Aqueous zinc-ion batteries are a promising next-generation energy storage technology. However, the practical application is severely restricted by issues including dendrite growth, corrosion, the hydrogen evolution reaction of zinc anodes, and the failure of the cathode. Traditional electrolyte engineering mainly focuses on weak acidic environments, while neglecting the alkaline byproducts during calendar aging and operation, as well as the synergistic effects between cathode and anode. Herein, we incorporate 5-Sulfosalicylic Acid (SSA) into ZnSO4 (ZSO) electrolyte as a multifunctional additive to co-stabilize both electrodes. SSA not only stabilizes the interfacial pH environment through neutralization of alkaline byproducts but also guides the oriented deposition on the Zn (002) plane due to adsorption. Besides, strong acidic chemistry can inhibit the deprotonation of the polyaniline (PANI) cathode, which increases electrochemical performance. The SSA additive improves the Zn plating/stripping reversibility with a Coulombic efficiency around 99.8% at 1 mA cm-2 for over 2000 h. Besides, the Zn//PANI full cells demonstrate outstanding rate performance and long-term cycling stability, maintaining 72% capacity retention after 1500 cycles at 3 A g-1. The pouch cells perform well for 200 cycles under high mass loading (8 mg cm-2) and lean electrolyte (6.25 µL mg-1) conditions.

ABSTRACT This study introduces a novel spectrophotometric method to sensitively and specifically determine the peroxide value of edible oils, specifically engineered to overcome the significant analytical interference of carotenoid pigments that have historically compromised traditional methods. The method uses a solution containing ferrous (Fe 2+ ) ions and either salicylic acid (SA) or sulfosalicylic acid (SSA). Next, these Fe 2+ ions are reacted with a sample of edible oil in the presence of peroxide, forming ferric (Fe³⁺) ions. In the next step, the Fe³⁺ ion forms a complex with SSA (or SA), resulting in a ferrisulfosalicylate (or sulfosalicylate) complex that exhibits a unique color. This color change was utilized for measuring lipid peroxides. It provides a rapid, simple, and sensitive method for detecting and quantifying peroxides. SSA and SA can form complexes that absorb light at 505 and 525 nm, respectively. When this new method was compared to Ferrous Oxidation‐Xylenol Orange (FOX) and ferrithiocyanate methods, it showed an impressive correlation (Pearson's r = 0.99). This study demonstrates that the proposed method is effective in determining the peroxide value in the various types of edible oils examined.

Sensitive paper-based test strips for quantitative determination of iron (Fe2+) in foodstuffs using optimized colorimetric reagents.

Organic molecule-tailored plasmonic nanoparticles show potential for fabricating an efficient, highly sensitive, and selective detection tool. This approach utilizes surface-enhanced Raman scattering (SERS) that amplifies the scattering from molecules attached to nanostructured metallic surfaces and marks a significant milestone in the development of spectroscopic and analytical methods. For this, silver nanoparticles were synthesized and functionalized with 5-sulphosalicylic acid (5-SSA@AgNPs) using chemical reduction method and characterized with ultraviolet-visible spectroscopy, X-ray diffraction, and Raman spectroscopy. Synthesized 5-SSA@AgNPs was used as a probe for the detection of different metal ions spiked in aqueous solution by distinctive spectral signatures of metal ions using SERS technique. The carboxyl, hydroxyl, and sulfonyl group attached in 5-sulphosalicylic acid resulted in the surface modification of silver nanoparticles and provided stability to the synthesized nanoparticles from agglomeration. The 5-SSA@AgNPs were employed for the identification and quantification of selenium (IV) ions in aqueous solution. The analysis of the Raman signal on addition of 5-SSA@AgNPs and metal ions exhibited peculiar Raman bands of the Se(IV) that facilitated its detection in water samples. The detection limit and quantification limit were 1.08 and 3.60 µM (R2 = 0.95), respectively. Further, this method was utilized for the determination of Se(IV) in water samples, yielding recoveries between 96% and 123%, with relative standard deviations below 5.1% (n = 3). The acquired Raman characteristics demonstrate the capability to provide an economical, enduring, accessible, user-friendly, precise, and prompt method for identifying Se(IV) ion in water-based solutions.

