Fruit Sugar Content Research Articles

Sucrose phononic crystal-based sensor via acoustically induced transparency

Abstract Accurate detection of sucrose concentration is vital for assessing sugar content in fruits, vegetables, and nuts. This article introduces an innovative method for sensing various sucrose concentrations using an acoustically induced transparency system integrated by a solid mechanic phononic crystal framework. The detector architecture is based on a two-dimensional phononic crystal that employs poly methyl methacrylate as its foundational material, complemented by a regularly spaced array of circular tungsten pillars. The layout features a linear waveguide linked to two coupled ring resonators, with the upper resonator housing four columns filled with varying sucrose concentrations, thereby enabling resonance frequencies that are specifically tailored to each concentration. Simulation findings demonstrate a strong correlation between the shifts in transmission peak frequency and the associated sound velocities and material densities. The sensor’s functionality is further confirmed through coupled mode theory, representative an excellent alignment with the sensor’s presentation. Particularly, the sensor achieves an impressive average quality factor of 53515, an average sensitivity of 195 Hz, a figure of merit of 4.75, a signal-to-noise ratio of 1.25, a resolution of 40 Hz, and average insertion losses of 1.48 dB. These results underscore the acoustically induced transparency system’s potential for precise sucrose concentration sensing, offering remarkable sensitivity and accuracy for agricultural applications.

CBL1/CIPK23 phosphorylates tonoplast sugar transporter TST2 to enhance sugar accumulation in sweet orange (Citrus sinensis).

Fruit taste quality is greatly influenced by the content of soluble sugars, which are predominantly stored in the vacuolar lumen. However, the accumulation and regulation mechanisms of sugars in most fruits remain unclear. Recently, we established the citrus fruit vacuole proteome and discovered the major transporters localized in the vacuole membrane. Here, we demonstrated that the expression of tonoplast sugar transporter 2 (CsTST2) is closely associated with sugar accumulation during sweet orange (Citrus sinensis) ripening. It was further demonstrated that CsTST2 had the function of transporting hexose and sucrose into the vacuole. Overexpression of CsTST2 resulted in an elevation of sugar content in citrus juice sac, calli, and tomato fruit, whereas the downregulation of its expression led to the reduction in sugar levels. CsTST2 was identified as interacting with CsCIPK23, which binds to the upstream calcium signal sensor protein CsCBL1. The phosphorylation of the three serine residues (Ser277, Ser337, and Ser354) in the loop region of CsTST2 by CsCIPK23 is crucial for maintaining the sugar transport activity of CsTST2. Additionally, the expression of CsCIPK23 is positively correlated with sugar content. Genetic evidence further confirmed that calcium and CsCIPK23-mediated increase in sugar accumulation depends on CsTST2 and its phosphorylation level. These findings not only unveil the functional mechanism of CsTST2 in sugar accumulation, but also explore a vital calcium signal regulation module of CsCBL1/CIPK23 for citrus sweetness quality.

Exogenous GABA improves tomato fruit quality by contributing to regulation of the metabolism of amino acids, organic acids and sugars

To better explore the impact of exogenous GABA on tomato fruit quality, we explored the effects of GABA (20 mM) on tomato fruits at different developmental stages. The results showed that spraying GABA 3 days before harvest improved the quality of fruits, primarily in terms of amino acid, organic acid, and soluble sugar content. Transcriptome and metabolome data indicated that exogenous GABA enters cells and mitochondria through GABA transporters, thereby simulating endogenous GABA metabolism and enhancing the activity of genes related to amino acids and organic acids metabolism, and elevating the levels of amino acids and organic acids. Moreover, the application of exogenous GABA promoted the contents of glucose, fructose, and sucrose by affecting the expression of genes related to sugar metabolism. It was found that the mechanism of exogenous GABA in increasing fruit sugar content involved promoting sucrose synthesis, partially inhibiting glycolysis, and increasing the content of trehalose 6-phosphate. This study also provides a new model for the mechanism through which exogenous GABA affects tomato quality.

