Strong Antioxidant Activity Research Articles

The antioxidant activity of shaddock peel polysaccharides

The shaddock peel polysaccharide (SPP) has various biological activities. This explored its antioxidant activity. In vitro antioxidant tests, the two SPP components showed good DPPH, hydroxyl radical scavenging ability and anti-lipid peroxidation ability. At a concentration of 3.2 mg/mL, the anti-lipid peroxidation abilities of the two polysaccharides were 21.65% and 18.96%, respectively. SPP-3 had a slightly stronger ability to scavenge hydroxyl radicals than SPP-2. When the polysaccharide concentration reached 3.2 mg/mL, the order of the two polysaccharides’ DPPH scavenging rates was SPP-2 > SPP-3. Therefore, SPP showed strong antioxidant activity and could be used in antioxidant-related applications in the future.

Exploring the antibacterial efficacy of Opuntia monacantha in combatting methicillin-resistant Staphylococcus aureus

Public health is seriously threatened by the rise of antibiotic-resistant strains of bacteria, especially methicillin resistant Staphylococcus aureus (MRSA). This study investigated the phytochemical compounds, and possible antibacterial effects of a methanolic extract of the cactus species Opuntia monacantha Haw. against MRSA. The powdered substance was extracted with methanol and filtered, and the filtrate was partitioned with n-hexane, chloroform and ethyl acetate. The fractions with higher percentage yields such as the n-hexane and chloroform fractions went through GC-MS analysis. They consist of various compounds that are known to have strong antioxidant and antimicrobial activities. The antimicrobial activity of the plant was measured via a diffusion assay. On the basis of these results, the Opuntia monacantha crude methanolic extract, and the n-hexane and chloroform fractions significantly inhibited all the MRSA strains used in the test at 100, 75 and 50 mg/mL. Furthermore, the minimum inhibitory concentrations of various fractions of the methanolic extract of Opuntia monacantha were also examined. Therefore, Opuntia could be a promising candidate for strengthening the existing formulations in pharmaceutical science for the treatment of MRSA. However, additional research is necessary before its therapeutic use is recommended. The results are intended to provide important new information for the creation of sustainable and alternative antimicrobial agents.

Astaxanthin: Unveiling biochemical mysteries, expanding horizons, and therapeutic opportunities in health science and biomedical research

This systemic study surveys the multifaceted nature of Astaxanthin (AXT), a member of carotenoid pigments broadly used in nutraceuticals and pharmaceuticals. Starting with an insight into its biological origin, the review proceeds to detail the complex chemical structure of AXT followed by considerations on its bioavailability, pharmacokinetics and safety as a dietary supplement. Foremost among these is the biological activities of AXT, especially its strong antioxidant activity which plays an important role in reducing oxidative stress (OS) damage to cells. The description of AXT as an anti-apoptotic and anti-inflammatory cytokine indicates its important role in cell protection and chronic inflammation improvement. Additional studies emphasize positive anti-obesity and anti-diabetic activities that could be exploited as therapy for metabolic disease. The review goes on to describe the immunomodulatory and neuroprotective effects of AXT, its role in cardiovascular protection, as well as hepatic health. The discussion of the anti-cancer activity of AXT is important, since it is related with its mechanisms for preventing and treating cancer. The broad perspective ends with an overview of the diverse biological activities of AXT, suggesting future research directions and its ability to be a multi-target ameliorator. Data compiled here aims to significantly help to improve knowledge on AXT, thus facilitating health and biomedical research progression.

Anti-glycation activity and mechanism of Siraitia grosvenorii polysaccharide based on bovine serum albumin-fructose and Caco-2 cell models.

Anti-glycation activity and mechanism of Siraitia grosvenorii polysaccharide based on bovine serum albumin-fructose and Caco-2 cell models.

Use of Nigella sativa silver nanocomposite as an alternative therapy against thioacetamide nephrotoxicity

Nigella sativa (N. sativa) L. (Ranunculaceae), commonly referred to as black cumin, has a long history of usage as an herbal remedy. It has been utilized conventionally and in clinical settings to treat various illnesses. Six groups of male Wister rats were randomly selected as Gp I, represented as control; Gp II administered N. sativa aqueous extract (NSAE); 200 mg/kg/d, Gp III received N. sativa silver nanocomposite (NS-Ag-NC); 0.25 mg/kg/d; Gp IV administered thioacetamide (TAA);100 mg/kg; thrice weekly and Gps V and VI administered NSAE and NS-Ag-NC with TAA for six weeks, respectively. Findings showed that GC-MS analysis of NSAE has a high concentration of phytochemicals with strong antioxidant activity. Results revealed that TAA administration elevated TBARS, H2O2, PCC, NO levels, kidney function parameters, LDH activity, and up-regulated TNF-α, IL-1β, NF-kβ, and COX-2 gene expressions. In contrast, enzymatic and non-enzymatic antioxidants and ALP activity were extensively diminished. Also, severe abnormalities in lipid profile, hematological parameters, and histopathological features were noted. On the other hand, the administration of NSAE or NS-Ag-NC followed by TAA intoxication reduces renal impairment, restores the antioxidant system, and downregulates the expression of TNF-α, IL-1β, NF-kβ, and COX-2 genes in rats’ renal tissues. Collectively, NS-Ag-NC has more prevalent nephroprotective impacts than NSAE and can adjust the oxidant/antioxidant pathways besides their anti-inflammatory efficacy against TAA toxicity.

