Succinate Medium Research Articles

Anticandidal Activity of a Siderophore from Marine Endophyte Pseudomonas aeruginosa Mgrv7.

An endophytic symbiont P. aeruginosa-producing anticandidal siderophore was recovered from mangrove leaves for the first time. Production was optimal in a succinate medium supplemented with 0.4% citric acid and 15 µM iron at pH 7 and 35 °C after 60 h of fermentation. UV spectra of the acidic preparation after purification with Amberlite XAD-4 resin gave a peak at 400 nm, while the neutralized form gave a peak at 360 nm. A prominent peak with RP-HPLC was obtained at RT 18.95 min, confirming its homogeneity. It was pH stable at 5.0-9.5 and thermally stable at elevated temperatures, which encourages the possibility of its application in extreme environments. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) against Candida spp. Were in the range of 128 µg/mL and lower. It enhanced the intracellular iron accumulation with 3.2-4.2-fold (as judged by atomic absorption spectrometry) with a subsequent increase in the intracellular antioxidative enzymes SOD and CAT. Furthermore, the malondialdehyde (MDA) concentration due to cellular lipid peroxidation increased to 3.8-fold and 7.3-fold in C. albicans and C. tropicalis, respectively. The scanning electron microscope (SEM) confirmed cellular damage in the form of roughness, malformation, and production of defensive exopolysaccharides and/or proteins after exposure to siderophore. In conclusion, this anticandidal siderophore may be a promising biocontrol, nonpolluting agent against waterborne pathogens and pathogens of the skin. It indirectly kills Candida spp. by ferroptosis and mediation of hyperaccumulation of iron rather than directly attacking the cell targets, which triggers the activation of antioxidative enzymes.

EFFECT OF VARIOUS PHYSICOCHEMICAL PARAMETERS ON SIDEROPHORE PRODUCTION BY MARINE Pseudomonas aeruginosa MGPB31

Siderophores are low molecular weight compounds secreted by bacteria that 
 bind ferric iron with extremely high affinity. The siderophores help bacteria to 
 meet their iron needs under stress conditions as these compounds help in the 
 transport and storage of soluble form of iron in cells. In current study, we report 
 the effect of various physicochemical parameters that influence siderophore 
 production in marine Pseudomonas aeruginosa MGPB31. Siderophore 
 production was assayed using Chrome Azurol S (CAS) shuttle assay. The 
 maximum siderophore production (56% SU) was obtained in minimal M9 
 medium after 72 h incubation, followed by succinate medium (52% SU) and 
 King’s medium (51% SU). The M9 medium was then modified by replacing its 
 carbon, nitrogen and phosphate source. Enhanced siderophore production was 
 observed in modified medium comprising of glycerol (70% SU), tri ammonium 
 citrate (67% SU) and KH2PO4 (64% SU). The medium supplemented with iron 
 exhibited a significant reduction in siderophore production. The effect of 
 physical parameters like temperature, pH and agitation on siderophore 
 production revealed that the isolate exhibited maximum siderophore production 
 under static conditions in modified M9 medium devoid of iron at 25 ± 2°C and 
 neutral pH. The enhanced siderophore production obtained by marine P. 
 aeruginosa MGPB31 indicates its further applicability in fields of industry 
 agriculture and environment.

Bioenergetics of the polyphosphates accumulation in Pseudomonas aeruginosa via polyphosphate kinase activation by choline in a lung colonization model

Pseudomonas aeruginosa is an ubiquitous and opportunistic bacteria found in water, soil, plants, and immunocompromised humans. Cystic fibrosis (CF) patients are the most vulnerable population to lung colonization by these bacteria. Upon infection, choline and succinate are released from the CF lungs and are catabolized by P. aeruginosa. The bacteria accumulate inorganic polyphosphates, rather than succinate, when choline is catabolized, producing physiological and morphological changes leading to ineradicable infection. Thus, we sought to quantify the enzymes responsible for polyphosphate accumulation and to determine how choline catabolism affects energy flow and storage. Subcellular fractions showed that exo-polyphosphate phosphatase (PPX) activity resides mainly in the periplasm, and three isoenzymes of 24, 70, and 200 KD were found. The PPX activity in the periplasm of bacteria grown with choline was inhibited in an anti-competitive manner from Km 0.5 to 1 μM, and their Vmax increased from 50 to 100 nmol PO4≡/min/g of protein in succinate medium. Since PPX inhibition by choline did not explain the 3.8-fold increase in polyphosphates, we quantified the polyphosphate kinase activity, and its significant 2.4-fold increase was consistent with the accumulation. Furthermore, intracellular ATP concentration directly correlated with the energetic yield of the carbon source and was significantly higher for succinate, suggesting that the restriction of energy caused by choline catabolism may induce morphological and physiological changes to the swarm form thus facilitating their migration and tissue colonization

