Suitable Extraction Research Articles

Phytochemical analysis and anti-allergic activity of a combined herbal medicine based on bur-marigold, calendula and hawthorn

Using an experimentally selected extractant for balanced extraction of flavonoids, polysaccharides and polyphenols from bur-marigold herb, calendula flowers and hawthorn leaves and flowers, 3 suitable dry extracts and 1 combined extract in the selected ratio of raw materials were obtained. It was concluded that it was reasonable to standardize the combined herbal extract on the content of flavonoids in terms of luteolin-7-glucoside and content of polyphenols in terms of pyrogallol by UV method and the content of terpenoid compounds, in terms of oleanolic acid by densitometric method. The combined extract at a dose of 30 mg/kg in the model of anaphylactic shock has showed antiallergic activity at the level of tesalin and exceeds diazoline; the ability of the combined extract to reduce the permeability of skin capillaries at the level of the tesalin has established on the model of active cutaneous anaphylaxis and it significantly exceeded diazoline; the combined extract did not differ in membrane-stabilizing properties from the effect of desloratadine and was significantly superior to the reference drugs diazolin and tesalin in the test of indirect degranulation of mast cells. As a result, it was concluded that the mechanism of anti-allergic action of the combined extract is probably associated not only with antihistamine properties but also with the ability to stabilize cell membranes.

Critical evaluation of heat extraction temperature on soluble microbial products (SMP) and extracellular polymeric substances (EPS) quantification in wastewater processes.

While soluble microbial products (SMP) and extracellular polymeric substances (EPS) in wastewater bioprocesses have been widely studied, a lack of standard quantification procedures make it difficult to compare results between studies. This study investigated the effect of temperature on SMP and EPS profiles for biological nutrient removal (BNR) sludges and aerobic membrane bioreactor sludge by adapting the commonly used heat extraction and centrifugation scheme, followed by colorimetric quantification of the carbohydrate and protein fractions using the phenol-sulfuric acid (PS) and the bicinchoninic acid (BCA) methods, respectively. To overcome known inconsistencies in colorimetry, total carbon (TC), total nitrogen (TN), and fluorometry analyses were performed in tandem. SMP samples marginally benefitted from heat extraction, owing to their mostly soluble nature, while EPS profiles were greatly influenced by temperature. 60 °C appears to be a suitable general-purpose extraction temperature near the lysis threshold for the sludges tested. The PS method's misestimation due to lack of specificity was observed and contrasted by TC analyses, while the TN analyses corroborated the BCA assays. Fluorometry proved to be a sensitive and rapid analytical method that provided semi-quantitative information on SMP and EPS constituents, particularly its proteinaceous components, with positive implications for robust wastewater process control.

Magnesium sulfate as a potential dye additive for chlorophyll-based organic sensitiser of the dye-sensitised solar cell (DSSC)

In this work, a new chlorophyll dye-sensitiser derived from mitragyna speciosa (MS) leaves, also known as Kratom, was employed for dye-sensitised solar cells (DSSCs). The influence of magnesium sulfate (MgSO4), a low-cost dye additive, and suitable extraction solvents on the performance of DSSCs were examined. Here, the optical properties were investigated using UV–Visible spectroscopy and the functional anchoring group were investigated by FTIR spectroscopy. Meanwhile, the photovoltaic parameters were investigated by I-V measurements. The highest conversion efficiency is obtained when using a dye extracted from methanol solvent in combination with MgSO4 additive, namely methanolic magnesium sulfate (MMSO). This higher power conversion efficiency is mainly attributed to the enhancement of the hydroxyl group in the MMSO dye solutions, which promotes higher dye adsorption and provides an organic dye passivation layer that reduces back-recombination in the cell. Furthermore, MgSO4 aids in the replenishment of magnesium lost in the chlorophyll porphyrin ring during the degradation process. These combined effects have contributed to the overall conversion efficiency of the MMSO cell at 0.26 %, followed by 0.24 % for ethanolic magnesium sulfate (EMSO), respectively.