Highly Sensitive Label-Free Immunosensor based on 5-Sulfosalicylic Acid Doped Poly(2-chloroaniline) for Detection of CA72-4 in Gastric Cancer.

Sensitive enzymatic cycling assay for glutathione and glutathione disulfide content.

BackgroundMultiple myeloma patients may present with spuriously elevated serum phosphate levels resulting from the presence of paraproteins interfering in the phosphomolybdate UV assay. If this phenomenon is not recognized, patients possibly receive unnecessary treatments. This short report highlights the existence of paraprotein-related pseudohyperphosphatemia, and aims to provide accessible solutions to eliminate this interference.Material and MethodsIn a patient known with IgG multiple myeloma and unexplained hyperphosphatemia, the correlation between serum phosphate levels (phosphomolybdate UV assay) and IgG concentrations (immunoturbidimetry) was evaluated. To investigate the effect of the paraprotein on phosphate levels, phosphate was measured in one serum sample before and after protein removal by either dilution, protein precipitation with sulfosalicylic acid or zinc sulphate, or ultrafiltration.ResultsA patient with multiple myeloma presented with an unexplained hyperphosphatemia which correlated positively with serum IgG concentrations. As serum dilution normalized the phosphate level, it was hypothesized that precipitation of the paraprotein during the assay reaction interfered with the measurement and resulted in pseudohyperphosphatemia. Protein removal by precipitation with sulfosalicylic acid or zinc sulphate efficiently reduced the IgG level below the detection limit but did not result in a reliable phosphate measurement. Successful removal of proteins and a serum phosphate level that matched the patient's other biochemistry parameters and clinical condition were obtained by ultrafiltration.ConclusionParaproteins can interfere with the reaction components in the phosphomolybdate UV assay and result in pseudohyperphosphatemia. If the presence of this phenomenon is established, a reliable phosphate concentration can be obtained after ultrafiltration of the sample.

The object of this study is the physicochemical processes of interaction between Group II metal ions and polyethyleneimine (PEI) and low-molecular-weight ligands (salicylic acid, sulfosalicylic acid, EDTA) with the aim of developing polyadsorbents for the selective extraction of toxic ions from aqueous media. The problem to be solved is the development of effective and selective polyadsorbents for removing toxic metal ions from wastewater. Potentiometric, conductometric, and viscometric analysis methods have established that in binary PEI-M2+ systems, coordination complexes are formed with a molar ratio of PEI:M2+ = 2:1. For systems with Sr2+ and Ba2+ ions, stepwise complexation was identified, with particles of composition 6:1 and 4:1 being formed at the initial stage, respectively. The stability of the complexes formed was established in the following order: Be2+ > Ca2+ > Sr2+ > Ba2+ > Mg2+. Thermodynamic parameters showed that the complexation processes are spontaneous and exothermic, with the decrease in entropy being associated with an increase in order within the system. Stability constants (lgβ0) range from 5.4 (Mg2+) to 10.16 (Be2+). Quantum chemical calculations have shown that the reaction centers in the ligand molecules are: the oxygen atoms of the carbonyl group (H2Sal), the sulfonate group (H3Ssal), and the carboxyl group (H2edta2⁻). It has been established that donor-acceptor interactions predominate in PEI-M2+-H2Sal/H3Ssal ternary systems, while electrostatic interactions dominate in PEI-Zn2+/Cd2+-H2Sal and PEI-Be2+-H2edta2⁻ systems. In sorption tests in model solutions, the ternary complexes PEI-Be2+-H3Ssal and PEI-Hg2+-H3Ssal demonstrated the highest efficiency: the degree of removal of Be2+ reached 97.1%, Hg2+ – 93.4%, while for Mg2+ and Ba2+ it was less than 70%. Modified PEI with H3sal proved to be the most effective adsorbent among those studied. In industrial trials, the developed adsorbents reduced the concentrations of Be2+ and Hg2+ in wastewater to 0.004 and 0.002 mg/l, respectively, which meets the maximum permissible concentrations for discharge into water bodies

