Spring Samples Research Articles

Does Microbial and Faunal Pattern Correspond to Dynamics in Hydrogeology and Hydrochemistry? Comparative Study of Two Isolated Groundwater Ecosystems in Münsterland, Germany

The objective of this study was to assess the temporal and spatial variability of aquatic invertebrates and microbial parameters (biomass and activity) with environmental data in springs, and to determine the impact of key parameters on the ecological situation of a groundwater system. Eight springs in the two study areas of Baumberge and Schöppinger Berg (W-NW of Münster, North Rhine Westphalia, Germany) were sampled at three sampling campaigns between 2018 and 2019. Physicochemical parameters of the spring samples and abundances of aquatic invertebrates were determined at each sampling event. Samples for hydro(geo)chemical and microbial analyses were collected during each sampling campaign in the springs. Spearman correlation and principal component analysis were used to identify the key parameters. The abundance of aquatic invertebrates and microbial activity were significantly positively correlated with groundwater table fluctuation. The abundance of stygobite individuals was significantly positively correlated with the Groundwater-Fauna-Index and phosphate in Baumberge, and negatively correlated with chloride in Schöppinger Berg. Most notably, the stable isotopes of water and microbial activity were significantly inversely correlated. The hydro(geo)chemical results showed no significant spatial differences in groundwater in both groundwater systems. Stable isotopes of water indicate a meteoric origin, with an effect of evaporation for two months, even though the downward percolation and groundwater recharge rates are high. The nitrate concentration was higher than 50 mg/L only in SB due to the agricultural activities. Nitrate input into groundwater comes from two sources in Baumberge, while it comes from one source in Schöppinger Berg. There was no evidence of denitrification in both areas. Secondary gypsum is assumed to be the source of sulfate in groundwater in Schöppinger Berg, but anaerobic oxidation of pyrite in the deeper part of the groundwater system as a source of sulfate cannot be excluded.

Methanogens-Driven Arsenic Methylation Preceding Formation of Methylated Thioarsenates in Sulfide-Rich Hot Springs

Hot springs represent a major source of arsenic release into the environment. Speciation is typically reported to be dominated by arsenite, arsenate, and inorganic thiolated arsenates. Much less is known about the relevance and formation of methylated thioarsenates, a group with species of high mobility and toxicity. In hot spring samples taken from the Tengchong volcanic region in China, methylated thioarsenates contributed up to 13% to total arsenic. Enrichment cultures were obtained from the corresponding sediment samples and incubated to assess their capability to convert arsenite into methylated thioarsenates over time and in the presence of different microbial inhibitors. In contrast to observations in other environmental systems (e.g., paddy soils), there was no solid evidence, supporting that the sulfate-reducing bacteria contributed to the arsenic methylation. Methanosarcina, the sole genus of methanogens detected in the enrichment cultures, as well as Methanosarcina thermophila TM-1, a pure strain within the genus, did methylate arsenic. We propose that methylated thioarsenates in a typical sulfide-rich hot spring environment like Tengchong form via a combination of biotic arsenic methylation driven by thermophilic methanogens and arsenic thiolation with either geogenic sulfide or sulfide produced by sulfate-reducing bacteria.

The presence of antibiotics and multidrug-resistant Staphylococcus aureus reservoir in a low-order stream spring in central Brazil.

