Stimulus Area Research Articles

How dogs scan familiar and inverted faces: an eye movement study

Faces play an important role in communication and identity recognition in social animals. Domestic dogs often respond to human facial cues, but their face processing is weakly understood. In this study, facial inversion effect (deficits in face processing when the image is turned upside down) and responses to personal familiarity were tested using eye movement tracking. A total of 23 pet dogs and eight kennel dogs were compared to establish the effects of life experiences on their scanning behavior. All dogs preferred conspecific faces and showed great interest in the eye area, suggesting that they perceived images representing faces. Dogs fixated at the upright faces as long as the inverted faces, but the eye area of upright faces gathered longer total duration and greater relative fixation duration than the eye area of inverted stimuli, regardless of the species (dog or human) shown in the image. Personally, familiar faces and eyes attracted more fixations than the strange ones, suggesting that dogs are likely to recognize conspecific and human faces in photographs. The results imply that face scanning in dogs is guided not only by the physical properties of images, but also by semantic factors. In conclusion, in a free-viewing task, dogs seem to target their fixations at naturally salient and familiar items. Facial images were generally more attractive for pet dogs than kennel dogs, but living environment did not affect conspecific preference or inversion and familiarity responses, suggesting that the basic mechanisms of face processing in dogs could be hardwired or might develop under limited exposure.

The shape of motion perception: Global pooling of transformational apparent motion

Transformational apparent motion (TAM) is a visual phenomenon highlighting the utility of form information in motion processing. In TAM, smooth apparent motion is perceived when shapes in certain spatiotemporal arrangements change. It has been argued that TAM relies on a separate high-level form-motion system. Few studies have, however, systematically examined how TAM relates to conventional low-level motion-energy systems. To this end, we report a series of experiments showing that, like conventional motion stimuli, multiple TAM signals can combine into a global motion percept. We show that, contrary to previous claims, TAM does not require selective attention, and instead, multiple TAM signals can be simultaneously combined with coherence thresholds reflecting integration across the entire stimulus area. This system is relatively weak, less tolerant to noise, and easily overridden when motion energy cues are sufficiently strong. We conclude that TAM arises from high-level form-motion information that enters the motion system by, at least, the stage of global motion pooling.

Sexual Self-Regulation and Cognitive Absorption as Factors of Sexual Response Toward Virtual Characters

The eye movements and penile responses of 20 male participants were recorded while they were immersed with virtual sexual stimuli. These participants were divided into two groups according to their capacity to focus their attention in immersion (high and low focus). In order to understand sexual self-regulation better, we subjected participants to three experimental conditions: (a) immersion with a preferred sexual stimulus, without sexual inhibition; (b) immersion with a preferred sexual stimulus, with sexual inhibition; and (c) immersion with a neutral stimulus. A significant difference was observed between the effects of each condition on erectile response and scanpath. The groups differed on self-regulation of their erectile responses and on their scanpath patterns. High focus participants had more difficulties than low focus participants with inhibiting their sexual responses and displayed less scattered eye movement trajectories over the critical areas of the virtual sexual stimuli. Results are interpreted in terms of sexual self-regulation and cognitive absorption in virtual immersion. In addition, the use of validated virtual sexual stimuli is presented as a methodological improvement over static and moving pictures, since it paves the way for the study of the role of social interaction in an ecologically valid and well-controlled way.

Faces in Motion: Selectivity of Macaque and Human Face Processing Areas for Dynamic Stimuli

Face recognition mechanisms need to extract information from static and dynamic faces. It has been hypothesized that the analysis of dynamic face attributes is performed by different face areas than the analysis of static facial attributes. To date, there is no evidence for such a division of labor in macaque monkeys. We used fMRI to determine specializations of macaque face areas for motion. Face areas in the fundus of the superior temporal sulcus responded to general object motion; face areas outside of the superior temporal sulcus fundus responded more to facial motion than general object motion. Thus, the macaque face-processing system exhibits regional specialization for facial motion. Human face areas, processing the same stimuli, exhibited specializations for facial motion as well. Yet the spatial patterns of facial motion selectivity differed across species, suggesting that facial dynamics are analyzed differently in humans and macaques.

