Surface Of Explants Research Articles

Oxidation of a zirconium nitride multilayer-covered knee implant after two years in clinical use

The surface composition and microstructure of an up to 5 µm thick multilayer on a knee implant were investigated. When the implant was explanted after approximately two years of clinical use due to failure from aseptic loosening, the topmost ZrN layer was found to be oxidized. Interestingly, only the non-articulating area was visibly oxidized (color change).Up until then, the formation and characteristics of the oxide and its influence on the tribological performance remained uncertain. The oxide was thoroughly analyzed using transmission electron microscopy (TEM) and atom probe tomography (APT). The articulating and non-articulating areas were compared with an as-fabricated implant, which served as a reference. The results show that a thin oxide was also present on the articulating surface. All measured oxides were thicker than expected from native oxidation. The oxygen content of the majority of the oxide, measured with energy dispersive X-ray spectroscopy (EDS) and APT, was lower than required for the stable ZrO2 form. Underneath the oxide, the ZrN layer remained unaffected, demonstrating the oxide's effective passivating behavior against further oxidation. No cobalt from the substrate was detected within the ZrN layer, proving the multilayer's excellent barrier function against ion release from the base metal. Statement of significanceIn our aging society, the use of artificial knee implants is widespread. Implant failure is not only costly, but often connected with pain for the patient and inevitably involves the implantation of a new joint. Hence, understanding the origin of joint failure is of high importance to extend the lifetime of the implant. In this research, we investigate the influence of a surface oxide, formed on an explant which failed after ∼ 2 years, on the multilayer stability. As a novelty, we focus not on the wear of the polyethylene gliding surface but on the formed oxide. The use of high-resolution analysis techniques allowed us to get a glimpse on the ongoing mechanisms at the explants surface.

Comprehensive study on explant surface sterilisation of in vitro culture of bamboo species (Bambusa balcooa)

Comprehensive study on explant surface sterilisation of in vitro culture of bamboo species (Bambusa balcooa)

Comprehensive study on in vitro propagation of some imported peach rootstocks: in vitro explant surface sterilization and bud proliferation

The present study was conducted in the Laboratory of Tissue Culture, Horticulture Department, Faculty of Agriculture, Damietta University, Egypt. The objective of this study was to establish a micropropagation protocol suitable for three imported peach rootstocks: Okinawa (P. persica), Nemared (P. persica × P. davidiana) × P. persica), and Garnem (P. dulcis × P. persica) in vitro. The results showed that soaking the explants in sodium hypochlorite (NaOCl) at 20% for 15 min produced the highest responsiveness (82.81%), survival (96.61%), with the lowest mortality (3.14%) and contamination (0.24%). Explants of the Garnem genotype had the best response (89.12%), survival (90.62%), lowest mortality (0.00%), and highest contamination (9.37%) when compared to the other genotypes. In comparison with axillary buds, the shoot tip displayed the highest responsiveness, survival, and death (100, 87.40, and 12.59%, respectively), as well as the least significant contamination (0.00%). Additionally, the percentages of responsive, survived, dead, and contaminated explants at the various collection dates varied significantly. The 6-benzylaminopurine (BAP) concentrations used (3 to 5.0 mg/L) demonstrated similar behavior in terms of in vitro proliferation, with rates of 3.77 to 6.11, 4.33 to 8.88, and 3.33 to 7.44 shoot numbers per explant for the Okinawa, Nemared, and Garnem peach rootstocks, respectively, indicating that the number of shoot proliferations is genotype-dependent. Additionally, using 5.0 mg/L BAP in combination with 0.2 mg/L IBA significantly increased average shoot proliferation (96.29%), number of shoots per explant (7.48), and average leaf number/explant (16.33) compared to the other treatments. Based on these results, adventitious bud development was enhanced during in vitro multiplication of the Okinawa, Nemared, and Garnem peach rootstocks by the synergistic interaction of indole-butyric acid (IBA) and 6-benzylaminopurine (BAP).

Agrobacterium Transformation of Tea Plants (Camellia sinensis (L.) KUNTZE): A Small Experiment with Great Prospects.

