Using historical data of climate, land-use, hydrology and water quality from four catchments located in the south of England, this study identifies the impact of climate and land-use change on selected water quantity and water quality indicators. The study utilises a paired catchment approach, with two catchments that have experienced a high degree of urbanisation over the past five decades and two nearby, hydrologically similar, but undeveloped catchments. Multivariate regression models were used to assess the influence of rainfall and urbanisation on runoff (annual and seasonal), dissolved oxygen levels and temperature. Results indicate: (i) no trend in annual or seasonal rainfall totals, (ii) upward trend in runoff totals in the two urban catchments but not in the rural catchments, (iii) upward trend in dissolved oxygen and temperature in the urban catchments, but not in the rural catchments, and (iv) changes in temperature and dissolved oxygen in the urban catchments are not driven by climatic variables.