Staphylea Pinnata Research Articles

Health-Promoting Properties of Natural Flavonol Glycosides Isolated from Staphylea pinnata L.

Staphylea, also called bladdernuts, is a genus of plants belonging to the family Staphyleaceae, widespread in tropical or temperate climates of America, Europe, and the Far East. Staphylea spp. produce bioactive metabolites with antioxidant properties, including polyphenols which have not been completely investigated for their phytotherapeutic potential, even though they have a long history of use for food. Here, we report the isolation of six flavonol glycosides from the hydroalcoholic extract of aerial parts of Staphylea pinnata L., collected in Italy, using a solid-phase extraction technique. They were identified using spectroscopic, spectrometric, and optical methods as three quercetin and three isorhamnetin glycosides. Among the flavonol glycosides isolated, isoquercetin and quercetin malonyl glucoside showed powerful antioxidant, antimicrobial, and wound healing promoting activity and thus are valuable as antiaging ingredients for cosmeceutical applications and for therapeutic applications in skin wound repair.

Isolation, identification and quantification of flavonoids from the flowers of <i>Staphylea pinnata</i> L.

Introduction. Search for new plant species containing biologically active substances (hereinafter – BAS) is one of the leading tasks of pharmacognosy as a science. The search for flavonoid glycosides in plant raw materials is especially relevant, since they have anti-inflammatory, antioxidant, immunostimulating, as well as weak fungicidal and bacteriostatic action. Staphylea pinnata L. is an endemic plant of the Caucasus, cultivated not only in Georgia, but also in the Russian Federation in the Northern and Northwestern Caucasus. In foreign literature there are studies of antioxidant, antiproliferative and cytotoxic activity of leaf extracts of several species of S. pinnata L., as well as inhibitory activity of COX-1, COX-2 and LTB4 formation. Meanwhile, no serious Russian-language scientific studies on either the chemical composition or pharmacological action of generative organs of S. pinnata were found in the literature. This work is part of a comprehensive phytochemical study of S. pinnata. The aim of the work is to study the qualitative and quantitative composition of flavonoids in the studied object.Aim. To isolate, identify and quantify flavonoids in flowers and buds of Staphylea pinnata L.Materials and methods. Alcohol-water extracts from dried generative organs of the studied plant were used as analyzed solutions. Solutions were analyzed on a spectrophotometer SF-2000 (LLC "OKB Spectr", Russia) after sample preparation with aluminum chloride and on an HPLC Nexera-i LC-2040 (Shimadzu Corporation, Japan) equipped with a column and sample thermostat, degasser and autosampler using an individually selected elution gradient of the mobile phase (0.1 % orthophosphoric acid/acetonitrile solution). The primary data were processed using LabSolutions Single LC software (Shimadzu Corporation, Japan). Compounds from the flavonoid group were identified by retention times. Detection was carried out using a UV detector with an absorption wavelength of 365 ± 2 nm.Result and discussion. Alcohol-water extracts from flowers and buds of S. pinnata L. were obtained. Quantitative evaluation by spectrophotometry for flavonoid content was carried out. A gradient elution mode for HPLC was selected for simultaneous determination of 7 flavonoid glycosides. These chromatographic conditions allowed the identification and quantification of astragaline, cynaroside, cosmosiin, narcissin and rutin in flowers and buds of Staphylea pinnata L. Flavonoid glycosides: raponticin and kaempferol were not detected.Conclusion. Flavonoid glycosides were isolated from the generative organs of S. pinnata L., a technique for quantitative determination of flavonoid glycosides in alcohol-water extracts was developed, astragalin, cynaroside, cosmosiin, narcissin and rutin were detected and quantified.

