Differential Stability Research Articles

Interval Type-2 Fuzzy Differential Equations and Stability

This article is devoted to present a framework for studying type-2 fuzzy calculus. Some new concepts pertaining to interval type-2 fuzzy functions (IT2FF) and interval type-2 fuzzy differential equations (IT2FDE) with their stability analysis are introduced. A new definition of type-2 fuzzy derivative called type-2 granular derivative (T2gr-derivative) is defined to overcome the disadvantages of the previous definitions of type-2 fuzzy derivatives. Furthermore, the definitions of elementary arithmetic on the set of interval type-2 fuzzy numbers (IT2FNs) and the set of interval type-2 fuzzy complex numbers are presented. The notion of granular metric on the set of IT2FNs is also defined. Besides, the concepts of continuity, integral, and limit of IT2FFs are introduced. Using the concept of type-2 granular fuzzy Laplace transform and the approximations of T2gr-derivative proposed in this article, the exact and approximate solutions of interval T2FDE are presented. Then, some new concepts such as fuzzy node, fuzzy saddle spiral point, fuzzy star, and so forth lay the groundwork for the analysis of interval T2FDE stability. Several examples are given to illustrate the presented notions.

The transported cations impose differences in the thermostability of the gastric H,K-ATPase. A kinetic analysis

This work analyses the thermostability of a membrane protein, the gastric H,K-ATPase, by means of a detailed kinetic characterization of its inactivation process, which showed to exhibit first-order kinetics. We observed parallel time courses for the decrease of ATPase activity, the decrease of the autophosphorylation capacity and the loss of tertiary structure at 49 °C. Higher temperatures were required to induce a significant change in secondary structure. The correspondence between the kinetics of Trp fluorescence measured at 49 °C and the decrease of the residual activity after heating at that temperature, proves the irreversibility of the inactivation process. Inactivation proceeds at different rates in E1 or E2 conformations. The K+-induced E2 state exhibits a lower inactivation rate; the specific effect is exerted with a K0.5 similar to that found at 25 °C, providing a further inkling that K+ occlusion by the H,K-ATPase is not really favoured. Increasing [H+] from pH 8 to pH 7, which possibly shifts the protein to E1, produces a subtle destabilizing effect on the H,K-ATPase. We performed a prediction of potential intramolecular interactions and found that the differential stability between E1 and E2 may be mainly explained by the higher number of hydrophobic interactions in the α- and β-subunits of E2 conformation.

Rolling Bearing Fault Diagnosis Based on MFDFA-SPS and ELM

Rolling bearings, as important parts on supporting rotating shafts, frequently suffer from fatigue failures. If these rolling bearing failures are not found in time, it will have a huge impact on the whole mechanical system’s operating safety and operating life. To improve the diagnosis of different faults as well as different degrees of faults, a fault diagnosis method based on the multifractal detrended fluctuation analysis (MFDFA) method-singularity power spectrum (SPS) with extreme learning machine (ELM) is proposed. First, MFDFA and SPS analyses are performed on vibration acceleration signals with different faults and different degrees of damage under the same operating conditions, the spectral parameters of stability and quantitative description of differentiation are selected for feature extraction, and then the selected six feature parameters are put into the extreme learning machine for fault classification. The effectiveness of the MFDFA-SPS feature extraction method is demonstrated by analyzing and testing the measured bearing signals. The fault diagnosis accuracy of the bearing fault signals can reach 99.2% based on the MFDFA-SPS with ELM method by using the Case Western Reserve database. The improvements are 6.79% and 18.42% compared to the fault diagnosis methods based on MFDFA with ELM and SPS with ELM. Compared with the methods based on MFDFA-SPS with LSSVM classifier and SVM classifier, the accuracy improvements are 3.54% and 4.25%, respectively. The results show that the method proposed in this paper can achieve the diagnosis of bearing faults and the method based on MFDFA-SPS with ELM is more efficient than the methods based on MFDFA-SPS with LSSVM and SVM classifiers, which is suitable for practical engineering problem-solving.

