Oat β-glucan reshapes gut microbiota to enhance glucose homeostasis via coordinated modulation of bile acid conjugation and succinate-dependent intestinal gluconeogenesis.

In situ Ca²⁺‑cross‑linking effects on pectin-tragacanth films for fresh raspberry preservation.

Effects of population density stress on fecal microbiota and metabolites of Qinghai-Tibet Plateau root voles (Microtus oeconomus)—A field experiment

Functional Lactiplantibacillus plantarum MT213 screened from traditional sourdough and its promising in-situ preservation in steamed bread.

Dietary Aspergillus niger filtrate: A natural supplement to boost quail breeder productivity and health.

Synergistic phosphorus-carboxyl co-modification of dolomite for enhanced immobilization and long-term stabilization of Pb in acidic contaminated soils.

In-situ selective leaching and closed-loop recovery from waste LiFePO4 batteries by reusable succinic acid

Differential metabolic responses to hydrogen peroxide-induced oxidative stress in parotid and submandibular gland acinar cell lines.

Although lychee peel extract (LPE) is rich in bioactive compounds, its potential for postharvest fruit preservation remains unexplored. We hypothesised that LPE would act synergistically with chitosan (CH) to delay mango ripening by simultaneously modulating cell wall integrity, pigment metabolism, and hormone signaling pathways. Here, we demonstrate that chitosan combined with lychee peel extract (CHL) delays mango ripening through a multi-targeted mechanism. Specifically, CHL outperformed chitosan alone by significantly suppressing peel yellowing, maintaining fruit firmness, and reducing decay over 12days of storage. Integrated transcriptomic and metabolomic analyses revealed that LPE reprogrammed ripening-associated pathways by (1) upregulating cell wall remodeling genes (CSLE1, XTH23) to stabilize pectin architecture, (2) retaining chlorophyll via suppressed CRTISO and PSY (carotenoid synthesis) and enhanced CHLP (chlorophyll biosynthesis), and (3) decoupling sugar-acid dynamics through γ-aminobutyric acid (GABA) and succinic acid accumulation. Notably, LPE attenuated ethylene-auxin- abscisic acid (ABA) crosstalk by downregulating ripening-specific transcription factors (ERF003, bZIPs) while activating stress-responsive WRKYs. These findings establish LPE as a sustainable alternative to synthetic preservatives, leveraging agricultural byproducts for eco-friendly fruit preservation.

Bacillus subtilis is a spore-forming bacterium commonly implicated in food spoilage and foodborne illnesses due to its resistance to harsh conditions. Upon exposure to favorable environments, the spores germinate and resume metabolic activity and thus pose a risk to food safety. Detecting early germination stages is thus crucial for preventing contamination and subsequent illness outbreaks. This study evaluated the potential of nuclear magnetic resonance (NMR)-based metabolomics to identify significant metabolites released during Bacillus subtilis OSU 494 spore germination using two nutrient germinants. The goal was to uncover biomarkers that could support improved detection strategies in food safety applications. The spores were induced to germinate using either tryptic soy broth (TSB) or L-asparagine, D-glucose, D-fructose, and potassium chloride (AGFK). The samples were collected hourly over 4h and analyzed using an 850MHz NMR spectrometer with a triple-resonance cryoprobe. 1D-¹H NOESY and 2D ¹H-¹³C HSQC spectra were obtained. Spectral binning and linear modeling were then applied to identify significant metabolic features. The AGFK-induced germination yielded dipicolinic acid (DPA), L-alanine, acetic acid, L-phenylalanine, and formic, succinic, and fumaric acids. The TSB-induced germination produced DPA, L-alanine, L-phenylalanine, acetic and fumaric acids. Several metabolites were consistently released during germination in both nutrient conditions. These metabolites, particularly DPA and L-alanine, served as reliable biomarkers for the Bacillus subtilis spore germination. They then provided valuable insights for developing rapid detection tools to enhance food safety monitoring and contamination control.

