Herein, we obtained heterogeneous silver nanoparticles from the isolated strain Streptomyces pactum and characterized them. These nanoparticles were utilized as recyclable green catalysts in a one-pot, three-component reaction to synthesize fluorinated and thiazolidine-4-one derivatives (Figure 1). Dimethylformamide (DMF) was identified as a suitable solvent, and the impact of the biocatalyst on product yields was effectively observed.

Wheat (Triticum aestivum L.) is a globally important staple crop; however, its growth and yield are severely limited by drought stress. This study evaluated the effects of a combined microbial inoculant, Streptomyces pactum Act12 and Streptomyces rochei D74, on wheat photosynthesis, physiological traits, and yield under drought conditions. Key physiological and yield parameters were measured during the jointing, heading, and grain-filling stages. Drought stress significantly reduced chlorophyll content, maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm), and antioxidant enzyme activities, while increasing malondialdehyde (MDA) levels, leading to a notable yield decline. In contrast, inoculation with Streptomyces strains alleviated these adverse effects, with the combined inoculant (Act12+D74) group demonstrating the most significant improvement. Chlorophyll content increased by up to 32.60%, Fv/Fm improved by 43.07%, and antioxidant enzyme activities were enhanced, with superoxide dismutase (SOD) activity increasing by 19.32% and peroxidase (POD) activity by 75.44%. Meanwhile, MDA levels were reduced by 61.61%. The proline content in the combined inoculant group increased by 90.44% at the jointing stage and the soluble protein content increased by 60.17% at the heading stage. Furthermore, it improved the yield by 26.19% by increasing both effective spikes and grains per spike. For the first time, this study revealed the synergistic effects of Act12 and D74 in enhancing photosynthesis, strengthening antioxidant defenses, and optimizing osmotic regulation under drought conditions. These findings provide a theoretical basis for developing environmentally friendly drought management strategies and highlight the potential applications of this inoculant in sustainable agriculture.

Annually, a significant volume of chemicals, encompassing fertilizers and pesticides, is administered to agricultural soils. Using pesticides and fertilizers, agricultural practices contribute to heavy metal Cadmium (Cd), Copper (Cu), Lead (Pb) and Zinc (Zn) pollution. Heavy metals and pesticides are high at the peak of ecological contaminants, presence of this has introduced grave risks to the health of the population and agronomics. Among heavy metals, cadmium (Cd) is recognized for its high mobility in various environmental settings. Cd has a deleterious effect on plant phenotypic, cytotoxicity (e.g., lowering chlorophyll concentration and limiting photosynthetic effectiveness), and metabolic activities (e.g., chlorosis and necrosis). Microbial bioremediation by using microorganisms is one of the secure, pure, cost operative and eco-friendly technology for decontaminating polluting sites as compared to physical and chemical techniques. Among microbes, Actinobacteria hold a paramount position, serving as key players in numerous biological processes, utilize toxins as carbon source and turn into high concentrations of pesticides, chemical complexes and heavy metals into commercially viable antibiotics, enzymes, proteins, and plant growth promoting hormones. This study is an effort to explore the potent cadmium resistance actinomycetes to reduce cadmium levels to enhance degradation. For this purpose, 53 actinomycetes strains were tested for heavy metal resistance and tolerance to Cadmium against different concentrations. After secondary screening Four potent isolate have the potential to grow at 1000 mg/L concentration of Cadmium in the medium. When they are able to grow on heavy metal containing media it could be beneficial for reduction and elimination of toxic metals from contaminated environment. When it comes to achieving a suitable level of metal tolerance, this potent powerful actinomycetes strain Streptomyces pactum have been identified to be promising.

Cadmium is a dangerous environmental contaminant that disturbs numerous ecological and environmental systems while being poisonous to plants and the microbial life. The present research attempted to utilize previously isolated Streptomyces pactum OR958669 from polluted soil to promote plant development and prevent cadmium's detrimental impacts. Streptomyces pactum OR958669 was chosen for this because it was the highly Cd-tolerant isolate and produced a significant amount of siderophore (43.94%). It was isolated from agricultural soil that had been polluted by pesticides. The impact of Cd (1000ppm) and Streptomyces pactum OR958669 on some physiological traits of the Groundnut plants grown for 10 weeks were determined. Plant’s root and shoot development (length, fresh and dry masses), number of leaves, N, P, K, and Mg concentrations all diminished when they were irrigated with water that included heavy metals. Heavy metals reduced the amount of chlorophyll (Chl) in the leaves and the number of soluble proteins in the shoots, but it raised the amounts of total soluble carbohydrates and proline. Utilizing Streptomyces pactum OR958669 to the test soil increased the plant's resistance to heavy metals, which had an impact on the majority of the test characteristics. In order to increase agricultural productivity, it was determined that adding Streptomyces pactum OR958669 to the soil produced siderophores effectively and inexpensively.

