Temperature Conditions Research Articles

Experimental maturation of pine resin in sediment to investigate the formation of synthetic copal and amber

Experimentally simulating fossil resin formation would improve our understanding of copal/amber and could simulate the diagenesis of resin inclusions. Resin from living Pinus underwent sediment-encased maturation under various temperature and pressure conditions. Light microscopy suggests that matured resin dries, possibly hardens, and darkens into a brittle, yellow–orange–brown translucent mass with increased luster, exhibiting flow lines, birefringence, conchoidal fracturing, and air pockets typical of copal/amber. Leached components were observed in the sediment. Infrared spectroscopy suggests that matured resins have spectra consistent with those of fossil resins and may exhibit similar differences from fresh resin—possibly decreased relative intensity of C=O stretching at ~ 1700 cm−1. Results suggest desiccation and volatile/labile component loss, alongside potential polymerization/cross-linking of stable components into a macromolecule (although we discuss the challenge of ‘Class V ambers’). ‘Synthetic copal/amber’ is amenable to destructive analyses and would guide studies of fossil resin inclusions in informed, predictable, and targeted manners to limit loss of rare specimens. With novel experimental methods involving fresh resin and utilizing sediment porosity, our work expands upon insights from commercial autoclaving of natural subfossil copal/fossil amber used to alter their physical properties. Considering the broad success of sediment-encased maturation to simulate carbonaceous compression fossils and ancient resins, we predict that experimental taphonomy will elucidate the fossilization potential of diverse plant biomolecules and even plant secondary metabolites related to herbivory, flavor, and pharmacology.

Relationships Between Temperature and Precipitation Conditions and Tree- Ring Growth in Küre Mountains National Park in the Context of Climate Change

Relationships Between Temperature and Precipitation Conditions and Tree- Ring Growth in Küre Mountains National Park in the Context of Climate Change

How warming impacts the photosynthetic physiology of the bloom-forming cyanobacterium, Microcystis aeruginosa, under UV exposure.

Microcystis aeruginosa is a common cyanobacterium leading to algal blooms. Coupled effects of temperature increase and UV radiation increase will affect its photosynthesis performance, which may in turn will affect its proliferation and distribution, and change the environmental health of the water body. In this study, M. aeruginosa FACHB 469 was incubated at 25°C and 30°C and subjected to photosynthetically active radiation (PAR) and UV radiation (PAR + UVR) to monitor the relevant physiological responses. Exposure to both PAR and PAR + UVR resulted in a decline in PSII maximum quantum yield of M. aeruginosa, with UVR having more significant inhibitory effect. Meanwhile, UVR significantly increased the PSII photoinactivation rate constant (Kpi) and decreased the PSII repair rate constant (Krec), whereas the warming did not have a significant effect on it, and no significant interaction effect between warming and UVR was observed. Further analysis of the strategies of algal cells to cope with UVR at different temperatures revealed that at 25°C, algal cells mainly relied on the repair cycle of PSII, and reduced the content of phycocyanin to lower light energy capture, and increased superoxide dismutase (SOD) and catalase (CAT) activities to alleviate the damage of UVR; whereas under warming conditions, algal cells, while relying on PSII repair, mainly photoprotect by strengthening the NPQ mechanism, thus improving their tolerance to UVR. These findings suggest that the differential strategies employed by M. aeruginosa to cope with UVR under varying temperature conditions may influence the resilience of cyanobacterial blooms to environmental stressors in the future.

Global hotspots of butterfly diversity are threatened in a warming world.

Insects are in decline and threatened by climate change, yet lack of globally comprehensive information limits the understanding and management of this crisis. Here we uncover a strong concentration of butterfly diversity in rare and rapidly shrinking high-elevation climates. Integrating comprehensive phylogenetic and geographic range data for 12,119 species, we find that global centres of butterfly richness, range rarity and phylogenetic diversity are unusually concentrated in tropical and subtropical mountain systems. Two-thirds of the assessed species are primarily mountain dwelling and mountains hold 3.5 times more butterfly hotspots (top 5%) than lowlands. These hotspots only partially overlap with those of ants, terrestrial vertebrates and vascular plants (14-36%), while butterfly diversity is uniquely concentrated above 2,000 m elevation. We project that up to 64% of the temperature niche space of butterflies in tropical realms will erode by 2070, with the geographically restricted temperature conditions of mountains potentially turning these from refugia to traps for butterfly diversity. Our study identifies critical conservation priorities for butterflies and underscores the need for quantitative global assessments of at least select insect groups to help mitigate biodiversity loss in a rapidly warming world.

