Sports Drinks Research Articles

Sports drinks effect on salivary volume and pH in children after exercise: a randomized clinical study.

The aim of this study was to examine the sports' related oral health behavior and the effect of sports drinks on the salivary volume and acidity of 6 to 14 years old children. Sixty-eight children with a median age of 8 years old from Waterford Tennis Association camp participated in the study. Each child was randomly assigned to either the control group that consumed water or the experimental group that consumed a sports drink. Salivary volume and acidity were measured for all groups before exercise, right after exercise and after consuming the rehydrating agent. Salivary volume was measured by dripping into a pre-measured (mL) plastic medicine cup while salivary acidity was measured using an electric pH meter with 0.01 sensitivity. Oral health behavior did not differ between the two groups. No statistically significant difference was detected in the salivary volume before and after exercise. A statistically significant increase (P=0.005) was found in the salivary volume before (1.73ml) and after re-hydration (2.92ml) regardless of the drink consumed (P=0.813). Salivary pH increased significantly (P=0.012) before (7.06) and after exercise (7.73), and dropped significantly (P=0.001) after the consumption of the rehydration drink (6.63) among the same group. The pH decrease was greater in the sports drink group (P=0.013). No difference in the children's salivary volume was found between the two groups. However, consumption of sports drinks reduces significantly salivary pH and thus, water should be the drink of choice for rehydration in children.

Hydration as a modulator of renal vascular control during physical work in the heat

Occupational heat stress increases the risk of developing kidney injury (KI), which is due to the development of hyperthermia and/or hypohydration. It is speculated that this KI is caused by a relative mismatch in oxygen supply (i.e., reductions in renal perfusion) and demand (due to increased sodium reabsorption) in the renal tubules. That said, the modulatory role of hydration on renal vascular control during physical work in the heat is largely unknown. Recent observations indicate that ad lib drinking largely prevents hypohydration during simulated occupational heat stress. Thus, we tested the hypothesis that, compared to ad libitum drinking, fluid restriction induces a pro-vasoconstrictor state in the renal vasculature during physical work in the heat.On two occasions, six healthy adults (1 woman) completed 2 h of exercise in a wet bulb globe temperature of 30±0°C, conditions that are commonly experienced by U.S. outdoor workers. Exercise consisted of completing eight circuits involving treadmill walking (10 min) and rowing (5 min) at a workload eliciting an oxygen uptake of 1.2 L/min, the work intensity observed during agriculture work. In the drinking trial (Drink), subjects were provided 237 mL of sport drink every 15 min and drank ad libitum, which is compliant with workplace hydration recommendations. In the fluid restriction trial (NoDrink), no fluid was provided. Rectal temperature and body mass loss were recorded in each trial. Blood pressure and right renal segmental blood velocity (BV, Doppler ultrasound) was measured before, and following 60 and 120 min of exercise. Before and following exercise, a cold pressor test (CPT) was used to assess the renal vasoconstrictor response to sympathetic activation by immersing the subject’s foot in an agitated ice slurry for 2 min. Blood pressure and segmental BV were measured during each minute of the CPT. Segmental artery vascular resistance (VR) was calculated as mean arterial pressure divided by segmental BV. Data presented as mean ± SD.Average rectal temperature was not different between Drink (37.8±0.4°C) and NoDrink (37.9±0.4°C, p=0.517). Body mass loss was greater in NoDrink (3.1±0.7%) vs. Drink (1.8±0.8%, p=0.014). Segmental BV did not change differently between trials (interaction: p=0.651). Segmental VR did not differ between trials before (Drink: 2.7±0.6, NoDrink: 2.5±0.6 mmHg/cm/s) or after 60 (Drink: 2.8±0.7, NoDrink: 2.6±0.6 mmHg/cm/s) and 90 (Drink: 2.5±0.3, NoDrink: 2.3±0.8 mmHg/cm/s) min of exercise (interaction: p=0.988). Peak increases in segmental VR during the CPT did not differ between trials before (Drink: 0.5 ± 0.7, NoDrink: 0.9 ± 0.6 mmHg/cm/s) or after (Drink: 0.4 ± 0.7, NoDrink: 0.4 ± 0.6 mmHg/cm/s) exercise (interaction: p=0.405).These findings preliminarily indicate that fluid replacement does not modify renal vascular control during physical work in the heat. NCT: NCT05458843. Supported by: R01OH011528 This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Reply to the Editor - Maternal inappropriate sinus tachycardia during pregnancy.

