Specific Ingredients Research Articles

A Comprehensive Analysis of Volatile and Nonvolatile Components in Berberis fortunei and Its Inhibition Against HT29 Colorectal Cancer Cells Through GC-IMS, LC-QTOF-MS, and Docking-Based Network Analysis.

Berberis fortunei Lindl. (BF) is a medicinal plant widely utilized in East Asia. However, the chemical components present in its roots, stems, and leaves have not been systematically analyzed and compared. The specific active ingredients that inhibit HT29 colorectal cancer cells are still unclear. The aim of this study is to comprehensively analyze the chemical compositions of BF's roots, stems, and leaves and to evaluate their biological function against HT29 cells. GC-IMS and LC-QTOF-MS were employed to analyze the volatile and nonvolatile components of BF, respectively. The MTT assay was used to evaluate the inhibitory effects of extracts and compounds from BF on HT29 cells. A network analysis based on molecular docking was conducted to identify the potential targets of compounds. A total of 77 volatile components and 116 nonvolatile components were identified in the roots, stems, and leaves of BF. The inhibitory activity of different parts of BF against HT29 cells followed the order: roots > stems > leaves. Protoberberine-type alkaloids showed more pronounced effects at 24 h, whereas bisbenzylisoquinoline-type alkaloids demonstrated stronger activity at 48 h. Network analysis based on molecular docking revealed significant differences in the pathways targeted by the two types of alkaloids. This study not only comprehensively analyzed the compositions of BF but also examined its biological function in inhibiting HT29 cells, laying a theoretical foundation for its further development and application. The findings provide diverse lead compounds for the subsequent development of drugs against colorectal cancer.

Houshiheisan modulates the NF-κB/MLCK signaling pathway to protect the endothelial barrier in cerebral small vessel disease.

Houshiheisan modulates the NF-κB/MLCK signaling pathway to protect the endothelial barrier in cerebral small vessel disease.

Ultra-Processed Foods and Type 2 Diabetes Mellitus: What Is the Evidence So Far?

Ultra-processed foods (UPFs) are foods that have undergone extensive industrial processing with the addition of various substances in order to make them more tasty, eye-catching, and easy to consume. UPFs are usually rich in sugars, salt, and saturated fat, whereas they lack essential nutrients. The aim of this review is to elaborate upon the current evidence associating overconsumption of UPFs with the development of type 2 diabetes mellitus (T2DM). We will discuss data interconnecting UPFs and T2DM risk and will further describe specific ingredients that have been suggested to increase this risk. In addition, we will thoroughly explain how additives, such as emulsifiers or sweeteners, or other compounds formed during manufacturing, such as acrylamide and acrolein, and during packaging, such as bisphenol-A, are proposed to be implicated in the pathogenesis of insulin resistance and T2DM.

Impacts of Personalized Multimodal Treatment in the Clinical Management of Androgenetic Alopecia: A Case Report

Introduction: Androgenetic alopecia (AGA) is one of the most common forms of hair loss, characterized by the progressive miniaturization of hair follicles. The condition affects both men and women and has a significant impact on patients' self-esteem and psychological well-being. The treatment of AGA remains challenging, with available therapeutic options that do not always result in satisfactory outcomes. Methodology: This case study investigated the efficacy of a multimodal treatment for AGA, combining oral therapy, at-home topical care, microinfusion of specific active ingredients, and photobiomodulation. The patient was treated with Actrisave, Saw Palmetto, Trichoxidil, and microinfusion of growth factors such as KGF and copper peptide. The treatment was carried out over a determined period, with clinical and photographic follow-up throughout the sessions. Results and Conclusion: The patient showed a significant improvement in hair density and scalp coverage. The treatment resulted in an increase in the anagen phase of the hair cycle, a reduction in the telogen phase, and greater formation of hair follicle units. Additionally, no adverse effects were reported, and the patient expressed satisfaction with the results achieved. The combination of oral, topical treatments, and microinfusion of active ingredients, along with photobiomodulation technologies, proved effective in the treatment of androgenetic alopecia, promoting hair regeneration and increased hair density. The personalized approach, which considers the specific needs of each patient, was crucial for the success of the treatment. However, further studies are needed to confirm the efficacy of these treatments on a larger scale and in the long term.