Isolation and Characterization of Siderophores Producing Chemolithotrophic Bacteria from the Coal Samples of the Aluminum Industry

Siderophores are iron grabbers and are classified according to the ligands used to chelate the ferric iron. The role of siderophores is to scavenge iron and make it accessible to the cell. It has crucial applications in industries, medical, research, and environmental clean-up. In the current research work, the authors attempted to isolate and characterize a few siderophores producing bacteria from the coal samples, collected from the Bharat Aluminum Company Ltd. Chhattisgarh. The quantitative and qualitative assay for siderophores producing bacteria (SPB) was carried by Chrome Azurol Sulfonate agar test for all the ten samples and positive results were observed only in two strains, i.e., B6 and B7 when grown in iron-deficient succinate medium in an incubator shaker for 48 h at 120 rpm. The biochemical tests revealed that the B6 and B7 strains have similarities to Bacillus sp. and Vibrio sp., respectively. The study reflects the potential of these strains as effective siderophores producers and holds a noteworthy perspective in further industrial and research applications.

REMOVAL OF LEAD (II) WITH POLY 3-(AZA-18-CROWN-6) SILICA BONDED PHASE FROM SUCCINATE MEDIUM AND SAMPLES ANALYSIS

A method was described for the determination of micro amount of lead (II) with Poly 3-(Aza-18-crown-6) Silica bonded phase employed as an ion-pair forming a neutral complex from Sodium succinate medium (0.01 to 3.0 M). The adsorbed ion-pair complex was back eluted with 0.5 M hydrochloric acid and determined spectrophotometrically with PAR. The various parameters like concentration of acid, equilibrium time, back eluting agents, loading capacity were optimized for quantitative adsorption of lead (II). The applicability of the proposed method was successfully applied to the analysis of diverse ions, binary mixture of associated metal ions, ternary mixtures, alloys, ayurvedic samples and water samples and lead (II) was determined with the PAR and results of analysis were confirmed by AAS.

Inhibition of Escherichia coli ATP synthase by dietary ginger phenolics

For centuries, dietary ginger has been known for its antioxidant, anticancer, and antibacterial properties. In the current study, we examined the link between antibacterial properties of 7 dietary ginger phenolics (DGPs)—gingerenone A, 6-gingerol, 8-gingerol, 10-gingerol, paradol, 6-shogaol, and zingerone—and inhibition of bacterial ATP synthase. DGPs caused complete (100%) inhibition of wild-type Escherichia coli membrane-bound F1Fo ATP synthase, but partial and variable (0%–87%) inhibition of phytochemical binding site mutant enzymes αR283D, αE284R, βV265Q, and γT273A. The mutant enzyme ATPase activity was 16-fold to 100-fold lower than that of the wild-type enzyme. The growth of wild-type, null, and mutant strains in the presence of the 7 DGPs were abrogated to variable degrees on limiting glucose and succinate media. DGPs-caused variable inhibitory profiles of wild-type and mutant ATP synthase confirm that residues of α-, β-, and γ-subunits are involved in the formation of phytochemical binding site. The variable degree of growth in the presence of DGPs also indicates the possibility of molecular targets other than ATP synthase. Our results establish that antibacterial properties of DGPs can be linked to the binding and inhibition of bacterial ATP synthase. Therefore, bacterial ATP synthase is a valuable molecular target for DGPs.

Application of Response Surface Methodology for Optimization of Siderophore Production

Background: In the present study a statistical model (Response Surface Methodology) was proposed for optimization of siderophore production by using Enterobacter hormaechei.Methods: The rhizospheric soil was used for isolation and isolates were screened for siderophore production by chrome-azurol S (CAS) assay. One potent isolate producing maximum siderophore was selected and characterized by 16S rRNA gene sequencing. The culture conditions were optimized for maximum siderophore production by using Central Composite Design. The response surface curves were used to predict the optimum levels of the factors affecting the yield of siderophore. Result: By using rhizospheric soil,eight isolates were obtained and one potent organism was identified as Enterobacter hormaechei subsp. oharae (Accession No. MT 775835) by BLAST. The maximum siderophore production (98%) was obtained in succinate medium and the optimum values of variables were found as pH 7, time 60 hrs, temp. 28°C and succinic acid conc. 0.40%. RSM was used to analyze the data by developing 3D surface plots and the residuals plots. ANOVA was used to determine regression coefficients.