We would like to comment on the Letter to the Editor of Dr Wang on our manuscript, Management of Inappropriate Sinus Tachycardia during pregnancy (1Shah A.N. Ferreira S.W. Padanilam B.J. Prystowsky E.N. Management of inappropriate sinus tachycardia during pregnancy.Heart Rhythm O2. 2023; 4: 65-66https://doi.org/10.1016/j.hroo.2022.11.001Abstract Full Text Full Text PDF PubMed Scopus (0) Google Scholar). He acknowledges that we used the standard accepted definition of inappropriate sinus tachycardia (IST) but raises the issue of whether an alternative definition of IST should be used during pregnancy. Indeed, recent data in pregnant women show that median heart rate was 82 bpm at 12 weeks gestation and rose to 91 bpm at 34.1 weeks (2Green L.J. Mackillop L.H. Salvi M. et al.Gestation-specific vital sign reference ranges in pregnancy.Obstet Gynecol. 2020; 135: 653-664Crossref PubMed Scopus (42) Google Scholar). However, the average heart rates in our patients mostly recorded in the early 2nd trimester were typically more than 100 bpm, and more importantly they were highly symptomatic with the added history of rapid and marked increases in heart rate during exertion, a classic presentation of IST. They were referred for further evaluation because their physicians considered them to have something other than the usual mild increase in heart rate with pregnancy. Dr Wang comments on the use of metoprolol to treat these very symptomatic patients, and suggests that the data on its effectiveness in non-pregnant patients remains questionable. The senior author (ENP) has a different experience and has treated scores of IST patients prior to ivabradine availability with substantial success in many using metoprolol succinate. The limiting factor for success is often the inability to use a high enough dose because some of these patients simply cannot tolerate beta-blockade therapy even at a low dose. Further, it is our experience that volume expansion and use of salt supplementation, while valuable in autonomic syndromes of hypotension, are not effective in pure IST without hypotension. Last, although one would like to avoid the use of any drug during pregnancy, metoprolol is considered safe to use if needed (3Page R.L. Joglar J.A. Caldwell M.A. et al.2015 ACC/AHA/HRS Guideline for the management of adult patients with supraventricular tachycardia: a report of the American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines and the Heart Rhythm Society.Heart Rhythm. 2016; 13: e136-e221Abstract Full Text Full Text PDF PubMed Scopus (67) Google Scholar), and all our patients were treated beyond the first trimester. Risks and benefits of therapy were discussed during the shared decision making process, and all patients wanted to take metoprolol to suppress their marked symptoms. We agree that more data on IST in pregnancy, and the safety of ivabradine, would be useful and encourage others to investigate this problem. To the Editor – Maternal inappropriate sinus tachycardia during pregnancyHeart Rhythm O2PreviewShah and colleagues presented 11 patients with maternal inappropriate sinus tachycardia (IST) during pregnancy.1 Two major points are worth considering. Full-Text PDF Open Access

Investigating glycemic control in patients undergoing lower extremity bypass within an enhanced recovery pathway at a single institution

BackgroundEnhanced recovery pathways (ERPs) aim to lower perioperative stress to facilitate recovery. Limited fasting combined with carbohydrate loading is a common ERP element. The effect of limited fasting has not been elucidated in patients with diabetes. Given the known deleterious effects of poor glycemic control in the perioperative period, such as increased rates of surgical site infection, the associations of preoperative limited fasting with perioperative glycemic control and early outcomes after lower extremity bypass (LEB) were investigated. MethodsA single institutional retrospective review of patients who underwent infrainguinal LEB from 2016 to 2022 was performed. The ERP was initiated in May 2018. Patients were stratified by diabetes diagnosis and preoperative hemoglobin A1C (HbA1C) levels. Perioperative glycemic control was compared between the limited fasting and traditional fasting patients (nil per os at midnight). Limited fasting was defined as a clear liquid diet until 2 hours before surgery with recommended carbohydrate loading consisting of 400 cc of a clear sports drink (approximately 30 g of carbohydrates). All limited fasting patients were within the ERP. Early perioperative hyperglycemia (EPH) was defined as blood glucose of >180 mg/dL within the first 24 hours of surgery. Perioperative outcomes such as surgical site infection, readmission, reinterventions, and complications were also compared. ResultsA total of 393 patients were included (limited fasting patients N = 135; traditional fasting patients N = 258). A trend toward EPH was seen in all limited fasting groups. Evaluating limited fasting within diabetic patients revealed that 74.5% of limited fasting-diabetic patients had EPH compared with 49.6% of traditional fasting-diabetic patients (P = .001). When stratified by the HbA1C level, a significantly higher rate of EPH was seen in the HbA1c >8.0% groups, with 90.5% in the limited fasting patients compared with 67.9% in traditional fasting patients (P = .05). Limited fasting-diabetic patients experience a longer postoperative length of stay at 5.0 days (interquartile range: 3, 9) vs 4.0 days (2, 6) in nondiabetic patients (P = .016). ConclusionsERP limited fasting was associated with early perioperative hyperglycemia after LEB, particularly in patients with HbA1C >8.0%. Due to the high prevalence of diabetic patients undergoing LEB under ERP, the role of limited fasting and common glycemic elements of ERP may need to be re-evaluated in this subpopulation.