Tea has historically been one of the most popular beverages, and it is currently an economically significant crop cultivated in over 50 countries. The Northwestern Caucasus is one of the northernmost regions for industrial tea cultivation worldwide. The domestication of the tea plant in this region took approximately 150 years, during which plantations spreading from the Ozurgeti region in northern Georgia to the southern city of Maykop in Russia. Consequently, tea plantations in the Northern Caucasus can serve as a source of unique genotypes with exceptional cold tolerance. Tea plants are known to be recalcitrant to Agrobacterium-mediated transfection. Research into optimal transfection and regeneration methodologies, as well as the identification of tea varieties with enhanced transformation efficiency, is an advanced strategy for improving tea plant culture. The aim of this study was to search for the optimal Agrobacterium tumefaciens-mediated transfection protocol for the Kolkhida tea variety. As a result of optimizing the transfection medium with potassium phosphate buffer at the stages of pre-inoculation, inoculation and co-cultivation, the restoration of normal morphology and improvement in the attachment of Agrobacterium cells to the surface of tea explants were observed by scanning electron microscopy. And an effective method of high-efficiency Agrobacteria tumefaciens-mediated transfection of the best local tea cultivar, Kolkhida, was demonstrated for the first time.

In vitro propagation of stone fruit rootstock cultivar Evrica 99 and its influence on some phytochemical traits of fresh apricot fruit

Background: The cultivation of stone fruits is of primary importance in Armenia. Their fruits contain antioxidants, fiber, potassium, vitamin A, C, E, minerals, etc., which have a beneficial effect on human health and prevent many diseases. The concentration of those components varies depending on ecological factors, cultivar, rootstock, cultural practices, etc. Clonal rootstocks are important for increasing orchard density, tree uniformity, and high yields, and they can also affect fruit quality. In vitro culture is a valuable method for rapid propagation of high-quality, virus free plant material. Objective: The purpose of this study was to develop in vitro production technology for the stone-fruit rootstock cultivar Evrica 99, and to determine if rootstocks affect some fresh fruit traits of ´Yerevani´ and ´Sateni´ apricot cultivars. Methods: The shoot apical meristem and lateral bud served as explants for shoot regeneration. Different sterilizing agents at various periods of exposure were used for the explant surface sterilization. Various concentrations of phytohormones, both individually and in combinations, were employed for in vitro regeneration and rooting of plants. The titratable acidity (TA), dry matter (DM), vitamin C, mineral content, total carotenoids (TC), and sugar contents were evaluated in fresh fruit. Results: The most optimal option for explant surface sterilization was the gradual application of calcium hypochlorite [Ca (ClO)2] (2.0% solution, exposure time 10 min) and ethanol (70% solution, exposure time 20 s), as a result of which we had 75.5% survival rate of explants. The efficient medium for in vitro shoot regeneration was MS supplemented with 6-Benzylaminopurine (BAP) 0.8 mg/l, Kinetin (Kin) 0.2 mg/l, and Gibberellic acid (GA3) 1.0 mg/l. The half-strength Murashige and Skoog (MS) medium containing 0.8 mg/l indole-3-butyric acid (IBA) was optimal for in vitro rooting. Rooted plants were successfully adapted with a survival rate of 85.0%. The defined method can be successfully used for 'Evrica 99' cultivar micropropagation. The results obtained showed that fruit quality strongly depended on both the varieties and the rootstock tested. Conclusion: In the current study, an alternative in vitro propagation technology for rootstock cultivar ´ Evrica 99 was developed by direct organogenesis, enabling mass-scale production of virus-free plants that is suitable for commercial purposes as well. The apricot cultivars ´Yerevani´ and ´Sateni´ grafted on the virus-free rootstock cultivar Evrica 99 showed higher fruit quality traits, which are essential for human health and diet. Keywords: ´Evrica 99, fucnctional foods, in vitro regeneration, micropropagation, plant growth regulators, stone fruit rootstock, tissue culture.

Combating browning: mechanisms and management strategies in in vitro culture of economic woody plants.