Brilliant fabrication of multifunctional collagen-based packaging films with rigid-flexible coupling structure bio-inspired by pangolins.

Brilliant fabrication of multifunctional collagen-based packaging films with rigid-flexible coupling structure bio-inspired by pangolins.

Ethanolic extract of Cnidoscolus aconitifolius leaves alleviates insulin resistance by modulating oxidative stress and inflammation in albino rats exposed to heavy metal mixture

Exposure to heavy metals significantly contributes to insulin resistance, a major factor in type 2 diabetes. This study investigated the antioxidant and therapeutic potential of ethanol leaf extract of Cnidoscolus aconitifolius in mitigating heavy metal-induced insulin resistance, oxidative stress and inflammation in albino rats. Thirty rats were divided into five groups: Groups I and II received normal saline and a lead-mercury mixture, respectively, while Groups III, IV and V were treated with the extract (200, 400 and 600 mg/kg) for four weeks after exposure. The extract, rich in catechin, rutin, gallic acid and kaempferol, exhibited strong antioxidant activity. Heavy metal exposure induced hepatic insulin resistance, dyslipidaemia, oxidative stress and inflammation, as shown by elevated HOMA-IR values. Extract treatment reversed these effects in a dose-dependent manner, restoring insulin sensitivity via oxidative stress and inflammation modulation. This highlights the potential of C. aconitifolius as a nutraceutical for heavy metal-induced metabolic disorders.

Phytochemical analysis of Anacamptis sancta: Glucomannan, phenolic components, and antioxidant activity

Background and purpose: This study investigated the extracts obtained from both the roots and above-ground parts of Anacamptis sancta. A. sancta, an orchid species traditionally used for its potential health benefits due to its rich phytochemical composition, was the focus. The primary aim of this research was to analyze the phytochemical components of A. sancta extracts and evaluate their antioxidant potential. By conducting comprehensive phytochemical analyses and assessing antioxidant activities, this study seeks to provide a deeper understanding of the medicinal properties of A. sancta and contribute to its applications in functional foods and pharmaceuticals. Materials and methods: In this study, extracts derived from both the tubers and above-ground parts of A. sancta were investigated. Plant samples were collected from the Ondokuz Mayıs University Botanical Garden. Fourier transform infrared spectroscopy (FTIR) analysis was conducted using a Perkin Elmer Spectrum 400 spectrophotometer. Glucomannan content was measured calorimetrically using the Megazyme K-GLUM 03/20 analysis kit and UV-visible spectrophotometer. Analysis of secondary metabolites in the above-ground parts included determination of total phenolic, flavonoid, flavanol, tannin, and proanthocyanidin contents using Folin-Ciocalteu, AlCl3, and other appropriate methods. Antioxidant capacity was assessed through the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and compound identification in the above-ground parts was performed using gas chromatography/ mass spectrometry (GC-MS) analysis. Results: In the tubers, a glucomannan content of 17.93% was detected, and FTIR analyses revealed the characteristic peaks of glucomannan. The extracts demonstrated high antioxidant potential, with total phenolic content measured at 120.61 mg GAE/g extract, total flavonoid content at 295.46 mg QE/g extract, flavanol content at 32.30 mg QE/g extract, total tannin content at 3.05 mg GAE/g extract, and total proanthocyanidin content at 119.43 mg CAE/g extract. The IC50 value for DPPH radical scavenging activity was calculated to be 47.79 mg/mL. GC-MS analysis of the above-ground parts of the plant identified the presence of significant bioactive compounds, including hydroxyacetic acid, hydrazide (42.52%), propane, 1,1-dipropoxy- (16.94%), and 1,3-dioxolane-4-methanol, 2-ethyl (12.86%). In summary, this study presents a comprehensive analysis of the phytochemical profile of A. sancta. Conclusions: The findings highlight its significant glucomannan content in tubers and strong antioxidant activity. Additionally, bioactive compounds were identified in the above-ground parts. This research provides valuable insights into potential medicinal and industrial applications of A. sancta.

