Species Of Other Families Research Articles

Strategy to Conserve Maximum Biodiversity of Limestone Flora in Peninsular Malaysia

The need to exploit limestone products for national development impacts on the conservation of rare and endangered limestone species. To minimise this impact it is necessary to identify which of the 570 limestone outcrops have high conservation importance and whether they have narrowly endemic and/or endangered limestone species. In the absence of detailed checklists for each outcrop, the Gesneriaceae is selected as being biodiverse on limestone, being well collected and outstanding in the number of endemic species and/or species restricted to limestone for mapping. Of the 210 species in the 25 genera of Gesneriaceae in Peninsular Malaysia, 42 species belonging to the genera Damrongia Kerr ex Craib, Emarhendia Kiew, A.Weber & B.L.Burtt, Epithema Blume, Microchirita (C.B.Clarke) Yin Z.Wang, Monophyllaea R.Br., Ornithoboea Parish ex C.B.Clarke, Paraboea (C.B.Clarke) Ridl., Senyumia Kiew, A.Weber & B.L.Burtt and Spelaeanthus Kiew, A.Weber & B.L.Burtt grow on limestone hills. Of these 42 species, 28 are endemic and 16 are restricted to a single or adjacent hills. Mapping their distribution shows that in common with other families there are three distinct phytogeographical provinces (the Northern Province, Perak Province and the Asian Intrusion) but that the narrowly endemic species do not cluster on particular hills, nor does their distribution coincide with those of other narrowly endemic species in other families. This illustrates that a network comprising a large number of hills, not only those within the three provinces but also all those harbouring narrowly endemic species that are at risk of extinction if not conserved, is necessary to maximise conservation of the biodiversity of the limestone flora.

Does lack of pollination extend flower life?

Across angiosperm species, the longevity of individual flowers can range from fixed to highly plastic. The orchid family is noteworthy for frequent reports of species in which flower lifespans are greatly prolonged if flowers are not pollinated. Less dramatic cases of pollination-induced senescence of anthesis have been reported for various species in other families, but such reports are scattered. Frequently, such findings are peripheral components of more general pollination studies. Because pollination-dependent plasticity can ameliorate phenological dislocations between plants and pollinators, it is worthwhile to conduct systematic surveys of its magnitude and taxonomic distribution. As a start, we report a set of experiments comparing the active lifespans of pollinated flowers to those of unpollinated controls in a set of nine species from a local subalpine flora. In all species, unpollinated flowers had longer mean times of receptiveness than pollinated ones, although the differences in means were often small. Three species exhibited significantly extended floral longevity in the absence of pollination.

Diversity of Diptera families that pollinate Ceropegia (Apocynaceae) trap flowers: An update in light of new data and phylogenetic analyses

Pollination by flies (Diptera) has been important to the diversification and ecology of the flowering plants, but is poorly understood in contrast to pollination by other groups such as bees, butterflies and birds. Within the Apocynaceae the genera Ceropegia and Riocreuxia temporarily trap flies, releasing them after a fixed, species-specific period of time, during which pollination and/or pollen removal occurs. This “trap flower” pollination system shows convergent evolution with unrelated species in other families and fascinated Stefan Vogel for much of his career, leading to ground-breaking work on floral function in Ceropegia (Apocynaceae). In this new study we extend the work of the latest broad analysis published by some of the authors (Ollerton et al., 2009 − Annals of Botany). This incorporates previously unpublished data from India and Africa, as well as recently published information, on the diversity of pollinators exploited by Ceropegia. The analyses are based on a more accurate phylogenetic understanding of the relationships between the major groups, and significantly widens the biogeographic scope of our understanding of fly pollination within Ceropegia. Information about the pollinators of 69 taxa (species, subspecies and natural varieties) of Ceropegia is now available. Twenty five families of Diptera are known to visit the flowers of Ceropegia, of which sixteen are confirmed as pollinators. Most taxa are pollinated by species from a single family. Overall, there were no major biogeographic differences in the types of Diptera that were used in particular regions, though some subtle differences were apparent. Likewise there were no differences between the two major clades of Ceropegia, but clear differences when comparing the range of Diptera exploited by Ceropegia with that of the stapeliads. This clade, one of the largest in the Asclepiadoideae, is a fascinating example of a species radiation driven by an apparently relatively uniform set of pollinators.

