Research Strategy Research Articles

Exploring amyotrophic lateral sclerosis through the visual system: A systematic review

AbstractBackground and PurposeThe human visual system relies on neural networks throughout the brain that are easily accessible for tests exploring eye structures and movements. Over the past two decades, investigations have been carried out on both afferent and efferent components of the visual system in people with amyotrophic lateral sclerosis (ALS). This approach might represent an innovative biomarker research strategy to better characterise the phenotypic variability of ALS. The purpose of this review was to determine whether exploring the visual system of patients with ALS (pwALS) is an effective strategy.MethodsThe Medline and Web of science databases were searched for studies with terms relating to ALS and vision. Of 1146 references identified, 43 articles were included.ResultsIn this review article, both afferent and efferent components of the visual system were found to be impaired in pwALS in the absence of visual complaint, thereby contributing to the hypothesis that ALS is a multisystem disease with sensory involvement. Of note, some areas of the eye remain unexplored (i.e., tears, and retinal function using electroretinography).ConclusionsAccording to the findings available in the literature, investigating the oculomotor system and exploring the ocular surface could represent two key promising strategies to identify new diagnostic biomarkers in pwALS. Further longitudinal studies are needed to identify relevant indicators of disease progression and response to therapeutic intervention.

Lactylation: A Novel Post-Translational Modification with Clinical Implications in CNS Diseases

Lactate, an important metabolic product, provides energy to neural cells during energy depletion or high demand and acts as a signaling molecule in the central nervous system. Recent studies revealed that lactate-mediated protein lactylation regulates gene transcription and influences cell fate, metabolic processes, inflammation, and immune responses. This review comprehensively examines the regulatory roles and mechanisms of lactylation in neurodevelopment, neuropsychiatric disorders, brain tumors, and cerebrovascular diseases. This analysis indicates that lactylation has multifaceted effects on central nervous system function and pathology, particularly in hypoxia-induced brain damage. Highlighting its potential as a novel therapeutic target, lactylation may play a significant role in treating neurological diseases. By summarizing current findings, this review aims to provide insights and guide future research and clinical strategies for central nervous system disorders.

Multiscale Analysis of Pedestrian Crossing Distance

Problem, research strategy, and findings Crossing distance is considered a factor in both pedestrian safety and behavior. However, it has seldom been quantified within or across entire cities or related to walking outcomes. Combining data encoded in OpenStreetMap and drawn from high-resolution satellite imagery, we measured formal pedestrian crossings throughout a dense European city (Paris [France]), a dense American city (San Francisco [CA]), and a less-dense, more car-centric American city (Irvine [CA]). This granular approach—covering roughly 49,000 total crossings—identified inter- and intraurban spatial patterns in the distribution of pedestrian crossing distance, including clusters of long crossings that likely deter walking and increase its risk. By overlaying recent pedestrian–vehicle collisions on these novel data sets we found that longer crossing distance correlated with increased likelihood of collisions, raising the salience of traffic-calming interventions. Takeaway for practice Quantifying pedestrian crossing distance at the scale of entire municipalities empowers transportation planners to identify pedestrian-hostile crossings (individuals and clusters), add context to collision trends, and geographically target locations for traffic calming. These cases collectively demonstrate how the increasing prioritization of the automobile in city planning quantitatively changes (and degrades) the pedestrian environment, as well as how low-tech investments, such as sidewalk extensions and refuge islands, can mitigate these trends.

What makes activities strategic: Toward a new framework for strategy‐as‐practice research

AbstractResearch AbstractStrategy as practice is one of the most vibrant approaches to strategy research. Yet, there is significant ambiguity around what characterizes an activity as strategic and thus as falling into the domain of strategy as practice. In this article, we address this fundamental concern by differentiating four distinctive views of what qualifies activities as strategic: (1) activities that have important consequences, (2) activities that are labeled strategic, (3) activities carried out by strategists, and (4) activities that perform an important recurrent pattern. Each of these views is associated with different research questions resulting in different research insights. We discuss how the four views together form a new research framework that expands the notion of strategy and thereby the research domain of strategic management.Managerial SummaryStrategy as practice is an important approach to studying strategic management that focuses on strategic activities. However, there is significant ambiguity around what characterizes activities as strategic. In this article, we identify four different views on this question: (1) activities that have important consequences, (2) activities that are labeled strategic, (3) activities carried out by strategists, and (4) activities that perform an important recurrent pattern of activities. Each of these views is associated with different questions and thus with different types of insights. We suggest that the four views together form a research framework that reveals distinctive links between strategy as practice and other lines of strategy research and that expands our notion of strategy and thereby the domain of strategic management.

