Surface Pre-reacted Glass-ionomer Research Articles

Effect of a Toothpaste Containing Surface Pre-Reacted Glass-Ionomer Filler on the Remineralization of Artificial Carious Enamel Lesions in situ

This study evaluated the remineralizing effect of a toothpaste containing surface pre-reacted glass-ionomer (S-PRG) filler on demineralized enamel in situ. For this, 180 bovine enamel samples were demineralized by using a microcosm biofilm model for 3 days. Thereafter, the samples were randomly signed to 15 healthy volunteers and to 3 cross-over in situ phases corresponding to the following treatments: (1) toothpaste containing 1,500 ppm F as NaMFP (positive control, Colgate®Cavity Protection), (2) toothpaste containing 5% S-PRG filler (Shofu®), and (3) placebo toothpaste (negative control prepared by Shofu®). Four demineralized enamel blocks were fixed into each palatal appliance per phase. The volunteers wore the appliances for 5 days and were trained to brush their teeth 2 times for 2 min a day, while one drop of the toothpaste’s slurry (1:3) was dripped on each sample for the same period. The surface hardness and TMR analyses were done and analyzed by ANOVA/Tukey and t test (p < 0.05). S-PRG filler and Colgate® toothpastes were equally able to improve 2–2.5× enamel remineralization by the analysis of % surface hardness recovery. However, S-PRG toothpaste was the only one able to significantly improve ΔΔZ (the integrated mineral loss recovery: 1,489 ± 503 %vol.μm) compared to placebo (1,050 ± 467 %vol.μm), while Colgate® did not differ from placebo. No differences were seen between the groups with respect to ΔLD. S-PRG filler and Colgate® toothpastes show similar potential to remineralize the lesion surface. However, S-PRG toothpaste is better to recover mineral loss at the subsurface area.

Effect of surface prereacted glass ionomer nanofillers on fluoride release, flexural strength, and surface characteristics of polymethylmethacrylate resin.

Dentures should have proper fluoride release and physical properties. We evaluated how surface prereacted glass ionomer (S-PRG) nanofillers influenced fluoride release, flexural strength, and surface characteristics of polymethylmethacrylate (PMMA) resin. PMMA resin disc (n=14) and rectangular (n=5) specimens containing 0, 20 wt% microparticles, and 20 wt% nanoparticles of S-PRG were prepared. Six-disc specimens were examined for surface roughness; eight-disc specimens were immersed in 5ml of deionized water for 24 h before analyzing the fluoride levels on days 1-3, 12, and 15. They were recharged with 1000 ppm fluoride solution for 24 h and stored in deionized water for five cycles. Fluoride release was examined. The flexural strength of the rectangular specimens was determined using a three-point bending test. Data were analyzed by two-way repeated-measures ANOVA. S-PRG nanofiller had the highest fluoride exchange rate and did not significantly change the surface roughness compared with the microparticle and control groups; however, the nanofillers agglomerated and reduced the flexural strength to below 65 MPa. Incorporating 20 wt% nanofillers into resin enhanced the fluoride exchange property greater than microfillers at the same content, but diminished the mechanical properties of the resin. Incorporating 20 wt% S-PRG nanofillers in resin denture base can improve the fluoride releasing property without affecting the surface roughness.

Surface pre-reacted glass-ionomer eluate protects gingival epithelium from penetration by lipopolysaccharides and peptidoglycans via transcription factor EB pathway.

Surface pre-reacted glass-ionomer (S-PRG) filler, produced by PRG technology for use with various dental materials, is bioactive and known to release ions from a glass-ionomer phase. We previously reported that coxsackievirus and adenovirus receptor (CXADR), a tight junction associated protein, was located in the epithelial barrier of gingival epithelium. In the present study, the tissue protective effects of an S-PRG eluate prepared with S-PRG filler were investigated using a three-dimensional human gingival epithelial tissue model. The results showed that the S-PRG eluate specifically induced CXADR expression at the transcriptional level of messenger RNA as well as the protein level, and also nuclear translocation of transcription factor EB (TFEB) in gingival epithelial cells. Furthermore, shigyakusan, a TFEB inhibitor, canceled induction of the CXADR protein by the S-PRG eluate. Additionally, gingival epithelial permeation by 40-kDa dextran, lipopolysaccharide, and peptidoglycan in the 3D-tissue models was prevented by the eluate, with those effects abrogated by knockdown of CXADR. These findings suggest that S-PRG eluate increases CXADR expression via the TFEB pathway, thus inhibiting penetration of bacterial virulence factors into subepithelial tissues.