Antibacterial activity of fosetyl‐Al, ethyl‐phosphite and phosphite against Pseudomonas syringae on plant surfaces and in vitro

Fosetyl‐Al (the aluminium salt of ethyl‐phosphite) is an acid product used to fight oomycete diseases and sometimes used against bacterial diseases, but its antibacterial mode of action is largely unknown. Therefore, the direct effects of fosetyl‐Al and neutralized fosetyl‐Al on the colonization of leaf surfaces by Pseudomonas syringae were compared. Control of colonization was highest and almost complete in acidic conditions (99.7%), showing the importance of acidity for maximal antibacterial efficacy on plant surfaces. However, the high inhibition obtained with neutralized fosetyl‐Al (88.9%) suggested another direct effect. Therefore, it was investigated in vitro whether ethyl‐phosphite has antibacterial activity, possibly related to its phosphite content. Inhibition of P. syringae growth by ethyl‐phosphite was observed and influenced by the carbon source and by phosphate. Growth inhibition reached 83.1% in unshaken conditions in a phosphate‐depleted glucose medium. Phosphite drastically increased the effects and induced up to 99.7% growth inhibition. Mass spectrometric analysis indicated that P. syringae hydrolysed ethyl‐phosphite to phosphite using a cell‐linked phosphatase activity. Specific phosphite toxicity was observed in phosphate‐sufficient succinate media and phosphite‐induced phosphorus starvation was observed in phosphate‐depleted glucose media, indicating that ethyl‐phosphite and phosphite have direct environmental impacts on bacteria. Phosphite toxicity for P. syringae probably occurred in the phyllosphere. The study indicates the potential and limitations of fosetyl‐Al as a direct antibacterial product and helps in understanding the fosetyl‐Al control of flower bud blast in pear. It raises the prospect of using phosphite in plant bacterial disease control.

Characterization and optimization of bacterium isolated from soil samples for the production of siderophores

Siderophores are small molecules that can easily bind to ferric iron. As a chelating agent, they transportiron molecules inside the bacterial cell for various biochemical reactions. Due to its various applicationsin medicinal, industrial and environmental related aspects, this paper deals with characterizationand optimization of few siderophores producing bacteria from the soil samples, collected from Chikkamagalurudistrict, Karnataka. The siderophores production was assayed qualitatively and quantitativelythrough Chrome Azurol S and the results showed positive for the strains VIT VK5 and VIT VK6 that grownin succinate medium. Further characterization and optimization results revealed that both the bacteriumhas the ability to yield siderophores ( ∼60–80% units) in the optimum condition of pH 8, at 37 °C withglucose and sucrose as a carbon source and NaNO3 as a nitrogen source. Thus, the study concludes thatstrains VITVK5 and VITVK6 can be promising candidates for the siderophores production which can playmajor applications in medicinal and industrial aspects.

Diversity of the auxotrophic requirements in natural isolates of Escherichia coli.

Isolates of Escherichia coli, except Shigella, are generally prototrophic; they do not require any growth factors to grow in mineral medium. However, a nicotinic acid requirement is common among B2 phylogroup STc95 O18 E. coli clone strains. Nicotinic acid is a precursor of nicotinamide adenine dinucleotide (NAD), an essential molecule that plays central role in cellular metabolism. The defect in NAD synthesis of these strains is due to alterations in de novo biosynthesis pathway nadB gene. Here, by studying growth on minimal medium with glycolytic (glucose) or gluconeogenic (pyruvate or succinate) substrates as the carbon supply in a large panel of E. coli natural isolates representative of the species diversity, we identify an absolute nicotinic acid requirement in non-STc95 strains due in one case to a nadA inactivation. The growth on glucose medium of some extraintestinal pathogenic E. coli strains belonging to various non-O18 B2 phylogroup STc95 clones is restored either by aspartate or nicotinate, demonstrating that the nicotinic acid requirement can also be due to an intracellular aspartate depletion. The auxotrophic requirements depend on the carbon source available in the environment. Moreover, some strains prototrophic in glucose medium become auxotrophic in succinate medium, and conversely, some strainsauxotrophic in glucose medium become prototrophic in succinate medium. Finally, a partial depletion of intracellular aspartate can be observed in some prototrophic strains belonging to various phylogroups. The observed more or less significant depletion according to isolates may be due to differences in tricarboxylic acid cycle enzyme activities. These metabolic defects could be involved in the adaptation of E. coli to its various niches.