National carbon model not sensitive to species, families and site characteristics in a young tropical reforestation project

Reforestation and restoration offer critical contributions to addressing climate change and biodiversity decline. Enabling carbon credits to be derived from these activities is important for reforestation, particularly since reforestation does not come cheaply. Australia’s Carbon Farming Initiative is a world-leading policy that allows carbon credits to be obtained by using published methods-based approaches. Here we apply two different approaches to a young mixed species reforestation project in the wet tropics of Queensland, Australia. One approach assesses carbon sequestration from published allometric equations requiring direct field measurements, and the other applies a national carbon accounting model, FullCAM.Using allometric equations, we found above-ground biomass was influenced significantly by family, species, size class, and the interaction of family and size class. Species in the family Proteaceae out-performed species in other families. Selection of species according to soil nutrient status could enhance growth rates, but if soil nutrients and species responses are not known, then a bet-hedging strategy using mixed species from a variety of families is probably the best option.For three year old forest plots, FullCAM modelled significantly more carbon mass of trees than published allometric models for mixed tropical forests, suggesting that FullCAM needs adjustment to more accurately reflect species, families, local conditions and small-scale sites.Current policy settings are at odds with the needs of carbon farmers, considering the importance of forests and landscape restoration in fighting climate change and biodiversity decline. Legislated national methods allowing the development of species-specific allometrics for small mixed plantations do not account for the costs of developing these allometrics, especially in markets that are marginal.

Molecular organization of 5S rDNA in two ukrainian populations of sycamore (Acer pseudoplatanus)

Aim. The genetic variability of tree species has still not been studied enough at the molecular level. Considering the relatively low rate of molecular evolution in perennial tree species, it is necessary to use only those regions of genome, which demonstrate a high level of variability, such as 5S rDNA. Accordingly, to estimate the potential of 5S rDNA as a molecular marker for tree species, the organization of this genomic region was compared between samples from two geographically remote Ukrainian populations of sycamore, Acer pseudoplatanus. Methods. PCR amplification, cloning and sequencing of the 5S rDNA IGS of A.pseudoplatanus. Results. It was shown that only one variant of 5S rDNA repeat with a length of 475 bp is present in the genome of A. pseudoplatanus. Also, it was found that the elements typical for the angiosperm RNA polymerase III promoter, which are localized in IGS, appear to be different from those previously described for species of other families. The level of IGS sequence similarity within the populations exceeds 99%, while the level of IGS sequence similarity between various populations is only 93,3-94,3%. Conclusions. High rate of molecular evolution of the 5S rDNA IGS makes them a convenient molecular marker for evaluation of intraspecific variation in populations of A. pseudoplatanus.Key words: 5S rDNA, molecular markers, intraspecific variability, Acer

Ultraviolet-B-induced DNA damage and ultraviolet-B tolerance mechanisms in species with different functional groups coexisting in subalpine moorlands.

High doses of ultraviolet-B (UV-B; 280-315 nm) radiation can have detrimental effects on plants, and especially damage their DNA. Plants have DNA repair and protection mechanisms to prevent UV-B damage. However, it remains unclear how DNA damage and tolerance mechanisms vary among field species. We studied DNA damage and tolerance mechanisms in 26 species with different functional groups coexisting in two moorlands at two elevations. We collected current-year leaves in July and August, and determined accumulation of cyclobutane pyrimidine dimer (CPD) as UV-B damage and photorepair activity (PRA) and concentrations of UV-absorbing compounds (UACs) and carotenoids (CARs) as UV-B tolerance mechanisms. DNA damage was greater in dicot than in monocot species, and higher in herbaceous than in woody species. Evergreen species accumulated more CPDs than deciduous species. PRA was higher in Poaceae than in species of other families. UACs were significantly higher in woody than in herbaceous species. The CPD level was not explained by the mechanisms across species, but was significantly related to PRA and UACs when we ignored species with low CPD, PRA and UACs, implying the presence of another effective tolerance mechanism. UACs were correlated negatively with PRA and positively with CARs. Our results revealed that UV-induced DNA damage significantly varies among native species, and this variation is related to functional groups. DNA repair, rather than UV-B protection, dominates in UV-B tolerance in the field. Our findings also suggest that UV-B tolerance mechanisms vary among species under evolutionary trade-off and synergism.

