You have accessJournal of UrologyKidney Cancer: Basic Research II1 Apr 2014MP29-16 FABP7 AS A POTENTIAL TARGET AND MARKER IN CLEAR CELL RENAL CELL CARCINOMA Kazuhiro Nagao, Nachi Shinohara, Frank Smit, Sander Jannink, Mirjam de Weijert, Keita Kobayashi, Yoshihisa Kawai, Hiroaki Matsumoto, Takahiko Hara, Hideyasu Matsuyama, Yuji Owada, Peter Mulders, and Egbert Oosterwijk Kazuhiro NagaoKazuhiro Nagao More articles by this author , Nachi ShinoharaNachi Shinohara More articles by this author , Frank SmitFrank Smit More articles by this author , Sander JanninkSander Jannink More articles by this author , Mirjam de WeijertMirjam de Weijert More articles by this author , Keita KobayashiKeita Kobayashi More articles by this author , Yoshihisa KawaiYoshihisa Kawai More articles by this author , Hiroaki MatsumotoHiroaki Matsumoto More articles by this author , Takahiko HaraTakahiko Hara More articles by this author , Hideyasu MatsuyamaHideyasu Matsuyama More articles by this author , Yuji OwadaYuji Owada More articles by this author , Peter MuldersPeter Mulders More articles by this author , and Egbert OosterwijkEgbert Oosterwijk More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.758AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To identify potential targets in clear cell renal cell carcinoma (ccRCC), we performed a transcriptome analysis of ccRCC and normal kidney specimens. The transcriptome analysis revealed numerous highly overexpressed genes related to hypoxia, pH regulation and metabolisms in ccRCC compared to normal kidney specimens. FABP7 (Fatty Acid Binding Protein 7) is supposed to be involved in fatty acid synthesis in cancer cells and was highly overexpressed in ccRCC specimens compared to normal kidney specimens. We confirmed the significance of FABP7 as a potential target and marker in ccRCC. METHODS We performed a gene expression analysis against the total RNA extracted from 60 ccRCC and 20 normal kidney specimens on the human exon 1.0 ST array (Affimetrix). The expression profiles were analysed by unsupervised hierarchical average linkage clustering algorithm. Among the overexpressed genes, FABP7 had the highest mean difference between ccRCC and normal kidney in the profile. The expression levels were further studied by immunohistochemical analysis in 19 advanced ccRCC cases treated by molecular targeted drugs. The mRNA expression levels were evaluated in 8 ccRCC cell lines and the effect of gene knockdown was studied in a highly expressed cell line (A498). RESULTS Among the overexpressed genes, FABP7 had the highest mean expression level in mRNA, while the expression levels varied depending on the ccRCC cases. Our immunohistochemical analysis for the ccRCC specimens demonstrated significant correlation between FABP7 expression and distant metastasis (p<0.05). Based on proliferation assay (MTT assay), functional suppression of FABP7 resulted in significant growth inhibition of A498 cells (p<0.05). CONCLUSIONS Functional suppression for FABP7 downregulated the cell viability in a ccRCC cell line (A498). FABP7 expression level was significantly correlated with distant metastasis. FABP7 could be a potential therapeutic target and diagnostic marker to detect metastasis of ccRCC. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e311 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Kazuhiro Nagao More articles by this author Nachi Shinohara More articles by this author Frank Smit More articles by this author Sander Jannink More articles by this author Mirjam de Weijert More articles by this author Keita Kobayashi More articles by this author Yoshihisa Kawai More articles by this author Hiroaki Matsumoto More articles by this author Takahiko Hara More articles by this author Hideyasu Matsuyama More articles by this author Yuji Owada More articles by this author Peter Mulders More articles by this author Egbert Oosterwijk More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...