The Miscibility of Hydrogen and Water in Planetary Atmospheres and Interiors

Abstract Many planets in the solar system and across the Galaxy have hydrogen-rich atmospheres overlying more heavy element-rich interiors with which they interact for billions of years. Atmosphere–interior interactions are thus crucial to understanding the formation and evolution of these bodies. However, this understanding is still lacking in part because the relevant pressure–temperature conditions are extreme. We conduct molecular dynamics simulations based on density functional theory to investigate how hydrogen and water interact over a wide range of pressure and temperature, encompassing the interiors of Neptune-sized and smaller planets. We determine the critical curve at which a single homogeneous phase exsolves into two separate hydrogen-rich and water-rich phases, finding good agreement with existing experimental data. We find that the temperature along the critical curve increases with increasing pressure and shows the influence of a change in fluid structure from molecular to atomic near 30 GPa and 3000 K, which may impact magnetic field generation. The internal temperatures of many exoplanets, including TOI-270 d and K2-18 b, may lie entirely above the critical curve: the envelope is expected to consist of a single homogeneous hydrogen–water fluid, which is much less susceptible to atmospheric loss as compared with a pure hydrogen envelope. As planets cool, they cross the critical curve, leading to rainout of water-rich fluid and an increase in internal luminosity. Compositions of the resulting outer, hydrogen-rich and inner, water-rich envelopes depend on age and instellation and are governed by thermodynamics. Rainout of water may be occurring in Uranus and Neptune at present.

Enhanced Stability and Adsorption of Cross-Linked Magnetite Hydrogel Beads via Silica Impregnation

Hydrogel-based adsorbents have gained increasing recognition in recent years due to their promising potential for pollutant removal. However, conventional hydrogels often suffer from low mechanical strength over prolonged use. Therefore, this study explores the incorporation of silica extracted from bamboo culm (Dendrocalamus asper) to enhance the mechanical stability of hydrogel beads composed from carboxymethyl cellulose (CMC), chitosan (CS), and magnetite ferrofluid (Fe3O4), through cross-linking. We hypothesize that silica enhances the mechanical properties of magnetite hydrogel beads without compromising their adsorption capacity. The extracted silica was confirmed with FTIR and EDS analysis. The synthesized CMC-CS-Fe3O4-Si hydrogel beads were characterized using FTIR and SEM. Its stability was assessed through dry weight loss measurements, while its adsorption efficiency was evaluated using batch adsorption experiments. The silica-incorporated hydrogel exhibited enhanced mechanical and thermal stability under various pH and temperature conditions, without negatively affecting its adsorption performance, achieving maximum adsorption capacities of 53.00 mg/g for Cr (VI) and 85.06 mg/g for Cu (II). Desorption and regeneration studies confirmed the reusability of the hydrogel for more than four cycles. Overall, the interaction between the hydrogel and silica resulted in excellent adsorption performance, improved mechanical properties, and long-term reusability, making this a promising hydrogel adsorbent for wastewater remediation.

Optimization of freeze-drying process for anti-Chlamydia hyperimmune serum

Introduction. The distribution are a of Chlamydia infection in livestock and wild animals currently extends across almost all continents. At present, initial diagnosis, screening tests and certain stages of epizootiological investigations aimed at Chlamydia carrier detection are conducted in the Russian Federation using the “Antigen and Serum Kit for Serological Diagnosis of Chlamydiosis in Livestock”. It is important in theproduction of diagnostics to ensure stability of different test kit components during their storage and transportation. One way of addressing this issue is to stabilize diagnosticum components by freeze-drying.Objective. The study was aimed at optimization of freeze-drying process for specific anti-Chlamydia serum, the serum assessment for compliance with characteristics laid down in the technical specifications for the test kit control and testing of the serum for its stability.Materials and methods. The serum was prepared using blood from sheep immunized with emulsion vaccine based on Chlamydia psittaci AMK-16 strain. Prior tofreeze-drying, the hyperimmune sera were subjected to freezing to a temperature of minus 60 °С. The sera were freeze-dried using a Scientz 30F freeze-dryer (China). Two freeze-drying procedures with different temperature conditions and chamber pressures were applied. The resulting sera were tested for compliance with the technical specifications for thediagnostic test kit. The freeze-driedsera wereput intostorage for 24 months andtested with complement fixation test during this period.Results. Based on the test results, the freeze-drying procedure employing a lower pressure and the highest heating temperature was found to be the most effective for the specific sera. The serum tests for compliance with characteristics laid down in the technical specifications for the test kit showed that the serum quality met all relevant requirements. The stability test results demonstrated that the hyperimmune serum freeze-dried using the improved procedure remains specific during 24 months.Conclusion. The work performed allowed for optimization of freeze-drying process for specific anti-Chlamydia serum intended for the diagnostic test kit. The resulting serum is fully compliant with characteristics laid down in thetechnical specifications for the diagnosticum. It was established that the freeze-dried serum shelf life is at least two years, during this period the serum retains its activity and physico-chemical properties.