Involvement of lipopeptide antibiotics and chitinase genes and induction of host defense in suppression of Fusarium wilt by endophytic Bacillus spp. in tomato

Five endophytic Bacillus spp. isolated from surface-sterilized stem tissues of wild Solanaceous species, i.e. Datura metel, Solanum nigrum and S. elaeagnifolium, were screened for their in vitro and in vivo antagonistic potential against Fusarium oxysporum f. sp. lycopersici (FOL). Pathogen sporulation was totally suppressed by these five strains and its mycelial growth was significantly decreased using bacterial whole-cell suspensions and cell-free culture filtrates. Extracellular metabolites remained effective after heating at 50–100 °C with a decline in activity with treatments involving temperatures at 100 °C, proteinase K addition, and pH adjustments to 2 or 12. Chitinase and lipopeptide antibiotic genes were detected using PCR amplification. The five strains produced salicylic acid when grown in succinate medium, with the greatest production exhibited by B. subtilis str. SV41. In planta application of Bacillus spp. cell-free culture filtrates and whole-cell suspensions significantly decreased Fusarium wilt severity by 87–100% and enhanced tomato growth by 38–80%, compared to FOL-inoculated and untreated controls. The two Bacillus strains exhibiting the strongest decrease in Fusarium wilt severity (B. subtilis str. SV41 and B. amyloliquefaciens subsp. plantarum str. SV65) were further evaluated for their ability to induce systemic resistance (ISR) in FOL-infected and uninfected tomato plants. Using quantitative RT-PCR, the expression of the acidic PR-1 and PR-3 genes as well as the lipoxygenase (LOXD) gene were significantly induced in plants treated with B. amyloliquefaciens subsp. plantarum str. SV65. This effect was observed in both FOL-inoculated and uninoculated plants.

Bioremediation capability and characterization of bacteria isolated from petroleum contaminated soils in Iran

Abstract This study was carried out to isolate bacteria for bioremediation of petroleum polluted soils. Five samples were used for isolation in this study. They were four soil samples in addition to one kerosene sample. The soil samples including soils contaminated by crude oil and gas oil and two soil samples with no outward contamination which were collected from Shiraz Oil Refinery sites. Seven strains were selected among the isolated colonies for further experiments. The selected isolates were cultured in standard succinate medium (SSM) minimal medium in which 2.5% v/v kerosene was used as carbon source. In another bacterial SSM culture, carbon, sulfur or nitrogen source was removed and 20% v/v kerosene added to check the ability of isolates to utilizekerosene as sole source for C, N and S. Finally, cultures of four strains with higher growth in modified SSM cultures were selected for GC analysis. In this study they were named C2 and C4 which were isolated from crude oil contaminated soil and SI1 and SI2 isolated from soils with no outward contamination. GC analysis showed that C2 could degrade 69% of 5% v/v kerosene in 7 d, while C4 and SI1 degraded 48% and 42% of 5% v/v kerosene during this 7-d period respectively, and the degradation ability of SI2 was 38% after 7 d. Analysis of 16S rRNA gene showed that C2 was close to Citrobacter sedlakii, C4 and SI1 were related to Entrobacter hormeachei and SI2 was close to Entrobacter cloacae, respectively.

Characterization and optimization of bacterium isolated from soil samples for the production of siderophores

Abstract Siderophores are small molecules that can easily bind to ferric iron. As a chelating agent, they transport iron molecules inside the bacterial cell for various biochemical reactions. Due to its various applications in medicinal, industrial and environmental related aspects, this paper deals with characterization and optimization of few siderophores producing bacteria from the soil samples, collected from Chikkamagaluru district, Karnataka. The siderophores production was assayed qualitatively and quantitatively through Chrome Azurol S and the results showed positive for the strains VITVK5 and VITVK6 that grown in succinate medium. Further characterization and optimization results revealed that both the bacterium has the ability to yield siderophores (∼60–80% units) in the optimum condition of pH 8, at 37 °C with glucose and sucrose as a carbon source and NaNO3 as a nitrogen source. Thus, the study concludes that strains VITVK5 and VITVK6 can be promising candidates for the siderophores production which can play major applications in medicinal and industrial aspects.