Subjects’ hypnotizability level affects somatosensory evoked potentials to non-painful and painful stimuli

ObjectiveWe evaluated the working hypothesis that the EEG activity associated to non-painful and painful stimuli in condition of waking state (no hypnotic procedure) was related to the hypnotizability level. MethodsHypnotizability level was measured in 16 healthy subjects through the Italian version of the Stanford Hypnotic Susceptibility Scale (SHSS, score: 0–12). EEG data (56 electrodes) were recorded during non-painful and painful electrical stimuli applied to the left index finger. Cortical activity (vertex N1–P2 complex) was compared in subjects with low hypnotizability level (N=8, SHSS:0–6) vs. subjects with high hypnotizability level (N=8, SHSS:7–12). ResultsThe amplitude of the N1–P2 complex was lower in the High-hypnotizability compared to the Low-hypnotizability group over primary sensorimotor cortex (C3 and C4 electrodes) and centro-parietal midline areas (Cz and Pz electrodes) for non-painful and painful stimuli. The SHSS showed a statistically significant negative correlation with the vertex N1–P2 complex at C3 and Cz (r=−0.5, p<0.05) electrodes for non-painful stimuli. ConclusionCompared to the Low-hypnotizability subjects, High-hypnotizability subjects showed a reduced cortical activity related to non-painful and painful stimuli. SignificanceThe results suggest a relationship between hypnotizability and cortical activity related to non-painful and painful stimuli in the condition of waking state (no hypnotic effect).

Brief Report: Examining Driving Behavior in Young Adults with High Functioning Autism Spectrum Disorders: A Pilot Study Using a Driving Simulation Paradigm

Although it is speculated that impairments associated with autism spectrum disorder (ASD) will adversely affect driving performance, little is known about the actual extent and nature of the presumed deficits. Ten males (18-24 years of age) with a diagnosis of high functioning autism and 10 age matched community controls were recruited for a driving simulation experiment. Driving behavior, skin conductance, heart rate, and eye tracking measurements were collected. The high functioning ASD participants displayed a nominally higher and unvaried heart rate compared to controls. With added cognitive demand, they also showed a gaze pattern suggestive of a diversion of visual attention away from high stimulus areas of the roadway. This pattern deviates from what is presumed to be optimal safe driving behavior and appears worthy of further study.

Stability of simple/complex classification with contrast and extraclassical receptive field modulation in macaque V1

A key property of neurons in primary visual cortex (V1) is the distinction between simple and complex cells. Recent reports in cat visual cortex indicate the categorization of simple and complex can change depending on stimulus conditions. We investigated the stability of the simple/complex classification with changes in drive produced by either contrast or modulation by the extraclassical receptive field (eCRF). These two conditions were reported to increase the proportion of simple cells in cat cortex. The ratio of the modulation depth of the response (F1) to the elevation of response (F0) to a drifting grating (F1/F0 ratio) was used as the measure of simple/complex. The majority of V1 complex cells remained classified as complex with decreasing contrast. Near contrast threshold, an equal proportion of simple and complex cells changed their classification. The F1/F0 ratio was stable between optimal and large stimulus areas even for those neurons that showed strong eCRF suppression. There was no discernible overall effect of surrounding spatial context on the F1/F0 ratio. Simple/complex cell classification is relatively stable across a range of stimulus drives, produced by either contrast or eCRF suppression.