Gene expression and soluble sugar accumulation in perennial fruits

Abstract Soluble sugars primarily contribute to fruit flavouring and are crucial for fruit quality. Carbohydrate accumulation in fruits is a multifaceted process that can be influenced by their biosynthesis capacity, sink strength, homeostasis, and vacuolar storage ability. Moreover, total soluble sugar contents in fruits also vary among different varieties/species and environmental conditions. Numerous studies have elucidated the carbohydrate regulatory mechanism in fruits; however, there is a knowledge gap regarding the most important contributor to soluble sugar accumulation in perennial fruits. Here, we briefly discussed the recent advances, including carbohydrate long-distance transportation, metabolism and vacuolar storage, and transcript factors for soluble sugar accumulation in fruits. Most importantly, we elucidated that the sink strength in fruit is the most crucial factor that can trap more soluble sugars by maintaining continuous unloading to fruit by genes of cell wall invertase; moreover, genes of sucrose transporters/pyrophosphatase play key roles in vacuolar storage of soluble sugars in perennial fruits. This review delivers a comprehensive summary of recent findings for soluble sugar regulation and fruit quality improvement, which may facilitate sugar optimisation in fruit crops for enhanced fruit quality.

Releasing a sugar brake generates sweeter tomato without yield penalty

In tomato, sugar content is highly correlated with consumer preferences, with most consumers preferring sweeter fruit1–4. However, the sugar content of commercial varieties is generally low, as it is inversely correlated with fruit size, and growers prioritize yield over flavour quality5–7. Here we identified two genes, tomato (Solanum lycopersicum) calcium-dependent protein kinase 27 (SlCDPK27; also known as SlCPK27) and its paralogue SlCDPK26, that control fruit sugar content. They act as sugar brakes by phosphorylating a sucrose synthase, which promotes degradation of the sucrose synthase. Gene-edited SlCDPK27 and SlCDPK26 knockouts increased glucose and fructose contents by up to 30%, enhancing perceived sweetness without fruit weight or yield penalty. Although there are fewer, lighter seeds in the mutants, they exhibit normal germination. Together, these findings provide insight into the regulatory mechanisms controlling fruit sugar accumulation in tomato and offer opportunities to increase sugar content in large-fruited cultivars without sacrificing size and yield.

Genetic and QTL analysis of sugars and acids content in sweet cherry (Prunus avium L.)

Abstract Sweet cherry is very appreciated by consumers because of its attractive appearance and taste, which is determined by the balanced sweet-sour flavor. In this work, the genetics of soluble solid content (SSC), titratable acidity (TA), sugars and organic acids was investigated in sweet cherry to facilitate breeding improvement for fruit quality. The fruits of five sweet cherry populations (N = 372), three F1 and two F2, were sampled over two years to evaluate SSC, TA, and the content of individual sugars (glucose, fructose, sorbitol, and sucrose) and organic acids (malic, quinic, oxalic, citric and shikimic) by ultra-performance liquid chromatography (UPLC). Glucose, followed by fructose, was the most abundant sugar, while malic acid was the predominant acid. Sorbitol and malic acid were the most stable compounds between years, and had the highest heritability, being also the best correlated to SSC and TA respectively, revealing their relevance for breeding. Significantly positive correlations were observed among sugars and SSC, and acids and TA, but high interannual variability between years was observed for all traits. QTL mapping for SSC, sugars, TA, and organic acids was performed using a multi-family approach with FlexQTL™. Twenty QTLs were detected consistently during the two phenotyped years, and several relevant regions with overlapping QTLs for sugars and acids were also identified. The results confirmed major stable SSC and TA QTLs on the linkage groups 4 and 6, respectively. Within the main LG4 SSC QTL region, where maturity and fruit development time QTLs have been previously detected, three stable sugar (glucose, sorbitol, and sucrose) and two acid (quinic, shikimic) QTLs were also identified, suggesting a pleiotropic effect of ripening date on the content of these compounds. The major malic acid QTL overlapped with TA QTL on LG6, thus TA QTL mapping in LG6 may correspond to malic acid QTLs. Haplotype analyses of major SSC and sugars QTL in LG4, and TA and malic acid in LG6 revealed haplotypes of breeding interest. Several candidate genes previously identified in other Prunus fruit species, like peach, were found to collocate with the QTLs detected herein. This work reports QTLs regions and haplotypes of sugar and acid content in a Prunus non-climacteric stone fruit for the first time.