Mechanism of norcantharidin intervention in gastric cancer: analysis based on antitumor proprietary Chinese medicine database, network pharmacology, and transcriptomics

BackgroundCombining antitumor proprietary Chinese medicine (pCm) with radiotherapy and chemotherapy can effectively improve tumor cure rates and enhance patients’ quality of life. Gastric cancer (GC) severely endangers public health. Despite satisfactory therapeutic effects achieved by using antitumor pCm to treat GC, its underlying mechanism remains unclear.ObjectiveTo integrate existing research data, construct a database of antitumor pCm, and study the intervention mechanisms in GC by focusing on their monomer components.MethodsWe constructed an antitumor pCm database based on China’s medical insurance catalog, and employed network pharmacology, molecular docking methods, cell experiments, transcriptomics, and bioinformatics to investigate the intervention mechanisms of effective pCm components for GC.ResultsThe study built an antitumor pCm database including 55 pCms, 171 Chinese herbal medicines, 1955 chemical components, 2104 targets, and 32 disease information. Network pharmacology and molecular docking technology identified norcantharidin as an effective component of antitumor pCm. In vitro experiments showed that norcantharidin effectively inhibited GC cell proliferation, migration, and invasion; blocked the G2/M cell cycle phase; and induced GC cell apoptosis. Transcriptomic results revealed that norcantharidin affected biological processes, such as cell adhesion, migration, and inflammatory responses by influencing PI3K-AKT, NF-κB, JAK-STAT, TNF-α signaling pathways, and EMT-related pathways. Core molecules of norcantharidin involved in GC intervention include SERPINE1, SHOX2, SOX4, PRDM1, TGFR3, TOX, PAX9, IL2RB, LAG3, and IL15RA. Additionally, the key target SERPINE1 was identified using bioinformatics methods.ConclusionNorcantharidin, as an effective component of anti-tumor pCm, exerts its therapeutic effects on GC by influencing biological processes such as cell adhesion, migration, and inflammation. This study provides a foundation and research strategy for the post-marketing re-evaluation of antitumor pCms.

Numerical simulation of heat transfer properties of skin tissue acted on repetitive laser irradiation

Pulsed laser has been widely used in the clinical treatment of various diseases and injuries due to its unique advantages in the process of interaction with skin tissue. In order to ensure the therapeutic effect and patient safety, it is necessary to accurately monitor the time-space response of the whole-field temperature of the target tissue irradiated by pulsed laser. A research strategy to explore the thermal response and incidental thermal damage of skin tissue induced by repeated pulses laser has been proposed in present work. A theoretical model involved variable physical properties was first proposed, in which the dual-phase lag (DPL) equation and Henrique burn equation were employed to evaluate the tissue temperature and thermal damage induced by repeated pulse laser irradiation. With the help of finite difference method, the governing equation with variable physical properties was then numerical solved to obtain the time-space distribution of tissue temperature, and the evolution process of the thermal damage was further calculated. The effects of laser input parameters and variable physical parameters on the laser penetration depth, temperature distribution and burn degree of the irradiated tissue were analyzed.

Implementation of Games as a Pedagogical Strategy to Enhance Meaningful Learning of Mathematics

Objective: The objective of this study is to investigate how the implementation of a pedagogical strategy supported by playfulness affects the improvement of meaningful learning in mathematics. Theoretical Framework: The game as a didactic resource has been used in the teaching of mathematics with different purposes in this study its use is supported by the Colombian legislation on education and recreation of youth. Method: The methodology adopted for this research comprises an action research strategy using an experimental design with a sample of 35 students and a control group of the same number. Data collection was carried out using a Likert scale. Results and Discussion: The results obtained revealed that better results were obtained in the written test after the pedagogical intervention. In addition, it was observed that the attitude towards mathematics was reinforced. Research Implications: Based on the results, the opportunity arises to create guides and teaching materials based on the strategy of playful games used in the project. Originality/Value: This study offers empirical evidence on the usefulness of games as a didactic strategy not only to improve the learning of certain mathematical concepts but also to improve classroom coexistence.

