This paper delves the structural and magnetic properties of Mn-doped Dy0.4Sm0.6Fe1-xMnxO3 (x = 0, 0.1, 0.2 and 0.3) orthoferrite. With an increase in Mn concentration, the a and c lattice parameters show a decreasing trend, while the b parameter shows a linear increase and an variance is observed in Fe–O–Fe and Fe–O-Sm bond angles and bond lengths, indicating a distortion in the FeO6 octahedra. FESEM images show that as the Mn content increases, the grain size increases and porosity decreases. EDX analysis confirms that, the prepared samples are high in purity and the elemental composition. The Dy0.4Sm0.6FeO3 (i.e., x = 0.0) orthoferrite demonstrates a spin reorientation transition at 234K. With an increase in Mn doping at the Fe site induces a complex magnetic structure, Mn/Fe3+-O-Mn/Fe3+ interactions weaken the antiferromagnetic nature thereby decreasing spin reorientation transition (SRT) temperature. The moderate Mn doping at the Fe site leads to a canted-like antiferromagnetic structure, playing a crucial role in tuning the magneto-electric properties at room temperature.