Background: In India secondary-level public health-care facilities (HCF)-District Hospitals, are not equipped either with a microbiological laboratory or a robust Infection control program. The present study was initiated to investigate the epidemiology of neonatal septicaemia in SNCUs (Special new-born care units) situated at various District Hospitals of Chhattisgarh (a state in Central India) in terms of Blood Stream Infection (BSI) rates, pathogen profile and their antimicrobial sensitivity patterns. Methods & Materials: The study was conducted from October 2017 through June 2019 at the Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Raipur, a tertiary level healthcare teaching institute in collaboration with 23 SNCUs. Paired blood samples (0.5–1.0 ml) were collected in paediatric aerobic blood culture bottles (BacT/ALERT PF Plus, BioMerieux Inc. Durham, NC, USA) and transported to the laboratory, where culture and identification was performed by automated ID and AST system (Vitek 2 ID/AST system, BioMerieux Inc. Durham, USA) and by manual methods using standard laboratory protocol. Results: Paired blood cultures were received from 2843 neonates; out of which 1645 (58%) were sterile, 388 (13.6%) grew contaminants and 810 grew pathogens, indicating BSI rate of 28.4%. Gram negative bacilli were the most commonly encountered pathogens (382/810, 47.2%), with more than half being multi drug resistant (211/382, 55.2%). Klebsiella pneumoniae was the commonest isolate (n = 253). Amongst the gram- positive cocci (253/810, 31.2%) coagulase negative staphylococcus (CoNS) was commonest. Only 17 (6.7%) Methicillin Resistant Staphylococcus aureus and one Streptococcus agalactiae were isolated. Candida spp. accounted for 21.6% (175/810) pathogen, predominated by non-albicans candida (161/175). 18% of the Candida spp. were resistant to Fluconazole, while 1.2% were MDR. A fall in NMR (neonatal mortality rate) and antibiotic usage rate was observed in almost all the units after the microbiology laboratory support was initiated. Conclusion: The secondary-level public health-care facilities have a very high rate of laboratory proven neonatal septicemia with an alarmingly high AMR (Antimicrobial resistance) rate, both among bacterial and fungal isolates. This warrants urgent intervention for reducing neonatal septicaemia by microbiology laboratory support, optimising infection control practices and implementing AMSP (antimicrobial stewardship program).
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