Browning presents a significant challenge in the in vitro culture of economically important woody plants, primarily due to high levels of lignification and the accumulation of secondary metabolites. This phenomenon hampers the development of efficient regeneration and genetic transformation systems across diverse species. This review examines the internal and external factors contributing to browning, including genetic attributes, tree genotypes, physiological state of explants, explant surface sterilization, medium composition, and overall culture conditions. It explores the underlying mechanisms of browning, particularly enzymatic browning caused by the oxidation of phenolic compounds, and highlights the crucial role of redox pathways and phenolic metabolism. Conventional methods for assessing browning, such as sensory evaluation by researchers and the examination of paraffin sections stained with toluidine blue, are commonly used but introduce significant delays and potential biases. The review emphasizes the importance of accurate and timely browning assessment methods, notably the use of Fluorescein diacetate (FDA) staining, as a reliable and quantitative measure of cell viability to better evaluate browning intensity and progression. Additionally, this review explores the potential manipulation of key genes in the phenylpropanoid pathway to lower phenolic biosynthesis. Advanced strategies, such as regenerative gene manipulation and natural product encapsulation, are also discussed for their potential to improve regeneration outcomes. By integrating recent advancements in molecular biology and tissue culture techniques, this review offers novel insights and potential solutions for mitigating browning, thereby enhancing the regeneration capacities of woody plants. This comprehensive approach addresses the mechanistic bases of browning and underscores the importance of optimizing cultural practices and genetic strategies to overcome this challenge.

Gestational Diabetes Mellitus Impedes Fetal Lung Development Through Exosome-Dependent Crosstalk Between Trophoblasts and Lung Epithelial Cells.

Fetal lung underdevelopment (FLUD) is associated with neonatal and childhood severe respiratory diseases, among which gestational diabetes mellitus (GDM) play crucial roles as revealed by recent prevalence studies, yet mechanism underlying GDM-induced FLUD, especially the role of trophoblasts, is not all known. From the perspective of trophoblast-derived exosomes, we established in vitro, ex vivo, in vivo and GDM trophoblast models. Utilizing placenta-derived exosomes (NUB-exos and GDMUB-exos) isolated from normal and GDM umbilical cord blood plasma and trophoblast-derived exosomes (NC-exos and HG-exos) isolated from HTR8/SVneo trophoblasts medium with/without high glucose treatment, we examined their effects on fetal lung development and biological functions. We found that, compared with the NUB-exos group, the exosome concentration increased in GDMUB-exos group, and the content of exosomes also changed evidenced by 61 dysregulated miRNAs. After applying these exosomes to A549 alveolar type II epithelial cells, the proliferation and biological functions were suppressed while the proportion of apoptotic cells was increased as compared to the control. In ex vivo studies, we found that GDMUB-exos showed significant suppression on the growth of the fetal lung explants, where the number of terminal buds and the area of explant surface decreased and shrank. Besides, the expression of Fgf10, Vegfa, Flt-1, Kdr and surfactant proteins A, B, C, and D was downregulated in GDMUB-exos group, whilst Sox9 was upregulated. For in vivo studies, we found significant suppression of fetal lung development in GDMUB-exos group. Importantly, we found consistent alterations when we used NC-exos and HG-exos, suggesting a dominant role of trophoblasts in placenta-derived exosome-induced FLUD. In conclusion, GDM can adversely affect trophoblasts and alter exosome contents, causing crosstalk disorder between trophoblasts and fetal lung epithelial cells and finally leading to FLUD. Findings of this study will shine insight into the theoretical explanation for the pathogenesis of FLUD.

