Subsequent Replantation Research Articles

Therapeutic approach in periimplantitis

Peri–implantitis is a frequent a polyetiological disease. Currently its treatment is a challenge, the treatment algorithms still being unclear. Purpose of the study: Evaluation of etiological factors of complications in implant– prosthetic rehabilitation and comparative analysis of available management methods. Materials and methods: 47 patients and 108 implants were included in the study. The parameters of interest were: gender, age of the patients, number of affected implants, spreading of peri–implantitis according to the teeth position and distribution between the jaws, possible etiological factors, distribution of defects according to Schwarz classification, level of bone resorption, applied treatment. Results: Among 34 treated implants, an improvement was achieved in 6 cases, among 72 extracted implants favourable conditions were obtained in all cases. Priority is given to maximum bone preservation and the possibility of subsequent replantation. Radical surgical treatment in extensive defects shows greater predictability than conservative treatment.

24-hour Perfusion of Porcine Myocutaneous Flaps Mitigates Reperfusion Injury: A 7-day Follow-up Study.

Background:Static cold storage is the gold standard of preservation in vascularized composite allotransplantation and allows a preservation time of 4–6 hours. Machine preservation is a promising technique for prolonged preservation; however, studies on extended preservation that compare different preservatives are scarce. This study aims to assess the feasibility of 24-hour acellular perfusion and compares different preservation solutions in a porcine myocutaneous flap replantation model.Methods:Six harvested bilateral myocutaneous flaps of three Dutch Landrace pigs were perfused hypothermically for 24 hours with University of Wisconsin machine perfusion solution (UW-MPS; n = 2) or histidine-tryptophan-ketoglutarate solution (HTK; n = 2) or preserved on ice for 4 hours (n = 2) before orthotopic replantation. Animals were observed for 7 days after replantation. Skeletal muscle injury was assessed by biochemical markers during perfusion, and muscle biopsies were analyzed for ischemia reperfusion injury directly after preservation and at 1, 3, and 7 days after replantation.Results:Markers of muscle damage varied during perfusion, but decreased overall in both perfusion groups. Flap weight increased 60% and 97% in the HTK-perfused flaps, compared with -6% and -7% in the UW-MPS-perfused flaps after 24 hours. Histopathologic evaluation demonstrated decreased muscle damage in flaps perfused with HTK compared with the UW-MPS-perfused flaps at 1 week after replantation.Conclusions:Machine perfusion of myocutaneous flaps for 24 hours with subsequent replantation is feasible, but warrants further research. Perfusion with HTK solution seemed to result in better histological outcomes 7 days after reperfusion compared with UW-MPS.

Clinical procedures and outcome of surgical extrusion, intentional replantation and tooth autotransplantation - a narrative review.

Surgical extrusion is defined as the procedure in which the remaining tooth structure is repositioned at a more coronal/supragingival position in the same socket in which the tooth was located originally. Intentional replantation is defined as the deliberate extraction of a tooth and after evaluation of root surfaces, endodontic manipulation and repair, placement of the tooth back into its original position. Tooth autotransplantation is defined as the transplantation of an unerupted or erupted tooth in the same individual, from one site to another extraction site or a new surgically prepared socket. The advent of titanium implant rehabilitation has reduced the use of these treatments in day-by-day clinical practice; however, the re-emerging trend to conserve and preserve natural sound tissues has led to a rediscovery of these treatments. All three distinct surgical methods are closely related, as they act to treat teeth that cannot be predictably treated using other more conventional procedures in endodontics, periodontics and restorative dentistry. Furthermore, these procedures share the same treatment approach and include the atraumatic extraction of a tooth, visual inspection of the tooth/root and its subsequent replantation. The clinical procedures for surgical extrusion, intentional replantation and tooth autotransplantation treatment have undergone several changes in recent years, and currently, there are no clear clinical treatment protocols/guidelines available. The clinician should be aware of the outcome of these treatments. Hence, the aim of this narrative review is to provide the background, clinical procedures and outcomes of surgical extrusion, intentional replantation and tooth autotransplantation.

Exploration for Suitable Vessels for Replantation in “The Middle-type” Fingertip Amputation in Early Childhood; A Follow-up to “Validity of Exploration for Suitable Vessels for Replantation in the Distal Fingertip Amputation in Early Childhood: Replantation or Composite Graft”