Predictive Modeling of Glycemic Indices of Value-Added Coconut (Cocos nucifera L) Jaggery Enriched with Specific Ingredients

Predictive Modeling of Glycemic Indices of Value-Added Coconut (Cocos nucifera L) Jaggery Enriched with Specific Ingredients

Integrated Risk Framework (IRF)—Interconnection of the Ishikawa Diagram with the Enhanced HACCP System in Risk Assessment for the Sustainable Food Industry

This paper presents a new risk assessment methodology called the Integrated Risk Framework (IRF) through the application of Ishikawa diagrams combined with the enhanced Hazard Analysis and Critical Control Point (HACCP) system. This risk investigation technique aims to ensure a significantly higher level of quality, safety, and sustainability in food products by using improved classical methods with strong intercorrelation capabilities. The methodology proposes expanding the typology of basic physical, chemical, and biological risks outlined by the ISO 22000 Food Safety Management System standard, adding other auxiliary risks such as allergens, fraud/sabotage, Kosher/Halal compliance, Rapid Alert System for Food and Feed notification, or additional specific risks such as irradiation, radioactivity, genetically modified organisms, polycyclic aromatic hydrocarbons, African swine fever, peste of small ruminants, etc. depending on the specific technological process or ingredients. Simultaneously, it identifies causes for each operation in the technological flow based on the 5M diagram: Man, Method, Material, Machine, and Environment. For each identified risk and cause, its impact was determined according to its severity and likelihood of occurrence. The final effect is defined as the risk class, calculated as the arithmetic mean of the impact derived at each process stage based on the identified risks and causes. Within the study, the methodology was applied to the spring water bottling process. This provided a new perspective on analyzing the risk factors during the bottling operations by concurrently using Ishikawa diagrams and HACCP principles throughout the product’s technological flow. The results of the study can form new methodologies aimed at enhancing sustainable food safety management strategy. In risk assessment using these two tools, the possibility of cumulative or synergistic effects is considered, resulting in better control of all factors that may affect the manufacturing process. This new perspective on studying the dynamics of risk factor analysis through the simultaneous use of the fishbone diagram and the classical HACCP system can be extrapolated and applied to any manufacturing process in the food industry and beyond.

Simultaneous Determination of Stilbenes, Flavones, Coumestans, Anthraquinones, and Chalcones in Ethanolic Extract of Pet-Sang-Kard Mixed Herbal Remedy Using HPLC-PDA Analysis.

The Pet-Sang-Kard mixed herbal remedy (PSKMHR) is a traditional Thai medicinal formulation used as an herbal supplement for the treatment of hemorrhoids. This remedy consists of four specific herbal ingredients in the following proportions: 50 parts Cissus quadrangularis L. stems, 15 parts Eclipta prostrata L. aerial parts, 10 parts Rheum sp. rhizome, and 10 parts Boesenbergia rotunda (L.) Mansf. rhizome. This study presents the development, validation, and application of a high-performance liquid chromatography with photodiode array detection (HPLC-PDA) method designed for the simultaneous quantification of 13 key bioactive compounds, including rhaponticin, rhapontigenin, quercitrin, wedelolactone, aloe-emodin, rhein, emodin, chrysophanol, physcion, alpinetin, pinocembrin, pinostrobin, and panduratin A, present in the 70% ethanolic extract of PSKMHR. Method validation was conducted in accordance with Association of Official Analytical Collaboration (AOAC) international guidelines, evaluating parameters such as the specificity, linearity, accuracy, precision, and limit of detection. The results demonstrated exceptional linearity (R > 0.9999), high precision (% RSD < 2), and recovery rates within acceptable limits (98-102%) for all analytes. This developed method was successfully applied to quantify the 13 target compounds in the crude extracts of PSKMHR formulated from 10 market raw material samples, providing a robust analytical framework for quality control of this herbal remedy.

Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients.