The effect of isotonic drink on heart rates recovery after pencak silat activity: a study on female students in an islamic boarding school

The aim of this study was to examine the effect of isotonic drinks on students’ heart rates. This experimental research relied on a randomized pretest–posttest control group design; it consisted of one control group (t1) and one treatment group (t2). All participants were treated with the same exercise protocol including endurance training specifically for the branches of pencak silat (exercise straight kick techniques, scythe while hitting). The control group consumed mineral water, while the treatment group was provided with an isotonic drink after exercise. Following the treatment phase was the calculation of participants’ heart rates for one minute; this process was replicated three times: before the exercise (T1), 60 to 90 minutes after the exercises (T2), and 10 minutes after the exercise interval (T3). All data were analyzed using a T-test. The results revealed that the initial heart rates of the control and treatment groups were 72.6 ± 10.36 and 66.3 ± 8.60, respectively. In other words, there were no differences between the two groups. The initial heart rates of the control group control and treatment group after the exercise was 159.2 ± 10.48 and 149.0 ± 8.59, respectively. According to the t-test results, there was a difference (p 0.05) in the heart rates of the control group and treatment group after the exercises. The initial heart rates of the control group control and treatment group after the exercise interval was 76.2 ± 11.0 and 65.9 ± 8.62, respectively. The t-test results reported a difference (p 0.05) in the heart rates of the control group and treatment group after the exercise interval of 10 minutes. Isotonic drinks significantly to heart rates recovery after pencak silat exercise of female students.

Porous Copper Oxide Thin Film Electrodes for Non-Enzymatic Glucose Detection

The present work describes novel copper oxide thin film-modified indium tin oxide electrodes prepared by magnetron sputtering and their application for glucose sensing. Copper oxide-modified sensors were characterized by electrochemical techniques, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM). The deposited thin film (of about 400 nm of thickness) consisted of Cu2O/CuO nanocolumns of ca. 80 nm in diameter. After optimizing the main experimental parameters, the electrodes showed noteworthy electrocatalytic properties for glucose detection (sensitivity ca. 2.89 A M−1 cm−2 and limit of detection ca. 0.29 μM (S/N = 3)). The sensor showed negligible response against common electroactive species and other sugars. Finally, recovery experiments in commercial soda drinks and the determination of glucose content in different commercial drinks, such as soda, tea, fruit juices, and sports drinks, are described.

Modulation of gut microbiota and intestinal immune response in gilthead seabream (Sparus aurata) by dietary bile salt supplementation.

Given their role in lipid digestion, feed supplementation with bile salts could be an economic and sustainable solution to alterations in adiposity and intestinal inflammation generated by some strategies currently used in aquaculture. An important part of the metabolism of bile salts takes place in the intestine, where the microbiota transforms them into more toxic forms. Consequently, we aimed to evaluate the gut immune response and microbial populations in gilthead seabream (Sparus aurata) fed a diet supplemented with a blend of bile salts with proven background as a regulator of lipid metabolism and fat content. After the 90-day feeding trial, a differential modulation of the microbiota between the anterior and posterior intestine was observed. While in the anterior intestine the relative abundance of Desulfobacterota doubled, in the posterior intestine, the levels of Firmicutes increased and Proteobacteria, Actinobacteriota, and Campylobacterota were reduced when supplementing the diet with bile salts. Even so, only in the anterior intestine, there was a decrease in estimated richness (Chao1 and ACE indices) in presence of dietary bile salts. No significant differences were displayed in alpha (Shannon and Simpson indices) nor beta-diversity, showing that bile sales did not have a great impact on the intestinal microbiota. Regarding the gene expression profile in 2 h postprandial-fish, several changes were observed in the analyzed biomarkers of epithelial integrity, nutrient transport, mucus production, interleukins, cell markers, immunoglobulin production and pathogen recognition receptors. These results may indicate the development of an intestinal immune-protective status to tackle future threats. This work also suggests that this immune response is not only regulated by the presence of the dietary bile salts in the intestine, but also by the microbial populations that are in turn modulated by bile salts. After a fasting period of 2 days, the overall gene expression profile was stabilized with respect to fish fed the unsupplemented diet, indicating that the effect of bile salts was transient after short periods of fasting. On the balance, bile salts can be used as a dietary supplement to enhance S. aurata farming and production without compromising their intestinal health.

