Sugarcane Bagasse Hemicellulosic Hydrolysate Research Articles

Bioethanol Production From Sugarcane Bagasse Hemicellulose Hydrolysate by Immobilized S. shehatae in a Fluidized Bed Fermenter Under Magnetic Field

Bioethanol production from sugarcane bagasse hemicellulosic hydrolysate using immobilized Scheffersomyces shehatae on magnetic biosupports in a fluidized bed bioreactor assisted by magnetic field has been studied. Fermentations were carried out in two experimental setups operating in a magnetically stabilized bed mode with transversal and axial magnetic field lines at 8 and 12 kA/m, respectively. The best results were attained when experiments were carried out using a fermenter assisted by axial field whose ethanol/substrate yield and ethanol productivity were 0.15 ± 0.8E-3 g/g and 0.055 ± 0.3E-3 g/gh. These values were 12 and 34%, respectively, higher than those observed in fermentations with transversal field lines (Tukey’s test, p < 0.05). Thus, these results are attractive and can be considered as a technological advance in the bioethanol production from biomass using this unconventional fermentation technology.

Repeated batches as a feasible industrial process for hemicellulosic ethanol production from sugarcane bagasse by using immobilized yeast cells

Currently, sugarcane bagasse (SB) is the most abundant agricultural residue generated in Brazil. Given that hemicelluloses can reach up to 30% of SB, bioconversion of this fraction into second generation ethanol (2G) is essential for the success of biorefinery based operations. For 2G ethanol production, techniques such as immobilization process could be an interesting strategy to improve process productivity and must receive special investigation. Cell immobilization of the native Brazilian pentose wild converting yeast, Scheffersomyces shehatae UFMG-HM 52.2, was proposed for the production of 2G ethanol from SB hemicellulosic hydrolysate. A 23 full experimental design was constructed with varying concentrations of sodium alginate (1.0, 1.5 and 2.0% w/v), and calcium chloride (0.1 M, 0.15 M and 0.2 M) and conditioning time (12, 18 and 24 h). Through statistical analysis, it was determined that highest ethanol yield (YP/S of 0.32 g/g) and productivity (QP of 0.146 g/L.h) were obtained with immobilization conditions of 1% sodium alginate, 0.2 M calcium chloride and 12 h of conditioning time. Repeated batches were conducted employing these defined conditions, showing the feasibility to use the system for 5 consecutive cycles. Results highlighted the use of proposed approach for ethanol production, promoting its inclusion in biorefinery operation portfolios. Hemicellulosic hydrolysate from sugarcane bagasse: a large available carbon source; S. shehatae UFMG-HM 52.2 is a Brazilian wild native pentose fermenting yeast; Immobilization conditions for this yeast were defined for ethanol production; Stability in ethanol production was demonstrated in 5 repeated consecutive batches; Our approach was demonstrated feasible for industrial 2G ethanol process development.

Polymer grade l-lactic acid production from sugarcane bagasse hemicellulosic hydrolysate using Bacillus coagulans

Lactic acid has applications in many different sectors such as food, chemicals and polymers. Its production from lignocellulosic materials has been highlighted as a possibility to overcome barriers in the production chain such as decreasing process costs and reaching better environmental performance, besides adding value to biomass. However, the needed pre-treatment that lignocellulosic material goes through to release fermentable sugars also produces inhibitor compounds for the microorganisms, such as HMF and furfural. In this work, a Bacillus coagulans strain is evaluated as an l-lactic acid producer using sugarcane bagasse hemicellulosic hydrolysate. l-Lactic acid production reached 55.99 g/L, 0.87 g/g of yield, 1.7 g/ L h −1 of productivity and 99.4% of isomeric purity. Simultaneous bio-detoxification removed 100% of HMF and furfural without any treatment prior. To the best of our knowledge, it is the first time that B. coagulans is used to lactic acid production and bio-detoxification tests.