Adaptive saturated tracking control for solid launch vehicles in ascending based on differential inclusion stabilization

The large-range uncertainties of specific impulse, mass flow per second, aerodynamic coefficients and atmospheric density during rapid turning in solid launch vehicles (SLVs) ascending leads to the deviation of the actual trajectory from the reference one. One of the traditional trajectory tracking methods is to observe the uncertainties by Extended State Observer (ESO) and then modify the control commands. However, ESO cannot accurately estimate the uncertainties when the uncertainty ranges are large, which reduces the guidance accuracy. This paper introduces differential inclusion (DI) and designs a controller to solve the large-range parameter uncertainties problem. When above uncertainties have large ranges, it can be combined with the ascent dynamic equation and described as a DI system in the mathematical form of a set. If the DI system is stabilized, all the subsets are stabilized. Different from the traditional controllers, the parameters of the designed controller are calculated by the uncertain boundaries. Therefore, the controller can solve the problem of large-range parameter uncertainties of in ascending. Firstly, the ascent deviation system is obtained by linearization along the reference trajectory. The trajectory tracking system with engine parameters and aerodynamic uncertainties is described as an ascent DI system with respect to state deviation, which is called DI system. A DI adaptive saturation tracking controller (DIAST) is proposed to stabilize the DI system. Secondly, an improved barrier Lyapunov function (named time-varying tangent-log barrier Lyapunov function) is proposed to constrain the state deviations. Compared with traditional barrier Lyapunov function, it can dynamically adjust the boundary of deviation convergence, which improve the convergence rate and accuracy of altitude, velocity and LTIA deviation. In addition, the correction amplitudes of angle of attack (AOA) and angle of sideslip (AOS) need to be limited in order to guarantee that the overload constraint is not violated during actual flight. In this paper, a fixed time adaptive saturation compensation auxiliary system is designed to shorten the saturation time and accelerate the convergence rate, which eliminates the adverse effects caused by the saturation. Finally, it is proved that the state deviations are ultimately uniformly bounded under the action of DIAST controller. Simulation results show that the DI ascent tracking system is stabilized within the given uncertainty boundary values. The feasible bounds of uncertainty is broadened compared with Integrated Guidance and Control algorithm. Compared with Robust Gain-Scheduling Control method, the robustness to the engine parameters are greatly improved and the control variable is smoother.

Abstract 1578: Interaction of E-cadherin and EGFR in the collective cell migration of colon cancer cells

Abstract Collective cell migration is one of the most important cellular events where a cluster of cells moves together in a coordinated way. Many studies have found this phenomenon as the hallmark of cancer progression and metastasis. In epithelial single cell migration, downregulation of adherens junction (AJ) molecule E-cadherin occurs at the initial step known as the Epithelial to Mesenchymal Transition (EMT). However, in collective cell migration, E-cadherin junction is maintained to provide adhesion between cells. Moreover, it also mediates the cell-cell intercommunication that is critical, for instance, to alter the group polarization through actin cytoskeleton remodeling. Interestingly, Epidermal Growth Factor Receptor (EGFR) was found to colocalize with E-cadherin in the cell-cell contact region, implicating that E-cadherin and EGFR might interact in the cell junction of collective cell migration. To address this, a human colorectal cancer cell line exhibiting epithelial phenotype HT-29 was used. The cell density and calcium presence were modified to infer the effect of differential expression and stability of E-cadherin on the cellular signaling of proliferation and migration. The results showed that stable and enhanced expression of E-cadherin downregulates EGFR expression and reduces its activity. Moreover, the Src phosphorylation increased when the cell-cell contact was strengthened via calcium addition and decreased when the cell-cell contact was diminished via calcium chelation. Overall, this indicates that E-cadherin expression at the cell-cell junction and contact stability determine the EGFR expression, activity, and Src phosphorylation. With the goal of deciphering the relationship of E-cadherin and EGFR in cell contact, proliferation, and migration, we intend to finally implement the knowledge as a strategy or approach in promoting alternative methods to inhibit metastasis. Citation Format: Viriya Adhiguna Winarso, Yi-Wen Lu, Yu-Cheng Weng, Xiang-Ling Hou, Hui Min Koo, Yu-n Li, Wei-Ting Chao. Interaction of E-cadherin and EGFR in the collective cell migration of colon cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 1578.

Platelet-Rich Plasma as an Alternative to Xenogeneic Sera in Cell-Based Therapies: A Need for Standardization.