253 Protective and cleansing effect of a skin care product against pollen allergen accumulation in the hair follicle

Environmental airborne elements such as pollutant matters, chemicals, or allergens, such as pollen allergens accumulate on the skin and more particularly in the infundibula of the hair follicle due to its specific form and function. This accumulation can lead to irritation, discomfort or even sensitization and allergic reactions. Thereby, prevention of accumulation or cleansing of these elements within the infundibulum is a key challenge to protect skins submitted to environmental factors. A new study model was set up to mimic pollen allergen deposit onto the skin and test the effect of a skin care product to prevent or cleanse the allergen accumulation. Explants from a hairy abdominoplasty were prepared and placed on Perfex vivo culture supports (Bio-EC). Recombinant allergen Phl p 5b from Timothy grass pollen (abcam) was applied on the surface of explants for 6 hours and the explants were proceeded for immunohistochemistry to visualize the allergen accumulation. Pretreatment with the skin care product was used to evaluate the preventive effect or a cleansing protocol was used to quantify the curative efficacy. The skin care product was able to completely prevent accumulation along the hair follicle when applied on the explant prior to allergen exposition. A strong removal (-83%) of accumulated allergens was observed when explants were cleansed using the same product. In conclusion, a study model was set up to mimic pollen allergens accumulation in the infundibulum. Protective effect thanks to the film forming capacity of the tested skin care product or its cleansing ability could be demonstrated. The product displayed a great double effect, making it an ally to protect skin from environmental airborne elements allowing sensitization processes prevention.

Exogenous implications of silver nitrate on direct and indirect somatic embryogenesis and germination of cold stored synseeds of Vanilla planifolia Jacks. ex Andrews

• Silver nitrate the medium improved somatic embryogenic efficiency in Vanilla planifolia . • Medium containing cytokinins with 2.5 mg L −1 silver nitrate induced maximum number of somatic embryos. • Developmental transition from globular to cotyledonary stages of somatic embryos were confirmed by anatomical studies. • Silver nitrate favored proliferation and rooting of shoots, and improved the survival rate of V. planifolia plantlets. Vanilla planifolia – a natural source of vanillin is the world's highest-valued spice crop with endangered status and the only hand-pollinated crop globally. The present study evaluates the effect of exogenous silver nitrate (AgNO 3 ) on effective direct and indirect somatic embryogenesis in V. planifolia for the first time. The direct and indirect somatic embryogenic capacity of leaf explants were studied using Murashige and Skoog (MS) medium containing 6-benzylaminopurine (BAP), kinetin (KN), 2,4-Dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), and α-naphthalene acetic acid (NAA) alone and in combinations. Additionally, the efficacy of silver nitrate (SN) in promoting somatic embryogenesis was tested with optimized growth regulators. Non-embryogenic callus was obtained from the explants when cultured on 3.0 mg L −1 2,4-D. The embryogenic potential was developed while culturing the non-embryogenic callus on 1.0 mg L −1 each of BAP and KN combined with 2.5 mg L −1 SN. On the other hand, direct somatic embryogenesis (92.0%) was obtained when culturing the explants directly on MS medium containing 1.0 mg L −1 each of BAP and KN combined with 2.5 mg L −1 SN, and a maximum of 30.0 somatic embryos (SEs) were induced per explant. Microscopic analysis revealed that SEs were originated externally as cell division initiated on the leaf surface and cut ends of explants, and have no vascular connections with the explant. Actively dividing embryonic cells possessed dense cytoplasm with prominent nuclei. The mature SEs were encapsulated and stored at - 4 °C for one year. The synseeds showed maximum germination and proliferation (17.2 shoots per SE) on MS medium containing 0.5 mg L −1 BAP, 0.25 mg L −1 KN, and 2.5 mg L −1 SN. The results indicated that AgNO 3 in the medium promoted somatic embryogenesis and plantlet regeneration in V. planifolia .

Sex determination by molecular markers and micropropagation of Actinidia chinensis

In this study, a molecular sex-linked marker was used to determine the sex of 8 kiwifruit plants. In addition, leaf explants with sex determination were used as the original plant material for micropropagation via callus induction, shoot regeneration, and shoot cluster proliferation. The results showed that the sex determination method of Kiwi plants based on molecular markers on the Y chromosome amplified by primer pair SmY1_F/R successfully identified male Kiwi explant belonging to Actinidia chinensis. In addition, optimal explant surface sterilisation and shoot regeneration were obtained from leaf explants disinfected with 200 ppm silver nanoparticles; meanwhile, the culture medium supplemented with 20% coconut water was suitable for the highest efficiency of shoot cluster proliferation.