Biosurfactants production by yeasts using sugarcane bagasse hemicellulosic hydrolysate as new sustainable alternative for lignocellulosic biorefineries

In this study, sugarcane bagasse hemicellulosic hydrolisate was evaluated as carbon source in biosurfactant production by yeasts. 27 yeasts were screened in culture media based on commercial xylose, non-detoxified and detoxified sugarcane bagasse hydrolysate to select potential strains for BS production with emulsifying and tensoactive properties. Cutaneotrichosporon mucoides UFMG-CM-Y6148 cultured in medium based on detoxified hydrolysate was the best producer of biosurfactant with a volumetric productivity of 0.167 g.L−1. h−1 and highlighted emulsifying properties in kerose as emulsification index higher than 50% and an emulsifying activity of 1.0 EU. mL−1, and its effectiveness was demonstrated over a wide range of temperature, pH and salt concentration. The chromatography and spectrometry analysis demonstrated that the BS produced by C. mucoides UFMG-CM-Y6148 was a sophorolipid. The results obtained in this study demonstrated the successful sustainable prodution of biosurfactant by yeasts in media with hemicellulosic hydrolysate and the viability of these products for biorefineries.

Candida tropicalis able to produce yeast single cell protein using sugarcane bagasse hemicellulosic hydrolysate as carbon source

The world demand for foods will be the most important bottle-neck for human being maintenance. In this context, the research for alternative proteins sources aiming human and animal feeding is a necessary approach. A wild-type strain of Candida tropicalis (KP276650) was identified as potential yeast single cell protein (YSCP) producer using sugarcane bagasse hemicellulosic hydrolysate (SBHH) as substrate. Were evaluated the cell growth and sugar consumption along fermentation assay. Biomass yield (YB, gg−1), protein percentage (%), protein yield (YP, gg−1) and yeast biomass productivity (YBP, gL−1h−1) were calculated. Fermentation assay reach a total of 96h, being the total reducing sugar present in the media (59.94gL−1) fully consumed. Final biomass produced was 16.97gL−1, and the YBP was 0.1767gL−1h−1. Percentage of protein in yeast biomass produced was 60.05%, biomass yield (YB) and protein yield (YP) were, respectively, 0.28 and 0.17gg−1. The YB and YP obtained are close to the observed for some industrial strains of yeasts. The percentage of protein is greater than the expected for most of yeasts and fungi. These results indicate that the using of C. tropicalis (KP276650) for YSCP production may to aggregate high value for sugarcane bagasse.

Production of bioethanol in sugarcane bagasse hemicellulosic hydrolysate by Scheffersomyces parashehatae, Scheffersomyces illinoinensis and Spathaspora arborariae isolated from Brazilian ecosystems.

This study aimed to evaluate new d-xylose-fermenting yeasts from Brazilian ecosystems for the production of second-generation ethanol. d-xylose-fermenting yeasts isolated from rotting wood and wood-boring insects were identified as the species Scheffersomyces parashehatae, Scheffersomyces illinoinensis, Spathaspora arborariae and Wickerhamomyces rabaulensis. Among the yeasts tested, those of Sc. parashehatae exhibited the highest ethanol production when cultivated on complex medium (Yp/set =0·437gg-1 ). Sheffersomyces illinoinensis and Sp. arborariae showed similar ethanol production in this assay (Yp/set up to 0·295gg-1 ). In contrast, in sugarcane bagasse hemicellulosic hydrolysate, Sc. parashehatae and Sc. illinoinensis exhibited similar ethanol production (Yp/set up to 0·254gg-1 ), whereas Sp. arborariae showed the lowest results (peak Yp/set =0·160gg-1 ). Wickerhamomyces rabaulensis exhibited a remarkable xylitol production (Yp/sxyl =0·681 gg-1 ), but producing low levels of ethanol (Yp/set =0·042gg-1 ). The novel d-xylose-fermenting yeasts showed promising metabolic characteristics for use in fermentation processes for second-generation ethanol production, highlighting the importance of bioprospecting research of micro-organisms for biotechnological applications. This study widens the scope for future researches that may examine the native yeasts presented, as limited studies have investigated these species previously.

Kinetic Study of the Acid Post-hydrolysis of Xylooligosaccharides from Hydrothermal Pretreatment