There has been an explosion in scientific interest in using human-platelet-rich plasma (PRP) as a substitute of xenogeneic sera in cell-based therapies. However, there is a need to create standardization in this field. This systematic review is based on literature searches in PubMed and Web of Science databases until June 2021. Forty-one studies completed the selection criteria. The composition of PRP was completely reported in less than 30% of the studies. PRP has been used as PRP-derived supernatant or non-activated PRP. Two ranges could be identified for platelet concentration, the first between 0.14 × 106 and 0.80 × 106 platelets/µL and the second between 1.086 × 106 and 10 × 106 platelets/µL. Several studies have pooled PRP with a pool size varying from four to nine donors. The optimal dose for the PRP or PRP supernatant is 10%. PRP or PRP-derived supernatants a have positive effect on MSC colony number and size, cell proliferation, cell differentiation and genetic stability. The use of leukocyte-depleted PRP has been demonstrated to be a feasible alternative to xenogeneic sera. However, there is a need to improve the description of the PRP preparation methodology as well as its composition. Several items are identified and reported to create guidelines for future research.

From retinal organoids to “retinal organ”

<p indent="0mm">Retinal degenerative disease is a common ophthalmological disease that causes severe blindness, and difficult to treat in clinics at present. Because retinal tissue is not easy to obtain and cannot be cultured, it is difficult to study the mechanism and treatment of retinal-related diseases. Due to the significant species differences between the retina of animals and humans, the results of many animal experiments in the past were often difficult to be utilized to solve clinical problems. The emergence of retinal organoids has become a new hope for solving these problems. It enables retinal-related diseases to be reproduced in cell culture dishes for research on disease mechanisms and drug screening. More importantly, it can be used for cell transplantation to replace degenerated retinal cells, which is expected to restore sight to the blind. However, we must also recognize that there are still some bottlenecks that need to be solved urgently in retinal organoid simulation diseases, such as the low stability of differentiation, lack of blood vessels and neuroimmune cells. Although the application of micro-millifluidic bioreactor has improved the differentiation of retinal organs, current technology can only achieve a level of “organoids” and has not yet reached the standard of “retinal organ”. Therefore, the purpose of this review is to sort out the applications and existing problems of retinal organoids, and think about how to further promote the development of retinal organoids that can be used for regenerative medicine, in order to better facilitate the treatment and research of retinal diseases.

Fractional second linear multistep methods: the explicit forms for solving fractional differential equations and stability analysis

In this paper, the explicit forms of the fractional second linear multistep methods (FSLMMs) are introduced for solving fractional differential equations (FDEs) of the fractional-order in . These explicit FSLMMs are constructed based on fractional backward difference formulas 1, 2, and 3 (FBDF1, FBDF2, and FBDF3) with the first, second, third, and fourth orders of convergence. Also, the monotonicity of these FBDFs is considered when the order of fractional derivatives lies into . The order of consistency, linear stability, and the order of convergence of these explicit methods are analysed. Moreover, the stability regions of the proposed methods are completely studied in the stability topic. Finally, four experimental examples are presented to confirm the proposed theories.

Neuronal Cells Display Distinct Stability Controls of Alternative Polyadenylation mRNA Isoforms, Long Non-Coding RNAs, and Mitochondrial RNAs.

RNA stability plays an important role in gene expression. Here, using 3′ end sequencing of newly made and pre-existing poly(A)+ RNAs, we compare transcript stability in multiple human cell lines, including HEK293T, HepG2, and SH-SY5Y. We show that while mRNA stability is generally conserved across the cell lines, specific transcripts having a high GC content and possibly more stable secondary RNA structures are relatively more stable in SH-SY5Y cells compared to the other 2 cell lines. These features also differentiate stability levels of alternative polyadenylation (APA) 3′UTR isoforms in a cell type-specific manner. Using differentiation of a neural stem cell line as a model, we show that mRNA stability difference could contribute to gene expression changes in neurogenesis and confirm the neuronal identity of SH-SY5Y cells at both gene expression and APA levels. In addition, compared to transcripts using 3′-most exon cleavage/polyadenylation sites (PASs), those using intronic PASs are generally less stable, especially when the PAS-containing intron is large and has a strong 5′ splice site, suggesting that intronic polyadenylation mostly plays a negative role in gene expression. Interestingly, the differential mRNA stability among APA isoforms appears to buffer PAS choice in these cell lines. Moreover, we found that several other poly(A)+ RNA species, including promoter-associated long noncoding RNAs and transcripts encoded by the mitochondrial genome, are more stable in SH-SY5Y cells than the other 2 cell lines, further highlighting distinct RNA metabolism in neuronal cells. Together, our results indicate that distinct RNA stability control in neuronal cells may contribute to the gene expression and APA programs that define their cell identity.