Impact of extraction methods on functional properties and extraction kinetic of insoluble dietary fiber from green pea peels: A comparative analysis

The present study focused on the utilization of green pea peel (GPP) by-product for its insoluble dietary fiber (IDF) which was estimated by employing different extraction techniques. A comparative evaluation using various extraction methods viz., alkaline extraction, ultrasound-assisted extraction, and ultrasound-assisted alkaline extraction method (ODF) was employed vis-à-vis its effect on yield, kinetics, thermal stability, morphological, and functional properties of IDF. Ultrasound-assisted alkali extraction method was optimized to extract IDF and was found to be the best extraction method and standardized using rotatable central composite design with time of 30 min, amplitude of 30%, solid to liquid ratio (1:20), and NaOH concentration 1.2 mol/L. ODF evinced biologically rich and higher water and oil holding capacities with low peak and final transition temperature. The morphology of IDF also varied distinctly with extraction processes. ODF conclusively evidenced that it has immense potential to scale up in fulfilling the commercial demand of IDF. Novelty impact statement The pea peel insoluble dietary fiber (IDF) has enormous potential in food processing industries in developing functional foods which could unleash health benefit properties. The utilization could also minimize the environmental footprint of food wastage and disposal issues. The developed IDF from green pea peel (GPP) has enormous economic feasibility among various commercial food sectors viz., beverage, dairy, meat, and other health-promoting food industries. Extraction of IDF by a suitable extraction method from GPP by-product which was discarded largely hitherto has immense potential of generating substantial economic benefits every year and it can be an innovative approach for generating functional food ingredients.

Multicomponent bioactive extract from red stage Haematococcus pluvialis wet paste: avoiding the drying step and toxic solvents

This study provides an efficient alternative by extracting bioactive compounds from Haematococcus pluvialis via matrix solid-phase dispersion (MSPD) from its wet form, reducing one of the process steps with the greatest economic impact, the drying of the microalga. To obtain a suitable extract for nutraceutical purposes, solvents with the generally recognised as safe (GRAS) designation (ethanol, ethyl lactate, and ethyl acetate) with limitations of use (acetone) and extractants with higher toxicity such as methanol and methyl-tert-butyl ether (MTBE) are contrasted. Through the optimisation of the extractive process, ethanol, a GRAS solvent, presents the best overall recovery for carotenoid compounds and fatty acids, showing an antioxidant activity of 1.58 mmolTE g−1 DW, comparable to its synthetic alternative of petrochemical origin without the drawback of having limitations in its food use. In addition, the identification of the phenolic compounds, phloroglucinol, p-coumaric acid, gallic acid, and catechin, not previously characterised in red stage H. pluvialis, provides a response to the phenolic activity present in the extract (24.65 mmolGAE g−1 DW). Comparison of the extractive efficiency obtained with the main methods for the extraction of carotenoids and fatty acids in H. pluvialis, in contrast to the proposed method, shows a positive feasibility of this approach.

Effects of Some Extraction Parameters on Anthocyanin Content of Barberry (Berberis vulgaris L.) and Its Antioxidant Activity

In the study, determination of the optimal conditions for anthocyanin extraction from the dried fruit of barberry (Berberis vulgaris L.) was aimed. For this purpose, the solvent extraction method was used. The present investigation was carried out to different extraction conditions such as different solvents (ethanol and 2% hydrochloric acid, 2% acetic acid and 2% citric acid), the concentration of ethanol (20-80%), the concentration of suitable acid (1-4%), extraction temperature (30-60 oC), extraction time (60-240 min) and raw material and solvent ratio (1:5-1:20). The obtained extract was subjected to total phenolic content and antioxidant activity using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The optimum condition for anthocyanin extraction from barberry extract was as follows: using 80% ethanol and 2% citric acid (100:10) as a solvent, the extraction temperature was 30 oC, extraction time was 120 min and the rate of fruit/solvent ratio was 1:20. In these conditions, the total anthocyanin content, the total phenolic content and antioxidant capacity were determined as 101.03±1.89 mg 100g-1 FW, 3269.05±111.11 mg gallic acid kg-1 and 92.41±0.25%, respectively. Antioxidant activity of barberry (B. vulgaris L.) extracts has been attributed to the high polyphenol content.