Hydrothermal pretreatment of sugarcane bagasse is a water-based and environment-friendly process that results in almost complete hemicellulose solubilization in oligomeric form as xylooligossacharides (XOs). However, the soluble XOs cannot be utilized by microorganisms such as yeasts, and therefore, a further break down is necessary to generate pentose (C5) monomers that can be then biotransformed into ethanol or other metabolites. The kinetics of XOs post-hydrolysis with sulfuric, maleic, and oxalic acids (the latter two being dicarboxylic acids) in a sugarcane bagasse hemicellulosic hydrolysate was assessed in a bench-scale reactor (2 L). By means of a 22 full factorial design with center point triplicate, acid mass loading and temperature were varied from 0.5 and 2.0% and from 120 to 150 °C, respectively. An irreversible first-order consecutive reaction model of the hydrolysis of XOs in liquid medium was employed. Based on an Arrhenius-type equation, a kinetic parameter estimation was performed with genetic algorithms and the Runge-Kutta methods. For the three acids, the calculated exponential factors, A 0n (n = 1, 2, and 3), ranged from 1012 to 1015 min−1; the dimensionless parameters, m n (n = 1, 2, and 3), ranged from 0.86 to 1.97; and the activation energies ranged from 89 to 129.8 kJ·mol−1. The model—developed at microscale—correctly described the observed XOs, C5, and furfural post-hydrolysis profiles in bench scale and proved the dicarboxylic acids were more selective towards post-hydrolysis by having slower kinetics than sulfuric acid.

Hemicellulosic Ethanol Production in Fluidized Bed Reactor from Sugar Cane Bagasse Hydrolysate: Interplay among Carrier Concentration and Aeration Rate

Development of an efficient consolidated process is pivotal in order to design industrially viable processes for conversion of lignocellulosic biomass into second generation (2G) ethanol. Aiming to develop process consolidation, here we explored fluidized bed reactor (FBR) for 2G ethanol production from sugar cane bagasse hemicellulosic hydrolysate (SBHH) employing calcium alginate immobilized cells of Scheffersomyces shehatae UFMG-HM 52.2. A 22-full factorial design of experiments was carried out in order to evaluate the effect of aeration rate (0.027, 0.069, and 0.111 min–1) and carrier concentration (55.55, 83.33, and 111.11 g. L–1) on the ethanol yield (YP/S) and productivity (QP). Both process variables, when used at the highest level (aeration, 0.11 min–1; immobilized carrier concentration, 111.11 g. L–1), showed maximum ethanol production (YP/S, 0.26 g/g and QP 0.17 g·L–1·h–1). Results showed the potential to use this immobilized yeast in a fluidized bed reactor for ethanol production from C5 sugar...

Microbial oil production in sugarcane bagasse hemicellulosic hydrolysate without nutrient supplementation by a Rhodosporidium toruloides adapted strain

Abstract Sugarcane bagasse hemicellulosic hydrolysate (SCBH) is a low-cost substrate for single-cell oil (SCO) production. However, this hydrolysate has an undesirable low carbon/nitrogen (C/N) ratio and contains inhibitors. Yeast adaptation is a simple strategy to overcome the presence of inhibitors, while the concentration of the hydrolysate and glycerol supplementation are alternatives to solve the low C/N limitation. This work aimed to investigate the adaptation of the yeast Rhodosporidium toruloides in undetoxified SCBH and its use in SCO production. The adapted strain produced more lipids than the parental strain: the concentration of SCBH or the addition of glycerol increased the lipid content, concentration, and productivity to at least 108%, 175%, and 118%, respectively. Lipid production in SCBH was done without salts or nitrogen supplementation. Transcriptomic study showed that hydrolysate-tolerance- and lipid accumulation-related genes were strongly induced. These genes can be targets for metabolic engineering.

Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta).

Yeasts are an important component of insect gut microbial content, playing roles such as degradation of polymers and toxic compounds, biological control, and hormone, vitamin, and digestive enzyme production. The xylophagous beetle gut is a hyperdiverse habitat and a potential source of new species with industrial abilities such as enzyme production, pentose fermentation, and biodetoxification. In this work, samples of Veturius transversus (Passalidae, Coleoptera, and Insecta) were collected from the Central Amazon Rainforest. Their guts were dissected and a total of 20 microbial colonies were isolated using sugarcane bagasse hemicellulosic hydrolysate. They were identified as having 10 distinct biochemical profiles, and genetic analysis allowed identification as three clades in the genera Candida, Williopsis, and Geotrichum. All colonies were able to assimilate D-xylose and 18 were able to produce xylitol, especially a strain of Geotrichum, with a maximum yield of 0.502 g·g−1. These results agree with a previous prediction that the microbial community associated with xylophagous insects is a promising source of species of biotechnological interest.

Xylitol production and furfural consumption by a wild type Geotrichum sp.

