Streams In Agricultural Areas Research Articles

Seasonally variable interactions between dissolved organic matter and mineral particles in an agricultural river

Streams and rivers form an important link in the global carbon cycle by transporting and transforming large amounts of carbon imported from terrestrial ecosystems to the oceans. Since streams in agricultural areas often experience increased concentrations of suspended mineral particles from soil erosion, they are important sites where dissolved organic carbon (DOC) may be adsorbed to particles and retained in the sediment. As the extent of adsorption varies with the molecular composition of dissolved organic matter (DOM), which is seasonally variable, we expect also the fraction of organic material that adsorbs to mineral particles to fluctuate over time. We sampled the agriculturally impacted River Fyrisån (Sweden) monthly during 1 year, and measured DOC concentration and DOM composition based on several optical properties. At each sampling occasion, we estimated the potential for adsorption by exposing the samples to a reference clay. The potential for adsorption was greatest when riverine DOM had the most terrestrial character, as this fraction of the DOM pool was selectively adsorbed to clay surfaces. The extent of adsorption was negatively related to the concentration of base cations, most notably calcium. We suggest that the observed relationships between the potential for adsorption, DOM composition and base cations are linked by discharge. A bioavailability test at one sampling occasion suggested that DOM remaining after exposure to clay particles was more biodegradable. This implies that adsorption may alter the degradation potential of DOM remaining in solution, which could have far reaching effects on the fate of organic carbon.

Investigation of the potential endocrine effect of nitrate in zebrafish Danio rerio and brown trout Salmo trutta

Nitrate has the potential to affect steroid production. Nitrate concentrations in streams in agricultural areas may exceed concentrations showing effects in laboratory studies. The effects of nitrate and/or nitrite on endocrine relevant endpoints were tested in zebrafish and brown trout. Zebrafish were exposed in two experiments to nitrate (8.8 to 89 mg NO3−/L) and nitrite (3.6 to 19 mg NO2−/L) during the period of sexual differentiation and sex ratios were determined. Vitellogenin concentrations were determined in the second experiment. The sex ratio was unaffected by the exposure to nitrate and nitrite. Vitellogenin concentrations were slightly elevated in males (but not females) in all of the groups exposed to nitrate. Juvenile brown trout were exposed to 5.7, 14, and 31 mg NO3−/L for 8 days and vitellogenin levels in liver were determined. Vitellogenin concentrations in the females were not affected by exposure, but in the males, there was an overall statistically significant effect of exposure to nitrate with the group exposed to 5.7 mg NO3−/L showing a trend of higher vitellogenin concentrations than the control group; levels in the males of the groups exposed to 14 and 31 mg NO3−/L were not statistically different from those of the control group. In conclusion, some marginal effect of nitrate in male fish on endocrine activity was observed but the present results for zebrafish, using environmentally relevant concentrations, do not define nitrate and nitrite as endocrine disrupting chemicals according to the generally accepted WHO/IPCS definition because no adverse effects (altered sex ratios) were demonstrated.

Legal ecotones: A comparative analysis of riparian policy protection in the Oregon Coast Range, USA

Waterways of the USA are protected under the public trust doctrine, placing responsibility on the state to safeguard public resources for the benefit of current and future generations. This responsibility has led to the development of management standards for lands adjacent to streams. In the state of Oregon, policy protection for riparian areas varies by ownership (e.g., federal, state, or private), land use (e.g., forest, agriculture, rural residential, or urban) and stream attributes, creating varying standards for riparian land-management practices along the stream corridor. Here, we compare state and federal riparian land-management standards in four major policies that apply to private and public lands in the Oregon Coast Range. We use a standard template to categorize elements of policy protection: (1) the regulatory approach, (2) policy goals, (3) stream attributes, and (4) management standards. All four policies have similar goals for achieving water-quality standards, but differ in their regulatory approach. Plans for agricultural lands rely on outcome-based standards to treat pollution, in contrast with the prescriptive policy approaches for federal, state, and private forest lands, which set specific standards with the intent of preventing pollution. Policies also differ regarding the stream attributes considered when specifying management standards. Across all policies, 25 categories of unique standards are identified. Buffer widths vary from 0 to ∼152 m, with no buffer requirements for streams in agricultural areas or small, non-fish-bearing, seasonal streams on private forest land; narrow buffer requirements for small, non-fish-bearing perennial streams on private forest land (3 m); and the widest buffer requirements for fish-bearing streams on federal land (two site-potential tree-heights, up to an estimated 152 m). Results provide insight into how ecosystem concerns are addressed by variable policy approaches in multi-ownership landscapes, an important consideration to recovery-planning efforts for threatened species.