Expression of the Cyanobacterial FoF1 ATP Synthase Regulator AtpΘ Depends on Small DNA-Binding Proteins and Differential mRNA Stability.

ABSTRACTFoF1 ATP synthases produce ATP, the universal biological energy source. ATP synthase complexes on cyanobacterial thylakoid membranes use proton gradients generated either by photosynthesis or respiration. AtpΘ is an ATP synthase regulator in cyanobacteria which is encoded by the gene atpT. AtpΘ prevents the hydrolysis of ATP (reverse reaction) that otherwise would occur under unfavorable conditions. In the cyanobacterium Synechocystis sp. PCC 6803, AtpΘ is expressed maximum in darkness but at very low levels under optimum phototrophic growth conditions or in the presence of glucose. DNA coimmunoprecipitation experiments followed by mass spectrometry identified the binding of the two transcriptional regulators cyAbrB1 and cyAbrB2 to the promoter and the histone-like protein HU to the 5’UTR of atpT. Analyses of nucleotide substitutions in the promoter and GFP reporter assays identified a functionally relevant sequence motif resembling the HLR1 element bound by the RpaB transcription factor. Electrophoretic mobility shift assays confirmed interaction of cyAbrB1, cyAbrB2, and RpaB with the promoter DNA. However, overall the effect of transcriptional regulation was comparatively low. In contrast, atpT transcript stabilities differed dramatically, half-lives were 1.6 min in the light, 33 min in the dark and substantial changes were observed if glucose or DCMU were added. These findings show that transcriptional control of atpT involves nucleoid-associated DNA-binding proteins, positive regulation through RpaB, while the major effect on the condition-dependent regulation of atpT expression is mediated by controlling mRNA stability, which is related to the cellular redox and energy status.IMPORTANCE FoF1 ATP synthases produce ATP, the universal biological energy source. Under unfavorable conditions, ATP synthases can operate in a futile reverse reaction, pumping protons while ATP is used up. Cyanobacteria perform plant-like photosynthesis, but they cannot use the same mechanism as plant chloroplasts to inhibit ATP synthases during the night because respiratory and photosynthetic complexes are both located in the same membrane system. AtpΘ is a small protein encoded by the gene atpT in cyanobacteria that can prevent the ATP synthase reverse reaction (ATPase activity). Here we found that three transcription factors contribute to the regulation of atpT expression. However, the control of mRNA stability was identified as the major regulatory process governing atpT expression. Thus, it is the interplay between transcriptional and posttranscriptional regulation that position the AtpΘ-based regulatory mechanism within the context of the cellular redox and energy balance.

Macrophage inflammation resolution requires CPEB4-directed offsetting of mRNA degradation.

Chronic inflammation is a major cause of disease. Inflammation resolution is in part directed by the differential stability of mRNAs encoding pro-inflammatory and anti-inflammatory factors. In particular, tristetraprolin (TTP)-directed mRNA deadenylation destabilizes AU-rich element (ARE)-containing mRNAs. However, this mechanism alone cannot explain the variety of mRNA expression kinetics that are required to uncouple degradation of pro-inflammatory mRNAs from the sustained expression of anti-inflammatory mRNAs. Here, we show that the RNA-binding protein CPEB4 acts in an opposing manner to TTP in macrophages: it helps to stabilize anti-inflammatory transcripts harboring cytoplasmic polyadenylation elements (CPEs) and AREs in their 3'-UTRs, and it is required for the resolution of the lipopolysaccharide (LPS)-triggered inflammatory response. Coordination of CPEB4 and TTP activities is sequentially regulated through MAPK signaling. Accordingly, CPEB4 depletion in macrophages impairs inflammation resolution in an LPS-induced sepsis model. We propose that the counterbalancing actions of CPEB4 and TTP, as well as the distribution of CPEs and AREs in their target mRNAs, define transcript-specific decay patterns required for inflammation resolution. Thus, these two opposing mechanisms provide a fine-tuning control of inflammatory transcript destabilization while maintaining the expression of the negative feedback loops required for efficient inflammation resolution; disruption of this balance can lead to disease.