Differential responses in dorsal visual cortex to motion and disparity depth cues

We investigated how interactions between monocular motion parallax and binocular cues to depth vary in human motion areas for wide-field visual motion stimuli (110 × 100°). We used fMRI with an extensive 2 × 3 × 2 factorial blocked design in which we combined two types of self-motion (translational motion and translational + rotational motion), with three categories of motion inflicted by the degree of noise (self-motion, distorted self-motion, and multiple object-motion), and two different view modes of the flow patterns (stereo and synoptic viewing). Interactions between disparity and motion category revealed distinct contributions to self- and object-motion processing in 3D. For cortical areas V6 and CSv, but not the anterior part of MT+ with bilateral visual responsiveness (MT+/b), we found a disparity-dependent effect of rotational flow and noise: When self-motion perception was degraded by adding rotational flow and moderate levels of noise, the BOLD responses were reduced compared with translational self-motion alone, but this reduction was cancelled by adding stereo information which also rescued the subject's self-motion percept. At high noise levels, when the self-motion percept gave way to a swarm of moving objects, the BOLD signal strongly increased compared to self-motion in areas MT+/b and V6, but only for stereo in the latter. BOLD response did not increase for either view mode in CSv. These different response patterns indicate different contributions of areas V6, MT+/b, and CSv to the processing of self-motion perception and the processing of multiple independent motions.

Stimulus Point Distribution in Deep or Superficial Peroneal Nerve for Treatment of Ankle Spasticity

To develop effective electrical stimulation treatment to reduce spasticity, we examined the optimal stimulus point of the common peroneal nerve. The locations of selective stimulus points for the deep peroneal nerve or superficial peroneal nerve fiber were examined in 25 healthy subjects in both legs (50 legs) using the ratio of the tibialis anterior (TA) to the peroneus longus (PL) M-wave amplitude (TA/PL ratio). In addition, we measured reciprocal Ia inhibition in ten healthy subjects. The amount of inhibition was determined from short-latency suppression of the soleus (Sol) H-reflex by conditioning stimuli to the deep or superficial peroneal nerve. The paired t-test was used for statistical analysis. The mean TA/PL ratio during deep peroneal nerve stimulation was significantly different from superficial peroneal nerve stimulation (p < 0.001). The mean stimulus point for the deep peroneal nerve was located 7 ± 5 mm distal and 3 ± 6 mm anterior from the distal edges of the head of fibula and was markedly different from the stimulus point for the superficial peroneal nerve (20 ± 7 mm distal and 12 ± 8 mm posterior). During deep peroneal nerve stimulation, the mean conditioned H-reflex was depressed to 83.8 ± 10.7% of the unconditioned value of the H-reflex. In contrast, during superficial peroneal nerve stimulation, the mean conditioned H-reflex increased to 105.3 ± 5.2%. These values were significantly different (p < 0.001). In the present study, we revealed a stimulus area of the deep peroneal nerve. Also, we observed the inhibitory effects of stimulation upon the deep peroneal nerve at individual stimulus point. Our results appear to indicate that localized stimulation of the deep peroneal nerve is more useful for the reduction of ankle spasticity.

Effect of light source size on discomfort glare thresholds

AbstractPurpose Bright light sources, such as car headlamps or residential street lights, can cause visual discomfort. The origins of discomfort glare (DG) or the factors that contribute to it remain poorly understood, even after 50 years of multidisciplinary research [American Journal of Ophthalmology, 153(4):587‐593(2012)]. This study examined the effect of the light‐source intensity, size, surround luminance and pupil size on DG thresholds.Methods The pupil was measured in real time to calculate retinal illuminance, a more pertinent parameter in studies of DG. DG thresholds were estimated with a staircase procedure: the retinal illuminance was increased or decreased based on whether the participant indicated the absence or presence of DG, respectively. The subjects were required to view a source of light presented against a simulated residential street background. The size of the disc source was selected randomly under computer control. Five different source sizes were used to produce a 40 fold variation in stimulus area. The light source behind the aperture disc was designed to produce uniform flashes of light of varying intensity, while the surround light level remained unchanged.Results It was found that at the threshold for DG, the retinal illuminance is approximately constant and independent of source size. On the other hand, pupil plane illuminance increases as source size increases.Conclusion These findings suggest that DG depends mostly on localised retinal illuminance, rather than the total amount of light entering the eye. DG therefore appears to be largely related to saturation of photoreceptor signals. The results also suggest that higher levels of street illumination can be achieved by increasing the size of light source without causing any increase in DG.