Некоторые морфобиологические особенности клекачки перистой при интродукции в Главном ботаническом саду им. Н.В. Цицина РАН

This article discusses the possibility of seed and vegetative reproduction of Staphylea pinnata L., growth and development of the plant in the conditions of the Main Botanical Garden in Moscow. Keywords: STAPHYLEA PINNATA, BLADDERNUT, SEED AND VEGETATIVE REPRODUCTION, GREEN CUTTINGS, GROWTH AND DEVELOPMENT

Особенности зимней транспирации клекачки перистой (Staphylea pinnata L.) и некоторых других кустарников в условиях Главного ботанического сада им. Н.В. Цицина (Москва)

A comparative study of the winter transpiration of annual shoots of shrubs of different winter hardiness was carried out: Staphylea pinnata L., Viburnum opulus L., Sambucus racemosa L., Sambucus cannadensis L., grown in the Main Botanical Garden named by N.V. Tsitsin Russian Academy of Science in Moscow. The intensity of diurnal transpiration was higher in non-frost-resistant shrubs. Keywords: WINTER TRANSPIRATION, WINTER HARDINESS, MAIN BOTANICAL GARDEN, STAPHYLEA PINNATA L, VIBURNUM OPULUS L., SAMBUCUS RACEMOSA L., SAMBUCUS CANNADENSIS L.

New and additional information on the flora of the Middle Dnieper

The article provides an annotated list of new floristic finds in the flora of the Middle Dnieper (on territory of Cherkasy region, as well as adjacent districts of Kyiv and Poltava regions), both aboriginal rare and alien species, including “runaways from culture”. In some cases, notes are given on the taxonomic status, features of the natural range, ancient indications of the localities of certain species in the region. For the first time, 31 new taxa were introduced for the regional flora of Cherkasy region, including 8 native and 23 alien. The most valuable is the information about rare aboriginal plants, in particular Asparagus tenuifolius, Euphorbia salicifolia, Polygonum patulum, Rosa andegavensis, etc. In the Uman district of Cherkasy region, the location of three species of flora that were considered disappeared in the region: Pentanema oculus-christi, Salvia dumetorum and Staphylea pinnata. For once, 3 new alien species have been introduced for the flora of Poltava region: Anthriscus caucalis, Lonicera × notha and L. ruprechtiana. The research revealed new locations of invasively active foreign plants, including ergasiophygophytes: Phytolacca acinosa, Silphium perfoliatum, Vitis riparia, Zizania latifolia etc., and quarantine xenophytes: Cenchrus longispinus and Eriochloa villosa. Progressive distribution of xenophytes with primary sub-Mediterranean and Central Asian habitats has been noted. Population growing of foreign plants emphasizes the existing negative trend of adventitia of the flora.The issues of naturalization of ergasiophytes are discussed, in particular the presence of mass selfseeding in the centers of their primary introduction, which is the impetus for their further spread. Provided information on spontaneous seed reproduction of species such as: Castanea sativa, Catalpa ovata, C. speciosa, Pilosella aurantiaca, Verbesina encelioides, Viola sororia and other cultivated plants. The above and similar information is an considerable element of comprehensive floral research.

Introduced tree and shrub species for sustainable landscape compositions in the urban conditions

Low temperature is оne оf the mоst significаnt limiting factоrs determining the distribution of tree species. Evaluation of the degree of freezing of tree and shrub species is of great importance for decorative gardening. Plants freeze a lot not only in harsh winters, but also during prolonged thaws with a subsequent drop in temperature, which negatively affects their ornamentation. In this regard, one of the main ways to preserve the ornamental and plant biodiversity of city parks in a temperate continental climate is to replenish existing plantings with frost-resistant tree species. It is the first study of the frost resistance of introduced trees and shrubs, which was made to isolate species adapted to the winter conditions of the temperate continental climate to form stable landscape compositions in urban parks. Based on the results of artificial freezing, species of woody plants with high frost resistance were selected: Betula kelleriana, Betula raddeana, Staphylea pinnata, Cotinus Coggygria and Pinus pallasiana. The above species of wood are recommended for sustainable landscape design in the city.

Early Floral Resources for Urban Bees From Ornamental Shrubs Ribes Aureum, Ribes Sanguineum and Staphylea Pinnata

Abstract This research aims to assess ornamental shrubs Ribes aureum, R. sanguineum and Staphylea pinnata as an early food source for urban bees. In a two-year study, the abundance and flowering pattern, nectar and pollen production, and spectrum of urban insect visitors were investigated in Lublin, SE Poland. The apiarian value of S. pinnata was studied for the first time. The studied species exhibited abundant and extended flowering, skewed inflorescence flowering pattern, and persistent flowers and inflorescences. They bloomed from late March to late May and could be considered as valuable early foraging resources for urban bees, despite the substantial differences between the species. The highest mean sugar yield and pollen yield · plant−1 (92.9 g and 44.0 g, respectively) was estimated for S. pinnata whereas the lowest ones were found in R. sanguineum (4.3 g and 1.8 g, respectively). Hymenopterans were the principal flower visitors in the studied species accounting for more than 79% of all visits.