Probiotic characterization, safety assessment, and production performance of a novel probiotic strain of Bacillus subtilis SKB/2074 (MCC 0563) in broiler chickens

Probiotics are living microorganisms when administered in adequate amounts confer health benefits to the host. In the present study, a soil isolate was identified as Bacillus subtilis based on the 16S rRNA sequencing. In probiotic functional characterization (in vitro), B. subtilis SKB/2074 produced 10 different enzymes, was stable under simulated gastric conditions (pH 2.5/1–3 hr), bile salt (0.05–0.3% w/v), and temperature (40–90 °C) conditions. B. subtilis SKB/2074 cells were non-hemolytic, found susceptible to the 30 antibiotics, and showed antimicrobial activity against Escherichia coli, Salmonella typhimurium, and Clostridium perfringens. In in vivo studies, B. subtilis SKB/2074 demonstrated encouraging results to reverse E. coli and castor oil incited diarrhea in Wistar rats and Albino mice, respectively. Histopathological studies exhibited restoration of damaged mucosal epithelium cells and recovers veracity of goblet cells (colon). B. subtilis SKB/2074 exhibited immunomodulatory effects (increased immunoglobulins in blood and weight of spleen and thymus) and significant antioxidant activity (84.14%), reducing capacity and ascorbate auto-oxidation inhibition effect (95.13%). In poultry field studies, B. subtilis SKB/2074 significantly improved growth performance and lowered mortality rate in broiler chickens. Based on these preliminary scientific assessments B. subtilis SKB/2074 is likely to be used as potential probiotic and antidiarrheal agent in humans and animal healthcare.

VaMIEL1-Mediated Ubiquitination of VaMYB4a Orchestrates Cold Tolerance through Integrated Transcriptional and Oxidative Stress Pathways in Grapevine

Abstract Cold stress poses a significant threat to viticulture, particularly under the increasing pressures of climate change. In this study, we identified VaMIEL1, a RING-type E3 ubiquitin ligase from Vitis amurensis, as a negative regulator of cold tolerance. Under normal temperature conditions, VaMIEL1 facilitates the ubiquitination and subsequent proteasomal degradation of the cold-responsive transcription factor VaMYB4a, thereby attenuating its regulatory role in the CBF-COR signaling cascade. However, under cold stress, VaMIEL1 expression is downregulated, leading to the stabilization of VaMYB4a and the activation of CBF-COR signaling.Through a combination of biochemical assays and functional analysis in Arabidopsis thaliana and grapevine calli, we demonstrate that VaMIEL1 overexpression reduces cold tolerance, as evidenced by increased oxidative stress, excessive ROS accumulation, and downregulated expression of cold-responsive genes. Conversely, silencing of VaMIEL1 enhances cold tolerance by stabilizing VaMYB4a and boosting antioxidant defenses. These findings uncover a previously unrecognized regulatory mechanism by which VaMIEL1 modulates cold tolerance through transcriptional and oxidative stress pathways, offering potential targets for the development of climate-resilient grapevine cultivars and other crops.