Selective recovery of nickel from obsolete mobile phone PCBs

In the present investigation, the selective recovery of nickel from a leach solution of delaminated metal clads from obsolete mobile phone PCBs using the industrial reagent ACORGA M5640 has been studied. As a first step, copper is selectively separated from the mixed metal leach liquor by solvent extraction at pH 2, to leave a nickel-rich raffinate solution. A second stage solvent extraction process conducted at pH 8 then permits recovery of the nickel. Small quantities of zinc and cadmium are also co-extracted. In this study, the conditions for most suitable nickel extraction and stripping have been explored. Results indicate that quantitative extraction of nickel (99.7%) can be achieved using a 1:5 organic (10 vol% extractant in kerosene) to aqueous (pH 8) phase ratio in 60 min. The nickel in the organic phase is readily stripped from the extractant, with more than 95% recovered, along with low levels of zinc (1.7 mg/L) and cadmium (0.6 mg/L), following a 0.5 M hydrochloric acid or 1 M nitric acid strip step. Cyclic usage of the extractant organic layer revealed that its effectiveness to extraction has remained equivalent to the first cycle. Finally, the separation of trace elements such as lead, tin and cadmium from the raffinate of stage 2 solvent extraction is also studied by cementation with zinc powder. The study reveals that the removal of these elements and the generation of pure zinc solution can be obtained by adding 300% excess zinc powder (74 μm) at 50 °C with 500 rpm stirring speed in 60 min. The separation of copper in stage 1, nickel from copper-free aqueous solution in stage 2 and other minor elements from the raffinate of stage 2 solvent extraction ensures the proposed process is sustainable and avoids complexity in the sequential metal recovery processes.

Genetic programming for automatic skin cancer image classification

Developing a computer-aided diagnostic system for detecting various types of skin malignancies from images has attracted many researchers. However, analyzing the behaviors of algorithms is as important as developing new systems in order to establish the effectiveness of a system in real-time situations which impacts greatly how well it can assist the dermatologist in making a diagnosis. Unlike many machine learning approaches such as Artificial Neural Networks, Genetic Programming (GP) automatically evolves models with its dynamic representation and flexibility. This study aims at analyzing recently developed GP-based approaches to skin image classification. These approaches have utilized the intrinsic feature selection and feature construction ability of GP to effectively construct informative features from a variety of pre-extracted features. These features encompass local, global, texture, color and multi-scale image properties of skin images. The performance of these GP methods is assessed using two real-world skin image datasets captured from standard camera and specialized instruments, and compared with six commonly used classification algorithms as well as existing GP methods. The results reveal that these constructed features greatly help improve the performance of the machine learning classification algorithms. Unlike “black-box” algorithms like deep neural networks, GP models are interpretable, therefore, our analysis shows that these methods can help dermatologists identify prominent skin image features. Further, it can help researchers identify suitable feature extraction methods for images captured from a specific instrument. Being fast, these methods can be deployed for making a quick and effective diagnosis in actual clinic situations.

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination.

Liver glycogen is a hyperbranched glucose polymer that is involved in the maintenance of blood sugar levels in animals. The properties of glycogen are influenced by its structure. Hence, a suitable extraction method that isolates representative samples of glycogen is crucial to the study of this macromolecule. Compared to other extraction methods, a method that employs a sucrose density gradient centrifugation step can minimize molecular damage. Based on this method, a recent publication describes how the density of the sucrose solution used during centrifugation was varied (30%, 50%, 72.5%) to find the most suitable concentration to extract glycogen particles of a wide variety of sizes, limiting the loss of smaller particles. A 10 min boiling step was introduced to test its ability to denature glycogen degrading enzymes, thus preserving glycogen. The lowest sucrose concentration (30%) and the addition of the boiling step were shown to extract the most representative samples of glycogen.

Separation of Pd(II) and Zn(II) from Sulfuric Acid Solution by Commercial Extractants

Spent electroplating solutions contain a small amount of Pd(II). This Pd(II) can be concentrated by cementation with zinc metal powder. In order to recover pure palladium, a hydrometallurgical process consisting of leaching followed by solvent extraction was developed in this work. First, suitable extractants for the selective extraction of Pd(II) over Zn(II) were selected from synthetic sulfuric acid solutions. Among cationic, solvating and basic extractants employed in this work, Cyanex 301, LIX 63 and Aliquat 336 showed selectivity for Pd(II) over Zn(II) from 5 M sulfuric acid solutions. Stripping results for the loaded phases of these three extractants showed that HCl was effective at stripping Pd(II) from the loaded LIX 63 and Aliquat 336, while a mixture of inorganic acid and NaClO as an oxidizing agent was necessary to strip Pd(II) from the loaded Cyanex 301. The cemented Pd was completely dissolved using 5 M sulfuric acid solution at 25 oC, and the concentration of Pd(II) and Zn(II) in the real leaching solution was 1.926 and 0.074 g/L, respectively. Application of the optimum conditions for the selective extraction of Pd(II) over Zn(II) to the real sulfuric acid leaching solution of cemented Pd indicated that only LIX 63 and Cyanex 301 could separate Pd(II) from Zn(II). The stripping efficiency of Pd(II) by a mixture of 5 M HCl and 3% NaClO was much higher from the loaded LIX 63 than from the loaded Cyanex 301. Considering the extraction and stripping characteristics of Pd(II), LIX 63 was selected as an optimum extractant for the separation of Pd(II) from the real sulfuric acid leaching solution of cemented Pd.