A Scanning Electron Microscope Evaluation of the Efficacy of Different Fluoride-releasing Dental Restorative Materials to Prevent Enamel Demineralization: An In Vitro Study

Aim of the present research was to investigate the effectiveness of various fluoride-releasing dental restorative agents in preventing demineralization of enamel. Eighty human mandibular permanent molar teeth constituted the study group. All samples were subjected to storage in thymol, after which they were taken out to prepare alike proximal box in each. Inductions of artificial enamel surface lesions were done by placing the teeth in demineralizing solution for 96 hours. Subsequently, all 80 molars were randomly assigned to any of the four groups (i.e., 20 in every individual group) according to the restoration as group A: giomer (composite resin containing surface pre-reacted glass-ionomer fillers), group B: compomer (polyacid-modified composite resin), group C: resin-modified glass-ionomer cement (RMGIC), group D: fluoride-releasing composite. After this, the pH cycling was performed, and the samples were subjected to examination beneath scanning electron microscope (SEM). Higher mean areas of remineralization were noted when RMGIC (96.34 ± 0.06) was used followed by the compomer (109.52 ± 0.17), giomer (118.39 ± 0.82), and the fluoride-releasing composite group (129.27 ± 0.31) in that order. A statistically significant difference was seen amid the investigational groups that utilized different restorative agents (p <0.001). A pairwise evaluation that was performed revealed that except for the giomer group and the compomer group, a statistically significant difference (p <0.001) was found among the experimental groups. This research infers that the RMGIC-treated samples exhibited significantly superior performance in preventing enamel demineralization in comparison to compomer, giomer as well as fluoride-releasing composites. One among the highly frequently employed anticariogenic materials is fluorides. Owing to this characteristic, they are integrated into numerous restorative substances. Nevertheless, the quantity and speed of fluoride release differ in different agents, which translates to the efficacy of the restorative agent in avoiding demineralization about the restoration.

S-PRG-based composites erosive wear resistance and the effect on surrounding enamel

This study evaluated Surface Pre-Reacted Glass-ionomer (S-PRG)-based-composites’ surface resistance against erosive wear and their protective effect on surrounding enamel. Bovine enamel was randomized into 12 groups (n = 10/group) [erosion (e) or erosion + abrasion (a)]: nanohybrid-S-PRG-based composite (SPRGe/SPRGa), nanohybrid-S-PRG-based bulk-fill (SPRGBFe/SPRGBFa), nanoparticle-composite (RCe/RCa), nanohybrid-bulk-fill (BFe/BFa), Glass Hybrid Restorative System (GHRSe/GHRSa), and resin-modified glass-ionomer-cement (RMGICe/RMGICa). Cavities were prepared and restored. Initial profile assessment was performed on material and on adjacent enamel at distances of 100, 200, 300, 600, and 700 μm from margin. Specimens were immersed in citric acid (2 min; 6×/day for 5 days) for erosion. Erosion + abrasion groups were brushed for 1 min after erosion. Final profile assessment was performed. Two-way ANOVA and Tukey-test showed: for erosion, the GHRSe and RMGICe presented greater material wear compared to the other groups (p = 0.001); up to 300 μm away from restoration, GHRSe and SPRGBFe were able to prevent enamel loss compared to RMGICe and other composite groups (p = 0.001). For erosion + abrasion, none of the materials exhibited a significant protective effect and S-PRG-based groups showed lower wear than RMGICa and GHRSa, and higher wear than composites (p = 0.001). S-PRG-based-composites can diminish surrounding enamel loss only against erosion alone, similarly to GIC, with advantage of being a more resistant material.