Streptomyces pactum (Act12) can both promote plant growth and strengthen heavy metal mobilization. Nevertheless, the mechanisms of how Act12 works during the phytoextraction process are still unknown. The present work investigated whether the metabolites produced by Act12 could influence the seed germination and the growth of potherb mustard and explored its mobilizing effect on soil cadmium (Cd) and zinc (Zn). The results showed that the germination potential and rate of potherb mustard seed treated with Act12 fermentation broth were 1.0- and 0.32-folds higher than those of control, probably due to the interruption of seed dormant stage. We also found that Act12 inoculation not only promoted the dry biomass (6.82%) of potherb mustard, but also increased the leaf chlorophyll (11.8%) and soluble protein (0.35%) production. The boosted seed germination rate under Act12 treatment (up to 63.3%) indicated that Act12 enhanced the resistance of potherb mustard seeds to Cd and Zn and alleviated their physiological toxicity. The generated metabolites during the Act12 fermentation posed positive impact on the availability of soil Cd and Zn. These findings bring new insight into the Act12-assisted phytoextraction of Cd and Zn from contaminated soils.

The potent antitumor antibiotic pactamycin is an aminocyclopentitol-containing natural product produced by the soil bacterium Streptomyces pactum. Recent studies showed that the aminocyclopentitol unit is derived from N-acetyl-D-glucosamine, which is attached to an acyl carrier protein (ACP)-bound polyketide by a glycosyltransferase enzyme, PtmJ. Here, we report a series of post-glycosylation modifications of the sugar moiety of the glycosylated polyketide while it is still attached to the carrier protein. In vitro reconstitution of PtmS (an AMP-ligase), PtmI (an ACP), PtmJ, PtmN (an oxidoreductase), PtmA (an aminotransferase), and PtmB (a putative carbamoyltransferase) showed that the N-acetyl-D-glucosamine moiety of the glycosylated polyketide is first oxidized by PtmN and then transaminated by PtmA to give ACP-bound 3-amino-3-deoxy-N-acetyl-D-glucosaminyl polyketide. The amino group is then coupled with carbamoyl phosphate by PtmB to give a urea functionality. We also show that PtmG is a deacetylase that hydrolyses the C-2 N-acetyl group to give a free amine.

Widely targeted metabolomic, transcriptomic, and metagenomic profiling reveal microbe–plant–metabolic reprogramming patterns mediated by Streptomyces pactum Act12 enhance the fruit quality of Capsicum annuum L

The objective of this study was to isolate Streptomyces sp. from north paddy soils of Iran and investigation and identification of the bioactive compounds by carrying out GC-MS analysis. Antimicrobial activity was investigated by well diffusion agar technique against the pathogenic microorganisms including Enterococcus faecalis ATCC 29212, Micrococcus luteus ATCC 4698, Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 9144, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 43071. Based on the results of gene sequencing of gene 16S rRNA and phylogenetic analysis, the isolated sample belongs to the genus Streptomyces with the highest degree of resemblance (99/87%) to the Streptomyces pactum strain ACT12. The isolate showed a broad spectrum of antibacterial activity against test microorganisms. This isolate showed maximum antibacterial activity against M. luteus (25 ± 0.5 mm) and the most resistant microorganism against antibacterial activity of this isolate was P. aeruginosa (9 ± 0.5 mm). The contact bioautography method was used to detect compounds that were responsible for antimicrobial activity and showed the active compounds with Rf values of 0.8-0.9. The identification of bioactive metabolites were performed using gas chromatography-mass spectrometry (GC-MS). GC-MS analysis of the extract showed the presence of 15 volatile compounds. The main compounds were Methyl-3-(3,5-ditertbutyl-4-hydroxyphenyl) (10.88%) and Dibutyl phthalate (8.34%) in comparison with other bioactive compounds. The results showed that north paddy soils of Iran are a rich source of microbial flora for the production of antimicrobial compounds and useful for antimicrobial compounds discovery from Streptomyces sp.