Hydration status influences seed fire tolerance in temperate European herbaceous species.

Prescribed burning is an important management tool in many parts of the world. While natural fires generally occur during the driest and warmest period of the year, prescribed burning is often timed out-of-season, when there is higher soil moisture and lower biomass combustibility. However, fire season may influence seedling recruitment after fire, e.g. through the effect of seed hydration status on fire tolerance. In non-fire-prone temperate regions, anthropogenic fire may occur exclusively in periods outside the growing season with higher soil moisture, which may have negative consequences on seedling recruitment. Fire tolerance of moist and dry seeds of 16 temperate European herbaceous species belonging to four families was assessed using heat treatment of 100°C for 5min and subsequent germination trials. Moist seeds of Asteraceae, Poaceae and Brassicaceae had a predominantly negative reaction to the heat treatment, while those of Fabaceae tolerated it or germination was even enhanced. The reaction of dry seeds was completely different, with positive responses in three species of the Fabaceae and fire tolerance in species of other families. Our results point out that hydration status may significantly influence the post-fire germination of seeds. Dry seeds were found to tolerate high heat, while moist seeds were harmed in more than half of the species. This implies that if prescribed burning is applied in temperate grasslands of Europe, it should be timed to dry periods of the dormant season in order to protect seeds from negative effects of fire.

Stem anatomy of cipó-caboclo (Davilla rugosa, Dillenianecae) in Rio de Janeiro, Brazil

In Brazilian folk medicine, stems of Davilla rugosa, locally known as cipo-caboclo, are known for their anti-inflammatory and antiulcer uses, as well as their use as a laxative, stimulant, aphrodisiac, and tonic. The plant is also important in religious rituals. Previous studies showed that the stem contains flavonoids and terpenes. The ethnomedicinal use of D. rugosa has been reported all over Brazil. Plants of the genus Davilla are used to make safe herbal medicines with only few and insignificant side effects. In Rio de Janeiro, Brazil, six species of Davilla occur: Davilla glaziovii, Davilla grandifolia, Davilla latifolia, Davilla nitida, D. rugosa, and D. tintinnabulata. The stems of all these species have very similar external morphology. We emphasize that D. glaziovii is an endangered species and must not be sold. The stems of cipo-caboclo are sold in major herb markets in Rio de Janeiro State and open-air street markets in the City of Rio de Janeiro. The present study aimed at assessing the stem anatomy of cipo-caboclo sold commercially in these markets to unveil which species are being traded under this name. We conclude that some of the species sold are of the genus Davilla, but it is not possible to separate them at species level. We also found species of the families Aristolochiaceae and Bignoniaceae. These results are alarming, as the possibility of selling either D. glaziovii or species of other families as cipo caboclo can represent a risk to consumer health. Key words: Anatomy, cipo-caboclo, Davilla rugosa, Dilleniaceae, medicinal plants.