The disposal of industrial effluents strongly influences low-order streams, which makes them fragile ecosystems that can be impacted by contamination. In central Brazil, the Extrema River spring targets the dumping of pharmaceutical products from the surrounding industries. So, this work aimed to investigate the presence of antibiotics in Extrema River spring samples and the isolation of Staphylococcus aureus, a potential multidrug-resistant bacteria, verifying the antimicrobial resistance profile of these isolates. Three campaigns were carried out in different locals (P1-P3) between October and December 2021, in the dry and rainy seasons. The high-performance liquid chromatography-tandem mass spectrometry (LCMS) approach indicated the presence of sulfamethoxazole (≥ 1 ng/L), metronidazole (< 0.5 ng/L), and chloramphenicol (< 5 ng/L) in the water samples in November (rainy season). S. aureus was isolated in P1 (n = 128), P2 (n = 168), and P3 (n = 36), with greater resistance to trimethoprim-sulfamethoxazole (90%), clindamycin (70%), and gentamicin (60%). The presence of antibiotics in the Extrema River spring may cause S. aureus antibiotic resistance development. The presence of antibiotics and the high percentage of isolated multidrug-resistant S. aureus in the Extrema River spring cause concern and indicate the clandestine dumping of effluents from nearby pharmaceutical industries. Since preserving the springs of low-order streams is important for the environment and public health, we encourage monitoring the wastewater from Extrema River's nearby pharmaceutical industries and preserving the spring of this river.

Dynamic Finite Element Model Based on Timoshenko Beam Theory for Simulating High-Speed Nonlinear Helical Springs

Helical springs with nonlinear geometric parameters nowadays have shown great advantages over classical linear springs, especially due to their superior performance in diminishing dynamic responses in high-speed situations. However, existing studies are mostly available for springs with linear properties, and the sole FE spring models using solid elements occupy significant computational resources. This study presents an FE spring model based on Timoshenko beam theory, which allows for high-speed dynamic simulations of nonlinear springs using a beehive valve spring sample. The dynamic results are also compared with the results of the FE model using solid elements and the results of the engine head test and indicate that the proposed FE model can accurately predict dynamic spring forces and the phenomenon of coil clash when simulating the beehive spring at engine speeds of both 5600 and 8000 RPM. The results also indicate that rapid coil impact brings significant spike forces. It should also be noted that the FE spring model using beam elements displays sufficient accuracy in predicting the dynamic responses of nonlinear springs while occupying much fewer computational resources than the FE model using solid elements.

Origin of lithium-rich salt lakes on the western Kunlun Mountains of the Tibetan Plateau: Evidence from hydrogeochemistry and lithium isotopes

Lithium-rich salt lakes have attracted much attention as important lithium resources. The source and enrichment mechanisms of lithium in salt lakes are fundamental to uncovering the genesis of brine-type lithium deposits. However, the origin of lithium in salt lakes has been controversial, especially for lithium-rich brines associated with hard rock lithium ore has not been concerned by scholars. In this study, for the first time, we investigated the lithium isotope and hydrochemical composition characteristics of a lithium-rich salt lake (Kushui Lake) and a series of surrounding water samples in the lithium pegmatites district of West Kunlun, Xinjiang, China. Hydrochemical composition shows that different samples in the basin are rich in Li, Na, and K and poor in Mg and Ca. The water chemistry of the Kushui River, the largest river within the catchment, changes from carbonate type to sulfate subtype through evaporation. The precipitate of sulfate and chloride minerals is also gradually increasing. In addition, Li, B, Sr, Rb, Cs, U, Ti, V, Mn, Cu, Zn, Cr, Co, Ni, and REE are abnormally enriched in the rivers and lakes of the basin, especially in the spring water, which is several times higher than in the rivers. Likewise, dissolved Li and δ7Li showed ranges of 0.01–287.86 mg/L and 3.28–16.44 ‰, respectively. These data were used to determine the origin of lithium and its enrichment process in salt lakes. The results show that the source of Li in Kushui Lake is closely related to the surrounding felsic volcanic rocks, pegmatite-type lithium ore and deep fluids, whereas the water–rock interaction in the deep crust and the chemical weathering in the supergene environment play an influential role during the formation of Li-rich fluids. In addition, the spring samples in the northwestern of Kushui Lake have inherited the lithium isotopic signature of lithium-bearing source rocks from the water–rock reaction process in the deep crust, allowing us further to isolate the source of Li in the target waters. With the movement of water, lithium exhibits a trend of continuous enrichment under the influence of evaporation and mixing. At different stages of evaporation, the Li/Na ratio in water in this catchment shows a trend of increasing, decreasing, and increasing due to the adsorption of 6Li by secondary minerals and the crystallization of Na. It suggests that strong evaporation under arid conditions is the main mechanism responsible for the rapid enrichment of Li in salt lakes. Moreover, the positive relationship between Li content and the δ7Li value of rivers along the discharge path indicated that the adsorption of 6Li by secondary minerals still existed even at a minor watershed scale, resulting in the fractionation of lithium isotopes in river waters. This study may contribute to a deeper understanding of lithium sources and their enrichment processes in Li-rich salt lakes. In addition, it is also beneficial to search for new lithium resources based on the source relationships among the various lithium reservoirs.