Comparative Evaluation of Calcium Sources and their Mode of Use on Apple Production in Acidic Soil under High Density Plantation System

Apples grown in Kashmir province are extremely prone to deterioration, particularly in post-harvest storage. Calcium is amongst the vital mineral element deceiving apple fruit quality, predominantly in acid soils deficit in available calcium. Calcium in sufficient quantity helps to retain apple fruit firmness and reduces the occurrence of physiological disorders, it also plays essential role to improve yield and quality of produce. Modes of calcium application and its different doses on two apple varieties was tested to improve yield and quality at Ambri Apple Research Centre, Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, India. The direct application of calcium to apple as spray is most effective method for escalating fruit calcium content and quality. Among sources and their mode of application calcium nitrate foliar application performed better than foliar application of calcium chloride in improving fruit quality and yield of Golden Delicious than Red Chief Camspur, whereas soil application of calcium nitrate showed least response to improve fruit quality and yield in both varieties under acidic soil atmosphere. Physiological loss in weight at 30 days and 60 days after harvesting was lower in foliar applied calcium than soil calcium application. The significant variation in total sugar content and TSS of apple fruits were observed in different calcium sources and their mode of application. Calcium nitrate foliar spray performed better than calcium chloride foliar spray in improving fruit quality. Our study did not find any adversity of dosage in plants whether applied to soil or directly to plant and we did observe better yields and quality at higher calcium doses. Among varieties Golden Delicious performed better than Red Chief Camspur. Results of research evidenced that preharvest calcium sprays increase yield, improved fruit quality and reduced its deterioration in post-harvest storage.

Potential reformulation of food products two years after implementation of Nutri-Score in Belgium

Abstract Background Nutri-score is a front of pack labeling system that provides consumers with simplified information of the nutritional quality of food products. It is based on a five color (green to red) or letter (A to E) scheme that grades the quality of the product using an algorithm that takes into account the energy, sugar, saturated fats, salt, fiber, protein content, and percent of fruits and vegetables. In Belgium, Nutri-score was implemented on a voluntary basis in April 2019. This study aims to explore reformulation of products with Nutri-score by comparing their nutrient content and Nutri-score distribution in 2019 and 2021. Methods Product data from one of the major retailers was used in the analysis. Similar products in 2019 and 2021 with matched barcodes were used. Descriptive statistics were computed and statistical analyses such as the signed rank test and the McNemars test were performed to respectively evaluate the changes in the nutrient content and Nutri-score. The tests were conducted at 5% level of significance. Results From the matched sample of 668 products, about 55% had a Nutri-score of A and B in both 2019 and 2021. The proportion of food products with Nutri-Score of A/B and of D/E was not significantly different after two years of Nutri-Score implementation. Results showed a significant decrease in the median energy content of dairy, sauces, and meat products and in the median sugar content of dairy and fruit and vegetable products. There was a significant increase in the median fiber content of the dairy, meat products, and non-alcoholic beverages. For all food groups, the salt content of the products in 2019 and 2021 was not significantly different. Conclusions This two-year interval analysis described the changes in the classification and reformulation of the products with Nutri-score. Favorable changes in the nutrient quality of the product profiles reveals improvement in their reformulation. Key messages • In this analysis, changes has been observed in the nutrient content of food products between 2019 and 2021. • Producers or manufacturers should continue working on commitments to enhance the nutritional quality of the products delivered to consumers.

Effect of fruit pulp and sugar concentration on the physicochemical, phytochemical and sensory characteristics of star fruit (Averrhoa carambola) leather