Identification and control of endophytic bacteria during in vitro cultures of Staphylea pinnata L.

Abstract This study focused on the identification and elimination of endophytic bacterial contaminations during in vitro propagation of European bladdernut (Staphylea pinnata). Axillary shoots were propagated on Murashige and Skoog medium with 20 mg ∙ dm−3 FeEDDHA, 5 μM BA and 0.5 μM NAA at 20/18°C (day/night) and a 16-h photoperiod. Clouding by endophytic bacterial colonies was observed where shoots contacted the media. Bacteria were isolated and separated by repeated streaking as two strains. Gram staining revealed that both strains were Gram-negative. The colonies were very precisely identified as Acinetobacter johnsonii, strain ATCC 17909, and Methylobacterium rhodesianum, strain DSM 5687, using VITEK®2—a rapid bacterial identification system—and the 16S rRNA gene sequencing method. The agar disc-diffusion test proved that both bacterial strains were susceptible to 13 antibiotics (out of 25 tested), derived from the groups of fluoroquinolones, aminoglycosides and tetracyclines. Doxycycline or gentamicin (100–300 mg ∙ dm−3) was added to the S. pinnata shoot propagation medium to eliminate bacteria. Gentamicin 100 mg ∙ dm−3 showed the best effect, inhibiting the growth of endogenous bacteria (63%) when applied in the medium for 4 weeks. After the following transfer to media without antibiotics, shoots developed axillary buds and bacterial colonies were not observed.

Medicinal Remedies is Plant are Species of Staphylea pinnata L. and Family Staphyleaceae Lindl. in the Georgia (South Caucasus)

Medicinal plants of Staphylea pinnata L. Family Staphyleaceae Lindl. are in the genus Staphylea L. Species. Staphylea pinnata L. is an accepted name in Georgia and it is medicinal plants and it has vitamin A, B1, C, E. This plants it has gene 2n=26. Staphylea pinnata L. is an accepted name in Georgia and is are 1-Kakheti 320 m-618m. 2-Tbilisi 400m-769m. 3-Lower-Kartli BakMedicinal plants of Staphylea pinnata L. Family Staphyleaceae Lindl. are in the genus Staphylea L. Species. Staphylea pinnata L. is an accepted name in Georgia and it is medicinal plants and it has vitamin A, B1, C, E. This plants it has gene 2n=26. Staphylea pinnata L. is an accepted name in Georgia and is are 1-Kakheti 320 m-618m. 2-Tbilisi 400m-769m. 3-Lower-Kartli Bakuriani 534m–862m and Borjomi 788m-977m. 4-Samtskhe-Javakheti Akhaltsikhe 932m-1058m. 5-Adjara 234m-519m. Staphylea pinnata L. is a deciduous shrub growing to 4.5m. Said to taste like pistachios, the seeds are eaten by children in village Shilda. Plants for a future can not take any responsibility for any adverse effects from the use of plants. Always seek advice from a Georgia before using a plant medicinally. It is a shrub or small tree, with 5 m tall green shoots. Leaves 5-7 elongate, toothed, 5.5-11cm long. Leaves of leaves blunt 7-10mm long, 2.5-3.5mm wide. The seeds are of great size, 1-1.2cm long and 1.1cm wide. Georgian flora has species of Staphylea pinnata L. and it is an accepted scientific name is for and the Kakheti and this is the village Shilda. Georgian flora has species of Staphylea pinnata L. and it is an accepted scientific name is for and the Kakheti and this is the village Shilda. This is the plants and where my mother Tsira Sidamon-Eristavi is apartment as medicinal plants is the village of Shilda. Habitats Rich moist thickets along streams and the borders of woods.