Speleomycology of Air in Stopića Cave (Serbia).

Fungi can colonize organic matter present in subterranean sites and have a significant role as dwellers in different microniches of cave habitats. In order to analyze the content of airborne fungal propagules in different parts of "Stopića Cave," a touristic site in Serbia, air sampling was carried out in three seasons during 2020, prior to and during the onset of COVID-19 pandemic. Culturable mycobiota was identified using both microscopic techniques and ITS region/BenA gene barcoding, while multivariate analyses were employed to establish the link between fungal taxa and different environmental factors. The maximal measured fungal propagule concentrations were recorded during spring sampling which were based on fungal propagule concentration categories; the cave environment matches the category V. A total of 29 fungal isolates were identified, while Aspergillus, Cladosporium, Fusarium, Lecanicillium, Mucor, and Penicillium were the most diverse genera. According to the trophic mode, most of the isolated fungal species were pathotrophs (75.86%), but when regarding ecological guilds, the most dominant were undefined saprobes and animal pathogens (41.38% for each). Show caves are especially vulnerable to human impacts, and the fungal propagules' concentration within the caves could be good indices for the level of ecological disturbance.

High prevalence of hepatitis A and E viruses in environmental and clinical samples from West Argentina.

Environmental surveillance of water sources is important to monitoring viral hepatitis transmission in clinical settings. This study investigated the circulation of hepatitis A (HAV) and E (HEV) viruses in sewage and clinical samples from Argentina. Between 2016 and 2017, 80 raw sewage samples and 86 clinical samples (stool and serum) from suspected cases of hepatitis A and hepatitis E were obtained. HAV and HEV were tested by both real-time and nested PCR. Positive samples were sequenced for genotype determination and phylogenetic analysis. Overall, HAV was recovered in 39% of sewage samples and 61.1% of clinical samples. HEV was detected in 22.5% of sewage samples and 15.9% of clinical samples. HAV was found more frequently in sewage during the winter and in clinical samples in spring; HEV was more prevalent in sewage during summer and in clinical samples in autumn. All HAV isolates belonged to genotype IA and HEV isolates belonged to genotype 3, the most prevalent genotypes in South America. High prevalence of HAV and HEV in environmental and clinical samples in Mendoza, Argentina was observed. These findings reinforce the importance of environmental surveillance and implementation of health strategies to control the spread of HAV and HEV in developing countries.

Spatio-temporal distribution of microplastics in water and sediment samples of the Plankenburg river, Western Cape, South Africa