Starfruit is an underutilized fruit and is rich in vitamin C. Fruit leather from starfruit was prepared in this study and analyses of its physicochemical, phytochemical, and sensory characteristics were performed. Five samples named A, B, C, D and E were prepared with different proportions of fruit pulp:sugar of 70:30, 75:25, 80:20, 85:15 and 90:10, respectively. All the prepared samples were subjected to analyses. Phytochemical contents were found to increase with increasing fruit pulp proportion, thus sample E (90:10) was found to be the best in terms of phytochemical content. Similarly, product sample C (80:20) was scored the highest by the panelist in terms of sensory aspect. The best product sample C had total soluble solids (TSS) of 31 ± 2 °Bx, total ash content of 2.482 ± 0.001 %, acid insoluble ash of 1.0013 ± 0.002 %, crude protein of 0.5902 ± 0.004 %, crude fat of 0.3514 ± 0.003 %, crude fiber of 0.8991 ± 0.003 %, carbohydrate of 95.67 ± 0.04 %, vitamin C of 24.24 ± 0.1 mg/100g, total energy of 338.11 ± 0.04 Kcal/100 g, total phenolic content (TPC) of 6.105 ± 0.001 mg GAE/g of dry extract, total flavonoid content (TFC) of 1.125 ± 0.002 mg QE/g of dry extract and DPPH scavenging activity of 27.268 ± 0.001 %. Panelists responded favorably to the prepared product samples.

Identification of Key Genes Controlling Sugar and Organic Acid Accumulation in Wampee Fruit (Clausena lansium) via Genome Assembly and Genome-wide Association Analysis.

Wampee (Clausena lansium) is an economically significant subtropical fruit tree widely cultivated in Southern China. To provide high-quality genomic resources for C. lansium, we report a chromosome-level genome sequence for the "JinFeng" cultivar. The 297.1 Mb C. lansium genome contained nine chromosomes with a scaffold N50 of 29.2 Mb and encoded 23,468 protein-coding genes. Selective sweep analysis between sweet and sour C. lansium varieties and genome-wide association analysis identified 14 candidate genes putatively involved in sugar and acid accumulation. ClERF061, encoding an ethylene response factor, and ClSWEET7, encoding a Sugars Will Eventually be Exported Transporters (SWEET) family protein, were proposed as key regulators of the sweet and sour tastes of the wampee fruit. ClERF061 and ClSWEET7 overexpression in tomatoes increased the total sugar and acid content in fruits. ClSWEET7 promoter activation by ClERF061 was confirmed via Nicotiana benthamiana transient expression. Our study provides valuable genomic resources for C. lansium genetics and breeding.

Sink Strength Dynamics Based on Potential Growth and Carbohydrate Accumulation in Strawberry Fruit

Fruit size and sugar content are key determinants of fruit quality, influenced by environmental factors and agronomic practices and sink strength provided by the genetic potential. Strawberry (Fragaria ×ananassa) produces fruits arranged in inflorescences, whose growth is affected by carbon competition between them. The competitive ability is termed as sink strength, which can be quantified as the potential growth rate under sufficient resource supply and/or no carbon competition among sinks, referred to as non-limiting conditions. Most previous studies did not observe potential growth, thereby failing to adequately evaluate sink strength and to assess the influence of environmental factors and agronomic practices on fruit growth. This study aimed to investigate the potential growth of strawberry fruits and analyze its sink strength dynamics. Non-limiting conditions were established by removing flowers to one fruit per inflorescence in a greenhouse experiment with plants grown in soil and given water and nutrients through drip irrigation. Fruits were harvested every 5 days from 5 to 55 days after anthesis (DAA), measuring the size, weight, and concentrations of major soluble carbohydrates in strawberry (sucrose, glucose, and fructose) and starch. Sink strength was represented by absolute growth rate based on fruit weight, and its components, sink size and sink activity, were represented by weight and relative growth rate, respectively. Fruit volume and weight showed a gradual linear increase at 5 DAA and then rapidly increased, following a single sigmoid curve between 30 and 55 DAA. Fruits primarily accumulated glucose and fructose during early growth, shifting to sucrose after 35 DAA. Starch concentration peaked at 5 DAA and then exponentially decreased. Sink strength exhibited a single peak between 40 DAA and 45 DAA. Sink strength gradually increased with sink size until 30 DAA, whereas sink activity significantly decreased until 30 DAA. Thereafter, sink strength and sink activity exhibited a peak, whereas sink size continued to increase. These results suggest that the major determinant of sink strength was sink size during early fruit growth, shifting to sink activity during late growth.

Exploring Italian Autochthonous Punica granatum L. Accessions: Pomological, Physicochemical, and Aromatic Investigations.