Xylitol is a five carbons polyol with promising medical applications. It can be obtained from chemical d-xylose reduction or by microbial fermentation of Sugarcane Bagasse Hemicellulosic Hydrolysate. For this last process, some microbial inhibitors, as furfural, constitute severe bottleneck. In this case, the use of strains able to produce xylitol simultaneously to furfural neutralization is an interesting alternative. A wild-type strain of Geotrichum sp. was detected with this ability, and its performance in xylitol production and furfural consumption was evaluated. Furthermore, were analyzed its degradation products. Geotrichum sp. produced xylitol from d-xylose fermentation with a yield of 0.44 g·g-1. Furfural was fully consumed in fermentation assay and when provided in the medium until concentration of 6 g·L-1. The furfural degradation product is not an identified molecule, presenting a molecular weight of 161 g·mol-1, an uncommon feature for the microbial metabolism of this product. This strain presents most remarkable potential in performing furfural consumption simultaneous to xylitol production. Subsequent efforts must be employed to establish bioprocess to simultaneous detoxification and xylitol production by Geotrichum sp.

Production of d-lactate from sugarcane bagasse and corn stover hydrolysates using metabolic engineered Escherichia coli strains

In this study, the lactogenic Escherichia coli strain JU15 was used and modified to produce d-lactate (d-LA) from plant hydrolysates with a minimal nutrient addition in pH controlled fermenters. Results showed that strain JU15 produces d-LA with high yield and productivity in laboratory simulated hydrolysate media and actual sugar cane bagasse hemicellulosic hydrolysate. Strain JU15 showed sequential carbon source utilization and acetic acid production. The l-lactic and acetic acid production pathways were deleted in JU15, resulting strain AV03 (JU15 ΔpoxB, ΔackA-pta, ΔmgsA), which showed simultaneous consumption of glucose and xylose and no acetic acid production in the simulated hydrolysate. The d-LA yield from hydrolysate sugars was close to 0.95gD-LA/gsugars in all cases. Our results show that d-LA can be produced from plant hydrolysates in simple batch fermentation processes with a high productivity using engineered E. coli strains at fermenter scales from 0.2 up to 10L.

Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method

Abstract The aim of this study is to determine the optimum condition for biohydrogen production from sugarcane bagasse (SCB) hydrolysate using a central composite design and response surface methodology (RSM). SCB was hydrolyzed with 0.5% (v/v) sulfuric acid at 121 °C, 0.15 MPa for 60 min in an autoclave at a solid to liquid ratio of 1:15 (g:mL). Heat-treated bacterium obtained from a hydrogen producing fermentor was used as the inoculum. The interaction of three factors, i.e., substrate concentration, substrate:buffer ratio and inoculum:substrate ratio on hydrogen production potential (P) were investigated. The results indicated that the substrate concentration, substrate:buffer ratio and inoculum:substrate ratio had a significant influence effect on P. An optimal condition was found at substrate concentration of 22.77 g-total sugar L−1, 4.31 substrate:buffer ratio, and 0.31 inoculum:substrate ratio resulted in a maximum P of 6980 mL H2 L−1. The confirmation experiment results indicated that optimum P was statistically significant, from the predicted value obtained by RSM which suggests that RSM could be efficiently used to optimize a biohydrogen production from SCB hydrolysate using mixed cultures. These results indicates that the SCB hemicellulose hydrolysate is suitable as a fermentation media for producing biohydrogen. This approach will add value to SCB by converting agricultural waste into a safe and clean form of energy.

Effect of volumetric oxygen transfer coefficient (kLa) on ethanol production performance by Scheffersomyces stipitis on hemicellulosic sugarcane bagasse hydrolysate

Experimental evaluation of the effect of the agitation speed and aeration rate (measured by kLa) and energy required for ethanol production using sugarcane bagasse hemicellulosic hydrolysate (SBHH) by Scheffersomyces stipitis were studied. Fermentation and purification stages were simulated using the software Aspen Plus with experimental data to understand the overall energy performance of the process. In all experiments, fermentative parameters and the thermal energy required in the ethanol production process were strongly influenced by kLa values. The optimum initial kLa to achieve the maximal ethanol concentration (15.03gL−1) and the minimal thermal energy required (1.85×105KW per kg ethanol), were found at 8.0h−1 (450rpm and 0.6vvm). Under this condition, the ethanol yield and productivity were 0.37gg−1 and 0.30gL−1h−1, respectively. The current study highlights the ethanol production improvement from hemicellulose hydrolysate by S. stipitis and will contribute to developing a more efficient strategies for fermentation of both cellulose and hemicellulose hydrolysates.