A Green Method of Extracting and Recovering Flavonoids from Acanthopanax senticosus Using Deep Eutectic Solvents.

In recent years, green extraction of bioactive compounds from herbal medicines has generated widespread interest. Deep eutectic solvents (DES) have widely replaced traditional organic solvents in the extraction process. In this study, the efficiencies of eight DESs in extracting flavonoids from Acanthopanax senticosus (AS) were compared. Response surface methodology (RSM) was employed to optimize the independent variable including ultrasonic power, water content, solid-liquid ratio, extraction temperature, and extraction time. DES composed of glycerol and levulinic acid (1:1) was chosen as the most suitable extraction medium. Optimal conditions were ultrasonic power of 500 W, water content of 28%, solid-liquid ratio of 1:18 g·mL−1, extraction temperature of 55 °C, and extraction time of 73 min. The extraction yield of total flavonoids reached 23.928 ± 0.071 mg·g−1, which was 40.7% higher compared with ultrasonic-assisted ethanol extraction. Macroporous resin (D-101, HPD-600, S-8 and AB-8) was used to recover flavonoids from extracts. The AB-8 resin showed higher adsorption/desorption performance, with a recovery rate of total flavonoids of up to 71.56 ± 0.256%. In addition, DES solvent could efficiently be reused twice. In summary, ultrasonic-assisted DES combined with the macroporous resin enrichment method is exceptionally effective in recovering flavonoids from AS, and provides a promising environmentally friendly and recyclable strategy for flavonoid extraction from natural plant sources.

Chiral Pesticides with Asymmetric Sulfur: Extraction, Separation, and Determination in Different Environmental Matrices

Chiral pesticides with S atoms as asymmetric centers are gaining great importance in the search for new pesticides with new modes of action. As for the rest of the chiral pesticides, the determination of the stereoisomers separately has become crucial in the environmental risks assessment of these pesticides. Therefore, the development of suitable extraction and clean-up methods as well as efficient stereoselective analytical techniques for stereoisomers determination in environmental samples is essential. Currently, liquid/solid phase extraction, microextraction, and QuEChERS-based methods are most commonly used to obtain chiral pesticides from environmental samples. Gas, liquid, and supercritical fluid chromatography together with capillary electrophoresis techniques are the most important for the determination of the stereoisomers of chiral pesticides containing S atoms in its structure. In this study, all these techniques are briefly reviewed, and the advantages and disadvantages of each are discussed.

Analytical Evaluation of Carbamate and Organophosphate Pesticides in Human and Environmental Matrices: A Review.

Pesticides are synthetic compounds that may become environmental contaminants through their use and application. The high productivity achieved in the agricultural industry can be credited to the use and application of pesticides by way of pest and insect control. As much as pesticides have a positive impact on the agricultural industry, some disadvantages come with their application in the environment because they are intentionally toxic, and this is more towards non-target organisms. They are grouped into chlorophenols, organochlorines, synthetic pyrethroid, carbamates, and organophosphorus based on their structure. The symptoms of exposure to carbamate (CM) and organophosphates (OP) are similar, although poisoning from CM is of a shorter duration. The analytical evaluation of carbamate and organophosphate pesticides in human and environmental matrices are reviewed using suitable extraction and analytical methods.

Valorization of By-Products from Biofuel Biorefineries: Extraction and Purification of Bioactive Molecules from Post-Fermentation Corn Oil.

The aim of this work was to develop innovative and sustainable extraction, concentration, and purification technologies aimed to recover target substances from corn oil, obtained as side stream product of biomass refineries. Residues of bioactive compounds such as carotenoids, phytosterols, tocopherols, and polyphenols could be extracted from this matrix and applied as ingredients for food and feeds, nutraceuticals, pharmaceuticals, and cosmetic products. These molecules are well known for their antioxidant and antiradical capacity, besides other specific biological activities, generically involved in the prevention of chronic and degenerative diseases. The project involved the development of methods for the selective extraction of these minor components, using as suitable extraction technique solid phase extraction. All the extracted and purified fractions were evaluated by NMR spectroscopic analyses and UV–Vis spectrophotometric techniques and characterized by quali-quantitative HPLC analyses. TPC (total phenolic content) and TFC (total flavonoid content) were also determined. DPPH and ABTS radical were used to evaluate radical quenching abilities. Acetylcholinesterase (AChE), amylase, glucosidase, and tyrosinase were selected as enzymes in the enzyme inhibitory assays. The obtained results showed the presence of a complex group of interesting molecules with strong potential in market applications according to circular economy principles.