Autochthonous Italian pomegranate accessions are still underexplored, although they could be an important resource for fresh consumption, processing, and nutraceutical uses. Therefore, it is necessary to characterize the local germplasm to identify genotypes with desirable traits. In this study, six old Italian pomegranate landraces and a commercial cultivar (Dente di Cavallo) were investigated, evaluating their fruit pomological parameters, physicochemical (TSS, pH, TA, and color) characteristics, sugar content, and aromatic profiles (HeadSpace Solid-Phase MicroExtraction (HS-SPME)) coupled with Gas Chromatographyass Spectrometry (GC-MS) of pomegranate juices. Significant differences were observed in the size and weight of the seed and fruits (127.50-525.1 g), as well as the sugar content (100-133.6 gL-1), the sweetness (12.9-17.6 °Brix), and the aroma profiles. Over 56 volatile compounds, predominantly alcohols (56%), aldehydes (24%), and terpenes (9%), were simultaneously quantified. Large variability among the genotypes was also statistically confirmed. The results indicate a strong potential for commercial exploitation of this germplasm, both as fresh and processed fruit, and highlight its versatility for diverse applications. The genetic diversity of the autochthonous pomegranate accessions represents a precious heritage to be preserved and enhanced. This work represents a preliminary step toward a more comprehensive characterization and qualitative valorization of the Italian pomegranate germplasm.

Microbial biofertilizers and algae-based biostimulant affect fruit yield characteristics of organic processing tomato.

Microbial biofertilizers and algae-based biostimulants have been recognized for supporting sustainable agriculture. Field experiments were conducted in 2022 and 2023 growing seasons in an organic farm located in Ferrara (Italy) with the aim of evaluating plant growth-promoting microorganisms (PGPMs) and algae-based biostimulants (Biost) in tomato (Solanum lycopersicum L.). The experimental treatments were: (i) two microbial biofertilizers (PGPM_1, PGPM_2) and no inoculated plants (No_PGPM); and (ii) two algae-based biostimulant rates (0.5% (Biost_0.5%), 1.0% (Biost_1.0%)) and no application (No_Biost). PGPMs were applied at transplanting, while biostimulants at 15 and 30 days after transplanting. Treatments were replicated three times according to a split-plot experimental design. Plant characteristics were evaluated at 30 days after transplanting in No_Biost treatments. During tomato cultivation, soil plant analysis development (SPAD), nitrogen difference vegetation index (NDVI), leaf area index (LAI) and photosynthetic photon flux density (PPFD) were monitored. Tomato yield was determined. PGPM_2 showed the highest shoot biomass (132.9 g plant-1), plant height (44.7 cm), leaf number (34.0 plant-1) and root biomass (9.22 g plant-1). Intermediate values were observed in PGPM_1, while all parameters were lower in No_PGPM. Both PGPMs achieved higher values of SPAD, NDVI, PPFD and LAI than No_PGPM. Biost_1.0% increased all measured growth parameters followed by Biost_0.5% and No_Biost, respectively. Tomato yield was the highest for PGPM_2-Biost_1.0% (67.2 t ha-1). PGPMs affected fruit size and sugar content, while biostimulants were associated with color and lycopene. The application of microbial biofertilizers and algae-based biostimulants could be part of environment-friendly practice in organic farming. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

How Nano-ZnO Affect Tomato Fruits (Solanum lycopersicum L.)? Analysis of Selected Fruit Parameters.

Tomato (Solanum lycopersicum L.), as one of the most valuable horticulture crops, was chosen to investigate the effect of nanoparticles (NPs) in the form of nano-ZnO combined with conventional fertilizer on the quality of tomato fruits, including their antioxidant potential (total antioxidant activity, lycopene and β-carotene content), sugars content and allergenic potential (profilin and Bet v 1 content). Nano-ZnO was implemented during plant cultivation, applied by foliar spraying or directly via soil, at three different concentrations (50, 150 and 250 mg/L). The obtained results suggest that the usage of NPs during tomato plant cultivation had minor impacts on parameters such as total antioxidant activity or the content of selected allergens. Even though the total antioxidant activity was not affected by nano-ZnO, the malondialdehyde activity (MDA) content was notably decreased in fruits under nano-ZnO treatment. The content of lycopene and β-carotene was significantly affected by the use of nano-ZnO. Moreover, the usage of nano-ZnO significantly increased the total sugar content in fruits treated with nanoparticles via foliar spraying. Based on the obtained results, it can be stated that nano-ZnO, regardless of the method of application, significantly affected tomato fruits which can be beneficial for fruit production.