BackgroundThe clinical differentiation between obstetric antiphospholipid syndrome (OAPS) and undifferentiated connective tissue disease (UCTD) presents significant diagnostic challenges. This study employs metabolomics to investigate metabolic reprogramming patterns in OAPS and UCTD, aiming to identify potential biomarkers for early diagnosis.MethodsUsing LC-MS-based metabolomics, we analyzed serum profiles from 40 OAPS patients (B1), 30 OAPS + UCTD patients (B2), 27 UCTD patients (B3), and 30 healthy controls (A1). Multivariate PLS-DA modeling, combined with KEGG pathway and Gene Set Enrichment Analysis (GSEA), was applied to identify disease-specific metabolic signatures.ResultsMetabolomic profiling detected 1,227 metabolites, including 412 in negative ion mode and 815 in positive ion mode. The two ionization modes exhibited distinct chemical profiles, with PLS-DA analysis demonstrating superior group discrimination in positive ion mode. B1 vs B2 (Negative ion mode): nine metabolites were upregulated (notably 17(S)-HpDHA, showing the largest fold-change as a potential biomarker), and one metabolite was downregulated (5-sulfosalicylic acid). B1 vs B2 (Positive ion mode): 17 metabolites were upregulated (including 4-methyl-5-thiazoleethanol, a promising biomarker), and eight were downregulated. B1 vs B3 (Negative ion mode): 14 metabolites were upregulated (highlighted by 3-hydroxybenzoic acid, the most significantly altered candidate), and four were downregulated. B1 vs B3 (Positive ion mode): 30 metabolites were upregulated (again featuring 4-methyl-5-thiazoleethanol), and 32 were downregulated. B2 vs B3 (Negative ion mode): 15 metabolites were upregulated (e.g., chlortetracycline), and 15 were downregulated (notably 6α-prostaglandin I1). B2 vs B3 (Positive ion mode): 29 metabolites were upregulated (e.g., senecionine), and 64 were downregulated (e.g., SM 9:1 2O/16:4). These metabolites represent robust candidates for group discrimination. Enrichment analysis revealed that distinct metabolic pathways were significantly associated with different groups and ionization modes, suggesting divergent underlying metabolic mechanisms.ConclusionThis study systematically characterizes the metabolic reprogramming in OAPS, UCTD, and their comorbid states, identifying potential diagnostic biomarkers. Differential metabolites and pathway analyses highlight the critical role of immunity, contributing to a theoretical framework for “metabolism-immunity-vascular” interactions.

A sensing strategy to significantly enhance the aggregation-induced emission (AIE) of berberine hydrochloride (BBR) is proposed.A supramolecular framework material was prepared by using 5-sulfosalicylic acid (SSA) and nor-seco-cucurbit[10]uril (ns-Q[10]). Single crystal structure analysis confirmed that the SSA@ns-Q[10] assembly forms a ternary complex. SSA@ns-Q[10] assembly can induce BBR aggregation to a large extent, which leads to the enhancement of fluorescence intensity of mixed solution. The results showed that SSA@ns-Q[10] assembly can be used as an excellent fluorescent sensing material for detecting BBR. This assembly has been successfully applied to thedetection of BBR in human serum samples, highlighting its potential for use in both environmental and biological systems. Additionally, the SSA@ns-Q[10]‒BBR system shows promise for a variety of applications, including anti-counterfeiting, screen printing, and the production of fluorescent-emitting handicrafts.