Although various plant protein (PP) ingredients are available on the market, their application in foods is not trivial, and food companies are struggling to identify PP ingredients fitting the intended use. To fill this gap, abundant literature has appeared but data are hardly comparable due to the absence of a recognized classification of PP ingredients accounting not only for protein purity but also for the process history, and of standardised protocols for technological functionality assessment. In this review, a comprehensive analysis of comparable literature data was thus carried out to elucidate the effect of composition and processing variables on PP technological functionalities. The review presents four sections describing: (i) the approach followed for the construction of a database of PP ingredient functionalities; (ii) the composition and processing factors relevant to PP ingredients; (iii) PP ingredient functional properties and methods used for their determination; (iv) the effect of composition and processing factors on PP ingredient functionalities. This analysis showed legume proteins to present the highest solubility and interfacial properties while pseudocereal ones the highest water-holding capacity. Although pure ingredients show higher functionalities, non-protein components could contribute to interfacial properties. Alkaline extraction, isoelectric precipitation and freeze-drying is the process mostly used in academic research to obtain PP ingredients. However, other extraction, purification, and drying methods can be properly combined, resulting in specific PP ingredient functionalities. Overall, this review highlights that, besides protein purity and source, knowledge of the processing history is required to select PP ingredients with desired functionalities.

A bibliometric and visualization analysis of the role of traditional Chinese medicine in cancer immunotherapy.

Traditional Chinese medicine (TCM) is used as a complementary treatment for patients with cancer, especially in immunotherapy. Although extensive clinical and basic research has been conducted on TCM in cancer immunotherapy, a comprehensive bibliometric analysis of this field has not yet been performed. This study aimed to investigate the progress and status of TCM, and the research focused on cancer immunotherapy. We collected 1,657 articles on TCM in cancer immunotherapy from 1994 to 2024 from the Web of Science Core Collection database. VOSviewer, CiteSpace, and the Bibliometrix R package were used to analyze countries, institutions, journals, authors, references, and keywords to predict future trends in cancer immunotherapy with TCM. The publication rate of TCM in cancer immunotherapy research steadily increased from 1994 to 2018, with a swift growth from 2018 to 2023. China and TCM universities have achieved the most research advancements in this field. The most studied types of cancer are liver, lung, and colorectal cancers. However, few studies exist on upper respiratory tract tumors, cervical cancer, and melanoma, which deserve more attention. The study trend has gradually shifted from in vivo and in vitro models to clinical efficacy. Simultaneously, the focus of research transitioned from compound TCM preparations or classes of ingredients to specific pharmacodynamic ingredients, and the corresponding targets transitioned from cytokines to immune checkpoints. In general, molecular docking combined with multi-omics analysis is a popular and trending research method in TCM for cancer immunotherapy, helping researchers understand the mechanisms of TCM in cancer immunotherapy more comprehensively and accurately. By analyzing the literature, it is evident that TCM-based immunotherapy should contribute to effective maintenance or adjuvant therapy throughout the entire course of cancer rather than only in the late stages. This study comprehensively summarized and identified research frontiers providing a reference for promoting the development of TCM immunotherapy preparations and guiding clinical practice. Consequently, more patients with cancer can benefit from immunotherapy.

International Symposium on Ruminant Physiology: Paternal Nutrient Supply: Impacts on Physiological and Whole Animal Outcomes in Offspring.

Recent evidence suggests that environmental factors experienced by sires can be transmitted through the ejaculate (seminal plasma + sperm) into the female reproductive tract, influencing fertilization, embryo development, and postnatal offspring outcomes. This concept is termed paternal programming. In rodents, sire nutrition was shown to directly alter offspring outcomes through sperm epigenetic signatures, DNA damage/oxidative stress, cytokine profiles, and/or the seminal microbiome. Response variables impacted in rodent models, including adiposity, muscle mass, metabolic responses, and reproductive performance, could have major productivity and financial implications for producers if these paternal programming responses are also present in ruminant species. However, a paucity of data exist regarding paternal programming in ruminants. The limited data in the literature mainly point to alterations in sperm epigenome as a result of sire diet or environment. Global nutrition has been implicated in ruminant models to alter seminal cytokine profiles, which could subsequently alter the uterine environment and immune response to mating. Several reports indicate that embryo development and epigenetic signatures can be impacted by sire plane of nutrition and inclusion of specific feed ingredients into diets (polyunsaturated fatty acids, folic acid, and rumen protected methionine). Models of sheep nutrition indicate that addition of rumen protected methionine can impact DNA methylation and offspring performance characteristics extending to the F3 generation, and that divergent planes of sire nutrition can cause altered hormone profiles and insulin/glucose metabolism in offspring. There are almost unlimited opportunities for discovery in this area, but researchers are encouraged to target critical questions such as whether and the extent to which paternal programming effects are present in common management scenarios, the mechanisms by which paternal programming is inherited in ruminants, and whether the effects of paternal nutrition interact with those of maternal nutrition to influence offspring physiology, whole animal outcomes, and herd or flock productivity.