This study presents a modified and integrated process for succinic acid production from lignocellulosic biomass wastes, incorporating pretreatment, enzymatic hydrolysis, and fermentation. Oil palm empty fruit bunches (OPEFB) and sugarcane bagasse (SB) were employed as representative biomass feedstocks. Comprehensive kinetic analysis was performed to evaluate microbial growth, product formation, and substrate consumption behaviors during fermentation. Pretreatment was conducted using a sequential combination of peracetic acid and alkaline peroxide solutions, assisted by ultrasonication, to enhance cellulose accessibility and reduce lignin content. The pretreated biomass was subsequently subjected to fermentation using two configurations: simultaneous saccharification and fermentation (SSF) for 48h at 37°C, and semi-simultaneous saccharification and fermentation (SSSF), consisting of a 6h pre-hydrolysis step followed by 48h of SSF. Kinetic analysis showed that microbial growth followed logistic behavior, succinic acid formation was well described by the modified logistic model, and substrate utilization was accurately captured by the modified Gompertz model. Variations in kinetic parameters among the biomass feedstocks highlight the influence of lignin removal efficiency and cellulose accessibility on fermentation performance.

Enhancing the production of high-value metabolites in microalgae is essential for sustainable bioproducts and biofuels. In this study, Porphyridium purpureum cultures were supplemented with β-cyclodextrin (β-CD) and succinic acid (SA) to evaluate their effects on biomass, lipid accumulation, fatty acid profiles, phycobiliproteins, and exopolysaccharides. Moderate β-CD concentrations (0.25-0.50g L- 1) significantly increased biomass (2.98 ± 0.12g L- 1), lipid content (277.00 ± 5.56 mg g- 1), total fatty acids, and arachidonic acid yield (70.60 ± 5.70 mg L- 1) compared to the control. SA supplementation (0.30-0.60g L- 1) selectively enhanced long-chain polyunsaturated fatty acids accumulation compared with the control treatment, with arachidonic acid reaching 45.89 ± 5.70 mg L- 1. Both supplements also elevated phycobiliproteins and exopolysaccharides, with phycoerythrin being the dominant phycobiliprotein, reaching 146.88 ± 12.40 mg L- 1 under 0.50g L- 1 β-CD and 147.00 ± 7.50 mg L- 1 under 0.60g L- 1 SA. Excessive levels of either supplement, however, negatively affected growth and metabolite productivity. These findings demonstrate that controlled β-CD and SA supplementation can efficiently improve biomass and the accumulation of lipids, long-chain PUFAs, PBPs, and EPS in P. purpureum, offering a practical strategy for industrial-scale production of high-value algal compounds.

Semi-organic amino acid-based crystals have great potential to be used as optical materials because configurationally tunable hydrogen-bond networks and natural molecular polarity can be utilised. Nevertheless, a mechanistic understanding of the hydrogen bond interaction with the band gap is still scarce in detail. In this work, we discuss L-glycinesuccinate dihydrate (LGSD) as a model hydrogen-bond regulated optical system and intend to discuss how full proton transfer is responsible for electronics decoupling and transparency enhancement. Amino salt structure and properties. Spectroscopic and structural studies confirm the formation of a stabilised amino-salt that contains NH3+ and COO- moieties that give a sharp optical cutoff at ~ 200nm and a wide-transfer transparency interval in the visible range. Tauc analysis suggests a direct band gap of 6.42eV, which indicates a wide gap optical insulator with no absorption in the mid gap. Thermogravimetric, differential thermal analysis and diffraction measurements were performed to ascertain solvent-free lattice stabilisation and superior crystallinity of the material to validate the optical reliability. LGSD crystals were obtained by slow evaporation of a stoichiometric aqueous solution of L-glycine and succinic acid. The structural, optical and thermal properties of the processed nanomaterials were characterised using Fourier-transform infrared spectroscopy, ultraviolet-visible-near-infrared spectroscopy (Tauc analysis), fluorescence spectroscopy, thermogravimetric analysis/differential thermal analysis/powder X-ray diffraction and scanning electron microscopy.

Cocrystal mitigates the effects of elevated gastric pH on oral absorption of weakly basic drugs: a case study with ketoconazole-succinic acid cocrystal on beagle dogs.

Construction of a rapid succinate concentration detection system based on the specific catalysis of succinate dehydrogenase.