Microplastic (MPs) pollution has become a subject of environmental concern due to its wide ubiquity in the environment. Microplastics are possible sources of other hazardous chemicals to aquatic organisms due to their composition and sorption properties. In this study, MPs occurrence in water and sediment samples of the Plankenburg River, Western Cape, South Africa was investigated. The physicochemical characterization of the river water was done onsite. 30 L water samples were collected and filtered in 10 L triplicates through a 250 μm mesh onsite using a metal bucket. An additional 12 L sample was collected and filtered in 4 L triplicates through 20 μm mesh in the laboratory. The extraction of MPs from water in the laboratory was by density separation. Sediment samples were also collected at the selected sites, oven-dried and microplastics in the laboratory. Sampling was conducted over four seasons – spring, summer, autumn, and winter. Microplastics were classified by visual observation and Fourier Transform Infrared Spectroscopy (FTIR-ATR). The seasonal distribution of MPs in the surface water samples varied across all sites. However, spring samples had the highest MPs occurrence (5.13 ± 6.62 MP/L) and the least, in autumn (1.52 ± 2.54 MP/L). The MPs in sediment samples were observed in spring (1587.50 ± 599.32 MP/kg). Fibres were the most dominant microplastic particle type (shape), with a size range of 500–1000 μm at the different sites. The infrared spectroscopic analysis confirmed the dominant polymer type to be polyethylene. This study provides an understanding of the microplastic occurrence in the Plankenburg River system and gives a baseline for future monitoring and assessment of water and sediment in the South African freshwater systems.

Antibiotic resistome in groundwater and its association with mountain springs and river

The distribution of antibiotic resistance genes (ARGs) in water sources potentially threatens drinking water safety. However, the sources of antibiotic resistome in groundwater are still under-investigated. Here, we evaluated the profiles of antibiotic resistome in peri-urban groundwater and its associated water sources (river and mountain spring) to characterize the antibiotic resistome from natural water sources on groundwater resistome. A total of 261 antibiotic resistome were detected in groundwater, mountain spring, and river samples. The relative abundances of ARGs and mobile genetic elements (MGEs) were significantly higher in the river samples than in spring water and groundwater samples. The resistome profiles were similar between groundwater and spring water but differed from the river samples. According to source tracking results, the groundwater resistome was likely to be derived from springs (28.0%−50.0%) and rivers (28.6%−48.6%), which share the same trend for the source tracking of bacterial communities. Bacterial α-diversity, bacterial β-diversity, and MGEs directly or indirectly affected the ARGs in groundwater samples. Although the abundance of groundwater resistome was not elevated by river and spring water, groundwater resistomes were diverse and may be derived from both river and spring water. We highlight the importance of groundwater resistome and its association with potential water sources, providing a better understanding and basis for the effective control of the ARG proliferation and dissemination in groundwater from exogenous water bodies in the future.

Hydrochemical and isotopic composition of periglacial watersheds in Northern Patagonian Andes

Mountain periglacial environments are important sites of water storage and distribution to downstream areas. The present study was conducted in the “La Hoya” subwatershed, in the Northern Patagonian Andes. La Hoya is the main water recharge area of the “Los Bandidos” watershed, from which fresh water is used for human consumption. The aim of this work was to study the hydrogeochemical and isotopic composition of this watershed and evaluate the hydrological and ionic contributions from periglacial landforms, accounting for annual water flow hydrodynamics and climatic trends. Meteorological data, spring and stream flows, electrical conductivity, isotopic content, and major ion concentrations of snow, spring, and stream samples were analyzed from December 2020 to November 2021. Snow melting is the main water contribution to the mountain watershed. Part of the snowmelt drains superficially during the melting season and part of it infiltrates into periglacial landforms, where it is provisionally stored as groundwater. Chemical weathering during groundwater-rock interaction is the main process that regulates water chemical composition, dominating the dissolution of CO2(g) and carbonates, which controls the waters to be of the Ca–HCO3 to Ca–Mg–HCO3 facies type of groundwater. The climate tendency for the last sixty years in this area reflects a decrease in precipitation and an increase in temperatures. This climate trend could affect periglacial environments, modifying the chemical and physical processes that take place in them, which could affect the chemical water quality and the hydrological behavior of the watershed. The chemical and hydrological behavior in watersheds for human consumption should be specially monitored for better use of this resource.