Unveiling the geroprotective potential of Monarda didyma L.: insights from in vitro studies and a randomized clinical trial on slowing biological aging and improving quality of life

Abstract Aging is driven by fundamental mechanisms like oxidative stress, telomere shortening and changes in DNA methylation, which together prepare the ground for age-related diseases. Botanical extracts, rich in bioactive phytoconstituents, represent a promising resource for developing therapies that target these mechanisms to promote healthy aging. This study explores the geroprotective potential of Monarda didyma L. extract. In vitro analyses revealed the extract’s strong antioxidant activity, ability to reduce telomere shortening, and capacity to protect against DNA damage, thereby decreasing cellular senescence and improving endothelial function. The randomized, double-blind clinical trial demonstrated that daily oral supplementation with the extract significantly improved leukocyte telomere length (LTL) and stabilized DNA methylation age (DNAmAge) in the intervention group, while the placebo group experienced accelerated epigenetic aging and hypermethylation of critical age-related genes (ELOVL2 and FHL2). The intervention group also reported enhanced quality of life, particularly in the physical domain, along with improved movement and quality sleep indices detected by questionnaire and wearable sensors. These compelling findings position Monarda didyma L. extract as a powerful candidate for future geroprotective therapies, with the potential to significantly impact healthy aging.

Chemical components, health-promoting effects and industrial application of a Chinese bitter tea (Kuding tea): A comprehensive review.

Chemical components, health-promoting effects and industrial application of a Chinese bitter tea (Kuding tea): A comprehensive review.

Dynamics Changes in Physicochemical Properties, Antioxidant Activity, and Non-Volatile Metabolites During Bulang Pickled Tea Fermentation

The present study investigated the dynamics changes in physicochemical properties and non-volatile metabolites during Bulang pickled tea fermentation. A combination of artificial sensory evaluation, chemical-physical analysis, ultra performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS), and multivariate statistical analysis were employed to examine the differences among four fermentation stages of Bulang pickled tea. The bitterness, astringency, sweetness after taste, sourness and fermentation taste tended to increase with fermentation. The highest lactic acid bacteria, aerobic bacteria, total titratable acidity, total soluble sugar, total polyphenols, and total flavonoids were recorded at the second month of fermentation, while fungi, total free amino acids, total antioxidant capacity and hydroxyl free radical scavenging capacity increased with fermentation. Mantel test demonstrated significant associations between lactic acid bacteria /fungal communities and taste characteristics. UPLC-QTOF-MS analysis led to the identification of 35 differential non-volatile metabolites, predominantly comprising heterocyclic compounds, organic acids with their derivatives, and flavonoids. Nine non-volatile metabolites are related to antioxidant activity, and morin, malvidin and 7-methylxanthine exhibit relatively strong antioxidant activity. This study provides comprehensive insights into the non-volatile metabolites and antioxidant function of Bulang pickled tea.

Recent advances in the multifaceted mechanisms of catalpol in treating osteoporosis.

Catalpol (CAT) is a landmark active ingredient in traditional Chinese medicine Rehmannia (TCT), also known as dehydroxybenzoate catalpone, which is a kind of iridoid terpene glycoside with strong antioxidant, anti-inflammatory, antitumor and other biological activities. It can exert its anti-disease effect in a variety of ways. For some patients with chronic diseases, the application of azalea alcohol in rehmannia may bring more comprehensive and long-lasting efficacy. Studies have shown that the anti-disease effect of catalpol in osteoporosis (OP) is mainly achieved through various pathways such as Wnt/β-catenin signaling pathways to promote osteogenic differentiation, and RANKL/RANK and other signaling pathways to inhibit osteoclastic differentiation. At present, there is a slight lack of analysis of the mechanism of action of catalpa alcohol in the treatment of osteoporosis, so this study comprehensively searched the literature on the mechanism of action of catalpa alcohol in the treatment of osteoporosis in various databases, and reviewed the research progress of its role and mechanism, to provide reference and theoretical basis for the further development and application of catalpol.