Erosive Potential of Various Beverages in the United Arab Emirates: pH Assessment

Objectives: The United Arab Emirates (UAE) is one of the top 5 countries in the world in terms of juice consumption per capita. The rising consumption of beverages is a major contributor to a plethora of diseases, including heightened risks of developing obesity, diabetes, and osteoporosis. Beverages with low pH also tend to magnify the risk of long-term dental erosion. This paper evaluated the pH of various beverages in the UAE to help determine their erosive potential. Materials and Methods: 306 beverages were collected from different stores in the UAE. Drinks were categorized into 8 categories: carbonated drinks, energy drinks, sparkling water, iced teas, juices, malt beverages, coconut water, and sports drinks. Three readings were taken for each drink using a pH meter at room temperature. Results: 88% of all beverages had a pH of less than 4.0, and the remaining 12% had a pH of 4.0 or more. 37% of the beverages tested were extremely erosive (pH < 3.0), 51% were erosive (pH 3.0–3.99), and 12% were mildly erosive (pH ≥ 4.0). Bickford’s Classic Cola and Tesco Lemon Juice were the most acidic beverages (pH < 2.4). The most identified acids in the tested beverages were citric, phosphoric, ascorbic, and malic acids. Conclusions: The majority of beverages in the UAE are erosive to dentition as the pH is below 4. Future studies to raise public awareness on the effect of acidic drinks on tooth structure are needed.

Fluid balance during physical work in the heat is not modified by the menstrual cycle when fluids are freely available.

We tested the hypothesis that women may be more at risk of becoming dehydrated during physical work in the heat in the early follicular phase (EF), compared with the late follicular (LF) and mid-luteal (ML) phases of the menstrual cycle when allowed free access to drink. Twelve healthy, eumenorrheic, unacclimated women (26 ± 5 yr) completed three trials (EF, LF, and ML phases) involving 4 h of exposure to 33.8 ± 0.8 °C, 54 ± 1% relative humidity. Each hour, participants walked on a treadmill for 30 min at a rate of metabolic heat production of 338 ± 9 W. Participants drank a cool, flavor-preferred non-caloric sport drink ad libitum. Nude body weight was measured pre- and post-exposure, and percent changes in body weight loss were interpreted as an index of changes in total body water. Total fluid intake and urine output were measured and sweat rate was estimated from changes in body mass corrected for fluid intake and urine output. Fluid intake was not different between phases (EF: 1,609 ± 919 mL; LF: 1,902 ± 799 mL; ML: 1,913 ± 671; P = 0.202). Total urine output (P = 0.543) nor sweat rate (P = 0.907) differed between phases. Percent changes in body mass were not different between phases (EF: -0.5 ± 0.9%; LF: -0.3 ± 0.9%; ML: -0.3 ± 0.7%; P = 0.417). This study demonstrates that the normal hormonal fluctuations that occur throughout the menstrual cycle do not alter fluid balance during physical work in the heat.NEW & NOTEWORTHY The effect of the menstrual cycle on fluid balance during physical work in the heat when fluids are freely available is unknown. This study demonstrates that fluid balance is not modified in women across three distinct phases of the menstrual cycle during physical work in the heat These results indicate that when women have free access to cool fluid during physical work in the heat, they respond similarly across all three phases to maintain fluid homeostasis across the menstrual cycle.

THE EFFECT OF GLYCERIN APPLICATION ON ROUGHNESS OF NANOHYBRID COMPOSITE RESIN IMMERSED IN ISOTONIC DRINK

ABSTRACTBackground: Composite resin is the most commonly used restorative material. One type of composite resin based on the filler material is a nanohybrid composite resin which has the advantage of good strength and smooth surface to produce good improvements in terms of durability and aesthetics. The roughness of the resin can be affected by drinks with low pHs, such as isotonic drink. Glycerin can reduce the roughness of the composite resin by acting as a barrier so that an oxygen inhibition layer (OIL) is not formed. Purpose: This study is to determine the effect of glycerin application on the roughness of nanohybrid composite resin immersed in an isotonic drink. Method: A total of 32 samples of nanohybrid composite resin used in this study were made with a diameter of 5 mm and a thickness of 2 mm. The nanohybrid composite resin samples were divided into group I without glycerin application and group II with glycerin application before the light curing process. Both groups were then immersed in an isotonic drink for 18 hours in an incubator. The research continued with roughness testing using a surface roughness tester. Result: There was a significant difference in the roughness of the nanohybrid composite resin immersed in an isotonic drink with and without glycerin application. This is indicated by the p-value &lt; 0.05 in the unpaired bivariate t-test, which means that there is a significant difference in the two sample groups. Conclusion: The application of glycerin can reduce the roughness of the nanohybrid composite resin immersed in isotonic drink. Keywords: Glycerin application, Nanohybrid composite resin, Roughness.