Sensitivity Treatments for Teeth with Molar Incisor Hypomineralization: Protocol for a Randomized Controlled Trial.

BackgroundThe sensitivity of teeth with molar incisor hypomineralization (MIH) can affect children’s quality of life and is a challenging problem for dentists. Remineralizing agents such as sodium fluoride varnish seem to reduce the sensitivity of teeth with MIH, but long-term clinical trials with large samples are still needed for more evidence about its effectiveness as a desensitizing agent before its clinical recommendation.ObjectiveThis randomized clinical trial aims to compare three treatment interventions for teeth with MIH and hypersensitivity.MethodsA total of 60 children aged 6-10 years presenting with at least one first permanent molar with sensitivity and no loss of enamel will be randomly assigned to three groups: the control group (sodium fluoride varnish; Duraphat, Colgate); experimental group I (4% titanium tetrafluoride varnish); and experimental group II (a coating resin containing surface prereacted glass-ionomer filler; PRG Barrier Coat, Shofu). The sodium fluoride varnish and 4% titanium tetrafluoride varnish will be applied once per week for 4 consecutive weeks and the PRG Barrier Coat resin will be applied in the first session and the application will be simulated the following 3 weeks to guarantee the blinding of the study. The primary outcome will be sensitivity level measured at different moments (before each material application, immediately after application or simulation, and 1, 2, 4, and 6 months after the last application/simulation) by one examiner using the Wong-Baker FACES Pain Rating Scale, the Schiff Cold Air Sensitivity Scale, and the FLACC (Face, Legs, Activity, Cry, Consolability) scale. As secondary outcomes, parental satisfaction and child self-reported discomfort after the treatment will be measured with a questionnaire prepared by the researcher. The data will undergo statistical analysis and the significance level will be set at 5%.ResultsThe project was funded in 2018, and enrollment was completed in November 2019. The recruitment of participants is currently underway and the first results are expected to be submitted for publication in 2022.ConclusionsIf found effective in reducing the patient’s sensitivity long term, these agents can be considered as a treatment choice, and the findings will contribute to the development of a treatment protocol for teeth with sensitivity due to MIH.Trial RegistrationBrazilian Registry of Clinical Trials Universal Trial Number U1111-1237-6720; https://tinyurl.com/mr4x82k9International Registered Report Identifier (IRRID)DERR1-10.2196/27843

Surface Pre-reacted Glass-ionomer (S-PRG) Filler Eluate Suppresses MMP-1 Secretion by TNF-α-stimulated Human Gingival Fibroblasts

Surface Pre-reacted Glass-ionomer (S-PRG) Filler Eluate Suppresses MMP-1 Secretion by TNF-α-stimulated Human Gingival Fibroblasts

In vivo Assessment of Tissue Compatibility of Root Canal Sealer Containing Surface Pre-reacted Glass-ionomer Fillers

<i>In vivo</i> Assessment of Tissue Compatibility of Root Canal Sealer Containing Surface Pre-reacted Glass-ionomer Fillers

Physical properties and wear behavior of CAD/CAM resin composite blocks containing S-PRG filler for restoring primary molar teeth