How do Patients Receiving Antiretroviral Therapy (ART) at A Dedicated ART Center in Bhagalpur Demonstrate Observable Patterns and Outcomes in an Observational Study?

Ours study provides a comprehensive overview of the HIV/AIDS landscape in Bhagalpur, Bihar, focusing on patients receiving Antiretroviral Therapy (ART). Conducted over six years, involving 10,355 participants, the research explores demographic details, treatment outcomes, and treatment-seeking behaviors. Notably, 100% participation reflects the community's engagement, with detailed analyses of gender-specific registrations, treatment initiation, and outcomes. The study delves into patients' geographic origins, travel distances, and factors influencing their preferences. Insightful discussions on perceptions regarding HIV and ART underscore societal challenges. Positive aspects include family support and awareness, yet concerns like substance use and travel barriers need attention. The study concludes with recommendations for targeted interventions to enhance HIV/AIDS care effectiveness. Limitations highlight the need for broader community comparisons, emphasizing the study's contribution to shaping future research and healthcare strategies. The acknowledgment expresses gratitude for mentorship and collaborative efforts in completing the project.

Teacher Training: (Dis)encounter between Theory and Practice

The preparation of teachers is a key process in the development of effective teachers, however, initial training always seems insufficient and outdated to produce the expected teaching profile and respond to the dynamic character of today's society. This article reports part of the research "teachers in the process of initial training" (2020-2021), developed in the context of a Faculty of Education Sciences, Chile. Based on a qualitative methodology and a case study research strategy, we worked with 120 pedagogy students in the process of training, who offered their representations regarding the theory-practice relationship in the context of the Chilean educational system. In a complementary way, 5 focus groups were administered with the intention of enriching ideas and perspectives. The criteria to choose them were to be studying a pedagogy program, with experience of practices in schools and that they accepted to participate voluntarily, after signing consent. The results show that the theory-practice relationship can be understood in various ways: dependence, disagreement and integration which influence the way teaching is understood and its exercise. In terms of projections, the need arises to think of a training that in a bidirectional way manages to theorize the practice and experience the theory.

Retail on the Ground and on the Books: Vacancies and the (Mis)Match Between Retail Activity and Regulated Land Uses

Problem, research strategy, and findings Though demand for living near urban amenities has grown over the past two decades, retail vacancies continue to plague many urban areas. Are cities overzoning for retail? Can this overzoning explain the proliferation of urban retail vacancies in the past two decades? We first leveraged longitudinal data on retail leases to depict patterns of retail market activity in a sample of seven moderate to large U.S. cities. Second, we used property-level data on land use regulation to investigate whether institutional context, specifically zoning, has played a role in patterns of urban retail vacancy in the past two decades. We drilled down in New York City (NY) and Los Angeles (CA) to compare trends in retail leases with changes in regulated retail land use between 2006 and 2022. We show that in this period retail rents remained flat or declined and that the average retail space did not grow and may have shrunk. Our New York and Los Angeles microdata document that, during roughly this same period, the amount of land zoned for retail increased and that the building square footage zoned for retail grew even more rapidly. Indeed, during the same decades when retail leasing slowed and rents declined, the total amount of land and square footage regulated for retail use increased. Thus, our research indicates that, in addition to market forces, land use decisions have driven the current oversupply of retail space. This also suggests an important role for planning in solving the problem of vacancies. Takeaway for practice If macroeconomic factors drive the obsolescence of certain commercial uses, local zoning and comprehensive planning should respond accordingly. As cities emerge from the pandemic and adjust to new work–live patterns, planning for the volume and spatial distribution of retail will remain an important task for urban planners and managers.

Venous thrombosis and obesity: from clinical needs to therapeutic challenges.