Development of liquid-liquid extraction and separation method for ruthenium(III) with 2-octylaminopyridine from succinate media: Analysis of catalysts

Ruthenium(III) has been efficiently extracted from 0.05 M sodium succinate at pH 9.5 by 2-octylaminopyridine in xylene and stripped with aqueous 10% (w/v) thiourea solution and determined spectrophotometrically. Various parameters viz., pH, weak acid concentration, reagent concentration, stripping agents, contact time, loading capacity, aq.: org. volume ratio, solvent has been thoroughly investigated for quantitative extraction of ruthenium(III). The utility of method was analyzed by separating the ruthenium(III) from binary mixture along with the base metals like Cu(II), Ag(I), Fe(II), Co(II), Bi(III), Zn(II), Ni(II), Se(IV), Te(IV), Al(III) and Hg(II) as well as platinum group metals (PGMs). Ruthenium(III) was also separated from ternary mixtures like Os(VIII), Pd(II); Pd(II), Pt(IV); Pd(II), Au(III); Pd(II), Cu(II); Fe(II), Cu(II); Ni(II), Cu(II); Co(II), Ni(II); Se(IV), Te(IV); Rh(III), Pd(II); Fe(III), Os(VIII). The stoichiometry 1: 2: 1 (metal: succinate: extractant) of the proposed complex was determined by slope analysis method by plotting graph of logD[Ru(III)] versus logC[2-OAP] and logD[Ru(III)] versus logC[succinate]. The interference of various cations and anions has been studied in detail and the statistical evaluations of the experimental results are reported. The method was successfully applied for the analysis of ruthenium in various catalysts, synthetic mixtures corresponding to the composition of alloys and minerals.

Kerosene biodegradation ability and characterization of bacteria isolated from oil-polluted soil and water

With increasing production and consumption of oil, the inevitable spillage of oil presents a significant challenge to protecting the environment and human health. Bioremediation is an effective approach to address this challenge. In this research, three soil and two wastewater samples contaminated by petroleum and a sample of crude oil were collected from Bandar Abbas Refinery, Iran. These samples were used for isolation and identification of bacteria, which can be used for cleaning polluted lands. Twelve strains were isolated and cultured at 28°C standard succinate medium from which carbon or sulfur resources were eliminated and kerosene was added. Three isolates were selected for identification because of their high growth rate in kerosene tests. The biodegradation activity of these bacteria was analyzed by gas chromatography. Using biochemical tests, 16S rDNA sequence, and API 20 E kit, it was revealed that these bacteria belong to Enterobacter cloacae, Enterobacter hormaechei, and Pseudomonas stutzeri. They were able to degrade 67.43%, 48.48%, and 65.48% of 5% kerosene as carbon source in seven days, respectively. Pseudomonas stutzeri and Enterobacter hormaechei could respectively degrade 54.14% and 12.98% of 10% kerosene as sulfur source in seven days. Hence, these bacteria can be considered as excellent candidates for petroleum biodegradation.

Siderophore production by Pseudomonas aeruginosa FP6, a biocontrol strain for Rhizoctonia solani and Colletotrichum gloeosporioides causing diseases in chilli

Siderophores are compounds secreted under low iron stress, which act as specific ferric iron chelating agents. Owing to their potential in the biological control of fungal phytopathogens, they may be used as an alternative strategy to chemical control. Pseudomonas aeruginosa FP6, previously isolated from rhizospheric soil samples was screened for its siderophore production on a chrome-azurol S agar plate. Change in the colour of the chrome-azurol S agar from blue to orange red confirmed the siderophore producing ability of P. aeruginosa FP6. The effects of various physicochemical parameters on siderophore production were studied. The maximum siderophore production was obtained in succinate medium (125 μM) followed by King's B medium (105 μM). The presence of sucrose and mannitol increased the siderophore production. Yeast extract proved to be the most suitable nitrogen source. Media supplemented with Pb2+, Mn2+ and Mg2+ showed appreciable siderophore production as well as growth of cultures. An increase in the iron concentration favoured growth but substantially reduced siderophore production. The strain when tested for its in-vitro antagonistic activity against Rhizoctonia solani and Colletotrichum gloeosporioides on King's B media, with and without FeCl3, showed a significant reduction in R. solani growth with FeCl3 supplementation compared to the control (without FeCl3), suggesting the role of siderophore mediated antagonism of R. solani. Antifungal activity was not influenced by FeCl3 in the case of C. gloeosporioides, suggesting the presence of other antagonistic mechanisms.