ObjectivesThis study aimed to develop computer-aided design/computer-aided manufacturing (CAD/CAM) resin composite blocks (RCBs) containing surface pre-reacted glass-ionomer (S-PRG) filler for primary molar teeth and evaluate their physical properties and wear resistance. MethodsExperimental CAD/CAM RCBs containing S-PRG filler for primary molar teeth (EB), a commercial CAD/CAM RCB (HC), two resin composites for primary teeth (BKP and BKZ) and one for permanent teeth (BⅡ) were used. Hardness tests, three-point bending tests, fracture toughness tests, and water absorption tests were conducted. Wear tests were conducted for these materials and stainless steel crowns (SSCs). ResultsThe Vickers hardness of EB was lower than that of HC (p < 0.05), and there was no significant difference among BKZ, BKP, and BⅡ (p > 0.05). After 1 week of water immersion, EB and HC showed greater flexural strength than the other materials (p < 0.05). EB showed greater fracture toughness than the other materials (p < 0.05). The water absorption of EB was lower than that of HC, BKZ, and BKP (p < 0.05), and greater than that of BⅡ (p < 0.05). Antagonist wear was significantly smaller in EB than in HC and BⅡ (p < 0.05), and significantly greater than in BKZ (p < 0.05). Antagonist wear could not be measured in SSC because of excessive wear that was out of range of the surface roughness tester. SignificanceThe CAD/CAM RCBs containing S-PRG filler for primary molar teeth developed in this study demonstrated adequate physical properties and wear performance, suggesting that they are suitable for restoration of primary molar teeth and could function in place of SSCs.

Inhibitory effect of a gel paste containing surface pre-reacted glass-ionomer (S-PRG) filler on the cariogenicity of Streptococcus mutans

Surface pre-reacted glass-ionomer (S-PRG) filler is a bioactive functional glass that releases six different ions. Although several dental materials containing S-PRG filler have been developed, few self-care products containing S-PRG filler have been reported. We investigated the inhibitory effects of PRG gel paste containing S-PRG filler on Streptococcus mutans, a major pathogen of dental caries. PRG gel paste inhibited bacterial growth of S. mutans in a concentration-dependent manner, and all S. mutans were killed in the presence of ≥ 1% PRG gel paste. Additionally, it was difficult for S. mutans to synthesize insoluble glucan from sucrose in the presence of 0.1% PRG gel paste. A biofilm formation model was prepared in which slices of bovine enamel were infected with S. mutans after treatment with or without PRG gel paste. Biofilm formation was inhibited significantly more on the enamel treated with PRG gel paste than on enamel without PRG gel paste (P < 0.001). The inhibitory effects on bacterial growth and biofilm formation were more prominent with PRG gel paste than with S-PRG-free gel paste, suggesting that PRG gel paste may be effective as a self-care product to prevent dental caries induced by S. mutans.

Novel antimicrobial denture adhesive containing S-PRG filler.

The antimicrobial effects of denture adhesives containing novel surface pre-reacted glass-ionomer (S-PRG) fillers were assessed. We prepared denture adhesives containing S-PRG (particle sizes: 1 and 3 µm; quantities: 5, 7.5, and 10 wt%). We evaluated acid buffering capacity, ion release, and antimicrobial effects of denture adhesives with and without S-PRG. Significantly higher pH changes were observed in 1 µm S-PRG adhesives than in 3 µm S-PRG adhesives. Adhesives containing 7.5 and 10 wt% S-PRG exhibited significantly higher ion release than adhesives with 5 wt% S-PRG. The 1µm-10wt% S-PRG denture adhesive exhibited significantly lower colony-forming units on the denture adhesive contact surface than in the control group; additionally, it exhibited excellent acid buffering capacity, ion release properties, and antimicrobial effect against C. albicans, C. glabrata, S. mutans, and A. naeslundii. Longer contact periods resulted in significantly lower adhesion of Candida albicans to the denture base resin treated with denture adhesive.

Effects of multi-components released from S-PRG filler on the activities of human dental pulp-derived stem cells.

Numerous studies have shown that the sustained release of ions from dental restorative materials have acid buffering capacity, prevents tooth enamel demineralization, and inhibits bacterial adhesion. Herein, the release behavior and bioresponsiveness of ions released from surface pre-reacted glass-ionomer (S-PRG) fillers were investigated in different types of media based on human dental pulp-derived stem cell (hDPSC) responses. The hDPSCs were cultured for 1-7 days in S-PRG eluates diluted with varying amounts of cell culture media. S-PRG released several types of ions, such as F-, Sr2+, Na+, Al3+, BO33-, and SiO32-. The balance of eluted ions differed depending on the dilution and solvent, which in turn affected the cytotoxicity, cell morphology, cell proliferation, and alkane phosphatase activity of hDPSCs, among other properties. The results suggest that tailored S-PRG filler eluates could be designed and prepared for application in dental practice.