Disinfection and Isotonic Drinks' Influence on Hardness and Color Stability of Ethylene-Vinyl-Acetate Copolymer Mouthguards Used in Martial Arts: An In Vitro Study.

This in vitro study aimed to evaluate the hardness and color change of an ethylene-vinyl-acetate copolymer (EVA) material for mouthguards after exposition to different cleaning agent solutions and isotonic drinks. Four hundred samples were prepared and divided into four equinumerous groups (n = 100), in which there were 25 samples from each color of EVA (red, green, blue and white). The hardness, using the digital durometer, and the color coordinates (CIE L*a*b*), using the digital colorimeter, were measured before the first exposition and after 3 months of exposition to spray disinfection and incubation in the oral cavity temperature, or immersion in isotonic drinks. The values of Shore A hardness (HA) and color change (ΔE-calculated by Euclidean distance) were statistically analyzed using the Kolmogorov-Smirnov test, multiple comparison ANOVA/Kruskal-Wallis and appropriate post-hoc tests. Statistically significant changes in color and hardness between the tested groups were demonstrated after the use of agents predestined for disinfecting the surface of mouthguards on the tested samples. There were no statistically significant differences in color and hardness between the groups immersed in isotonic sport drinks potentially consumed by competitors practicing combat sports using mouthguards. Despite the changes in color and hardness after the use of disinfectants, the deviations were minor and limited to specific colors of the EVA plates. The intake of isotonic drinks practically did not change either the color or the hardness of the samples, regardless of the tested color of the EVA plates.

Dietary intake of household cadmium-contaminated rice caused genome-wide DNA methylation changes on gene/hubs related to metabolic disorders and cancers

Cadmium (Cd) contamination in food has raised broad concerns in food safety and human health. The toxicity of Cd to animals/humans have been widely reported, yet little is known about the health risk of dietary Cd intake at the epigenetic level. Here, we investigated the effect of a household Cd-contaminated rice (Cd-rice) on genome-wide DNA methylation (DNAm) changes in the model mouse. Feeding Cd-rice increased kidney Cd and urinary Cd concentrations compared with the Control rice (low-Cd rice), whereas supplementation of ethylenediamine tetraacetic acid iron sodium salt (NaFeEDTA) in the diet significantly increased urinary Cd and consequently decreased kidney Cd concentrations. Genome-wide DNAm sequencing revealed that dietary Cd-rice exposure caused the differentially methylated sites (DMSs), which were mainly located in the promoter (32.5%), downstream (32.5%), and intron (26.1%) regions of genes. Notably, Cd-rice exposure induced hypermethylation at the promoter sites of genes Caspase-8 and interleukin-1β (Il-1β), and consequently, their expressions were down-regulated. The two genes are critical in apoptosis and inflammation, respectively. In contrast, Cd-rice induced hypomethylation of the gene midline 1 (Mid1), which is vital to neurodevelopment. Furthermore, ‘pathways in cancer’ was significantly enriched as the leading canonical pathway. Supplementation of NaFeEDTA partly alleviated the toxic symptoms and DNAm alternations induced by Cd-rice exposure. These results highlight the broad effects of elevated dietary Cd intake on the level of DNAm, providing epigenetic evidence on the specific endpoints of health risks induced by Cd-rice exposure.

Comparison of Natural Mineral Water From Agrı Province With Sports Drinks

Dehydration emerges due to lack of fluid balance in the body. In addition, minerals such as sodium, potassium, calcium, and magnesium are lost because of dehydration. Athletes lose fluids and electrolytes through sweating during exercise, and replacing the lost fluid and electrolytes is crucial for the continuity of their sports performance. Sports drinks can be consumed by athletes to replace fluid and electrolytes lost during exercise and to improve performance. During this period, consumption of sports drinks minimizes dehydration by restoring the lost mineral and fluid balance. However, the composition of these products, their active ingredients, their effects on sports performance, and their risk factors are matters of debate. This study was carried out in order to compare sports drinks with the local mineral water that comes out naturally in the province of Agri. The mineral determinations of the local mineral water were made using Inductively Coupled Plasma-Mass Spectroscopy (ICP- MS) instruments. As a result of the analyses, the average sodium (Na) value in the local mineral water was 26.6 ppm, and the magnesium (Mg) average was 7.782 ppm. No potassium (K) mineral was detected in Agri local mineral water. Calcium (Ca) mineral average was found to be 65.04 ppm, iron (Fe) mineral average 0.0068 ppm, and zinc (Zn) mineral average 0.02457 ppm. Agri natural mineral water contains minerals in similar amounts to sports drinks. Considering these values, it can be a natural alternative to sports drinks if potassium mineral and glucose is added to its content, so it can be an alternative to sports drinks.

