The influence of post-heat-treatment on the resulting microstructure and room-temperature fracture toughness (KIC) of Inconel 718 fabricated by laser solid forming (LSF), a kind of laser based directed-energy-deposition additive manufacturing technologies, is investigated. Detailed microstructure characterization was performed on as-fabricated and heat-treated samples using direct aging (DA), solution treatment plus aging (STA), homogenization plus STA (HSTA), respectively. The results indicate that as-fabricated sample mainly consists of γ columnar dendrites and a small quantity of (γ+Laves) eutectics in the interdendritic areas. After DA post-heat-treatment, the nonuniform γ′′/γ′ precipitates exist around Laves phases. After STA post-heat-treatment, the short-acicular δ-phases precipitate around/in the Laves phases, the micro-segregation reduces and the distribution of γ′′/γ′ precipitates in the dendrite arm is almost homogeneous. After HSTA post-heat-treatment, Laves phase mostly disappears, micro-segregation completely removes and the γ′′/γ′ precipitates (~30 nm) are distributed in bimodal recrystallized grains. The results of KIC testing indicate that as-fabricated sample possesses the lowest KIC mainly due to its lowest elastic modulus and yield strength. However, the KIC of DA sample (~89.8 MPam) is close to that of as-fabricated sample, mainly due to its low ductility and strain-hardening exponent. But, the KIC of STA and HSTA samples increases by ~55.8% and 90.8% compared with as-fabricated sample respectively, mainly because of the better performance in strength–plasticity of HSTA sample (~1114 MPa, ~26.1%). Particularly, the KIC of HSTA sample with bimodal grains is ~60 MPam higher than the lower limit of wrought IN718 (AMS 5662) and reaches ~164.1 MPam. There exists significant difference in the KIC fractographs of as-fabricated and heat-treated samples. Overall, this research illustrates that an appropriate post-heat-treatment possesses obvious toughening effect on the LSF Inconel 718 alloy.