Prevention of acidic attack on tooth enamel surfaces using polishing paste containing ion-releasing filler.

The purpose of this study was to investigate the ability of polishing paste containing surface pre-reacted glass-ionomer (S-PRG) filler to prevent acidic attack on tooth enamel surfaces. Resin composites were filled in the standardized cavities and finished with silicon carbide paper. These specimens were divided into three groups: the unpolished "control" group, the "PRG" group polished with S-PRG paste, and the "DDP" group polished with diamond-containing polishing paste. Following polishing, the specimens were immersed in a lactic acid buffer solution for 28 days. Optical coherence tomography (OCT) signals were measured to obtain the signal intensity and width at 1/e2 at selected locations on the enamel surface adjacent to the restoration. Although signal intensity significantly increased in all groups, widths at 1/e2 did not change significantly in the PRG group. For both the control and DDP groups, signal intensity and width at 1/e2 increased and decreased over time, respectively.

Clinical Evaluation of Glass-Lonomer Cement and Giomer for the Management of Cervical Caries

Background: Giomer is a unique class of restorative material has been introduced as the true hybridization of Glass Ionomer (GI) and composite resin and has the distinguishing feature of a stable surface pre-reacted glass ionomer. Glass Ionomer Cements (GIC) are also unique restorative materials with many uses in clinical practice and provide for caries-protective fluoride releasing at the margins of restorations, as well as their ability to have the fluoride within their chemical matrix recharged by outside exposure to other fluoride-containing materials. The present study aim at clinically evaluate the comparison between the glass Ionomer cement and Giomer for the management of cervical caries.&#x0D; Materials and methods: The study was a cross sectional comparative study which was carried out from 01 December 2018 to 31 November 2019 in the Department of Conservative Dentistry and Endodontics, Bangladesh Dental College, Dhanmondi, Dhaka. Total number of cases was 80. The patients were divided into two groups, half of the patient (Group-1) was treated with Glass Ionomer cement and half of the patient (Group-2) was treated with Giomer restoration. The post-operative sensitivity discoloration, dislodgement of filling material and secondary caries formation were recorded. All the patients were assigned and the data were analyzed statistically by SPSS version 21. p-value &lt; 0.05 was taken as significant.&#x0D; Results: The study reveals 60% of the study population was within 41 to 50 years of age group (Group-1) and 62.5% were 51 to 60 years (Group-2) 45% in (Group-1) and 10% in (Group-2) had history of post filling sensitivity, 12% in (Group-1) and 5% in (Group-2) had discoloration after restoration, 2.5% had history of dislodgement of the filling in (Group-1), no history of dislodgement of the filling in (Group-2), 2.5% had history of secondary caries formation in (Group-1) and no history of Secondary caries formation in (Group-2).&#x0D; Conclusion: Patients were more approachable for Giomer restoration than Glass ionomer for the management of cervical caries over a period of 12 month.&#x0D; Chatt Maa Shi Hosp Med Coll J; Vol.20 (2); July 2021; Page 60-64

Tissue Conditioner Incorporating a Nano-Sized Surface Pre-Reacted Glass-Ionomer (S-PRG) Filler.

