parameters of the connecting rod-slider group of mechanisms of light industry machines allows you to determine the coordinates, projections of velocities and accelerations of the center of mass of the connecting rod, slider and the point of attachment of the links of the Asura groups on the connecting rod. During the dynamic analysis of the operation of the connecting rod-slider group, the projections of the full reaction in the joints between the crank and the connecting rod, between the connecting rod and the slider, and between the slider and the fixed guide are determined.

Endometriosis development in relation to hypoxia: a murine model study.

Endometriosis, due to its ambiguous symptoms, still remains one of the most difficult female diseases to treat, with an average diagnosis time of 7-9years. The changing level of hypoxia plays an important role in a healthy endometrium during menstruation and an elevated expression of the hypoxia-inducible factor 1-alpha (HIF-1α) has been demonstrated in ectopic endometria. HIF-1α mediates the induction of proangiogenic factors and the development of angiogenesis is a critical step in the establishment and pathogenesis of endometriosis. Although the inhibition of angiogenesis has been proposed as one of the actionable therapeutic modalities, vascular normalization and re-oxygenation may become a possible new approach for therapeutic intervention. Our goal was to investigate whether a selected murine model of endometriosis would be suitable for future studies on new methods for treating endometriosis. Non-invasive, high-resolution ultrasound-monitored observation was selected as the preclinical approach to obtain imaging of the presence and volume of the endometriotic-like lesions. The EF5 (2-(2-Nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide) compound that selectively binds to reduced proteins in hypoxic cells was used for hypoxia detection. The expression of Pten and other crucial genes linking endometriosis and hypoxia were also assessed. Using EF5, a pentafluorinated derivative of the 2-nitroimidazole that is metabolically reduced by oxygen-inhibitable nitroreductase, we confirmed that hypoxia did develop in the selected model and was detected in uterine and ectopic endometriotic lesions. Moreover, the changes in oxygen tension also influenced the expression level of significant genes related to endometriosis, like Pten, Trp53, Hif1a, Epas1, and Vegfa. Their strong modulation evidenced here is indicative of model reliability. Using high-resolution ultrasound-based imaging, we present a non-invasive method of visualization that enables the detection and observation of lesion evolution throughout the duration of the experiment, which is fundamental for further preclinical studies and treatment evaluation. The selected model and method of visualization appear to be suitable for the study of new treatment strategies based on hypoxia alleviation and blood flow restoration.

Properties of wood shear walls connected by wood nails

The performance of a wood wall connected by wood nails and twist nails was tested. It was found that during the monotonic load testing, the wood nails connected to the shear panel and stud at the column bottom broke when the deformation reached 12.1 mm. However, the primary failure mode was that the twist nails bent and the head cap became dented in the shear panel. For reciprocating loads, it was found that the wood nails experienced local fracture on the tensile side. The skeleton curve of the wood wall connected by twist nails showed a good trend of bearing capacity changes in both tension and compression sides. Based on the properties of the standard wood wall, a new T-shaped fastener was designed for the midply wood wall. The lateral performance of the reinforced standard wood shear wall and midply wood shear wall were tested. By adding T-shaped pull-up fasteners, sandwich wall structure and double row nails, the strength, stiffness, and ductility of the midply wood wall were substantially better than those of standard walls connected with twist nails using conventional pull-up fasteners.

Glucose deprivation impairs hypoxia-inducible factor-1α synthesis

Hypoxia-inducible factors (HIFs) are key transcriptional mediators of the hypoxic response and are implicated in oncogenesis. HIFα is regulated by a well-characterized, oxygen-dependent degradation pathway involving the von Hippel Lindau (VHL) tumor suppressor protein. However, comparatively little is known about HIFα regulation at the translational level, particularly under cellular stress. There is evidence that HIFα expression not only responds to changes in oxygen tension, but also nutrient availability. In this study, we monitored global translation rates, ATP levels and HIF1α synthesis rates in response to glucose starvation or glycolysis inhibition. We found that both global and HIF1α-specific translation rates decline under glucose deprivation that is concomitant with ATP reduction. These results are in contrast with previous reports showing preferential HIF1α synthesis despite global translation suppression under hypoxia and suggest that a glucose requirement in cellular metabolism is associated with HIF1α translation.