Perception of heading speed from radial flow depends on visual field

We investigated the effects that the visual field has on the perception of heading speed. The stimulus was a radial flow pattern simulating a translational motion through a cylindrical tunnel. Observers evaluated the perception of heading speed by using a temporal two-alternative forced choice (2AFC) staircase method. In the first experiment, we manipulated the stimulus area by cutting the visual field along the longitudinal direction. The results showed that the perceived heading speed increases with the stimulus area. In the second experiment, we manipulated both the stimulus area and the eccentricity by cutting the visual field along the longitudinal direction. The results showed that the perception of heading speed increases when the stimulus occupies a large portion of the peripheral visual field. These findings suggest that the effect of eccentricity is a consequence of an incorrect translation of two-dimensional visual information into three-dimensional scaling.

Assessment of a Neurophysiological Model of the Mandibular Branch of the Facial Nerve in Rats by Electromyography

Our objective was to develop an experimental model for the noninvasive and objective evaluation of facial nerve regeneration in rats using a motor nerve conduction test (electromyography). Twenty-two rats were submitted to neurophysiological evaluation using motor nerve conduction of the mandibular branch of the facial nerve to obtain the compound muscle action potentials (CMAPs). To record the CMAPs, we used two needle electrodes that were inserted into the lower lip muscle of the rat. A supramaximal electrical stimulus was applied, and the values of CMAP latency, amplitude, length, area, and stimulus intensity obtained from each side were compared by use of the Wilcoxon test. There was no significant difference (all p > 0.05) in latency, amplitude, duration, area, or intensity of stimuli between the two sides. The amplitudes ranged between 1.61 and 8.30 mV, the latencies between 1.03 and 1.97 ms, and the stimulus intensities between 1.50 and 2.90 mA. This is a noninvasive, easy, and highly reproducible method that contributes to an improvement of the techniques previously described and may contribute to future studies of the degeneration and regeneration of the facial nerve.

Closed-Loop Measurements of Iso-Response Stimuli Reveal Dynamic Nonlinear Stimulus Integration in the Retina

Neurons often integrate information from multiple parallel signaling streams. How a neuron combines these inputs largely determines its computational role in signal processing. Experimental assessment of neuronal signal integration, however, is often confounded by cell-intrinsic nonlinear processes that arise after signal integration has taken place. To overcome this problem and determine how ganglion cells in the salamander retina integrate visual contrast over space, we used automated online analysis of recorded spike trains and closed-loop control of the visual stimuli to identify different stimulus patterns that give the same neuronal response. These iso-response stimuli revealed a threshold-quadratic transformation as a fundamental nonlinearity within the receptive field center. Moreover, for a subset of ganglion cells, the method revealed an additional dynamic nonlinearity that renders these cells particularly sensitive to spatially homogeneous stimuli. This function is shown to arise from a local inhibition-mediated dynamic gain control mechanism.

Cross-language specialization in phonetic processing: English and Hindi perception of /w/-/v/ speech and nonspeech

This study examined the perceptual specialization for native-language speech sounds, by comparing native Hindi and English speakers in their perception of a graded set of English /w/-/v/ stimuli that varied in similarity to natural speech. The results demonstrated that language experience does not affect general auditory processes for these types of sounds; there were strong cross-language differences for speech stimuli, and none for stimuli that were nonspeech. However, the cross-language differences extended into a gray area of speech-like stimuli that were difficult to classify, suggesting that the specialization occurred in phonetic processing prior to categorization.