Chromosomal aberration and genetic differentiation of Oreochromis niloticus affected by heavy metals from an iron ore mine area

ABSTRACT The concentrations of As, Cd, Cr, Pb and Fe in water, sediment and Oreochromis niloticus tissues and the chromosomal aberrations, genetic differentiation and genomic template stability (GTS) of O. niloticus in 2 reservoirs near iron ore mine and one reference reservoir were investigated. Genetic differentiation and %GTS were evaluated by inter simple sequence repeat analysis. The As, Cd and Cr concentrations in fish from 2 mine reservoirs exceeded the standards. Seven types of chromosomal aberrations were found in fish from the iron ore mine: single chromatid gap, iso chromatid gap, centromere gap, dicentric chromosome, fragmentation, deletion, and ring chromosome. The average percentages of aberrant chromosomes in the cells of O. niloticus from mine reservoirs 1, 2 and the reference reservoir were 23.6, 16.8 and 2.2, respectively. Genetic differentiation and subsequent dendrogram analysis separated O. niloticus into 3 clusters, corresponding to the 2 mine reservoirs and the reference reservoir. The %GTS ranges of O. niloticus were 68.75–83.75, 71.88–85.19 and 83.58–97.01, respectively. Metals accumulations in O. niloticus could induce the occurrence of chromosomal aberrations and genetic differentiation.

Determinants of heritable gene silencing for KRAB-dCas9+DNMT3 and Ezh2-dCas9+DNMT3 hit-and-run epigenome editing.

Precision epigenome editing has gained significant attention as a method to modulate gene expression without altering genetic information. However, a major limiting factor has been that the gene expression changes are often transient, unlike the life-long epigenetic changes that occur frequently in nature. Here, we systematically interrogate the ability of CRISPR/dCas9-based epigenome editors (Epi-dCas9) to engineer persistent epigenetic silencing. We elucidated cis regulatory features that contribute to the differential stability of epigenetic reprogramming, such as the active transcription histone marks H3K36me3 and H3K27ac strongly correlating with resistance to short-term repression and resistance to long-term silencing, respectively. H3K27ac inversely correlates with increased DNA methylation. Interestingly, the dependance on H3K27ac was only observed when a combination of KRAB-dCas9 and targetable DNA methyltransferases (DNMT3A-dCas9 + DNMT3L) was used, but not when KRAB was replaced with the targetable H3K27 histone methyltransferase Ezh2. In addition, programmable Ezh2/DNMT3A + L treatment demonstrated enhanced engineering of localized DNA methylation and was not sensitive to a divergent chromatin state. Our results highlight the importance of local chromatin features for heritability of programmable silencing and the differential response to KRAB- and Ezh2-based epigenetic editing platforms. The information gained in this study provides fundamental insights into understanding contextual cues to more predictably engineer persistent silencing.

Change and Continuity in Attitudes Toward Homosexuality Across the Lifespan

ABSTRACT This study examines differential stability in attitudes toward homosexuality using panel data representative of the American adult population. While attitudes toward homosexuality have shifted considerably on the aggregate-level over the past few decades, this study shows that such attitudes are remarkably stable on the individual-level. Employing conditional change models, this study also provides a test of the aging-stability hypothesis with regard to attitudes toward homosexuality. That hypothesis is confirmed, as attitude stability is found to gradually increase with age. However, no other socio-demographic variables are found to have a consistent relationship with stability. The finding of an age-graded increase in stability suggests that attitudes toward homosexuality are formed predominantly early in life and that susceptibility to attitude change declines across the adult lifespan. This finding also supports a generational replacement explanation of recent changes in American public opinion on homosexuality as aging-stability translates into cohort effects on the aggregate-level.

Linking post-translational modifications and protein turnover by site-resolved protein turnover profiling

Proteome-wide measurements of protein turnover have largely ignored the impact of post-translational modifications (PTMs). To address this gap, we employ stable isotope labeling and mass spectrometry to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated, and ubiquitinated peptides for >9,000 native proteins. This site-resolved protein turnover (SPOT) profiling discloses global and site-specific differences in turnover associated with the presence or absence of PTMs. While causal relationships may not always be immediately apparent, we speculate that PTMs with diverging turnover may distinguish states of differential protein stability, structure, localization, enzymatic activity, or protein-protein interactions. We show examples of how the turnover data may give insights into unknown functions of PTMs and provide a freely accessible online tool that allows interrogation and visualisation of all turnover data. The SPOT methodology is applicable to many cell types and modifications, offering the potential to prioritize PTMs for future functional investigations.