In vitro propagation and plant regeneration of Torenia crustacea (L.) Charm. & Schltdl; an important ethnic medicinal plant

Torenia crustacea (L.) Charm. & Schltdl is an important medicinal herb used in India, Indonesia and Malaysia. This herb is used by Bodo tribes in the Bodoland Territorial Region, Assam to treat various diseases like diabetes. The present experiment developed an efficient protocol for in vitro mass propagation technique for Torenia crustacea species using its nodal explants. For tissue culture, the rapidly growing nodal explants of Torenia crustacea were used. Effective explant surface sterilisation was found at 15 min. of treatment with 2% sodium hypochlorite resulting in a maximum explant survival rate and a lower rate of explant contamination after 21 days of explant culture. Media containing full strength MS (Murashige and Skoog's) + BAP (6- Benzyl amino purine) (1 mg/l) were found most effective for the establishment of the explant. The highest shoot proliferation and multiplication were observed in the media containing full strength MS + BAP (1 mg/l) + NAA (Naphthalene acetic acid) (0.2 mg/l); in this combination, an average of 16 shoots formed per nodal explant. The rooting of explants was observed highest in MS media along with 0.4 mg/l concentration of NAA and 1 mg/l BA. The in vitro multiplied shoots were grown in plastic pots containing vermicompost fertiliser and soil mixture and successfully grown in the open field condition.

In vitro propagation and somaclonal variation study of Phlogacanthus thyrsiformis Nees an ethnic medicinal plant

Bodoland Territorial Region is very rich in natural bioresources, and the Bodo tribes of the region use numerous ethnic medicinal plants to treat different kinds of diseases. Phlogacanthus thyrsiformis Nees is an important medicinal shrub species used in the region to treat different kinds of diseases. The objectives of the present study were to develop an efficient in vitro mass propagation technique of the species using nodal explant and study genetic stability in the genome of in vitro propagated plantlets by different RAPD markers. Effective explant surface sterilisation resulted at 2 minutes of treatment with 0.1% mercuric chloride. Explant responses were found most effective in the full strength MS + 1mg/l BAP (6- benzyl amino purine), and explant highest shoot proliferation multiplication and rooting were found in the media MS + 1 mg/l BAP + 0.250 mg/l NAA. Ex vitro rooting of micro propagated plants was most effective when the explants were dipped in 1mg/l IBA for an hour. RAPD assays were conducted using eight sets of random primers (OPC02, OPC05, OPC07, OPC08, OPC09, OPX06, DK2 and OPA01). All the primers except OPC07 and OPA01 formed monomorphic DNA bands in gel electrophoresis and polymorphism was detected by OPC07 and OPA01 primer.

Characterization of a Novel Implant Intended to Expand and Reshape the Prostatic Urethra for the Treatment of Benign Prostatic Hyperplasia: A Pre-Clinical Feasibility Study in the Canine Model

ObjectiveTo evaluate the safety, feasibility, and tissue response of a novel device for the treatment of lower urinary tract symptoms secondary to benign prostatic hyperplasia, using the first-generation Urocross Expander System (Mercury Expander System). MethodsThe implant was deployed and retrieved using flexible cystoscope in 8 adult male canines, separated into three study arms by retrieval date (1-, 6-, and 12- months post-deployment). Cystoscopy and urethrograms verified implant position/diameter; bladder neck and external sphincter function/changes; prostatic tissue response; and implant condition. One-month post-retrieval, the prostate and surrounding tissue was sectioned and evaluated by a veterinary pathologist. ResultsAll implants were successfully deployed in the prostatic urethra. Urethral width was increased (6.9 ± 1.8 mm to 10.2 ± 0.6 mm, P = .012) and preserved through the dwell period. Urethral length and sphincter diameters didn't significantly change. All subjects (n = 8) remained continent without obstruction or retention. Adverse events included incisional site bleeding (n = 2) and transient hematuria (n = 3). One implant migrated into the bladder and spontaneously repositioned into the prostatic urethra. Post-retrieval, explant surfaces demonstrated no tissue growth, encrustation or stone formation. Imaging revealed contact site erythema and indentation, but no stones, strictures, perforations, erosions, nor ulcerations. Histopathology revealed glandular acinar changes, inflammation, and fibrosis. ConclusionThe first generation of the Urocross Expander System demonstrated a favorable safety profile in the canine model. Changes in the prostatic urethra shape were noted with an increase in urethral width during the dwell period with minimal tissue changes. Further, the implant didn't demonstrate any encrustation, tissue growth or stone formation.