Introduction: Pediatric digital amputations are rare compared to adult cases. Although the indications for digital replantation in children are generally more liberal and aggressive, they become rather ambiguous when fingertips are involved, especially those of the fingertip at the level of mid nail bed (middle-type). Previously, we reported that exploration in pediatric (6-year-old and younger) middle-type fingertip amputation found suitable vessels for replantation in 50% of times but the result of subsequent replantation was always successful. Moreover, we experienced an overall success rate of 67% in exploration cases including those of subsequent replantation with suitable vessels and composite grafting without suitable vessels. This was higher than a success rate of 57% in cases treated with primary composite grafting without exploration. Recently, we have encountered four additional cases of pediatric middle-type fingertip amputation. Therefore, we reviewed and re-analyzed all our cases to verify the value of exploring suitable vessels for digital replantation over primary composite grafting, especially in early childhood, as a follow-up to our previous report. Method: A retrospective review of cases involving the surgical treatment of young children (6 years or younger), with single-digit complete middle-type fingertip amputations from 1993 to 2016 was conducted. Cases were divided into two groups: cases underwent reattachment as composite graft primarily and cases underwent exploration for suitable vessels for replantation. In the latter group when suitable vessels for replantation were found, replantation was attempted. On the other hand, when no suitable vessels were found, amputated parts were treated as secondary composite graft with or without pocket method. The success of replantation or reattachment as a composite graft was judged on the basis of the clinical records. Results: A total of 17 consecutive cases were identified, including 13 cases that were previously reported and four new cases. Of the four new cases, replantation was successful in three cases, while simple composite grafting failed after exploration in the other case. The probability to find suitable vessels increased to 60% with the addition of three successful replantations. However, the overall success rate for vessel exploration (70%) did not show statistical significance compared to that of primary composite grafting (57%). Conclusion: We, again recommend exploration for amputations at this level with a view to replantation. Where microsurgical replantation is not possible, the use of the pocket technique can lead to a higher chance of graft survival.

ОСОБЕННОСТИ РЕГЕНЕРАЦИИ КОСТНОЙ ТКАНИ В ПЕРИАПИКАЛЬНОЙ ЗОНЕ У РЕПЛАНТИРОВАННЫХ ЗУБОВ

Subject. In this article, the problem of integrating the repaid teeth has been studied. Data on the fibroosteointegration of the replanted teeth were obtained on the basis of clinical and additional research methods.
 Goal. To study the features of the regeneration of bone tissue in the periapical zone of the implantable teeth.
 Methodology. The patient, C., 41, complained of a partial absence of teeth. As a result of examination of the mouth and analysis of the orthopantomogram, significant destruction of the crown part of the teeth of the upper and lower jaws was revealed. Based on the data obtained, a decision was made to remove them with subsequent replantation. Three months after the implantation, an orthopantomogram was performed. According to the data of roentgenography, uniform filling of the root canal with cement along the entire length was revealed, trabecular bone tissue in the region of the tips of the roots of the resplanted teeth is consistent. With the support of the implantable teeth, non-removable cermet structures were installed. Later, prosthetics was performed with removable orthopedic prostheses.
 Results. After 2 years as a result of a follow-up examination, there was no pathological mobility in the resplanted teeth, the patient did not present any complaints. On intraoral virological images of the teeth 1.1, 1.2, 1.3, 3.4, the trabecular bone tissue in the projection of the apex of the roots is consistent, the inflammatory foci are not revealed. On the whole surface of the roots of the teeth 1.1-1.3 periodontal space can be traced throughout the root. Bone tissue in the region of the roots of these teeth without pathology. This observation may allow us to make an assumption about fibroosteointegration of these teeth.
 Conclusions. Based on the results of the treatment and analysis of X-ray data from two-year follow-up, we found no abnormal mobility in the resplanted teeth, bone tissue in the apex of these teeth is well-founded, orthopedic structures supported by the resplanted teeth are stable.

Late hand replantation with restoration of intrinsic muscle function: a case and review of the literature

Limb amputation injuries and their subsequent replantation is a time-critical exercise if function is to be restored. The exact time limit to functional replantation is still poorly defined in the literature owing to the multitude of factors that influence functional recovery. The relative metabolic activity of a tissue is lower under hypothermic conditions, and subsequently, moderate delays in restoring circulation can still achieve good functional outcomes if the amputated part is transported appropriately. Some surgeons have adopted the practice of, in cases where the delay to replantation is beyond 8–10 h, resecting skeletal muscle from the amputated part to avoid the sequelae of its ischaemic period. We report the case of a traumatic trans-carpal hand amputation in a 40-year-old male, with a second-level injury more distally at the metacarpal heads, who was replanted with a total ischaemic time of 19 h and 25 min. The decision was made to retain his intrinsic hand muscles in contradiction to common practice. At 2.5 years follow-up, he has regained a remarkable level of function of his hand including activation of his intrinsic hand muscles. His Semmes Weinstein testing has also shown good sensory recovery of his repaired nerves and he enjoys little, if any, disability as a result of his injury (despite a double-level injury on the dorsal ulna aspect of his forearm). This suggests, in cases of hand amputation with appropriate hypothermic transportation of the amputated part, ischaemia times up to 20 h may be compatible with restoration of intrinsic hand function. Level of Evidence: Level V, risk / prognostic study.