Experimental Analysis of Transverse Stiffness Distribution of Helical Compression Springs

AbstractThis paper presents the results of an experimental analysis of the distribution of transverse stiffness of cylindrical compression helical springs with selected values of geometric parameters. The influence of the number of active coils and the design of the end coils on the transverse stiffness distribution was investigated. Experimental tests were carried out for 18 sets of spring samples that differed in the number of active coils, end-coil design and spring index, and three measurements were taken per sample, at two values of static axial deflection. The transverse stiffness in the radial directions were tested at every 30° angle. A total of 1,296 measurements were taken, from which the transverse stiffness distributions were determined. It was shown that depending on the direction of deflection, the differences between the highest and lowest value of transverse stiffness of a given spring can exceed 25%. The experimental results were compared with the results of the formulas for transverse stiffness available in the literature. It was shown that in the case of springs with a small number of active coils, discrepancies between the average transverse stiffness of a given spring and the transverse stiffness calculated based on literature relations can reach several tens of percent. Analysis of the results of the tests carried out allowed conclusions to be drawn, making it possible to estimate the suitability of a given computational model for determining the transverse stiffness of a spring with given geometrical parameters.

Quantifying fish range shifts across poorly defined management boundaries.

Management regimes of marine resources that rely on spatial boundaries might be poorly adapted to climate change shifts in species distributions. This is of specific concern for the management of fish stocks that cross management jurisdictions, known as shared stocks. Transitioning to dynamic rules in spatial management has been suggested as a solution for mismatches between species distributions and the spatial boundaries. However, in many cases spatial boundaries are not clearly drawn, hampering such transitions. Here, we use black sea bass (Centropristis striata), summer flounder (Paralichthys dentatus) and scup (Stenotomus chrysops) as case studies to explore different approaches to designing spatial regulatory units to facilitate the adaptation of fisheries management to shifting distributions of shared stocks. First, we determine the yearly distribution of each stock within the United States Exclusive Economic Zone from 1951 to 2019 during Fall and Spring sampling seasons. Second, we explore two approaches for drawing regulatory units based on state waters and historical landings. Finally, we estimate each state's proportion of the stock's distribution and compare historical and recent values. We show that the distribution of all three stocks has changed relative to the years used to determine the current quota allocation across states, with an overall gain for central-northern states at the expense of the southernmost states. In terms of the distribution of allocation, we find that, while seasonal differences exist, the biggest differences in the proportion of the stock spatial distribution attributed to each state come from the method for designing regulatory units. Here, we show that the method used to define allocation units can have meaningful impacts on resulting adaptive policy. As climate change-driven conflicts in fishing resource allocation are expected to increase and deepen around the world, we provide a replicable approach to make an informed and transparent choice to support data-driven decision-making.

Optimization of amylase production using response surface methodology from newly isolated thermophilic bacteria

Present study was aimed at screening and characterizing thermostable amylase-producing bacteria from water and sediment samples of unexplored hot spring of Tatta Pani Kotli Azad Kashmir. Four thermophilic isolates were characterized on morphological, biochemical, physiological basis and were authenticated by molecular analysis. By 16S rDNA sequencing, isolates were identified as Anoxybacillus mongoliensis (MBT001), Anoxybacillus flavithermus (MBT002), Bacillus (MBT004). Among all identified strains, MBT003 showed maximum homology with both Anoxybacillus mongoliensis and Anoxybacillus flavithermus. Amylase activity was analyzed qualitatively in starch agar and quantitatively by DNS method. The optimal enzyme production was observed and authenticated by Response Surface Methodology at 7 pH, 70 °C, 1.25% substrate concentration, 300 μL of inocula volume after 48 h of incubation. Optimum amylase activity (4.4 U/mL) and stability (3.3 U/mL) was observed with 1.5% soluble starch at 70 °C. Maximum activity (3.7 U/mL) and stability (1.5 U/mL) was found at pH 8. Enzyme activity was increased in the presence of MgSO4 and CaCl2. Amylase was stable with surfactants and commercial detergents for 30 min. Supplementation of the enzyme with commercial detergent improved the washing ability of the detergent. This investigation has revealed that these thermostable bacteria are excellent source of amylase which can be used commercially for generating economic activity on sustainable basis.

