The objective of the study is to examine the in vivo effect of star fruit juice at different storage conditions on the activity of alkaline phosphates in female Sprague Dawley (SD) rats. A total of 20 healthy female rats weighing 180-200g were used for the experiment. All animals were divided into four groups with five animals per group (n=5). First control was served as control group. Second, third and forth groups were orally treated with a single dose daily of freshly prepared star fruit juice, juice stored for 1-hour and juice stored for 3-hour, respectively for 14 days. All animals were sacrificed at day-15 and various organs such as liver, kidney and heart were removed. All organs were homogenised and centrifuged to prepare cytosolic fraction as a source of alkaline phosphatase enzyme. Protein content of each organ was determined. p-nitrophenol phosphate was used as a substrate to determine the activity of alkaline phosphatase using spectrophotometer. All results were analysed using Dunnett’s test. Based on the results obtained, a significant increase (P<0.01) of the activity of alkaline phosphatase in rat liver was observed in all star fruit juice treatment groups when compared to control rats. In conclusion, star fruit juice at different storage times was selectively induced the activity of alkaline phosphatase in rat liver but not in the heart and kidney.
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