Immunonutrition in Operated-on Gastric Cancer Patients: An Update.

Enteral immune nutrition has attracted considerable attention over the past few years regarding its perioperative role in patients undergoing major surgery for digestive cancer. Today, the term enteral immune nutrition refers to the perioperative administration of nutritional preparations containing, among others, specific ingredients such as glutamine, omega-3 polyunsaturated fatty acids, and arginine. They provide nutritional support and exert pharmacological effects through the substances contained in these preparations. Their administration to patients with gastric cancer is necessary as malnutrition and other metabolic disorders are frequent symptoms with effects on the level of immune responses, affecting the function of intestinal permeability and, therefore, the effectiveness of chemotherapy. Existing clinical data and data from all meta-analyses published so far support the view that enteral immune nutrition enhances the immune responses of gastric cancer patients, and reduces the rate of postoperative complications, and the duration of hospitalization without, however, improving patient survival. The content of enteral immune nutrition, dose, administration interval, and the effect on patient survival should be more precisely determined through relevant extensive multicenter studies. This systematic review describes and analyses the clinical results and the findings of relevant meta-analyses of the application of enteral immune nutrition in gastric cancer patients, emphasize the importance of this therapeutic intervention for disease progression, and attempts to provide practical guidelines for applying enteral immune nutrition in daily clinical practice.

Therapeutic effects of Huangqi formula (Eefooton) in chronic kidney disease: clinical research and narrative literature review.

The study aimed to assess the clinical effects of employing the Huangqi formula (Eefooton; EFT) for chronic kidney disease (CKD) treatment. A narrative literature review was undertaken to elucidate the specific ingredients of EFT and their potential impact on renal health. A retrospective observational study investigated EFT treatment in outpatients with stable CKD (stages 3B to 5) from March 2019 to March 2021. Patients received 20 mL of EFT thrice daily for 6 months, along with standard treatment. Control groups were matched to the EFT cohort. Regular assessments of renal, liver functions, and lipid profiles were conducted. Serum creatinine (Cr) and eGFR levels consistently improved in stage 3B CKD patients at each follow-up visit. At 6 months, improvement in Cr and eGFR was observed for stage 4 and 5 CKD. Stage 3B CKD patients exhibited notable reductions in systolic blood pressure after 3 and 6 months of EFT treatment. Remarkably, a substantial decrease in HbA1C was noted in stage 4 CKD individuals after three months of therapy. Additionally, stage 4 CKD patients saw a significant reduction in LDL levels after both 3 and 6 months of EFT treatment. A literature review on EFT ingredients indicated that the positive effects of EFT might be associated with its anti-inflammatory, antioxidant, and anti-fibrotic properties. This research demonstrated that incorporating EFT alongside standard treatment enhanced renal function in individuals with CKD. EFT is proposed as a feasible complementary treatment for CKD patients, emphasizing the importance of early intervention.

Perspectives of traditional herbal medicines in treating retinitis pigmentosa.