Bioactivity of Songga Wood (Strychnos ligustrina) methanol extract: Antioxidant and Anti-inflammatory against Interleukin-8 (Il-8) levels in Vivo

Songga wood (Strychnos ligustrina) from the genus Loganiaceae is a plant that is empirically used as a traditional medicine by people in several regions in Indonesia. This plant is used as a medicine for fever, malaria, antidote, wounds, boils, acne and scabies. This study aims to determine the antioxidant and anti-inflammatory activity of methanol extract of songga wood with Interleukin-8 (IL-8) parameter. Songga wood was macerated with methanol for 3x24 hours until a thick extract was obtained. Antioxidant activity test was conducted by DPPH method and anti-inflammatory by ELISA with IL-8 parameter. Test animals were divided into six groups, namely the normal group, negative control (Na-CMC), positive control (diclofenac sodium) and treatment with three dose variations, namely 50, 100, and 200 mg/KgBB. The results showed that the antioxidant activity value of Songga wood was IC50 84.51 μg/ml. Furthermore, anti-inflammatory activity showed IL-8 levels at doses of 50 mg/kgBB extract (494.8 pg/ml), 100 mg/kgBB (216.2 pg/ml) and 200 mg/kgBB (118.7 pg/ml). This study concluded that methanol extract of songga wood has strong antioxidant activity and has activity as an anti-inflammatory and the most effective dose of IL-8 levels is 200 mg/kgBB (p>0.05).

Green synthesis of silver nanoparticles from the endophytic fungus Panax notoginseng and their antioxidant and antimicrobial activities and effects on cherry tomato preservation.

Green synthesis of silver nanoparticles from the endophytic fungus Panax notoginseng and their antioxidant and antimicrobial activities and effects on cherry tomato preservation.

Phytochemicals in pomegranate peel extract attenuate oxidative damage in rats exposed to fipronil.

Phytochemicals in pomegranate peel extract attenuate oxidative damage in rats exposed to fipronil.

Fabrication of SA/CMC composite membrane embedded with ascorbic acid and cross-linked through propylene glycol: The study on the preservation effects on fresh-cut eggplants.

In this study, sodium alginate/sodium hydroxymethyl cellulose (SA/CMC)-based biodegradable membranes cross-linked with propylene glycol (PG) and incorporated with ascorbic acid (AA) were synthesized. The color, morphology, water vapor permeability (WVP), and antioxidant capacity of every kind of membrane were investigated. The morphological characterization affirmed that the resultant blend membranes exhibited a well-organized homogeneous structure without cracks. Moreover, the antioxidant capacity increased with increasing concentration of AA in the obtained membranes. The increase in the concentration of AA affected the WVP of the blended membranes. The weight loss rate, total soluble solid, total phenol, and MDA content of the SA/CMC/PG/1.0% (v/w) AA experimental groups were 15±0.02%, 4.891±0.04%, 0.59±0.05mgg-1, and 0.174µmolg-1, respectively. Hence, the release and DPPH radical scavenging assay of incorporating AA in SA/CMC/PG membrane showed that the obtained SA/CMC/PG membrane with 1.0% AA can release AA slowly and have strong antioxidant activity. Thus, 1.0% AA incorporated into SA/CMC/PG membrane was an effective method to establish an active and environmentally friendly food packing material. PRACTICAL APPLICATION: We established sodium alginate/sodium hydroxymethyl cellulose (SA/CMC)-based biodegradable membranes incorporated with ascorbic acid (AA) and cross-linked with propylene glycol (PG). After being characterized, the SA, SA/CMC, SA/CMC/PG, 0.2% (v/w) AA/SA/CMC/PG, and 1.0% (v/w) AA/SA/CMC/PG membrane were applied to preserve the fresh-cut eggplants. Though the determination of the total soluble solid, total phenol content, and malondialdehyde content in purple eggplant during the 8 days of storage, the result shows that the obtained sodium alginate/sodium hydroxymethyl cellulose/propylene glycol/1% (v/w) AA composite membranes have a potential application in active food packaging.

Preparation of a biodegradable packaging film by konjac glucomannan/sodium alginate reinforced with nitrogen-doped carbon quantum dots from crayfish shell for crayfish meat preservation.

Preparation of a biodegradable packaging film by konjac glucomannan/sodium alginate reinforced with nitrogen-doped carbon quantum dots from crayfish shell for crayfish meat preservation.

Metallofullerenol Gd@C82(OH)22 preserves human erythrocyte plasma membrane integrity from AAPH-induced oxidative stress: Molecular mechanisms and antioxidant activity.

Metallofullerenol Gd@C82(OH)22 preserves human erythrocyte plasma membrane integrity from AAPH-induced oxidative stress: Molecular mechanisms and antioxidant activity.

Microwave-assisted protein extraction from foxtail millet: Optimization, structural characterization, techno-functional properties, and bioactivity of peptides.

Microwave-assisted protein extraction from foxtail millet: Optimization, structural characterization, techno-functional properties, and bioactivity of peptides.

Mitigation of gentamycin induced acute kidney injury due to benzothiazole derivatives N1 and N5: Antioxidant and renoprotective mechanisms in-vivo zebrafish.

Mitigation of gentamycin induced acute kidney injury due to benzothiazole derivatives N1 and N5: Antioxidant and renoprotective mechanisms in-vivo zebrafish.