Seasonal shift in gut microbiome diversity in wild Sichuan takin (Budorcas tibetanus) and environmental adaptation

In this study, we investigated the change in microbiome composition of wild Sichuan takin (Budorcas tibetanus) during winter and spring and analyzed the physiological implications for such changes. Diversity analyses of the microbiome (average 15,091 high-quality reads per sample) in 24 fecal samples (15 from winter, 9 from spring) revealed that spring samples had higher species diversity and were compositionally different from winter samples (P < 0.05). Taxonomic composition analysis showed that the relative abundance increased in spring for Patescibacteria (2.7% vs. 0.9% in winter, P < 0.001) and Tenericutes (1.9% vs. 1% in winter, P < 0.05). Substantial increases in relative abundance of Ruminococcaceae and Micrococcaceae were identified in spring and winter, respectively. Mann-Whitney U and ANCOM identified seven differentially abundant genera: Enterococcus, Acetitomaculum, Blautia, Coprococcus 1, Lachnospiraceae UCG 008, Ruminococcus 2 and Ralstonia. All seven genera were significantly more abundant in spring (average 0.016–1.2%) than winter (average 0–0.16%), with the largest difference found in Ruminococcus (1.21% in spring vs. 0.16% in winter). The other six genera were undetectable in winter. Functional prediction and pathway analysis revealed that biosynthesis of cofactors (ko01240) had the highest gene count ratios in the winter, followed by the two-component system (ko02020). Seasonal variation affects the gut microbiomes in wild Sichuan takins, with winter associated with lower species diversity and spring with enrichment of cellulose-degrading genera and phytopathogens. Such changes were crucial in their adaptation to the environment, particularly the difference in food abundance.

Haze Exposure Changes the Skin Fungal Community and Promotes the Growth of Talaromyces Strains.

Haze pollution has been a public health issue. The skin microbiota, as a component of the first line of defense, is disturbed by environmental pollutants, which may have an impact on human health. A total of 74 skin samples from healthy students were collected during haze and nonhaze days in spring and winter. Significant differences of skin fungal community composition between haze and nonhaze days were observed in female and male samples in spring and male samples in winter based on unweighted UniFrac distance analysis. Phylogenetic diversity whole-tree indices and observed features were significantly increased during haze days in male samples in winter compared to nonhaze days, but no significant difference was observed in other groups. Dothideomycetes, Capnodiales, Mycosphaerellaceae, etc. were significantly enriched during nonhaze days, whereas Trichocomaceae, Talaromyces, and Pezizaceae were significantly enriched during haze days. Thus, five Talaromyces strains were isolated, and an in vitro culture experiment revealed that the growth of representative Talaromyces strains was increased at high concentrations of particulate matter, confirming the sequencing results. Furthermore, during haze days, the fungal community assembly was better fitted to a niche-based assembly model than during nonhaze days. Talaromyces enriched during haze days deviated from the neutral assembly process. Our findings provided a comprehensive characterization of the skin fungal community during haze and nonhaze days and elucidated novel insights into how haze exposure influences the skin fungal community. IMPORTANCE Skin fungi play an important role in human health. Particulate matter (PM), the main haze pollutant, has been a public environmental threat. However, few studies have assessed the effects of air pollutants on skin fungi. Here, haze exposure influenced the diversity and composition of the skin fungal community. In an in vitro experiment, a high concentration of PM promoted the growth of Talaromyces strains. The fungal community assembly is better fitted to a niche-based assembly model during haze days. We anticipate that this study may provide new insights on the role of haze exposure disturbing the skin fungal community. It lays the groundwork for further clarifying the association between the changes of the skin fungal community and adverse health outcomes. Our study is the first to report the changes in the skin fungal community during haze and nonhaze days, which expands the understanding of the relationship between haze and skin fungi.