Two new and two redescribed species of Anonchotaenia (Cestoda: Paruterinidae) from South American birds

Morphological examination of novel specimens of paruterinid cestodes from passerine birds from Brazil and Chile and of museum specimens from Paraguay revealed two new species: Anonchotaenia prolixa sp. n. from Elaenia albiceps chilensis Hellmayr from Chile, and Anonchotaenia vaslata sp. n. from Tyrannus melancholicus (Vieillot) (type host) and Myiodynastes maculatus (Statius Muller) from Paraguay. The generic diagnosis of Anonchotaenia Conn, 1900 is amended, prompted by the presence of the armed cirrus and the elongated cirrus sac of A. prolixa. Two species were redescribed: Anonchotaenia brasiliensis Fuhrmann, 1908 from Tachyphonus coronatus (Vieillot) and Thraupis cyanoptera (Vieillot) (new host records) from Brazil, and Thraupis sayaca (Linnaeus) and Volatinia jacarina (Linnaeus) from Paraguay (new host and geographic records); and Anonchotaenia macrocephala Fuhrmann, 1908 from Tachycineta leucorrhoa (Vieillot) (new host record) from Brazil, Tachycineta meyeni (Cabanis) from Chile (new host and geographic record) and Stelgidopteryx ruficollis (Vieillot) from Paraguay (new host and geographic record). Scanning electron microscopy of A. brasiliensis and A. macrocephala revealed less microthrix variation than has been reported for other cyclophyllidean taxa. Sequence data were generated for nuclear ssr- and lsr-DNA and mitochondrial rrnL and cox1 for A. prolixa, A. brasiliensis, and A. macrocephala. Maximum likelihood and Bayesian inference analyses supported each species as distinct, but revealed cryptic diversity among A. brasiliensis specimens from different host families. New host records of A. brasiliensis and A. macrocephala prompted a formal assessment of host specificity. Anonchotaenia prolixa was found to be oioxenous (HS(S) = 0), A. vaslata and A. macrocephala were found to be metastenoxenous (HS(S) = 3.000 and 3.302, respectively), whereas A. brasiliensis was found to be euryxenous (HS(S) = 5.876). Anonchotaenia brasiliensis has been found parasitising several species of different passerine families that participate in mixed-species foraging flocks in the Atlantic Forest. A diversity of species of other families join these flocks and are among the substantial number of South American passerine species yet to be examined for cestodes.

An unusual form of reaction wood in Koromiko [Hebe salicifolia G. Forst. (Pennell)], a southern hemisphere angiosperm

Koromiko [Hebe salicifolia G. Forst. (Pennell)] is a woody angiosperm native to New Zealand and Chile. Hebe spp. belong to the otherwise herbaceous family Plantaginaceae in the order Lamiales. Reaction wood exerting expansional forces was found on the lower side of leaning H. salicifolia stems. Such reaction wood is atypical for angiosperms, which commonly form contracting reaction wood on the upper side of leaning stems. Reaction wood typical for angiosperms is formed by species in other families in the order Lamiales. This suggests that the form of reaction wood is specific to the family level. Functionally the reaction wood of H. salicifolia is similar to that found in gymnosperms, which both act by pushing. However, their chemical, anatomical and physical characteristics are different. Typical features of reaction wood present in gymnosperms such as high density, thick-walled rounded cells and the presence of (1 → 4)-β-galactan in the secondary cell wall layer are absent in H. salicifolia reaction wood. Reaction wood of H. salicifolia varies from normal wood in having a higher microfibril angle, which is likely to determine the direction of generated maturation stresses.

Phenylphenalenone phytoalexins, will they be a new type of fungicide?

Phenylphenalenones represent a kind of phytoalexins produced in leaves and rhyzomes of banana and plantains (Musaceae), as well as in species of other families. These compounds are synthesized in plants by induction with aminoglycosides, or in the first stages of attack by the pathogenic fungus Mycosphaerella fijensis, a causal agent of the disease known as Black Sigatoka, which reduces banana production. In this paper we report the biosynthesis, synthesis and antifungal activities of these kinds of compounds and discus the possibility to use phytoalexins inductors as plant protectants.

