Myocardial ischemia/reperfusion (I/R) injury is a serious complication during the recanalization treatment of myocardial infarction (MI), which seriously affects the prognosis of patients. As a renowned traditional Chinese medicinal formulation, Si-Miao-Yong-An decoction (SMYA) has therapeutic effects on myocardial I/R injury, although its underlying mechanisms, particularly concerning regulated cell death pathways, are not fully understood. This study aimed to explore the therapeutic effect and possible mechanism of SMYA in myocardial I/R injury. C57BL/6J mice were divided into sham, model, and SMYA-treated groups (low, medium and high-dose). In this study, we identified 13 main components of SMYA using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Moreover, SMYA improved cardiac dysfunction caused by myocardial I/R injury, decreased the levels of myocardial injury markers, reduced the myocardial infarct size, and alleviated pathological changes in the myocardium. In addition, transcriptomic analysis predicted that the p53 signaling pathway may be a potential target for its function. To this end, the result of western blotting revealed that SMYA inhibited myocardial cell necroptosis by regulating the p53 and RIPK1/RIPK3/MLKL pathways. Overall, SMYA suppressed necroptotic processes induced by myocardial I/R injury, likely mediated by the modulation of the p53 and RIPK1/RIPK3/MLKL pathways. Therefore, SMYA may be a potential therapeutic agent for treating myocardial I/R injury.

Moutan Cortex (MC), the dried root bark of Paeonia suffruticosa, is used in traditional Chinese and Korean medicine to treat enteritis for its anti-inflammatory properties. This study compared the pharmacokinetic (PK) profiles of paeonol and paeoniflorin in normal and dinitrobenzene sulfonic acid (DNBS)-induced colitis rats, and to determine how repeated low-dose MC [MC(L), 0.5 g/kg] or high-dose MC [MC(H), 2.5 g/kg] alters PK and disease severity. Using ultra-performance liquid chromatography–tandem mass spectrometry, we found that DNBS modestly increased paeonol AUClast (NC: 247.8 ± 63.7 vs DNBS: 337.0 ± 120.8 hr*ng/mL) and decreased paeoniflorin (NC: 474.1 ± 11.7 vs DNBS: 463.7 ± 106.8 hr*ng/mL) compared to controls (ns). After repeated dosing, the maximum plasma concentration (Cmax) of paeonol was higher in the MC(H) than that in the MC(L) group (MC(L): 63.81 ± 29.74 vs MC(H): 4221.5 ± 1579.2 ng/mL, p < 0.05). Paeoniflorin Cmax in the MC(H) group was also higher than MC(L) group (MC(L): 60.5 ± 15.3 vs MC(H): 164.7 ± 74.7 ng/mL, p < 0.05). Repeated MC(H) treatments improved body weight loss and disease activity index. Western blots indicated that the expression of intestinal epithelial integrity-related proteins in the MC(H) group was comparable to that in the control. Inflammation did not influence paeonol and paeoniflorin PK significantly, whereas MC(H) group markedly increased systemic exposure, especially of paeonol, and demonstrated symptom relief. Appropriate dose adjustments are necessary to ensure safe and effective therapy because PK changes can lead to increased systemic exposure and affect treatment outcomes.

Comparing persistent (polycyclic aromatic hydrocarbons and polychlorinated biphenyls) and emerging nano(micro)plastics (NMPs) pollutant body-burdens in oysters and fish from Matagorda Bay (Texas, USA).

Dietary exposure to hexafluoropropylene oxide dimer acid (HFPO-DA ammonium salt, GenX) alters hepatic sphingomyelin and phosphatidylcholine metabolism in Nile Tilapia (Oreochromis niloticus).

LC-MS/MS method development and validation for novel targeted anticancer therapies adagrasib, capmatinib, ensartinib, entrectinib, larotrectinib, lorlatinib, pralsetinib, selpercatinib and sotorasib.

Comprehensive assessments of pharmacokinetics and immunogenicity of farletuzumab ecteribulin, a novel antibody-drug conjugate against tumors expressing folate receptor α, in monkeys.

New salty extraction of polar analytes technique and oil-based cleanup (SExPA-Oc) for the determination of nereistoxins in vegetable samples with HILIC-MS/MS.

Metabolite identification remains a bottleneck in untargeted liquid chromatography-tandem mass spectrometry (LC-MS) metabolomics studies, particularly when the underlying metabolite is absent in the tandem mass spectrometry (MS/MS) databases. A new approach, formula subset analysis (FSA), was developed to effectively prescreen and rank the chemical formula candidates for an MS/MS spectrum. This approach first computes mother-daughter relationships (MDRs) among possible formulas of fragments and the precursor under a given mass tolerance and then determines the characteristic fragments (CFs) that only present one MDR with the precursor and other fragments. Subsequently, the precursor formula candidates are ranked by the scores derived from the number of MDRs. A numerical study using eight large datasets totaling 30,690 MS/MS spectra from 6792 metabolites consisting of C, H, O, N, S, and P showed that FSA ranked the correct chemical formula as the top-1 candidate for a metabolite in 85.28% of the cases and in the top-5 candidates in 97.35% of the cases. The average processing time for each spectrum was 0.024s. Moreover, FSA does not require training data, not rely on MS/MS databases, can be applied to a wide mass range, and can be quickly expanded with more chemical elements and formulas to identify different chemical species. FSA has not utilized structural information yet and therefore its accuracy may not be competitive with some of the state-of-the-art identification tools. However, its advantages in speed, expandability, and applicability, make it suitable for prescreening candidates in untargeted LC-MS metabolomics studies.

Batchwise data analysis with inter-batch feature alignment in large scale platelet lipidomics study using UHPLC-ESI-QTOF-MS/MS by data-independent SWATH acquisition.

An improved pretreatment method for efficient detection of antibiotic residues in aquatic products by LC-MS/MS.

First report of borbotoxin occurrence in Brazilian Prorocentrum borbonicum isolates: LC-MS/MS detection and preliminary structural insights.

Assessing the effect of a non-ionic surfactant on surface degradation of the pesticide Acetamiprid on live tea plants.

Association between polygenic risk scores and vitamin D status among Chinese healthy infants and toddlers: The role of dietary intake and supplementation practice.

Determining carbon-carbon double bond position of unsaturated glycerophospholipids in human plasma NIST® SRM® 1950 by electron impact excitation of ions from organics-tandem mass spectrometry (EIEIO-MS/MS).

Unraveling the plasma dynamics, oxidative metabolism and excretion behavior of M-PEG6-OH by UPLC-MS/MS.

Estimation and risk assessment of pesticides and their toxicologically relevant metabolites in cereals using modified QuEChERS method and analysis by Gas Chromatography tandem Mass Spectrometry (GC-MS/MS)

Shining a light on hidden PFAS: Development of a UV-reducible organic fluorine assay for the rapid screening of PFAS in AFFF, water, soils, and commercial products.

Origin-dependent metabolic variations: How Atractylodes macrocephalae Rhizoma extract's chemical diversity leads to stage-specific changes in simulated digestion.

Enhancement and mechanism of in-cluster two-component regulatory factors on granaticin production in Streptomyces vilmorinianum YP1.

Dark tea extract extends Caenorhabditis elegans lifespan by enhancing antioxidant and ferroptosis resistance.