Nitrogen and sulfur co-doped yellow-green fluorescent carbon dots (N,SCDs) were synthesized using thiourea and 5-sulfo salicylic acid as raw materials through a one-step microwave solid-phase method. A series of characterizations, including transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared (FT-IR), UV-Vis spectrum, and fluorescent spectrum, were conducted, revealing that N,SCDs are carbon dots with an average size of approximately 5.65 nm. These dots were found to possess a surface rich in hydroxyl, amino, carbonyl, and sulfhydryl groups, along with excellent solid-state luminescent properties. N,SCDs were combined with starch to form N,SCDs-starch composite, which was successfully utilized for high-quality fingerprint development. Additionally, the solid-state N,SCDs were dissolved in DMSO to produce the liquid form, L-N,SCDs, which was effectively applied for the analysis and detection of Fe3+ and OH-. The limits of detection (LODs) for Fe3+ were determined to be 0.73 and 2.65 μM in the concentration ranges of 0-100 and 100-237.5 μM, respectively. Similarly, the LODs for OH- were found to be 0.027 and 0.66 μM in the ranges of 0-225and 225-525 μM, respectively. Furthermore, L-N,SCDs configured as fluorescent inks were demonstrated to exhibit significant potential in applications related to information anticounterfeiting.

Plywood on phenol-formaldehyde binder is used in carriage building, construction, finishing of small-tonnage vessels and yachts and is an effective substitute for wood. Improving the competitiveness of plywood is possible by reducing the consumption of the binder or decreasing the pressing temperature. In this work, the temperature of hot pressing of plywood FSF and the consumption of the binder were simultaneously reduced. Sulfosalicylic acid was used as a modifier. IR spectra of the binder and plywood were obtained on a Nicolet iS10 IR Fourier spectrometer. In the modified binder and plywood based on it, the amount of bound water and hydroxymethyl groups decreases, the num-ber of CH2 groups increases. At the second stage, the study of the mechanical properties of plywood was carried out by implementing an experiment according to the 2nd order B-plan. Regression models were developed for the strength of plywood under static bending and the strength when shearing along the adhesive layer after boiling for 1 hour. The fol-lowing factors were varied: pressing temperature (X1) from 85 to 105 °C, resin consumption (X2) from 88 to 98 g/m2, modifier additive share (X3) from 0 to 1 %. Reducing resin consumption to 88 g/m2 and decreasing plywood pressing temperature to 85 °C on an unmodified binder provides the material static bending strength limit of 76.4 MPa, while maximally reducing adhesive strength - 0.05 MPa. Plywood samples are destroyed under a load of 20...24 kgf when tested for chipping after boiling, chipping occurs along the adhesive layer. Plywood made with a modified binder at a pressing temperature of 95 °C, a resin consumption of 93 g/m2 of resin surface and a sulfosalicylic acid consumption of 0.5% of the resin weight has the following indicators: shear strength along the adhesive layer after boiling - 0.748 MPa, strength of samples under static bending - 111.57 MPa.

The reactions of various heterocyclic primary amines with sulfonyl chloride derivatives or with sulfosalicylic acid results in the first case to the formation of sulfonamide derivatives and in the second case to the formation of sulfonate organic salts which are both hybrid molecule that integrate multiple pharmacophores into a single scaffold. The novel sulfonamide derivatives 3 were obtained through mono-nucleophilic addition reactions of the corresponding primary amines to the appropriate sulfonyl derivative. In contrast, the sulfonate organic salts 5, 7 and 8 were formed as the result of an acid-base neutralization reaction, during which a molecule of amine captures the acid proton from the sulfosalicylic acid molecule. The antioxidant activity of the synthesized compounds is evaluated by determining their DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radical scavenging activity as well as gallic acid equivalent antioxidant capacity (GEAC). The structures of compounds 3, 5, 7 and 8 were confirmed through elemental analysis, IR (Infrared), UV-Vis (ultraviolet-visible), 1H-NMR (Proton nuclear magnetic resonance), 13C-NMR (Carbon-13 nuclear magnetic resonance) and HRMS (High-Resolution Mass Spectrometry) spectroscopic data. Organic salt 7a was characterized by single-crystal and X-ray diffraction. Organic sulfonate salts were found to be more active than covalent sulfonamide derivatives.