Weight bias and stigma have limited the awareness of the systemic consequences related to obesity. As the narrative evolves, obesity is emerging as a driver and enhancer of many pathological conditions. Among these, the risk of venous thromboembolism (VTE) is a critical concern linked to obesity, ranking as the third most common cardiovascular condition. Obesity is recognized as a multifactorial risk factor for VTE, influenced by genetic, demographic, behavioral, and socio-economic conditions. Despite established links, the exact incidence of obesity related VTE in the general population remains largely unknown. The complexity of distinguishing between provoked and unprovoked VTE, coupled with gaps in obesity definition and assessment still complicates a tailored risk assessment of VTE risk. Obesity reactivity, hypercoagulability, and endothelial dysfunction are driven by the so-called 'adiposopathy'. This state of chronic inflammation and metabolic disturbance amplifies thrombin generation and alters endothelial function, promoting a pro-thrombotic environment. Additionally, the inflammation-induced clot formation-also referred to as 'immunothrombosis' further exacerbates VTE risk in people living with obesity. Furthermore, current evidence highlights significant gaps in the management of obesity related VTE, particularly concerning prophylaxis and treatment efficacy of anticoagulants in people living with obesity. This review underscores the need for tailored therapeutic approaches and well-designed clinical trials to address the unique challenges posed by obesity in VTE prevention and management. Advanced research and innovative strategies are imperative to improve outcomes and reduce the burden of VTE in people living with obesity.

Relationship between microstructure and dielectric/thermal management properties of biomass ramie fiber and its applications in recyclable wave-transparent composites

Industrial fiber crops are increasingly utilized in functional composites as substitutes for conventional fibers, aiming to address the limited microwave transmittance (MWT) and thermal conductivity (λ) of commercial glass fiber-reinforced composites (D-GFRC), which significantly reduce the service life of communication equipment shells and result in substantial waste pollution. Ramie fiber-reinforced composite (RFRC) possesses good dielectric/thermal management properties attributed to distinctive microstructure. However, the uncontrollable microstructure and unclear structure-property relationship of ramie fiber hinder its application in communication composites. Herein, the microstructures of ramie fiber, including aggregation and micro/nanopores structures, were successfully manipulated by coupling physical fields (temperature and force fields) with the guidance of molecular simulation. Subsequently, a clear relationship between microstructure and dielectric/thermal management properties was established. Moreover, leveraging this relationship, the performance of RFRC was optimized through coupled physical fields. Specifically, the MWT of RFRC reaches an impressive 95.9 %, and the λ is enhanced by 140 % compared to that of D-GFRC. Importantly, RFRC waste can be completely transformed into functional particles, thereby achieving a closed-loop utilization. This research strategy offers valuable insights into the regulation of microstructures and the development of recyclable wave-transparent composites derived from industrial crops.

Exploring sexual life enrichment: a journey into strengthening well-being for women post- menopause through qualitative study

Backgroundwomen post-menopause, are faced with various physical, emotional, and relational challenges. One such aspect that tends to be overlooked is the impact of menopause on sexual well-being. This study aimed to elucidate the concept of enriching the sexual life of women post-menopause.MethodsA qualitative research strategy was adopted using a conventional content analysis approach. Data collection was conducted through semi-structured interviews with 24 participants (17 women post-menopause and 7 experts), using purposive sampling.ResultsThe data analysis resulted in the extraction of 341 codes, 24 subcategories, and 8 categories. Ultimately, the following three themes emerged: “maintaining and enhancing the position of sexual relationships,” “deepening sexual relationships and expanding intimacy,” and “improving communication skills with the spouse “.ConclusionEnriching the sexual life of women post-menopause, as suggested by the themes, involves nurturing their relationships, keeping these connections strong and valued, deepening intimacy, and promoting effective communication to ensure a fulfilling and enjoyable experience during this phase of life. This leads to a sense of security, health, and tranquility, ultimately manifesting positive repercussions on the couple’s and family’s health.

The Implementation Strategy of Research and Innovation to Encourage the Self-Reliance of Pesantren Economic Institutions

Pesantren memiliki peran yang sangat penting dalam masyarakat Indonesia, baik dalam aspek pendidikan, agama, maupun ekonomi. Namun demikian, Pesantren mayoritas masih bergantung kepada donatur dan belum bisa mandiri secara ekonomi. Untuk mewujudkan kemandiriannya, memasifkan penetrasi penerapan riset dan inovasi ke pesantren menjadi hal yang wajib. Kajian ini meneliti aspek regulasi yang mendukung serta aspek riset dan inovasi yang tepat yang dapat diterapkan di pesantren sebagai bahan pertimbangan pemangku kebijakan menentukan arah pembangunan ekonomi pesantren. Rekomendasi hasil kajian ini adalah : (1) Kementerian Agama didorong untuk mengeluarkan regulasi terkait Kolaborasi Riset dan Inovasi pada Pesantren dengan BRIN dan Perguruan Tinggi. SDM IPTEK dari BRIN dan Perguruan Tinggi diarahkan menjadi pencetak “penyuluh iptek-risnov” yang membina pesantren di daerahnya. Penyuluh berorientasi untuk menumbuhkan semangat inovasi melalui intervensi Teknologi Tepat Guna dan Hasil Riset Inovatif dari lembaga riset baik dari BRIN maupun lembaga riset lainnya. (2) Pemerintah Daerah melalui BRIDA dan Bappeda menumbuhkan pesantren di daerahnya sebagai Innovative Economic Hub dengan menjamin keberlanjutan programnya melalui regulasi daerah. BRIDA menjadi Innovation Learning Hub dengan jejaringnya yang luas, Bappeda memastikan keberlanjutan yaitu dengan mengawal program pembangunan kemandirian pesantren dalam perencanaan pembangunan daerah.