Medicinal plants, also known as herbs, have been discovered and utilized in traditional medical practice since prehistoric times. Medicinal plants have been proven rich in thousands of natural products that hold great potential for the development of new drugs. Previously, we reviewed the types of Chinese traditional medicines that a Tang Dynasty monk Jianzhen (Japanese: Ganjin) brought to Japan from China in 742. This article aims to review the origin of Kampo (Japanese traditional medicine), and to present the overview of neurodegenerative diseases and retinitis pigmentosa as well as medicinal plants in some depth. Through the study of medical history of the origin of Kampo, we found that herbs medicines contain many neuroprotective ingredients. It provides us a new perspective on extracting neuroprotective components from herbs medicines to treat neurodegenerative diseases. Retinitis pigmentosa (one of the ophthalmic neurodegenerative diseases) is an incurable blinding disease and has become a popular research direction in global ophthalmology. To date, treatments for retinitis pigmentosa are very limited worldwide. Therefore, we intend to integrate the knowledge and skills from different disciplines, such as medical science, pharmaceutical science and plant science, to take a new therapeutic approach to treat neurodegenerative diseases. In the future, we will use specific active ingredients extracted from medicinal plants to treat retinitis pigmentosa. By exploring the potent bioactive ingredients present in medicinal plants, a valuable opportunity will be offered to uncover novel approaches for the development of drugs which target for retinitis pigmentosa.

Mediterranean Lifestyle: More Than a Diet, A Way of Living (and Thriving).

Although the Mediterranean Diet (MedDiet) may appear simple and locally inspired based on the provided definitions, mounting evidence reveals that adopting a dietary pattern aligned with MedDiet principles can yield numerous health benefits. Also, the MedDiet stands as the gold standard in preventive medicine, so it is not a simplistic diet centered around specific ingredients or cooking methods; instead, it emerges from a distinctive way of life (lifestyle) inherent to the Mediterranean region. This lifestyle encapsulates essential components of a balanced diet and life, including frugality and moderation in food consumption, the utilization of seasonal and traditional products, a focus on locally sourced, eco-friendly, and biodiverse items, personal engagement in culinary preparation, the emphasis on conviviality and social activities during meal consumption, regular engagement in physical activity, adequate hydration, and sufficient rest. In this review, we will establish the interconnections and links between the various aspects of the Mediterranean diet, how these pillars reflect the Mediterranean region's distinctive lifestyle, and how each element is a necessary part of the others. Finally, the seamless integration of social involvement, sufficient rest, regular physical exercise, and diet will be explored to provide a holistic view of the Mediterranean lifestyle and its inherent harmony.

Perspective: Revisiting the Concepts of Prebiotic and Prebiotic Effect in Light of Scientific and Regulatory Progress – A Consensus Paper from the Global Prebiotic Association (GPA)

The term prebiotic has been used for almost three decades and has undergone numerous updates over the years. The scientific literature reveals that despite continuous efforts to establish a globally unified definition to guide jurisdictional regulations and product innovations, ambiguity continues to surround the terms prebiotic and prebiotic effect, leading to products that lack in full regulatory adherence being marketed worldwide. Thus, to reflect the current state of scientific research and knowledge and for the continuous advancement of the category, an update to the current prebiotic definition is warranted. This update includes removing the term selectivity, considering additional locations of action besides the gut, highlighting prebiotic performance benefits such as cognitive and athletic, and providing a clear standalone definition for prebiotic effect. The Global Prebiotic Association (GPA) is a leading information and industry hub committed to raising awareness about prebiotics, their emerging and well-established health benefits, and prebiotic product integrity and efficacy. In this position paper, GPA builds on previous prebiotic definitions to propose the following expanded definition for prebiotic: “a compound or ingredient that is utilized by the microbiota producing a health or performance benefit.”In addition to prebiotic, GPA also defines prebiotic effect as: “a health or performance benefit that arises from alteration of the composition and/or activity of the microbiota, as a direct or indirect result of the utilization of a specific and well-defined compound or ingredient by microorganisms.” With these two definitions, GPA aims to paint a clearer picture for the term prebiotic, and by incorporating an industry point of view, these updated definitions may be used alongside current scientific and regulatory perspectives to move the category forward. Statement of SignificanceThe purpose of this paper is to revisit the concepts of prebiotic and prebiotic effect by providing a scientific-based industry perspective. The proposed definitions of prebiotic and prebiotic effect reflect the recent discoveries in metagenomics and prebiotic research after the International Scientific Association for Probiotics and Prebiotics’ (ISAPP’s) 2017 prebiotic definition and propose terminology changes that are timely and necessary. These changes aim to maintain the clarity and usefulness of the prebiotic definition to the scientific community, industry, healthcare providers, and consumers, while ensuring scientific validity, comprehensiveness, and justification of each part of the prebiotic definition, including abandoning the term selectivity and introducing concepts of performance benefits and prebiotic effect.