Evaluation of bioactive constituents in European bladdernut (Staphylea pinnata L.) seed kernels

This study aimed to quantify the content of bioactive constituents of European bladdernut (Staphylea pinnata L.) seed kernels, which have been poorly examined chemically, even though they have a long history of use for food. Chlorophylls, carotenoids, tocopherols, ascorbic acid, phenolics, fatty acids, sugars and organic acids were analysed. European bladdernut kernels contain appreciable amounts of bioactive constituents. The high contents of tocopherols, carotenoids and chlorophyll make bladdernut kernels a very promising food source in comparison to other nuts. The phenolic content was lower than in other nut species. The content of total fatty acids was up to 419 g/kg fresh weight, and the ratio n-6/n-3 was 10/1. The major fatty acid was linoleic acid (45% of total fatty acids). No significant difference was found between wild and cultivated (ornamental) plants, except for shikimic acid, which was significantly higher in cultivated bladdernut. The chemical composition of the kernels makes bladdernut one of the most interesting sources of health promoting substances among seeds and nuts. The results of this study show that the utilisation of E. bladdernut seeds for food uses is worth encouraging. However, more research is needed to investigate potential antinutritive substances in bladdernut kernels.

A Fistful of Bladdernuts: The Shifting Uses of Staphylea pinnata L. as Documented by Archaeology, History, and Ethnology

An interdisciplinary approach combining archaeological, historical, and ethnological data is used in the attempt to draw a general image of the role of bladdernut (Staphylea pinnata) in past societies. The purposes encountered in this literature study extend from nutritional and medicinal uses to particular ritual/religious aspects, incorporating apotropaic and sympathetic magic, the use in grave goods, and the role of bladdernut in rosaries. In the two latter purposes, the ‘cut nose’ aspect of the seeds is suggested to be an important symbolic factor.Research into the past cultural dimensions of plants is often restricted to plants with important uses, cultivated for millennia and ever sought after, and of fundamental meaning to human subsistence and economy. This is definitely true for the main cultivated crops of the Old World, and for plants regarded essential for other (e.g. medical) reasons. Bladdernut is definitely not one of these ‘great’ useful plants. Still, this shrub has had a curious past which seemed to us worth investigating, for the beliefs and meanings that still cling to it. As we will see, new beliefs are still developing.Largely building upon the previous detailed work by the first author, the current study pursues the goal of drawing as complete a picture as possible of the cultural relevance of bladdernut in past societies. This has been done by critically evaluating the extant literature on material evidence, written historical sources, and ethnographic studies on Staphylea pinnata across Europe, and trying to suggest new interpretations for this plant. Originally given as a conference paper by the first author listed, the following article has been considerably reworked and now includes substantially more research than previously.

LAW PROTECTED PLANTS OF NATIONAL NATURE RESERVE DREVENÍK (HORNÁD BASIN) IN PROJECT LEARNING

The National nature reserve in Drevenik has an area of 101.82 ha with the highest degree of plant protection. Travertine formation is located at an altitude of 609 m a.s.l. in the eastern part of Hornad basin in East Slovakia. In the Drevenik area there are around 270,470 species and subspecies of vascular plants. Approximately 60 species are classified as endangered species of Slovakia and are classified as mountain, lowland, forest and steppe species. The plants are of phytogeographical significance, some reaching their northern limit of range in this area. Protected species of the area include (Pulsatilla slavica G. Reuss.), Alpine aster (Aster alpinus L.), Carpathian harebell (Campanula carpatica Jacq.), Pontic dragonhead (Dracocephalum austriacum L.), Hungarian iris (Iris aphylla subsp. hungarica), European columbine (Aquilegia vulgaris L.), Snowdrop windflower (Anemone sylvestris L.), Manchurian monk’s-hood (Aconitum variegatum L.), Turk’s cap lily (Lilium martagon L.), St. Lucie cherry (Cerasus mahaleb (L.) Mill.), Yellow Monkshood (Aconitum anthora L.), Bladdernut (Staphylea pinnata L.) and Edelweiss (Leontopodium alpinum Cass.). The aim of project learning is the interconnection between school theory and practice, and the upbringing towards independence and responsibility. The pupils in the second primary education stage developed these skills by elaborating a card file of plants and a phytogeographical map of the range of the protected species.