Combination effects of pyrethroids and neonicotinoids on development and survival of Chironomus riparius

Standard ecotoxicological risk assessments are conducted on individual substances, however monitoring of streams in agricultural areas has shown that pesticides are rarely present alone. In fact, brief but intense pulse events such as storm water runoff and spray drift during application subject freshwater environments to complex mixtures of pesticides at high concentrations. This study investigates the potential risks to non-target aquatic organisms exposed to a brief but intense mixture of the neonicotinoid pesticides imidacloprid and thiacloprid and the pyrethroid pesticides deltamethrin and esfenvalerate, compared to single substance exposure. All four of these pesticides have been detected in surface waters at concentrations higher than benchmark values and both classes of pesticides are known to exert adverse effects on non-target aquatic organisms under single substance exposure scenarios. First instar midge larvae of the non-target aquatic organism, Chironomus riparius, were exposed to combinations of these four pesticides at 50% of their LC50 (96h) values in a 1h pulse. They were then reared to adulthood in uncontaminated conditions and assessed for survival, development time and fecundity. Our results show that the risk of disruption to survival and development of non-target aquatic organisms under this scenario is not negligible on account of the significant increases in mortality of C. riparius found in the majority of the pesticide exposures and the delays in development after pyrethroid exposure. While none of the deleterious effects appear to be amplified by combination of the pesticides, there is some evidence for antagonism. No effects on fecundity by any of the pesticide treatments were observed.

Estrogenic activity in drainage water: a field study on a Swiss cattle pasture

Dairy cow manure applied to pastures is a significant potential source of estrogenic contamination in nearby streams. One possible pathway is through infiltration via preferential flow to drainage pipes, particularly after heavy rainfall events. In a period of 73 days in the spring of 2010, a drainage catchment in a cattle pasture in the Swiss lowlands was closely monitored. Manure was applied three times during the study, and part of the catchment was also subjected to grazing. During five field campaigns, water samples from two sampling locations were taken for 4–24 h in consecutive sampling intervals. 17β-estradiol equivalents (EEQ) were determined with the yeast estrogen screen (YES) and the ER-CALUX assay. Some water chemistry parameters, pH, conductivity, oxygen content and soil moisture tension were also monitored. Washout of estrogenic activity was highest during or right after heavy rainfall events, shortly after manure spreading, when peak values of >10 ng/l EEQ were found in several samples. However, in two field campaigns, high EEQ values were also found 14 and 28 days, after the last manure application, in one case during a dry weather period. This indicates that estrogenic compounds are more stable in natural soils than what is expected from data gathered in lab studies. Streams in agricultural areas with a high proportion of drained land may be subject to numerous peaks of EEQ during the course of the year. This may have a negative effect on aquatic organisms, namely fish embryos, living in these streams.

Factors influencing nitrate within a low-gradient agricultural stream

In low-gradient agricultural streams, the proportion of land use devoted to agriculture, the sinuosity of the stream and the time of year influence the concentration of nitrate in the stream waters. Land use influences the source of nitrate and also the morphology of the stream. Greater agricultural land use weakly correlated (r = 0.67) to higher nitrate concentrations. Streams in agricultural areas have been straightened, which decreases the sinuosity. As a stream meanders and becomes more sinuous, the potential for lateral hyporheic flow increases, which can enhance a stream system’s ability to remove nitrate. Logically, higher sinuous streams should remove more nitrate and likely sulfate as there is a greater potential for lateral hyporheic flows. To test this hypothesis, nitrate and sulfate were monitored. Mass fluxes of nitrate along six stream segments with varying sinuosity values were calculated and statistically analyzed to assess if differences in mass fluxes along the segments existed. Along the segments, there are statistically significant differences in the mass fluxes of nitrate [F(5,174) = 4.777; p = 0.001]. Stream segments with higher sinuosity index values exhibited a loss or lower gain in nitrate and sulfate than lower sinuosity index segments. The data suggest that stream segments with high sinuosity indices provide greater stream distance and increased hyporheic interaction within the streambed. Additionally, the more sinuous segments provide for an increase in lateral hyporheic flow beneath meander lobes. These additional hyporheic flows lead to enhanced denitrification in low-gradient agricultural streams. Seasonal differences were also noted. August through October experienced the lower nitrate concentrations as compared to June and July which exhibited the highest nitrate concentrations.