Natural Regeneration Potentials Of Some Indigenous Timber Species In An Exploited Plantation Site At Sapoba, Benin City, Nigeria

Seedlings and saplings of economic timber species encounted within an area of 4,000m2 at Sapoba, Edo State (which was previously a plantation site for Entandrophragma cylindricum, Khaya ivorensis and Lovoa trichilodes, established between 1930 and 1932, but were completely exploited between 1990 and 1992) were assessed in order to find out the economic timber species that had regenerated naturally within that land space. The results showed that only 18 choice timber species were encounted within the land area. Gossweilerodendran balsamifera, was significantly (p ≤ 0.05) the most abundant species (42.0%), while the Meliaceae and the Leguminaseae were significantly the most abundant families (p ≤ 0.05)encounted. These were Meliaceae: Entandrophragma angolense(16.5%), Lovoa trichiliodes(8.3%), khaya ivorensis (5.2%) , Entandrophragma cylindricum(3.4%), Guarea cedrata(2.0%), and G. thompsonii(1.0%) and Leguminosae. Albizia ferruginea (3.0%), A. lebbeck (0.6%), Berlinia grandiflora (1.0%), Daniela ogea (0.9%). Piptadeniastrum africana (4.2%) and G. balsamiferam (42.0%). Other species in other families encounterted were Antiaris africana (3.2%), Celtis zenkeri (0.8%), Diospyros mespiliformis (1.00%), Lophira alata (3.2%), Pycannthus angolensis (3.5%) and Terminalia ivorensis (0.5%). Collar diameter correlated significantly and positively with height (r =0.77), showing that generally, the taller the individual stem the larger the collar diameter. The significance of these findings in relation to conservation and management of the choice indigenous timber species to restore them to their pride of place both in Nigeria and abroad.

Production and quality of meadow associations in forest hunting grounds in Serbia

Determination of meadow associations was done on three locations: enclosed section of the hunting ground 'Posavsko loviste Karakusa' (Forest holding Sremska Mitrovica), enclosed breeding ground i.e. area 'Miloseva voda' (Sokolovica) and enclosed breeding ground 'Lomnicka reka' (Veliki Jastrebac). Phytocenological records were used to determine number and cover, and in samples the presence of plant species was determined and based on this analysis to which plant associations they belong. In determined associations the share in percentages of grass and leguminous species of high, medium and poor quality was determined, as well as species of other families, conditionally useful, bad, worthless, harmful and slightly poisonous. On the first location the following associations were established: Agrostio-Juncetum effusi Cinc. 1959., Trifolio-Agrostietum stoloniferae LJ. Mark. 1973., Agrostietum vulgaris Z. Pavl. 1955. sensu lato. On the second location, on both meadows, the association Festuco-Agrostietum Horv. (1952) 1982. em Trinajest. 1972. was established, whereas on the third location, association Agrostio-Festucetum vallesiacae Gajic 1961 was established. In investigated associations, the production of green mass and dry matter was established, as well as main parameters of the quality of grass mass (crude proteins, crude fibre, crude lipids, NFE and mineral matters). .

Host Specificity of <I>Argopistes tsekooni</I> (Coleoptera: Chrysomelidae), a Potential Biological Control Agent of Chinese Privet

Chinese privet, Ligustrum sinense Lour., is a perennial semi-evergreen shrub that is a serious invasive weed in the United States. Classical biological control offers the best hope for controlling it in an economic, effective, and persistent way. Host specificity of one of the most promising biological control agents of Chinese privet, a flea beetle, Argopistes tsekooni Chen (Coleoptera: Chrysomelidae), was evaluated in China by using laboratory no-choice and choice tests on 13 species of Oleaceae and eight species in other families that have important economic value. In adult no-choice survival and oviposition tests, the flea beetle fed and survived for 30 d on Syringa oblata Lindl., Jasminum nudiflorum Lindl., and three species in the genus Ligustrum. Females also oviposited on these species, but only larvae from eggs laid on S. oblata and Ligustrum spp. developed successfully. In addition, the beetles did not feed or oviposit on the species of economic importance. In choice tests, adults preferred L. sinense for feeding and oviposition. These results show that A. tsekooni is relatively host specific and warrants further testing as a biocontrol agent of Chinese privet in the United States.