Seasonal Changes in the Microbial Communities on Lettuce (Lactuca sativa L.) in Chungcheong-do, South Korea.

Lettuce is one of the most consumed vegetables worldwide. However, it has potential risks associated with pathogenic bacterial contamination because it is usually consumed raw. In this study, we investigated the changes in the bacterial community on lettuce (Lactuca sativa L.) in Chungcheong-do, South Korea, and the prevalence of foodborne pathogens on lettuce in different seasons using 16S rRNA gene-based sequencing. Our data revealed that the Shannon diversity index showed the same tendency in term of the number of OTUs, with the index being greatest for summer samples in comparison to other seasons. Moreover, the microbial communities were significantly different between the four seasons. The relative abundance of Actinobacteriota varied according to the season. Family Micrococcaceae was most dominant in all samples except summer, and Rhizobiaceae was predominant in the microbiome of the summer sample. At the genus level, the relative abundance of Bacillus was greatest in spring samples, whereas Pseudomonas was greatest in winter samples. Potential pathogens, such as Staphylococcus and Clostridium, were detected with low relative abundance in all lettuce samples. We also performed metagenome shotgun sequencing analysis on the selected summer and winter samples, which were expected to be contaminated with foodborne pathogens, to support 16S rRNA gene-based sequencing dataset. Moreover, we could detect seasonal biomarkers and microbial association networks of microbiota on lettuce samples. Our results suggest that seasonal characteristics of lettuce microbial communities, which include diverse potential pathogens, can be used as basic data for food safety management to predict and prevent future outbreaks.

Evaluation of efficiency, thermohydraulic performance evaluation criterion, and field synergy principle improvement of the parabolic solar collector containing the hybrid nanofluid using spring turbulators

In this paper, the study and simulation of turbulent flow inside a parabolic solar collector tube equipped with two spring insert samples with two different pitch ratios (P/D = 0.22, 0.44) and a specific cross-section are investigated. Heat transfer and properties of Cu–Fe3O4/Water hybrid nanofluid with volume fractions of φ = 1%, 3%, and 5% for the range of Reynolds numbers of 7000, 9000, and 11000 are selected and investigated using the single-phase method. The tube is used as the adsorbent pipe of a parabolic solar collector to investigate the performance of the parameters such as volume fraction, Nusselt number, pressure drop, parabolic solar collector efficiency, performance evaluation criterion, and field synergy principle. The results show that with decreasing the pitch ratio, Nu and solar collector efficiency increase. The maximum efficiency is 2.53 for a tube with P/D = 0.22 (Re = 7000, φ = 1%). The maximum efficiency is obtained under the same conditions in a tube containing the spring insert with P/D = 0.44 is equal to 2.39. At the same Reynolds numbers, the average of these velocities is significantly higher than a plain tube. Field Synergy Principle is a reliable criterion to investigate the mechanism of heat transfer increase that this coefficient value in the tube equipped with a spring insert is much higher than in a plain tube. This increase indicates the positive performance of the spring insert on the heat transfer rate and solar collector efficiency improvements. Therefore, spring inserts with a lower P/D are more desirable to achieve the highest efficiency of solar collectors as a source of renewable energy.