Active <i>in Situ</i> Biomonitoring of Pesticide Pulses Using <i>Gammarus</i> spp. in Small Tributaries of Lake Constance

Gammarids are important members of a stream’s macrozoobenthos biocoenosis and food web. Moreover, they proved to be very sensitive towards different types of pollution. GamToxTM is a new in situ ecotoxicity test, based on survival and feeding behavior of caged gammarids for active monitoring of small streams in agricultural areas. GamToxTM has been applied in two streams with specific pollution problems in the catchment of Lake Constance. Ten organisms were exposed in 5 replicates in flow through test tubes containing one conditioned alder leaf, placed in baskets which were attached in the stream bottom and on the banks. Each week, the number of living animals was counted, the percentage of leaf skeletized estimated in semi-quantitative classes and a new elder leaf provided. Dead organisms were removed. Simultaneously, chemical analyses of pesticides and nutrients (N-compounds, P) were performed on cumulative water samples over one week. Moreover, macrozoobenthos was collected and determined according to the IBCH method, and the SPEAR index calculated. GamToxTM proved to be very sensitive to detect pesticides, copper as well as nutrients, both during acute pollution pulses and chronic exposures of up to 6 weeks. Survival turned out to be a more sensitive and less variable parameter than feeding. GamToxTM is easy to perform and directly provides a measure of ecotoxicological effects of toxicant/nutrient mixtures, which cannot be predicted by biological indices based on macrozoobenthos data such as IBCH and SPEAR-index. This study was co-financed by the InterReg IV project “?kotoxikologischer Index im Bodenseeraum”, no. 227 (2011-2013) supported by the EFRE.

Atrazine reduces reproduction in fathead minnow ( Pimephales promelas)

Atrazine, the widely used herbicide, has shown to affect the hypothalamus–pituitary–gonad axis in certain vertebrate species, but few studies have examined reproductive effects of this chemical on fish. Our study was designed to evaluate a population endpoint (egg production) in conjunction with histological (e.g., gonad development) and biochemical (e.g., hormone production) phenotypes associated with atrazine exposure in fathead minnows. Adult virgin breeding groups of 1 male and 2 females were exposed to nominal concentrations of 0, 0.5, 5.0, and 50 μg/L of atrazine in a flow-through diluter for 14 or 30 days. Total egg production was lower (19–39%) in all atrazine-exposed groups as compared to the controls. The decreases in cumulative egg production of atrazine treated fish were significant by 17–20 days of exposure. Reductions in egg production in atrazine treatment groups were most attributable to reduced numbers of spawning events with increased atrazine exposure concentrations. Gonad abnormalities were observed in both male and female fish of atrazine-exposed fish. Our results also indicate that atrazine reduces egg production through alteration of final maturation of oocytes. The reproductive effects observed in this study warrant further investigation and evaluation of the potential risks posed by atrazine, particularly feral populations of fish from streams in agricultural areas with high use of this herbicide.

Microbial pollution of water from agriculture

Microbial contamination of small streams in agricultural areas was monitored for two years. Microbiological indicators of faecal pollution (faecal coliforms, <I>Escherichia coli</I> and intestinal enterococci were detected by standard methods based on the cultivation of bacteria on selective media). The obtained results showed that running contamination of streams from agricultural areas was not extremely high, but it showed marked seasonal fluctuations (the average values and maximal values revealed great differences). Microbial contamination also increased several times in relation to high precipitation. The water quality in three (and/or four) localities exceeded the acceptable counts of faecal coliforms and enterococci given by the Czech legislation (40 CFU/ml for faecal coliforms and 20 CFU/ ml for enterococci). In agriculturally polluted streams, there were detected more enterococci than faecal coliforms, and also some less frequent species related to farm animals (<I>Streptococcus equines</I> and <I>S. bovis</I>) or plant rests (<I>E. mundtii, E. gallinarum, E. casseliflavus</I>) were present. <I>E. faecalis</I> and <I>E. faecium</I> strains (these are the most common species related to human faecal pollution) were less frequent there.