Illír bükkös (Vicio oroboidi-Fagetum) erdőtársulás részletes cönológiai vizsgálata

The investigated illyrian beech forest is situated in SW Hungary. Its canopy is dominated by Tilia tomentosa and Fagus sylvatica. Statistics of phytosociological characters, flora elements, life forms and social behaviour types are given. The surveyed stands are near-natural characterised by following specialists: Ruscus hypoglossum, Lathyrus venetus, Aremonia agrimonioides, Staphylea pinnata, Galanthus nivalis, Isopyrum thalictroides, Listera ovata, Anemone nemorosa, Hepatica nobilis

The past, present and future tasks of Hungarian dendrological research

Hungarian dendrological research (research of living woody plants) has more than 200 years old history; the first general work by János Keresztély Grossinger was published in 1797. Further basic works in our time yet are: Forest Botany by Lajos Fekete and Sándor Mágócsy-Dietz (1896); and the chorological work, Distribution of trees and shrubs of sylvicultural importance in the region of Hungarian State by Lajos Fekete and Tibor Blattny (1913). A few dendrologists and many botanists have helped to get better knowledge of Hungarian dendroflora. From the point of view of taxonomy, chorology and habitat - which are interested by field botanists - it can be said that knowledge is fairly heterogeneous. There are sufficient information about most of the rare (protected/endangered) woody plants (an about 50 species) and the important adventives, above all invasive trees and shrubs (an about 10 species). From these two groups beyond there are only few taxa which can be said thoroughly worked up and known (e.g. Castanea sativa, Cornus mas, Fraxinus spp., Quercus spp.). List of the dendrotaxa, hardly known in the above-mentioned point of view is rich in species that are important for forestry or horticulture (e.g. Alnus glutinosa, Acer spp., Betula pendula, Corylusavellana and most of Salix spp.), supplemented with other species (e.g. Clematis vitalba, Colutea arborescens, Lonicera xylosteum, Padus avium, Sambucus nigra, Staphylea pinnata, Viburnum spp.).Followings can be asked from our field botanists: i) look for a specialist in cases of critical dendrotaxa; ii) a circumspect identification is necessary - especially in the case of leaves - by right of great number of samples from the adequate part of shoot; iii) keep in view frequent hybridization (e.g. in the case of Betula, Crataegus, Pyrus, Tilia), and frequent appearance of hybrids (e.g. Betula × rhombifolia, Cerasus × eminens, Salix × rubens); iv) appearance of interim forms are usually typical in the cases of species classified into aggregate species (e.g. Quercus petraea agg., Qu. pubescens agg.); v) take intraspecific taxa according to various ecological demands, area, morphological differences into consideration; vi) culture variations cannot be treat as an equal with the species (e.g. Populus spp., Salix spp.); vii) natural ↔ artificial area have to be separated; viii) lend a helping hand in getting taxonomical knowledge of hardly known dendrotaxa.

Isolation and identification of flower oil components from four Staphylea L. species

AbstractChemical composition of the hydrodistilled volatile fraction from flowers of four Staphylea L. species (Staphylea colchica Stev., Staphylea elegans Zab., Staphylea holocarpa Hemsl., Staphylea pinnata L.) was determined by the GC/MS method. Compounds in fresh and dried flowers (stored for 1 year) were determined. Mostly, structures of different oxygenated aliphatic hydrocarbons in all four species were found. Different aldehydes, ketones, esters of higher fatty acids, and hexadecanoic acid were identified, with dominating content of tricosane, hexadecanoic acid and also of heneicosane, pentacosane, heptacosane, and nonacosane. In S. pinnata L. (fresh flowers) some nonaliphatic hydrocarbons, and in S. holocarpa Hemsl. (fresh flowers) esters of higher fatty acids were found.

Found and lost —Staphyleaceaein Greece

Abstract Raus, Th.: Found and lost — Staphyleaceae in Greece. — Willdenowia 36 (Special Issue): 311–315. — ISSN 0511-9618; © 2006 BGBM Berlin-Dahlem. doi:10.3372/wi.36.36125 (available via http://dx.doi.org/) The angiosperm family Staphyleaceae, represented in the Flora Hellenica area by just one genus and species, Staphylea pinnata, was not known to occur in Greece until recently. Alas, its single known Greek population is definitely destroyed. As a consequence, the status of extinction in Greece has to be attributed to the family, genus and species as long as a reconfirming collection is wanting.