Stability Control Method of Hybrid Impulsive System Based on M-Matrix

Aiming at the problems of poor control effect and poor stability of the mixed pulse system with the traditional method, this paper introduces the M-matrix to establish the pulse delay differential indefinite formula and realize stability control of the mixed pulse system. The synchronization problem of mixed-pulse systems in complex networks is analyzed using M matrix. The local coupling strength of the impulsive system is controlled according to the adaptive method. A class of Multi-Lyapunov functions is constructed for stability control of hybrid impulsive systems. The proposed method is proved to have better control effect through experiments.

Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture.

Long-term stable cell culture is a critical tool to better understand cell function. Most adherent cell culture models require a polymer substrate coating of poly-lysine or poly-ornithine for the cells to adhere and survive. However, polypeptide-based substrates are degraded by proteolysis and it remains a challenge to maintain healthy cell cultures for extended periods of time. Here, we report the development of an enhanced cell culture substrate based on a coating of dendritic polyglycerol amine (dPGA), a non-protein macromolecular biomimetic of poly-lysine, to promote the adhesion and survival of neurons in cell culture. We show that this new polymer coating provides enhanced survival, differentiation and long-term stability for cultures of primary neurons or neurons derived from human induced pluripotent stem cells (hiPSCs). Atomic force microscopy analysis provides evidence that greater nanoscale roughness contributes to the enhanced capacity of dPGA-coated surfaces to support cells in culture. We conclude that dPGA is a cytocompatible, functionally superior, easy to use, low cost and highly stable alternative to poly-cationic polymer cell culture substrate coatings such as poly-lysine and poly-ornithine. Summary statement Here, we describe a novel dendritic polyglycerol amine-based substrate coating, demonstrating superior performance compared to current polymer coatings for long-term culture of primary neurons and neurons derived from induced pluripotent stem cells.

Dynamical Behavior of a Predator‐Prey System Incorporating a Prey Refuge with Impulse Effect

A predator‐prey model with Holling II functional response incorporating a prey refuge with impulse effect is considered in this paper. With the help of the Floquet theory of impulsive differential equations, local stability and global attractivity of the boundary periodic solution of the system are derived, and then sufficient conditions for global asymptotic stability of the boundary periodic solution are obtained. Next, the permanence of the system is proved by constructing a Lyapunov function. Further, by applying the bifurcation theory of impulsive differential equations, conditions under which the system has a positive periodic solution are obtained. Finally, numerical simulations are presented to illustrate the analytical results.

The catalytic activity and adsorption in faujasite and ZSM-5 zeolites: the role of differential stabilization and charge delocalization.

Adsorption and chemical reactions occurring on industrially important ZSM-5 and faujasite zeolite catalysts are investigated with the quantum-mechanical fragment molecular orbital method combined with periodic boundary conditions. Suitable fragmentation patterns are devised and tested providing important case studies of computing real materials with fragmentation methods. A good accuracy is demonstrated in comparison to full calculations, and a good agreement with the available experimental data is obtained. The full production cycle of p-xylene on faujasite zeolite is mapped. The catalytic role of the zeolite in the dehydration reaction, analyzed with the partition analysis, is attributed to the delocalization of the negative charge over the zeolite. On the other hand, an increase of the barrier in the Diels-Alder reaction by the zeolite is attributed to the preferential stabilization of the reactants over the transition state as demonstrated by the guest-zeolite interaction energy.

Stabilita a změna osobních hodnot u lidí s různými trajektoriemi životní dráhy: longitudinální studie

Background. Previous research on the stability of personal values in the context of life transitions has usually focused on the presence of a single transition. However, life transitions in everyday life occur simultaneously with other life transitions. The aim of this longitudinal study was therefore to identify different trajectories of life transitions in young adults and to compare the stability of their personal values. Method. In the first wave of research, participants were 18-33 years old; in the second wave, they were 29-43 years old (N = 632; 392 women). In both waves, they completed Schwartzʼs Portrait Value Questionnaires (PVQ); in the second wave, they completed the Life History Calendar focusing on the presence of entry into life transitions. Results. Latent class analysis revealed two trajectories: Experienced transitions (people who experienced all observed transitions) and Partially experienced transitions (people who experienced only entry into regular employment and part of them entered cohabitation). Differential stability of personal values occurred in both trajectories. The differences in the stability of values found between the individual trajectories were insignificant, except for personal values universalism and tradition. Although before entry into life transitions, personal values were not significant predictors of belonging to a particular trajectory, after their experiencing people with the Experienced transitions trajectory more often reached lower levels of values associated with openness to change.