Agricultural land use and black fly (Diptera, Simuliidae) species richness and species assemblages in tropical streams, Northeastern Thailand

Habitat degradation through agricultural land use is the major factor threatening lotic ecosystems. Although black flies are major components of these ecosystems, the impact of agricultural land use on species diversity and species assemblages has been largely ignored in tropical streams of the Oriental region. The objectives of this study are to examine patterns of species distribution and species richness and to compare black fly species richness and species assemblages in forest and agricultural streams in Thailand. A total of 143 collections were made from 70 stream sites between June 2007 and May 2008. Whereas 19 black fly species found in these collections were all found in forest sites, only 13 species were found in agricultural sites. High species richness was associated with larger, faster, and cooler streams with larger streambed particles and the presence of riparian trees. Logistic regression analyses revealed that stream size, velocity, and riparian vegetation are among the most important factors determining patterns of spatial distribution. The results are largely consistent with studies in other zoogeographic regions, suggesting the existence of general rules for black fly species distributions. Comparisons of the physicochemical conditions between forest and agricultural streams indicated that streams in agricultural areas are warmer, with higher conductivity and fewer riparian trees. Species richness was significantly higher in forest than in agricultural streams (t = 3.61, P 20%) of the sampling sites. In contrast, streams in agricultural areas were predominantly occupied by S. aureohirtum (>80%) among the sole black fly species at 27% of the sites. The results indicate that agricultural land use has a significantly detrimental impact on black fly diversity and species assemblages.

A comparison of predicted and measured levels of runoff-related pesticide concentrations in small lowland streams on a landscape level

Short-term pollution events via runoff are typical of streams in agricultural areas. Existing runoff models that simulate pesticide loss from agricultural fields require extensive input of information. There is thus a need for a simple model that can predict runoff-related pesticide concentrations in many streams on a landscape level when only limited data are available. To validate such a model, the runoff-related pesticide load of 18 small lowland streams was predicted with an extended version of the model “ simplified formula for indirect loadings caused by runoff” (available from the Organisation for Economic Cooperation and Development, OECD). The authors suggest that the model presented here is suitable for use in routine exposure assessment of pesticides on a landscape level, as all input data (soil, slope, precipitation, pesticide application) are readily available from public authorities or could be generated by simple regional flood hydrograph curves. The predicted concentrations were compared with measured concentrations obtained by runoff-triggered sampling. Fungicides, insecticides and herbicides were detected in 17 streams, with max. concentrations measuring up to 29.7 μg/l for the fungicide azoxystrobin and 0.3 μg/l for the insecticide parathion-ethyl. Herbicides were detected in 16 streams, with max. concentrations between 13.7 and 1.2 μg/l. The linear regression between the predicted and measured concentrations (log-values) shows significant correlations for the following pesticides: azoxystrobin: r 2=0.43; p=0.03; epoxiconazole: r 2=0.71; p⩽0.01; tebuconazole: r 2=0.77; p⩽0.01. The present model successfully explains the pesticide concentrations associated with single entry events caused by runoff, especially at concentration levels above (⩾0.5 μg/l).

Transport and fate of nitrate in headwater agricultural streams in Illinois.

Nitrogen inputs to the Gulf of Mexico have increased during recent decades and agricultural regions in the upper Midwest, such as those in Illinois, are a major source of N to the Mississippi River. How strongly denitrification affects the transport of nitrate (NO(3)-N) in Illinois streams has not been directly assessed. We used the nutrient spiraling model to assess the role of in-stream denitrification in affecting the concentration and downstream transport of NO(3)-N in five headwater streams in agricultural areas of east-central Illinois. Denitrification in stream sediments was measured approximately monthly from April 2001 through January 2002. Denitrification rates tended to be high (up to 15 mg N m(-2) h(-1)), but the concentration of NO(3)-N in the streams was also high (>7 mg N L(-1)). Uptake velocities for NO(3)-N (uptake rate/concentration) were lower than reported for undisturbed streams, indicating that denitrification was not an efficient N sink relative to the concentration of NO(3)-N in the water column. Denitrification uptake lengths (the average distance NO(3)-N travels before being denitrified) were long and indicated that denitrification in the streambed did not affect the transport of NO(3)-N. Loss rates for NO(3)-N in the streams were <5% d(-1) except during periods of low discharge and low NO(3)-N concentration, which occurred only in late summer and early autumn. Annually, most NO(3)-N in these headwater sites appeared to be exported to downstream water bodies rather than denitrified, suggesting previous estimates of N losses through in-stream denitrification may have been overestimated.