Legume yellow mosaic viruses: genetically isolated begomoviruses

The yellow mosaic diseases of a number of legumes across Southern Asia are caused by four species of whitefly-transmitted geminiviruses (genus Begomovirus, family Geminiviridae): Mungbean yellow mosaic virus, Mungbean yellow mosaic India virus, Dolichos yellow mosaic virus and Horsegram yellow mosaic virus. They cause losses to a number of important pulse crops, a major source of dietary protein in the region. The viruses have host ranges limited to plants of the family Fabaceae and efforts to limit losses are hampered by limited availability of conventional resistance sources and/or the lack of durability of the resistance that has been identified. There is ample evidence for genetic interaction between these begomoviruses within the legumes, in the form of both classical recombination and component exchange, but little evidence for interaction with viruses that infect other plants. This is indicative of genetic isolation, the viruses in legumes evolving independently of the begomoviruses in plant species of other families. This has implications for the development of engineered resistance in legumes, which holds the promise of durability but has yet to be transferred to the field. The viruses causing yellow mosaic diseases of legumes across southern Asia, four of which have been identified so far, are bipartite begomoviruses (genus Begomovirus, family Geminiviridae): Mungbean yellow mosaic virus, Mungbean yellow mosaic India virus, Horsegram yellow mosaic virus and Dolichos yellow mosaic virus. Physical properties: The legume yellow mosaic viruses (LYMVs), like all members of the Geminiviridae, have geminate (twinned) particles, 18-20 nm in diameter, 30 nm long, apparently consisting of two incomplete T = 1 icosahedra joined together in a structure with 22 pentameric capsomers and 110 identical protein subunits. Symptoms caused by LYMVs are largely dependent on host species and susceptibility. Initially symptoms appear as small yellow specks along the veins and then spread over the leaf. In severe infections the entire leaf may become chlorotic. In blackgram the chlorotic areas sometimes turn necrotic. Infections of French bean usually do not produce a mosaic but instead induce a downward leaf curling. Control is based mainly on preventing the establishment of the whitefly vector, Bemisia tabaci, in the crop by application of insecticides. Changes in agricultural practices, such as moving the cropping period out of periods of high vector incidence (the wet period in late summer) to times of low vector incidence (dry season in early summer) have met with some, albeit short-term, benefits. The use of natural, host plant resistance is efficacious, although the available sources of resistance in most legume crops are limited. In mungbean the resistance is attributed to two recessive genes which are used effectively to control the disease. http://www.danforthcentre.org/iltab/geminiviridae/, http://www.iwglvv.org/

Species having C4 single-cell-type photosynthesis in the Chenopodiaceae family evolved a photosynthetic phosphoenolpyruvate carboxylase like that of Kranz-type C4 species.

Spatial and temporal regulation of phosphoenolpyruvate carboxylase (PEPC) is critical to the function of C(4) photosynthesis. The photosynthetic isoform of PEPC in the cytosol of mesophyll cells in Kranz-type C(4) photosynthesis has distinctive kinetic and regulatory properties. Some species in the Chenopodiaceae family perform C(4) photosynthesis without Kranz anatomy by spatial separation of initial fixation of atmospheric CO(2) via PEPC from C(4) acid decarboxylation and CO(2) donation to Rubisco within individual chlorenchyma cells. We studied molecular and functional features of PEPC in two single-cell functioning C(4) species (Bienertia sinuspersici, Suaeda aralocaspica) as compared to Kranz type (Haloxylon persicum, Salsola richteri, Suaeda eltonica) and C(3) (Suaeda linifolia) chenopods. It was found that PEPC from both types of C(4) chenopods displays higher specific activity than that of the C(3) species and shows kinetic and regulatory characteristics similar to those of C(4) species in other families in that they are subject to light/dark regulation by phosphorylation and display differential malate sensitivity. Also, the deduced amino acid sequence from leaf cDNA indicates that the single-cell functioning C(4) species possesses a Kranz-type C(4) isoform with a Ser in the amino terminal. A phylogeny of PEPC shows that isoforms in the two single-cell functioning C(4) species are in a clade with the C(3) and Kranz C(4) Suaeda spp. with high sequence homology. Overall, this study indicates that B. sinuspersici and S. aralocaspica have a C(4)-type PEPC similar to that in Kranz C(4) plants, which likely is required for effective function of C(4) photosynthesis.