Experimental evidence for olfactory predator recognition in wild mouse lemurs

Although primates have remarkable olfactory capabilities, their ability for olfactory predator recognition is still understudied. We investigated this cognitive ability in wild gray and golden-brown mouse lemurs (Microcebus murinus and M. ravelobensis) that were confronted with four different olfactory stimuli, derived from two Malagasy predators (fossa and barn owl) and two local nonpredator species (brown lemur and sifaka). The predator response was tested (1) in a systematic cage setup and (2) in a two-way choice experiment with two Sherman traps on platforms in the forest (stimulus trap vs. nonstimulus trap). For part 1, the study animals were housed in cages during habituation and 5 days of experiments. One stimulus was tested per night and was presented underneath a drinking bottle. The changes in the time spent close to the stimulus and the drinking time at the bottle were used as indicators of predator recognition. A timidity score was established by classifying the strength of the antipredator response during the experiment. The study animals spent significantly less time drinking and less time in the stimulus area when confronted with fossa odor compared with the other stimuli. The timidity score was significantly higher during the fossa stimulus compared with the nonpredator and the control stimuli. The two-way choice experiments revealed a complete avoidance of the fossa odor, which was not found with the other stimuli. Thus, wild mouse lemurs showed clear signs of olfactory predator recognition in the case of the fossa in both experiments, but no signs of avoidance to the other presented stimuli. The lack of owl avoidance may be explained by less or no aversive metabolites in the owl stimulus or by lower significance for olfactory recognition of aerial predators. Furthermore, the results showed slight differences between the two mouse lemur species that may be linked to differences in their ecology.

Visual and Haptic Size Perception of Stimulus Length, Area, and Volume

Visual and haptic size perception of length (1D), area (2D), and volume (3D) was investigated with the magnitude estimation method. In the visual condition, subjects viewed lines, outlined circles or shaded spheres presented on LCD monitor, and assigned numbers to the perceived length, area or volume of the stimuli. In the haptic condition, subjects grasped rectilinear, cylindrical or spherical blocks which were occluded from subjects' view and assigned numbers. For both conditions, the data were well described by power functions of length, area or volume of the stimuli, and exponents of the power functions became smaller with increasing dimensionality. For the visual (but not haptic) condition, when the data were described by power functions of length (for 1D) or diameter (for 2D and 3D), exponents were approximately constant at around1.0. This suggests that the determinant of the visual size perception for 2D and 3D stimuli may be length rather than area or volume. The critical role of 1D cue for the visual size perception may cause elongation bias, as termed by Holmberg (1975).

Flashlight – Recording information acquisition online

A flashlight enables a person to see part of the world in the dark. As a person directs a flashlight beam to certain places in the environment, it serves as a manifestation of their attention, interest and focus. In this paper we introduce Flashlight, an open-source (free) web-based software package that can be used to collect continuous and non-obtrusive measures of users’ information acquisition behavior. Flashlight offers a cost effective and rapid way to collect data on how long and how often a participant reviews information in different areas of visual stimuli. It provides the functionality of other open source process tracing tools, like MouselabWeb, and adds the capability to present any static visual stimulus. We report the results from three different types of stimuli presented with both the Flashlight tool and a traditional eye-tracker. We found no differences measuring simple outcome data (e.g., choices in gambles or performance on algebraic tasks) between the two methods. However, due to the nature of the more complicated information acquisition, task completion takes longer with Flashlight than with an eye-tracking system. Other differences and commonalities between the two recording methods are reported and discussed. Additionally we provide detailed instructions on the installation and setup of Flashlight, the construction of stimuli, and the analysis of collected data.