Comprehensive evaluation of lactic acid bacteria and yeast fermentation on the physical, nutritional and flavor quality of vacuum-freezing-hot-air-dried sweet potato powders.

Biological CO2-capture technologies, such as biogas upgrading, constitute essential tools toward decarbonization of the chemical and energy industries. Biological succinic acid production is one such process that naturally fixes CO2 while producing an important platform chemical for the chemical and food industries. However, the high costs associated with biosuccinic acid production render it economically uncompetitive compared to petrochemically produced succinic acid. Here, we propose an integrated platform combining fermentation for succinic acid production from industrial waste streams with the successful upgrade of raw biogas from anaerobic digestion, which repurposes the process into a multi-product platform for increasing its economic viability. To reduce downstream separation costs, the fermentation process is also coupled with an in situ separation module that increases the performance of both the succinic acid fermentation and the biogas upgrade, reaching a CH4 percentage of 93% in the final biogas and triggering a 32.5% increase in succinic acid production. Moreover, the use of the electrochemical module aids in the separation of succinic acid from the fermentation broth, resulting in 94.80% recovery of the succinic acid produced. All experiments were performed at semi-pilot scale.

Succinic acid has been considered an important molecule in the transition of chemical manufacturing from fossil-based to sustainable and future-proof processes. While there has been extensive research on biotechnological succinic acid production from biomass, attempts to roll out bio-succinic acid are impeded by its high price and remaining sustainability issues. Both drawbacks are interconnected and can be traced back to the used feedstocks and a wasteful expenditure of acid and base, among others. In this opinion, we discuss biochemical principles and metabolic pathways of next-generation carbon assimilation and low-pH fermentations to address these drawbacks. For this reason, we chart the potential for producing succinic acid from sustainable next-generation feedstocks based on electron, carbon and ATP balances as well as relevant thermodynamic considerations. Furthermore, we summarize key advances in low-pH succinic acid synthesis using acid-tolerant yeasts and assess the suitability of selected acid tolerance mechanisms for industrial application. Eventually, we aim to inspire researchers to synthesize innovative approaches to realize competitive and sustainable biotechnological succinic acid production.

Anthropogenic nitrogen (N) deposition is reshaping terrestrial phosphorus (P) cycling. This effect is pronounced in the rhizosphere, where plants respond to P status by releasing root exudates. Yet it remains unclear whether these interactions drive P mobilization or immobilization. To capture both the chronic effects of N deposition and the transient effects of root exudates, we integrated a decade-long N addition experiment (a single annual application of (NH4)2SO4 at 0, 2, and 5 g N m-2 year-1) with a 14-day laboratory incubation. Specifically, rhizosphere and bulk soils from a Eurasian meadow steppe were amended with 13C-labeled low-molecular-weight organic acids (LMWOA; a single addition of acetic and succinic acids). Phosphorus transformation was quantified using sequential extraction and 31P NMR, while underlying mechanisms were assessed via priming effects, phosphatase activity, and microbial functional genes. Nitrogen enrichment promoted the dissolution of mineral-bound inorganic P (Pi) through acidification (pH drop > 1 unit), leading to the accumulation of secondary mineral Pi and organic P (Po). This was accompanied by reduced microbial phosphatase activity and lower abundances of the P-cycling genes phoC and phoD. The above effects were stronger in the rhizosphere than in bulk soil. In the rhizosphere, LMWOA enhanced microbial P immobilization and Po accumulation, even as their positive priming effects accelerated soil organic carbon mineralization. These results were partly driven by the recruitment of key P-cycling taxa such as Burkholderia and Rhodoplanes, suggesting a shift in carbon-for-P exchange between plants and microbes under N deposition. Collectively, our findings indicated that while N-induced P solubilization may temporarily alleviate P limitation, synergy with increased LMWOA exudation promotes Po accumulation. Though Po can be labile compared to mineral-bound forms, suppressed phosphatase activity likely constrains its mineralization. This inhibition potentially decelerates P cycling, thereby compromising the medium- to long-term P supply within the Eurasian steppe.

Classification of honey types via flow profile analysis on μPAD combined with physicochemical characterization of honey-chitosan mixtures.