Effect of heavy metal copper on the physiological characteristics of Ulva lactuca at different temperatures

Copper (Cu) is an essential element for macroalgae and has been extensively studied, but the interactive effects of temperature and Cu on these organisms remain less understood. In this study, we measured the photosynthetic characteristics of Ulva lactuca exposed to varying Cu concentrations and different temperatures (10 °C, 15 °C, and 20 °C). The results indicated that at the same temperature, as the concentration of Cu increased, the relative growth rate of U. lactuca showed a decreasing trend. Under three different temperatures, the photosynthetic rate and chlorophyll content of the algae significantly decreased with the increase in Cu concentration. Under the same Cu concentration conditions, as the temperature rises, the RGR of the algae gradually increases. In the case of low Cu concentration (LCu), the net photosynthetic rate at 15 °C and 20 °C increased by 103.72% and 104.97%, respectively, compared to the rate at 10 °C. Under high Cu concentration (HCu), the net photosynthetic rate at 15 °C and 20 °C increased by 192.18% and 245.67%, respectively, compared to that at 10 °C. The pigment content showed a similar trend. These results indicated that under the same temperature conditions, high concentrations of Cu inhibited the growth of algae, while under the same Cu treatment conditions, a suitable increase in temperature could alleviate the toxic effects of Cu on the algae.

Exploring the relationships between extraction conditions and anti‐browning functionality of Codium sp. aqueous extract

AbstractBACKGROUNDCodium tomentosum, an edible green seaweed, shows significant potential as a natural anti‐browning agent for fresh‐cut apples, which often suffer from oxidation‐induced browning that limits shelf‐life. This study aimed to explore the effects of extraction conditions for C. tomentosum to maximize its anti‐browning functionality, focusing on key extraction parameters and exploring the underlying inhibitory mechanisms.RESULTSUsing Box–Behnken design, C. tomentosum was extracted under varying conditions of time (0–180 min), temperature (20–90 °C), and pH (3–10), followed by response surface methodology to analyze the effects. Higher extraction yields were achieved with extended extraction times (over 120 min) and pH levels between 6 and 10. Anti‐browning effectiveness was primarily influenced by temperature, with optimal inhibition observed at temperatures above 60 °C. 1H‐n(i, ii)uclear magnetic resonance analysis further identified galactans as key components in the extracts, likely contributing to the anti‐browning effect through their barrier‐forming properties and potential interactions with browning‐related enzymes.CONCLUSIONThe C. tomentosum extracts, even when subjected to intensified extraction conditions, demonstrated effective browning inhibition in fresh‐cut apples, providing a promising, natural approach for shelf‐life extension. This work highlights the potential of marine‐based resources in food preservation and offers insights into optimizing extraction processes for food industry applications. © 2025 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

A Comprehensive Review on the Rapid Hydrate Formation for CO2 Capture: Characteristics, Mechanism, and Applications

ABSTRACTCO2, being a major greenhouse gas, is regarded as an important contributor to global warming and environmental problems. CO2 capture and separation are an efficient approach for reducing CO2 emissions in the atmosphere. A hydrate method of CO2 capture and separation provides a feasible solution to the emission reduction of CO2 in the atmosphere. However, the rapid formation of hydrate is crucial for CO2 capture and separation using the hydrate technique. As a consequence, this paper comprehensively reviewed the rapid formation characteristics and the kinetic law of CO2 hydrate, as well as deeply analyzed the influences of temperature and pressure conditions, gas–liquid ratios, additives, hydration reaction system, hydration reaction process, and other factors on its formation process. On this basis, the quantitative impact and regulatory mechanisms of different factors on the nucleation and growth process of CO2 hydrate were comprehensively analyzed. The influence mechanisms and kinetic laws of temperature, pressure, gas–liquid ratio selection, additive concentration, and type of reaction system on CO2 hydrate rapid formation were detailed. The regulatory and enhancement mechanisms of CO2 hydrate rapid formation under multiple factors were elucidated. The application of CO2 capture by the hydrate method and its challenges are summarized. In the end, the key problems and future directions of rapid CO2 capture and separation using the hydrate method were pointed out. The synergistic mechanism of rapid CO2 hydrate formation and the enhancement through multiple factors still need to be further investigated. Developing new reactor structures and optimizing the hydration reaction process are important in promoting the rapid formation of CO2 hydrate.

Asphalt emissions in paving production, transportation, placement and in-service temperature conditions

The paving industry significantly contributes to elevated air pollutant emissions. This study analyses asphalt emissions during asphalt mixing plant (AMP) production, transport and placement in a real context of paving services and after compaction and cooling under operational conditions, which remains an underexplored aspect in scientific literature. Particulate matter (PM) with chemical speciation of volatile organic compounds (VOCs) was collected in AMP by HiVol. Innovative passive sampling methods were used for NO2 in field measurements, while, in the laboratory, air quality sensors collected NO2 and VOCs. NO2 results suggest that transport is the critical paving service, at least five times higher than production. Asphalt pavements exposed to daily heat cycles (∼65°C) are a continuous source of air pollution emissions in urban areas, showing the same concentration magnitudes of paving steps. This study can contribute to emission inventories, aiding the comprehension and depiction of factors that impact urban air quality.

