The interaction of toxic metal exposure and oxidative balance score on semen quality: A cross-sectional study based on Shanghai volunteers, China.

Effects of graded dietary black soldier fly larvae powder inclusion on semen quality and serum biochemical indices of Luxi game breeder roosters.

Presence of sperm-bound antisperm antibodies and their association with semen quality in dogs.

Impact of cold storage on semen quality in two divergent rabbit lines selected for resilience under heat stress condition.

Artificial intelligence-based prediction of boar reproductive fitness and health: Current status in research and practice.

Epimedium polysaccharide alleviates adenine-induced oligoasthenozoospermia in mice by inhibiting ferroptosis and inflammation through TLR4/NF-κB and SLC7A11/GPX4 axis.

The ginkgo biloba extract (GBE) alleviates the cryoinjury of porcine sperm via the NRF2/AKT/ERK signaling.

Proteomic insights into the effects of cryopreservation on sperm of endangered Eld's deer (Rucervus eldii).

A multi-omics atlas of testicular development in Leiocassis longirostris: dynamic regulation of spermatogenesis.

Wild fish populations face growing threats from anthropogenic activities, climate change, and natural disasters. Aquatic germplasm banking is a vital strategy for preserving genetic diversity and supporting sustainable aquaculture. This study proposes a sequential, decision-guided framework for developing species-specific sperm cryopreservation protocols, applied here to the endangered Pogonias courbina, listed as endangered in Brazil and vulnerable by the IUCN. A series of experiments was conducted to identify optimal conditions for thawing (26°C for 14s), equilibration time (10min), extenders (HBSS, 3% glucose, 5% glucose), and both permeable (dimethyl sulfoxide, propylene glycol, dimethylformamide, ethylene glycol) and non-permeable cryoprotectants (glucose, fructose, sucrose, trehalose, skimmed powdered milk, egg yolk). Sperm quality was assessed using Computer-Assisted Sperm Analysis (CASA). Three treatments with the highest post-thaw motility (MOT) were selected to fertilize oocytes from two hormonally-induced females. No significant difference was observed between sperm from wild and farmed males. The best kinetic parameters and fertilization rates were obtained using the combination of HBSS +10% DMSO +5% egg yolk (MOT: 71.8%). Based on these findings, we propose practical recommendations regarding extender selection, equilibration time, cryoprotectant combinations, and thawing conditions that support high post-thaw viability and successful fertilization. This is the first study to report successful artificial reproduction of P. courbina using cryopreserved sperm, establishing a reproducible protocol that can be applied for captive breeding, restocking, and germplasm banking. The proposed framework offers a generalizable approach for developing cryopreservation protocols in fish species.

Cryoprotective effects of mangiferin-loaded chitosan nanoparticles on buffalo sperm: Antioxidant, apoptotic, and motility insights.

Male fertility is declining globally, prompting efforts to identify contributing dietary factors. Wheat, a major staple worldwide, provides caloric and protein value but also contains anti-nutritional components such as wheat germ agglutinin (WGA), which has been implicated in oxidative stress and infertility. This study investigated the effects of common wheat lectin on male reproductive parameters in prepubertal Sprague-Dawley (SD) rats. Forty-five prepubertal SD rats (40-50 g; 30 days old) were randomized into three treatment durations (2, 5, and 10 weeks), each subdivided into three groups: control, pure wheat lectin (250 mg/kg), and common wheat lectin extract (250 mg/kg). Treatments were administered orally once daily. At each time point, testicular histology, sperm quality, reproductive hormones, and oxidative stress markers were assessed. Rats treated with wheat lectin showed significant reductions (p < 0.05) in testicular antioxidants (SOD, CAT, GSH) and testosterone (serum and intratesticular), alongside elevated malondialdehyde (MDA) and reproductive hormones (LH, FSH, prolactin). Sperm concentration, motility, and morphology were significantly impaired. Histological analysis revealed notable damage to the seminiferous germinal epithelium, expansion of the interstitial compartment consistent with edema, and basement membrane disintegration. Chronic exposure to wheat-derived lectins, may adversely influence male reproductive biology in this prepubertal rat model. The findings suggest a potential association between lectin exposure and testicular dysfunction, possibly mediated by alterations in hormone regulation and oxidative stress. These results should be interpreted cautiously and warrant further investigation to clarify their relevance to human reproductive health.

Enhanced endocrine-metabolic support and axonemal assembly in high-sperm-motility geese: insights from testicular cellular heterogeneity by scRNA-seq.

Effect of seasons on cryopreserved sperm quality and subsequent fertility in Ouled Djellal rams.

Testicular aging revisited: Current understanding of mechanisms and emerging interventions.

Integrative assessment of gamete and embryo quality in porcine in vitro production: A comprehensive review.

HSP72 alleviates heat stress-induced oxidative damage in boar Sertoli cells through modulating the AGE-RAGE/NOX4/NF-κB axis.

Proteomic analysis of spermathecal fluid in Amphioctopus fangsiao: molecular insights into long-term sperm storage.

Cordycepin-mediated protection against bisphenol A-induced male reproductive toxicity via PI3K/mTOR/HIF-1α signaling.

Artificial insemination (AI) is commonplace in commercial pig breeding, and as such, ensuring sperm sample quality is of utmost importance to avoid reduced farrowing rates and litter sizes. Here, we have used high-throughput nuclear morphometric analysis to compare pig sperm samples categorised as meeting the AI standard (AIS) or not meeting the AI standard (N-AIS) (50 vs. 44 samples from unique boars, respectively). We show that pig sperm nuclei are asymmetric, that samples contain phenotypic shape abnormalities that show continuous variation and that samples classed as N-AIS have more abnormally shaped sperm than AIS samples. This variation is not detected in conventional analysis. The specific phenotypes identified suggest the disruption of key aspects of spermiogenesis and indicate future avenues for improving pig sperm quality. Additionally, we find that the distribution and severity of abnormal sperm phenotypes were significantly associated with the day of semen collection, indicating that environmental factors may influence sperm morphology. High-throughput nuclear morphometric analysis therefore reveals significant differences in sperm head morphology between AIS and N-AIS pig sperm samples and has the potential to be further developed as a tool for sperm assessment both in the pig breeding industry and in other species.