Prevalence and attitudes towards nutritional supplements use among gymnasium goers in Eldoret Town, Kenya

Active people in sports or regular exercises make up a significant portion of individuals using nutritional supplements (NS). Therefore the purpose of this study was to investigate the prevalence of NS (types, sources of information), motives for going to the gym, reasons for consumption of nutritional supplements and attitudes towards nutritional supplements among gymnasium users in Eldoret Town, Kenya. Data was collected using a questionnaire from 210 participants who were sampled through systematic convenience sampling from 11 gyms in Eldoret Town, Kenya. The study found that majority (55.3%) of the respondents used nutritional supplements and most of them used the supplements believing that they enhance their performance at the gym and therefore perceived usage of supplements as acceptable. The major reasons for going to gym were to enhance health, body building and to stay fit. Most (91%) of the gym users got the information about NS from the internet and the most consumed NS were protein supplements (72%), followed by sport drinks (69%) and fish oils (60%). It is concluded that gym goers use nutritional supplements and have positive attitudes towards their use. Therefore, this study recommends that users should be sensitized on the value, precautionary measures and side effects of using nutritional supplements.

The Impact of Mineral and Energy Supplementation and Phytogenic Compounds on Rumen Microbial Diversity and Nitrogen Utilization in Grazing Beef Cattle

The objective of this study was to evaluate the effect of the addition of a phytogenic compound blend (PHA) containing hydrolyzable tannins, carvacrol, and cinnamaldehyde oil to mineral salt or energy supplementation on the rumen microbiota and nitrogen metabolism of grazing Nellore cattle. Eight castrated Nellore steers were distributed in a double-Latin-square 4 × 4 design, with a 2 × 2 factorial arrangement (two types of supplements with or without the addition of the PHA), as follows: energy supplement without the PHA addition (EW); energy supplement with the PHA addition (EPHA); mineral supplement without the addition of the PHA (MW); mineral supplement with the PHA addition (MPHA). Steers that received supplements with the PHA have a lower ruminal proportion of valerate (with the PHA, 1.06%; without the PHA, 1.15%), a lower ruminal abundance of Verrucomicrobia, and a tendency for lower DM digestibility (with the PHA, 62.8%; without the PHA, 64.8%). Energy supplements allowed for higher ammonia concentrations (+2.28 mg of NH3-N/dL), increased the propionate proportion (+0.29% of total VFA), and had a higher ruminal abundance of Proteobacteria and Spirochaetae phyla in the rumen. The PHA addition in the supplement did not improve nitrogen retention, reduced the ruminal proportion of valerate, and had a negative impact on both the total dry-matter digestibility and the abundance of several ruminal bacterial groups belonging to the Firmicutes and Verrucomicrobia phyla.

Predicting Rates of Angular Correction After Hemiepiphysiodesis in Patients With X-Linked Hypophosphatemic Rickets.

