Two field experiments were carried out at Kalabsho Experimental Farm, Dakahlia Governorate, Sugar Crops Research Institute, Agricultural Research Center, during 2009/2010 and 2010/2011 seasons, to evaluate the effect of nitrogen fertilizer levels (100, 120, and 140 kg N/fad), three split applications of nitrogen fertilizer (two, three and four equal doses) and harvesting dates (180, 195 and 210 days after sowing) on productivity and quality of sugar beet cv. Gazelle. Increasing nitrogen fertilizer levels from 100 to 140 kg N/fad significantly increased root fresh weight, root length and diameter, root and sugar yields/fad in both seasons. The inverse was true in total soluble solids (TSS), sucrose and purity percentages in both seasons. Splitting nitrogen fertilizer from 2 to 4 equal doses significantly increased root fresh weight, root length and diameter, TSS %, purity % and root and sugar yields/fad in both seasons. On the other hand, it decreased sucrose % in both seasons. Delaying harvesting date from 180 to 210 days after sowing significantly increased all studied characters, except for root juice purity in both seasons. Splitting 140 kg N/fad into four equal doses and harvesting at 210 days after sowing are the suitable treatment to maximize sugar beet productivity and quality under the environmental conditions of the newly reclaimed sandy soils of Kalabsho region, Dakahlia Governorate.