Physiological and Transcriptomic Analysis of Citrus Fruit Segment Drying under Facility-Forcing Cultivation

Facility-forcing cultivation could effectively improve the quality of ‘Beni Madonna’ citrus (Citrus nankao × C. amakusa) and advance the ripening period. However, segment drying (KS) before harvest caused fruit quality deterioration and commodity value loss. In this research, we investigated the physiological and molecular characteristics involved in citrus segment drying under facility-forcing cultivation. The juice yield, sugar, acid, vitamin C, and lignin contents in KS fruits were significantly decreased, and the contents of pectin and cellulose were significantly increased. The relative contents of abscisic acid and abscisic acid glucosyl ester in KS fruits were significantly decreased. A total of 1215 differentially expressed genes (DEGs) were screened by transcriptome sequencing. DEGs were significantly enriched in water metabolism, sugar metabolism, transportation, cell wall and phenylpropanoid biosynthesis, and plant hormone signal transduction. The decrease in water absorption and sugar synthesis, the increase of pectin and cellulose synthesis, and the decrease in ABA accumulation may be the main reasons that cause citrus fruit segments to dry under facility-forcing cultivation.

ALA Promotes Sucrose Accumulation in Early Peach Fruit by Regulating SPS Activity.

5-Aminolevulinic acid (ALA), as a novel plant growth regulator, is a critical precursor for the biosynthesis of porphyrin compounds in all organisms. Many studies have reported that exogenous ALA treatment could improve fruit sweetness. However, the mechanism by which ALA promotes the increase in sugar content in fruit remains unclear. In this study, we found that ALA significantly promoted sucrose accumulation and SPS (sucrose phosphate synthase) activity in peach fruit. At 14, 28, 42, 50 and 60 days after ALA treatment, sucrose content of fruit was increased by 23%, 43%, 37%, 40% and 16%, respectively, compared with control treatment, and SPS enzyme activity was increased by 21%, 28%, 47%, 37% and 29%, respectively. Correlation analysis showed that the sucrose content of peach fruit under ALA treatment was significantly positively correlated with SPS activity. Subsequently, bioinformatics was used to identify SPS gene family members in peach fruit, and it was found that there were four members of the PpSPS gene family, distributed on chromosomes 1, 7 and 8, named PpSPS1, PpSPS2, PpSPS3 and PpSPS4, respectively. The results of qRT-PCR showed that PpSPS2 and PpSPS3 were highly expressed in response to ALA during fruit development, and the expression of PpSPS2 was positively correlated with SPS activity and sucrose accumulation in peach fruit. The results of tobacco subcellular localization showed that PpSPS2 was mainly distributed in the cytoplasm and nucleus, while PpSPS3 was mainly distributed in the nucleus. The results of this study will lay the foundation for further study on the functions of PpSPS and the regulation of sugar metabolism during the development and ripening of peach fruit by ALA.

Assessment of apple soluble sugar content by hyperspectral vegetation indexes

Soluble sugar content in apple fruit is a crucial internal quality factor. Rapid and accurate estimation of soluble sugar content in apple fruit is helpful to predicting maturity. However, few studies have used an approach of hyperspectral vegetation indexes for monitoring apple soluble sugar content from ground-based handheld hyperspectral data. This study systematically analyzed the performance of hyperspectral vegetation indexes of apple in estimating the soluble sugar content. The visible and near infrared spectral of apple samples were measured by using a hyperspectral equipment, with the wavelength of 350–2500 nm. Eleven early-ripening apple varieties were collected from orchards located in five provinces of China, and the sugar content of each apple fruit sample was measured using laboratory methods. Two-band combination method was used to identify the effective spectral index. Results showed that the newly developed two-band ratio spectral index R833/R834 (Rλ is the reflectance value at wavelength λ) was recommended as the optimal index for monitoring early-ripening apple soluble sugar content, generated the coefficients of determination, root mean square error (RMSE) and residual prediction deviation (RPD) values between the measured and predicted values of 0.73%, 0.72%, and 1.38%, respectively. Result indicated that the newly developed two-band index R833/R834 can be used to nondestructively determine apple fruit soluble sugar content. The findings can provide insight into the relationship between the optical properties of apple fruit at different wavelengths and soluble sugar content.

