A collective Thomson scattering (CTS) diagnostic system based on a pulsed CO2 laser has been developed and brought into operation to establish a measurement technique for ion temperature and the energy spectrum of fast ions. The pulsed CO2 laser (wavelength 10.6 μm, beam energy 15 J, pulse width 1 μs) and a heterodyne receiver were installed on the JT-60U tokamak. Calculation of the scattered power spectrum from high temperature plasma in JT-60U shows that a good signal-to-noise value is expected for the bulk-ion temperature measurement. Calibration of the heterodyne receiver system has been carried out using a large area (12×12 cm2) blackbody radiation source. Commissioning of the CTS system by injecting the CO2 laser into the plasmas has been accomplished. However, a scattered signal has not yet been detected due to electrical noise originating from the pulsed lasers discharge and stray signal coming from mode impurities in the pulsed laser. After the electrical and stray light reduction, ion temperature will be evaluated from the scattered spectrum using the CTS system.