The biosynthetic gene cluster of NFAT-133, an inhibitor of the nuclear factor of activated T cells, was recently identified in Streptomyces pactum ATCC 27456. This cluster is conspicuous by its highly disordered noncollinear type I modular polyketide synthase (PKS) genes that encode PKSs with one module more than those expected for the heptaketide NFAT-133 biosynthesis. Thus, the major metabolite NFAT-133 was proposed to derive from an octaketide analogue, TM-123. Here, we report that further bioinformatic analysis and gene inactivation studies suggest that NFAT-133 is not derived from TM-123 but rather a product of programmed KS7 extension skipping of a nascent heptaketide from the PKS assembly line that produces TM-123. Furthermore, identification of NFAT-133/TM-123 analogues from mutants of the ATCC 27456 strain suggests that NftN (a putative dehydrogenase), NftE (a cytochrome P450), and NftG (a putative hydrolase/decarboxylase) function "in trans" during the polyketide chain assembly processes.

Moss-dominated biocrusts (moss crusts) are a feasible approach for the ecological restoration of drylands, but difficulty obtaining inoculum severely limits the progress of large-scale field applications. Exogenous microorganisms could improve moss growth and be conducive to moss inoculum propagation. In this study, we investigated the growth-promoting effects and potential mechanisms of exogenous microorganism additives on moss crusts. We used an incubator study to examine the effects of inoculation by heterotrophic microorganisms (Streptomyces pactum, Bacillus megaterium) and autotrophic microorganisms (Chlorella vulgaris, Microcoleus vaginatus) combined with Artemisia sphaerocephala gum on the growth of Bryum argenteum, the dominant moss crusts species in sandy deserts. Amplicon sequencing (16S and 18S rRNA) and PICRUSt2 were used to illustrate the microbial community structure and potential function in the optimal treatment at different developmental stages. Our results showed that exogenous microorganisms significantly promoted moss growth and increased aboveground biomass. After 30 days of cultivation, the Streptomyces pactum (1 g kg–1 substrate) + Chlorella vulgaris (3.33 L m–2) treatment presented optimal moss coverage, height, and density of 97.14%, 28.31 mm, and 2.28 g cm–2, respectively. The best-performing treatment had a higher relative abundance of Streptophyta—involved in moss growth—than the control. The control had significantly higher soil organic carbon than the best-performing treatment on day 30. Exogenous microorganisms improved eukaryotic community diversity and richness and may enhance soil microbial functional and metabolic diversity, such as growth and reproduction, carbon fixation, and cellulose and lignin decomposition, based on functional predictions. In summary, we identified the growth-promoting mechanisms of exogenous additives, providing a valuable reference for optimizing propagation technology for moss inoculum.

Rhizobacteria helps to explain the enhanced efficiency of phytoextraction strengthened by Streptomyces pactum

Actinobacterial biofertilizer improves the yields of different plants and alters the assembly processes of rhizosphere microbial communities

Filamentous soil bacteria are known to produce diverse specialized metabolites. Despite having enormous potential as a source of pharmaceuticals, they often produce bioactive metabolites at low titers. Here, we show that inactivation of the pactamycin, NFAT-133, and conglobatin biosynthetic pathways in Streptomyces pactum ATCC 27456 significantly increases the production of the mitochondrial electron transport inhibitors piericidins. Similarly, inactivation of the pactamycin, NFAT-133, and piericidin pathways significantly increases the production of the heat-shock protein (Hsp) 90 inhibitor conglobatin. In addition, four new conglobatin analogues (B2, B3, F1, and F2) with altered polyketide backbones, together with the known analogue conglobatin B1, were identified in this mutant, indicating that the conglobatin biosynthetic machinery is promiscuous toward different substrates. Among the new conglobatin analogues, conglobatin F2 showed enhanced antitumor activity against HeLa and NCI-H460 cancer cell lines compared to conglobatin. Conglobatin F2 also inhibits colony formation of HeLa cells in a dose-dependent manner. Molecular modeling studies suggest that the new conglobatins bind to human Hsp90 and disrupt Hsp90/Cdc37 chaperone/co-chaperone interactions in the same manner as conglobatin. The study also showed that genes that are involved in piericidin biosynthesis are clustered in two different loci located distantly in the S. pactum genome.

Streptomyces pactum and Bacillus consortium influenced the bioavailability of toxic metals, soil health, and growth attributes of Symphytum officinale in smelter/mining polluted soil

Streptomyces pactum and sulfur mediated the rhizosphere microhabitats of potherb mustard after a phytoextraction trial.