On the Role of Group Competition Mechanism in International Chinese Classroom

International Chinese language education is a field that has attracted much attention in recent years. Under the background of China's increasing international influence, more and more people realize the importance of learning Chinese. However, with the development of international Chinese education, we should also pay attention to the research of international Chinese education classroom strategy. Based on the observation of online open courses, with group competition mechanism as the theoretical basis, take the class “Whose bag is this” in the first grade of a primary school in the Philippines as an example, observed the classroom group competition mechanism, this paper mainly introduces the competition mechanism into the Chinese language teaching classroom advantages and disadvantages, and further put forward the principles and methods of classroom competition mechanism. The aim is to provide new teaching ideas for Chinese teachers and facilitate and improve their teaching efficiency and quality.

Novel insights into immunopathogenesis and crucial biomarkers between primary open‐angle glaucoma and systemic lupus erythematosus

AbstractGlaucoma is the primary factor underlying irreversible blindness. Recent studies suggest that the risk of glaucoma significantly increases in patients with systemic lupus erythematosus (SLE); however, the mechanism underlying this association remains unclear. Therefore, this study aimed to identify novel common biomarkers and potential therapeutic drugs for SLE and glaucoma. GSE27276, GSE50772, and GSE148371 datasets were sourced from the gene expression omnibus (GEO) database. An integrated analysis of the datasets for both diseases identified biomarkers and thoroughly examined their biological roles and molecular mechanisms using differential expression analysis (DEA), weighted gene co‐expression network analysis (WGCNA), gene enrichment analysis, machine learning, microRNA (miRNA) and transcription factor analyses, immune infiltration analyses, and single‐cell transcriptome analysis. Concurrently, molecular docking was used to forecast potential drugs targeting these biomarkers. Finally, reverse transcription quantitative real‐time polymerase chain reaction (RT‐qPCR) was performed in human trabecular meshwork stem cells to validate the five identified biomarkers. The 10 key genes identified through DEA and WGCNA were predominantly involved in immune, inflammatory, and autophagy pathways. Additionally, machine learning identified five biomarkers, and we established associated transcription factors and miRNA regulatory networks. Immune infiltration analysis indicated an elevated presence of immune cells, including macrophages, T cells, and B cells, in both conditions. Furthermore, the DSigDB database yielded 10 potential therapeutic agents, three of which showed strong binding potential to the biomarkers via molecular docking. The RT‐qPCR results confirmed the trend in gene expression. This study uncovered a new link between SLE and primary open‐angle glaucoma, identifying biomarkers and mechanisms of immunopathogenesis for future research and treatment strategies.

Regulation of autophagy by ST3GAL2-mediated α2-3 sialylated glycosphingolipids in hepatic encephalopathy

In neurological diseases, the regulation of autophagy plays a crucial role in their pathology, particularly the relationship between autophagy and hepatic encephalopathy (HE) which merits detailed investigation. Glycosphingolipids are abundant and broadly functional in the nervous system and are closely associated with autophagy. However, the specific link and mechanisms between glycosphingolipids and autophagy in HE remain unclear. This study aims to explore the impact of glycosphingolipid changes on the autophagy in HE and its potential mechanisms. Utilizing lectin microarrays, we observed elevated expression levels of α2-3 sialylated glycosphingolipid in the brain tissue of HBV transgenic mice and ammonia-induced astrocyte models, suggesting that the increase in α2-3 sialylated glycosphingolipid is related to HE. Further research revealed that the increased expression of α2-3 sialylated glycosphingolipid, mediated by ST3GAL2, affects autophagy by regulating the autophagy initiation complex Vps34-Beclin-1. In summary, our research not only comprehensively reveals the changes in brain glycosphingolipid during HBV-related HE but also elucidates the interactions and regulatory mechanisms between α2-3 sialylated glycosphingolipid and autophagy. This study provides a new perspective on understanding the pathogenesis of HE and offers novel theories and targets for future research and treatment strategies.