Patients with X-linked hypophosphatemic rickets (XLH) often develop coronal plane knee deformities despite medical treatment. Hemiepiphysiodesis is an effective way to correct coronal plane knee deformities in skeletally immature patients, but a full understanding of the rate of angular correction after hemiepiphysiodesis in XLH patients, compared with idiopathic cases is lacking. We retrospectively reviewed charts of 24 XLH patients and 37 control patients without metabolic bone disease who underwent hemiepiphysiodesis. All patients were treated with standard-of-care medical therapy (SOC=active vitamin D and phosphate salt supplementation) in our clinical research center and had a minimum of 2-year follow-up after hemiepiphysiodesis. Demographic data as well as complications, repeat procedures, or recurrence/overcorrection were recorded. Standing lower extremity radiographs were evaluated before the surgical intervention and at subsequent hardware removal or skeletal maturity, whichever came first. Mean axis deviation, knee zone, mechanical lateral distal femoral angle (mLDFA), and medial proximal tibial angle were measured on each radiograph. The rate of angular correction was calculated as the change in mLDFA and medial proximal tibial angle over the duration of treatment. The magnitude of the initial deformity of the distal femur was greater in XLH patients as compared with control for varus (XLH mLDFA 97.7 +/- 4.9 vs. Control mLDFA 92.0 +/- 2.0 degrees) and valgus (XLH mLDFA 78.7 +/- 6.2 vs. Controls mLDFA 83.6 +/- 3.2 degrees). The rate of correction was dependent on age. When correcting for age, XLH patients corrected femoral deformity at a 15% to 36% slower rate than control patients for the mLDFA (>3y growth remaining XLH 0.71 +/- 0.46 vs. control 0.84 +/- 0.27 degrees/month, <3y growth remaining XLH 0.37 +/- 0.33 vs. control 0.58 +/- 0.41 degrees/month). No significant differences were seen in the rate of proximal tibia correction. XLH patients were less likely to end treatment in zone 1 (55.0% XLH vs. 77.8% control). XLH patients had longer treatment times than controls (19.5 +/- 10.7 vs. 12.6 +/- 7.0mu, P value <0.001), a higher average number of secondary procedures than controls (1.33 +/- 1.44 vs. 0.62 +/- 0.92 number of procedures), a higher rate of overcorrection than controls (29.2% vs. 5.4%), and a higher rate of subsequent corrective osteotomy than controls (37.5% vs. 8.1%). There was no significant difference in the rate of complications between groups (8.3% vs. 5.4%). Patients with XLH undergoing hemiepiphysiodesis have a 15% to 36% slower rate of femoral deformity correction that results in longer treatment times, a higher likelihood to undergo more secondary procedures, and a lower likelihood to reach neutral mechanical alignment. This study provides important information to guide the timing and treatment of patients with XLH and coronal plane knee deformities. In addition, results from this study can be educational for families and patients with respect to anticipated treatment times, success rates of the procedure, complication rate, and likelihood of needing repeat procedures.

COMPARISON BETWEEN SPORTS DRINK AND COCONUT WATER ON CARDIOVASCULAR ENDURANCE PERFORMANCE AMONG FOOTBALL PLAYERS

A sports drink is a drink containing electrolytes and glucose, meanwhile coconut drink is known as a natural source of sports drink. Both drinks have the benefit of restoring energy and electrolyte, especially when ingested during strenuous physical activity that requires maximum endurance performance. The purpose of this study was to compare the effects of sports drinks and coconut drinks in enhancing cardiovascular endurance performance among UiTM football players. A total of 24 male participants representing Universiti Teknologi MARA Football Club (UiTM FC) were recruited. Participants were randomly assigned into three groups that are isotonic drink group (IDG) (n=8), the coconut drink group (CDG) (n=8), and the control group (n=8). Cardiovascular endurance performance was determined based on bleep test results at the pre-test and post-test. The intervention was conducted by giving a different type of drink according to the assigned group. IDG received 100 plus isotonic drink, CDG received a Vita Coco drink and CG were only given plain water. After the end of the pre-test session, the participants were given 2-hour to rest, and drinks were given according to their group. After 2-hour of rest, the participants continue with the post-test. The level of VO2 max was measured by an equation based on the bleep test results. The result shows there was a significant increment in pre-post-test VO2 max level within all groups, showing improvement in the mean level of VO2 max at post-test. The mean of pre-post-test VO2 max for the three respective groups was IDG 47.53 ± 6.32 to 49.46 ± 5.88 ml/kg/min, CDG 47.01 ± 3.45 to 48.81 ± 3.29 ml/kg/min and CG 47.06 ± 3.11 to 47.91 ± 3.43 ml/kg/min. However, there was no significant difference (p=0.276) between groups and pre-post-test VO2 max level. Looking at between groups effect, there was no significant effect (p=0.900) between these three groups and their VO2 max level. In conclusion, there was a significant positive change in the participant's pre-post-test VO2 max level for all three groups across two different periods at the pre-test and post-test. This suggests ingesting drinks between exercises substantially improved football players' cardiovascular endurance performance. However, there was no significant difference in the participants' cardiovascular endurance performance between the three groups. This indicates the type of drink does not influence the cardiovascular endurance performance among these football players.