Connecting polyphenols and myofibrillar proteins with their bioactive potentials: a terse review

Abstract The functional properties of animal food protein, especially from both nutritional and health contexts, remain very crucial to (food) product formulation/processing. Moreover, the increases in consumer awareness continues to push the food industry to seek alternatives of chemical food preservatives, particularly those natural approaches able to extend the shelf life of animal (food) product(s). As such, plant-derived bioactive substances occupy an important space as promising additives for animal food products. Besides, the underlying mechanisms that advances the interactive progress of antioxidant/bioactive compounds within the plant food matrices remains a continued debate. Indeed, to understand how myofibrillar proteins interact with the specific bioactive ingredients, i.e. biologically active compounds of plant origin, is of great importance especially their antioxidants/microbial potentials operating as food additives given the eventual impact to modify the functionality/sensory features of the emergent animal food products. To supplement existing information, therefore, this terse review synthesizes some connections between polyphenol and myofibrillar proteins derived from plant-based sources, with emphasis on their anti-microbial potentials. Overall, natural food additives may offer potential health benefits, but their usage comes with challenges, the latter which animal food producers must carefully consider particularly the effectiveness of bioactive substances on (animal food) products’ safety, and consumer appeal.

Sustainability and nutritional aspects of traditional dishes from the Friuli Venezia Giulia region, Italy: A true cost accounting evaluation

Sustainability and nutritional aspects of traditional dishes from the Friuli Venezia Giulia region, Italy: A true cost accounting evaluation

IngredSAM: Open-World Food Ingredient Segmentation via a Single Image Prompt.

Food semantic segmentation is of great significance in the field of computer vision and artificial intelligence, especially in the application of food image analysis. Due to the complexity and variety of food, it is difficult to effectively handle this task using supervised methods. Thus, we introduce IngredSAM, a novel approach for open-world food ingredient semantic segmentation, extending the capabilities of the Segment Anything Model (SAM). Utilizing visual foundation models (VFMs) and prompt engineering, IngredSAM leverages discriminative and matchable semantic features between a single clean image prompt of specific ingredients and open-world images to guide the generation of accurate segmentation masks in real-world scenarios. This method addresses the challenges of traditional supervised models in dealing with the diverse appearances and class imbalances of food ingredients. Our framework demonstrates significant advancements in the segmentation of food ingredients without any training process, achieving 2.85% and 6.01% better performance than previous state-of-the-art methods on both FoodSeg103 and UECFoodPix datasets. IngredSAM exemplifies a successful application of one-shot, open-world segmentation, paving the way for downstream applications such as enhancements in nutritional analysis and consumer dietary trend monitoring.

Lipid metabolism and food ingredients from the perspective of thermogenic adipocytes.

The high heat-producing capacity of brown and beige adipocytes, collectively known as thermogenic adipocytes, contributes to whole-body energy expenditure and is an attractive target for the management of obesity. It has been revealed that the functions of thermogenic adipocytes are important for the regulation of whole-body carbohydrate and lipid metabolism, and the activation of thermogenic adipocytes seems to have beneficial effects for the management of obesity-related metabolic disorders, such as dyslipidemia. Recent studies have showed that specific food ingredients have the potential to activate thermogenic adipocytes via various mechanisms. Some of these are effective not only in rodents, but also in humans, and effective prevention of obesity using these food ingredients is expected. In this review, we introduce the recent findings on the regulatory mechanisms of lipid metabolism by thermogenic adipocytes and food ingredients, demonstrating the potential to activate thermogenic adipocytes and their underlying mechanisms.

Nano to micron-size combustion particles in smokers’ homes: Magnetic properties of tobacco and cigarette ashes

Nano to micron-size combustion particles in smokers’ homes: Magnetic properties of tobacco and cigarette ashes