The sugarcane supply chain is one of the main contributors to economic development in many countries. However, it is necessary to consider the relationship of this supply chain with the environment in order to reduce/mitigate adverse environmental impacts. Life cycle assessment (LCA) supports improving the relationship between production systems and the environment, increasing process efficiency, and reducing costs. The main objective of this research was to identify the extent to which the sugarcane supply chain impacts environmental and human health aspects as well as the use of resources through LCA. This analysis focused on a supply chain in Brazil’s Midwest region, considering the stages of cultivation, transportation, and production. The results indicated that using fertilizers and pesticides and burning crops during harvest severely threaten the sustainability of this supply chain. Also, using crude oil, natural gas, coal, and other chemical products in ethanol production is a critical threat to the sustainability of the sugarcane supply chain. The obtained results cooperate with the decarbonization goals assumed by Brazil at the United Nations Climate Change Conference 2015 (COP 21). A multidimensional approach is necessary to assess the sugarcane production chain results, and the LCA method is appropriate for performing this analysis.
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