We aimed to evaluate the properties of a novel tissue conditioner containing a surface pre-reacted glass-ionomer (S-PRG) nanofiller. Tissue conditioners containing 0 (control), 2.5, 5, 10, 20, or 30 wt% S-PRG nanofiller or 10 or 20 wt% S-PRG microfiller were prepared. The S-PRG nanofillers and microfillers were observed using scanning electron microscopy. The ion release, acid buffering capacity, detail reproduction, consistency, Shore A0 hardness, surface roughness, and Candida albicans adhesion of the tissue conditioners were examined. The results indicated that the nanofiller particles were smaller and more homogeneous in size than the microfiller particles. In addition, Al, B, F, and Sr ions eluted from S-PRG were generally found to decrease after 1 day. Acid neutralization was confirmed in a concentration-dependent manner. The mechanical properties of tissue conditioners containing S-PRG nanofiller were clinically acceptable according to ISO standard 10139-1:2018, although the surface roughness increased with increasing filler content. Conditioners with 5–30 wt% nanofiller had a sublethal effect on C. albicans and reduced fungal adhesion in vitro. In summary, tissue conditioner containing at least 5 wt% S-PRG nanofiller can reduce C. albicans adhesion and has potential as an alternative soft lining material.

Evaluation of bonding performance and multi-ion release of S-PRG fillercontaining self-adhesive resin composite.

The bonding performance of a surface pre-reacted glass ionomer (S-PRG) filler-containing self-adhesive flowable resin composites to enamel and dentin were evaluated using a tensile bond test with thermal cycling. Also, the quantities of various ions released from the materials were measured using ICP atomic emission spectrometry and a fluoride ion electrode. The initial bond strengths of the materials were approximately 6 MPa, and decreased after thermal cycling. The S-PRG filler-containing self-adhesive flowable resin composites materials exhibited much higher ion release compared with the commercial self-adhesive flowable resin composite possibly due to different acidic monomers contained. It was suggested that the S-PRG filler containing self-adhesive flowable resin composites should be limited as a lining material or to restore small cavities in non-stress bearing areas.

Interactions of boron released from surface pre-reacted glass ionomer with enamel/dentin and its effect on pH

This study investigated the interaction of borate ions released from surface pre-reacted glass ionomer (S-PRG) fillers with the biological apatites of enamel and dentin using solid-state (SS) magic-angle spinning nuclear magnetic resonance (MAS-NMR) spectroscopy analysis. We further evaluated the effect of borate ions on the pH change. Bovine enamel and dentin powder were submerged in S-PRG eluate (with borate ion concentration of 100 mM) for 3 h, then washed with deionized water (DW). The dried enamel and dentin specimens were used for 11B SS-NMR and Fourier transform infrared spectroscopy (FT-IR) analysis. Enamel and dentin blocks were treated with three solutions: DW (control), S-PRG eluent and borate solution (100 mM). The treated samples were submerged in the demineralization solution and the pH was measured using a pH meter daily for 6 days. The surfaces of the enamel and dentin blocks were then observed using field emission scanning electron microscopy (FE-SEM). SS-NMR analysis revealed the presence of adsorbed borate ions in the enamel and dentin samples in a tetra-coordinated form. The pH results demonstrated an increase in pH values in the S-PRG and borate groups. SEM images showed that the surfaces of the control group were demineralized, whereas the surfaces of the S-PRG and borate groups were intact. These results concluded that borate ions could be adsorbed to enamel and dentin in the tetra-coordinated form. Borate ions possess a buffer capacity which may help to protect the tooth structure against acid attacks and promote remineralization.

Pulp tissue reaction to a self-adhesive, resin-based direct pulp capping material containing surface pre-reacted glass-ionomer filler

ObjectiveThis study aimed to evaluate the effect of direct pulp capping using an experimental self-adhesive resin for direct pulp capping (SRD) containing silica and surface pre-reacted glass-ionomer (S-PRG) filler on pulpal healing and to monitor the dentin bridge formation in rat pulp 2–4 weeks after operation. MethodsFive types of SRDs (SRD-0: S-PRG fillers 0 wt%; SRD-1: S-PRG fillers 9.1 wt%; SRD-2: S-PRG fillers 18.4 wt%; SRD-3: S-PRG fillers 27.8 wt%; and SRD-6: S-PRG fillers 57.4 wt%) were prepared, and mineral trioxide aggregate (MTA) was used as control (n = 8). Direct pulp capping was performed on rats that were sacrificed for further evaluation 2 or 4 weeks after the operation. The pulp tissue disorganization (PTD), inflammatory cell infiltration (ICI), and reparative dentin formation were histopathologically evaluated; the data were statistically analyzed using the Kruskal–Wallis and the Mann–Whitney U tests. ResultsThe histopathological evaluation of SRD-1-treated test animals 2 weeks post-operation revealed inferior PTD and ICI when compared with that of MTA. Even 4 weeks after the operation in SRD-1- and SRD-2-treated rats, the PTD and ICI were inferior when compared with those of MTA. The dental specimens of SRD-0 and MTA showed orthodentin formation, whereas SRD-treated test animals showed osteodentin formation at a position slightly deeper than the site of the pulpal exposure. SignificanceThe reparative dentin formed by SRD-0 and MTA was genuine, whereas that formed by SRD-3 and SRD-6 was ossified and ectopic. SRD may have the potential to be utilized clinically as a direct pulp capping material.