Green remediation of toxic metals contaminated mining soil using bacterial consortium and Brassica juncea.

Nature has always been seemingly limitless in its ability to create new chemical entities. It provides vastly diverse natural compounds through a biomanufacturing process that involves myriads of biosynthetic machineries. Here we report a case of unusual formations of hybrid natural products that are derived from two distinct polyketide biosynthetic pathways, the NFAT-133 and conglobatin pathways, in Streptomyces pactum ATCC 27456. Their chemical structures were determined by NMR spectroscopy, mass spectrometry, and chemical synthesis. Genome sequence analysis and gene inactivation experiments uncovered the biosynthetic gene cluster of conglobatin in S. pactum. Biochemical studies of the recombinant thioesterase (TE) domain of the conglobatin polyketide synthase (PKS) as well as its S74A mutant revealed that the formation of these hybrid compounds requires an active TE domain. We propose that NFAT-133 can interfere with conglobatin biosynthesis by reacting with the TE-domain-bound intermediates in the conglobatin PKS assembly line to form hybrid NFAT-133/conglobatin products.

Streptomyces pactum addition to contaminated mining soils improved soil quality and enhanced metals phytoextraction by wheat in a green remediation trial

Beneficial microbes can mitigate biotic or abiotic stress-induced damage to plant cell membranes. Yet, little is known about the effects of actinomycetes on cell membrane permeability in plants under joint biotic and abiotic stresses. Herein, the effects of three biocontrol actinomycetes (Streptomyces pactum Act12, S. globisporus Act7, and S. globisporus subsp. globisporus C28) on cell membrane permeability in the leaves of celery (Apium graveolens L. cv. “Hanyusiji”) were evaluated under fungal pathogen (biotic) and freezing (abiotic) stresses by using electrical conductivity measurements. Our results showed that, under freezing stress, any of three fungal pathogens alone resulted in increased cell membrane permeability. Under the single stress of freezing, medium and high concentrations of C28 respectively reduced cell membrane permeability by 37.0% and 30.6%; Act7 exerted no significant effects, whereas high concentration of Act12 increased cell membrane permeability. Under the dual stresses of fungal pathogen and freezing, these protective effects of Act12, Act7, and C28 did not differ significantly. Nonetheless, these protective effects depended on the type of pathogen infection involved: the largest reduction in cell membrane permeability occurred in the presence of F. oxysporum f. sp. vasinfectum (46.4-69.2%) followed by A. alternate (17.4-51.8%), with F. sambucinum ranked lowest (8.8-35.5%). In conclusion, inoculating an appropriate concentration of actinomycetes can mitigate freezing-induced cell membrane injury in celery plants. Importantly, the actinomycete strains better protected the cell membrane against freezing injury under fungal pathogen stress, but this benefit depends on the adverse effects of pathogens on cell membrane permeability.

Totopotensamide A (TPM A, 1) is a polyketide-peptide glycoside featuring a nonproteinogenic amino acid 4-chloro-6-methyl-5,7-dihydroxyphenylglycine (ClMeDPG). The biosynthetic gene cluster (BGC) of totopotensamides (tot) was previously activated by manipulating transcription regulators in marine-derived Streptomyces pactum SCSIO 02999. Herein, we report the heterologous expression of the tot BGC in Streptomyces lividans TK64, and the production improvement of TPM A via in-frame deletion of two negative regulators totR5 and totR3. The formation of ClMeDPG was proposed to require six enzymes, including four enzymes TotC1C2C3C4 for 3,5-dihydroxyphenylglycine (DPG) biosynthesis and two modifying enzymes TotH (halogenase) and TotM (methyltransferase). Heterologous expression of the four-gene cassette totC1C2C3C4 led to production of 3,5-dihydroxyphenylglyoxylate (DPGX). The aminotransferase TotC4 was biochemically characterized to convert DPGX to S-DPG. Inactivation of totH led to a mutant accumulated a deschloro derivative TPM H1, and the ΔtotHi/ΔtotMi double mutant afforded two deschloro-desmethyl products TPMs HM1 and HM2. A hydrolysis experiment demonstrated that the DPG moiety in TPM HM2 was S-DPG, consistent with that of the TotC4 enzymatic product. These results confirmed that TotH and TotM were responsible for ClMeDPG biosynthesis. Bioinformatics analysis indicated that both TotH and TotM might act on thiolation domain-tethered substrates. This study provided evidence for deciphering enzymes leading to ClMeDPG in TPM A, and unambiguously determined its absolute configuration as S.