The presence of weeds in spinach cultivation land is strongly influenced by the application of fertilizer. Fertilizer has a different content so that it will have an impact on the weed species growth. Identification in this study aims to determine the types of weed species that grow and the dominant types of weed species that grow on spinach cultivation land before and after the application of shrimp waste fertilizer. This research was conducted from June to August 2021, on horticultural crop cultivation in the Sinar Harapan Farmers Group, Tarakan City. The sampling method was a random method using a square plot method with a size of 1 x 1 m as many as 30 samples before and after the application of shrimp waste fertilizer. Observation parameters are counting the number of weed species and the name of the weed species. The data obtained in the field is then processed to determine the level of density, frequency, significant value index (INP), Summed Dominance Ratio (SDR), Margalef Index, Shanon-Wiener Index, Evennes Index and Sorensen Index. The results showed that the weed species before the application of shrimp waste fertilizer on spinach plants were 27 species with the dominant weed species being Cyperus compressus with a Summed Dominance Ratio value of 22.72%. Weed species before the application of shrimp waste fertilizer on spinach plants were 25 species with the dominant weed species namely Cyperus compressus with a Summed Dominance Ratio value of 20.51%. The Margalef index before the application of shrimp waste fertilizer was 3.20 and after the application of shrimp waste fertilizer was 3.05. The Shanon-Wiener index before the application of shrimp waste fertilizer was 2.60 and after the application of shrimp waste fertilizer was 2.40. Evennes index before the application of shrimp waste fertilizer is 0.79 and after the application of shrimp waste fertilizer is 0.74 and the Sorensen index is 85%
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