Acute lymphoblastic leukemia (ALL) is the predominant malignancy in pediatric patients. As a crucial constituent of ALL chemotherapy, l -asparaginase is recognized as an integral element of treatment with a threshold concentration of 0.1 IU/mL used in treatment protocols. This study presents a novel liquid chromatography-tandem mass spectrometry method for evaluating plasma l -asparaginase activity in pediatric patients with ALL undergoing pegaspargase therapy. Initially, an enzyme incubation was conducted using 20 μL of plasma and 100 μL of l -asparagine (0.1 mol/L) at 37°C for 15 minutes. The reaction was stopped by adding sulfosalicylic acid and methanol. After the addition of isotope internal standard l -aspartic 13 C 4 acid, subsequent centrifugation, and dilution, the plasma samples underwent analysis by liquid chromatography-tandem mass spectrometry on a hydrophilic interaction liquid chromatography analytical column. A flow rate of 0.3 mL/min was used during isocratic elution using a mobile phase consisting of methanol-water (95:5, vol/vol) with 0.2% formic acid within a 5-minute run. The calibration curves exhibited excellent linearity ranging from 0.1 to 15 IU/mL, with determination coefficients ( r2 ) exceeding 0.99. The precision and accuracy ranged from 1% to 7% and from 93% to 110%, respectively. The relative recovery fell within the range of 98%-100%, and the internal standard-normalized matrix effect ranged from 95% to 101%. The stability was satisfactory across various conditions. This method was fully validated and successfully applied to quantify l -asparaginase activity in plasma samples of 15 children with ALL, enabling the monitoring of l -asparaginase activity with mass spectrometry.

ABSTRACTThree new coordination polymers (CPs) formulated—[Cu(5‐SSA)Cu2(MEA)2]·2H2O (1) (where 5‐SSA = 5‐sulfosalicylic acid, MEA = monoethanolamine); [Mn(SSA)4(H2O)2]n (MEA)(H2O) (2); [Na (SSA)]MEA (3)—have been prepared and characterized by various spectroscopic techniques, elemental analysis, and single‐crystal X‐ray diffraction method. In 1, coordination numbers of both copper ions are six with octahedron geometry. Further, this compound has complicated intermolecular H‐bonds in the polymeric structure that incorporate polymeric chains into 3‐D networks. Compound 2 also exhibits a polymeric bimolecular chain structure and features two intramolecular hydrogen bonds, both having the same graph‐set notation of S11(6) in the structure. Further, it is a supramolecular compound (clathrate or host‐guest complex) with inclusion of monoethanolamine (MEA) and water. Similarly, 2‐D polymeric layer structure of compound 3 with strong H‐bonds generated a 3‐D network. All these CPs were explored to study their cytotoxicity, antiradical activity, and adsorption properties. The cytotoxicity results indicate that compound 1 has acute toxicity in the mice, characterized by adverse effects and a significant mortality rate. Further, the average lethal dose (LD50) of the compounds 1–3 is estimated 546, 708, and 750 mg/kg, respectively. In case of antiradical activity, the CPs 2 and 3 have high antioxidant radical forming (ARF) capability. The adsorption of N₂ at low temperatures was evaluated using an Autosorb device and the measured P0 value at 77 K was 101.3 kPa. In addition, the surface area of these compounds were also determined by Brunauer–Emmett–Teller (BET), which affirmed 311.9, 526.69, and 349.8781 m2/g for compounds 1–3, respectively. The nitrogen adsorption data revealed that compounds 1 and 2 contain only mesopores, while compound 3 consists exclusively of micropores. Barrett–Joyner–Halenda (BJH) method was explored to determine mesopore volume with values of 0.171459, 0.808656, and 0.11969 cm3/g for compounds 1–3, respectively.