Statistical-based optimization and scale-up of siderophore production process on laboratory bioreactor.

We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett–Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid, pH and temperature were found as significant components that influenced the siderophore production in Pseudomonas aeruginosa RZS9. The second stage of RSM using CCD consisted of optimizing the concentrations of the variables. Here, 0.49 g/100 ml concentration of succinic acid, pH 7.08 and temperature of 27.80 °C yielded the maximum (68.41 %) siderophore units. All the significant components exhibited quadratic effect on siderophore production. The F value of 28.63, multiple correlation coefficient (R 2) of 0.9626, percent coefficient of variation of 8.81 values indicated that the model was significant and that the experimental data was satisfactorily adjusted to the quadratic model. During validation of these experiments, 6.10 % increase in siderophore yield was obtained. Scale-up of this protocol optimized at shake flask level up to 5 L-capacity reactor further enhanced the siderophore yield. We claim it to be the first report on statistical optimization of siderophore production by P. aeruginosa RZS9.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0365-2) contains supplementary material, which is available to authorized users.

Bioprospecting of pseudomonas aeruginosa for their potential to produce siderophore, process optimization and evaluation of its bioactivity.

Siderophores produced by Pseudomonas spp . play a vital role in controlling several plant diseases. In this study, two siderophore producing bacterial strains were isolated from waste water sample and labeled them as RM1 and RM2 . Among these two isolates, RM1 strain was found to produce more siderophore and therefore selected for further study. 16s rRNA analysis and biochemical characterization as described in the Bergey’s manual of determinative bacteriology, identified the RM1 strain as Pseudomonas aeruginosa 6A (bc4). Siderophore production was determined by more sensitive and reliable Chrome azurol- S (CAS) method. Chemical and spectrophotometric assays showed that P. aeruginosa 6A strain produced 99% siderophore units. Two siderophores, hydroxamate (Pyoverdine) and phenol catecholate (pyochelin) were mainly found to be produced by this strain. Different parameters such as temperature, pH, carbon and nitrogen sources were optimized for maximum production of siderophores. Maximum production (95.65%) was observed on succinate medium, pH 7.0 and at 40⁰c temperature. Several amino acids were used as carbon and nitrogen sources. Among them serine resulted in a maximum production of siderophore (99.80% unit). The Pseudomonas spp . were also tested as seed inoculants and found to be very effective in seed germination and plant growth promotion of Glycine max JS-335 plant under pot culture conditions. It also exhibits antifungal activity against Aspergilus spp . In summary, the present study has identified a novel siderophore producing bacterial strain, which can also be used as an antifungal agent.

Spectrophotometric determination of ruthenium(III) with N-decylpyridine-4- amine from succinate media

Spectrophotometric determination of ruthenium(III) with N-decylpyridine-4- amine from succinate media

Biocontrol activity of siderophore producing Bacillus subtilis CTS-G24 against wilt and dry root rot causing fungi in chickpea

Siderophore producing bacteria as biocontrol agent may play a pivotal role in controlling several fungal pathogens which causes plant diseases. Fusarium oxysporium f. sp. ciceri (FOC) and Macrophomina phaseolina are major phytopathogens amongst the several constraints affecting the productivity of chickpea in India and other countries in Asia. In the present study, indigenous Bacillus subtilis CTS-G24, (Partial 16 S rRNA sequence of Bacillus subtilis is deposited in GenBank with accession number KF322037) was detected positive for siderophore production. Antiphytopathogenic activity of strain CTS-G24 against wilt and dry root rot causing FOC and M. phaseolina respectively was checked. It was revealed that Hydroxamate type of siderophore was produced by CTS-G24 and 64 % of siderophore units was produced in Succinate medium by Bacillus subtilis CTS-G24. Plant Growth Promoting Rhizobacteria (PGPR) are known to influence plant growth by suppressing plant disease and might be applied in agriculture as an alternative to chemical pesticides for improving the quality and yield of crop. Siderophores produced by Bacillus subtilis has got the potentials to