Silver nanoparticles enhanced efficiency of explant surface disinfection and somatic embryogenesis in Begonia tuberous via thin cell layer culture

In vitro culture establishment is one of the most important stages in micropropagation. The disinfectant effectiveness depends on the type of surface disinfectant, concentration and the time treatment. In this initial study, silver nanoparticles (AgNPs) were used as a disinfectant for petioles, flower stalks and stems of Begonia tuberous. In addition, thin cell layer culture (TCL) technique has been applied for the purpose of somatic embryogenesis. The results showed that AgNPs were effective in eliminating infectious microorganisms on B. tuberous explants; which were identified included 4 species of fungi (Fusarium sp., Aspergillus aculeatus, Trichoderma sp. and Penicillium sp.) and 1 species of bacteria (Pseudomonas sp.). At concentrations of 200 ppm and 300 ppm, AgNPs were not only effective in disinfection but also increased the induction rate of somatic embryogenesis in flower stalk TCL explants (approximately 40.00%); a similar effect was observed in stem TCL explants at the same concentration. Meanwhile, for petiole TCL explants, the induction rate of somatic embryogenesis was optimal when using AgNPs at a concentration of 100 - 300 ppm to disinfected the explant. In contrast, at high (400 ppm) or low (50 ppm) concentrations of AgNPs did not play a disinfecting role and stimulated somatic embryogenesis. In addition, explants derived from AgNPs sterilization did not show any abnormalities in somatic embryogenesis with shapes such as globular, heart, torpedo, and cotyledon. AgNPs showed double efficacy in sterilization of explants and improved efficiency of somatic embryogenesis from TCL petioles, flower stalks and stems explants; thus increasing the efficiency micropropagation of B. tuberous.

THE ESTABLISHMENT OF ASEPTIC CULTURES AND MULTIPLE SHOOT INDUCTION OF OLIVE (Olea europaea) cv.1

Olea europaea or the olive plant is from the family of Oleaceae and has been cultivated for its fruits that are highly nutritious, capable of reducing risks of cardiovascular diseases and to prevent cancer. The current propagation technique of olive plants in Malaysia relies on conventional methods that are less efficient in producing good quality plant stocks. Plant tissue culture offers an alternative to multiply plants from novel mother plants and is commonly applied in the propagation of various crops. This study aims to establish sterile cultures and to induce multiple shoots from Olea europaea cv.1 as a preliminary study to micropropagate olive plants for commercial farms in Malaysia. Nodal explants were surfaced sterilised with ethanol and Clorox© at different durations followed by treatments in MS media supplemented with different concentrations of Zeatin and BAP. Nodal explants surface sterilised with 70% (v/v) ethanol for 2 min and 30% (v/v) Clorox© for 8 min produced sterile explants with the survival rate of 85%. MS media supplemented 5.0 mg/L BAP was optimal for shoot induction (2.10 ± 0.31 shoots per explant) and shoot elongation (6.50 ± 1.17 mm). The current study serves as preliminary assessment for the establishment of local olive cultures.

Micropropagation of Ginger (Zingiber officinale Roscoe) 'Bentong' and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant.

‘Bentong’ ginger is the most popular variety of Zingiber officinale in Malaysia. It is vegetatively propagated and requires a high proportion of rhizomes as starting planting materials. Besides, ginger vegetative propagation using its rhizomes is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied in many plant species to produce their disease-free planting materials. As ‘Bentong’ ginger is less known for its micropropagation, this study was conducted to investigate the effects of Clorox (5.25% sodium hypochlorite (NaOCl)) on explant surface sterilization, effects of plant growth regulators, and basal media on shoots’ multiplication and rooting. The secondary metabolites and antioxidant activities of the micropropagated plants were evaluated in comparison with conventionally propagated plants. Rhizome sprouted buds were effectively sterilized in 70% Clorox for 30 min by obtaining 75% contamination-free explants. Murashige and Skoog (MS) supplemented with 10 µM of zeatin was the suitable medium for shoot multiplication, which resulted in the highest number of shoots per explant (4.28). MS medium supplemented with 7.5 µM 1-naphthaleneacetic acid (NAA) resulted in the highest number of roots per plantlet. The in vitro-rooted plantlets were successfully acclimatized with a 95% survival rate in the ex vitro conditions. The phytochemical analysis showed that total phenolic acid and total flavonoid content and antioxidant activities of the micropropagated plants were not significantly different from the conventionally propagated plants of ‘Bentong’ ginger. In conclusion, the present study’s outcome can be adopted for large-scale propagation of disease-free planting materials of ‘Bentong’ ginger.

The Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%

Protective properties of Avène Thermal Spring Water on biomechanical, ultrastructural and clinical parameters of human skin.

Thermal Spring Water (TSW) has been recognized to have beneficial effects on skin; however, the mechanisms underlying these are not completely elucidated. We compared the effects of Avène TSW with mineral-rich (MR) TSW on the biomechanical properties of the skin using mechanistic exvivo assays and clinical studies. Ex vivo studies included the effect of both TSWs on the structure of the surface of human skin explants using scanning electron microscopy (SEM); mineral elemental content on the skin surface using SEM coupled to energy dispersing X-ray spectroscopy; and the stress properties of the stratum corneum (SC) when exposed to dehydration. Human clinical studies were conducted to compare the soothing effect of TSWs after a dermatological chemical peeling of face skin and to evaluate the overall sensitive scale of consumers using Avène TSW for 7days. Both TSWs preserved surface skin ultrastructure; however, crystals formed from MR-TSW were needle-like and formed small grains, present in clusters heterogeneously spread over the surface. Needle crystals were mainly composed of calcium, while small clusters were mainly composed of sulphur. By contrast, Avène TSW-formed crystals composed of sodium and chlorine only were regular in shape and homogeneously distributed across the skin surface. Peak stress of SC layers was increased by MR-TSW, whereas Avène TSW showed a comparatively reduced effect on dehydration and stress. The difference in the two TSW types was reflected in clinical findings comparing postpeeling redness after TSW application. Avène TSW significantly decreased postpeeling redness, while MR-TSW increased it. The overall sensitive scale of consumers was decreased by 47% using Avène TSW for 7days. Avène TSW decreases postpeeling redness and soothes sensitive skin in human volunteers. Mechanistic studies suggested that differences in biomechanical effects could be linked to differences in calcium content of the TSW.

Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture

The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L Benomyl combined with NaOCl decreased the contamination of explants, and achieved 20% axenic culture.

Bio-inspired apatite particles limit skin penetration of drugs for dermatology applications

Most treatments of skin pathologies involve local administration of active agents. One issue can however be the partial transcutaneous diffusion of the drug to blood circulation, leading to undesirable effects. In this work, the original use of submicron mineral particles based on bio-inspired calcium phosphate apatite was explored for the first time as drug carriers for favoring topical delivery. The permeation of a model drug across synthetic and biological membranes was investigated in both static and dynamic conditions. Our data show that adsorption of the drug on the apatite particles surface drastically limits its permeation, with lower effective diffusion coefficients (Peff) and smaller total released amounts. The retention of the apatite colloidal particles on porcine ear skin explants surface was demonstrated by combining histological observations and Raman confocal microscopy. All results converge to show that association of the drug to apatite particles favors skin surface effects. These findings point to the relevance of mineral-based particles as drug carriers for local delivery to the skin, and open the way to novel applications of bio-inspired apatites in dermatology. Statement of SignificanceCalcium phosphates (CaP) are major biomaterials in orthopedics and dentistry. Their resemblance to bone mineral allows new applications beyond bone repair, e.g. in nanomedicine. In 2018, a 14-page detailed review (M. Epple, Acta Biomaterialia 77 (2018) 1–14) provided clear facts in favor of the non-toxicity of nanosized CaP as an answer to discussions from EU and US study groups, thus clarifying the path to novel applications of nano CaP. In the present paper, bio-inspired apatite nanoparticles are used for the first time as drug carriers for dermatology for drastically limiting drug transcutaneous permeation and retaining a topical effect. We demonstrate this proof of concept via permeation cell tests, histology, Raman microscopy and photoluminescence after application on porcine ear skin.