Pesticides in stream water within an agricultural catchment in southern Sweden, 1990–1996

Pesticide loss to stream water was studied in a small agricultural catchment in southern Sweden during the period 1990–1996. A total of 38 pesticides were detected in water samples, including 30 herbicides, four fungicides, three insecticides and one metabolite of one of the herbicides. Concentrations of pesticides in stream water were observed throughout the sampling periods. Peak concentrations occurred during the spraying seasons and following runoff events. Daily average concentrations sometimes varied by one order of magnitude from one day to another. Pesticides were also found in water samples as a result of incautious actions during handling and application procedures. Concentrations were lower at the outlet of the catchment area when the water had passed an open part of the stream, compared to concentrations detected in discharge from a culvert system upstream. This was largely a result of dilution from groundwater intrusion during low-flow periods. Sampling at different sites along the culvert demonstrated that the small village situated in the catchment did not contribute to pesticide findings in the culvert discharge. Wind drift had little influence on stream-water quality. Pesticide application for weed control in farmyards resulted in a substantial contribution to the pesticide load in stream water. Pesticides were persistent in the discharge throughout the winter and originated from both autumn and spring applications, as well as from farmyard application. Some autumn applied pesticides prevailed in stream flow during the following summer. Total amounts of pesticides lost in stream flow during May–September each year varied between 0.5 and 2.8 kg during the 7-year period, corresponding to ∼0.1% of the applied amount. Losses of single pesticides were generally less than 0.3% of the applied amount during individual years. Pesticides from agricultural applications in the catchment constituted, on average, 82% of the total transported amount lost during May–September each year, of which 2% was from autumn application the previous year. There was an overall correlation between amounts used in the catchment and occurrence in the water samples. The total pesticide load in water decreased markedly during the course of the investigation, in accordance with decreased amounts applied during spring and early summer. The results indicate that concentrations of some pesticides entering head-water streams in agricultural areas are close to, and during certain time periods even above those levels demonstrated as having an impact on the aquatic flora and fauna.

Influence of substrate type and physico‐chemical conditions on macroinvertebrate faunas and biotic indices of some lowland Waikato, New Zealand, streams

Lowland streams in agricultural areas are often considered degraded because of high turbidity, high biomass of adventive macrophytes, and a predominance of fine bed substrates. Invertebrate faunas in these streams are being increasingly used in biomonitoring, and an understanding of factors affecting their distribution and abundance is important for interpreting the results of these studies. We measured physico‐chemical parameters and sampled macroinvertebrate faunas on inorganic substrates, wood, and macrophytes to investigate factors influencing invertebrate distribution and abundance in Waikato, New Zealand, lowland streams, and to evaluate six biotic indices and a rapid bioassessment protocol for lowland stream environments. At most sites, invertebrate faunas were dominated numerically on all substrate types by the hydrobiid snail Potamopyrgus antipodarum (Gray), the eusirid amphipod Paracalliope, and Diptera (mostly the chironomid Neonella forsythii Boothroyd and the blackfly Austrosimulium spp.). Stepwise regression analyses indicated that densities of some common taxa on inorganic substrates (0.1 m‐2) and macrophytes (g‐1 wet weight) were correlated with combinations of reach water depth, percentage of bed covered by macrophytes or sand/silt, and water quality factors including conductivity, water temperature, and measures of dissolved oxygen (DO) concentration and magnitude of diel fluctuation. These factors and also the amount of wood present explained 36–87% of the variation in several of the biotic indices examined on different substrate types. Some taxonomic (no. of taxa) and compositional (% dominant taxon) indices appeared sensitive to measured habitat conditions, and this may affect their utility for detecting water quality impacts in lowland streams. Percent Ephemerop‐tera, Plecoptera, and Tricoptera (EPT), and the Macroinvertebrate Community Index (MCI) and its quantitative analogue (QMCI) reflected factors thought to be more associated with direct and indirect effects of riparian and catchment land use on water quality (DO, conductivity, temperature, macrophyte cover). Comparison of indices derived from intensive and rapid bioassessment sampling yielded significant regressions with slopes that were not significantly different to 1 for % EPT and MCI. These indices appear to be particularly useful for rapid bioassessment of lowland stream environments.