Analysis of Critically Perfused Tissues by Laser Speckle Contrast Analysis (LASCA) Perfusion Imaging

Background: The success of tissue transplantation and long-term tissue stability after wound healing depends on sufficient tissue perfusion. Laser Doppler-supported procedures allow for an objective measurement of relative tissue perfusion. The development of Speckle-based Laser Doppler imaging now enables a real-time representation of tissue perfusion. The perfusion of tissues relevant in plastic surgery such as scars, phalangeal replantations and burn wounds were systematically analysed by Laser Speckle Contrast Analysis (LASCA) imaging. Method: Perfusion of skin, scars, replanted fingers and different burn wound degrees (IIa, IIb and III) were systematically examined by LASCA imaging. Baseline perfusion of control tissue and perfusion values of compromised tissues were quantified and compared. Results: LASCA imaging shows significant differences in baseline perfusion of skin compared to atrophic scars, hypertrophic scars and keloids. Finger replantations with subsequent replantation failure show a characteristic and significant hypoperfusion in line with the expected clinical feature. A significant difference in tissue perfusion is seen in superficial (IIa) and superficial deep (IIb) burns, which are hard to distinguish on clinical examination. Deep burns (III) are characterised by hypoperfusion, which differs significantly from grade IIa and IIb burn wound perfusion, but not from baseline skin perfusion. Furthermore, the characteristic perfusion values of different burn degrees correspond to the varying treatment strategies. Conclusion: LASCA imaging is a robust method of perfusion imaging, which combines high resolution and speed. Hypoperfusion of tissues relevant to plastic surgery such as scars, phalangeal replantations and the different burn degrees (IIa, IIb and III) can be detected by LASCA imaging with high precision. Ease of use and immediate real-time imaging make LASCA imaging a reliable tool for the evaluation of tissue perfusion after plastic surgery procedures, which may influence further treatment decisions.

Penile amputation and successful reattachment and the role of winter shunt in postoperative viability: A case report and literature review

Traumatic self-amputation of the penis by a psychotic patient is rare. Microvascular replantation is the favored management approach. There are no known cases of self-amputation followed by ingestion of the stump and subsequent replantation. A 51-year-old patient with paranoid schizophrenia presented 2 hours following penile amputation. He had swallowed the excised portion, which was endoscopically retrieved from the stomach in the emergency department. Successful reattachment was achieved including microvascular repair of the dorsal penile arteries without cavernosal arterial anastamoses. A Winter's shunt was performed to improve venous circulation. The patient has been followed for 3 years from the date of repair. He has adequate erection for intercourse and good urinary function, but has experienced sensory loss over the dorsal aspect and glans and urethral stricture dilation. This is the first report of replantation following ingestion of an amputated penis.

Contradictions in the treatment of traumatic dental injuries and ways to proceed in dental trauma research*

Almost all treatment procedures used for dental traumas are still today not evidence-based, a fact, which makes it difficult to analyse the long-term outcome of healing and its relationship to treatment. Crown fractures with extensive dentin exposure represent a dominant injury in the permanent dentition. Accepted treatment philosophy is dentin coverage (dental liner and/or dentin bonded restoration) to prevent bacteria penetration into the pulp. Today there is, apart from deep proximal fractures, no evidence that this treatment is necessary to protect the pulp. In case of luxation injuries, the accepted treatment principles appear to be anatomically correct repositioning, stabilization with a splint and sometimes antibiotic coverage. In clinical studies, these principles could not be proven to optimize either periodontal or pulpal healing, the explanation possibly being that both reposition and application of splints in certain cases add extra damage to the pulp and periodontal ligament. In case of root fractures with dislocation, fast and optimal repositioning and rigid long-term splinting (i.e. 3 months) have been considered the principle of treatment. However, a recent clinical study has shown that short-term splinting with a semi-rigid splint appears to optimize fracture healing. In tooth avulsion with subsequent replantation, cleansing of the root surface for contamination and systemic antibiotics has been considered essential for pulp and periodontal healing. These treatment concepts have been derived from experimental studies in animals. However, their importance could not be verified in large clinical studies. Ideally, randomized clinical studies are needed in the future for selected trauma types. The influences of repositioning, splinting and the role of infection and antibiotics should be further investigated. However, for ethical reasons, it will be difficult to perform randomized studies on trauma victims and we will be forced in the future to rely on experimental animal studies supported by clinical observational studies.