Spatial Distribution of Salmonella in Soil near Municipal Waste Landfill Site

Due to the heterogeneous origin of municipal waste, different substrates support the growth of many microorganisms, including those hazardous to humans. In consequence, landfills collecting these refuses are regarded as serious sources of infectious material contaminating the environment. In this study, we aimed to assess how waste may be related to the presence of Salmonella spp. in soil within a landfill and its surroundings. The numbers of these pathogens were estimated in soil samples collected at 17 different stands established in the municipal waste landfill of Barycz (near Kraków, Poland) and the surrounding area. The analysis showed that in all soil samples, Salmonella spp. did not exceed 270 cfu g−1 in dry soil (i.e., the active landfill sector). Salmonella spp. was found in 57% of the tested soil samples in spring, 88% in summer, 45% in autumn, and was not detected in winter. A spatial distribution visualized by graphical maps allowed determination of the influence of the active sector on the surrounding areas. The graphical maps showed the impact of seasons on the spread of Salmonella spp. in the soil near the landfill. Detection and estimation of Salmonella spp. distribution in soil within the landfill area distinctly confirms the hazardous impact of collected wastes on hygienic characteristics of the soil.

Microbial ecology of a shallow alkaline hydrothermal vent: Strýtan Hydrothermal Field, Eyjafördur, northern Iceland.

Strýtan Hydrothermal Field (SHF) is a submarine system located in Eyjafördur in northern Iceland composed of two main vents: Big Strýtan and Arnarnesstrýtan. The vents are shallow, ranging from 16 to 70 m water depth, and vent high pH (up to 10.2), moderate temperature (T max ∼70°C), anoxic, fresh fluids elevated in dissolved silica, with slightly elevated concentrations of hydrogen and methane. In contrast to other alkaline hydrothermal vents, SHF is unique because it is hosted in basalt and therefore the high pH is not created by serpentinization. While previous studies have assessed the geology and geochemistry of this site, the microbial diversity of SHF has not been explored in detail. Here we present a microbial diversity survey of the actively venting fluids and chimneys from Big Strýtan and Arnarnesstrýtan, using 16S rRNA gene amplicon sequencing. Community members from the vent fluids are mostly aerobic heterotrophic bacteria; however, within the chimneys oxic, low oxygen, and anoxic habitats could be distinguished, where taxa putatively capable of acetogenesis, sulfur-cycling, and hydrogen metabolism were observed. Very few archaea were observed in the samples. The inhabitants of SHF are more similar to terrestrial hot spring samples than other marine sites. It has been hypothesized that life on Earth (and elsewhere in the solar system) could have originated in an alkaline hydrothermal system, however all other studied alkaline submarine hydrothermal systems to date are fueled by serpentinization. SHF adds to our understandings of hydrothermal vents in relationship to microbial diversity, evolution, and possibly the origin of life.

Quantitative Assessment of Full Size Microplastics in Bottled and Tap Water Samples in Hong Kong.

Human exposure to microplastics (MPs) through drinking water has drawn serious concern recently because of the potential adverse health effects. Although there are reports on the occurrence of MPs in bottled water, little is known about the abundance of a whole spectrum of MPs with sizes ranging from 1 µm to 5 mm due to the restrictions of conventional MPs detection methods. Some studies using micro-Raman spectroscopy can achieve MPs with a size of <10 µm, however, quantitation of all MPs was extremely time consuming and only a small portion (<10%) of MPs would be analyzed. The present study quantified MPs from nine brands of bottled water using fluorescence microscopy and flow cytometry for MPs with a size of ≥50 µm and a size of <50 µm, respectively. The average abundance of MPs with a size of ≥50 µm in bottled water samples was found ranging from 8–50 particles L−1, while MPs with a size of <50 µm were found to be 1570–17,817 particles L−1, where the MPs abundance from mineral water samples were significantly more than distilled and spring water samples. The modal size and shape of MPs were found at 1 µm and fragments, respectively. Besides, three tap water samples obtained locally were analyzed and compared with the bottled water samples, where less MPs were found in tap water samples. In addition, contamination of MPs from bottle and cap and interference by addition of mineral salts were studied, where no significant difference from all these processes to the control sample was found, suggesting the major contamination of MPs was from other manufacturing processes. Estimated daily intake (EDI) of MPs increased substantially when data of small MPs are included, suggesting that previously reports on exposure of MPs from drinking water might be underestimated, as only large MPs were considered.