The resurgence of interest in lanthanide coordination complexes due to their diverse applications has been increasing for past decade. These lanthanide complexes have found applications such as catalysts, sensors, optoelectronics, light sources, and modified semiconductors. The modifications in the lanthanide complex chemistry due to the use of different complexation agents enhanced the luminescence properties by varying the emission bands, stoke shifts, and lifetimes accordingly. This versatile nature of lanthanide complexes increased our interest to make a good contribution to the field. The complexes of lanthanum, europium, and novel binary complex of La and Eu metals with 1,10-phenanthroline (Phen) and 5-sulfosalicylic acid (SSA) were synthesized by co-precipitation method. Different physicochemical techniques like analytical and spectroscopic were used to characterize the ligands and complexes. The coordination of ligands with metals in complexes was confirmed by FTIR and UV spectroscopy. The surface morphology of nine coordinated La-complexes and seven coordinated Eu complexes was investigated by SEM micrographs. The X-ray crystallographic analysis revealed that all the complexes exhibit a triclinic crystal system. All the complexes were found to exhibit self-reversed magnetic hysteresis (SRMH). The luminescence and magnetic properties of mixed metal complexes are enhanced as compared to their mononuclear analogs.

The hydration behavior and structural fittings of 5- Sulphosalicylic Acid + aqueous-(0.025, 0.050, 0.100) mol·kg-1KCl/CuCl2 solutions were studied by volumetric, acoustical and optical properties form measured densities and ultrasonic velocities at 303.15 K and 101 kPa. Partial molar volumes and compressibilities of 5- Sulphosalicylic Acid in aqueous-KCl/CuCl2 solutions were determined. In addition to this, the refractive indices of studied systems were measured. Significant changes in partial molar volumes and compressions of 5- Sulphosalicylic Acid were observed in presence of KCl/CuCl2. The measured and derived properties were interpreted in terms of ion-ion and ion-dipole in studied solutions. Volumetric, acoustical and optical properties indicated significant effect on hydration structure of 5- Sulphosalicylic Acid in regard with molecular interactions.

In this work, the polythermal solubility of the three-component system containing C7H6 O6S∙2H2 O - NH2C2H4OH - H2O was studied in the visual polythermic method based on binary systems and internal sections in the temperature range from -50.2°C to 42.0°C, and the diagram was constructed. In the solubility diagram, based on the phase-separating binary and ternary points the table was created, and the projection was drawn. In the diagram, the crystallization areas of ice, C7H6O6S, C7H6O6S∙2H2O, NH2C2H4OH∙2H2O, NH2C2H4OH∙H2O, NH2C2H4OH, and a new chemical compound C7H6O6S∙NH2C2H4OH were separated. A new phase formed during the study of the C7H6O6S∙2H2O - NH2C2H4OH - H2O system was separated, and the separated phase was analysed using chemical, physico-chemical methods and the presence of a new chemical compound (C7H6O6S ∙ NH2C2H4OH) was confirmed.

Synthesis, characterization and luminescent properties of silver(I) complexes with 5-sulfosalicylic acid and imidazole derivatives

A novel and simple sensor was constructed based on the glassy carbon electrode (GCE) modified with poly-alanine/sulfosalicylic acid layer-by-layer composite film by electrochemical deposition method, and the electrochemical behavior of dopamine (DA) on the electrode was studied in detail by using cyclic voltammetry and differential pulse voltammetry. Under the optimal conditions, the poly-alanine/sulfosalicylic acid/GCE exhibited a higher catalytic activity for dopamine compared with poly-sulfosalicylic acid/GCE, poly-alanine/GCE and bare GCE, which was attributed to its good synergistic effect between alanine and sulfosalicylic acid and the electrostatic attraction between sulfonate groups and dopamine cations. The detection limit of the composite film modified electrode for dopamine was 0.12 μmol/L with a linear range from 0.7 to 300 μmol/L. At the same time, the diffusion coefficient was estimated to be 6.16×10-6 cm2/s and the number of the electrons transferred during the heterogeneous reaction was calculated as 1.9, indicating that the redox reaction of dopamine on modified electrode involved two protons and two electrons. Moreover, the sensor was not interfered by ascorbic acid (AA) and uric acid (UA), and the peak potentials of AA, DA, UA were 0.109V, 0.281V and 0.427V, respectively, which was vital to improve the selectivity of the sensor for DA detection. The prepared electrochemical sensor was successfully used to detect dopamine in pharmaceutical dopamine hydrochloride injection with satisfactory results.