Delayed, bilateral, non-microvascular ear replantation after violent amputation

Amputation of any body part is undoubtedly a traumatic experience leaving a terrible deformity, especially when the part or parts involved are visible and constitute an essential component of someone's facial whole. Bilateral ear amputation and successful subsequent replantation has been reported historically, but not in the modern surgical literature. We report the case of a 27-year-old female who was abducted and suffered a bilateral ear amputation at the hands of one of her captors to speed delivery of ransom money; the severed parts were sent to the parents approximately 2 hours after the amputation had taken place, and the girl was released some 48 hours after the ears were delivered. Microvascular replantation was attempted immediately after admission to the hospital some 2 hours after her release, but failed, and so a non-microvascular replantation was performed and was successful, after approximately 54 hours of ischaemia time. We consider this the first report of a complete bilateral, delayed, non-microvascular, successful ear replantation in a human being in the modern literature.

Treatment of avulsed teeth with Emdogain®– a case report

The present case report describes the reimplantation of avulsed teeth with the treatment of Emdogain. Case was avulsed right maxillary permanent central and lateral incisor in a 9-year-old girl suffering from a traumatic injury. After pretreatment of avulsed teeth, Emdogain was applied to the root surface and into the extraction socket with subsequent replantation of the tooth. Evaluation parameters included horizontal and vertical percussion sound and periapical radiographs. At 1-2-6-12-month follow-up period, the clinical and radiographic appearance of the teeth showed resolution of mobility and no signs of replacement resorbption.

Treatment of replacement resorption with Emdogain--preliminary results after 10 months.

Ankylosis of traumatized teeth in children and adolescents may inhibit further development and growth of the corresponding jawbone. Therefore, ankylosed teeth should be removed. As an alternative treatment option to autotransplantation of a premolar, intentional replantation using Emdogain may be considered, provided the ankylosis is detected at an early stage or has only affected a small area of the root. Eleven ankylosed teeth presenting with replacement resorption were treated as follows: after tooth extraction, the root canal was treated extraorally and obturated by retrograde insertion of a titanium post. Emdogain was applied to the root surface and into the extraction socket with subsequent replantation of the tooth. During a mean follow-up period of 6.3 months, no signs of recurrence of ankylosis were noted. The horizontally and vertically measured Periotest scores were identical to those obtained on the adjacent teeth. These results suggest that intentional replantation using Emdogain may prevent or delay ankylosis of these replanted teeth.

Microvascular transplantation of the rat submandibular gland.

Xerostomia results from salivary gland irradiation during treatment of head and neck malignancies. In addition to having difficulty with speech and swallowing, these patients experience loss of taste, dental caries, and chronic fungal infections. The paired submandibular glands provide 70 percent of the normal salivary flow and are difficult to shield during radiation therapy. Another sicca condition, xerophthalmia, may result from facial nerve injury or other medical disorders and results in pain, corneal ulceration, and possible vision loss. Treatment options for xerostomia are limited, and management of xerophthalmia usually focuses on the eyelids, rather than the fundamental problem of inadequate secretory protection. In this study, a rat model for submandibular gland microvascular transplantation was developed to assess the feasibility of salivary tissue transfer. Sixteen rats underwent submandibular gland transplantation from the neck to the groin. Fourteen of these rats underwent microvascular anastomosis of the vascular pedicle. Ten glands were assessed for viability at 4 days after transplantation, and four glands were examined after 7, 10, 14, or 21 days. By gross and histologic examination, 93 percent of transplanted glands showed expected long-term viability after at least 4 postoperative days. Microvascular techniques were shown to be applicable to the transplantation of submandibular gland salivary tissue. This has not previously been shown in a rat model. It is possible that submandibular glands could be transplanted to the eye for treatment of xerophthalmia and out of the neck during irradiation of the head and neck, with subsequent replantation after treatment as a means of preventing permanent xerostomia.

Viability of skin subjected to deep partial skin thickness thermal damage: experimental studies

Secondary tissue loss in burn wounds (due to necrosis in the zone of stasis) is interpreted as a sequel of progressive vascular occulsion and dehydration of thermally damaged tissue. In this study on rats, delayed healing represented an additional factor in determining the fate of tissue that had sustained deep partial skin thickness burns. Early revascularization induced by excision and subsequent replantation of the burned skin resulted in survival of a large part of the corium. Replantation reduced the loss of full thickness skin seen in controls left to heal spontaneously to partial loss in treated wounds. The experiments substantiate the theory that deep partial skin thickness thermal damage is at least partly reversible and indicate that with tangential excision of dermal burns a viable part of the corium is sacrificed. This deeper part of the corium can be saved by early excision and replantation.

Temporary ectopic implantation

Temporary ectopic implantation is a technique designed for extremity salvage in cases of devascularization or amputation of an essentially intact part in conjunction with extensive segmental proximal injury. A case of a traumatic midforearm amputation that survived by ectopic implantation in the abdominal wall, with subsequent replantation eleven weeks later, is presented.