Biodiesel production as an alternative for energetic valorization of biomass from poultry wastewater through Chlorella vulgaris and Novozyme 435

AbstractThis experiment used valorized biomass of Chlorella vulgaris obtained by a culture in poultry wastewater to successfully produce biodiesel through enzymatic transesterification with 1% Novozyme 435 (Candida antarctica lipase B, CAL B). To increase % biodiesel conversion utilizing valorized biomass lipids, the study sought to determine the ideal temperature (35°C and 45°C) and alcohol:oil molar ratio (6:1 and 9:1) for the transesterification reaction (two‐factor experimental design with two quantitative levels). It was found that the temperature of 35°C and the molar ratio of 9:1 are the temperature and molar ratio conditions that improve the conversion to biodiesel, achieving a % biodiesel conversion of 82% in 8 h of reaction (p < 0.05). Fatty acid methyl esters (FAME) were characterized by gas chromatography demonstrating the production of biodiesel, the parameters acid number, density, cloud point, refractive index, and flash point complied with ASTM standards, the rheological behaviour of the biodiesel obtained was Newtonian (n = 1).

Nanofiber Aerogel with Rigidity-Flexibility Synergy.

In aerogel-based thermal insulation materials, the challenge of balancing mechanical properties (rigidity and flexibility) while enhancing thermal performance under extreme temperature and humidity conditions persists. This study introduces an innovative biomimetic aerogel design combining features of shell-like layered architecture and loofah porous microstructures. We developed polyimide/polyvinylidene fluoride (PI/PVDF) nanofiber aerogels with excellent thermal insulation and mechanical properties. The material can withstand compressive loads up to 1500 times its weight with axial rigidity, while maintaining radial flexibility under 80% strain, thereby achieving a harmonious balance between structural rigidity and flexibility. The inclusion of hydrophobic PVDF nanofibers ensures the material maintains low thermal conductivity and structural integrity, even under extreme humidity and temperature changes. This multifeature fusion biomimetic aerogel shows great potential for aerospace applications, such as spacecraft thermal protection systems, effectively shielding components from thermal and mechanical stress during re-entry and space missions.

Estimation of power output in thermoelectric generators (TEG) modules with mismatching thermal conditions

ABSTRACT In industrial applications, thermoelectric generators (TEGs) are commonly constructed using several p-n types of thermoelectric (TE) materials interconnected in series. The TEG module is placed over the heat source to extract the maximum power output from a hot surface, with the opposite face exposed to a lower temperature. However, achieving a homogeneous temperature distribution on the module’s hot side can be challenging due to many industrial heat sources’ variable and non-homogeneous nature. As a result, mismatched temperature conditions are often encountered, with some TE pairs experiencing a different T h temperature from others. This can significantly impact the overall performance of the TEG module, necessitating comprehensive modeling techniques to predict the behavior of these systems under the Dirichlet and Neumann boundary conditions. This work presents a mathematical model for evaluating thermoelectric generators that includes the effect of temperature-dependent TE properties and conditions of mismatch. The model also introduces a novel method for solving the strongly non-linear problem with low computational cost. The one-dimensional equations for temperature profile, electrical potential, and heat generation in the steady state are derived using radial basis functions and the homotopy method.

Plastic Responses of Iris pumila Functional and Mechanistic Leaf Traits to Experimental Warming

Phenotypic plasticity is an important adaptive strategy that enables plants to respond to environmental changes, particularly temperature fluctuations associated with global warming. In this study, the phenotypic plasticity of Iris pumila leaf traits in response to an elevated temperature (by 1 °C) was investigated under controlled experimental conditions. In particular, we investigated important functional and mechanistic leaf traits: specific leaf area (SLA), leaf dry matter content (LDMC), specific leaf water content (SLWC), stomatal density (SD), leaf thickness (LT), and chlorophyll content. The results revealed that an elevated temperature induced trait-specific plastic responses, with mechanistic traits exhibiting greater plasticity than functional traits, reflecting their role in short-term acclimation. SLA and SD increased at higher temperatures, promoting photosynthesis and gas exchange, while reductions in SLWC, LDMC, LT, and chlorophyll content suggest a trade-off in favor of growth and metabolic activity over structural investment. Notably, chlorophyll content exhibited the highest plasticity, emphasizing its crucial role in modulating photosynthetic efficiency under thermal stress. Correlation analyses revealed strong phenotypic integration between leaf traits, with distinct trait relationships emerging under different temperature conditions. These findings suggest that I. pumila employs both rapid physiological adjustments and longer-term structural strategies to cope with thermal stress, with mechanistic traits facilitating rapid adjustments and functional traits maintaining ecological stability.