Dental Erosion and Diet in Young Children and Adolescents: A Systematic Review

Dental erosion is the irreversible loss of tooth enamel caused by acid attack without any bacterial involvement. Interest in dental erosion has increased considerably during the past decade. This paper aims to determine the prevalence of and association of dental erosion with diet or beverages in children. A systematic review of the literature has been carried out in accordance with the PRISMA recommendations. A search was performed in PubMed, Embase Scopus and Web of Science databases, and completed manually. Those studies that dealt with erosive risk factors related to diet and that included their effect on deciduous or mixed dentition were selected. Dual independent screening, data extraction and risk of bias assessment were concluded. A total number of 708 articles were identified from the initial electronic search of the four databases. Some 466 articles were selected after duplicated articles were discarded. After reading the title and abstract, we eliminated 376 articles; the remaining 90 were meant to be read in order that we could dispatch the ones that did not answer the research question or the inclusion criteria. A total of 25 articles remained for the systematic review. Erosive lesions are favored by the consumption of carbonated and isotonic drinks, fruit juices and acid sauces. Carbonated, isotonic drinks and fruit drinks favor the development of erosive lesions, not so the consumption of fruit juices mixed with dairy products such as milkshakes, yogurts and/or tea. The findings of this article will help researchers, academics and students to characterize the scientific results regarding dental erosion and diet, to evaluate management strategies and to identify significant topics and questions that will help to design future research with the aim of the prevention of the disease. Registration number: CDR42023389750.

Do Spanish Triathletes Consume Sports Supplements According to Scientific Evidence? An Analysis of the Consumption Pattern According to Sex and Level of Competition.

The use of sports supplements (SS) to improve sports performance is very common in athletes. In the case of triathletes, the physiological characteristics of the sport may require the use of certain SS. Although the consumption of SS is widespread in this sport, very few studies have investigated it thus far. The aim is to analyze the pattern of SS consumption by triathletes according to sex and the competitive level. This is a descriptive cross-sectional study on the consumption and habitual use of SS of 232 Spanish-federated triathletes. Data were collected through a validated questionnaire. Overall, 92.2% of the athletes consumed SS, but no significant differences were found in terms of competition level or sex. Yet, significant differences were found regarding the level of competition for total SS (p = 0.021), the total number of Group A supplements from the AIS classification (p = 0.012), and for the ergogenic aids (p = 0.003). The most-consumed SS were bars, sports drinks, sports gels, and caffeine (83.6%, 74.1%, 61.2%, and 46.6%, respectively). The consumption of SS by triathletes is high, and the number of SS consumed rises from the regional to the national and international levels. The four SS most consumed were included in category A of the AIS (greatest scientific evidence).

Development and Physico-chemical Evaluation of an Isotonic Nas Narang (Citrus madurensis) Sports Drink

Isotonic drinks are the type of sports drink with a similar number of osmotically active particles as present in the body plasma (i.e. osmolality; 275-295 mOsmole/kg) and help for replenishing the loss of electrolytes and sugar after physical workout. The present study describes the potential of utilization of Nas Narang (Citrus madurensis) fruit juice in the development of Isotonic Nas Narang Sports Drink (INNSD) and investigation of the physico-chemical parameters. INNSD was developed using varying content of fresh juice of Nas Narang 5%, 6.5%, and 7.5% v/v with other recommended food grade ingredients. The 7.5% of fresh Nas Narang juice was selected as the best fruit content based on the highest overall acceptability in sensorial evaluation using a trained sensory panel. The total osmolality of developed isotonic Nas Narang sports drink was 282.95 mOsmol/l. The physical properties of Total Soluble Solids content (TSS), Titratable Acidity (TA), and pH of the INNSD were 9.00±0.00%, 0.53±0.01%, and 2.45±0.01 respectively. The proximate composition of Nas Narang fruit juice and developed sports drink were total ash (0.47±0.11%; 0.42±0.17%), protein (0.47±0.01%; 0.05±0.00%), crude fibre (0.53±0.01%; 0.09±0.01%), total carbohydrate content (6.97±0.27%; 7.31±0.31%) and caloric value (29.44 Kcal /per 100 ml of sports drink) respectively. The vitamin contents of INNSD were ascorbic acid (0.3 mg/100 ml), vitamin B2 (0.135 mg/100 ml), vitamin B5 (0.15mg/100ml) and vitamin B6 (0.145 mg/100 ml). Further, anti-oxidant potential of Total Polyphenolic Content (TPC) and Total Flavonoids Content (TFC) of fresh Nas Narang fruit juice and developed INNSD were (0.224±0.02 mg GAE/ml; 0.029±0.00 mg GAE/ml) and (0.139±0.01 mg QE/ml; 0.011±0.00 mg QE/ml) respectively.&#x0D; Nas narang fruit is available throughout the year as an adequate source of raw material for industry. It provides natural citrus flavor, attractive yellow colour, appealing taste, minerals and antioxidants to the product and further act as a natural preservative. Present study showed incorporation of Nas Narang juice in formulation of sport drink is technically sound and acceptable.