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

Many toxic compounds are produced and released in the hemicellulosic hydrolyzates during the acid pretreatment step, which are required for the disruption of the lignocelluloses matrix and sugars release. The conventional methods of detoxification i.e. overliming, activated charcoal, ion exchange or even membrane-based separations have the limitations in removal of these toxic inhibitors in fermentation process. Hence, it is imperative to explore biological methods to overcome the inhibitors by minimizing the filtration steps, sugar loss and chemical additions. In the present study we screened sixty-four strains of yeasts to select potential strains for detoxification of furfural, acetic acid, ferulic acid, 5-hydroxymethyl furfural (5-HMF) as carbon and energy source. Among these strains Pichia occidentalis M1, Y1'a, Y1'b and Y3' showed a significant decrease in the toxic compounds but we selected two best yeast strains i.e. P. occidentalis Y1'a and P. occidentalis M1 for the further experiments with an aim to remove the fermentation inhibitors. The yeasts P. occidentalis Y1'a and P. occidentalis M1 were grown aerobically in sugarcane bagasse hemicellulose hydrolysate under submerged cultivation. For each yeast, a 2(2) full factorial design was performed considering the variables-pH (4.0 or 5.0) and agitation rate (100 or 300rpm), and the percentage removal of HMF, furfural, acetic acid and phenols from hemicellulosic hydrolysates were responsive variables. After 96h of biological treatment, P. occidentalis M1 and P. occidentalis Y1'a showed 42.89 and 46.04% cumulative removal of inhibitors, respectively.

Xylitol production by yeasts isolated from rotting wood in the Galápagos Islands, Ecuador, and description of Cyberlindnera galapagoensis f.a., sp. nov.

This study evaluated D-xylose-assimilating yeasts that are associated with rotting wood from the Galápagos Archipelago, Ecuador, for xylitol production from hemicellulose hydrolysates. A total of 140 yeast strains were isolated. Yeasts related to the clades Yamadazyma, Kazachstania, Kurtzmaniella, Lodderomyces, Metschnikowia and Saturnispora were predominant. In culture assays using sugarcane bagasse hemicellulose hydrolysate, Candida tropicalis CLQCA-24SC-125 showed the highest xylitol production, yield and productivity (27.1 g L(-1) xylitol, Y p/s (xyl) = 0.67 g g(-1), Qp = 0.38 g L(-1). A new species of Cyberlindnera, strain CLQCA-24SC-025, was responsible for the second highest xylitol production (24 g L(-1), Y p/s (xyl) = 0.64 g g(-1), Qp = 0.33 g L(-1) h(-1)) on sugarcane hydrolysate. The new xylitol-producing species Cyberlindnera galapagoensis f.a., sp. nov., is proposed to accommodate the strain CLQCA-24SC-025(T) (=UFMG-CM-Y517(T); CBS 13997(T)). The MycoBank number is MB 812171.

New cultive medium for bioconversion of C5 fraction from sugarcane bagasse using rice bran extract.

The use of hemicellulosic hydrolysates in bioprocesses requires supplementation as to ensure the best fermentative performance of microorganisms. However, in light of conflicting data in the literature, it is necessary to establish an inexpensive and applicable medium for the development of bioprocesses. This paper evaluates the fermentative performance of Scheffersomyces (Pichia) stipitis and Candida guilliermondii growth in sugarcane bagasse hemicellulosic hydrolysate supplemented with different nitrogen sources including rice bran extract, an important by-product of agroindustry and source of vitamins and amino acids. Experiments were carried out with hydrolysate supplemented with rice bran extract and (NH₄)₂SO₄; peptone and yeast extract; (NH₄)₂SO₄, peptone and yeast extract and non-supplemented hydrolysate as a control. S. stipitis produced only ethanol, while C. guilliermondii produced xylitol as the main product and ethanol as by-product. Maximum ethanol production by S. stipitis was observed when sugarcane bagasse hemicellulosic hydrolysate was supplemented with (NH₄)₂SO₄, peptone and yeast extract. Differently, the maximum xylitol formation by C. guilliermondii was obtained by employing hydrolysate supplemented with (NH₄)₂SO₄ and rice bran extract. Together, these findings indicate that: a) for both yeasts (NH₄)₂SO₄ was required as an inorganic nitrogen source to supplement sugarcane bagasse hydrolysate; b) for S. stipitis, sugarcane hemicellulosic hydrolysate must be supplemented with peptone and yeast extract as organic nitrogen source; and: c) for C. guilliermondii, it must be supplemented with rice bran extract. The present study designed a fermentation medium employing hemicellulosic hydrolysate and provides a basis for studies about value-added products as ethanol and xylitol from lignocellulosic materials.