Programmable Lipid Bilayer Tension-Control Apparatus for Quantitative Mechanobiology.

The biological membrane is not just a platform for information processing but also a field of mechanics. The lipid bilayer that constitutes the membrane is an elastic body, generating stress upon deformation, while the membrane protein embedded therein deforms the bilayer through structural changes. Among membrane-protein interplays, various channel species act as tension-current converters for signal transduction, serving as elementary processes in mechanobiology. However, in situ studies in chaotically complex cell membranes are challenging, and characterizing the tension dependency of mechanosensitive channels remains semiquantitative owing to technical limitations. Here, we developed a programmable membrane tension-control apparatus on a lipid bilayer system. This synthetic membrane system [contact bubble bilayer (CBB)] uses pressure to drive bilayer tension changes via the Young-Laplace principle, whereas absolute bilayer tension is monitored in real-time through image analysis of the bubble geometry via the Young principle. Consequently, the mechanical nature of the system permits the implementation of closed-loop feedback control of bilayer tension (tension-clamp CBB), maintaining a constant tension for minutes and allowing stepwise tension changes within a hundred milliseconds in the tension range of 0.8 to 15 mN·m-1. We verified the system performance by examining the single-channel behavior of tension-dependent KcsA and TREK-1 potassium channels under scheduled tension time courses prescribed via visual interfaces. The result revealed steady-state activity and dynamic responses to the step tension changes, which are essential to the biophysical characterization of the channels. The apparatus explores a frontier for quantitative mechanobiology studies and promotes the development of a tension-operating experimental robot.

Impact of the Interfacial Kapitza Resistance on Colloidal Thermophoresis.

Thermal gradients impart thermophoretic forces on colloidal particles, pushing colloids toward cold or hot regions, a phenomenon called thermophoresis. Current theoretical approaches relate the Soret coefficient to local changes in the interfacial tension around the colloid, which lead to fluid flow around the colloid surface. The Kapitza resistance, a key variable in the description of interfacial heat transport, is an experimentally accessible property that modifies interfacial thermal fields. Here, we introduce a theoretical approach that describes colloid thermophoretic forces by incorporating explicitly Kapitza resistance effects. Our formulation can be used to monitor the dependence of thermophoresis on interfacial thermal resistance. We show that the resistance modifies the thermal field around the colloids and identify experimental conditions where the Kapitza resistance influences the thermophoretic forces. We validate our theoretical approach by implementing a nonequilibrium molecular dynamics model of a colloid suspended in a solvent.

Selenium nanoparticles stabilized with chitosan for fortifying dairy products

Relevance. One solution to the problem of selenium deficiency is the enrichment of socially important food products, in particular dairy products, with bioavailable forms of selenium. Such forms include selenium nanoparticles. The aim of the work is to develop a dairy product enriched with selenium nanoparticles stabilized with chitosan.Methods. According to dynamic light scattering spectroscopy, a sample of selenium nanoparticles stabilized with chitosan has a monomodal size distribution with an average hydrodynamic particle radius of 25 nm.Results. Quantum chemical modeling of selenium nanoparticles stabilized by chitosan has revealed that the most energetically favorable interaction is the interaction of the surface of selenium nanoparticles with the hydroxo group attached to the C3 glucosamine residue of chitosan. A study was conducted of the influence of technological parameters on the stability of selenium nanoparticles stabilized with chitosan. It was found that increasing the exposure time leads to an increase in the average hydrodynamic radius of selenium nanoparticles stabilized by chitosan. In the case of pH, an inverse relationship is observed: particles with the largest average hydrodynamic radius are found in samples with an acidic environment (pH ˂ 5). As part of a study of the influence of technological parameters on the stability of selenium nanoparticles stabilized by chitosan, it was found that selenium nanoparticles stabilized by chitosan can be used as a source of selenium for food products that have a neutral pH, but can be subjected to heat treatment at temperatures above 70 °C in for 5–15 minutes, in particular pasteurized milk. A study of pasteurized milk fortified with selenium nanoparticles stabilized by chitosan showed that there were no significant changes in titratable acidity, surface tension and pH of milk, as well as the average hydrodynamic radius of casein micelles after milk fortification. The value of antioxidant activity increases by 0.88% — from 6.50 to 7.38%.