The effect of surface pre-reacted glass-ionomer filler eluate on dental pulp cells and mineral deposition on dentin: In vitro study.

The effects of surface pre-reacted glass-ionomer (S-PRG) filler on pulpal cells and on the composition of dentinal deposits were investigated. Proliferation (CCK-8), cytotoxicity (LDH), and differentiation activity (ALP) tests, along with cell morphology observations, were conducted at 6 and 24h after treatment of pulpal cells with different S-PRG filler eluate concentrations. Dentinal surfaces were immersed in deionized water or S-PRG filler eluate followed by immersion in deionized water or simulated body fluid and observed under scanning electron microscope and elemental analysis using energy dispersive x-ray spectrometer. At 24h, there were significant differences in CCK-8 and ALP activity values between the groups in a concentration-dependent manner. LDH test data were not significantly different among the groups. Cell morphology was not altered at either exposure time. However, decreased cellular density was observed with the highest eluate concentration. Crystalline deposits and occluded dentinal tubules were observed in samples immersed in S-PRG filler with a later immersion in simulated body fluid, which also showed higher concentrations of certain ions compared to surfaces that were not initially treated with S-PRG filler. The lowest two eluate concentrations did not show significant toxicity. S-PRG enhanced the effect of simulated body fluid in the formation of mineral deposits.

Evaluation of tooth demineralization and interfacial bacterial penetration around resin composites containing surface pre-reacted glass-ionomer (S-PRG) filler

ObjectiveTo evaluate preventive effects of S-PRG-filled resin-composites on surrounding tooth demineralization and interfacial bacterial penetration. MethodsCavities were prepared on enamel or dentin blocks and treated by BeautiBond Universal (Shofu) and filled by resin-composites containing S-PRG filler (Shofu) at 0 wt% (P0, control), 10 wt% (P10), 30 wt% (P30), 50 wt% (P50) or 70 wt% (P70). The specimens were demineralized by Streptococcus mutans biofilms for 12 days. Wall lesion depth (WL-depth) and outer lesion depth (OL-depth) were measured from fluorescence microscopy. Interfacial bacterial penetration area (BPA) was evaluated from confocal laser scanning microscopy. Wall lesion hardness (WL-hardness) and outer lesion hardness (OL-hardness) were measured with a Vickers microhardness tester. Elemental analysis of resins and surrounding lesions was conducted by scanning electron microscopy and energy dispersive X-ray spectrometry. ResultsResin-composites in P70 contained significantly more F, Na and Al than that in P0 and P10. P70, P50 and P30 showed significantly lower WL-depth than P0. P70 showed significantly lower OL-depth, fewer Ca and P loss in lesions than P0. P70 showed significantly higher WL-hardness and OL-hardness in dentin specimens than P0. Enamel margins had a higher gap frequency than dentin margins. However, BPA did not show significant differences among the groups. SignificanceResin-composites containing S-PRG fillers at ≥30 wt% can inhibit wall lesions and that at 70 wt% can further decrease outer lesions, reduce Ca and P loss in lesions and increase surrounding dentin hardness. However, S-PRG-filled resin-composites does not inhibit interfacial biofilm penetration after a short-term demineralization period.