Nitric oxide synthase type I (nNOS), vascular endothelial growth factor (VEGF) and myoglobin-like expression in skeletal muscle of Antarctic icefishes (Notothenioidei: Channichthyidae)

The Antarctic icefishes Channichthyidae lack haemoglobin and are thought to lack myoglobin (Mb) in their skeletal muscle as well. Due to the absence of both respiratory pigments, icefishes may present a variety of physiological adaptations in their skeletal muscles. In mammals, molecular responses to limiting oxygen availability in the skeletal muscle include, among others, the over expression of nitric oxide synthases (NOS), such as type I (neuronal nNOS) and type III (endothelial eNOS), as well as the vascular endothelial growth factor (VEGF). In this paper, we evaluated by western blot analysis whether the skeletal muscle of haemoglobin-less icefishes expresses in a constitutive manner higher levels of the type I and type III NOS isoforms and VEGF. Our results demonstrate that haemoglobin-less icefish of the family Channichthyidae do indeed present higher expression of the type I NOS isoform compared with red-blooded Antarctic fish species of other families of the same suborder Notothenioidei. In contrast, VEGF was not over-expressed. Moreover, we show that some icefish species, thought previously to lack Mb in oxidative muscles, actually present Mb-like immunoreactivity in their skeletal muscle.

Detecção de Azospirillum amazonense em raízes e rizosfera de orchidaceae e de outras famílias vegetais

Azospirillum amazonense é uma bactéria fixadora de N2 atmosférico de ampla ocorrência, principalmente em associações radiculares com gramíneas e palmeiras. Para verificar sua presença em outras espécies vegetais, ainda não estudadas, e a eficiência de meios para sua detecção, foram testados os meios Fam e LGI para contagens em solo rizosférico ou em culturas de enriquecimento com solo rizosférico, ecto e endorrizosfera. A. amazonense foi detectada no solo rizosférico, ecto e endorrizosfera de várias espécies de monocotiledôneas, incluindo Orchidaceae e dicotiledôneas, sendo o meio Fam mais eficiente para sua detecção

Life History and Case-Building Behavior of <I>Phylloicus ornatus</I> (Trichoptera: Calamoceratidae) in Two Spring-Fed Streams in Texas

The life history and case-making behavior of Phylloicus ornatus (Banks) from two spring-fed first-order streams in the Edwards Plateau bioregion of Texas were studied from January 1998 to November 1999. Field sampling of larvae, pupae, and adults, and laboratory rearings indicated a multivoltine cycle. Eggs averaged 0.32 mm diameter and have distinctive, ridged chorionic sculpturing. First instars differ from late instars in having fewer labral setae and a unique spur-like claw on each lateral hump. Larval development was asynchronous, with second through fifth instars and pupae present most months. First instars were present April through July, October, and November. Case-making of first instars, and case reconstruction of later instars extracted from their cases, was documented by videotape. The chronological aspects of case-building behavior of first and later instars are documented. Case-building was compared with that of two other species in other families. The near-identical sequences suggest that case-making behavior in all three species represents a recapitulation of evolutionary events leading to larval case-making in Spicipalpia and Integripalpia.

The oldest known catfish (Teleostei: Siluriformes) from Asia (India, Late Cretaceous)

Siluriformes constitute an important monophyletic group of mainly freshwater fishes (Fink and Fink, 1996). Presently, there are about 35 families with over 2,000 species in all continents except Antarctica (Lundberg, 1993; Nelson, 1994); however, at least in the Eocene, they also inhabited Antarctica as well (Grande and Eastman, 1986). A small number of catfishes (most of the ariids and many of the plotosids) are marine and some species of other families can enter brackish waters (Nelson, 1994). Pre-Cenozoic catfishes are extremely rare outside of South America.