Life history and secondary production of Cheumatopsyche spp. in a small Appalachian stream with two different land uses on its watershed

We studied life history and secondary production of Cheumatopsyche spp. (Trichoptera: Hydropsychidae) in StroublesCreek, a 3rd order stream near Blacksburg, Virginia to compare lifehistories and production dynamics in two reaches with differentland-uses. The upstream reach was influenced by agriculturalactivities and more annual degree-days accumulated in this sectionof the stream. The downstream reach was surrounded by forest,received less thermal input than the upstream reach, and showedlittle evidence of human disturbance. Differences between the twosites included a shift in dominant taxa and changes in voltinismand production of hydropsychids. Cheumatopsyche pettiti(Banks) was dominant at the upstream site, while C. pettitiand Cheumatopsyche oxa Ross were both abundant downstream.Cheumatopsyche larvae and other aquatic insects had higherdensities at the forested downstream site. C. pettiti wasbivoltine at the upstream site, but C. pettiti and C. oxa were partially bivoltine downstream, with a small summergeneration overlapping with continuous emergence of overwinteringlarvae. Cheumatopsyche production at the two sites inStroubles Creek was higher than in forested, low-order streams ofNorth America, but similar to some values obtained at streams inagricultural areas and large rivers. Production was higher at thedownstream site, but the superior bivoltinism at the upstream sitecounterbalanced, to some extent, the increase in larval densitythat caused higher production downstream. The adjustment in lifehistory and production dynamics, in the two reaches, showed theecological plasticity of Cheumatopsyche and the importance ofhydropsychids in the energy flow of moderately disturbedstreams.

Isometric Plant Viruses in Ditches and Streams in Agricultural Areas: Recovery of Previously Found Viruses and Identification of hitherto Unrecorded Carmoand Tombusviruses Including Grapevine Algerian Latent Virus

AbstractDuring a survey of plant viruses in ditches and streams in agricultural areas we obtained sixteen isolates of isometric viruses. Seven of them were identified as carnation ringspot dianthovirus and four as tobacco necrosis necrovirus which both had already been detected in water samples in previous occasions. Three of the remaining isolates proved to be tombusviruses and two to be carmoviruses. One of the tombusviruses was found to be closely related to grapevine Algerian latent virus. For the other tombusvirus (two isolates) and the two carmoviruses the natural hosts are unknown and serological relationships with other viruses of the same group were not found. The assignment to the respective groups was based on particle morphology, coat protein molecular weight, cDNA hybridization tests and cytopathological effects.Zusammenfassung Isometrische Pflanzenviren in Gräben und Bächen in landwirtschaftlich genutzten Gebieten: Erneuter Nachweis bereits früher gefundener Viren und Identifizierung bisher nicht nachgewiesener Carmo‐ und Tombusviren einschließlich grapevine Algerian latent virus Bei Untersuchungen über das Vorkommen von pflanzenpathogenen Viren in Gräben und Bächen in landwirtschaftlich genutzten Gebieten erhielten wird sechzehn Isolate von isometrischen Viren. Sieben davon erwiesen sich als carnation ringspot virus, vier als tobacco necrosis virus; beide Viren waren auch früher schon in Oberflächengewässern nachgewiesen worden. Drei der übrigen Isolate konnten als Tombus‐ und zwei weitere als Carmoviren identifiziert werden. Das eine Tombusvirus zeigte eine enge serologische Verwandtschaft mit dem grapevine Algerian latent virus. Für das andere Tombusvirus (zwei Isolate) und die beiden Carmoviren sind natürliche Wirte nicht bekannt. Serologisch zeigten sie keine Verwandtschaft zu den bekannten Vertretern der entsprechenden Gruppen. Die Gruppenzuordnung erfolgte auf Grund der Partikelmorphologie, des Molekulargewichtes des Hüllproteins, der Ergebnisse von cDNA‐Hybridisicrungstesten und der zytopathologischen Effekte.