A force-density framework for flexible multi-body dynamic analysis of clustered tensegrity structures

This paper develops a versatile and effective force-density framework for the flexible multi-body dynamic analysis of clustered tensegrity structures. In this framework, the force density is selected as the basic variable instead of force, and the clustered tensegrity structure is mathematically described in a vector and matrix form, encompassing topology, geometry, material, and force properties. A non-negative variable is defined as an indicator of the member stress state, and a complementary function is constructed to address the discontinuity issues that arise from the unidirectional axial stiffness of cables. Dynamic formulas are established within this force-density framework, with nodal coordinates selected as generalized parameters and formulations constructed in a matrix form. A complementary framework is established as an alternative for solving the dynamic equations, transforming the isolated steps of Newton’s iteration and cable state judgment (slack or tension) into a unified one, bringing more potential for improving solving efficiency. Numerical simulations are carried out to validate the approach, demonstrating that it effectively reveals the dynamic oscillation, tension changes, and cable slack behavior of clustered tensegrity structures during shape control. Comparative studies highlight the advantage of computational efficiency. The method proposed in this paper provides a robust mathematical model for studying clustered tensegrity structures, particularly regarding the shape control of deployable, active, and intelligent structures, aiding in understanding dynamic oscillation, tension changes, and cable slack behavior during their deformation. The methods can also be applied to cable net structures and other prestressed pin-jointed systems.

Role of capillary pressure on carbon dioxide sequestration: mathematical analysis

ABSTRACT Geological disposal of CO2 after capturing the gas from large emitting sources is one of the safest and most prominent methods to eliminate CO2 from the atmosphere and mitigate climate change. The heterogeneities in porosity, permeability and capillary pressure in various underground formations impact gas distribution, affecting their storage capacity. Therefore, a numerical model is developed to study the plume migration under various physical heterogeneities by upscaling the variation in capillary pressure. Accordingly, homogeneous, heterogeneous and layered formations have been considered. The influence of capillary pressure and field-scale heterogeneities, such as porosity and permeability, is incorporated using the Leverett J function. The impact of variations in capillary pressure on gas migration is calibrated in different formations for ten years of injection. The analysis project that plume evolution in different formations highly deviates and a plume length of 3410 m is obtained for layered formation. A sensitivity analysis was conducted to understand the influence of interfacial tension in formations with differing heterogeneity. The change in interfacial tension impacts the average saturation of CO2, ranging from 0.287 to 0.155 in heterogeneous aquifers. The plume migration decreases in layered and increases in homogenous and heterogeneous media with decreased interfacial tension.

Advances in mechanotransduction and sonobiology: effects of audible acoustic waves and low-vibration stimulations on mammalian cells