Pressure thresholds of the human foot: measurement reliability and effects of stimulus characteristics

Information related to reliable values of discomfort thresholds can help to improve the designs of various products. This study aimed to investigate the measurement reliabilities associated with pressure thresholds, while determining the effects of stimulus characteristics (stimulus area, indentation speed) of the human foot. An indentation apparatus was used with four sizes of indentation probes and three indentation speeds. In total, 13 locations on the right foot of 10 male and 10 female participants were tested to determine the pressure discomfort thresholds (PDT) and pressure pain thresholds (PPT). Results show that the tests had very good measurement reliability with intra-class correlations (ICC) greater than 0.8 for the PPT measurements and acceptable reliability (most ICC > 0.75, with a few between 0.5 and 0.75) for the PDT measurements, demonstrating that participants are capable of judging their pain and discomfort thresholds. Pressure sensitivity differs across locations of the foot, with the medial plantar arch of the foot being the most sensitive, followed by the dorsal surface of the foot. The heel area was the least sensitive. PPT and PDT are dependent on the stimulus characteristics of the area and the speed of indentation. A smaller area has a higher PPT and PDT, indicating significant effects of spatial summation. The increase of PDT and PPT at higher speeds may be partially explained by the increase in stiffness because foot tissue exhibits viscoelastic properties. The findings can have a significant impact on the design of footwear and other accessories for improved foot health and comfort. Statement of Relevance: This study investigated the threshold measurement reliability while determining the pressure sensitivity on the surface of the foot with varying stimulus characteristics. The findings may be very useful in the design of footwear and other accessories for improved comfort and reduced injuries.

Assessing Signal-Driven Mechanisms in Neonates: Brain Responses to Temporally and Spectrally Different Sounds

Past studies have found that, in adults, the acoustic properties of sound signals (such as fast versus slow temporal features) differentially activate the left and right hemispheres, and some have hypothesized that left-lateralization for speech processing may follow from left-lateralization to rapidly changing signals. Here, we tested whether newborns’ brains show some evidence of signal-specific lateralization responses using near-infrared spectroscopy (NIRS) and auditory stimuli that elicits lateralized responses in adults, composed of segments that vary in duration and spectral diversity. We found significantly greater bilateral responses of oxygenated hemoglobin (oxy-Hb) in the temporal areas for stimuli with a minimum segment duration of 21 ms, than stimuli with a minimum segment duration of 667 ms. However, we found no evidence for hemispheric asymmetries dependent on the stimulus characteristics. We hypothesize that acoustic-based functional brain asymmetries may develop throughout early infancy, and discuss their possible relationship with brain asymmetries for language.

Effects of sweep VEP parameters on visual acuity and contrast thresholds in children and adults

There are many parameters that may impact the thresholds obtained with sweep visually evoked potentials (sVEP), yet a number of these parameters have not been systematically studied, and there is no recognised standard for sVEP recording. In this study, the effects of electrode placement, temporal frequency, sweep direction, presence of a fixation target, stimulus area, and sweep duration on visual acuity (VA) and contrast thresholds of the sVEP were investigated. Additionally, the effect of these parameters on the number of viable threshold readings obtained from five active electrodes was investigated. Participants were six children (aged 6-8 years) and six adults (aged 17-30 years) with normal vision. Binocular sVEP VA and contrast thresholds were measured for two electrode placements (ISCEV and PowerDiva) of five active electrodes, three temporal frequencies (6, 7.5, and 10 Hz), two sweep directions (low to high and high to low), presence or absence of a fixation target, three stimulus areas, and three sweep durations. There were differences between adults and children with respect to visual acuity, the adults having better VA than the children (p = 0.033 in experiment 2). Overall, there were more viable readings at 7.5 Hz than at either 10 or 6 Hz (p = 0.0014 for VA and 0.001 for contrast thresholds). The adults performed better (in terms of viable readings) with the fixation target than without it (p = 0.04). The smallest stimulus size used gave rise to fewer viable readings in both adults and children (p = 0.022 for VA and 0.0001 for contrast thresholds). The other parameters (electrode placement, sweep direction and sweep duration) did not give rise to significant differences. A temporal frequency of 7.5 Hz, a stimulus area of 4° or larger for VA and 10° or larger for contrast thresholds, and the use of a fixation target gave more viable readings, and may be indicated for future application. Consideration of the number of viable readings showed more differences between parameters than the actual thresholds, and it is suggested that more readings presumably would yield more reliable threshold measurements.