Identification and analysis of barriers for harnessing geothermal energy in India

The Indian Government envisaged generating 10 GW using geothermal power by 2030. Reaching this milestone is linked with numerous challenges, as geothermal exploitation in India is in the nascent stages. In this work, possible barrier categories and barriers to harness geothermal energy in India are identified with the help of literature review and questionnaire-based surveys. Fuzzy Delphi method is used to find the significant barriers among the listed. Subsequently, Fuzzy Analytical Hierarchy Process (Fuzzy AHP) is used to determine the relative dominance of each barrier category and the barriers within each category. Outcomes of this research show that the resource barrier category obtained highest priority. This category includes various barriers such as (i) conceptualization of geothermal reservoir, (ii) estimation of theoretical heat energy, (iii) determination of extractable power, and (iv) selection of suitable extraction schemes. Results suggest that a comprehensive conceptual model presenting the subsurface variation of thermo-hydro-geological parameters with depth at a geothermal field can support the accurate depiction of the available and extractable thermal potential. Stability of the obtained hierarchy is examined by sensitivity analysis. Findings of this study help to identify the barriers that can be reasonably encountered and to propose developmental activities to harness geothermal energy.

Speculation of feasible interaction between tri-n-octyl amine and Di-2-ethylhexylphosphoric acid from volumetric measurements

Solvent extraction serves as one of the most proven hydrometallurgical techniques for recycling and recovery of various metal values This extraction practice is based on the shuttling of the solute molecules between two non-miscible liquid phases. The affinity of the solute is motivated by the nature of the extracting agent to initiate the transference of the solute from the feed solution to the organic phase. Therefore, choice of suitable extractants plays a crucial role in solvent extraction systems. Binary extractant systems have been more effective with improved separation ability and have drawn more attention in the field of solvent extraction. The investigation of interaction between the extracting agent and diluent, two main components of the organic phase, has immense impact on the extraction efficiency. Scrutiny of physicochemical properties like molar volume, partial molar volume apparent molar volume and excess molar volume, is essential in order to obtain more insight about the synergistic extractant mixtures. The present work reports the volumetric properties of binary mixtures of a basic extractant, tri-n-octyl amine (TOA) and an acidic extractant, di-2-ethylhexylphosphoric acid (D2EHPA) at T = 298 K-318 K to predict the possible interaction existing between these two at different mole fractions. By rising the mole fraction of TOA in the mixture, the density of the binary mixture gradually decreases. Enhancement in temperature resulted an increase in molar volume of the binary mixture.

An electrocorticographic decoder for arm movement for brain–machine interface using an echo state network and Gaussian readout

Brain–machine interface (BMI) studies typically use an electrocorticogram (ECoG) to record neural signals from the surface of the cortex because of the high spatial and temporal resolution and high signal-to-noise levels of the data obtained. However, in certain medical conditions, it may not be possible to place the ECoG electrodes at the target brain regions for BMI. Consequently, developing an ECoG decoder with suitable feature extraction and selection processes is challenging. This study investigated the possibility of a novel ECoG decoder for arm movement BMI. The ECoG signals were recorded from four individuals with intractable epilepsy while imaging and performing a reach-and-grasp movement. We examined the performance of the ECoG decoder using an echo state network and 24 Gaussian readouts within the classification problem paradigm of the arm movement directions. A genetic algorithm was used to optimize the hyperparameters of the ECoG decoder. The ECoG decoder successfully classified 24 arm movement directions in the x–y, x–z, and y–z planes in execution and imagination tasks. The best hit rates were 90.9 ± 5.3 %, 92.6 ± 3.9, and 92.6 ± 4.2 for the x–y, x–z, and y–z planes, respectively. A robot arm control simulation indicated that a real-time movement BMI system could use the novel ECoG decoder. Thus, the echo state network with Gaussian readouts for classification can be a successful ECoG decoder model for motor BMIs.