The Effect Plant Media Composition on the Growth and Production Some Varieties Strawberry

This research was conducted in Barus Jahe Village, Barus Jahe District, Karo Regency, North Sumatra, with an altitude of 1,367 meters above sea level. This study aims to determine the effect of the composition of the planting medium and several varieties on the production and growth of strawberry plants. The design used in this research is a Factorial Split Plot Design (RPT), which consists of two factors. The first factor is the main plot, Variety (V), with 3 levels: V1 = California, V2 = Local Bandung, and V3 = Local Brastagi. The second factor is the subplot, namely Planting Media Composition (M), which consists of Soil: Charcoal Husk: Chicken Manure, namely: M1= 1:1:1, M2= 2:1:1, M3= 1:2:1, and M4= 2:1:2.The variables observed were plant height, plant wet weight and plant dry weight, fruit weight and sugar content (Brix). Analysis of variance was used to test the effect of treatment and mean differences between treatments; honest significant difference tests, regression, and correlation were carried out at a test level of 5%. The research results showed that the composition of the planting media had no significant effect on the observed parameters. However, in some varieties, V1 (California) has a substantial impact on fruit weight and sugar content (Brix), and V2 (Local Bandung) has a significant effect on plant height, wet weight, and dry weight.

Effect of Organic Manure and Biofertilizers on the Growth, Yield and Productivity of Guava under Meadow Orcharding

The study was conducted to investigate the effect of organic manure and biofertilizers on the growth, yield and productivity of guava under meadow orcharding at Horticulture Research Farm, Department of Agronomy, Naini Agricultural Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj. In this experiment, various organic sources (Farm yard manure (30 kg/plant/y), vermicompost (30 kg/plant/y) and neem cake (3 kg/plant/y) along with various biofertilizer combinations (Azotobacter, Azospirillum, PSB) were tested on five-year-old guava cultivar 'Allahabad Safeda' to study its effect on growth, fruiting and yield. The results obtained showed higher fruit weight (218.00 g), fruit length (7.60 cm), fruit diameter (7.42 cm), maximum number of fruits produced per plant (26.64 fruits/plant), maximum yield per plant of 5.81 kg/plant and maximum yield per ha (23.12 t/ha) with the application of T9. The highest total soluble solids (12°Brix) and total sugar content of fruit (8.26%) with the application of T9. Application of nutrients through organic along with biofertilizers improve soil health in terms of mean microbial population in the rhizosphere of root zone soil as compared to control. From the present study, the application of biofertilizers with the combination of organic manures has a beneficial effect on the availability of major nutrients in the soil to help in maintaining of the soil fertility and plant growth of the fruits at a desired level. It can be concluded that, for better growth, yield and quality guava fruits, the guava trees should be fertilized with (FYM (30kg/plant) +Azospirillum @250g/tree +PSB @250g/tree +Neem cake @3kg/tree) per year with treatment T9.

Hydrogen sulfide alleviates the chilling-induced lignification in loquat fruit by regulating shikimate, phenylpropanoid and cell wall metabolisms

Lignification is a typical symptom of chilling injury (CI) in loquat fruit under chilling stress. This study investigated the impact of hydrogen sulfide (H2S) on endogenous H2S, shikimate, phenylpropanoid, and cell wall metabolisms of loquat fruit during refrigeration. The findings suggested that H2S treatment hindered the rise in the CI index and firmness while delaying the reduction in extractable juice, soluble sugar, and titratable acid content of loquat fruit. Besides, H2S treatment promoted the endogenous H2S content, which was correlated with the increase in activities of related H2S synthase enzymes. Furthermore, H2S suppressed enzymes involved in shikimate and phenylpropanoid metabolisms, thereby decreasing phenylalanine and lignin accumulation. Simultaneously, H2S treatment retarded the increases in insoluble pectin, hemicellulose, and cellulose by orchestrating cell wall-modifying enzymes. This work indicates that H2S treatment may alleviate chilling-induced lignification by inhibiting lignin accumulation and enhancing the depolymerization and solubilization of cell wall polysaccharides.