The Kynurenine Pathway in Gut Permeability and Inflammation.

The gut-brain axis (GBA) is a crucial communication network linking the gastrointestinal (GI) tract and the central nervous system (CNS). The gut microbiota significantly influences metabolic, immune, and neural functions by generating a diverse array of bioactive compounds that modulate brain function and maintain homeostasis. A pivotal mechanism in this communication is the kynurenine pathway, which metabolises tryptophan into various derivatives, including neuroactive and neurotoxic compounds. Alterations in gut microbiota composition can increase gut permeability, triggering inflammation and neuroinflammation, and contributing to neuropsychiatric disorders. This review elucidates the mechanisms by which changes in gut permeability may lead to systemic inflammation and neuroinflammation, with a focus on the kynurenine pathway. We explore how probiotics can modulate the kynurenine pathway and reduce neuroinflammation, highlighting their potential as therapeutic interventions for neuropsychiatric disorders. The review integrates experimental data, discusses the balance between neurotoxic and neuroprotective kynurenine metabolites, and examines the role of probiotics in regulating inflammation, cognitive development, and gut-brain axis functions. The insights provided aim to guide future research and therapeutic strategies for mitigating GI complaints and their neurological consequences.

Being old is like…: perceptions of aging among healthcare profession students

BackgroundThis research explores the perspectives and attitudes of university students in health sciences towards aging and older adults. Given the intricate interplay of factors influencing attitudes toward aging, coupled with the demographic shift in Turkey from a youthful to an aging population, the study aims to discern how a cohort of university students perceives the aging process.MethodsEmploying a mixed-methods research strategy, which enhances the depth of data interpretation, the study utilized a questionnaire for quantitative data collection. Additionally, qualitative insights were gathered through a metaphor stem-completion item appended to the questionnaire and semi-structured interviews with students. The participants were selected from the Health Sciences Faculty and School of Medicine at a Turkish university.ResultsThe study revealed that participating students generally hold positive attitudes and demonstrate respect towards older adults. However, they also associate old age with negative aspects such as loss of autonomy and a constant need for assistance. Furthermore, older individuals are perceived as emotionally challenging and challenging to work with, irrespective of the nature and duration of interactions during their academic programs. These findings suggest a potential pathologizing perspective towards aging adults among health science students, who are prospective health professionals.ConclusionsThis paper discusses the implications of the study and offers insights for program coordinators, curriculum designers, and faculty members in health sciences. The results underscore the necessity for a heightened emphasis on gerontology-related subjects within health science curricula. This emphasis is crucial for cultivating a comprehensive understanding among students of the social, psychological, cognitive, and biological changes associated with aging.

Fine-tuning plant valuable secondary metabolite biosynthesis via small RNA manipulation: strategies and potential.

Plants produce secondary metabolites that serve various functions, including defense against biotic and abiotic stimuli. Many of these secondary metabolites possess valuable applications in diverse fields, including medicine, cosmetic, agriculture, and food and beverage industries, exhibiting their importance in both plant biology and various human needs. Small RNAs (sRNA), such as microRNA (miRNA) and small interfering RNA (siRNA), have been shown to play significant roles in regulating the metabolic pathways post-transcriptionally by targeting specific key genes and transcription factors, thus offering a promising tool for enhancing plant secondary metabolite biosynthesis. In this review, we summarize current approaches for manipulating sRNAs to regulate secondary metabolite biosynthesis in plants. We provide an overview of the latest research strategies for sRNA manipulation across diverse plant species, including the identification of potential sRNAs involved in secondary metabolite biosynthesis in non-model plants. We also highlight the potential future research directions, focusing on the manipulation of sRNAs to produce high-value compounds with applications in pharmaceuticals, nutraceuticals, agriculture, cosmetics, and other industries. By exploring these advanced techniques, we aim to unlock new potentials for biotechnological applications, contributing to the production of high-value plant-derived products.