Selection of a suitable viral DNA extraction method for Sheeppox virus in cell culture

A suitable method for the extraction of nucleic acids should be efficient, sensitive, rapid and simple. Moreover, ideally, good method should yield pure nucleic acid-free from any contaminant inhibitors. Several methods have been reported for viral deoxyribonucleic acid (DNA) isolation but limited information is available on quick and simple isolation of Sheeppox virus (SPPV) genomic DNA in cell culture. In this study, the healthy Vero cells and primary lamb testis cells were infected with SPPV strains such as SPPV-Jaipur, SPPV-Ranipet and SPPV-Roumanian Fanar (RF) and harvested when it exhibited clear cytopathic effect (CPE) in culture. Four different DNA extraction methods i.e., (i) Phenol/chloroform/Isoamyl alcohol method, (ii) Cell lysis buffer method, (iii) Proteinase-k method, and (iv) commercial nucleic acid extraction kit was used to extract optimum yield of viral genomic DNA from clarified culture supernatant of harvested SPPV virus. The DNA sample was characterized using the Nanodrop spectrophotometer and agarose gel electrophoresis. Significantly (p<0.05) higher yield of SPPV genomic DNA was obtained in proteinase-k method which was about 3-5 times more than other methods. Among these methods, proteinase-k protocol was found to be comparatively very effective method in terms of yield of viral genomic DNA, and was free from PCR inhibitors.

Microalgae-based bioplastics: Future solution towards mitigation of plastic wastes

Global demand for plastic materials has severely harm the environment and marine sea life. Therefore, bioplastics have emerged as an environmentally friendly alternative due to sustainability, minimal carbon footprint, less toxicity and high degradability. This review highlights the sustainable and environmentally friendly approach towards bioplastic production by utilizing microalgae as a feed source in several ways. First, the microalgae biomass obtained through the biorefinery approach can be processed into PHA under certain nutrient limitations. Additionally, microalgae biomass can act as potential filler and reinforcement towards the enhancement of bioplastic either blending with conventional bioplastic or synthetic polymer. The downstream processing of microalgae via suitable extraction and pre-treatment of bioactive compounds such as lipids and cellulose are found to be promising for the production of bioplastics. Moving on, the intermediate processing of bioplastic via lactic acid synthesized from microalgae has favoured the microwave-assisted synthesis of polylactic acid due to cost efficiency, minimum solvent usage, low energy consumption, and fast rate of reaction. Moreover, the reliability and effectiveness of microalgae-based bioplastics are further evaluated in terms of techno-economic analysis and degradation mechanism. Future improvement and recommendations are listed towards proper genetic modification of algae strains, large-scale biofilm technology, low-cost cultivation medium, and novel avocado seed-microalgae bioplastic blend.

Protein extraction from cold-pressed hempseed press cake: From laboratory to pilot scale.

During the production of industrial hempseed oil, a press cake is formed as a byproduct, which is often used as animal feed although it contains a high amount of protein that could be used for human consumption. Extracting this valuable protein would reduce food waste and increase the availability of plant-based protein. A protein extraction process based on the pH-shift method was adapted to improve the protein extraction yield from industrial hempseed press cake (HPC). Parameters such as alkali extraction pH, time, and temperature, as well as isoelectric precipitation pH, were investigated in laboratory scale and were thereafter carried out in a pilot trial to explore the suitability for future scale up. The phytic acid content of the extracted protein isolate was also analyzed to investigate any potential inhibitory effect on mineral absorption. A final protein yield of 60.6%, with a precipitated protein content of 90.3% (dw), was obtained using a constant alkali extraction pH of 10.5 for 1 h at room temperature, followed by precipitation at pH 5.5. The pilot trial showed promising results for the future production of industrial hemp protein precipitate on a larger scale, showing a protein yield of 57.0% and protein content of 90.8% (dw). The amount of phytic acid in the protein isolate produced in the optimal laboratory experiment and in the pilot trial was 0.595 and 0.557g phytic acid/100g dw, respectively, which is 83%-88% less than in the HPC. This is in the range of other plant-based protein sources (tofu, kidney beans, peas, etc.). PRACTICAL APPLICATION: Industrial hempseed press cake is a byproduct in the production of industrial hempseed oil, which is mostly used as animal feed, but has the potential to become an additional source of plant-based protein for human consumption with a suitable protein extraction method. The extracted hemp protein could be used to develop new plant-based dairy or meat analog products.