A Rapid Tandem Mass Spectrometric Assay for Determination of Ursolic Acid – Application to Analysis of Ursolic Acid in Four Species of Staphylea L. and Leaves of Staphylea Pinnata L. Gathered During Ontogenesis

Plant extracts of Staphylea L. exhibit a number of biological activities which are presumably attributed to ursolic acid. A rapid and specific tandem mass spectrometric (MS-MS) assay for the quantification of ursolic acid in the leaves of four species of Staphylea L. (Bladdernut) and in the leaves of S. pinnata L. during ontogenesis, was developed and validated. The samples were analyzed by flow injection analysis without chromatographic separation using a transport liquid of methanol/water/formic acid (80:20:0.1 v/v/v) at a flow-rate of 0.2 mL min−1. The run cycle time was ~2-3 min injection-to-injection. Quantification was achieved using multiple reaction monitoring at MRM transition m/z 439 > 203. Calibration curves were linear over the concentration range of 2–20 µg mL−1 with a lower limit of quantification of 2 µg mL−1 (1.8 ± 0.297, RSD: 0.165). Validation data showed that the RSD% values were in the range of 1.8 to 6.8%, whereas the % DEVs ranged from −18 to −2% indicating reasonable and acceptable precision and accuracy, respectively. A recovery percent of 106.8 ± 10.3 of ursolic acid from spiked extracts samples, indicated the specificity and reliability of tandem mass procedure for determination of ursolic acid in the plant extracts. The derived data of sample analysis showed different contents of ursolic acid among various Staphylea species. The highest content of ursolic acid was found in the leaves extract of S. pinnata L. Additionally, the highest amount of ursolic acid accumulated in the leaves of S. pinnata L. was in the August /September period of the year. Smaller amounts of ursolic acid were found in samples collected before and after that time. The results obtained serve as a justification of determining the most appropriate time for collecting plant material as a source of ursolic acid.

Lectotypification of Aizoon hispanicum, Plantago albicans, and Staphylea pinnata, names of three Linnaean species occurring in Calabria (S. Italy)

The typification of the names of three Linnean species which occur in Calabria (S. Italy) is discussed. An illustration in a pre–Linnaean work of Dillenius is designated as the lectotype of Aizoon hispanicum L. (Aizoaceae); a specimen from the Clifford Herbarium (BM) is designated lectotype for Staphylea pinnata L. (Staphyleaceae), and one from the Linnaean Herbarium (LINN) is designated for Plantago albicans L. (Plantaginaceae).

Chromosome numbers of flowering plants from Calabria, S Italy

Peruzzi, L. & Cesca, G.: Chromosome numbers of flowering plants from Calabria, S Italy. — Willdenowia 32: 33–44. 2002. — ISSN 0511-9618.Chromosome numbers of 11 taxa from nine families of Calabrian angiosperms are reported: Anthyllis hermanniae 2n = 14, Carlina acaulis subsp. caulescens 2n = 20, Gentianella crispata 2n = 56, Lupinus graecus 2n = 50, Plantago albicans 2n = 30, P. amplexicaulis subsp. amplexicaulis 2n = 10, Ranunculus flammula subsp. flammula 2n = 32, Saxifraga aizoides 2n = 26, Staphylea pinnata 2n = 26, Tricholaena teneriffae 2n = 36, Veronica scutellata 2n = 18. Most of the reports are the first from Italian populations. Mitotic metaphase microphotographs and haploid idiograms or drawings are presented for all taxa studied. Brief comments are given on karyotype morphology, cytogeography and relationships of selected taxa.

Cytotoxic effects of plant extracts from the families Fabaceae, Oleaceae, Philadelphaceae, Rosaceae and Staphyleaceae.

A human transformed line of HeLa cells was treated with extracts prepared from Gymnocladus dioicus, Holodiscus discolor, Stephanandra tanakae, Ligustrum delavayanum, Ligustrum vulgare and Staphylea pinnata. The study evaluating the influence of the active extracts on HeLa cell division also reveals their acute, delayed and combined effect. A 35% degradation of HeLa cells was found after 72 h treatment with 62.5 microg/mL of the extract isolated from Stephanandra tanakae. A 100% lysis of HeLa cells was observed after 72 h treatment with a 125 microg/mL concentration of the extract prepared from Gymnocladus dioicus. The extracts from Ligustrum devayanum and Ligustrum vulgare were specifically effective only with HeLa cells. On the other hand, the extract prepared from Gymnocladus dioicus was effective on the bacteria and on the HeLa cells.