Continuous System of Combined Columns of Ion Exchange Resins and Activated Charcoal as a New Approach for the Removal of Toxics from Sugar Cane Bagasse Hemicellulosic Hydrolysate

A continuous system of combined columns of ion exchange resins and activated charcoal was proposed as a new approach for the removal of toxics from sugar cane bagasse hemicellulosic hydrolysate. A factorial design was carried out to evaluate the influence of temperature and feed flow rate in the performance of the detoxification procedure. By using a temperature of 30 °C and a flow rate of 2.5 VB/h, the total removal of furfural and 5-(hydroxymethyl) furfural was observed, as well as a large reduction in the color and concentration of phenolic compounds. Removal of metals was also observed, with 50, 63, and 23% reduction in the concentration of copper, chromium, and nickel, respectively. The product of the detoxification process was a hydrolysate with a reduced concentration of inhibitors that can be used as a raw material for a number of processes to obtain products of economic and social interest.

Bioethanol Production from Sugarcane Bagasse by a Novel Brazilian Pentose Fermenting YeastScheffersomyces shehataeUFMG-HM 52.2: Evaluation of Fermentation Medium

Bioconversion of hemicellulosic sugars into second generation (2G) ethanol plays a pivotal role in the overall success of biorefineries. In this study, ethanol production performance of a novel xylose-fermenting yeast,Scheffersomyces shehataeUFMG-HM 52.2, was evaluated under batch fermentation conditions using sugarcane bagasse (SB) hemicellulosic hydrolysate as carbon source. Dilute acid hydrolysis of SB was performed to obtain sugarcane bagasse hemicellulosic hydrolysate (SBHH). It was concentrated, detoxified, and supplemented with nutrients in different formulations to prepare the fermentation medium to the yeast evaluation performance.S. shehataeUFMG-HM 52.2 (isolated from Brazilian Atlantic rain forest ecosystem) was used in fermentations carried out in Erlenmeyer flasks maintained in a rotator shaker at 30°C and 200 rpm for 72 h. The use of a fermentation medium composed of SBHH supplemented with 5 g/L ammonium sulfate, 3 g/L yeast extract, and 3 g/L malt extract resulted in 0.38 g/g of ethanol yield and 0.19 g L.h of volumetric productivity after 48 h of incubation time.

Evaluation of Rice Bran Extract as a Nitrogen Source for Improved Hemicellulosic Ethanol Production from Sugarcane Bagasse by New Xylose-Fermenting Yeast Strains Isolated from Brazilian Forests

Xylose is the main sugar in hemicellulosic hydrolysates and its fermentation into ethanol by microorganisms is influenced by nutritional factors, such as nitrogen source, vitamins and other elements. Rice bran extract (RBE) is an inexpensive nitrogen source primarily consisting of high amount of protein. This study evaluates the potential of RBE as a nitrogen source for the “hemicellulosic ethanol” production from sugarcane bagasse dilute acid hydrolysate by novel yeast strains Scheffersomyces shehatae (syn. Candida shehatae) CG8-8BY and Spathaspora arborariae UFMG-HM19.1A, isolated from Brazilian forests. Two different media formulations were used for inoculum preparation and production medium, using yeast extract and RBE as nitrogen sources. S. shehatae CG8-8BY showed ethanol production of 17.0 g/l with the ethanol yield (0.33 g/g) and fermentation efficiency (64 %) from medium supplemented with RBE. On the other hand, S. arborariae presented 5.4 g/l of ethanol production with ethanol yield (0.14 g/g) and fermentation efficiency (21 %) in a fermentation medium supplemented with RBE. Appropriate media formulation is an important parameter to increase the productivity of bioconversion process and RBE proved to be an efficient and inexpensive nitrogen source to supplement sugarcane bagasse hemicellulosic hydrolysate for second generation ethanol production.