Plant cold acclimation and its impact on sensitivity of carbohydrate metabolism

The ability to acclimate to changing environmental conditions is essential for the fitness and survival of plants. Not only are seasonal differences challenging for plants growing in different habitats but, facing climate change, the likelihood of encountering extreme weather events increases. Previous studies of acclimation processes of Arabidopsis thaliana to changes in temperature and light conditions have revealed a multigenic trait comprising and affecting multiple layers of molecular organization. Here, a combination of experimental and computational methods was applied to study the effects of changing light intensities during cold acclimation on the central carbohydrate metabolism of Arabidopsis thaliana leaf tissue. Mathematical modeling, simulation and sensitivity analysis suggested an important role of hexose phosphate balance for stabilization of photosynthetic CO2 fixation. Experimental validation revealed a profound effect of temperature on the sensitivity of carbohydrate metabolism.

Comparative Multi-Omics Analysis Reveals Key Pathways in Chlorophyll Metabolism and Stress Adaptation in Poplar Under Dual Stress

Poplar anthracnose, caused by Colletotrichum gloeosporioides, significantly threatens global poplar cultivation, with rising temperatures further intensifying environmental stress on trees. As autotrophic organisms, plants rely on photosynthesis for growth and stress responses, making this process particularly vulnerable under combined stressors, such as heat and pathogen infection. This study investigates the dual-stress response mechanisms of the resistant poplar species Populus × canadensis through integrated transcriptomic and metabolomic analyses. Results show that C. gloeosporioides inoculation at ambient temperature conditions activates multiple defense-related pathways, including MAPK signaling and ferroptosis. High temperatures amplify these responses, leading to extensive alterations in gene expression, particularly in pathways related to the cell cycle, photosynthesis, and phytohormone signaling. The chlorophyll content, a key marker of photosynthetic efficiency, is significantly reduced under high temperatures, with dual stress causing the most pronounced declines in chlorophyll a and b and total chlorophyll levels. These findings provide valuable insights into the molecular mechanisms underlying poplar resilience to anthracnose and heat stress, offering a foundation for breeding climate-resilient and pathogen-resistant tree cultivars.

Thermal Tolerance of Crassostrea (Magallana) ariakensis to Nuclear Plant Warm Water Discharges

Nuclear power plants utilize great quantities of seawater to cool down, resulting in substantial warm water discharges that may affect nearby fisheries and marine ecosystems. This study focused on Crassostrea (Magallana) ariakensis, a commercially farmed oyster species along the southern coast of China. To evaluate the thermal impacts of warm water discharges from nuclear power plants, indoor simulations replicated seasonal water temperature conditions near coastal facilities (26 °C in spring and autumn, 16 °C in winter, and 30 °C in summer). We conducted thermal tolerance static and dynamic experiments, along with a 51-day long-term experiment on suitable growth under different acclimation temperatures. The thermal effects of warm water discharges on C. ariakensis were systematically assessed through survival, growth, digestibility, and nutritional quality. The results showed that the discomfort temperature range of C. ariakensis was (48.6 ± 1.2)~(58.9 ± 3.0) °C, the critical thermal maxima (CTM) value range of C. ariakensis was (51.6 ± 1.4)~(61.2 ± 2.2) °C, and the incipient lethal temperature (ILT50) of C. ariakensis was 45.61 °C, 53.71 °C, and 55.90 °C, respectively; all these values increased gradually with the rise of acclimation temperature. After the 51-day long-term experiment on suitable growth, the temperature increase of 1 °C, 2 °C and 4 °C did not affect the soft tissue wet weight, condition index, moisture content, and fat content of C. ariakensis, but the amylase activity in digestive gland tissue decreased in different temperature experimental groups. The experimental results show that the influence of temperature rise on the growth and physiological metabolism of C. ariakensis is limited. However, based on the normal habitat temperature in summer, the long-term effects of temperature rise caused by warm water discharges need to be paid attention to.