Microtechnique Research Articles

Domains and methodological contexts of value studies

This paper examines a number of domains and methodological contexts within which social values are investigated. The epistemic and methodological characteristics of these two types of areas of investigation—the former differentiated historically, and the latter chosen deliberately—are analysed. The analysis is done within an explicit conception of methodology—and of social values. The methodological contexts distinguished are: populations, situations of interaction, bounded collectivities, institutional structures and discourses. It is argued that life-worlds are natural and everyday life-and-living spaces and not exceptional or distinct methodological contexts. Methodological aspects examined include: conceptualisation, procedures and techniques of empirical substantiation, theorising, micro and macro investigations, and methodological equity.

Semiautomatic microtechnique for serial dilution-antibiotic sensitivity testing in the clinical laboratory.

Abstract A semiautomatic microtechnique diluter which has been adapted for serial dilution-antibiotic sensitivities and antibacterial assay of biological fluids is described. These tests can be performed rapidly, accurately, and conveniently. Antibiotic combination tests to demonstrate synergism, antagonism, or additive effects are easily done.

Plant Microtechnique: Some Principles and New Methods

Plant Microtechnique: Some Principles and New Methods

Useful techniques for micro and macro sublimation

Useful techniques for micro and macro sublimation

Essentials of Practical Microtechnique

Essentials of Practical Microtechnique

Microtechnique for the Infrared Study of Solids. Diamonds and Sapphires as Cell Materials

Microtechnique for the Infrared Study of Solids. Diamonds and Sapphires as Cell Materials

A Scintific Analysis of Fixation and Dyeing

Principles of Biological Microtechnique A Study of Fixation and Dyeing. By Dr. John R. Baker. Pp. 357. (London: Methuen and Co., Ltd.; New York: John Wiley and Sons, Inc., 1958.) 45s. net.

Microchemistry and ultramicrochemistry; history and development

Since the end of the last century new techniques have been developed to make it possible to perform chemical experiments on a smaller and smaller scale. The historical development ofgravimetry at a macro-, micro- and ultramicro-level, is represented in a cologarithmic diagram, correlating the sensitivity of the balance, the amount of compound to be evaluated and the weight of sample normally taken. Reference is made to the techniques of micro- and ultramicro-gravimetry used in the laboratory ofB. B. Cunningham at the University of California (Berkeley), and attention is directed to exercises made by graduate students in this laboratory. Titrimetry, with amounts of the microliter, is easy to make, for instance by means of the pipettes and burettes designed byP. Kirk. Ultramicro Kjeldahl determinations, for instance, are very useful for biological purposes; they can be effected by the diffusion technique ofConway, without transfer of solution. On the other hand, for the manipulation of radioactive compounds, operations in “gloved boxes” assure a satisfactory protection at a level lower than 100 millicuries. At a ultramicro level,spectrophotometry can be used with success and is a very promising method today. The sensitivity in the detection and evaluation of traces by means of different analytical procedures is compared graphically. A review is given of some analytical, preparative and physico-chemical methods used in micro- and ultramicro-chemistry.

Quick microtechniques for the identification of cultures; indole production.

Abstract A quick microtechnique for the determination of the abilities of micro-organisms to produce indole has been described. Heavy inoculations are made into 1 ml. quantities of medium in 10 by 75 mm. tubes that have been preheated to 37 °C. Either tryptone broth or a synthetic medium containing tryptophane has been found to be satisfactory. The tubes are then incubated at 37 °C., preferably in a water bath. Indole may be detected in the tubes upon the addition of 4-drop quantities of a fresh Kovacs solution which has been made with isoamyl alcohol as the solvent. The period of incubation that is necessary has been found to vary from six minutes to two hours, depending upon the strain of organism, the age and size of inoculum, and the method of incubation.

Studies on streptomycin.

Abstract Two methods, the micro and microfilm techniques for the quantitative determination of streptomycin and other antibiotics in body fluids are described in detail. Using these methods, 1.5 and 1.0 c.c., respectively, of body fluid are necessary for a standard curve and 0.06 c.c. for a level determination in quintuplet. Venous puncture is necessary only for obtaining blood for a standard curve. A drop of blood obtained from a finger or other convenient extremity is used for blood level determinations.

Some Suggestions in Microtechnique Particularly Useful in Microentomology and Parasitology

Some Suggestions in Microtechnique Particularly Useful in Microentomology and Parasitology

Micro-Technique: Suggestions for Methods and Apparatus

Micro-Technique: Suggestions for Methods and Apparatus