AbstractIn recent decades, research on mechanotransduction has advanced considerably, focusing on the effects of audible acoustic waves (AAWs) and low-vibration stimulation (LVS), which has propelled the field of sonobiology forward. Taken together, the current evidence demonstrates the influence of these biosignals on key cellular processes, such as growth, differentiation and migration in mammalian cells, emphasizing the determining role of specific physical parameters during stimulation, such as frequency, sound pressure level/amplitude and exposure time. These mechanical waves interact with various cellular elements, including ion channels, primary cilia, cell–cell adhesion receptors, cell–matrix and extracellular matrix proteins, and focal adhesion complexes. These components connect with the cytoskeletal fibre network, enabling the transmission of mechanical stimuli towards the nucleus. The nucleus, in turn, linked to the cytoskeleton via the linkers of the nucleoskeleton and cytoskeleton complex, acts as a mechanosensitive centre, not only responding to changes in cytoskeletal stiffness and nuclear tension but also regulating gene expression through the transcriptional co-activator YAP/TAZ and interactions between chromatin and the nuclear envelope. This intricate chain of mechanisms highlights the potential of sonobiology in various fields, including dentistry, regenerative medicine, tissue engineering and cancer research. However, progress in these fields requires the establishment of standardized measurement methodologies and biocompatible experimental setups to ensure the reproducibility of results.

Radiological and biomechanical evaluation of the ulnar nerve after the percutaneous application of the medial K-wire in the extended position on supracondylar humerus fractures: Does the postoperative long arm splint position matter?

Our study aimed to evaluate and compare the changes in ulnar nerve tension and strain at different elbow positions radiologically and mechanically before and after applying the medial K-wire on the supracondylar humerus fracture cadaver model. We used ten fresh frozen cadaver upper extremity specimens to measure strain and tension on the ulnar nerve in 3 different elbow positions: elbow full extension, elbow flexion-forearm supination, and elbow flexion-forearm pronation. We employed Shear wave elastography (Siemens Acuson S3000 USG, 9L4 linear probe) and a microstrain gauge (Microstrain, Inc., Burlington) to obtain our measurements. Minimum, maximum and mean stress and strain values on the nerve and its surroundings were measured and compared statistically. The mean values of elbows with full extension are statistically lower than those in elbows with 90° flexion-forearm supination and those with 90° flexion-forearm pronation positions. Statistical evaluations were performed between all of the groups. Elbow 90° flexion-forearm pronation, both minimum and maximum and mean values were statistically higher in the group, including the specimens with Kirschner applied. The mean values in the elbow full extension and elbow 90° flexion-forearm supination positions were statistically similar in the specimens with and without the K-wire applied. Despite the numerous techniques described in the literature, there is no absolute technical method to prevent ulnar nerve damage. K-wire application to the medial epicondyle with the elbow in a slightly extended position is a technique that can be applied to reduce the risk of ulnar nerve paralysis. However, it has been reported that ulnar nerve damage can be observed in cases where a splint is placed in the 90° flexion position. We hypothesize that the position of the elbow joint in the postoperative period may contribute to ulnar nerve paralysis due to soft tissue tension and strain and as a result of changing the balance of the surrounding tissues. Our findings suggest that the long arm splint applied in elbow 90° flexion and forearm pronation position should not be preferred in the postoperative period. The maximum strain values obtained in the elbow full extension were lower, suggesting that it would be appropriate to stabilize the elbow in the extension position as much as possible postoperatively. Level of evidence: Level V.

Membrane Tension Regulation is Required for Wound Repair.

Disruptions of the eukaryotic plasma membrane due to chemical and mechanical challenges are frequent and detrimental and thus need to be repaired to maintain proper cell function and avoid cell death. However, the cellular mechanisms involved in wound resealing and restoration of homeostasis are diverse and contended. Here, it is shown that clathrin-mediated endocytosis is induced at later stages of plasma membrane wound repair following the actual resealing of the wound. This compensatory endocytosis occurs near the wound, predominantly at sites of previous early endosome exocytosis which is required in the initial stage of membrane resealing, suggesting a spatio-temporal co-ordination of exo- and endocytosis during wound repair. Using cytoskeletal alterations and modulations of membrane tension and membrane area, membrane tension is identified as a major regulator of the wounding-associated exo- and endocytic events that mediate efficient wound repair. Thus, membrane tension changes are a universal trigger for plasma membrane wound repair modulating the exocytosis of early endosomes required for resealing and subsequent clathrin-mediated endocytosis acting at later stages to restore cell homeostasis and function.